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  • Höglund, Arja
    et al.
    Karolinska Inst, Dept Clin Neurosci, Stockholm, Sweden;Karolinska Univ, Hosp Huddinge, Dept Neurol, Stockholm, Sweden.
    Hagell, Peter
    Kristianstad Univ, PRO CARE Grp, Fac Hlth Sci, Kristianstad, Sweden.
    Broman, Jan-Erik
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Lung- allergi- och sömnforskning. Uppsala Univ, Dept Neurosci, Psychiat, Uppsala, Sweden.
    Pålhagen, Sven
    Karolinska Inst, Dept Clin Neurosci, Stockholm, Sweden;Karolinska Univ, Hosp Huddinge, Dept Neurol, Stockholm, Sweden.
    Sorjonen, Kimmo
    Karolinska Inst, Dept Clin Neurosci, Stockholm, Sweden.
    Fredrikson, Sten
    Karolinska Inst, Dept Clin Neurosci, Stockholm, Sweden;Karolinska Univ, Hosp Huddinge, Dept Neurol, Stockholm, Sweden.
    A 10-Year Follow-Up of Excessive Daytime Sleepiness in Parkinson's Disease2019Inngår i: Parkinson's Disease, ISSN 2090-8083, E-ISSN 2042-0080, Vol. 2019, artikkel-id 5708515Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Introduction. The aim of this prospective study was to investigate excessive daytime sleepiness (EDS) over time and in relation to other PD symptoms among people with Parkinson's disease (PD). Methods. Thirty participants younger than 65 years with PD were randomly selected. At inclusion, mean (SD) disease duration was 6.2 (4.8) years and median (min-max) severity of PD was classified as stage II (stages I-III) according to Hoehn and Yahr. Participants were followed annually for 10 years with clinical assessments of their PD status, medications, comorbidities, and a standardized interview about their sleep habits and occurrence of daytime sleepiness. EDS was assessed by the self-reported Epworth Sleepiness Scale (ESS). Seventeen participants completed the 10-year longitudinal follow-up. Results. Fifteen of 30 persons were classified to suffer from EDS (ESS > 10) at baseline. At the group level, EDS remained stable over 10 years and did not deteriorate in parallel with worsening of motor symptoms. Furthermore, EDS was associated with sleep quality, fatigue, anxiety, depression, and axial/postural/gait impairments. Conclusions. EDS did not worsen over 10 years, although other PD aspects did. EDS in PD seems to be a complex nonmotor symptom that is unrelated to deterioration of motor symptoms in PD.

  • Berg, Elena C.
    et al.
    Amer Univ Paris, Dept Comp Sci Math & Environm Sci, Paris, France.
    Lind, Martin I.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för ekologi och genetik, Zooekologi.
    Monahan, Shannon
    Amer Univ Paris, Dept Comp Sci Math & Environm Sci, Paris, France.
    Bricout, Sophie
    Amer Univ Paris, Dept Comp Sci Math & Environm Sci, Paris, France.
    Maklakov, Alexei A.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för ekologi och genetik, Zooekologi.
    Kin but less than kind: within-group male relatedness does not increase female fitness in seed beetles2019Inngår i: Proceedings of the Royal Society of London. Biological Sciences, ISSN 0962-8452, E-ISSN 1471-2954, Vol. 286, nr 1910, s. 1471-2954, artikkel-id 20191664Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Theory maintains within-group male relatedness can mediate sexual conflict by reducing male-male. competition and collateral harm to females. We tested whether male relatedness can lessen female harm in the seed beetle Callosobruchus maculatus. Male relatedness did not influence female lifetime reproductive success or individual fitness across two different ecologically relevant scenarios of mating competition. However, male relatedness marginally improved female survival. Because male relatedness improved female-survival in late life when C. maculatus females are no longer producing offspring, our results do not provide support for the role of within-group male relatedness in mediating sexual conflict. The fact that male relatedness improves the post-reproductive part of the female life cycle strongly suggests that the effect is non-adaptive. We discuss adaptive and non-adaptive mechanisms that could result in reduced female harm in this and previous studies, and suggest that cognitive error is a likely explanation.

  • Disputas: 2019-11-11 09:00 C2:305, Biomedical Centrum (BMC), Uppsala
    Diamanti, Klev
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för cell- och molekylärbiologi, Beräkningsbiologi och bioinformatik.
    Integrating multi-omics for type 2 diabetes: Data science and big data towards personalized medicine2019Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    Type 2 diabetes (T2D) is a complex metabolic disease characterized by multi-tissue insulin resistance and failure of the pancreatic β-cells to secrete sufficient amounts of insulin. Cells recruit transcription factors (TF) to specific genomic loci to regulate gene expression that consequently affects the protein and metabolite abundancies. Here we investigated the interplay of transcriptional and translational regulation, and its impact on metabolome and phenome for several insulin-resistant tissues from T2D donors. We implemented computational tools and multi-omics integrative approaches that can facilitate the selection of candidate combinatorial markers for T2D.

    We developed a data-driven approach to identify putative regulatory regions and TF-interaction complexes. The cell-specific sets of regulatory regions were enriched for disease-related single nucleotide polymorphisms (SNPs), highlighting the importance of such loci towards the genomic stability and the regulation of gene expression. We employed a similar principle in a second study where we integrated single nucleus ribonucleic acid sequencing (snRNA-seq) with bulk targeted chromosome-conformation-capture (HiCap) and mass spectrometry (MS) proteomics from liver. We identified a putatively polymorphic site that may contribute to variation in the pharmacogenetics of fluoropyrimidines toxicity for the DPYD gene. Additionally, we found a complex regulatory network between a group of 16 enhancers and the SLC2A2 gene that has been linked to increased risk for hepatocellular carcinoma (HCC). Moreover, three enhancers harbored motif-breaking mutations located in regulatory regions of a cohort of 314 HCC cases, and were candidate contributors to malignancy.

    In a cohort of 43 multi-organ donors we explored the alternating pattern of metabolites among visceral adipose tissue (VAT), pancreatic islets, skeletal muscle, liver and blood serum samples. A large fraction of lysophosphatidylcholines (LPC) decreased in muscle and serum of T2D donors, while a large number of carnitines increased in liver and blood of T2D donors, confirming that changes in metabolites occur in primary tissues, while their alterations in serum consist a secondary event. Next, we associated metabolite abundancies from 42 subjects to glucose uptake, fat content and volume of various organs measured by positron emission tomography/magnetic resonance imaging (PET/MRI). The fat content of the liver was positively associated with the amino acid tyrosine, and negatively associated with LPC(P-16:0). The insulin sensitivity of VAT and subcutaneous adipose tissue was positively associated with several LPCs, while the opposite applied to branch-chained amino acids. Finally, we presented the network visualization of a rule-based machine learning model that predicted non-diabetes and T2D in an “unseen” dataset with 78% accuracy.

    Delarbeid
    1. Maps of context-dependent putative regulatory regions and genomic signal interactions
    Åpne denne publikasjonen i ny fane eller vindu >>Maps of context-dependent putative regulatory regions and genomic signal interactions
    Vise andre…
    2016 (engelsk)Inngår i: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 44, nr 19, s. 9110-9120Artikkel i tidsskrift (Fagfellevurdert) Published
    Abstract [en]

    Gene transcription is regulated mainly by transcription factors (TFs). ENCODE and Roadmap Epigenomics provide global binding profiles of TFs, which can be used to identify regulatory regions. To this end we implemented a method to systematically construct cell-type and species-specific maps of regulatory regions and TF-TF interactions. We illustrated the approach by developing maps for five human cell-lines and two other species. We detected similar to 144k putative regulatory regions among the human cell-lines, with the majority of them being similar to 300 bp. We found similar to 20k putative regulatory elements in the ENCODE heterochromatic domains suggesting a large regulatory potential in the regions presumed transcriptionally silent. Among the most significant TF interactions identified in the heterochromatic regions were CTCF and the cohesin complex, which is in agreement with previous reports. Finally, we investigated the enrichment of the obtained putative regulatory regions in the 3D chromatin domains. More than 90% of the regions were discovered in the 3D contacting domains. We found a significant enrichment of GWAS SNPs in the putative regulatory regions. These significant enrichments provide evidence that the regulatory regions play a crucial role in the genomic structural stability. Additionally, we generated maps of putative regulatory regions for prostate and colorectal cancer human cell-lines.

    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-310761 (URN)10.1093/nar/gkw800 (DOI)000388016900012 ()27625394 (PubMedID)
    Forskningsfinansiär
    AstraZenecaSwedish Research CouncilSwedish Diabetes AssociationeSSENCE - An eScience Collaboration
    Merknad

    De två första författarna delar förstaförfattarskapet.

    Tilgjengelig fra: 2016-12-19 Laget: 2016-12-19 Sist oppdatert: 2019-09-22bibliografisk kontrollert
    2. Single Nuclei Transcriptome Analysis of Human Liver with Integration of Proteomics and Capture Hi-C Bulk Tissue Data
    Åpne denne publikasjonen i ny fane eller vindu >>Single Nuclei Transcriptome Analysis of Human Liver with Integration of Proteomics and Capture Hi-C Bulk Tissue Data
    Vise andre…
    (engelsk)Inngår i: Artikkel i tidsskrift (Fagfellevurdert) Submitted
    Abstract [en]

    The liver is the largest solid organ and a primary metabolic hub. In recent years, intact cell nuclei were used to perform single-nuclei RNA-seq (snRNA-seq) for tissues difficult to dissociate and for flash-frozen archived tissue samples to discover unknown and rare cell sub-populations. In this study, we performed snRNA-seq of a liver sample to identify sub-populations of cells based on nuclear transcriptomics. In 4,282 single nuclei we detected on average 1,377 active genes and we identified seven major cell types. We integrated data from 94,286 distal interactions (p<0.05) for 7,682 promoters from a targeted chromosome conformation capture technique (HiCap) and mass spectrometry (MS) proteomics for the same liver sample. We observed a reasonable correlation between proteomics and in silico bulk snRNA-seq (r=0.47) using tissue-independent gene-specific protein abundancy estimation factors. We specifically looked at genes of medical importance. The DPYD gene is involved in the pharmacogenetics of fluoropyrimidines toxicity and some of its variants are analyzed for clinical purposes. We identified a new putative polymorphic regulatory element, which may contribute to variation in toxicity. Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer and we investigated all known risk genes. We found a complex regulatory network for the SLC2A2 gene with 16 candidate enhancers. Three of them harbor somatic motif breaking and other mutations in HCC in the Pan Cancer Analysis of Whole Genomes dataset and are candidates to contribute to malignancy. Our results highlight the potential of a multi-omics approach in the study of human diseases.

    Emneord
    multi-omics; snRNA-seq; MS proteomics; TAD; HiCap; liver; DPYD; SLC2A2
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-393431 (URN)
    Tilgjengelig fra: 2019-09-21 Laget: 2019-09-21 Sist oppdatert: 2019-09-22
    3. Intra- and inter-individual metabolic profiling highlights carnitine and lysophosphatidylcholine pathways as key molecular defects in type 2 diabetes
    Åpne denne publikasjonen i ny fane eller vindu >>Intra- and inter-individual metabolic profiling highlights carnitine and lysophosphatidylcholine pathways as key molecular defects in type 2 diabetes
    Vise andre…
    2019 (engelsk)Inngår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, artikkel-id 9653Artikkel i tidsskrift (Fagfellevurdert) Published
    Abstract [en]

    Type 2 diabetes (T2D) mellitus is a complex metabolic disease commonly caused by insulin resistance in several tissues. We performed a matched two-dimensional metabolic screening in tissue samples from 43 multi-organ donors. The intra-individual analysis was assessed across five key metabolic tissues (serum, visceral adipose tissue, liver, pancreatic islets and skeletal muscle), and the inter-individual across three different groups reflecting T2D progression. We identified 92 metabolites differing significantly between non-diabetes and T2D subjects. In diabetes cases, carnitines were significantly higher in liver, while lysophosphatidylcholines were significantly lower in muscle and serum. We tracked the primary tissue of origin for multiple metabolites whose alterations were reflected in serum. An investigation of three major stages spanning from controls, to pre-diabetes and to overt T2D indicated that a subset of lysophosphatidylcholines was significantly lower in the muscle of pre-diabetes subjects. Moreover, glycodeoxycholic acid was significantly higher in liver of pre-diabetes subjects while additional increase in T2D was insignificant. We confirmed many previously reported findings and substantially expanded on them with altered markers for early and overt T2D. Overall, the analysis of this unique dataset can increase the understanding of the metabolic interplay between organs in the development of T2D.

    sted, utgiver, år, opplag, sider
    NATURE PUBLISHING GROUP, 2019
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-391017 (URN)10.1038/s41598-019-45906-5 (DOI)000474222900010 ()31273253 (PubMedID)
    Forskningsfinansiär
    AstraZenecaSwedish Research Council FormaseSSENCE - An eScience CollaborationSwedish Diabetes AssociationErnfors Foundation
    Tilgjengelig fra: 2019-08-21 Laget: 2019-08-21 Sist oppdatert: 2019-09-22bibliografisk kontrollert
    4. Integration of whole-body PET/MRI with non-targeted metabolomics provides new insights into insulin sensitivity of various tissues
    Åpne denne publikasjonen i ny fane eller vindu >>Integration of whole-body PET/MRI with non-targeted metabolomics provides new insights into insulin sensitivity of various tissues
    Vise andre…
    (engelsk)Manuskript (preprint) (Annet vitenskapelig)
    Abstract [en]

    Background: Alteration of various metabolites has been linked to type 2 diabetes (T2D) and insulin resistance. However, identifying significant associations between metabolites and tissue-specific alterations is challenging and requires a multi-omics approach. In this study, we aimed at discovering associations of metabolites from subcutaneous adipose tissue (SAT) and plasma with the volume, the fat fraction (FF) and the insulin sensitivity (Ki) of specific tissues using [18F]FDG PET/MRI.

    Materials and Methods: In a cohort of 42 subjects with different levels of glucose tolerance (normal, prediabetes and T2D) matched for age and body-mass-index (BMI) we calculated associations between parameters of whole-body FDG PET/MRI during clamp and non-targeted metabolomics profiling for SAT and blood plasma. We also used a rule-based classifier to identify a large collection of prevalent patterns of co-dependent metabolites that characterize non-diabetes (ND) and T2D.

    Results: The plasma metabolomics profiling revealed that hepatic fat content was positively associated with tyrosine, and negatively associated with lysoPC(P-16:0). Ki in visceral adipose tissue (VAT) and SAT, was positively associated with several species of lysophospholipids while the opposite applied to branched-chain amino acids (BCAA) and their intermediates. The adipose tissue metabolomics revealed a positive association between non-esterified fatty acids and, VAT and liver Ki. On the contrary, bile acids and carnitines in adipose tissue were inversely associated with VAT Ki. Finally, we presented a transparent machine-learning model that predicted ND or T2D in “unseen” data with an accuracy of 78%.

    Conclusions: Novel associations of several metabolites from SAT and plasma with the FF, volume and insulin senstivity of various tissues throughout the body were discovered using PET/MRI and a new integrative multi-omics approach. A promising computational model that predicted ND and T2D with high certainty, suggested novel non-linear interdependencies of metabolites.

