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2022 (English)In: New Biotechnology, ISSN 1871-6784, E-ISSN 1876-4347, Vol. 72, p. 139-148Article in journal (Refereed) Published
Abstract [en]
A homogeneous PCR-based assay for sensitive and specific detection of antibodies in serum or dried blood spots (DBS) is presented and the method is used to monitor individuals infected with or vaccinated against SARS-CoV-2. Detection probes were prepared by conjugating the recombinant spike protein subunit 1 (S1), containing the receptor binding domain (RBD) of SARS-CoV-2, to each of a pair of specific oligonucleotides. The same was done for the nucleocapsid protein (NP). Upon incubation with serum or DBS samples, the bi- or multivalency of the antibodies (IgG, IgA or IgM) brings pairs of viral proteins with their conjugated oligonucleotides in proximity, allowing the antibodies to be detected by a modified proximity extension assay (PEA). Anti-S1 and anti-NP antibodies could be detected simultaneously from one incubation reaction. This Antibody PEA (AbPEA) test uses only 1 µl of neat or up to 100,000-fold diluted serum or one ø1.2 mm disc cut from a DBS. All 100 investigated sera and 21 DBS collected prior to the COVID-19 outbreak were negative, demonstrating a 100% specificity. The area under the curve, as evaluated by Receiver Operating Characteristic (ROC) analysis reached 0.998 (95%CI: 0.993–1) for samples taken from 11 days after symptoms onset. The kinetics of antibody responses were monitored after a first and second vaccination using serially collected DBS from 14 individuals. AbPEA offers highly specific and sensitive solution-phase antibody detection without requirement for secondary antibodies, no elution step when using DBS sample in a simple procedure that lends itself to multiplex survey of antibody responses.
Place, publisher, year, edition, pages
Elsevier, 2022
Keywords
Homogenous serological assay, PCR-based antibody detection, SARS-CoV-2 antibody, Proximity extension assay, Finger -prick dried blood spot, Multiplex immunoassay
National Category
Biochemistry and Molecular Biology Infectious Medicine Immunology in the medical area
Identifiers
urn:nbn:se:uu:diva-492677 (URN)10.1016/j.nbt.2022.11.004 (DOI)000896515300004 ()36423830 (PubMedID)
Funder
Science for Life Laboratory, SciLifeLabKnut and Alice Wallenberg Foundation, 2020.0182Swedish Foundation for Strategic Research, SB16-0046Swedish Research Council, 2020-02258
2023-01-102023-01-102024-02-01Bibliographically approved