    Emneord
    type 2 diabetes; metabolomics; imiomics; PET/MRI; insulin resistance;
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-393429 (URN)
    Tilgjengelig fra: 2019-09-21 Laget: 2019-09-21 Sist oppdatert: 2019-09-22
  • Tyson, Yosef
    et al.
    Uppsala Univ Hosp, Dept Surg Sci, Sect Orthoped Surg, Uppsala, Sweden;Swedish Hip Arthroplasty Register, Gothenburg, Sweden.
    Rolfson, Ola
    Swedish Hip Arthroplasty Register, Gothenburg, Sweden;Sahlgrens Univ Hosp, Sahlgrenska Acad, Inst Clin Sci, Dept Orthoped, Molndal, Sweden.
    Karrholm, Johan
    Swedish Hip Arthroplasty Register, Gothenburg, Sweden;Sahlgrens Univ Hosp, Sahlgrenska Acad, Inst Clin Sci, Dept Orthoped, Molndal, Sweden.
    Hailer, Nils
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kirurgiska vetenskaper, Ortopedi.
    Mohaddes, Maziar
    Swedish Hip Arthroplasty Register, Gothenburg, Sweden;Sahlgrens Univ Hosp, Sahlgrenska Acad, Inst Clin Sci, Dept Orthoped, Molndal, Sweden.
    Uncemented or cemented revision stems?: Analysis of 2,296 first-time hip revision arthroplasties performed due to aseptic loosening, reported to the Swedish Hip Arthroplasty Register2019Inngår i: Acta Orthopaedica, ISSN 1745-3674, E-ISSN 1745-3682, Vol. 90, nr 5, s. 421-426Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background and purpose - Uncemented stems are increasingly used in revision hip arthroplasty, but only a few studies have analyzed the outcomes of uncemented and cemented revision stems in large cohorts of patients. We compared the results of uncemented and cemented revision stems. Patients and methods - 1,668 uncemented and 1,328 cemented revision stems used in first-time revisions due to aseptic loosening between 1999 and 2016 were identified in the Swedish Hip Arthroplasty Register. Kaplan-Meier analysis was used to investigate unadjusted implant survival with re-revision for any reason as the primary outcome. Hazard ratios (HR) for the risk of re-revision were calculated using a Cox regression model adjusted for sex, age, head size, concomitant cup revision, surgical approach at primary and at index revision surgery, and indication for primary total hip arthroplasty. Results - Unadjusted 10-year survival was 85% (95% CI 83-87) for uncemented and 88% (CI 86-90) for cemented revision stems. The adjusted HR for re-revision of uncemented revision stems during the first year after surgery was 1.3 (CI 1.0-1.6), from the second year the HR was 1.1 (CI 0.8-1.4). Uncemented stems were most often re-revised early due to infection and dislocation, whereas cemented stems were mostly re-revised later due to aseptic loosening. Interpretation - Both uncemented and cemented revision stems had satisfactory long-term survival but they differed in their modes of failure. Our conclusions are limited by the fact that femoral bone defect size could not be investigated within the setting of the current study.

  • Sayed, Fatima
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets fysik.
    Muscas, Giuseppe
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi.
    Jovanovic, S.
    Jozef Stefan Inst, Adv Mat Dept, Ljubljana 1000, Slovenia;Univ Belgrade, Vinca Inst Nucl Sci, Lab Phys, Belgrade 11000, Serbia.
    Barucca, G.
    Univ Politecn Marche, Dept SIMAU, Via Brecce Bianche, I-60131 Ancona, Italy.
    Locardi, F.
    Univ Genoa, Dipartimento Chim & Chim Ind, Via Dodecaneso 31, I-16146 Genoa, Italy.
    Varvaro, G.
    CNR, Ist Struttura Mat, Area Ric Roma1, I-00015 Monterotondo, RM, Italy.
    Peddis, D.
    Univ Genoa, Dipartimento Chim & Chim Ind, Via Dodecaneso 31, I-16146 Genoa, Italy;CNR, Ist Struttura Mat, Area Ric Roma1, I-00015 Monterotondo, RM, Italy.
    Mathieu, Roland
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets fysik.
    Sarkar, Tapati
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets fysik.
    Controlling magnetic coupling in bi-magnetic nanocomposites2019Inngår i: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 11, nr 30, s. 14256-14265Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Magnetic nanocomposites constitute a vital class of technologically relevant materials, in particular for next-generation applications ranging from biomedicine, catalysis, and energy devices. Key to designing such materials is determining and controlling the extent of magnetic coupling in them. In this work, we show how the magnetic coupling in bi-magnetic nanocomposites can be controlled by the growth technique. Using four different synthesis strategies to prepare prototypical LaFeO3-CoFe2O4 and LaFeO3-Co0.5Zn0.5Fe2O4 nanocomposite systems, and by performing comprehensive magnetic measurements, we demonstrate that the final material exhibits striking differences in their magnetic coupling that is distinct to the growth method. Through structural and morphological studies, we confirm the link between the magnetic coupling and growth methods due to distinct levels of particle agglomeration at the very microscopic scale. Our studies reveal an inverse relationship between the strength of magnetic coupling and the degree of particle agglomeration in the nanocomposites. Our work presents a basic concept of controlling the particle agglomeration to tune magnetic coupling, relevant for designing advanced bi-magnetic nanocomposites for novel applications.

  • Sharma, Vaibhav
    et al.
    All India Inst Med Sci, Dept Biophys, New Delhi 110029, India.
    Rani, Komal
    All India Inst Med Sci, Dept Biophys, New Delhi 110029, India.
    Roychoudhury, Ajoy
    All India Inst Med Sci, CDER, Dept Oral & Maxillofacial Surg, New Delhi 110029, India.
    Chawla, Amita
    All India Inst Med Sci, Dept Biophys, New Delhi 110029, India.
    Nikolajeff, Fredrik
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Tillämpad materialvetenskap.
    Kumar, Saroj
    All India Inst Med Sci, Dept Biophys, New Delhi 110029, India.
    Novel Insights into Regulation of Human Teeth Biomineralization: Deciphering the Role of Post-Translational Modifications in a Tooth Protein Extract2019Inngår i: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 20, nr 16, artikkel-id 4035Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The importance of whole protein extracts from different types of human teeth in modulating the process of teeth biomineralization is reported. There are two crucial features in protein molecules that result in efficient teeth biomineralization. Firstly, the unique secondary structure characteristics within these proteins i.e. the exclusive presence of a large amount of intrinsic disorder and secondly, the presence of post-translational modifications (PTM) like phosphorylation and glycosylation within these protein molecules. The present study accesses the structural implications of PTMs in the tooth proteins through scanning electron microscopy and transmission electron microscopy. The deglycosylated/dephosphorylated protein extracts failed to form higher-order mineralization assemblies. Furthermore, through nanoparticle tracking analysis (NTA) we have shown that dephosphorylation and deglycosylation significantly impact the biomineralization abilities of the protein extract and resulted in smaller sized clusters. Hence, we propose these post-translational modifications are indispensable for the process of teeth biomineralization. In addition to basic science, this study would be worth consideration while designing of biomimetics architecture for an efficient peptide-based teeth remineralization strategy.

  • Kwak, Hee-Jin
    et al.
    Chungbuk Natl Univ, Coll Nat Sci, Sch Biol Sci, Cheongju 28644, Chungbuk, South Korea.
    Park, Jeong-Su
    Chungbuk Natl Univ, Coll Nat Sci, Sch Biol Sci, Cheongju 28644, Chungbuk, South Korea;GIST, Sch Life Sci, Gwangju 61005, South Korea.
    Jimenez, Brenda Irene Medina
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Geovetenskapliga sektionen, Institutionen för geovetenskaper, Paleobiologi. Chungbuk Natl Univ, Coll Nat Sci, Sch Biol Sci, Cheongju 28644, Chungbuk, South Korea.
    Park, Soon Cheol
    Chung Ang Univ, Dept Life Sci, Seoul 156756, South Korea.
    Cho, Sung-Jin
    Chungbuk Natl Univ, Coll Nat Sci, Sch Biol Sci, Cheongju 28644, Chungbuk, South Korea.
    Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches2019Inngår i: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 20, nr 16, artikkel-id 3994Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Antistasin, which was originally discovered in the salivary glands of the Mexican leech Haementeria officinalis, was newly isolated from Helobdella austinensis. To confirm the temporal expression of antistasin during embryogenesis, we carried out semi-quantitative RT-PCR. Hau-antistasin1 was uniquely expressed at stage 4 of the cleavage and was strongly expressed in the late stages of organogenesis, as were other antistasin members. In order to confirm the spatial expression of antistasin, we performed fluorescence in situ hybridization in the late stages of organogenesis. The expression of each antistasin in the proboscis showed a similar pattern and varied in expression in the body. In addition, the spatial expression of antistasin orthologs in different leeches showed the possibility of different function across leech species. Hau-antistasin1 was expressed in the same region as hedgehog, which is a known mediator of signal transduction pathway. Hau-antistasin1 is probably a downstream target of Hedgehog signaling, involved in segment polarity signal pathway.

  • Johansson, Cecilia
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Klinisk mikrobiologi.
    Nilsson, Anna
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Klinisk mikrobiologi. Swedish Univ Agr Sci, Dept Ecol, Uppsala, Sweden.
    Kaden, Rene
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Klinisk mikrobiologi.
    Rautelin, Hilpi
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Klinisk mikrobiologi.
    Differences in virulence gene expression between human blood and stool Campylobacter coli clade 1 ST828CC isolates2019Inngår i: Gut Pathogens, ISSN 1757-4749, Vol. 11, nr 1, artikkel-id 42Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background: Campylobacter colonise the gastrointestinal tract of warm-blooded animals and are major enteropathogens in humans. C. coli is less common than C. jejuni and accounts for about 10% of the total number of Campylobacter infections although the two species seem to share many virulence determinants. Campylobacter bacteraemia is rare, estimated to occur in less than 1% of the infections, and the exact mechanisms regulating the progression of the infection from the gastrointestinal tract to the blood stream are unclear. Here, we looked at the contribution of C. coli to Campylobacter infections and further compared various virulence traits in C. coli clade 1 blood and stool isolates. Results: We assessed the numbers of C. jejuni and C. coli among typed isolates in the PubMLST database and found that C. coli accounted for 25.9% of blood isolates, but only 8.9% of the stool isolates. Phylogenetic analysis of 128 C. coli clade 1 whole genome sequences deposited to NCBI revealed no specific clustering of the human blood, stool or animal isolates. Of the six C. coli isolates chosen for phenotypic analyses, stool isolates adhered significantly better to human HT-29 colon cancer cells than the blood isolates, while there was no difference in induced IL-8 levels between the isolates. Furthermore, the stool isolates had two-to fourfold higher RNA expression levels of the flpA, ciaB, iamA and cdt virulence genes than the blood isolates. Finally, we looked at the gene structure of the cdtA, B and C toxin genes and found numerous nucleotide additions and deletions disrupting the open reading frames. In contrast to 58% isolates of animal origin, only 38% and 32% of human blood and stool isolates, respectively, had all three cdt genes intact, a prerequisite to produce functional toxins. Conclusions: This study reveals interesting differences between C. coli clade 1 isolates of human and animal origin on one hand, and also between human blood and stool isolates, on the other. The results suggest that C. coli might downregulate and/or inactivate various virulence determinants as the isolates pass from the animal host to the human gastrointestinal tract and enter the human blood stream.

  • Skogsdal, Yvonne
    et al.
    Orebro Univ, Fac Med & Hlth, Maternal Hlth Care Unit, Orebro, Sweden.
    Fadl, Helena
    Orebro Univ, Fac Med & Hlth, Dept Obstet & Gynecol, Orebro, Sweden.
    Cao, Yang
    Orebro Univ, Sch Med Sci, Clin Epidemiol & Biostat, Biostat, Orebro, Sweden.
    Karlsson, Jan
    Orebro Univ, Fac Med & Hlth, Univ Hlth Care Res Ctr, Orebro, Sweden.
    Tydén, Tanja
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kvinnors och barns hälsa, Forskargrupper (Inst. för kvinnor och barns hälsa), Obstetrisk och reproduktiv hälsoforskning.
    An intervention in contraceptive counseling increased the knowledge about fertility and awareness of preconception health-a randomized controlled trial2019Inngår i: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 124, nr 3, s. 203-212Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background: Reproductive life plan counseling (RLPC) is a tool to encourage women and men to reflect upon their reproduction, to avoid unintended pregnancies and negative health behavior that can threaten reproduction. The aim was to evaluate the effect of RLPC among women attending contraceptive counseling. Outcomes were knowledge about fertility and awareness of preconception health, use of contraception, and women's experience of RLPC. Material and methods: Swedish-speaking women, aged 20-40 years, were randomized to intervention group (IG) or control group (CG). Participants (n = 1,946) answered a questionnaire before and two months after (n = 1,198, 62%) the consultation. All women received standard contraceptive counseling, and the IG also received the RLPC, i.e. questions on reproductive intentions, information about fertility, and preconception health. Results: Women in the IG increased their knowledge about fertility: age and fertility, chances of getting pregnant, fecundity of an ovum, and chances of having a child with help of IVF. They also increased their awareness of factors affecting preconception health, such as to stop using tobacco, to refrain from alcohol, to be of normal weight, and to start with folic acid before a pregnancy. The most commonly used contraceptive method was combined oral contraceptives, followed by long-acting reversible contraception. Three out of four women (76%) in the IG stated that the RLPC should be part of the routine in contraceptive counseling. Conclusions: Knowledge about fertility and awareness of preconception health increased after the intervention. The RLPC can be recommended as a tool in contraceptive counseling.

  • Qian, Zhao
    et al.
    Shandong Univ, Minist Educ, Key Lab Liquid Solid Struct Evolut & Proc Mat, Shenzhen 518057, Peoples R China;Shandong Univ, Shenzhen Inst, Shenzhen 518057, Peoples R China.
    Zhang, Hongni
    Shandong Management Univ, Jinan 250100, Shandong, Peoples R China.
    Jiang, Guanzhong
    Shandong Univ, Minist Educ, Key Lab Liquid Solid Struct Evolut & Proc Mat, Shenzhen 518057, Peoples R China;Shandong Univ, Shenzhen Inst, Shenzhen 518057, Peoples R China.
    Bai, Yanwen
    Shandong Univ, Minist Educ, Key Lab Liquid Solid Struct Evolut & Proc Mat, Shenzhen 518057, Peoples R China;Shandong Univ, Shenzhen Inst, Shenzhen 518057, Peoples R China.
    Ren, Yingying
    Shandong Univ, Minist Educ, Key Lab Liquid Solid Struct Evolut & Proc Mat, Shenzhen 518057, Peoples R China;Shandong Univ, Shenzhen Inst, Shenzhen 518057, Peoples R China.
    Du, Wenzheng
    Shandong Univ, Minist Educ, Key Lab Liquid Solid Struct Evolut & Proc Mat, Shenzhen 518057, Peoples R China;Shandong Univ, Shenzhen Inst, Shenzhen 518057, Peoples R China.
    Ahuja, Rajeev
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Materialteori.
    Ab Initio Screening of Doped Mg(AlH4)(2) Systems for Conversion-Type Lithium Storage2019Inngår i: Materials, ISSN 1996-1944, E-ISSN 1996-1944, Vol. 12, nr 16, artikkel-id 2599Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In this work, we have explored the potential applications of pure and various doped Mg(AlH4)(2) as Li-ion battery conversion electrode materials using density functional theory (DFT) calculations. Through the comparisons of the electrochemical specific capacity, the volume change, the average voltage, and the electronic bandgap, the Li-doped material is found to have a smaller bandgap and lower average voltage than the pure system. The theoretical specific capacity of the Li-doped material is 2547.64 mAhg(-1) with a volume change of 3.76% involving the electrode conversion reaction. The underlying reason for property improvement has been analyzed by calculating the electronic structures. The strong hybridization between Lis-state with H s-state influences the performance of the doped material. This theoretical research is proposed to help the design and modification of better light-metal hydride materials for Li-ion battery conversion electrode applications.

  • Liu, Xufeng
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Molekylär biomimetik.
    Miao, Rui
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Molekylär biomimetik.
    Lindberg, Pia
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Molekylär biomimetik.
    Lindblad, Peter
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Molekylär biomimetik.
    Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2 in cyanobacteria2019Inngår i: Energy & Environmental Science, ISSN 1754-5692, E-ISSN 1754-5706, Vol. 12, nr 9, s. 2765-2777Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Cyanobacteria are photoautotrophic microorganisms which can be engineered to directly convert CO2 and water into biofuels and chemicals via photosynthesis using sunlight as energy. However, the product titers and rates are the main challenges that need to be overcome for industrial applications. Here we present systematic modular engineering of the cyanobacterium Synechocystis PCC 6803, enabling efficient biosynthesis of 1-butanol, an attractive commodity chemical and gasoline substitute. Through introducing and re-casting the 1-butanol biosynthetic pathway at the gene and enzyme levels, optimizing the 5 '-regions of expression units for tuning transcription and translation, rewiring the carbon flux and rewriting the photosynthetic central carbon metabolism to enhance the precursor supply, and performing process development, we were able to reach a cumulative 1-butanol titer of 4.8 g L-1 with a maximal rate of 302 mg L-1 day(-1) from the engineered Synechocystis. This represents the highest 1-butanol production from CO2 reported so far. Our multi-level modular strategy for high-level production of chemicals and advanced biofuels represents a blue-print for future systematic engineering in photosynthetic microorganisms.

  • Oroujeni, Maryam
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Medicinsk strålningsvetenskap.
    Abouzayed, Ayman
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Theranostics.
    Lundmark, Fanny
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Preparativ läkemedelskemi.
    Mitran, Bogdan
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Theranostics.
    Orlova, Anna
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Theranostics. Uppsala universitet, Science for Life Laboratory, SciLifeLab.
    Tolmachev, Vladimir
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Medicinsk strålningsvetenskap.
    Rosenström, Ulrika
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Preparativ läkemedelskemi.
    Evaluation of Tumor-Targeting Properties of an Antagonistic Bombesin Analogue RM26 Conjugated with a Non-Residualizing Radioiodine Label Comparison with a Radiometal-Labelled Counterpart2019Inngår i: Pharmaceutics, ISSN 1999-4923, E-ISSN 1999-4923, Vol. 11, nr 8, artikkel-id 380Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Radiolabelled antagonistic bombesin analogues are successfully used for targeting of gastrin-releasing peptide receptors (GRPR) that are overexpressed in prostate cancer. Internalization of antagonistic bombesin analogues is slow. We hypothesized that the use of a non-residualizing radioiodine label might not affect the tumour uptake but would reduce the retention in normal organs, where radiopharmaceutical would be internalized. To test this hypothesis, tyrosine was conjugated via diethylene glycol linker to N-terminus of an antagonistic bombesin analogue RM26 to form Tyr-PEG(2)-RM26. [In-111]In-DOTA-PEG(2)-RM26 was used as a control with a residualizing label. Tyr-PEG(2)-RM26 was labelled with I-125 with 95% radiochemical purity and retained binding specificity to GRPR. The IC50 values for Tyr-PEG(2)-RM26 and DOTA-PEG(2)-RM26 were 1.7 +/- 0.3 nM and 3.3 +/- 0.5 nM, respectively. The cellular processing of [I-125]I-Tyr-PEG(2)-RM26 by PC-3 cells showed unusually fast internalization. Biodistribution showed that uptake in pancreas and tumour was GRPR-specific for both radioconjugates. Blood clearance of [I-125]I-Tyr-PEG(2)-RM26 was appreciably slower and activity accumulation in all organs was significantly higher than for [In-111]In-DOTA-PEG(2)-RM26. Tumor uptake of [In-111]In-DOTA-PEG(2)-RM26 was significantly higher than for [I-125]I-Tyr-PEG(2)-RM26, resulting in higher tumour-to-organ ratio for [In-111]In-DOTA-PEG(2)-RM26 at studied time points. Incorporation of amino acids with hydrophilic side-chains next to tyrosine might overcome the problems associated with the use of tyrosine as a prosthetic group for radioiodination.

  • Law, Jacob
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Matematisk-datavetenskapliga sektionen, Matematiska institutionen.
    Läroböckernas roll för gymnasieelevers begreppsförståelse inom trigonometri2019Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
  • Li, Jingguang
    et al.
    Dali Univ, Coll Educ, Dali, Peoples R China.
    Zhou, Xinlin
    Beijing Normal Univ, IDG McGovern Inst Brain Res, State Key Lab Cognit Neurosci & Learning, Beijing, Peoples R China.
    Lindskog, Marcus
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Institutionen för psykologi.
    Editorial: Approximate Number System and Mathematics2019Inngår i: Frontiers in Psychology, ISSN 1664-1078, E-ISSN 1664-1078, Vol. 10, artikkel-id 2084Artikkel i tidsskrift (Annet vitenskapelig)
  • Birkholzer, Jens T.
    et al.
    Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.
    Tsang, Chin-Fu
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Geovetenskapliga sektionen, Institutionen för geovetenskaper, Luft-, vatten och landskapslära. Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA.
    Bond, Alexander E.
    Quintessa Ltd, Birchwood, England.
    Hudson, John A.
    Imperial Coll London, London, England.
    Jing, Lanru
    KTH Royal Inst Technol, Stockholm, Sweden.
    Stephansson, Ove
    GFZ Helmholtz Ctr Geosci, Potsdam, Germany.
    25 years of DECOVALEX - Scientific advances and lessons learned from an international research collaboration in coupled subsurface processes2019Inngår i: International Journal of Rock Mechanics And Mining Sciences, ISSN 1365-1609, E-ISSN 1873-4545, Vol. 122, artikkel-id 103995Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    This paper provides an overview of an international research collaboration for advancing the understanding and modeling of coupled thermo-hydro-mechanical-chemical (THMC) processes in geological systems. The creation of the international DECOVALEX Project, now running for over 25 years, was initially motivated by the recognition that prediction of these coupled effects is an essential part of the performance and safety assessment of geologic disposal systems for radioactive waste and spent nuclear fuel. Later it was realized that these processes also play a critical role in other subsurface engineering activities, such as storage of CO2, exploration of enhanced geothermal systems, and unconventional oil and gas production through hydraulic fracturing. Research teams from radioactive waste management organizations, national research institutes, regulatory agencies, universities, as well as industry and consulting groups have participated in the DECOVALEX Project, providing a wide range of perspectives and solutions to these complex problems. Analysis and comparative modeling of state-of-the-art field and laboratory experiments has been at the core of the collaborative work, with an increasing focus on characterizing uncertainty and blind prediction of experimental results. Over these 25 years, many of the major advances in this field of research have been made through DECOVALEX, as evidenced by three books, seven journal special issues, and a good number of seminal papers that have emerged from the DECOVALEX modeling work. Examples of specific research advances will be presented in this paper to illustrate the significant impact of DECOVALEX on the current state-of-the-art of understanding and modeling coupled THMC processes. These examples range from the modeling of large-scale in situ heater tests representing mock-ups of nuclear waste disposal tunnels, to studies of fluid flow and chemical-mechanical coupling in heterogeneous fractures, and to the numerical analysis of controlled-injection meso-scale fault slip experiments.

  • Cedervall, Johan
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Oorganisk kemi.
    Ivanov, Sergey A.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper. Karpov Inst Phys Chem, Ctr Mat Sci, Vorontsovo Pole 10, Moscow 105064, Russia;Uppsala Univ, Dept Engn Sci, Box 534, S-75121 Uppsala, Sweden.
    Lewin, Erik
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Oorganisk kemi. Uppsala Univ, Dept Chem, Angstrom Lab, Box 538, S-75121 Uppsala, Sweden.
    Beran, Premysl
    ESS, Tunavagen 24, S-22363 Lund, Sweden;Acad Sci Czech Republ, Nucl Phys Inst, Rez 25068, Czech Republic.
    Andersson, Mikael S.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper.
    Faske, Tom
    Tech Univ Darmstadt, Inst Mat & Geowissensch, Alarich Weiss Str 2, D-64287 Darmstadt, Germany.
    Bazuev, Gennadii V.
    Russian Acad Sci, Inst Solid State Chem, Ural Branch, Ekaterinburg 620990, Russia.
    Nordblad, Per
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets fysik.
    Sahlberg, Martin
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Oorganisk kemi.
    Mathieu, Roland
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets fysik.
    On the structural and magnetic properties of the double perovskite Nd2NiMnO62019Inngår i: Journal of materials science. Materials in electronics, ISSN 0957-4522, E-ISSN 1573-482X, Vol. 30, nr 17, s. 16571-16578Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The structural, electronic and magnetic properties of phase pure and stoichiometric samples of the double perovskite Nd2NiMnO6. Photoectron spectroscopy revels a mixed valence of the transition metal sites where Ni has 3+/2+ oxidation states and Mn has 3+/4+. The compound orders ferromagnetically at 195 K. The magnetic structure was determined from the refinement of the neutron diffraction data. The results suggests that the B-site magnetic moments align along the crystallographic a-direction.

  • Arce, Maximiliano
    et al.
    Pontificia Univ Catolica Chile, Fac Biol Sci, Santiago 8331150, Chile;Adv Ctr Chron Dis ACCDiS, Santiago, Chile.
    Pinto, Mauricio P.
    Pontificia Univ Catolica Chile, Fac Med, Santiago 8331150, Chile.
    Galleguillos, Macarena
    Pontificia Univ Catolica Chile, Fac Biol Sci, Santiago 8331150, Chile.
    Munoz, Catalina
    Pontificia Univ Catolica Chile, Fac Biol Sci, Santiago 8331150, Chile.
    Lange, Soledad
    Pontificia Univ Catolica Chile, Fac Biol Sci, Santiago 8331150, Chile.
    Ramirez, Carolina
    Pontificia Univ Catolica Chile, Fac Biol Sci, Santiago 8331150, Chile.
    Erices, Rafaela
    Pontificia Univ Catolica Chile, Fac Biol Sci, Santiago 8331150, Chile;Univ Mayor, Vicerrectoria Invest, Santiago 7510041, Chile.
    Gonzalez, Pamela
    Pontificia Univ Catolica Chile, Fac Biol Sci, Santiago 8331150, Chile.
    Velasquez, Ethel
    Pontificia Univ Catolica Chile, Fac Biol Sci, Santiago 8331150, Chile;Comis Chilena Energia Nucl CCHEN, Santiago, Chile.
    Tempio, Fabian
    Univ Chile, Fac Med, Inst Biomed Sci, Santiago 8380453, Chile.
    Lopez, Mercedes N.
    Univ Chile, Fac Med, Inst Biomed Sci, Santiago 8380453, Chile;Millennium Inst Immunol & Immunotherapy, Santiago 8331150, Chile.
    Salazar-Onfray, Flavio
    Univ Chile, Fac Med, Inst Biomed Sci, Santiago 8380453, Chile;Millennium Inst Immunol & Immunotherapy, Santiago 8331150, Chile.
    Cautivo, Kelly
    Pontificia Univ Catolica Chile, Fac Biol Sci, Santiago 8331150, Chile.
    Kalergis, Alexis M.
    Pontificia Univ Catolica Chile, Fac Biol Sci, Santiago 8331150, Chile;Millennium Inst Immunol & Immunotherapy, Santiago 8331150, Chile;Biomed Res Consortium Chile, Santiago 8331010, Chile.
    Cruz, Sebastian
    Fdn Ciencia & Vida, Lab Immunoncol, Santiago, Chile.
    Lladser, Alvaro
    Millennium Inst Immunol & Immunotherapy, Santiago 8331150, Chile;Fdn Ciencia & Vida, Lab Immunoncol, Santiago, Chile.
    Lobos-Gonzalez, Lorena
    Adv Ctr Chron Dis ACCDiS, Santiago, Chile;Fdn Ciencia & Vida, Lab Immunoncol, Santiago, Chile;Univ Desarrollo, Fac Med, Regenerat Med Ctr, Clin Alemana, Santiago 7650568, Chile.
    Valenzuela, Guillermo
    Pontificia Univ Catolica Chile, Fac Med, Santiago 8331150, Chile.
    Olivares, Nixa
    Pontificia Univ Catolica Chile, Fac Med, Santiago 8331150, Chile.
    Saez, Claudia
    Pontificia Univ Catolica Chile, Fac Med, Santiago 8331150, Chile.
    Koning, Tania
    Univ Austral Chile, Fac Med, Immunol Inst, Valdivia 5110566, Chile.
    Sanchez, Fabiola A.
    Univ Austral Chile, Fac Med, Immunol Inst, Valdivia 5110566, Chile.
    Fuenzalida, Patricia
    Pontificia Univ Catolica Chile, Fac Biol Sci, Santiago 8331150, Chile.
    Godoy, Alejandro
    Pontificia Univ Catolica Chile, Fac Biol Sci, Santiago 8331150, Chile;Roswell Pk Comprehens Canc Ctr, Dept Urol, Buffalo, NY 14203 USA.
    Contreras Orellana, Pamela
    Adv Ctr Chron Dis ACCDiS, Santiago, Chile;Univ Chile, Fac Med, Lab Cellular Commun, ICBM, Santiago 8380453, Chile.
    Leyton, Lisette
    Adv Ctr Chron Dis ACCDiS, Santiago, Chile;Univ Chile, Fac Med, Lab Cellular Commun, ICBM, Santiago 8380453, Chile.
    Lugano, Roberta
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Vaskulärbiologi.
    Dimberg, Anna
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Vaskulärbiologi.
    Quest, Andrew F. G.
    Adv Ctr Chron Dis ACCDiS, Santiago, Chile;Univ Chile, Fac Med, Lab Cellular Commun, ICBM, Santiago 8380453, Chile.
    Owen, Gareth, I
    Pontificia Univ Catolica Chile, Fac Biol Sci, Santiago 8331150, Chile;Adv Ctr Chron Dis ACCDiS, Santiago, Chile;Pontificia Univ Catolica Chile, Fac Med, Santiago 8331150, Chile;Millennium Inst Immunol & Immunotherapy, Santiago 8331150, Chile.
    Coagulation Factor Xa Promotes Solid Tumor Growth, Experimental Metastasis and Endothelial Cell Activation2019Inngår i: Cancers, ISSN 2072-6694, Vol. 11, nr 8, artikkel-id 1103Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Hypercoagulable state is linked to cancer progression; however, the precise role of the coagulation cascade is poorly described. Herein, we examined the contribution of a hypercoagulative state through the administration of intravenous Coagulation Factor Xa (FXa), on the growth of solid human tumors and the experimental metastasis of the B16F10 melanoma in mouse models. FXa increased solid tumor volume and lung, liver, kidney and lymph node metastasis of tail-vein injected B16F10 cells. Concentrating on the metastasis model, upon coadministration of the anticoagulant Dalteparin, lung metastasis was significantly reduced, and no metastasis was observed in other organs. FXa did not directly alter proliferation, migration or invasion of cancer cells in vitro. Alternatively, FXa upon endothelial cells promoted cytoskeleton contraction, disrupted membrane VE-Cadherin pattern, heightened endothelial-hyperpermeability, increased inflammatory adhesion molecules and enhanced B16F10 adhesion under flow conditions. Microarray analysis of endothelial cells treated with FXa demonstrated elevated expression of inflammatory transcripts. Accordingly, FXa treatment increased immune cell infiltration in mouse lungs, an effect reduced by dalteparin. Taken together, our results suggest that FXa increases B16F10 metastasis via endothelial cell activation and enhanced cancer cell-endothelium adhesion advocating that the coagulation system is not merely a bystander in the process of cancer metastasis.

  • Hindersson, Kajsa
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Sociologiska institutionen.
    Barnarbete - ett resultat av brister inom socialt arbete?: En litteraturstudie om det globala barnarbetet2019Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Denna uppsats är en systematisk litteraturstudie, vilken behandlar ämnet barnarbete utifrån ett globalt perspektiv. Hur ser barnarbetet ut i dess mest omfattande platser – i Afrika, Asien och Latinamerika? Hur ser det sociala arbetet ut där? Samt hur kan vi förstå sambandet mellan barnarbete och brister inom socialt arbete som en orsak till problemets kvarlevande? Studien syftar till att sammanställa det aktuella kunskapsläget om arbetande barn i de områden som ratificerat barnkonventionen, men där barnarbete trots det anses vara som mest skadligt och därav talas det ofta om den värsta typen av barnarbete i denna studie. Studien visar även en problematik gällande dessa områdens utveckling av socialpolitik samt det sociala arbetet och resultatet analyseras utifrån den marxistiska samhällsteorin. Resultatet och analysen visar att barnarbetet många gånger skiljer sig åt från land till land och från område till område, men också vilka likheter som råder oberoende plats. Exempelvis belys barnarbetarnas hälsotillstånd som någonting likartat världen över och därmed barnarbetets konsekvenser. Även om barnarbete är ett komplext problem där ett flertal olika faktorer spelar roll och bör förstås utifrån sin kontext visar slutligen analysen, med hjälp av den marxistiska samhällsteorin, att vi kan förstå de aktuella områdenas brist av socialt arbete, socialpolitik och sociala insatser som en del i varför barnarbete fortfarande finns kvar.

  • Ding, Haozhong
    et al.
    KTH Royal Inst Technol, Dept Prot Sci, Roslagstullsbacken 21, S-11417 Stockholm, Sweden.
    Altai, Mohamed
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Medicinsk strålningsvetenskap.
    Rinne, Sara S.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Theranostics.
    Vorobyeva, Anzhelika
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Medicinsk strålningsvetenskap.
    Tolmachev, Vladimir
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Medicinsk strålningsvetenskap.
    Gräslund, Torbjorn
    KTH Royal Inst Technol, Dept Prot Sci, Roslagstullsbacken 21, S-11417 Stockholm, Sweden.
    Orlova, Anna
    Uppsala universitet, Science for Life Laboratory, SciLifeLab. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Theranostics.
    Incorporation of a Hydrophilic Spacer Reduces Hepatic Uptake of HER2-Targeting Affibody-DM1 Drug Conjugates2019Inngår i: Cancers, ISSN 2072-6694, Vol. 11, nr 8, artikkel-id 1168Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Affibody molecules are small affinity-engineered scaffold proteins which can be engineered to bind to desired targets. The therapeutic potential of using an affibody molecule targeting HER2, fused to an albumin-binding domain (ABD) and conjugated with the cytotoxic maytansine derivate MC-DM1 (AffiDC), has been validated. Biodistribution studies in mice revealed an elevated hepatic uptake of the AffiDC, but histopathological examination of livers showed no major signs of toxicity. However, previous clinical experience with antibody drug conjugates have revealed a moderateto high-grade hepatotoxicity in treated patients, which merits efforts to also minimize hepatic uptake of the AffiDCs. In this study, the aim was to reduce the hepatic uptake of AffiDCs and optimize their in vivo targeting properties. We have investigated if incorporation of hydrophilic glutamate-based spacers adjacent to MC-DM1 in the AffiDC, (Z(HER2:2891))(2) -ABD-MC-DM1, would counteract the hydrophobic nature of MC-DM1 and, hence, reduce hepatic uptake. Two new AffiDCs including either a triglutamate-spacer-, (Z(HER2:2891))(2)-ABD-E-3-MC-DM1, or a hexaglutamate-spacer-, (Z(HER2:2891))(2)-ABD-E-6-MC-DM1 next to the site of MC-DM1 conjugation were designed. We radiolabeled the hydrophilized AffiDCs and compared them, both in vitro and in vivo, with the previously investigated (Z(HER2:2891))(2)-ABD-MC-DM1 drug conjugate containing no glutamate spacer. All three AffiDCs demonstrated specific binding to HER2 and comparable in vitro cytotoxicity. A comparative biodistribution study of the three radiolabeled AffiDCs showed that the addition of glutamates reduced drug accumulation in the liver while preserving the tumor uptake. These results confirmed the relation between DM1 hydrophobicity and liver accumulation. We believe that the drug development approach described here may also be useful for other affinity protein-based drug conjugates to further improve their in vivo properties and facilitate their clinical translatability.

  • Meyer-Lie, Tove
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Åsenlöf: Fysioterapi.
    Violiniststudenters upplevelser och erfarenheter av att spela med smärta eller skada: En kvalitativ studie2019Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Bakgrund

    Det är välkänt att musiker, studerande såväl som praktiserande, ofta drabbas av smärtproblematik och det finns en hög prevalens för smärta i nacke, skuldra, arm och handområden hos violinister.

     

    Syfte

    Syftet var att belysa och beskriva violiniststudenters upplevelser av att spela med smärtor/skador och upplevelser om underlättande och försvårande faktorer för spel samt deras upplevelser om behandlingar/möjlighet till behandlingar.

     

    Design och metod

    Studien har en kvalitativ ansats med en explorativ design med semistrukturerade intervjuer som datainsamlingsmetod.

     

    Resultat

    Ur analysen identifierades fem kategorier: ”Konsekvenser av smärta och skada vid spel”, ”smärtans påverkan i vardagen”, ”underlättande faktorer för spel”, ”försvårande faktorer för spel”, och ”upplevelser av behandling”. God kunskap hos musikläraren, ergonomi och fysisk träning upplevdes som underlättande faktorer och minskade smärtan. Instrumentets utformning och höga krav upplevdes som försvårande faktorer för spel och påverkade smärtproblematiken negativt. Deras upplevelser av behandling var att vården var oförstående för deras problematik och att mer kunskap om hur de kan få hjälp behövs.

     

    Konklusion

    Fysisk träning, ergonomi och coping är exempel på faktorer som underlättat för spel och minskat smärtproblematiken hos studenterna. Detta kan vården och specifikt fysioterapeuter hjälpta till med men det behöver utforskas ytterligare för att kunna utveckla anpassade behandlingar för att minska smärtproblematik hos violiniststudenter.  

  • Sandberg, Leo
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Historisk-filosofiska fakulteten, Institutionen för speldesign. Konstfack.
    Visual interpretation: Intent and response2013Independent thesis Advanced level (professional degree), 10 poäng / 15 hpOppgave
    Abstract [en]

    This paper explores artistic interpretation of a script's theme to its visual, estetic representation and meaning. The purpose is to reflect on the topic, and to enhance our understanding of how an interpretation from written intention to visual representation can form. The aritstic production used in this artistic research is an animated feature film for children 10+ and the character design of its lead female character. 

  • Ströberg, Simon
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Historisk-filosofiska fakulteten, Institutionen för speldesign.
    ADHD Through the Lens of Game Design: How Digital RPGs Neutralize the Symptoms of Inattention Amongst Swedish Adults with ADHD2018Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [en]

    ADHD (Attention Deficit Hyperactivity Disorder) is a commonly diagnosed mental disorder with an estimated global prevalence of 5.29% that exhibit inattentive, hyperactive and impulsive behaviors, many of which can negatively impact an individual’s social, academic, occupational and everyday-life. Studies based on the Delay Aversion Hypothesis have shown that video-games effectively contribute to the neutralization of some of the inattentive symptoms of ADHD, and that games could act as a possible treatment option for individuals with ADHD. Attempts to use video games as a treatment option has previously been tried, however to a limited extent. This study approaches the topic from the game design perspective, and discusses which game mechanics, activities and stimuli contribute to the possible neutralization of the inattentive symptoms of ADHD, in order to lay a foundation for future research within the area. The study found that the participants with ADHD acted in ways that contradicted some of the described symptoms of ADHD in the DSM-5 (Diagnostic and Statistical Manual of Mental Disorders) when playing video games, which could be attributed to the amount of stimuli available in video games. Additionally, the participants’ level of motivation when playing video games seemed to be strongly correlated to the principles of andragogy, which could indicate that children with ADHD might benefit from an educational system that combines and incorporates principles from both andragogy and pedagogy.

  • Wolfram, Jakub
    et al.
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Historisk-filosofiska fakulteten, Institutionen för speldesign.
    Näsberg, Rebecka
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Historisk-filosofiska fakulteten, Institutionen för speldesign.
    In what ways do game design students perceive microtransactions in free-to-play online PC games?2019Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [en]

    In recent years, microtransactions have become a norm for monetization and a standardbusiness model in free-to-play games. This is something that affects multiple individuals andhas caused quite a stir among players. Through this research, we want to gain anunderstanding of why some microtransaction types might be considered more acceptable thanothers. In addition, to gaining potential data that could possibly give us a look into the futureof the design of microtransactions. We have gathered our data by conducting online chatbased semi-structured interviews with current and former university students studying gamedesign at Uppsala University.The research found that game design students have a predominantly negative opinion aboutmost microtransactions, especially when they were affecting the gameplay, contrary to whenthey were not.

  • Levinsson, Ellinore
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Historisk-filosofiska fakulteten, Litteraturvetenskapliga institutionen.
    Identitet och klass i svensk modern arbetarlitteratur: En närläsning av Jenny Wrangborg, Sara Beischer och Elise Karlsson2018Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • Tan, Jun Liang
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Geovetenskapliga sektionen, Institutionen för geovetenskaper.
    Development of a pitch based wake optimisation control strategy to improve total farm power production2016Independent thesis Advanced level (degree of Master (One Year)), 10 poäng / 15 hpOppgave
    Abstract [en]

    In this thesis, the effect of pitch based optimisation was explored for a 80 turbine wind farm. Using a modified Jensen wake model and the Particle Swarm Optimisation (PSO) model, a pitch optimisation strategy was created for the dominant turbulence and atmospheric condition for the wind farm.

    As the wake model was based on the FLORIS model developed by P.M.O Gebraad et. al., the wake and power model was compared with the FLORIS model and a -0.090% difference was found. To determine the dynamic predictive capability of the wake model, measurement values across a 10 minute period for a 19 wind turbine array were used and the wake model under predicted the power production by 17.55%. Despite its poor dynamic predictive capability, the wake model was shown to accurately match the AEP production of the wind farm when compared to a CFD simulation done in FarmFlow and only gave a 3.10% over-prediction.

    When the optimisation model was applied with 150 iterations and particles, the AEP production of the wind farm increased by 0.1052%, proving that the pitch optimisation method works for the examined wind farm. When the iterations and particles used for the optimisation was increased to 250, the power improvement between optimised results improved by 0.1144% at a 222.5% increase in computational time, suggesting that the solution has yet to fully converge. While the solutions did not fully converge, they converged sufficiently and an increase in iterations gave diminishing results. From the results, the pitch optimisation model was found to give a significant increase in power production, especially in wake intensive wind directions. However, the dynamic predictive capabilities will have be improved upon before the control strategy can be applied to an operational wind farm.

  • Klemm, Maria
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Geovetenskapliga sektionen, Institutionen för geovetenskaper.
    Economic Feasibility of Wind Turbine's Anti-Icing Systems Development and application of a simple heuristic method and tool2014Independent thesis Advanced level (degree of Master (One Year)), 10 poäng / 15 hpOppgave
    Abstract [en]

    Wind energy projects in cold climate areas are more and more favoured due to the presence of high wind potential and the availability of land in those areas. A serious problem, however, is the impact of icing on the wind turbine and on the energy production.

    The main objective of this Thesis is to present the available options for mitigating the risks of wind turbines’ blade icing and to evaluate the investment of the wind turbines’ anti-icing systems. To this end, a heuristic algorithmic method has been developed as well as a simple computational tool in order to assess the economic feasibility of anti-icing systems. The method has subsequently been applied in three case studies and relevant conclusions have been drawn

    Initially a literature review has been conducted that encompassed issues like the direct and indirect impacts of icing on wind turbines (reduced yield, increased loads and noise emission, health and safety risks, etc.), the difficulties of estimating icing formulation and icing production losses and the anti-icing and de-icing systems and their functionality, advantages and disadvantages.

    Subsequently, the heuristic algorithmic method has been developed which is based on estimating the energy production of a wind turbine for three scenarios - with no icing occurring, with icing but without any anti- and de-icing system installed and with an anti-icing system installed – followed by a sensitivity analysis of the economic performance parameter, the simple pay-back period.

    The method has been accommodated in a simple computational excel-based tool and has been applied to three case studies located in the north Norway, in central Sweden and in south Finland. The production losses related to icing are in the expected ranges typical for the geographic location and so is the economic performance parameter which indicate that the investment in an anti-icing system is recommended for the three case studies. Results showed that the wind resources, the electricity export price and the annual icing hours are the parameters that determine the feasibility of an anti-icing system for a wind farm project.

    Wind energy projects in cold climate areas are more and more favoured due to the presence of high wind potential and the availability of land in those areas. A serious problem, however, is the impact of icing on the wind turbine and on the energy production.

    The main objective of this Thesis is to present the available options for mitigating the risks of wind turbines’ blade icing and to evaluate the investment of the wind turbines’ anti-icing systems. To this end, a heuristic algorithmic method has been developed as well as a simple computational tool in order to assess the economic feasibility of anti-icing systems. The method has subsequently been applied in three case studies and relevant conclusions have been drawn

    Initially a literature review has been conducted that encompassed issues like the direct and indirect impacts of icing on wind turbines (reduced yield, increased loads and noise emission, health and safety risks, etc.), the difficulties of estimating icing formulation and icing production losses and the anti-icing and de-icing systems and their functionality, advantages and disadvantages.

    Subsequently, the heuristic algorithmic method has been developed which is based on estimating the energy production of a wind turbine for three scenarios - with no icing occurring, with icing but without any anti- and de-icing system installed and with an anti-icing system installed – followed by a sensitivity analysis of the economic performance parameter, the simple pay-back period.

    The method has been accommodated in a simple computational excel-based tool and has been applied to three case studies located in the north Norway, in central Sweden and in south Finland. The production losses related to icing are in the expected ranges typical for the geographic location and so is the economic performance parameter which indicate that the investment in an anti-icing system is recommended for the three case studies. Results showed that the wind resources, the electricity export price and the annual icing hours are the parameters that determine the feasibility of an anti-icing system for a wind farm project.

  • Alvarez, Manuel
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Geovetenskapliga sektionen, Institutionen för geovetenskaper.
    Improving the modelling of marine operations in the installation of offshore wind farms2016Independent thesis Advanced level (degree of Master (One Year)), 10 poäng / 15 hpOppgave
  • Bergvall, Daniel
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Geovetenskapliga sektionen, Institutionen för geovetenskaper.
    Cost Comparison of Repowering Alternatives for Offshore Wind Farms2019Independent thesis Advanced level (degree of Master (One Year)), 10 poäng / 15 hpOppgave
    Abstract [en]

    The aim of this thesis is to evaluate different repowering alternatives from the viewpoint of increasing power production from existing offshore wind farms (OWF), as some of the first commissioned OWFs are approaching the end of their expected lifetime. The thesis presents a literature review of components and financial aspects that are of importance for repowering of OWFs. In the literature review, risks and uncertainties regarding repowering are also lifted and analysed. The thesis contains a case study on Horns Rev 1 OWF, where three different repowering scenarios are evaluated by technical and financial performance, aiming to compare the cost of repowering alternatives. The design of the case study is based around previous studies of offshore repowering having focused mainly on achieving the lowest possible levelized cost of energy (LCoE) and highest possible capacity factor, often resulting in suggested repowering utilizing smaller wind turbines than the existing ones.

    In order to evaluate the financial viability of repowering alternatives, the software RETScreen Expert was used to estimate the annual energy production (AEP) after losses and calculate the net present value (NPV) and LCoE for lifetime extension and full repowering utilizing different capacity wind turbines. Input values from the literature as well as real wind resource measurements from the site was utilized to achieve as accurate results as possible.

    The result of the case study shows that repowering of OWFs have the possibility of providing a very strong business case with all scenarios resulting in a positive NPV as well as lower LCoE than the benchmarked electricity production price. Although the initial investment cost of the different repowering alternatives presented in this thesis still are uncertain to some extent, due to the lack of reliable costs for repowering alternatives, this thesis provides a base for further research regarding the repowering of OWFs.

  • Pettersson, Erica
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Språkvetenskapliga fakulteten, Institutionen för nordiska språk.
    Nybyggarmål i ny tid: En diskursanalytisk studie av norrbottniska dialekters beskrivningar i tre läroböcker2019Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    I denna uppsats kartläggs och analyseras beskrivningar av norrbottniska dialekter som presenteras i tre inflytelserika läromedel i dialektologi. Detta har gjorts i syfte att undersöka hur beskrivningarna kan kopplas till begreppet dialekt samt huruvida beskrivningarna kan relateras till identitetsskapandet hos talare av norrbottniska dialekter.

    Materialet utgörs av Elias Wesséns Våra folkmål (1969), Bengt Pamps Svenska dialekter (1978) och Gertrud Petterssons Svenska språket under sjuhundra år (2005). Uppsatsen använder sig av den diskursanalytiska nivån dispositiv. Genom metoden kontrasteras materialets beskrivningar av språket i södra respektive norra Norrbotten.

    Resultatet visar att materialets beskrivningar av språket i norra Norrbotten är ytliga och i vissa fall icke-existerande. Studiens slutsats är att materialet reproducerar en dialektologiskt traditionell bild av norrbottniskt språk, som inte stämmer överens med områdets faktiska språkförhållanden. Till sist visar studien även på ett behov av ny forskning om språket i norra Norrbotten.

  • Szkalej, Kacper
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Juridiska fakulteten, Juridiska institutionen.
    Enabling Access to Digital Content2019Inngår i: EU Copyright Law: Subsistence, Exploitation and Protection of Rights, Alphen aan den Rijn: Kluwer Law International, 2019, s. 81-106Kapittel i bok, del av antologi (Fagfellevurdert)
    Abstract [en]

    The paper describes and critically evaluates the Cross-Border Portability Regulation (2017/1128) with reference to its scope and intended purpose. The paper shows that although the Regulation can be regarded as a first of its kind consumer copyright law-type of instrument taking as its point of departure difficulties with accessing legal content, the Regulation, having been hastily adopted in the shadow of negotiations preceding the adoption of the DSM Directive, leaves a number of crucial issues unanswered, such as whether it in fact applies to other types of access to digital content than subscription-based access such as streaming services. The paper is a contribution to EU Copyright Law: Subsistence, Exploitation and Protection of Rights (Kluwer Law International, 2019) intended to be accessible to a wider audience and is based on the author's ongoing research.

  • Disputas: 2019-11-08 10:15 2446, Uppsala
    Kruchinina, Anastasia
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Matematisk-datavetenskapliga sektionen, Institutionen för informationsteknologi, Avdelningen för beräkningsvetenskap. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Matematisk-datavetenskapliga sektionen, Institutionen för informationsteknologi, Tillämpad beräkningsvetenskap.
    Efficient Density Matrix Methods for Large Scale Electronic Structure Calculations2019Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    Efficient and accurate methods for computing the density matrix are necessary to be able to perform large scale electronic structure calculations. For sufficiently sparse matrices, the computational cost of recursive polynomial expansions to construct the density matrix scales linearly with increasing system size. In this work, parameterless stopping criteria for recursive polynomial expansions are developed. The proposed stopping criteria automatically adapt to a change in the requested accuracy, perform at almost no additional cost and do not require any user-defined tolerances.

    Compared to the traditional diagonalization approach, in linear scaling methods molecular orbitals are not readily available. In this work, the interior eigenvalue problem for the Fock/Kohn-Sham matrix is coupled to the recursive polynomial expansions. The idea is to view the polynomial, obtained in the recursive expansion, as an eigenvalue filter, giving large separation between eigenvalues of interest. An efficient method for computation of homo and lumo eigenvectors is developed. Moreover, a method for computation of multiple eigenvectors around the homo-lumo gap is implemented and evaluated.

    An original method for inverse factorization of Hermitian positive definite matrices is developed in this work. Novel theoretical tools for analysis of the decay properties of matrix element magnitude in electronic structure calculations are proposed. Of particular interest is an inverse factor of the basis set overlap matrix required for the density matrix construction. It is shown that the proposed inverse factorization algorithm drastically reduces the communication cost compared to state-of-the-art methods.

    To perform large scale numerical tests, most of the proposed methods are implemented in the quantum chemistry program Ergo, also presented in this thesis. The recursive polynomial expansion in Ergo is parallelized using the Chunks and Tasks matrix library. It is shown that the communication cost per process of the recursive polynomial expansion implementation tends to a constant in a weak scaling setting.

    Delarbeid
    1. Parameterless stopping criteria for recursive density matrix expansions
    Åpne denne publikasjonen i ny fane eller vindu >>Parameterless stopping criteria for recursive density matrix expansions
    2016 (engelsk)Inngår i: Journal of Chemical Theory and Computation, ISSN 1549-9618, E-ISSN 1549-9626, Vol. 12, s. 5788-5802Artikkel i tidsskrift (Fagfellevurdert) Published
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-310710 (URN)10.1021/acs.jctc.6b00626 (DOI)000389866500011 ()
    Prosjekter
    eSSENCE
    Tilgjengelig fra: 2016-10-26 Laget: 2016-12-19 Sist oppdatert: 2019-09-20bibliografisk kontrollert
    2. On-the-fly computation of frontal orbitals in density matrix expansions
    Åpne denne publikasjonen i ny fane eller vindu >>On-the-fly computation of frontal orbitals in density matrix expansions
    2018 (engelsk)Inngår i: Journal of Chemical Theory and Computation, ISSN 1549-9618, E-ISSN 1549-9626, Vol. 14, s. 139-153Artikkel i tidsskrift (Fagfellevurdert) Published
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-338750 (URN)10.1021/acs.jctc.7b00968 (DOI)000419998300013 ()29193971 (PubMedID)
    Prosjekter
    eSSENCE
    Tilgjengelig fra: 2017-12-01 Laget: 2018-01-12 Sist oppdatert: 2019-09-20bibliografisk kontrollert
    3. Ergo: An open-source program for linear-scaling electronic structure calculations
    Åpne denne publikasjonen i ny fane eller vindu >>Ergo: An open-source program for linear-scaling electronic structure calculations
    2018 (engelsk)Inngår i: SoftwareX, E-ISSN 2352-7110, Vol. 7, s. 107-111Artikkel i tidsskrift (Fagfellevurdert) Published
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-348090 (URN)10.1016/j.softx.2018.03.005 (DOI)000457139300020 ()
    Prosjekter
    eSSENCE
    Tilgjengelig fra: 2018-04-05 Laget: 2018-04-10 Sist oppdatert: 2019-09-20bibliografisk kontrollert
    4. Localized inverse factorization
    Åpne denne publikasjonen i ny fane eller vindu >>Localized inverse factorization
    2018 (engelsk)Inngår i: Computing Research Repository, nr 1812.04919Artikkel i tidsskrift (Annet vitenskapelig) Submitted
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-381327 (URN)
    Prosjekter
    eSSENCE
    Tilgjengelig fra: 2018-12-12 Laget: 2019-04-08 Sist oppdatert: 2019-09-20bibliografisk kontrollert
    5. Efficient computation of the density matrix with error control on distributed computer systems
    Åpne denne publikasjonen i ny fane eller vindu >>Efficient computation of the density matrix with error control on distributed computer systems
    2019 (engelsk)Inngår i: Computing Research Repository, nr 1909.12533Artikkel i tidsskrift (Annet vitenskapelig) Submitted
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-393386 (URN)
    Prosjekter
    eSSENCE
    Tilgjengelig fra: 2019-09-27 Laget: 2019-09-20 Sist oppdatert: 2019-09-30bibliografisk kontrollert
    6. Multiple eigenvectors around the homo–lumo gap as a cheap by-product in linear scaling electronic structure calculations
    Åpne denne publikasjonen i ny fane eller vindu >>Multiple eigenvectors around the homo–lumo gap as a cheap by-product in linear scaling electronic structure calculations
    2019 (engelsk)Inngår i: Computing Research Repository, nr 1909.11662Artikkel i tidsskrift (Annet vitenskapelig) Submitted
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-393385 (URN)
    Prosjekter
    eSSENCE
    Tilgjengelig fra: 2019-09-25 Laget: 2019-09-20 Sist oppdatert: 2019-09-30bibliografisk kontrollert
  • Nabseth, Jonas
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Matematisk-datavetenskapliga sektionen, Institutionen för informationsteknologi.
    Detecting Anomalies in User Communication in an E-commerce Application: Applying a Clustering Algorithm to VectorizedText Messages2019Independent thesis Advanced level (professional degree), 20 poäng / 30 hpOppgave
    Abstract [en]

    Many applications that allow text based communication between users are troubled with malicious content. This thesis presents a system for detecting such behaviour in an E-commerce application. The system is based on an algorithm for anomaly detection which is trained using messages sent between users in the application. Preprocessing of the text is performed using the NLP-toolbox Glove. The resulting word embeddings are used to create numerical representations of messages, which are then used as input to a clustering algorithm based on K-means. Vectors positioned far away from existing clusters were considered anomalies. This report assesses performance of this system, and relates this to the performance achieved with an existing approach.

  • Lopez Gomez Partida, Fausto
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Elektricitetslära.
    Design of a Permanent Magnet Synchronous Generator with Alnico Magnets2019Independent thesis Advanced level (professional degree), 20 poäng / 30 hpOppgave
    Abstract [en]

    Following the trends to diminish the fossil fuel energy production new technologies known for their renewable sources have become a signficant option for helping combat climate change and handle the current oil prices. These new technologies base their power production on already established physical principles that convert mechanical power to electrical power.

    Generators are the fundamental piece of machinery for electricity production. Among the various types of generators that exist, permanent magnet synchronous generators (PMSGs) are commonly used for renewable electricity production. At present, the most used magnets for PMSGs are alloys of neodymium, iron, and boron which form a tetragonal crystalline structure known as Neodymium magnets (NdFeB). These types of magnets contain rare-earth materials, which makes them highly non-sustainable materials. Research to find new magnet compositions to substitute rare earth magnets or to reduce the weight and increase the efficiency of PMSGs is currently being studied. One option is to use Alnico magnets. This thesis project explores this option.

    With the help of a finite element analysis (FEA) software (COMSOL Multiphysics), three types of Alnico grades 5, 8 and 9 were implemented in the rotor of a spoke type generator to study the load limits of the rotor magnets, and together with this observe the demagnetization and impact that it has on the power production of the generator, in two different scenarios: 1) When the generator is connected to a nominal load under normal conditions and 2) when the generator is connected to a nominal load after a short circuit (SC).

    The simulations provided an insight into the load limitations that the generator has by each type of Alnico studied. Alnico 9 showed to be the best candidate magnet from the three magnets implemented with less demagnetization and higher electrical power output, followed by Alnico 8, which presented a good electrical power output at the nominal load scenario. Regardless of the higher demagnetization of Alnico 5, it proved to be a better candidate than Alnico 8 at the SC scenario.

  • Arman Vatandoust, Arman Vatandoust
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Matematisk-datavetenskapliga sektionen, Institutionen för informationsteknologi.
    Machine Learning for Software Bug Categorization2019Independent thesis Advanced level (professional degree), 20 poäng / 30 hpOppgave
    Abstract [en]

    The pursuit of flawless software is often an exhausting task for software developers. Code defects can range from soft issues to hard issues that lead to unforgiving consequences. DICE have their own system which automatically collects these defects which are grouped into buckets, however, this system suffers from the flaw of sometimes incorrectly grouping unrelated issues, and missing apparent duplicates. This time-consuming flaw puts excessive work for software developers and leads to wasted resources in the company. These flaws also impact the data quality of the system's defects tracking datasets which turn into a never-ending vicious circle. In this thesis, we investigate the method of measuring the similarity between reports in order to reduce incorrectly grouped issues and duplicate reports. Prototype models have been built for bug categorization and bucketing using convolutional neural networks. For each report, the prototype is able to provide developers with candidates of related issues with likelihood metric whether the issues are related. The similarity measurement is made in the representation phase of the neural networks, which we call the latent space. We also use Kullback–Leibler divergence in this space in order to get better similarity metrics. The results show important findings and insights for further improvement in the future. In addition to this, we discuss methods and strategies for detecting outliers using Mahalanobis distance in order to prevent incorrectly grouped reports.

  • Disputas: 2019-11-08 13:15 Häggsalen, Uppsala
    Öberg, Viktor A.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Quantum Dot Solar Cells: Towards Environmentally Friendly Materials2019Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    To decrease the world’s energy dependence on fossil fuels, energy production and specifically electricity production needs to shift to more sustainable alternatives. One such alternative is solar cells as they directly convert energy emitted from the sun into electricity. Silicon and thin film solar cells which are commercialized today are either expensive to make and rigid, or utilizes rare and toxic materials. This has resulted in an increase in the field of solar cell research to find cheaper alternatives which are also based on abundant materials.

    Colloidal quantum dot (CQD) solar cells are third generation solar cells which has received much focus in the last decade due to the property of the quantum confinement effects. This makes it possible to increase the band gap by decreasing the size of the crystallites.CQDs made of PbS were in this thesis developed for high PCE (power conversion efficiency), low weight and flexible CQD solar cells. By applying the PbS CQDs on flexible and durable substrates, light weight solar cells with a high power-per-weight of 15.2 W g-1 value were accomplished. Other PbS CQD solar cells were covered and passivated by a mid-high band gap perovskite semiconductor with good lattice matching to facilitate improved stability and PCE, with the latter reaching 10.7 %.

    By shifting the focus towards more low-toxic and more environmentally friendly materials, materials such as PbS could be phased out. In this thesis, Ag2S and AgBiS2 were investigated as alternatives. By utilizing a heat-up synthesis method for the production of Ag2S CQDs, a proof-of-concept PCE of 0.34 % was accomplished for solar cells based on these CQDs. To improve the PCE, a hot-injection method was utilized to produce Ag2S CQDs with a surface better suited for solar cell applications. This resulted in an improved PCE of 2.2 %. In a final study, AgBiS2 CQDs were investigated by changing the composition of the precursor ratio Ag:Bi:S. The final composition was linearly dependent on the Ag precursor concentration. The best device with the highest PCE had a composition close to the stoichiometric ratio of 1:1:2 which was achieved by a precursor composition of 0.72:0.9:1. This resulted in a PCE of 3.3 %.

    The understanding of PbS CQD solar cells and how they can be further improved and ap-plying the relevant information and research to low-toxic alternatives is necessary for the im-provements of these more environmentally friendly CQD materials in solar cell applications.

    Delarbeid
    1. Extremely lightweight and ultra-flexible infrared light-converting quantum dot solar cells with high power-per-weight output using a solution-processed bending durable silver nanowire-based electrode
    Åpne denne publikasjonen i ny fane eller vindu >>Extremely lightweight and ultra-flexible infrared light-converting quantum dot solar cells with high power-per-weight output using a solution-processed bending durable silver nanowire-based electrode
    Vise andre…
    2018 (engelsk)Inngår i: Energy & Environmental Science, ISSN 1754-5692, E-ISSN 1754-5706, Vol. 11, nr 2, s. 354-364Artikkel i tidsskrift (Fagfellevurdert) Published
    Abstract [en]

    Lightweight and flexible solar cells are highly interesting materials for use in new applications, such as spacecraft, aircraft and personal pack load. PbS colloidal quantum dots (CQDs) exhibit a broad and strong light absorption spectrum covering the ultraviolet-visible-near infrared region, allowing for incorporation of very thin CQD films into solar cells with high power conversion efficiency (PCE) from solar light to electricity. Herein, we report an extremely lightweight and ultra-flexible CQD solar cell constructed on a polyethylene naphthalate substrate with a thickness of 1.3 mu m. A solution-processed Ag nanowire network with excellent mechanical, optical and electrical properties was prepared as the front-electrode in the solar cell. The thickness of the complete CQD solar cell is less than 2 mm, and similar to 10% PCE with a weight of 6.5 g m(-2) is achieved, resulting in a power-per-weight output of 15.2 W g(-1). The flexible solar cell possesses durable mechanical properties and maintains high-level photovoltaic performance under extreme deformation and after repeated compression-stretching deformation. Moreover, the flexible CQD solar cell shows impressive stability both under continuous illumination and after storage under ambient conditions. These results reveal that solution-processed CQDs are compatible with an ultra-flexible substrate for the construction of ultra-lightweight infrared light-converting CQD solar cells with possibilities for new exciting solar energy applications.

    sted, utgiver, år, opplag, sider
    ROYAL SOC CHEMISTRY, 2018
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-349844 (URN)10.1039/c7ee02772a (DOI)000425283400013 ()
    Forskningsfinansiär
    Göran Gustafsson Foundation for Research in Natural Sciences and MedicineSwedish Energy AgencySwedish Research Council FormasÅForsk (Ångpanneföreningen's Foundation for Research and Development)Swedish Research CouncilStiftelsen Olle Engkvist Byggmästare
    Tilgjengelig fra: 2018-05-03 Laget: 2018-05-03 Sist oppdatert: 2019-09-21bibliografisk kontrollert
    2. Inorganic CsPbI3 Perovskite Coating on PbS Quantum Dot for Highly Efficient and Stable Infrared Light Converting Solar Cells
    Åpne denne publikasjonen i ny fane eller vindu >>Inorganic CsPbI3 Perovskite Coating on PbS Quantum Dot for Highly Efficient and Stable Infrared Light Converting Solar Cells
    Vise andre…
    2018 (engelsk)Inngår i: Advanced Energy Materials, ISSN 1614-6832, Vol. 8, nr 6, artikkel-id 1702049Artikkel i tidsskrift (Fagfellevurdert) Published
    Abstract [en]

    Solution-processed colloidal quantum dot (CQD) solar cells harvesting the infrared part of the solar spectrum are especially interesting for future use in semitransparent windows or multilayer solar cells. To improve the device power conversion efficiency (PCE) and stability of the solar cells, surface passivation of the quantum dots is vital in the research of CQD solar cells. Herein, inorganic CsPbI3 perovskite (CsPbI3-P) coating on PbS CQDs with a low-temperature, solution-processed approach is reported. The PbS CQD solar cell with CsPbI3-P coating gives a high PCE of 10.5% and exhibits remarkable stability both under long-term constant illumination and storage under ambient conditions. Detailed characterization and analysis reveal improved passivation of the PbS CQDs with the CsPbI3-P coating, and the results suggest that the lattice coherence between CsPbI3-P and PbS results in epitaxial induced growth of the CsPbI3-P coating. The improved passivation significantly diminishes the sub-bandgap trap-state assisted recombination, leading to improved charge collection and therefore higher photovoltaic performance. This work therefore provides important insight to improve the CQD passivation by coating with an inorganic perovskite ligand for photovoltaics or other optoelectronic applications.

    sted, utgiver, år, opplag, sider
    WILEY-V C H VERLAG GMBH, 2018
    Emneord
    charge recombination, inorganic perovskite, quantum dots, solar cells, surface passivation
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-348982 (URN)10.1002/aenm.201702049 (DOI)000426152400017 ()
    Forskningsfinansiär
    Swedish Energy AgencySwedish Research CouncilSwedish Research Council Formas
    Tilgjengelig fra: 2018-04-26 Laget: 2018-04-26 Sist oppdatert: 2019-09-26
    3. Solution-Processed Environmentally Friendly Ag2S Colloidal Quantum Dot Solar Cells with Broad Spectral Absorption
    Åpne denne publikasjonen i ny fane eller vindu >>Solution-Processed Environmentally Friendly Ag2S Colloidal Quantum Dot Solar Cells with Broad Spectral Absorption
    2017 (engelsk)Inngår i: Applied Sciences, E-ISSN 2076-3417, Vol. 7, nr 10, artikkel-id 1020Artikkel i tidsskrift (Fagfellevurdert) Published
    Abstract [en]

    A facile heat-up synthesis route is used to synthesize environmentally friendly Ag2S colloidal quantum dots (CQDs) that are applied as light absorbing material in solid state p-i-n junction solar cell devices. The as-synthesized Ag2S CQDs have an average size of around 3.5 nm and exhibit broad light absorption covering ultraviolet, visible, and near infrared wavelength regions. The solar cell devices are constructed with a device architecture of FTO/TiO2/Ag2S CQDs/hole transport material (HTM) /Au using a solution-processed approach. Different HTMs, N2,N2,N2′,N2′,N7,N7,N7′,N7′-octakis(4-methoxyphenyl)-9,9′-spirobi(9H-fluorene)-2,2′,7,7′ tetramine (spiro-OMeTAD), poly(3-hexylthiophene-2,5-diyl) (P3HT), and poly((2,3-bis(3-octyloxyphenyl)-5,8-quinoxalinediyl)-2,5-thiophenediyl) TQ1 are studied for maximizing the device photovoltaic performance. The solar cell device with P3HT as a hole transport material gives the highest performance and the solar cell exhibit broad spectral absorption. These results indicate that Ag2S CQD have high potential for utilization as environmentally friendly light absorbing materials for solar cell application and that the hole transport material is critical to maximize the solar cell photovoltaic performance.

    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-337578 (URN)10.3390/app7101020 (DOI)000414457800058 ()
    Forskningsfinansiär
    Swedish Energy AgencyGöran Gustafsson Foundation for Research in Natural Sciences and MedicineÅForsk (Ångpanneföreningen's Foundation for Research and Development)Swedish Research Council FormasSwedish Research Council
    Tilgjengelig fra: 2018-01-02 Laget: 2018-01-02 Sist oppdatert: 2019-09-21bibliografisk kontrollert
    4. Hot-Injection Synthesized Ag2S Quantum Dots with Broad Light Absorption and High Stability for Solar Cell Applications
    Åpne denne publikasjonen i ny fane eller vindu >>Hot-Injection Synthesized Ag2S Quantum Dots with Broad Light Absorption and High Stability for Solar Cell Applications
    2018 (engelsk)Inngår i: CHEMNANOMAT, ISSN 2199-692X, Vol. 4, nr 12, s. 1223-1230Artikkel i tidsskrift (Fagfellevurdert) Published
    Abstract [en]

    A hot-injection synthesis method was used to synthesize low-toxicity Ag2S colloidal quantum dots (CQDs) with strong and broad light absorption as an ultra-thin photo-absorber in CQD heterojunction solar cells. By using iodide and sulfur linkers it was possible to accomplish efficient charge carrier extraction, resulting in a high photocurrent due to the broad absorption spectrum. Transient photovoltage decay measurements were used to obtain information about trap states in the CQDs and the effect on the lifetime of the photoinduced carriers. The devices show very promising stability under constant long-term illumination and they are stable under ambient storage conditions with low losses to the performance over a period of over two months. These results show that Ag2S CQDs have high potential within solar cell applications, and point the direction for further improvements.

    Emneord
    energy conversion, hot-injection, quantum dots, silver sulfide, solar cells
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-372703 (URN)10.1002/cnma.201800263 (DOI)000452048600005 ()
    Forskningsfinansiär
    Swedish Energy AgencySwedish Research Council FormasSwedish Research CouncilÅForsk (Ångpanneföreningen's Foundation for Research and Development)
    Tilgjengelig fra: 2019-01-09 Laget: 2019-01-09 Sist oppdatert: 2019-09-21bibliografisk kontrollert
    5. Tuning Size and Composition in Cubic AgBiS2 CQDs for Solar Cells
    Åpne denne publikasjonen i ny fane eller vindu >>Tuning Size and Composition in Cubic AgBiS2 CQDs for Solar Cells
    (engelsk)Manuskript (preprint) (Annet vitenskapelig)
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-393425 (URN)
    Tilgjengelig fra: 2019-09-21 Laget: 2019-09-21 Sist oppdatert: 2019-10-01
  • Molema, Claudia
    et al.
    Tilburg Univ, Dept Tranzo, Sci Ctr Care & Welf, Tilburg, Netherlands;Natl Inst Publ Hlth & Environm, Ctr Nutr Prevent & Hlth Serv, Bilthoven, Netherlands.
    Veldwijk, Jorien
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för folkhälso- och vårdvetenskap, Centrum för forsknings- och bioetik. Natl Inst Publ Hlth & Environm, Ctr Nutr Prevent & Hlth Serv, Bilthoven, Netherlands.
    Wendel-Vos, Wanda
    Natl Inst Publ Hlth & Environm, Ctr Nutr Prevent & Hlth Serv, Bilthoven, Netherlands.
    de Wit, Ardine
    Natl Inst Publ Hlth & Environm, Ctr Nutr Prevent & Hlth Serv, Bilthoven, Netherlands;Univ Med Ctr Utrecht, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands.
    van de Goor, Ien
    Tilburg Univ, Dept Tranzo, Sci Ctr Care & Welf, Tilburg, Netherlands.
    Schuit, Jantine
    Natl Inst Publ Hlth & Environm, Ctr Nutr Prevent & Hlth Serv, Bilthoven, Netherlands;Vrije Univ Amsterdam, Dept Hlth Sci, Amsterdam, Netherlands;EMGO Inst Hlth & Care Res, Amsterdam, Netherlands.
    Chronically ill patients' preferences for a financial incentive in a lifestyle intervention. Results of a discrete choice experiment2019Inngår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 14, nr 7, artikkel-id e0219112Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background The preferences of diabetes type 2 patients and cardiovascular disease patients for a financial incentive added to a specified combined lifestyle intervention were investigated. Methods A discrete choice experiment questionnaire was filled out by 290 diabetes type 2 patients (response rate 29.9%). Panel-mixed-logit models were used to estimate the preferences for a financial incentive. Potential uptake rates of different financial incentives and relative importance scores of the included attributes were estimated. Included attributes and levels were: form of the incentive (cash money and different types of vouchers), value of the incentive (ranging from 15 to 100 euros), moment the incentive is received (start, halfway, after finishing the intervention) and prerequisite for receiving the incentive (registration, attendance or results at group or individual level). Results Prerequisites for receiving the financial incentive were the most important attribute, according to the respondents. Potential uptake rates for different financial incentives ranged between 37.9% and 58.8%. The latter uptake rate was associated with a financial incentive consisting of cash money with a value of (sic)100 that is handed out after completing the lifestyle program with the prerequisite that the participant attended at least 75% of the scheduled meetings. Conclusions The potential uptake of the different financial incentives varied between 37.9% and 58.8%. The value of the incentive does not significantly influence the potential uptake. However, the potential uptake and associated potential effect of the financial incentive is influenced by the type of financial incentive. The preferred type of incentive is (sic)100 in cash money, awarded after completing the lifestyle program if the participant attended at least 75% of the scheduled meetings.

  • Rydberg, Andreas
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Historisk-filosofiska fakulteten, Institutionen för idé- och lärdomshistoria.
    Michael Alberti and the Medical Therapy of the Internal Senses2019Inngår i: Journal of the history of medicine and allied sciences, ISSN 0022-5045, E-ISSN 1468-4373, Vol. 74, nr 3, s. 245-266Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In the first half of the eighteenth century, the German physician Michael Alberti was responsible for hundreds of dissertations and other works in medicine. While the bulk of the production reflected the dominating medical topics of his time, he also developed an original focus on the internal senses and their effects on bodily health and disease. Depending on whether internal senses, such as imagination and memory, were cultivated in the right way or not, they could work as powerful remedies or as equally powerful triggers of disease and even death. This article explores this little known strand of early modern medicine in three steps. First, it shows that Alberti's medicine took form in intimate connection to the Stahlian brand of Pietist medicine. As such, it further elaborated an existing strand of medicine that was intimately connected to German Pietism. Second, it analyses in some detail the role of the internal senses from a pathological and therapeutic perspective as well as examining what kind of persona the physician ought to embody. Lastly, it raises larger questions regarding how to understand this strand of early modern medicine. Rather than approaching it from the perspective of disciplinary history, the article seeks to reconstruct it as a part of what has sometimes been referred to as the early modern cultura animi tradition.

  • Cronert, Axel
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Statsvetenskapliga institutionen. Uppsala Center for Labor Studies.
    Is regulatory compliance by employers possible without enforcement?: Evidence from the Swedish labor market2019Rapport (Annet vitenskapelig)
    Abstract [en]

    This study shines new light on an ongoing debate about the extent to which discouraging enforcement activities are necessary to make regulated actors comply with government regulations. Specifically, it evaluates a long-standing but essentially unenforced regulation that mandated employers in Sweden to post their vacancies at the Public Employment Service (PES) to improve matching and the labor market prospects of disadvantaged workers. Using comprehensive vacancy data from the PES, it tests whether the regulation – despite not being enforced – influenced employers' vacancy posting behavior in the period prior to its partial repeal in 2007. Exploiting the fact that the repeal did not apply to employers in the central government sector, the difference-in-differences analyses conducted in this study identify a substantial and significant negative effect of repealing the unenforced law on employers' vacancy posting behavior, under reasonable assumptions. This finding is at odds with standard deterrence models of regulatory compliance and hints at an important role for organizational factors related to cultures and norms. A supplementary analysis of heterogeneous effects among local government employers investigates to what extent some organizational factors are correlated to compliance with the unenforced regulation.

  • Cronert, Axel
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Statsvetenskapliga institutionen. Uppsala Center for Labor Studies.
    Kan regelefterlevnad uppnås utan hot om påföljder?: En utvärdering av lagen om allmän platsanmälan2019Rapport (Annet (populærvitenskap, debatt, mm))
    Abstract [sv]

    Studien som sammanfattas i denna rapport syftar till att undersöka i vilken mån lagar och regler kan påverka beteendet hos berörda aktörer utan att kombineras med tillsyn och hot om påföljder. Studien utvärderar lagen (1976:157) om allmän platsanmälan, som under trettio års tid föreskrev arbetsgivare i Sverige en skyldighet att i samband med rekrytering anmäla sina lediga platser till Arbetsförmedlingen. När lagen upphävdes i juli 2007 hade dess efterlevnad under en mycket lång tid inte alls kontrollerats. För att undersöka i vilken mån arbetsgivare trots detta efterlevde regelverket utnyttjar studien det faktum att lagens upphävande inte kom att beröra statliga arbetsgivare och därmed kan utvärderas genom jämförelser mellan sektorer. Utvärderingen visar att upphävandet av lagen ledde till en signifikant minskning av privata och kommunala arbetsgivares benägenhet att anmäla lediga platser. Det innebär således att lagen hade en beteendeeffekt trots avsaknaden av tillsyn och hot om påföljder. I en kompletterande analys av kommunala arbetsgivare undersöks i vilken mån ett antal organisatoriska faktorer samvarierar med benägenheten att efterleva regelverket trots avsaknaden av kontroll.

  • Port, Elof
    et al.
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Företagsekonomiska institutionen.
    Hammar, Erik
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Företagsekonomiska institutionen.
    Har insynshandel blivit en frihandel?2019Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Denna studie undersöker insynspersoners genomsnittliga kumulativa abnormala avkastning vid köp av aktier 31–40 kalenderdagar innan offentliggörandet av kvartalsrapporter på Stockholmsbörsen. Genom att använda en standardiserad eventstudie som metod, finner vi att insynspersoner erhåller en genomsnittlig kumulativ abnormal avkastning vid köp av aktier de första 30 handelsdagarna. Vi finner även att handelsdag 31–60 vidareutvecklas den positiva kumulativa abnormala avkastningen i samma riktning vilket tyder på att post-earningsannouncement drift förekommer på Stockholmsbörsen. Vi finner dessutom att när insynsköp sker inför kvartalsrapporter erhåller 47% av alla insynsköp en positiv kumulativ abnormal avkastning handelsdag 0–30 på 1% signifikansnivå. Resultaten ger en indikation på att insynshandel förekommer på den svenska aktiemarknaden och att insynsköp inför kvartalsrapporter är en tidig signal om att post-earnings-announcement drift kan inträffa efter kommande rapport.

  • Aaboud, M.
    et al.
    Asimakopoulou, Eleni M.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Högenergifysik.
    Bergeås Kuutmann, Elin
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Högenergifysik.
    Bokan, Petar
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Högenergifysik.
    Brenner, Richard
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Högenergifysik.
    Ekelöf, Tord
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Högenergifysik.
    Ellajosyula, Venugopal
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Högenergifysik.
    Ellert, Mattias
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Högenergifysik.
    Ferrari, Arnaud
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Högenergifysik.
    Gradin, P. O. Joakim
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Högenergifysik.
    Isacson, Max
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Högenergifysik.
    Mårtensson, Mikael
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Högenergifysik.
    Sales De Bruin, Pedro
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Högenergifysik.
    Zwalinski, L.
    Dijet azimuthal correlations and conditional yields in pp and p+Pb collisions at √sNN=5.02TeV with the ATLAS detector2019Inngår i: Physical Review C: Covering Nuclear Physics, ISSN 2469-9985, E-ISSN 2469-9993, Vol. 100, nr 3, artikkel-id 034903Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    This paper presents a measurement of forward-forward and forward-central dijet azimuthal angular correlations and conditional yields in proton-proton (pp) and proton-lead (p + Pb) collisions as a probe of the nuclear gluon density in regions where the fraction of the average momentum per nucleon carried by the parton entering the hard scattering is low. In these regions, gluon saturation can modify the rapidly increasing parton distribution function of the gluon. The analysis utilizes 25 pb(-1) of pp data and 360 mu b(-1) of p + Pb data, both at root S-NN = 5.02 TeV, collected in 2015 and 2016, respectively, with the ATLAS detector at the Large Hadron Collider. The measurement is performed in the center-of-mass frame of the nucleon-nucleon system in the rapidity range between -4.0 and 4.0 using the two highest transverse-momentum jets in each event, with the highest transverse-momentum jet restricted to the forward rapidity range. No significant broadening of azimuthal angular correlations is observed for forward-forward or forward-central dijets in p + Pb compared to pp collisions. For forward-forward jet pairs in the proton-going direction, the ratio of conditional yields in p + Pb collisions to those in pp collisions is suppressed by approximately 20%, with no significant dependence on the transverse momentum of the dijet system. No modification of conditional yields is observed for forward-central dijets.

  • san Martin Bucht, Michelle
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Geovetenskapliga sektionen, Institutionen för geovetenskaper.
    Overcoming Scale Challenges in Policies Through Analysing Governance Architecture: The Case of Chemical Management Policies in Sweden2019Independent thesis Advanced level (degree of Master (Two Years)), 20 poäng / 30 hpOppgave
    Abstract [en]

    The release of harmful chemicals into the Earth system is a threat to the survival of human societies and ecosystems. There are different types of harmful chemicals, some possess characteristics that are more harmful than others. Chemicals causing local effects have a short-term impact on the Earth system, whereas others are persistent in nature. The second becomes a global pollution issue because these substances accumulate, resulting in cascades through the Earth system. The chemical pollution issue is a scale challenge, meaning that the pollution issue occurs within and across levels and scales. Chemical pollution is not treated as a scale challenge in the decision-making process today. In the literature it is stated that chemical pollution is usually studied by focusing on single institutions, which hinders the possibility to investigate the linkages between the institutions affecting the scale dynamics. In this study the scale and cross-scale perspective was used to identify institutions that are affecting the work for a toxin free environment on a national and municipality level in Sweden. Governance Architecture was used as an analytical tool to investigate the key hindering and facilitating governance mechanisms to achieve sustainable chemical management in Sweden from a cross-scale perspective. The analytical tool was based on the Earth System Governance theory and scale dynamics. Sweden was used as a case to apply this tool. Two methods were used to gather empirics: literature analysis and in-depth interviews. The findings reveal an overrepresentation of the hindering factors being a result of a scale or a policy gap issue. These gaps are created by the lack of interdisciplinarity in the decision-making process, and the current legislation and politics which influences the implementation of the strategies on the different institutional levels. To overcome these hinders the current strategies and legislation needs to be in collaboration. This study contributes to empiric development within policy studies on how the chemical pollution issue needs to be conceptually formulated to achieve a toxin free environment within and cross-scales. It also contributes to method development by addressing the gap of including several institutions in cross-scale studies.

  • Rodríguez Medina, Samuel
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Språkvetenskapliga fakulteten, Institutionen för lingvistik och filologi.
    Multi-Label Text Classification with Transfer Learning for Policy Documents: The Case of the Sustainable Development Goals2019Independent thesis Advanced level (degree of Master (Two Years)), 20 poäng / 30 hpOppgave
    Abstract [en]

    We created and analyzed a text classification dataset from freely-available web documents from the United Nation's Sustainable Development Goals. We then used it to train and compare different multi-label text classifiers with the aim of exploring the alternatives for methods that facilitate the search of information of this type of documents.

    We explored the effectiveness of deep learning and transfer learning in text classification by fine-tuning different pre-trained language representations — Word2Vec, GloVe, ELMo, ULMFiT and BERT. We also compared these approaches against a baseline of more traditional algorithms without using transfer learning. More specifically, we used multinomial Naive Bayes, logistic regression, k-nearest neighbors and Support Vector Machines.

    We then analyzed the results of our experiments quantitatively and qualitatively. The best results in terms of micro-averaged F1 scores and AUROC are obtained by BERT. However, it is also interesting that the second best classifier in terms of micro-averaged F1 scores is the Support Vector Machines, closely followed by the logistic regression classifier, which both have the advantage of being less computationally expensive than BERT. The results also show a close relation between our dataset size and the effectiveness of the classifiers.

  • Larsson, Jonna
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Kulturgeografiska institutionen.
    Afrika i läroboken: En kritisk diskursanalys av läroböcker i geografi2019Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Syftet med den här uppsatsen är att undersöka hur Afrika och afrikaner presenteras och skildras i läroböcker i geografi från 70-talet och framåt. Studien ämnar även ge en omfattande beskrivning av diskursens förändring. Genom en kritisk diskursanalys med utgångspunkt i postkolonial teori ämnar studien blottlägga etnocentriska, stereotypa och andrafierande uttryck och föreställningar i geografiska läromedel. I studien analyseras tio läroböcker från olika förlag och författare från 70-talet och fram till de böcker som används i nutida undervisning. Studien lyfter fram den allmänna bild av Afrika som läroböckerna förmedlar genom beskrivningar och skildringar av den afrikanska kontinenten, och huruvida den bilden understryker koloniala idéer och strukturer. Analysen visar på en tydlig förändring i läroböckerna övertid i avseende av förekomsten av stereotyper, etnocentrism och fördomsfulla uttryck. Vidare har beskrivningarna av Afrika och afrikaner i läroböckerna blivit mer nyanserade med en bredare representation av den afrikanska befolkningen. Fortfarande tenderar även de nyare läroböckerna att förmedla en utvecklingspessimistisk bild av Afrika, där västvärlden framstår som aktör för utveckling och välstånd.

  • Österlund, Dennis
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Kulturgeografiska institutionen.
    Miljöproblematisk representation: En analys av geografiska läromedel2019Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Denna analys ämnade att undersöka hur ämnesstoff gällande miljöproblem i geografiska läromedel representeras. Representationen grundades på teorier om selektiva traditioner, utbildningsfilosofier och didaktiska typologier. Analysen har också undersökt hur detta innehåll har förändrats över tid i de utvalda läromedlen.

          De utvalda läromedlen publicerades 1982, 1996 och 2013 och analyserades utifrån en kvalitativ textanalys. Baserat på tidigare forskning och teori kan denna undersökning konstatera att innehållsstoffet i geografiböcker som rör miljöproblem representeras ur olika perspektiv. Det analyserade stoffet har representerats ur ett naturvetenskapligt perspektiv, ett samhällsvetenskapligt perspektiv eller ett tvärvetenskapligt perspektiv. Detta beroende på hur författaren eller författarna vill förmedla fakta och kunskap till eleverna. Vad som påverkar det perspektiv som texten skrivs ur kan vara medvetet eller omedvetet baserat på den rådande samhällsdebatten, moraliska och politiska frågor, den aktuella pedagogiska synen och utbildningstraditioner. Analysen har även kunnat konstatera att ämnesstoffet gällande miljöproblem har ändrats över tid. Teman som till exempel olja och fossila bränslen har representerats på olika sätt, tagit olika stor plats i läromedlen samt behandlats ur olika perspektiv med olika utgångspunkter.

  • Bogren, Ella
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Statsvetenskapliga institutionen.
    “The pain she feels, I don’t feel it, but I feel for her”: A case study of urban teenage schoolboys’ knowledge and attitudes towards menstruation in Ghana2019Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [en]

    Menstrual health management can be a difficulty for menstruating women and girls, especially in low- and middle-income countries or other areas of poverty. Menstruation being characterized by stigmatisation, myths and taboo makes it especially troublesome, preventing women and girls to handle their menstruation safely and with dignity. Male attitudes have been argued to play an important role in perpetuating these stigmas and taboos, yet little is known about them. This study sets out to investigate male menstrual knowledge and attitudes, the role of religion in shaping menstrual attitudes and the potential consequences for menstruating women and girls. Qualitative data from group interviews with 24 boys aged 15-19 in a Senior High School in Accra, Ghana is used as basis for analysis. The results are organised along three themes, reflecting the three sub-research questions guiding the study. Findings demonstrate how schoolboys have an elemental understanding of the physiological process of menstruation yet demonstrate a deep understanding of cultural restrictions and the way menstruation may be experienced. Attitudes contain both positive and negative elements, including menstruation as normal and natural on the one hand, and the menstruating girl as unclean and impure on the other. Religion seem to play in important role in perpetuating negative menstrual attitudes, reinforcing the idea of menstruation as impure and unclean. Potential consequences of these attitudes risk menstruation continuing being considered as unclean and impure in addition to be neglected as a “girl’s matter”. However, respondents also identified menstrual difficulties which may foster supportive involvement in menstruation. The findings suggest the importance of continuing to address the surrounding communities of menstruating women and girls, including within and outside of educational and religious institutions.

  • Lundh, Jenni
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Statsvetenskapliga institutionen.
    Den svenska klimatdiskursen: En diskursanalys av folkets klimatåsikter i nyhetsmedia2019Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • Disputas: 2019-11-05 09:15 Häggsalen, Uppsala
    Redzwan Mohd Shah, Syaiful
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets elektronik.
    Prospective Applications of Microwaves in Medicine: Microwave Sensors for Orthopedic Monitoring and Burn Depth Assessment2019Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    In recent years, the use of microwave techniques for medical diagnostics has experienced impressive developments. It has demonstrated excellent competencies in various modalities such as using non-ionizing electromagnetic waves, providing non-invasive diagnoses, and having the ability to penetrate human tissues within the GHz range. However, due to anatomical, physiological, and biological variations in the human body, certain obstacles are present. Moreover, there are accuracy problems such as the absence of numerical models and experimental data, difficulty in conducting tests due to safety issues with human subjects, and also practical restrictions in clinical implementation. With the presence of these issues, a better understanding of the microwave technique is essential to further improve its medical application and to introduce alternative diagnostic methods that can detect and monitor various medical conditions in real time.

    The first part of this thesis focuses on measurement systems for the microwave technique in terms of sensor design and development, numerical analysis, permittivity measurement, and phantom fabrication. The aim is to investigate the feasibility of flexible systems with different fields of application including a microwave sensor system for measuring the healing progression of bone defects present in lower extremity trauma, bone regeneration in craniotomy for craniosynostosis treatments, and dielectric variation for burn injuries. The microwave sensor which utilizes the contrast in dielectric constant between various tissues was used as the primary sensor for the proposed application. This involved detailed optimization of the sensor for greater sensitivity. The experimental work carried out in the lab environment showed that the microwave sensor was able to detect the contrast in dielectric properties so that it can give an indication of the healing status for actual clinical scenarios.

    The second part of the thesis is making a significant step towards its practical implementation by establishing a system that can detect and monitor the rate of healing progression with fast data acquisition speed of microseconds, and developing an efficient user interface to convert raw microwave data into legible clinical information in terms of bone healing and burn injuries. As an extension to this thesis, clinical studies were conducted and ethical approval for conducting tests on human subjects was obtained for the development of a microwave medical system. The results showed a clear difference in healing progressions due to high detection capability in terms of dielectric properties of different human tissues. All of these contributions enable a portable system to complement existing medical applications with the aim of providing more advanced healthcare systems.

    Delarbeid
    1. Split-Ring Resonator Sensor Penetration Depth Assessment Using In Vivo Microwave Reflectivity and Ultrasound Measurements for Lower Extremity Trauma Rehabilitation
    Åpne denne publikasjonen i ny fane eller vindu >>Split-Ring Resonator Sensor Penetration Depth Assessment Using In Vivo Microwave Reflectivity and Ultrasound Measurements for Lower Extremity Trauma Rehabilitation
    Vise andre…
    2018 (engelsk)Inngår i: Sensors, ISSN 1424-8220, E-ISSN 1424-8220, Vol. 18, nr 2, artikkel-id 636Artikkel i tidsskrift (Fagfellevurdert) Published
    Abstract [en]

    In recent research, microwave sensors have been used to follow up the recovery of lower extremity trauma patients. This is done mainly by monitoring the changes of dielectric properties of lower limb tissues such as skin, fat, muscle, and bone. As part of the characterization of the microwave sensor, it is crucial to assess the signal penetration in in vivo tissues. This work presents a new approach for investigating the penetration depth of planar microwave sensors based on the Split-Ring Resonator in the in vivo context of the femoral area. This approach is based on the optimization of a 3D simulation model using the platform of CST Microwave Studio and consisting of a sensor of the considered type and a multilayered material representing the femoral area. The geometry of the layered material is built based on information from ultrasound images and includes mainly the thicknesses of skin, fat, and muscle tissues. The optimization target is the measured S-11 parameters at the sensor connector and the fitting parameters are the permittivity of each layer of the material. Four positions in the femoral area (two at distal and two at thigh) in four volunteers are considered for the in vivo study. The penetration depths are finally calculated with the help of the electric field distribution in simulations of the optimized model for each one of the 16 considered positions. The numerical results show that positions at the thigh contribute the highest penetration values of up to 17.5 mm. This finding has a high significance in planning in vitro penetration depth measurements and other tests that are going to be performed in the future.

    sted, utgiver, år, opplag, sider
    MDPI, 2018
    Emneord
    Microwave measurement, ultrasound measurement, split-ring resonator, penetration depth, human lower extremity, sensor, model optimization, multilayered material, electric field distribution
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-352995 (URN)10.3390/s18020636 (DOI)000427544000319 ()29466312 (PubMedID)
    Forskningsfinansiär
    Swedish Research Council, 2017-04644
    Tilgjengelig fra: 2018-07-16 Laget: 2018-07-16 Sist oppdatert: 2019-09-17bibliografisk kontrollert
    2. COMplex Fracture Orthopedic Rehabilitation (COMFORT) - Real-time visual biofeedback on weight bearing versus standard training methods in the treatment of proximal femur fractures in the elderly: study protocol for a multicenter randomized controlled trial
    Åpne denne publikasjonen i ny fane eller vindu >>COMplex Fracture Orthopedic Rehabilitation (COMFORT) - Real-time visual biofeedback on weight bearing versus standard training methods in the treatment of proximal femur fractures in the elderly: study protocol for a multicenter randomized controlled trial
    2018 (engelsk)Inngår i: Trials, ISSN 1745-6215, E-ISSN 1745-6215, Vol. 19, artikkel-id 220Artikkel i tidsskrift (Fagfellevurdert) Published
    Abstract [en]

    Background:

    Proximal femur fractures are a common injury after low energy trauma in the elderly. Most rehabilitation programs are based on restoring mobility and early resumption of weight-bearing. However, therapy compliance is low in patients following lower extremity fractures. Moreover, little is known about the relevance of gait parameters and how to steer the rehabilitation after proximal femur fractures in the elderly. Therefore, the aim of this prospective, randomized controlled trial is to gain insight in gait parameters and evaluate if real-time visual biofeedback can improve therapy compliance after proximal femur fractures in the elderly.

    Methods:

    This is a two-arm, parallel-design, prospective, randomized controlled trial. Inclusion criteria are age >= 60 years, a proximal femur fracture following low energy trauma, and unrestricted-weight bearing. Exclusion criteria are cognitive impairment and limited mobility before trauma. Participants are randomized into either the control group, which receives care as usual, or the intervention group, which receives real-time visual biofeedback about weight-bearing during gait in addition to care as usual. Spatiotemporal gait parameters will be measured in 94 participants per group during a 30-m walk with an ambulatory biofeedback system (SensiStep). The progress of rehabilitation will be evaluated by the primary outcome parameters maximum peak load and step duration in relation to the discharge date. Secondary outcome parameters include other spatiotemporal gait parameters in relation to discharge date. Furthermore, the gait parameters will be related to three validated clinical tests: Elderly Mobility Scale; Functional Ambulation Categories; and Visual Analogue Scale. The primary hypothesis is that participants in the intervention group will show improved and faster rehabilitation compared to the control group.

    Discussion:

    The first aim of this multicenter trial is to investigate the normal gait patterns after proximal femur fractures in the elderly. The use of biofeedback systems during rehabilitation after proximal femur fractures in the elderly is promising; therefore, the second aim is to investigate the effect of real-time visual biofeedback on gait after proximal femur fractures in the elderly. This could lead to improved outcome. In addition, analysis of the population may indicate characteristics of subgroups that benefit from feedback, making a differentiated approach in rehabilitation strategy possible.

    Emneord
    Proximal femur fracture, Weight-bearing, Biofeedback, Gait analysis, SensiStep, Fracture rehabilitation
    HSV kategori
    Identifikatorer
    urn:nbn:se:uu:diva-353203 (URN)10.1186/s13063-018-2612-9 (DOI)000429992800001 ()29650034 (PubMedID)
    Tilgjengelig fra: 2018-06-13 Laget: 2018-06-13 Sist oppdatert: 2019-09-17
    3. Improved Sensor for Non-invasive Assessment of Burn Injury Depth Using Microwave Reflectometry
    Åpne denne publikasjonen i ny fane eller vindu >>Improved Sensor for Non-invasive Assessment of Burn Injury Depth Using Microwave Reflectometry
    Vise andre…
    2019 (engelsk)Inngår i: 2019 13th European Conference on Antennas and Propagation (EuCAP), 2019Konferansepaper, Publicerat paper (Fagfellevurdert)
    Abstract [en]

    The European project “Senseburn” aims to develop a non-invasive diagnostic instrument for assessing the depth and propagation of human burns in the clinical scenario. This article introduces an improved flexible microwave split-ring resonator-based sensor, as a new development in this project. The excitation system and the fabrication process are the major improvements with respect to its precedent microwave sensor, both based in polydimethylsiloxane (PDMS) and copper. Both improvements are introduced together with the design of the sensor and of the experimental setup. Human tissue emulating phantoms are designed, fabricated, validated, and employed to emulate different burn depths and to validate the conceptual functionality of the proposed sensor. The Keysight dielectric probe 85070E is employed for the phantom validation. The analysis suggests that the sensor could estimate the burn depth. Future works will be carried out with ex vivo human tissues. 

    HSV kategori
    Forskningsprogram
    Teknisk fysik med inriktning mot mikrovågsteknik
    Identifikatorer
    urn:nbn:se:uu:diva-390800 (URN)000480384702154 ()978-88-907018-8-7 (ISBN)
    Konferanse
    2019 13th European Conference on Antennas and Propagation (EuCAP), 31 March-5 April 2019, Krakow, Poland
    Tilgjengelig fra: 2019-08-14 Laget: 2019-08-14 Sist oppdatert: 2019-09-25bibliografisk kontrollert
    4. Microwave-Sensor-Based Clinical Measurements for Monitoring Post-Craniotomy Bone Development in Pediatric Craniosynostosis Patients
    Åpne denne publikasjonen i ny fane eller vindu >>Microwave-Sensor-Based Clinical Measurements for Monitoring Post-Craniotomy Bone Development in Pediatric Craniosynostosis Patients
    Vise andre…
    (engelsk)Inngår i: Artikkel i tidsskrift (Fagfellevurdert) Submitted
    Abstract [en]

    The bone density analysis system is a new method to analyze the amount of bone healed in cranial vault defects. Craniotomy creates such defects to treat pediatric patients with craniosynostosis. This method uses the variations in the resonance of a microwave sensor attached to the area under test in the patient. Data were collected from infants treated for craniosynostosis through craniotomy surgery in clinical trials. The data, which consist of resonance parameters (frequency, amplitude, and Q-factor) is collected using a microwave-based biomedical sensor. This sensor could detect changes in the resonance as changes in the permittivity of the various tissues at the ISM band. By observing differences between a reference and target defect points, bone healing over time could be accessed. In this paper, we analyze the validity of a proposed Computational Simulation Technology (CST) based numerical model for the sensor and extend the clinical data analysis from previous works with our bone density analysis system. The validity of the model is analyzed by comparing its outcomes to available measurements from numerical simulations, phantoms mimicking living tissues and clinical trial. In the data analysis, a hypothesis is formulated and tested regarding the healing over time. By deriving a set of parameters for each collected dataset in the clinical trials, a distinct pattern was found which shows visible changes such as edema and tissue thickening over the course of the healing process with this technique. Moreover, we manage to distinguish significant differences between the reference and defect points after the craniosynostosis surgery.

    HSV kategori
    Forskningsprogram
    Teknisk fysik med inriktning mot mikrovågsteknik
    Identifikatorer
    urn:nbn:se:uu:diva-392809 (URN)
    Tilgjengelig fra: 2019-09-10 Laget: 2019-09-10 Sist oppdatert: 2019-09-23
    5. Analysis of Thickness Variation in Biological Tissues using Microwave Sensors for Health Monitoring Applications
    Åpne denne publikasjonen i ny fane eller vindu >>Analysis of Thickness Variation in Biological Tissues using Microwave Sensors for Health Monitoring Applications
    Vise andre…
    (engelsk)Inngår i: Artikkel i tidsskrift (Fagfellevurdert) Submitted
    Abstract [en]

    Microwave sensing technique is a possible and attractive alternative modality to standard Xrays,magnetic resonance imaging, and computed tomography methods for medical diagnostic applications.This technique is beneficial since it uses non-ionizing radiation and that can be potentially used for themicrowave healthcare system. The main purpose of this paper is to present a microwave sensing techniqueto analyze the variations in biological tissue thickness, considering the effect of physiological and biologicalproperties on microwave signals. With this goal, we have developed a two-port non-invasive sensor systemcomposed of two split ring resonators (SRRs) operating at an Industrial, Scientific, and Medical frequencyband of 2.45 GHz. The system is verified using the amplitude and phase of the transmitted signal in ex-vivomodels, representing different tissue thicknesses. Clinical applications such as the diagnosis of muscularatrophy can be benefitted from this study.

    HSV kategori
    Forskningsprogram
    Teknisk fysik med inriktning mot mikrovågsteknik
    Identifikatorer
    urn:nbn:se:uu:diva-392816 (URN)
    Tilgjengelig fra: 2019-09-10 Laget: 2019-09-10 Sist oppdatert: 2019-09-23
  • Iggström, Sofia
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för ekologi och genetik.
    Investigation of the role of insulin receptor genes in wing polyphenism using gene knockdown and differential gene expression analysis in the non-model organism Gerris buenoi2019Independent thesis Advanced level (professional degree), 20 poäng / 30 hpOppgave
    Abstract [en]

    Wing polyphenism is a type of phenotypic plasticity present in several insect species whereby a genotype have the ability to develop alternative wing morphs when exposed to different environmental cues. One organism demonstrating a clear case of wing polyphenism is the water strider species, Gerris buenoi, which develop long- or short wings depending on exposure to different photoperiods (the time the organism is exposed to light during a 24 h period). The molecular mechanism behind wing polyphenism in insects in general, and in water striders in particular, is largely unknown. From a study on wing polyphenism in the Brown planthopper (Nilaparvata lugens), some candidate genes have been identified and include two insulin receptor genes and the Forkhead transcription factor (FOXO). Since these genes have been demonstrated to affect wing polyphenism in Brown planthopper (BPH) and since G. buenoi contains an additional insulin receptor homolog, the potential role of these genes in regulating wing polyphenism in G. buenoi have in this project been investigated. The functional genetic technique RNA interference (RNAi) was used to evaluate the function of the genes. This method knock down gene expression in the genes mentioned above, one at a time, to investigate if they have a function in wing polyphenism in G. buenoi. DsRNA with specific homology to each target gene was successfully produced. However, when attempting to inject the dsRNA through micro injection all injected liquid leaked out from the body cavity, and the RNAi was therefore not successful. Further optimisation of the injection protocol has to be done to be able to perform RNAi properly in the future. Thereafter, RT-qPCR was used to evaluate whether the insulin receptor genes and FOXO are differentially expressed between the two photoperiods giving rise to the different wing morphs. The differential gene expression experiment showed differences between the mRNA levels of all target genes between G. buenoi being reared in the two different photoperiods. More specific upregulation of the genes FOXO and insulin receptor 2 in short winged G. buenoi were demonstrated. Further, insulin receptor 1-like, was also demonstrated to be upregulated in the short winged morph. Results presented in this project are in line with the previously identified regulation pattern in BPH, still the results need further evaluation. Since gene expression differences were present for all candidate genes between G. buenoi reared in the different photoperiods, theses genes could still be seen as potential candidate genes in wing polyphenism in water striders.

  • Ahlgren, Thea
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Statsvetenskapliga institutionen.
    Att bryta ner och avhumanisera en människa - En kvalitativ studie om kvinnliga riksdagsledamöters erfarenheter av och strategier för könade kränkningar på sociala medier2019Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave