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  • Boeije, M. F. J.
    et al.
    Delft Univ Technol, Fundamental Aspects Mat & Energy, Fac Sci Appl, Mekelweg 15, NL-2629 JB Delft, Netherlands..
    Delczeg-Czirjak, Erna Krisztina
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, Materials Theory.
    van Dijk, N. H.
    Delft Univ Technol, Fundamental Aspects Mat & Energy, Fac Sci Appl, Mekelweg 15, NL-2629 JB Delft, Netherlands..
    Eriksson, Olle
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, Materials Theory.
    Bruck, E.
    Delft Univ Technol, Fundamental Aspects Mat & Energy, Fac Sci Appl, Mekelweg 15, NL-2629 JB Delft, Netherlands..
    On the phase stability of CaCu5-type compounds2017In: Journal of Alloys and Compounds, ISSN 0925-8388, E-ISSN 1873-4669, Vol. 722, 549-554 p.Article in journal (Refereed)
    Abstract [en]

    We present a hybrid method to inspect the phase stability of compounds having a CaCu5-type crystal structure. This is done using 2D stability plots using the Miedema parameters that are based on the work function and electron density of the constituent elements. Stable compounds are separated from unstable binary compounds, with a probability of 94%. For stable compounds, a linear relation is found, showing a constant ratio of charge transfer and electron density mismatch. DFT calculations show the same trend. Elements from the s, d, f-block are all reliably represented, elements from the p-block are still challenging.

  • Wikborg, Jonas
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Arts, Department of Archaeology and Ancient History.
    Skelettgravarna på Bastubacken: Skelettgravskicket i Mälardalen under romersk järnålder.1996In: TOR, ISSN 0495-8772, Vol. 28, 105-152 p.Article in journal (Other academic)
  • Erixson, Oscar
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Social Sciences, Department of Economics, Uppsala Center for Fiscal Studies. Res Inst Ind Econ IFN, S-10401 Stockholm, Sweden..
    Health responses to a wealth shock: evidence from a Swedish tax reform2017In: Journal of Population Economics, ISSN 0933-1433, E-ISSN 1432-1475, Vol. 30, no 4, 1281-1336 p.Article in journal (Refereed)
    Abstract [en]

    This paper makes two contributions to the literature on the effects of wealth on health. First, it deals with reverse causality and omitted variable bias by exploiting exogenous variation in inherited wealth generated by the repeal of the Swedish inheritance tax. Second, it analyzes responses in health outcomes through the use of administrative registers. The results show that increased wealth has limited short to medium run impacts on objective adult health. This is in line with what has previously been reported in the literature.

  • Isendahl, Christian
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Arts, Department of Archaeology and Ancient History, African and Comparative Archaeology.
    The Archaeology of the Lowland Maya and in Eastern Africa: A Discussion of Some Selected Common Issues.1996In: TOR, ISSN 0495-8772, Vol. 28, 91-104 p.Article in journal (Other academic)
  • Dahl, Caroline
    Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences.
    Källspårning och åtgärdsförslag för rening av dagvatten från Lunda industriområde i Stockholm2017Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    When more and more people move to the city and the cities expand, so does the amount of impermeable surfaces. This leads to reduced surfaces where rain is allowed to infiltrate the ground. This in turn leads to an increased amount of surface runoff and large amounts of storm water within our cities. On its way through the city the storm water adsorbs pollutants that have been deposited on the surface by traffic and from the air and transports them to nearby waterways and lakes. Since storm water is often released to the recipient without treatment it can have a significant effect on the water quality.

    Bällstaån is one waterway that receives large amounts of storm water from urbanized areas which has resulted in it being one of the most polluted waterways in Stockholm. Mainly high concentrations of nutrients, such as phosphorus, nitrogen, and metals have been recorded during the most recent sampling in 2016. High concentrations of PAH and PFOS have also been detected and to improve the chemical and ecological status of the waterway possible sources of these contaminants are being investigated within the area.

    One possible source that has been identified is storm water from Lunda industrial area located within the runoff area of Bällstaån. Lunda industrial area is about 76 ha and is mainly composed of a mix of industry buildings and offices. To determine potential sources of contaminants within the area an inventory of the site was conducted as well as a storm water simulation in the model SEWSYS with site specific data within this project. The results show that traffic is the largest contributor to pollutants in the storm water from the area but also that zinc corrosion, mainly from fences, is the largest source of zinc. The results from SEWSYS also show that storm water from roofs had lower concentrations of metals and PAH than storm water from roads and other hard surfaces on the ground.

    To improve the quality of the storm water from the area and reduce concentrations of pollutants several different methods were investigated. Since the area is an urban area with limited amount of open areas for large-scale treatment of storm water mainly small-scale methods have been investigated. Since the soil matrix is mainly composed of hard rock and clay the infiltration capacity of the area is considered poor and therefore solutions only based on infiltration have been deemed unsuitable. The main focus of the project has been on investigating how well the methods can treat the water but their ability to delay and store water have also been briefly estimated.

    The solutions considered to be most suitable for the area are ditches or trenches in connection to roads since most of the contaminants originate from these areas and these methods have a high reduction capacity for most contaminants. Biofilters or permeable surfaces could also be used as a complement to increase the amount of storm water that can be treated and stored and to reduce the amount of impermeable surfaces within the area and thereby reduce the amount of storm water in the first place. For roof runoff both biofilters and underground storage performed well enough to reduce concentrations of all pollutants except nitrogen below the guidelines.

  • Kristensson, Anna
    et al.
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Arts, Department of Archaeology and Ancient History.
    Olsson, Carina
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Arts, Department of Archaeology and Ancient History.
    Ecofacts Indicating Late Neolithic and Early Bronze Age Farming in Sweden1996In: Tor: meddelanden från Uppsala universitets museum för nordiska fornsaker, ISSN 0495-8772, Vol. 28, 53-68 p.Article in journal (Other academic)
  • Lindgren, Christina
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Arts, Department of Archaeology and Ancient History.
    Kvarts som källmaterial: exempel från den mesolitiska boplatsen Hagtorp1996In: TOR, ISSN 0495-8772, Vol. 28, 29-52 p.Article in journal (Other academic)
  • Topsholm, Max
    CHARM Transformation: A case study on change and release management Catella Bank2017Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    The purpose of the thesis is to conduct a case study for investigating change and release management at Catella Bank, within the context of IT Service Management (ITSM), by measuring and to provide suggestions for improvements. Incident and operations management is included to enhance the understanding of the historical performance at Catella. The case study is set out to answer the following research questions:

    1. “How does a transition in change management structure impact the performance of successfully delivering both changes and releases of IT services at a financial institution?”

    2. “What are the causes of delays in the delivering changes and releases?”

    3. “How does stakeholder involvement alter the performance of implementing a successful change and release?”

    4. “How do Information Technology, and the corresponding departments manage and control necessary changes and releases of software at present?”

    Research methodology utilized in the thesis includes both qualitative and quantitative research, including interviews, participation in meetings and empirical investigation of internal material at Catella.

    The result from the research has provided a significant collection of issues, as well as suggested solutions for Catella to take to improve organizational maturity in enhancing the capabilities in performing work related to the four managerial disciplines within ITSM. The research culminated in the creation of the CHARM (CHange And Release Management) model, which consists of integrating change and release management into project management, split between three different components for the three organizational levels. The author has created the following components: Strategy matrix, a governance model, and a process model.

  • Boström, Adrian
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
    Ciuculete, Diana-Maria
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
    Attwood, Misty M.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
    Krattinger, Regina
    Univ Zurich, Univ Zurich Hosp, Dept Clin Pharmacol & Toxicol, Zurich, Switzerland..
    Nikontovic, Lamia
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
    Titova, Olga E
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
    Kullak-Ublick, Gerd A.
    Univ Zurich, Univ Zurich Hosp, Dept Clin Pharmacol & Toxicol, Zurich, Switzerland..
    Mwinyi, Jessica
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
    Schiöth, Helgi B.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Pharmacology. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
    A MIR4646 associated methylation locus is hypomethylated in adolescent depression2017In: Journal of Affective Disorders, ISSN 0165-0327, E-ISSN 1573-2517, Vol. 220, 117-128 p.Article in journal (Refereed)
    Abstract [en]

    Background: Studies of epigenetics and transcriptional activity in adolescents may provide knowledge about possible preventive strategies of depression. Methods: We present a methylome-wide association study (MWAS) and cohort validation analysis of depression in adolescents, in two separate cohorts: discovery (n = 93) and validation data set 1 (n = 78). The genome-wide methylation pattern was measured from whole blood using the Illumina 450K array. A second validation cohort, validation data set 2, consists of post-mortem brain biopsies from depressed adults (n = 58). We performed a MWAS by robust multiple linear regressions of methylation to a modified risk-score assessment of depression. Methylation levels of candidate CpG sites were correlated with expression levels of the associated gene in an independent cohort of 11 healthy volunteers. Results: The methylation state of two CpG sites reliably predicted ratings of depression in adolescents (cg13227623 and cg04102384) (p < 10E-06). Cohort validation analysis confirmed cg04102384 - located in the promoter region of microRNA 4646 (MIR4646) - to be hypomethylated in both validation data set 1 and validation data set 2 (p < 0.05). Cg04102384 was inversely correlated to expression levels of MIR4646-3p in healthy controls (p < 0.05). Limitations: MIR4646 was not differentially expressed in a subset of samples with adolescent depression measured by qRT-PCR measurements. Conclusion: We identify a specific MIR4646 associated epigenetic risk site to be associated with depression in adolescents. Cg04102384 putatively regulates gene expression of MIR4646-3p. Target gene prediction and gene set overrepresentation analysis revealed involvement of this miRNA in fatty acid elongation, a process related to omega-3 fatty acids, previously associated with depression.

  • Chao, Cai
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology.
    Shirazi Nasab, Fatemeh
    Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology.
    Linked Data Search and Browse Application2017Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    In order to achieve the ISO 26262 standard on the perspective of requirementstraceability, a huge volume of data has been converted into RDF format, and been stored in a Triple store. The need to have a web application to search and browse that RDF data has been raised. In order to build this application, several open source components such as Apache Solr, Apache Jena, Fuseki and Technologiessuch as Java, HTML, CSS, and Javascript have been used. The application has been evaluated with SUS method, an industry standard and reliable tool formeasuring the system usability, by six engineers from Scania, the evaluation resultin general is positive, five out of six participants successfully retrieved their desiredsearch results.

  • Haxsen, Sören
    Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences.
    SUITABILITY OF THE EMD-CONWX EUROPE MESOSCALE DATA FOR WIND RESOURCE ASSESSMENTS2017Independent thesis Advanced level (degree of Master (One Year)), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    The compilation of wind resource assessments and the implicit long-term correction ofwind measurements require comprehensive data sets. Commonly employed data sets forthis purpose are wind measurements from weather stations as well as SupervisoryControl and Data Acquisition (SCADA) data from existing wind farms. In addition,reanalysis data are a consistent data source. Reanalysis data are a combination ofmeteorological models with measurements of climatology parameters. To increase theperformance of reanalysis data the corresponding data sets are processed with mesoscalemodels. The present study determines the suitability of the readily accessible EMDConWxEurope Mesoscale Data (EMD-ConWx Data) for wind resource assessments.EMD-ConWx Data include hourly wind velocities at seven heights in the range of 10 mup to 200 m and have a spatial resolution of 3 x 3 km. EMD-ConWx Data are based onthe primary reanalysis data set ERA-Interim. The EMD-ConWx Data are compared toSonic Detecting and Ranging (Sodar) measurements at 22 sites in Germany regardingthe parameters wind speed, wind direction and wind speed frequency distribution. Inaddition, the statistical correlation (R) and linear regression (R²) are determined.It was found, that EMD-ConWx Data reveal a moderate accuracy for wind resourceassessments. The determined average wind speed bias of 1.02 m/s, the average rootmeans square error (RMSE) of 1.91 m/s, the average wind direction bias of -0.89° andthe monthly correlation indicate overall an adequate match with the Sodarmeasurements. However, these results entail considerable uncertainties and variances.To reduce these variances and the wind speed overestimation a height shift of 50 m forthe EMD-ConWx wind velocity heights is introduced. The comparison of the EMDConWxwind data at 50 m to Sodar measurements at 100 m leads to a significantreduction of the wind speed bias, but it improves neither the wind direction accuracy northe wind speed correlation. Overall, the EMD-ConWx Data are suitable for windresource assessments and the implicit long-term correction of wind data. The EMDConWxData with the height shift imply the advantage of a proper representation of thewind profile in relation to common reanalysis data, even at sites with complex terrain.

  • Hallgren, Fredrik
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Arts, Department of Archaeology and Ancient History.
    Sociala territorier och exogamirelationer i senmesolitisk tid: En diskussion utifrån boplatsen Pärlängsberget, Södermanland1996In: TOR, ISSN 0495-8772, Vol. 28, 5-28 p.Article in journal (Other academic)
  • Holmberg, Kristin
    Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences.
    Establishing a sustainable water supply in Chonyonyo, Karagwe, Tanzania2017Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    The small village Chonyonyo, in the district Karagwe in the northwest of Tanzania has a shortage of safe drinking water. Women and children spend several hours a day fetching water and gathering firewood to boil the water to make it more suitable for drinking. The need of new sustainable water supply solutions is fundamental for providing more people with safe drinking water.  

    Two water distribution alternatives was suggested by Engineers Without Borders and MAVUNO as possible solutions to supply the community with water.  Alternative 1 consisted of a 10 km distribution system from an already existing groundwater well at the MAVUNO office to Chonyonyo. Alternative 2 consisted of a 3.5 km distribution system from the most neighboring valley to Chonyonyo, where no groundwater well exists today. Both alternatives would be powered by solar panels and operated six hours a day. The most sustainable distribution solution was chosen by modeling the distribution alternatives in the modeling software EPANET. Input parameters to simulate the model were position, elevation and dimension of storage tanks and pipes. Other required input parameters were absolute roughness, water withdrawal, operation hours, description of the withdrawal pattern for the water outlet and other modelling conditions such as a suitable simulation time. The selection of water distribution system was based on minimum requirements of energy used for operation weighed with lowest possible water residence time in the storage tank.

    Water quality analyses of the raw water source for distribution alternative 1 were performed in order to classify the water and select suitable water treatment solutions. The analyses consisted of microbiological and metal/metalloid analyses, and measurements of EC and pH.

    The result of the simulation showed that neither of the distribution alternatives met all the pipe design criteria. The main reason is that the system can not be constantly operated. If this criterion is excluded the optimal solution is distribution alternative 1 with an outer pipe diameter of 110 mm throughout the whole distribution system and a water residence time in the storage tank of 57.1 hours.

    The water quality analysis showed that the ground water source for distribution alternative 1 was affected by surface water and is thus classified as unusable because of high levels of harmful bacteria and lead. The most suitable water treatment solution due to the aspects of sustainable water supply are the microbiological barriers ultrafiltration and UV-light in combination with a treatment method to remove lead from the water. 

  • Gräslund, Bo
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Arts, Department of Archaeology and Ancient History, Archaeology.
    Relativ datering.: Om kronologisk metod i nordisk arkeologi1974In: TOR, ISSN 91-7222-057-0, Vol. 16, 7-248 p.Article in journal (Other academic)
  • Public defence: 2017-12-01 13:00 Häggsalen, Ångström, Uppsala
    Nordh, Tim
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Structural Chemistry.
    A Quest for the Unseen: Surface Layer Formation on Li4Ti5O12 Li-Ion Battery Anodes2017Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    The electric vehicle itself today outlives its battery, necessitating battery replacement. Lithium titanium oxide (LTO) has, in this context, been suggested as a new anode material in heavy electric vehicle applications due to intrinsic properties regarding safety, lifetime and availability.

    The work presented here is focused on the LTO electrode/electrolyte interface. Photoelectron spectroscopy (PES) has been applied to determine how and if the usage of LTO could prevent extensive anode-side electrolyte decomposition and build-up of a surface layer. The presence of a solid electrolyte interphase (SEI) comprising LiF, carbonates and ether compounds was found in half-cells utilizing a standard ethylene:diethylcarbonate electrolyte with 1 M LiPF6. Via testing of symmetrical LTO-LTO cells, the stability of the formed SEI was put in to question. Moreover, the traditional polyvinylidene difluoride (PVdF) binder was replaced by more environmentally benign carboxylmethyl cellulose (CMC) and polyacrilic acid (PAA) binders in LTO electrodes, and it was found that CMC helped to form a more stable surface-layer that proved beneficial for long term cycling.

    Following the half-cell studies, full-cells were investigated to observe how different cathodes influence the SEI of LTO. The SEI in full-cells displayed characteristics similar to the half-cells, however, when utilizing a high voltage LiNi0.5Mn1.5O4 cathode, more electrolyte decomposition could be observed. Increasing the operational temperature of this battery cell generated even more degradation products on the LTO electrodes. Mn was also found on the anode when using Mn-based cathodes, however, it was found in its ionic state and did not significantly affect the composition or behavior of the observed SEI layer. Furthermore, by exchanging the electrolyte solvent for propylene carbonate, the thickness of the SEI increased, and by replacing the LiPF6 salt for LiBF4 the stability of the SEI improved. Thus is it demonstrated that such a passivation can be beneficial for the long-term surface stability of the electrode. These findings can therefore help prolong the lifetime of LTO-based battery chemistries.

    List of papers
    1. Depth profiling the solid electrolyte interphase on lithium titanate (Li4Ti5O12) using synchrotron-based photoelectron spectroscopy
    Open this publication in new window or tab >>Depth profiling the solid electrolyte interphase on lithium titanate (Li4Ti5O12) using synchrotron-based photoelectron spectroscopy
    2015 (English)In: Journal of Power Sources, ISSN 0378-7753, E-ISSN 1873-2755, Journal of Power Sources, Vol. 294, 173-179 p.Article in journal (Refereed) Published
    Abstract [en]

    The presence of a surface layer on lithium titanate (Li4Ti5O12, LTO) anodes, which has been a topic of debate in scientific literature, is here investigated with tunable high surface sensitive synchrotron-basedphotoelectron spectroscopy (PES) to obtain a reliable depth profile of the interphase. LijjLTO cells with electrolytes consisting of 1 M lithium hexafluorophosphate dissolved in ethylene carbonate:diethyl carbonate (LiPF6 in EC:DEC) were cycled in two different voltage windows of 1.0e2.0 V and 1.4e2.0 V. LTO electrodes were characterized after 5 and 100 cycles. Also the pristine electrode as such, and an electrode soaked in the electrolyte were analyzed by varying the photon energies enabling depth profiling of the outermost surface layer. The main components of the surface layer were found to be ethers, PeO containing compounds, and lithium fluoride.

    Keyword
    Li-ion batteries, LTO, PES, XPS, Surface layer, SEI
    National Category
    Materials Chemistry
    Research subject
    Chemistry with specialization in Materials Chemistry
    Identifiers
    urn:nbn:se:uu:diva-331106 (URN)10.1016/j.jpowsour.2015.06.038 (DOI)
    Available from: 2017-10-10 Created: 2017-10-10 Last updated: 2017-10-13
    2. Different Shades of Li4Ti5O12 Composites: The Impact of the Binder on Interface Layer Formation
    Open this publication in new window or tab >>Different Shades of Li4Ti5O12 Composites: The Impact of the Binder on Interface Layer Formation
    Show others...
    2017 (English)In: ChemElectroChem, 10.1002/celc.201700395- p.Article in journal (Refereed) Published
    Abstract [en]

    Replacing the traditional PVdF(-HFP) electrode binder by water-soluble alternatives can potentially render electrode fabrication more environmentally benign. Herein, the surface layer formation of stored and cycled samples of two water-based Li4Ti5O12 composites employing either poly(sodium acrylate) (PAA-Na) or sodium carboxymethyl cellulose (CMC-Na) as binders are studied by X-ray photoelectron spectroscopy. In all three formulations, the surface layer composition formed upon storage differed notably from the solid-electrolyte interphase (SEI) layer formed on cycled samples. The surface layer under open-circuit conditions seems to originate mostly from the electrolyte salt (LiPF6) degradation. The comparison with cycled samples after 10 and 100 cycles shows a continuous build-up of an SEI layer on PAA-Na and PVdF-HFP electrodes. In contrast, on CMC-Na containing electrodes the SEI composition remains nearly unchanged. The results correlate well with the electrochemical behavior.

    Keyword
    Li-ion batteries, LTO, binder, interface layer, photoelectron spectroscopy
    National Category
    Materials Chemistry
    Research subject
    Chemistry with specialization in Materials Chemistry
    Identifiers
    urn:nbn:se:uu:diva-331107 (URN)10.1002/celc.201700395 (DOI)
    Funder
    Swedish Energy AgencyStandUp
    Available from: 2017-10-10 Created: 2017-10-10 Last updated: 2017-10-13
    3. Manganese in the SEI layer of Li4Ti5O12 studied using combined NEXAFS and HAXPES techniques
    Open this publication in new window or tab >>Manganese in the SEI layer of Li4Ti5O12 studied using combined NEXAFS and HAXPES techniques
    Show others...
    2016 (English)In: The Journal of Physical Chemistry C, ISSN 1932-7447, E-ISSN 1932-7455, Vol. 120, no 6, 3206-3213 p.Article in journal (Refereed) Published
    Abstract [en]

    A combination of hard X-ray photoelectron spectroscopy (HAXPES) and near edge X-ray absorption fine structure (NEXAFS) are here used to investigate the presence and chemical state of crossover manganese deposited on Li-ion battery anodes. The synchrotron based experimental techniques-using HAXPES and NEXAFS analysis on the same sample in one analysis chamber-enabled us to acquire complementary sets of information. The Mn crossover and its influence on the anode interfacial chemistry has been a topic of controversy in the literature. Cells comprising lithium manganese oxide (LiMn2O4, LMO) cathodes and lithium titanate (Li4Ti5O12, LTO) anodes were investigated using LP40 (1 M LiPF6, EC:DEC 1:1) electrolyte. LTO electrodes at lithiated, delithiated, and open circuit voltage (OCV-stored) states were analyzed to investigate the potential dependency of the manganese oxidation state. It was primarily found that a solid surface layer was formed on the LTO electrode and that this layer contains deposited Mn from the cathode. The results revealed that manganese is present in the ionic state, independent of the lithiation of the LTO electrode. The chemical environment of the deposited manganese could not be assigned to simple compounds such as fluorides or oxides, indicating that the state of manganese is in a more complex form.

    National Category
    Other Chemical Engineering
    Research subject
    Chemistry with specialization in Materials Chemistry
    Identifiers
    urn:nbn:se:uu:diva-267788 (URN)10.1021/acs.jpcc.5b11756 (DOI)000370678700012 ()
    Funder
    Swedish Energy Agency
    Available from: 2015-11-26 Created: 2015-11-26 Last updated: 2017-10-13Bibliographically approved
    4. Understanding the capacity loss in LNMO-LTO lithium-ion cells at ambient and elevated temperatures
    Open this publication in new window or tab >>Understanding the capacity loss in LNMO-LTO lithium-ion cells at ambient and elevated temperatures
    Show others...
    (English)Manuscript (preprint) (Other academic)
    Keyword
    High voltage spinel, cross-talk, electrode interactions, LNMO-LTO, Mn dissolution
    National Category
    Materials Chemistry
    Research subject
    Chemistry with specialization in Materials Chemistry
    Identifiers
    urn:nbn:se:uu:diva-331117 (URN)
    Available from: 2017-10-10 Created: 2017-10-10 Last updated: 2017-10-13
    5. Surface Layer Formation on Li4Ti5O12 Electrodes in Li-ion Cells with Propylene Carbonate-Based Electrolyte
    Open this publication in new window or tab >>Surface Layer Formation on Li4Ti5O12 Electrodes in Li-ion Cells with Propylene Carbonate-Based Electrolyte
    (English)Manuscript (preprint) (Other academic)
    Abstract [en]

    The increasing usage of lithium titanate (Li4Ti5O12; LTO) as anode material in Li-ion batteries creates challenges and possibilities for the electrolytes. Traditional Li-ion battery electrolytes are tailored primarily for chemistries that use graphite as anode, and are therefore not optimized for LTO electrodes. In this study, propylene carbonate is, together with LiPF6 salt, investigated as an alternative electrolyte system in such batteries. The LTO surface is investigated with photoelectron spectroscopy after the formation cycle and after 10 cycles to characterize the decomposition products. The results show the presence of a surface layer formed on the LTO electrode irrespective of counter electrode used, but with varying thickness. Contrary to conventional ethylene carbonate based electrolytes, no manganese crossover could be observed when using either lithium manganese oxide or lithium nickel manganese oxide/lithium cobalt oxide composite as cathodes in LTO cells.

    Keyword
    Li-ion battery, LTO anode, Interface layer, Propylene carbonate, Photoelectron spectroscopy
    National Category
    Materials Chemistry
    Research subject
    Chemistry with specialization in Materials Chemistry
    Identifiers
    urn:nbn:se:uu:diva-331116 (URN)
    Available from: 2017-10-10 Created: 2017-10-10 Last updated: 2017-10-13
  • Wikén, Moa
    Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences.
    Optimering av blåsmaskinstyrning på Bromma reningsverk2017Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    The aeration step is the most energy consuming process in the wastewater treatment plant, i.e. making the energy usage in this step more efficient is of great concern both in Sweden and world wide. There are some examples of previous studies investigating ways to make the ae- ration control more efficient, though most of these have focused on for example ammonium feedback control rather than blower control which is the main focus of this study.

    Bromma wastewater treatment plant installed three new blowers in december 2016 functioning as suppliers of air to the aeration step in the bioreactor. Since there are still three old blowers operating at the WWTP, there is a need to control two different types of blowers efficiently. This is challenging since the two blower types have different capacities, efficiency and maxi- mum/minimum air flows and a control strategy that optimizes the operation of these two blower types combined needs to take this into account.

    Operational data for the blowers was used to generate a second grade polynome for each blower type which gives the efficiency as a function of produced air flow. These polynomes were further utilized to build a script in MATLAB that chooses the most efficient number of blowers operating and at what air flows for a certain total aeration need. The least square method was used to calibrate a model for calculating energy consumption for the simulated control strate- gy. Calculations for energy consumption from the current control strategy and another strategy proposed by the blower deliverer Sulzer were made in order to decide whether the optimized control strategy was an improvement or not.

    The simulation results and the calculations for energy consumption indicates that the current control strategy at Bromma WWTP is almost as effective as the optimized control strategy. Both strategies sets the new blowers at the first places in the queue. The optimized strategy appears to be more efficient for flows that requires both new and old blowers operating. From the calculations of energy consumption, it was shown that the control strategy from Sulzer de- mands an energy consumption on levels in between the one for the current control strategy and the optimized control strategy. 

  • Public defence: 2017-12-01 13:15 2446, Uppsala
    Peters, Anne-Kathrin
    Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Computer Systems.
    Learning Computing at University: Participation and Identity: A Longitudinal Study2017Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Computing education has struggled with student engagement and diversity in the student population for a long time. Research in science, technology, engineering, and mathematics (STEM) education suggests that taking a social, long-term perspective on learning is a fruitful approach to resolving some of these persistent challenges.

    A longitudinal study has been conducted, following students from two computing study programmes (CS/IT) over a three-year period. The students reflected on their experiences with CS/IT in a series of interviews. Drawing on social identity theory, the analysis has focused on describing participation in CS/IT, doing, thinking, feeling in relation to CS/IT, as negotiated among different people.

    Phenomenographic analysis yields an outcome space that describes increasingly broad ways in which the students experience participation in CS/IT over the years. Two further outcome spaces provide nuanced insights into experiences that are of increasing relevance as the students advance in their studies; participation as problem solving and problem solving for others. Problem solving defined as solving difficult (technical) problems seems predominate in the learning environment. Problem solving for others brings the user into perspective, but first in the human computer interaction (HCI) course in year three. Students react with scepticism to HCI, excluding HCI from computing, some are students who commenced their studies with broader interests in computing.

    Demonstrating (technical) problem solving competence is the most vital indicator competence in the two study programmes and the students adapt their reflections on who they are as computing students and professionals accordingly. People showing broader interests in computing risk being marginalised. I identify a gap between conceptions of computing as interdisciplinary and important for society and constructions of computing as technical. Closing the gap could improve retention and diversity, and result in graduates that are better prepared to contribute to societal development.

    List of papers
    1. Students' experiences and attitudes towards learning Computer Science
    Open this publication in new window or tab >>Students' experiences and attitudes towards learning Computer Science
    2012 (English)In: Proc. 42nd ASEE/IEEE Frontiers in Education Conference, Piscataway, NJ: IEEE , 2012, 88-93 p.Conference paper, Published paper (Refereed)
    Place, publisher, year, edition, pages
    Piscataway, NJ: IEEE, 2012
    National Category
    Computer Science Educational Sciences
    Identifiers
    urn:nbn:se:uu:diva-184605 (URN)10.1109/FIE.2012.6462238 (DOI)000356489200033 ()978-1-4673-1353-7 (ISBN)
    Available from: 2012-10-06 Created: 2012-11-09 Last updated: 2017-10-16Bibliographically approved
    2. Engagement in Computer Science and IT — What!: A matter of identity?
    Open this publication in new window or tab >>Engagement in Computer Science and IT — What!: A matter of identity?
    2013 (English)In: Proc. 1st International Conference on Learning and Teaching in Computing and Engineering, Los Alamitos, CA: IEEE Computer Society, 2013, 114-121 p.Conference paper, Published paper (Refereed)
    Place, publisher, year, edition, pages
    Los Alamitos, CA: IEEE Computer Society, 2013
    National Category
    Computer Science Educational Sciences
    Identifiers
    urn:nbn:se:uu:diva-202686 (URN)10.1109/LaTiCE.2013.42 (DOI)000324484000016 ()978-1-4673-5627-5 (ISBN)
    Conference
    LaTiCE 2013
    Available from: 2013-06-22 Created: 2013-06-25 Last updated: 2017-10-16Bibliographically approved
    3. First year Computer Science and IT students' experience of participation in the discipline
    Open this publication in new window or tab >>First year Computer Science and IT students' experience of participation in the discipline
    2014 (English)In: Proc. 2nd International Conference on Learning and Teaching in Computing and Engineering, Los Alamitos, CA: IEEE Computer Society, 2014, 1-8 p.Conference paper, Published paper (Refereed)
    Place, publisher, year, edition, pages
    Los Alamitos, CA: IEEE Computer Society, 2014
    National Category
    Computer Science Educational Sciences
    Identifiers
    urn:nbn:se:uu:diva-224653 (URN)10.1109/LaTiCE.2014.9 (DOI)000355978500001 ()978-1-4799-3591-8 (ISBN)
    Conference
    LaTiCE 2014
    Available from: 2014-06-10 Created: 2014-05-16 Last updated: 2017-10-16Bibliographically approved
    4. Second year Computer Science and IT students' experience of participation in the discipline
    Open this publication in new window or tab >>Second year Computer Science and IT students' experience of participation in the discipline
    2015 (English)In: Proc. 15th International Conference on Computing Education Research: Koli Calling, New York: ACM Press, 2015, 68-76 p.Conference paper, Published paper (Refereed)
    Place, publisher, year, edition, pages
    New York: ACM Press, 2015
    National Category
    Computer Science Educational Sciences
    Identifiers
    urn:nbn:se:uu:diva-269178 (URN)10.1145/2828959.2828962 (DOI)978-1-4503-4020-5 (ISBN)
    Available from: 2015-11-21 Created: 2015-12-14 Last updated: 2017-10-16Bibliographically approved
    5. Students' experience of participation in a discipline: A longitudinal study of computer science and IT engineering students
    Open this publication in new window or tab >>Students' experience of participation in a discipline: A longitudinal study of computer science and IT engineering students
    2017 (English)In: ACM Transactions on Computing Education, ISSN 1946-6226, E-ISSN 1946-6226Article in journal (Refereed) Accepted
    National Category
    Computer and Information Science Educational Sciences
    Identifiers
    urn:nbn:se:uu:diva-331401 (URN)
    Available from: 2017-10-13 Created: 2017-10-13 Last updated: 2017-10-24Bibliographically approved
  • Poelzl, Gerhard
    et al.
    Univ Klin Innsbruck, Innsbruck, Austria..
    Altenberger, Johann
    Rehabil Zentrum Grossgmain, Salzburg, Austria..
    Baholli, Loant
    Klinikum Dortmund Mitte, Dortmund, Germany..
    Beltran, Paola
    HM Broggi, Barcelona, Spain..
    Borbely, Attila
    Univ Debrecen, Fac Med, Div Clin Physiol, Debrecen, Hungary..
    Comin-Colet, Josep
    Univ Hosp Bellvitge, Barcelona, Spain..
    Delgado, Juan F.
    Hosp 12 Octubre, Madrid, Spain..
    Fedele, Francesco
    Sapienza Univ, Policlin Umberto 1, Rome, Italy..
    Fontana, Antonella
    Orion Pharma Srl, Milan, Italy..
    Fruhwald, Friedrich
    Med Univ Klin, Graz, Austria..
    Giamouzis, Gregory
    Univ Thessaly, Larissa Univ Hosp, Larisa, Greece..
    Giannakoulas, George
    Aristotle Univ Thessaloniki, Thessaloniki, Greece..
    Garcia-Gonzalez, Martin J.
    H La Laguna, Tenerife, Spain..
    Gustafsson, Finn
    Rigshosp, Copenhagen, Denmark..
    Kaikkonen, Kari
    Oulu Univ Hosp, Oulu, Finland..
    Kivikko, Matti
    Orion Pharma, Espoo, Finland..
    Kubica, Jacek
    Nicolaus Copernicus Univ, Coll Med, Bydgoszcz, Poland..
    von Lewinski, Dirk
    Med Univ Klin, Graz, Austria..
    Lofman, Ida
    Karolinska Univ Sjukhus Huddinge, Huddinge, Sweden..
    Malfatto, Gabriella
    Ist Auxol Italiano, Milan, Italy..
    Manito, Nicolas
    Univ Hosp Bellvitge, Barcelona, Spain..
    Martinez-Selles, Martin
    H Gregorio Maranon, Madrid, Spain..
    Masip, Josep
    HM Broggi, Barcelona, Spain..
    Merkely, Bela
    Semmelweis Univ, Heart & Vasc Ctr, Budapest, Hungary..
    Morandi, Fabrizio
    Circolo Hosp & Macchi Fdn, Varese, Italy..
    Molgaard, Henning
    Aarhus Univ Hosp, Skejby, Denmark..
    Oliva, Fabrizio
    Osped Niguarda Ca Granda, Milan, Italy..
    Pantev, Emil
    Helsingborgs Iasarett, Helsingborg, Sweden..
    Papp, Zoltan
    Univ Debrecen, Fac Med, Div Clin Physiol, Debrecen, Hungary..
    Perna, Gian Piero
    Osped Riuniti, Dipartimento Sci Cardiol Med Chirurg, Ancona, Italy..
    Pfister, Roman
    Univ Cologne, Herzzentrum, Klin Innere Med 3, Cologne, Germany..
    Piazza, Vito
    Azienda Osped San Camillo Forlanini, Rome, Italy..
    Bover, Ramon
    H Clin San Carlos, Madrid, Spain..
    Rangel-Sousa, Diego
    H Virgen Rocio, Seville, Spain..
    Recio-Mayoral, Alejandro
    Hosp Univ Virgen Macarena, Seville, Spain..
    Reinecke, Alexander
    Uni Klinikum Schleswig Holstein, Kiel, Germany..
    Rieth, Andreas
    Kerckhoff Klin, Bad Nauheim, Germany..
    Sarapohja, Toni
    Orion Pharma, Espoo, Finland..
    Schmidt, Gunter
    CHARITE Univ Med Berlin, Berlin, Germany..
    Seidel, Mirko
    Unfallkrankenhaus Berlin, Innere Med Klin, Berlin, Germany..
    Stoerk, Stefan
    Univ Wurzburg, Comprehens Heart Failure Ctr, Wurzburg, Germany.;Univ Hosp, Wurzburg, Germany..
    Vrtovec, Bojan
    Univ Clin Ctr, Ljubljana, Slovenia..
    Wikström, Gerhard
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Cardiology.
    Yerly, Patrik
    CHU Vaudois, Lausanne, Switzerland..
    Pollesello, Piero
    Orion Pharma, Espoo, Finland..
    Repetitive use of levosimendan in advanced heart failure: need for stronger evidence in a field in dire need of a useful therapy2017In: International Journal of Cardiology, ISSN 0167-5273, E-ISSN 1874-1754, Vol. 243, 389-395 p.Article in journal (Refereed)
    Abstract [en]

    Patients in the latest stages of heart failure are severely compromised, with poor quality of life and frequent hospitalizations. Heart transplantation and left ventricular assist device implantation are viable options only for a minority, and intermittent or continuous infusions of positive inotropes may be needed as a bridge therapy or as a symptomatic approach. In these settings, levosimendan has potential advantages over conventional inotropes (catecholamines and phosphodiesterase inhibitors), such as sustained effects after initial infusion, synergy with beta-blockers, and no increase in oxygen consumption. Levosimendan has been suggested as a treatment that reduces re-hospitalization and improves quality of life. However, previous clinical studies of intermittent infusions of levosimendan were not powered to show statistical significance on key outcome parameters. A panel of 45 expert clinicians from 12 European countries met in Rome on November 24-25, 2016 to review the literature and envision an appropriately designed clinical trial addressing these needs. In the earlier FIGHT trial (daily subcutaneous injection of liraglutide in heart failure patients with reduced ejection fraction) a composite Global Rank Score was used as primary end-point where death, re-hospitalization, and change in N-terminalprohormone-brain natriuretic peptide level were considered in a hierarchical order. In the present study, we tested the same end-point post hoc in the PERSIST and LEVOREP trials on oral and repeated i.v. levosimendan, respectively, and demonstrated superiority of levosimendan treatment vs placebo. The use of the same composite end-point in a properly powered study on repetitive levosimendan in advanced heart failure is strongly advocated.

  • Public defence: 2017-12-01 09:15 Sal IX, Uppsala
    Fängström, Karin
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Social Medicine.
    ‘I don’t even remember anything’: Optimising the choice of method when interviewing preschoolers2017Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    There is increasing need and demand in various contexts to take children’s perspectives into account, including the views and opinions of the youngest children. However, listening to the voices of children is a challenging and complex task, and the field is normatively loaded. There is thus a growing need for valid and reliable methods and techniques that aid children to verbalise their experiences. The overall aim of this thesis was to examine the ability of the In My Shoes computer assisted interview and a Standard verbal interview to elicit accurate information and evaluative content, when used with preschool-aged children and determine their suitability in relation to situationally shy children.

    Our studies show that the two interview methods, in general, provided equally accurate and complete statements. In addition, the IMS interview can be a more useful and suitable tool during the rapport phase with situationally shy children compared to the Standard verbal method. For non-shy children, the interview methods were equally adequate. In relation to evaluative information, the recommended open-ended questions in the Standard verbal interview were insufficient. Children appeared to need evaluative questions in order to provide evaluative content. Examining the ability of IMS to elicit subjective experiences showed that using IMS aided children to provide detailed and varied descriptions of emotions, somatic experiences, and objects such as toys.  

    Thus, when choosing the optimal child interview method, there are several aspects that need to be considered, including the degree to which children’s statements need to be accurate and complete and/or contain evaluative information and the child’s level of shyness. These studies have increased the number of evaluated methods for interviewing children and contributed to new knowledge about the challenging task of optimising the choice of method for interviewing preschoolers.

    List of papers
    1. In My Shoes - Validation of a computer assisted approach for interviewing children
    Open this publication in new window or tab >>In My Shoes - Validation of a computer assisted approach for interviewing children
    Show others...
    2016 (English)In: Child Abuse & Neglect: The International Journal, ISSN 0145-2134, Vol. 58, 160-172 p.Article in journal (Refereed) Published
    Abstract [en]

    Interviewing young children presents a challenge because they tend to provide incomplete accounts and are easily misled. Therefore there is a need for techniques to improve young children's recall, while maintaining accuracy and increasing completeness. The computer-assisted interview In My Shoes (IMS) is an aid that potentially offers a way for young children to provide accounts of their experiences. This study examined the validity of IMS, by comparing it with a forensic best practice interview approach using a real life clinical situation to ensure high ecological validity. Children were randomly assigned to either method and both accuracy and completeness of statements made by 4- and 5-year-olds (N = 54) regarding a video-documented health check-up were assessed. The In My Shoes interviews were as good as best practice interviews on all accuracy measures for both age groups, except for object accuracy that was better in the forensic interview condition. Events description completeness was similar in both interview conditions; however, IMS interviews generated more complete statements about people present at the visit. The findings suggest that the IMS approach yields comparable results to a best practice interview, and it can be used as an alternative aid in child interviews.

    Keyword
    Interviewing aid, Child, Computer-assisted interview, Validity, In My Shoes
    National Category
    Public Health, Global Health, Social Medicine and Epidemiology
    Research subject
    Social Medicine; Psychology
    Identifiers
    urn:nbn:se:uu:diva-303099 (URN)10.1016/j.chiabu.2016.06.022 (DOI)000381241400016 ()27394051 (PubMedID)
    Funder
    VINNOVA, 259-2012-68Forte, Swedish Research Council for Health, Working Life and Welfare, 259-2012-68
    Note

    Forskningsfinansiär: Allmänna Barnhuset, FB13-0014

    Available from: 2016-09-28 Created: 2016-09-15 Last updated: 2017-10-13
    2. The computer-assisted interview In My Shoes can benefit shy preschool children's communication
    Open this publication in new window or tab >>The computer-assisted interview In My Shoes can benefit shy preschool children's communication
    2017 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 8, e0182978Article in journal (Refereed) Published
    Abstract [en]

    Interviewing children is a cognitively, socially, and emotionally challenging situation, especially for young and shy children. Thus, finding methods that aid rapport and increase these children's communication is important. The present study investigated whether children's verbal and non-verbal communicative behavior developed differently during the rapport phase, depending on whether children were situationally shy or not, and whether the interview was conducted using the computer-assisted interview In My Shoes (IMS) or a Standard verbal interview. The sample consisted of 60 children aged 4 to 5-years-old. The results showed that for the shy children in the IMS group their talkativeness increased and their answer latency decreased including the amount of encouragement the child needed to talk, while no changes were observed for the shy children in the Standard verbal interview group. There were no significant differences in the non-verbal behavior for the shy children regardless of the interview method used. For the non-shy children, overall, the interview method did not affect either the verbal or the non-verbal outcomes. Our findings indicate that IMS can be a useful tool during the rapport-building phase with shy children as it helps these children to improve their verbal communication.

    Keyword
    child, interview, communication, In My Shoes
    National Category
    Public Health, Global Health, Social Medicine and Epidemiology
    Research subject
    Psychology
    Identifiers
    urn:nbn:se:uu:diva-328584 (URN)10.1371/journal.pone.0182978 (DOI)000407550500034 ()28813534 (PubMedID)
    Funder
    Swedish Research Council, 259-2012-68Swedish Research Council FormasVINNOVA
    Available from: 2017-08-28 Created: 2017-08-28 Last updated: 2017-11-14Bibliographically approved
    3. “And they gave me a shot, it really hurt” – Evaluative content in investigative interviews with young children
    Open this publication in new window or tab >>“And they gave me a shot, it really hurt” – Evaluative content in investigative interviews with young children
    Show others...
    2017 (English)In: Children and youth services review, ISSN 0190-7409, E-ISSN 1873-7765, Vol. 82, 434-443 p.Article in journal (Refereed) Published
    Abstract [en]

    Research is scarce on the suitability of the evidence-based components of child investigative interviews when used in non-forensic contexts such as social work or school, particularly in relation to children’s reports on emotional content.

    This explorative study investigated to what extent a structured forensic interview protocol aids children in verbalizing negative emotional experiences of distress or discomfort. To do this we assessed and compared children’s displayed distress or discomfort during a video-recorded health visit with the verbalized distress or discomfort in interviews 2-4 weeks later about this visit. The children, aged 4 and 5 years (N = 26), were interviewed with a forensic interview protocol. Children’s statements regarding distress and discomfort and the interviewer questions preceding these statements were analyzed qualitatively.

    The results showed that 46% of the 4-year-olds and 39 % of the 5-year-olds displayed discomfort or distress during their health visit. In the interviews, open-ended questions were posed to all children, however, these questions were sufficient to aid only some children (n = 6) to share evaluative content. None of the children who displayed distress or discomfort during the visit verbalized such experiences after an invitation only. Most children who described experiences of distress or discomfort did so in relation to evaluative questions.

    The results suggest that more research is warranted to investigate exactly how and when evaluative questions should be posed and whether this differs depending on severity of experience or the child’s age. The need for protocol development and its suitability when used in other fields of practice is discussed.

    Keyword
    investigative interviews, evaluative, emotion, question, distress
    National Category
    Public Health, Global Health, Social Medicine and Epidemiology
    Research subject
    Psychology
    Identifiers
    urn:nbn:se:uu:diva-328585 (URN)10.1016/j.childyouth.2017.10.017 (DOI)
    Funder
    Swedish Research Council, 259-2012-68
    Note

    Forskningsfinansiär: Allmänna Barnhuset, FB13-0014

    Available from: 2017-10-11 Created: 2017-10-11 Last updated: 2017-10-19
    4. 'I felt a little bubbly in my tummy': Eliciting pre-schoolers' accounts of their health visit using a computer-assisted interview method.
    Open this publication in new window or tab >>'I felt a little bubbly in my tummy': Eliciting pre-schoolers' accounts of their health visit using a computer-assisted interview method.
    Show others...
    2016 (English)In: Child Care Health and Development, ISSN 0305-1862, E-ISSN 1365-2214, Vol. 42, no 1, 87-97 p.Article in journal (Refereed) Published
    Abstract [en]

    BACKGROUND: In the health care services, children's rights to participate in all matters that concern them are considered important. However, in practice this can be challenging with young children. In My Shoes (IMS) is a computer-assisted interview tool developed to help children talk about their experiences. The aim of the study was to evaluate the IMS' ability to elicit pre-schoolers' subjective experiences and accurate accounts of a routine health visit as well as the children's engagement in the interview process.

    METHODS: Interviews were conducted with 23 children aged 4-5 years, 2-4 weeks after their health visit. The interviews were transcribed verbatim and analysed using a method inspired by Content Analysis to evaluate IMS's ability to elicit accounts about subjective experiences. Accurate accounts were assessed by comparing the transcribed interviews with the filmed visits at the child health centre. The children's engagement was defined by the completion and length of the interviews, and the children's interaction with the software.

    RESULTS: All children gave accounts about their subjective experiences, such as their emotional state during the visit, available toys or rewards they received. All children related to the correct event, they all named at least one person who was present and 87% correctly named at least one examination procedure. The majority of children (91%) completed the interview, which lasted 17-39 min (M = 24), and 96% interacted with the IMS software.

    CONCLUSIONS: IMS was feasible to help children describe their health care experiences, in both detail and depth. The children interacted with the software and maintained their interest for an extended period of time.

    Keyword
    child interview; child public health; children's rights; children's views; computer; qualitative research methods
    National Category
    Pediatrics
    Identifiers
    urn:nbn:se:uu:diva-269207 (URN)10.1111/cch.12293 (DOI)000367930300011 ()26564782 (PubMedID)
    Funder
    VINNOVA, 259-2012-68Swedish Research Council, 259-2012-68Forte, Swedish Research Council for Health, Working Life and Welfare, 259-2012-68
    Note

    Forskningsfinansiär: Allmänna Barnhuset, FB13-0014

    Available from: 2015-12-14 Created: 2015-12-14 Last updated: 2017-10-13
  • Hjelm, Annica
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Arts, Department of ALM.
    Att knyta näven i fickan? : En studie av socialdemokratiska och kommunistiska 1 maj-affischer 1922-1948 utifrån begreppet vredeskultur 2017Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    This study is intended to illustrate how the reformist and revolutionary directions in the labor movement are manifested and visualized in the empirical material with regard to angry culture, thus contributing to an understanding of the informative importance of the image. It is clear that the investigated material in the form of social democratic and communist 1 May posters from the period 1922-1948 reflects its time (the interwar period to the post-war era) with regard to angry culture.  It is not the aesthetic aspect that is central, but the historical and political perspective in terms of information transmission. This study focuses on how to understand a historical period through an image material. The study aims to answer the question of what kind of information is communicated built on an analysis based on a combination of semiotics and hegemony analysis of the empirical material.

    Using the semiotic concepts denotation and connotation, the posters are analyzed in detail in the image analysis in combination with Stuart Hall's three hypothetical positions (codes), Antonio Gramsci’s hegemony concept, primarily in the form of “war of position” and “historic blocs”, is used to understand the historical period investigated. This method triangulation increases the credibility of the results.

    As far as the study and its results are concerned, it can be noted that the social democratic 1 May posters before 1936 (Social Democrats’ power access) with regard to “sublimed wrath” mainly represent consensus across class boundaries. The communist 1 May posters from that period, however, primarily represent “class war” and stand for “open anger”. After the Social Democrats’ access to power, it is clear that they constitute a historic bloc and that the communists are forced to adopt a more defensive approach, pronounced in drained paroles with a vague content.

    The conclusion with regard to the visual transmission of political and historical information and messages regarding social democratic and communistic 1 May posters from 1922-1948, based on socialist angry cultures is that the period between the wars was characterized by class struggle versus consensus, World War ll reduced everything to “hold together” and post-war time represents the starting point for the welfare state, as a successful consequence of the social democratic “dignity project”.

     

  • Johannesson, Elsa
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Arts, Department of ALM.
    Klassiker på barn- och ungdomsbiblioteket: Bibliotekariens arbete med barn- och ungdomslitteraturens klassiker2017Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    Introduction. This empirical study investigates the attitudes of Swedish public librarians who work in the children’s and/or young adult departments, towards children’s literary classics. This is achieved by examining work with the Classics shelf, a genre classification located in the children’s or young adult section.

    Method. The empirical material consists of transcripts from interviews with seven children’s and young adult public librarians in four Swedish municipalities, the Classics shelves and the libraries’ policy documents.

    Analysis. The Classics shelves’ genre definition, target group and location were investigated and compared to the libraries’ policy documents. Interview transcripts were examined in regard to the explicit contents of the participants’ statements and divided in themes by perceptions of value, function and use of the literature in a library context. Magnus Persson’s concept of myths describing naturalised perceptions of literature was used to interpret attitudes. A concluding analysis was conducted using the discourse theories of Michel Foucault, Ernesto Laclau and Chantal Mouffe.

    Results. The analysis indicated both shared and conflicting attitudes to children’s and young adult literary classics, with perceptions predominantly taken for granted. Three coexisting discourses were identified: the Reading experience discourse, the Durability discourse and the Fresh discourse. These are mainly tied to different tasks and influenced by the librarians’ preconceived notions, workplace management and external influences such as media debates, with the user perspective as a prerequisite.

    Conclusion. The results show that the librarian accommodates contradictory and ambivalent views of children’s and young adult literary classics in the library. Literature is mediated indirectly and directly, and expresses the librarian’s personal experience rather than a professional identity.

    This is a two years master’s thesis in Library and Information Science.

  • Wiezell, Astrid
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Arts, Department of ALM.
    Äldre användare på Stockholms stadsbibliotek: en nutida kvalitativ intervjustudie2017Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    The aim of this study is to gain deeper understanding of how older patrons (65 years of age and above) experien- ce the Stockholm Public Library: how the older patrons use the library, experience information about events and programs; experiences in contact with librarians; how user-friendly they find the library and what might attract or detract visits. The study consists of qualitative interviews with five older patrons of the Stockholm Public Lib- rary who were approached during a library visit. The study also contains interviews with two librarians from the library. The interviews have been transcribed and the results are organized into six areas: using the library and its services; digital aspects of the library; contact with librarians; the library space in a user-friendly context; the cultural value of the library building; library programmes; events and programs.

    The theoretical framework of the study is that of sociologist and philosopher Zygmunt Bauman and his ide- as about the individualised society and consumerism. Bauman's thoughts on the individualised society where on- ly the desired consumers are welcome even in public spaces have also been included. These theories are combi- ned with research on how society views aging and older people, international studies and studies on older pa- trons and libraries in Sweden.

    This study finds that a main issue in accessing the library's services and material is how they are placed: shelves that are too high or low to reach, and problems with finding material without help. The patrons are drawn to places where books are exposed, whether on purpose or not. The study concludes with the suggestion of crea- ting national guidelines for how libraries can make their spaces more welcoming to older patrons, such as ex- isting guidelines in the U.S. And Australia. Previous research shows the mere existance of guidelines may high- light the issue in itself and create a helpful start for libraries wanting to accommodate older patrons better.

  • Schwager, Evelyn E.
    et al.
    Oxford Brookes Univ, Dept Biol & Med Sci, Gipsy Lane, Oxford OX3 0BP, England.;Univ Massachusetts Lowell, Dept Biol Sci, 198 Riverside St, Lowell, MA 01854 USA..
    Sharma, Prashant P.
    Univ Wisconsin Madison, Dept Zool, 430 Lincoln Dr, Madison, WI 53706 USA..
    Clarke, Thomas
    Washington & Lee Univ, Dept Biol, 204 West Washington St, Lexington, VA 24450 USA.;Univ Calif Riverside, Dept Biol, Riverside, CA 92521 USA.;J Craig Venter Inst, 9714 Med Ctr Dr, Rockville, MD 20850 USA..
    Leite, Daniel J.
    Oxford Brookes Univ, Dept Biol & Med Sci, Gipsy Lane, Oxford OX3 0BP, England..
    Wierschin, Torsten
    Ernst Moritz Arndt Univ Greifswald, Inst Math & Comp Sci, Walther Rathenau Str 47, D-17487 Greifswald, Germany..
    Pechmann, Matthias
    Univ Goettingen, Johann Friedrich Blumenbach Inst Zool & Anthropol, Dept Dev Biol, GZMB Ernst Caspari Haus, Justus von Liebig Weg 11, D-37077 Gottingen, Germany.;Univ Cologne, Cologne Bioctr, Inst Zool, Dept Dev Biol, Zuelpicher Str 47b, D-50674 Cologne, Germany..
    Akiyama-Oda, Yasuko
    JT Biohist Res Hall, 1-1 Murasaki Cho, Takatsuki, Osaka 5691125, Japan.;Osaka Med Coll, Takatsuki, Osaka, Japan..
    Esposito, Lauren
    Calif Acad Sci, Inst Biodivers Sci & Sustainabil, 55 Mus Concourse Dr, San Francisco, CA 94118 USA..
    Bechsgaard, Jesper
    Aarhus Univ, Dept Biosci, Ny Munkegade 116,Bldg 1540, DK-8000 Aarhus C, Denmark..
    Bilde, Trine
    Aarhus Univ, Dept Biosci, Ny Munkegade 116,Bldg 1540, DK-8000 Aarhus C, Denmark..
    Buffry, Alexandra D.
    Oxford Brookes Univ, Dept Biol & Med Sci, Gipsy Lane, Oxford OX3 0BP, England..
    Chao, Hsu
    Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA..
    Dinh, Huyen
    Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA..
    Doddapaneni, HarshaVardhan
    Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA..
    Dugan, Shannon
    Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA..
    Eibner, Cornelius
    Friedrich Schiller Univ Jena, Dept Genet, Philosophenweg 12, D-07743 Jena, Germany..
    Extavour, Cassandra G.
    Harvard Univ, Dept Organism & Evolutionary Biol, 16 Divin Ave, Cambridge, MA 02138 USA..
    Funch, Peter
    Aarhus Univ, Dept Biosci, Ny Munkegade 116,Bldg 1540, DK-8000 Aarhus C, Denmark..
    Garb, Jessica
    Univ Massachusetts Lowell, Dept Biol Sci, 198 Riverside St, Lowell, MA 01854 USA..
    Gonzalez, Luis B.
    Oxford Brookes Univ, Dept Biol & Med Sci, Gipsy Lane, Oxford OX3 0BP, England..
    Gonzalez, Vanessa L.
    Smithsonian Natl Museum Nat Hist, MRC 163,POB 37012, Washington, DC 20013 USA..
    Griffiths-Jones, Sam
    Univ Manchester, Fac Biol Med & Hlth, D1416 Michael Smith Bldg,Oxford Rd, Manchester M13 9PT, Lancs, England..
    Han, Yi
    Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA..
    Hayashi, Cheryl
    Univ Calif Riverside, Dept Biol, Riverside, CA 92521 USA.;Amer Museum Nat Hist, Div Invertebrate Zool, New York, NY 10024 USA..
    Hilbrant, Maarten
    Oxford Brookes Univ, Dept Biol & Med Sci, Gipsy Lane, Oxford OX3 0BP, England.;Univ Cologne, Cologne Bioctr, Inst Zool, Dept Dev Biol, Zuelpicher Str 47b, D-50674 Cologne, Germany..
    Hughes, Daniel S. T.
    Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA..
    Janssen, Ralf
    Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences, Palaeobiology.
    Lee, Sandra L.
    Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA..
    Maeso, Ignacio
    Univ Pablo de Olavide, CABD, CSIC, Seville, Spain..
    Murali, Shwetha C.
    Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA..
    Muzny, Donna M.
    Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA..
    da Fonseca, Rodrigo Nunes
    Univ Fed Rio de Janeiro, Nucleo Ecol & Desenvolvimento SocioAmbiental Maca, Campus Macae, BR-27941222 Rio de Janeiro, Brazil..
    Paese, Christian L. B.
    Oxford Brookes Univ, Dept Biol & Med Sci, Gipsy Lane, Oxford OX3 0BP, England..
    Qu, Jiaxin
    Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA..
    Ronshaugen, Matthew
    Univ Manchester, Fac Biol Med & Hlth, D1416 Michael Smith Bldg,Oxford Rd, Manchester M13 9PT, Lancs, England..
    Schomburg, Christoph
    Univ Goettingen, Johann Friedrich Blumenbach Inst Zool & Anthropol, Dept Dev Biol, GZMB Ernst Caspari Haus, Justus von Liebig Weg 11, D-37077 Gottingen, Germany..
    Schönauer, Anna
    Oxford Brookes Univ, Dept Biol & Med Sci, Gipsy Lane, Oxford OX3 0BP, England..
    Stollewerk, Angelika
    Queen Mary Univ London, Sch Biol & Chem Sci, Mile End Rd, London E1 4NS, England..
    Torres-Oliva, Montserrat
    Univ Goettingen, Johann Friedrich Blumenbach Inst Zool & Anthropol, Dept Dev Biol, GZMB Ernst Caspari Haus, Justus von Liebig Weg 11, D-37077 Gottingen, Germany..
    Turetzek, Natascha
    Univ Goettingen, Johann Friedrich Blumenbach Inst Zool & Anthropol, Dept Dev Biol, GZMB Ernst Caspari Haus, Justus von Liebig Weg 11, D-37077 Gottingen, Germany..
    Vanthournout, Bram
    Aarhus Univ, Dept Biosci, Ny Munkegade 116,Bldg 1540, DK-8000 Aarhus C, Denmark.;Univ Ghent, Dept Biol, Evolut & Opt Nanostruct Grp EON, Ghent, Belgium..
    Werren, John H.
    Univ Rochester, Dept Biol, Rochester, NY 14627 USA..
    Wolff, Carsten
    Humboldt Univ, Inst Biol, Philippstr 13, D-10115 Berlin, Germany..
    Worley, Kim C.
    Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA..
    Bucher, Gregor
    Georg August Univ, GZMB, Johann Friedrich Blumenbach Inst, Dept Evolutionary Dev Genet, Gottingen Campus,Justus von Liebig Weg 11, D-37077 Gottingen, Germany..
    Gibbs, Richard A.
    Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA..
    Coddington, Jonathan
    Smithsonian Natl Museum Nat Hist, MRC 163,POB 37012, Washington, DC 20013 USA..
    Oda, Hiroki
    JT Biohist Res Hall, 1-1 Murasaki Cho, Takatsuki, Osaka 5691125, Japan.;Osaka Univ, Grad Sch Sci, Dept Biol Sci, Osaka, Japan..
    Stanke, Mario
    Ernst Moritz Arndt Univ Greifswald, Inst Math & Comp Sci, Walther Rathenau Str 47, D-17487 Greifswald, Germany..
    Ayoub, Nadia A.
    Washington & Lee Univ, Dept Biol, 204 West Washington St, Lexington, VA 24450 USA..
    Prpic, Nikola-Michael
    Univ Goettingen, Johann Friedrich Blumenbach Inst Zool & Anthropol, Dept Dev Biol, GZMB Ernst Caspari Haus, Justus von Liebig Weg 11, D-37077 Gottingen, Germany..
    Flot, Jean-Francois
    ULB, Evolutionary Biol & Ecol, CP 160-12,Ave FD Roosevelt 50, B-1050 Brussels, Belgium..
    Posnien, Nico
    Univ Goettingen, Johann Friedrich Blumenbach Inst Zool & Anthropol, Dept Dev Biol, GZMB Ernst Caspari Haus, Justus von Liebig Weg 11, D-37077 Gottingen, Germany..
    Richards, Stephen
    Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA..
    McGregor, Alistair P.
    Oxford Brookes Univ, Dept Biol & Med Sci, Gipsy Lane, Oxford OX3 0BP, England..
    The house spider genome reveals an ancient whole-genome duplication during arachnid evolution2017In: BMC Biology, ISSN 1741-7007, E-ISSN 1741-7007, Vol. 15, 62Article in journal (Refereed)
    Abstract [en]

    Background: The duplication of genes can occur through various mechanisms and is thought to make a major contribution to the evolutionary diversification of organisms. There is increasing evidence for a large-scale duplication of genes in some chelicerate lineages including two rounds of whole genome duplication (WGD) in horseshoe crabs. To investigate this further, we sequenced and analyzed the genome of the common house spider Parasteatoda tepidariorum.

    Results: We found pervasive duplication of both coding and non-coding genes in this spider, including two clusters of Hox genes. Analysis of synteny conservation across the P. tepidariorum genome suggests that there has been an ancient WGD in spiders. Comparison with the genomes of other chelicerates, including that of the newly sequenced bark scorpion Centruroides sculpturatus, suggests that this event occurred in the common ancestor of spiders and scorpions, and is probably independent of the WGDs in horseshoe crabs. Furthermore, characterization of the sequence and expression of the Hox paralogs in P. tepidariorum suggests that many have been subject to neo-functionalization and/or sub-functionalization since their duplication.

    Conclusions: Our results reveal that spiders and scorpions are likely the descendants of a polyploid ancestor that lived more than 450 MYA. Given the extensive morphological diversity and ecological adaptations found among these animals, rivaling those of vertebrates, our study of the ancient WGD event in Arachnopulmonata provides a new comparative platform to explore common and divergent evolutionary outcomes of polyploidization events across eukaryotes.

  • Liu, Wei
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Otolaryngology and Head and Neck Surgery.
    Schrott-Fischer, Annelies
    Med Univ Innsbruck, Dept Otolaryngol, Innsbruck, Austria..
    Glueckert, Rudolf
    Med Univ Innsbruck, Dept Otolaryngol, Innsbruck, Austria..
    Benav, Heval
    MED EL GmbH, R&D, Innsbruck, Austria..
    Rask-Andersen, Helge
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Otolaryngology and Head and Neck Surgery.
    The Human "Cochlear Battery" - Claudin-11 Barrier and Ion Transport Proteins in the Lateral Wall of the Cochlea2017In: Frontiers in Molecular Neuroscience, ISSN 1662-5099, Vol. 10, 239Article in journal (Refereed)
    Abstract [en]

    Background: The cochlea produces an electric field potential essential for hair cell transduction and hearing. This biological "battery" is situated in the lateral wall of the cochlea and contains molecular machinery that secretes and recycles K+ ions. Its functioning depends on junctional proteins that restrict the para-cellular escape of ions. The tight junction protein Claudin-11 has been found to be one of the major constituents of this barrier that maintains ion gradients (Gow et al., 2004; Kitajiri et al., 2004a). We are the first to elucidate the human Claudin-11 framework and the associated ion transport machinery using super-resolution fluorescence illumination microscopy (SR-SIM). Methods: Archival cochleae obtained during meningioma surgery were used for SR-SIM together with transmission electron microscopy after ethical consent. Results: Claudin-11-expressing cells formed parallel tight junction lamellae that insulated the epithelial syncytium of the stria vascularis and extended to the suprastrial region. Intercellular gap junctions were found between the barrier cells and fibrocytes. Conclusion: Transmission electron microscopy, confocal microscopy and SR-SIM revealed exclusive cell specialization in the various subdomains of the lateral wall of the human cochlea. The Claudin-11-expressing cells exhibited both conductor and isolator characteristics, and these micro-porous separators may selectively mediate the movement of charged units to the intrastrial space in a manner that is analogous to a conventional electrochemical "battery." The function and relevance of this battery for the development of inner ear disease are discussed.

  • Rossen, Jenny
    et al.
    Sophiahemmet Univ, Stockholm, Sweden.;Karolinska Inst, Sodersjukhuset, Dept Clin Sci & Educ, Stockholm, Sweden..
    Buman, Matthew P.
    Arizona State Univ, Sch Nutr & Hlth Promot, Coll Hlth Solut, Phoenix, AZ USA..
    Johansson, Unn-Britt
    Sophiahemmet Univ, Stockholm, Sweden.;Karolinska Inst, Sodersjukhuset, Dept Clin Sci & Educ, Stockholm, Sweden..
    Yngve, Agneta
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Social Sciences, Department of Food, Nutrition and Dietetics. Sophiahemmet Univ, Stockholm, Sweden.
    Ainsworth, Barbara
    Arizona State Univ, Sch Nutr & Hlth Promot, Coll Hlth Solut, Phoenix, AZ USA..
    Brismar, Kerstin
    Karolinska Inst, Dept Mol Med & Surg, Karolinska Univ Hosp, Rolf Luft Res Ctr Diabet & Endocrinol, Stockholm, Sweden..
    Hagströmer, Maria
    Karolinska Inst, Div Physiotherapy, Dept Neurobiol Care Sci & Soc, Stockholm, Sweden.;Karolinska Univ Hosp, Allied Hlth Profess Funct, Funct Area Occupat Therapy & Physiotherapy, Stockholm, Sweden..
    Reallocating bouted sedentary time to non-bouted sedentary time, light activity and moderate-vigorous physical activity in adults with prediabetes and type 2 diabetes2017In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 7, e0181053Article in journal (Refereed)
    Abstract [en]

    Aim: The aim of this study was to investigate the potential associations of reallocating 30 minutes sedentary time in long bouts (>60 min) to sedentary time in non-bouts, light intensity physical activity (LPA) and moderate-to vigorous physical activity (MVPA) with cardiometabolic risk factors in a population diagnosed with prediabetes or type 2 diabetes.

    Methods: Participants diagnosed with prediabetes and type 2 diabetes (n = 124, 50% men, mean [SD] age = 63.8 [7.5] years) were recruited to the physical activity intervention Sophia Step Study. For this study baseline data was used with a cross-sectional design. Time spent in sedentary behaviors in bouts (>60 min) and non-bouts (accrued in <60 min bouts) and physical activity was measured using the ActiGraph GT1M. Associations of reallocating bouted sedentary time to non-bouted sedentary time, LPA and MVPA with cardiometabolic risk factors were examined using an isotemporal substitution framework with linear regression models.

    Results: Reallocating 30 minutes sedentary time in bouts to MVPA was associated with lower waist circumference (b = -4.30 95% CI:-7.23, -1.38 cm), lower BMI (b = -1.46 95% CI:-2.60, -0.33 kg/m(2)) and higher HDL cholesterol levels (b = 0.11 95% CI: 0.02, 0.21 kg/m(2). Similar associations were seen for reallocation of sedentary time in non-bouts to MVPA. Reallocating sedentary time in bouts to LPA was associated only with lower waist circumference.

    Conclusion: Reallocation of sedentary time in bouts as well as non-bouts to MVPA, but not to LPA, was beneficially associated with waist circumference, BMI and HDL cholesterol in individuals with prediabetes and type 2 diabetes. The results of this study confirm the importance of reallocation sedentary time to MVPA.

  • Farag, Mohamed A.
    et al.
    Cairo Univ, Pharmacognosy Dept, Coll Pharm, Kasr el Aini St,PB 11562, Cairo, Egypt..
    Ali, Sara E.
    German Univ Cairo, Fac Pharm & Biotechnol, Dept Pharmaceut Biol, PB 11835, Cairo, Egypt..
    Hodaya, Rashad H.
    Desert Res Ctr, Plant Prod Dept, PB 11714, Cairo, Egypt..
    El-Seedi, Hesham R.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy. Menoufia Univ, Fac Sci, Dept Chem, Shibin Al Kawm 32512, Egypt..
    Sultani, Haider N.
    Leibniz Inst Plant Biochem, Dept Bioorgan Chem, Weinberg 3, D-06120 Halle, Saale, Germany..
    Laub, Annegret
    Leibniz Inst Plant Biochem, Dept Bioorgan Chem, Weinberg 3, D-06120 Halle, Saale, Germany..
    Eissa, Tarek E.
    Modern Sci & Arts Univ, Coll Pharm, Pharmacognosy Dept, PB 12566, Cairo, Egypt..
    Abou-Zaid, Fouad O. F.
    Desert Res Ctr, Plant Prod Dept, PB 11714, Cairo, Egypt..
    Wessjohann, Ludger A.
    Leibniz Inst Plant Biochem, Dept Bioorgan Chem, Weinberg 3, D-06120 Halle, Saale, Germany..
    Phytochemical Profiles and Antimicrobial Activities of Allium cepa Red cv. and A. sativum Subjected to Different Drying Methods: A Comparative MS-Based Metabolomics2017In: Molecules, ISSN 1420-3049, E-ISSN 1420-3049, Vol. 22, no 5, 761Article in journal (Refereed)
    Abstract [en]

    Plants of the Allium genus produce sulphur compounds that give them a characteristic (alliaceous) flavour and mediate for their medicinal use. In this study, the chemical composition and antimicrobial properties of Allium cepa red cv. and A. sativum in the context of three different drying processes were assessed using metabolomics. Bulbs were dried using either microwave, air drying, or freeze drying and further subjected to chemical analysis of their composition of volatile and non-volatile metabolites. Volatiles were collected using solid phase micro-extraction (SPME) coupled to gas chromatography-mass spectrometry (GC/MS) with 42 identified volatiles including 30 sulphur compounds, four nitriles, three aromatics, and three esters. Profiling of the polar non-volatile metabolites via ultra-performance liquid chromatography coupled to high resolution MS (UPLC/MS) annotated 51 metabolites including dipeptides, flavonoids, phenolic acids, and fatty acids. Major peaks in GC/MS or UPLC/MS contributing to the discrimination between A. sativum and A. cepa red cv. were assigned to sulphur compounds and flavonoids. Whereas sulphur conjugates amounted to the major forms in A. sativum, flavonoids predominated in the chemical composition of A. cepa red cv. With regard to drying impact on Allium metabolites, notable and clear separations among specimens were revealed using principal component analysis (PCA). The PCA scores plot of the UPLC/MS dataset showed closer metabolite composition of microwave dried specimens to freeze dried ones, and distant from air dried bulbs, observed in both A. cepa and A. sativum. Compared to GC/MS, the UPLC/MS derived PCA model was more consistent and better in assessing the impact of drying on Allium metabolism. A phthalate derivative was found exclusively in a commercial garlic preparation via GC/MS, of yet unknown origin. The freeze dried samples of both Allium species exhibited stronger antimicrobial activities compared to dried specimens with A. sativum being in general more active than A. cepa red cv.

  • Österberg, Erik
    Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology.
    Profiling memory accesses on the ODROID-XU42017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Decoupled Access-Execute(DAE) is an innovative approach to optimize energy consumption of computer programs by splitting the program into two tasks; the first task is to access data, this is profoundly memory-bound and can be done with energy efficient cores. The second task is to execute and compute the data, which iscompute-bound and can be done with powerful cores. This thesis work aims todevelop a profiling tool that can measure the efficiency of DAE by investigating thecache misses in the original code and the DAE code (in the access and executephases). This was achieved by measuring the cache loads and memory accesses forthe DAE transformation for the benchmarks done by a previous study that targets DAE on Arm's HMP architecture, big.LITTLE. The data obtained from this study showt hat DAE on big.LITTLE has a potential for energy savings, especially with applications that feature indirection in memory accesses. Arm DynamIQ opens up new possibilities for DAE code transformation. New levels of energy efficiency can be reached with a finer-grained Dynamic Voltage Frequency Scaling(DVFS), a more rapid power, state transition mechanism and a shared cache for 'big' and 'LITTLE' CPUs.

  • Aaboud, M.
    et al.
    Bergeås, Elin Kuutmann
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Brenner, Richard
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Ekelöf, Tord
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Ellert, Mattias
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Ferrari, Arnaud
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Gradin, P.O. Joakim
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Madsen, Alexander
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Öhman, Henrik
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Rangel-Smith, Camilla
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Zwalinski, L.
    Measurement of (WW +/-)-W-+/- vector-boson scattering and limits on anomalous quartic gauge couplings with the ATLAS detector2017In: Physical Review D: covering particles, fields, gravitation, and cosmology, ISSN 2470-0010, E-ISSN 2470-0029, Vol. 96, no 1, 012007Article in journal (Refereed)
    Abstract [en]

    This paper presents the extended results of measurements of (WW +/-)-W-+/- jj production and limits on anomalous quartic gauge couplings using 20.3 fb(-1) of proton-proton collision data at root s = 8 TeV recorded by the ATLAS detector at the Large Hadron Collider. Events with two leptons (e or mu) with the same electric charge and at least two jets are analyzed. Production cross sections are determined in two fiducial regions, with different sensitivities to the electroweak and strong production mechanisms. An additional fiducial region, particularly sensitive to anomalous quartic gauge coupling parameters alpha 4 and alpha 5, is introduced, which allows more stringent limits on these parameters compared to the previous ATLAS measurement.

  • Ramnerö, David
    Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Computerized Image Analysis and Human-Computer Interaction.
    Semi-automatic Training Data Generation for Cell Segmentation Network Using an Intermediary Curator Net2017Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    In this work we create an image analysis pipeline to segment cells from microscopy image data. A portion of the segmented images are manually curated and this curated data is used to train a Curator network to filter the whole dataset. The curated data is used to train a separate segmentation network to improve the cell segmentation. This technique can be easily applied to different types of microscopy object segmentation.

  • Petrus, P
    et al.
    Karolinska Inst, Karolinska Univ Hosp, Dept Med, Huddinge, Sweden.
    Edholm, David
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Upper Abdominal Surgery.
    Rosqvist, Fredrik
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Clinical Nutrition and Metabolism.
    Dahlman, I
    Karolinska Inst, Karolinska Univ Hosp, Dept Med, Huddinge, Sweden.
    Sundbom, Magnus
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Upper Abdominal Surgery.
    Arner, P
    Karolinska Inst, Karolinska Univ Hosp, Dept Med, Huddinge, Sweden.
    Rydén, M
    Karolinska Inst, Karolinska Univ Hosp, Dept Med, Huddinge, Sweden.
    Risérus, Ulf
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Clinical Nutrition and Metabolism.
    Depot-specific differences in fatty acid composition and distinct associations with lipogenic gene expression in abdominal adipose tissue of obese women2017In: International Journal of Obesity, ISSN 0307-0565, E-ISSN 1476-5497, Vol. 41, no 8, 1295-1298 p.Article in journal (Refereed)
    Abstract [en]

    Cardiometabolic diseases are primarily linked to enlarged visceral adipose tissue (VAT). However, some data suggest heterogeneity within the subcutaneous adipose tissue (SAT) depot with potential metabolic differences between the superficial SAT (sSAT) and deep SAT (dSAT) compartments. We aimed to investigate the heterogeneity of these three depots with regard to fatty acid (FA) composition and gene expression. Adipose tissue biopsies were collected from 75 obese women undergoing laparoscopic gastric bypass surgery. FA composition and gene expression were determined with gas chromatography and quantitative real-time-PCR, respectively. Stearoyl CoA desaturase-1 (SCD-1) activity was estimated by product-to-precursor FA ratios. All polyunsaturated FAs (PUFA) with 20 carbons were consistently lower in VAT than either SAT depots, whereas essential PUFA (linoleic acid, 18:2n-6 and α-linolenic acid, 18:3n-3) were similar between all three depots. Lauric and palmitic acid were higher and lower in VAT, respectively. The SCD-1 product palmitoleic acid as well as estimated SCD-1 activity was higher in VAT than SAT. Overall, there was a distinct association pattern between lipid metabolizing genes and individual FAs in VAT. In conclusion, SAT and VAT are two distinct depots with regard to FA composition and expression of key lipogenic genes. However, the small differences between sSAT and dSAT suggest that FA metabolism of SAT is rather homogenous.

  • Public defence: 2017-12-01 14:00 Enghoffsalen, Ing 50 bv, Akademiska sjukhuset, Uppsala
    Karlsson, Henning
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Cancer Pharmacology and Computational Medicine. Uppsala university.
    New preclinical strategies for characterization and development of anticancer drugs2017Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Increased understanding of the molecular mechanisms underlying cancer development has shifted drug discovery towards target driven drug development the last decades, but the development of effective cancer drugs has been hampered by the lack of predictive preclinical models. 3-D cultures, considered to more accurately reflect solid tumors in vivo, have been proposed as one way to increase the predictability of clinical efficacy in cancer drug discovery and development.

    The aims of this thesis were to improve preclinical models for cancer drug development, with focus on colorectal cancer (CRC) and use of multicellular tumor spheroids (MCTS), and also to mechanistically characterize some potentially new anticancer drugs (papers I – IV). The most important technical improvement was the development of direct measurement of green fluorescent protein (GFP) marked cells in spheroids, simplifying live collection of viability data and enabling high-throughput screening (HTS) in the MCTS model (paper I). In paper III and IV, the 3-D model was adapted to enable studies on the interaction between drugs and radiation. Two potentially new anticancer drugs, VLX50 and VLX60, were mechanistically characterized. VLX60, a novel copper containing thiosemicarbazone, induced reactive oxygen species (ROS) formation, was selectively active against BRAF mutated colon cancer cells and exhibited anticancer activity in vivo (paper II). Furthermore, two potentially new anticancer drugs were found suitable for further development for use in combination with radiation (papers III and IV). In paper III, synergy with radiation in spheroids compared to monolayer cultured colon cancer cells was shown with the novel iron-chelating inhibitor of oxidative phosphorylation, VLX600. In paper IV, the antiprotozoal drug nitazoxanide was shown to sensitize quiescent clonogenic colon cancer cells to radiation.

    In conclusion, introduction of measurement of fluorescence of GFP marked cells in spheroids makes clinically relevant 3-D models feasible for HTS experiments and characterization of candidate drugs and radiosensitizers in early cancer drug discovery and development. VLX60 has several characteristics suitable for further development into a cancer drug, notably against BRAF mutated colorectal cancer cells. VLX600 and nitazoxanide show radiosensitizing properties making them promising for further development for use as cancer drugs in combination with radiation.

    List of papers
    1. Loss of cancer drug activity in colon cancer HCT-116 cells during spheroid formation in a new 3-D spheroid cell culture system
    Open this publication in new window or tab >>Loss of cancer drug activity in colon cancer HCT-116 cells during spheroid formation in a new 3-D spheroid cell culture system
    2012 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 318, no 13, 1577-1585 p.Article in journal (Refereed) Published
    Abstract [en]

    Clinically relevant in vitro methods are needed to identify new cancer drugs for solid tumors. We report on a new 3-D spheroid cell culture system aimed to mimic the properties of solid tumors in vivo. The colon cancer cell lines HCT-116 wt and HCT-116 wt/GFP were grown as monolayers and for 3 or 6 days on 96-well NanoCulture (R) plates to form spheroids. Expression of surface markers, genes and hypoxia were assessed to characterize the spheroids and drug induced cytotoxicity was evaluated based on fluorescein diacetate (FDA) conversion by viable cells to fluorescent fluorescein or by direct measurement of fluorescence of GFP marked cells after a 72 h drug incubation. The cells reproducibly formed spheroids in the NanoCulture (R) plates with tight cell-attachment after 6 days. Cells in spheroids showed geno- and phenotypical properties reminiscent of hypoxic stem cells. Monolayer cultured cells were sensitive to standard and investigational drugs, whereas the spheroids gradually turned resistant. Similar results for cytotoxicity were observed using simplified direct measurement of fluorescence of GFP marked cells compared with FDA incubation. In conclusion, this new 3-D spheroid cell culture system provides a convenient and clinically relevant model for the identification and characterization of cancer drugs for solid tumors.

    Keyword
    Tumor cell, Cell culture, Monolayer, Spheroid, Cancer drug, Colon cancer
    National Category
    Cancer and Oncology
    Identifiers
    urn:nbn:se:uu:diva-177550 (URN)10.1016/j.yexcr.2012.03.026 (DOI)000305591800011 ()
    Available from: 2012-07-16 Created: 2012-07-16 Last updated: 2017-10-09Bibliographically approved
    2. Mechanistic characterization of a copper containing thiosemicarbazone with potent antitumor activity
    Open this publication in new window or tab >>Mechanistic characterization of a copper containing thiosemicarbazone with potent antitumor activity
    Show others...
    2017 (English)In: OncoTarget, ISSN 1949-2553, E-ISSN 1949-2553, Vol. 8, no 18, 30217-30234 p.Article in journal (Refereed) Published
    Abstract [en]

    Background: The thiosemicarbazone CD 02750 (VLX50) was recently reported as a hit compound in a phenotype-based drug screen in primary cultures of patient tumor cells. We synthesized a copper complex of VLX50, denoted VLX60, and characterized its antitumor and mechanistic properties.

    Materials and Methods: The cytotoxic effects and mechanistic properties of VLX60 were investigated in monolayer cultures of multiple human cell lines, in tumor cells from patients, in a 3-D spheroid cell culture system and in vivo and were compared with those of VLX50.

    Results: VLX60 showed >= 3-fold higher cytotoxic activity than VLX50 in 2-D cultures and, in contrast to VLX50, retained its activity in the presence of additional iron. VLX60 was effective against non-proliferative spheroids and against tumor xenografts in vivo in a murine model. In contrast to VLX50, gene expression analysis demonstrated that genes associated with oxidative stress were considerably enriched in cells exposed to VLX60 as was induction of reactive oxygen. VLX60 compromised the ubiquitin-proteasome system and was more active in BRAF mutated versus BRAF wild-type colon cancer cells.

    Conclusions: The cytotoxic effects of the copper thiosemicarbazone VLX60 differ from those of VLX50 and shows interesting features as a potential antitumor drug, notably against BRAF mutated colorectal cancer.

    Place, publisher, year, edition, pages
    IMPACT JOURNALS LLC, 2017
    Keyword
    cancer drug, thiosemicarbazone, spheroid, VLX60, BRAF
    National Category
    Cancer and Oncology
    Identifiers
    urn:nbn:se:uu:diva-323035 (URN)10.18632/oncotarget.16324 (DOI)000400456200055 ()28415818 (PubMedID)
    Funder
    Swedish Cancer SocietySwedish Foundation for Strategic Research
    Available from: 2017-06-01 Created: 2017-06-01 Last updated: 2017-10-09Bibliographically approved
    3. A novel tumor spheroid model identifies selective enhancement of radiation by an inhibitor of oxidative phosphorylation
    Open this publication in new window or tab >>A novel tumor spheroid model identifies selective enhancement of radiation by an inhibitor of oxidative phosphorylation
    Show others...
    (English)In: Article in journal (Other academic) Submitted
    Place, publisher, year, edition, pages
    Uppsala:
    National Category
    Cancer and Oncology
    Identifiers
    urn:nbn:se:uu:diva-330981 (URN)
    Available from: 2017-10-09 Created: 2017-10-09 Last updated: 2017-10-09
    4. Selective radiosensitization by nitazoxanide of quiescent clonogenic colon cancer tumor cells
    Open this publication in new window or tab >>Selective radiosensitization by nitazoxanide of quiescent clonogenic colon cancer tumor cells
    Show others...
    (English)Manuscript (preprint) (Other academic)
    National Category
    Cancer and Oncology
    Identifiers
    urn:nbn:se:uu:diva-330980 (URN)
    Available from: 2017-10-09 Created: 2017-10-09 Last updated: 2017-10-09
  • Blomqvist, Klara
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Malmberg, Isabell
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Att förlora ett bröst: En litteraturstudie om kvinnors psykiska hälsa efter mastektomi2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Background: Breast cancer is the most common form of cancer among women today, however, the survival rate has increased due to the development of more effective treatment options. Mastectomy has for a long time been the standard treatment but today, breast conserving surgery along with adjuvant therapy is considered to be an equally good treatment. It is therefore necessary to discuss the effect mastectomy has on mental health in light of these new treatment options. This literature review is important because it may enlighten nurses on this topic and provides conditions for correct handling and care of post-operative women.

     

    Aim: The purpose of this study is to investigate whether and how women’s mental health is affected by undergoing mastectomy as surgical treatment for breast cancer.

     

    Method: A general literature review. The searches were conducted in the databases PubMed, Cochrane, Cinahl and PsychINFO. A total 14 science articles with qualitative and quantitative design were included.  

     

    Results: Mental ill health, such as depression and anxiety, are common after undergoing a mastectomy and seems to be affected more negatively for those undergoing mastectomy than those undergoing breast conserving surgery and/or reconstruction. However, the mental ill health seem to decrease over time from before surgery to after. The body image is also affected negatively, more negatively after mastectomy compared to breast conserving surgery and/or reconstruction. The psychosocial health and quality of life appears to be lower than the general population.

     

    Conclusions: This literature review finds that mental ill health are common among women that have undergone a mastectomy. More research is required in order to understand how mental health changes over time among women undergoing mastectomy.

  • Jansson, Olivia
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Magnusson, Elin
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Vilka anledningar finns till att föräldrar väljer att inte vaccinera sina barn?: En litteraturöversikt2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Background: Vaccine protects both individuals and the society in general. Without vaccines, both herd-immunity and public health are threatened. Around the world, some parents choose not to vaccinate their children. This decision is often based on information the parents have received from internet sources. This study aimed to investigate the reasons why parents refrain from vaccinating their children. This information is important in the nurse's profession as the role involves supporting the patient in his or her self-care. Method: Through a systematic review, articles regarding vaccine resistance and hesitancy have been examined to answer the purpose of this study. A literature search was made in scientific databases, and 13 articles were selected. The articles were reviewed and various reasons for the vaccine resistance were found. Result: Things that parents worried about were side effects, illnesses and the content of the vaccine. Some parents did not believe that the disease exists anymore or thought that the vaccine is not reliable. Most of the parents' decisions aimed to protect their child and were, in most cases, made by lack of information. Conclusion: The conclusion of this review is that more reliable information must be available for parents. The information should come from reliable sources, such as authorities. The authorities should also reach out with information through the internet and social media, as parents tend to rely on the internet whilst making decisions. With information from this study nurses can help the parents make an informed decision regarding vaccinations. 

  • Dahl, Viktor
    et al.
    Publ Hlth Agcy Sweden, Stockholm, Sweden.;European Ctr Dis Prevent & Control ECDC, EPIET, Stockholm, Sweden..
    Wallensten, Anders
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. Publ Hlth Agcy Sweden, Stockholm, Sweden..
    Self-reported infections during international travel and notifiable infections among returning international travellers, Sweden, 2009-20132017In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 7, e0181625Article in journal (Refereed)
    Abstract [en]

    We studied food and water-borne diseases (FWDs), sexually transmitted diseases (STDs), vector-borne diseases (VBDs) and diseases vaccinated against in the Swedish childhood vaccination programme among Swedish international travellers, in order to identify countries associated with a high number of infections. We used the national database for notifiable infections to estimate the number of FWDs (campylobacteriosis, salmonellosis, giardiasis, shigellosis, EHEC, Entamoeba histolytica, yersinosis, hepatitis A, paratyphoid fever, typhoid fever, hepatitis E, listeriosis, cholera), STIs (chlamydia, gonorrhoea and acute hepatitis B), VBDs (dengue fever, malaria, West Nile fever, Japanese encephalitis and yellow fever) and diseases vaccinated against in the Swedish childhood vaccination programme (pertussis, measles, mumps, rubella, diphtheria) acquired abroad 2009-2013. We obtained number and duration of trips to each country from a database that monthly collects travel data from a randomly selected proportion of the Swedish population. We calculated number of infections per country 2009-2013 and incidence/million travel days for the five countries with the highest number of infections. Thailand had the highest number of FWDs (7,697, incidence 191/million travel days), STIs (1,388, incidence 34/million travel days) and VBDs (358, incidence 9/million travel days). France had the highest number of cases of diseases vaccinated against in the Swedish childhood vaccination programme (8, 0.4/million travel days). Swedish travellers contracted most infections in Thailand. Special focus should be placed on giving advice to travellers to this destination.

  • Philipson, Sara
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Widfeldt, Sigrid
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Faktorer som påverkar upplevelsen av bemötande hos personer med substansbrukssyndrom2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Introduction: People who use drugs are particularly vulnerable patients within health care, due to stigmatization and poor attitudes from healthcare professionals. Poor treatment can cause negative consequences for the patient’s overall health. Aim: To explore factors affecting how patients with substance use disorder experience treatment from healthcare professionals. Method: Literature review of qualitative studies. Data is collected from databases PubMed, PsycInfo, Scopus, ScienceDirect and CINAHL. Result: A person centered approach has a positive effect on the experience of treatment. Stigmatization and lack of formal competence are factors contributing to a negative experience of treatment from healthcare professionals. Conclusion: The factors contributing to the experience of treatment from healthcare professionals are if person centered care is applied, if stigmatizing behaviors and attitudes are present, and the formal nursing education levels related to substance use disorders. Individuals with substance use disorders may experience that treatment received by healthcare professionals affect their decision to seek care. Improvement and extension of nurse’s education related to substance use disorder and treatment within health care should be a priority.

  • Arnsäter, Olivia
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Janerheim, Johanna
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Faktorer i samarbetet och kommunikationen mellan sjuksköterskor och undersköterskor som påverkar en patientsäker omvårdnad: En intervjustudie2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Background: Patient safety is a constantly current subject. Collaboration and communication plays an important role for patient safety. Licensed practical nurses, registred nurses and physicians often works separately, without consulting each other. There are great risks with insufficient communication. The most commonly reported cause to adverse events are lack of communication within or between professions, units, shifts and caregivers.

    Aim: The aim of this study was to describe factors in the collaboration and communication between registred nurses and licensed practical nurses that can affect patient safe care on a hospital department.

    Method: The study was conducted using qualitative methods and based on half-structured interview questions with ten nurses, from two different departments and two different hospitals in Sweden. Interviews were recorded and transcribed as well as analyzed by qualitative content analysis.

    Result: The analyze of the responces gave four categories and six subcategories which was Control of care with subcategories Clarity and To have time, Common platform with subcategories Consensus and Reconciliation, Good group dynamics with subcategories Trust and Team spirit and Experience and competence.

    Conclusion: There are many factors in the collaboration and communication between registered nurses and licensed practical nurses that can affect a patient safe care. Factors as to have clarity, contribute to good group dynamics and to have the right competence the individual can impact. Other factors as environment, team training and work load are depending on decisions from higher instances.

  • Bermudez, Fanny
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Larsson, Frida
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Kvinnans livskvalitet och sexuella funktion efter hysterektomi: En litteraturstudie2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Background: Hysterectomy is a relatively common gynecological surgery that can be performed in three different ways: abdominally, vaginally or laparoscopically. It has been suggested that women with benign cause for a hysterectomy suffers from many symptoms that can lower a woman's overall quality of life and sexual function. Many women experience anxiety prior to the operation concerning the outcomes of the surgery.

     

    Aim: The purpose is to investigate how women estimate their quality of life and sexual function after an elective hysterectomy with a benign cause.

     

    Method: A literature review where 13 quantitative original articles were examined. The result was compiled and analyzed using a content analysis method.

     

    Results: Most of the articles reported that women's quality of life and sexual function improved after the hysterectomy. The women's social function, physical function, mental function and their pain problems had improved. This included that the women’s sexual frequency and sexual activity improved but their sexual desire was unchanged. In a contrary note some women estimated their FSFI higher, whilst in two other studies it was shown that some had FSFI which also concluded an impaired sexual function. Although women estimated their FSFI higher postoperatively their scores still show lower FSFI score than the normal population.

     

    Conclusion: Most women who go through with a hysterectomy because of benign diseases evaluate their quality of life to be higher postoperatively by improving their social function, physical function and mental health. Their sexual function is estimated to be higher than it was preoperatively, even though women still show lower FSFI score than the normal population.

  • Wang, Jinfan
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structure and Molecular Biology.
    Forster, Anthony C.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structure and Molecular Biology.
    Translational roles of the C75 2 ' OH in an in vitro tRNA transcript at the ribosomal A, P and E sites2017In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, 6709Article in journal (Refereed)
    Abstract [en]

    Aminoacyl-tRNAs containing a deoxy substitution in the penultimate nucleotide (C75 2'OH -> 2'H) have been widely used in translation for incorporation of unnatural amino acids (AAs). However, this supposedly innocuous modification surprisingly increased peptidyl-tRNA(ugc)(Ala) drop off in biochemical assays of successive incorporations. Here we predict the function of this tRNA 2'OH in the ribosomal A, P and E sites using recent co-crystal structures of ribosomes and tRNA substrates and test these structure-function models by systematic kinetics analyses. Unexpectedly, the C75 2'H did not affect A-to P-site translocation nor peptidyl donor activity of tRNA(ugc)(Ala). Rather, the peptidyl acceptor activity of the A-site Ala-tRNA(ugc)(Ala) and the translocation of the P-site deacylated tRNA(ugc)(Ala) to the E site were impeded. Delivery by EF-Tu was not significantly affected. This broadens our view of the roles of 2'OH groups in tRNAs in translation.

  • Matricon, Pierre
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Computational Biology and Bioinformatics. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Ranganathan, Anirudh
    Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, SE-10691 Stockholm, Sweden..
    Warnick, Eugene
    NIDDK, Mol Recognit Sect, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA..
    Gao, Zhan-Guo
    NIDDK, Mol Recognit Sect, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA..
    Rudling, Axel
    Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, SE-10691 Stockholm, Sweden..
    Lambertucci, Catia
    Univ Camerino, Scuola Sci Farmaco & Prod Salute, Via S Agostino 1, I-62032 Camerino, MC, Italy..
    Marucci, Gabriella
    Univ Camerino, Scuola Sci Farmaco & Prod Salute, Via S Agostino 1, I-62032 Camerino, MC, Italy..
    Ezzati, Aitakin
    Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, SE-10691 Stockholm, Sweden..
    Jaiteh, Mariama
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Computational Biology and Bioinformatics. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Dal Ben, Diego
    Univ Camerino, Scuola Sci Farmaco & Prod Salute, Via S Agostino 1, I-62032 Camerino, MC, Italy..
    Jacobson, Kenneth A.
    NIDDK, Mol Recognit Sect, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA..
    Carlsson, Jens
    Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Computational Biology and Bioinformatics.
    Fragment optimization for GPCRs by molecular dynamics free energy calculations: Probing druggable subpockets of the A(2A) adenosine receptor binding site2017In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, 6398Article in journal (Refereed)
    Abstract [en]

    Fragment-based lead discovery is becoming an increasingly popular strategy for drug discovery. Fragment screening identifies weakly binding compounds that require optimization to become high-affinity leads. As design of leads from fragments is challenging, reliable computational methods to guide optimization would be invaluable. We evaluated using molecular dynamics simulations and the free energy perturbation method (MD/FEP) in fragment optimization for the A(2A) adenosine receptor, a pharmaceutically relevant G protein-coupled receptor. Optimization of fragments exploring two binding site subpockets was probed by calculating relative binding affinities for 23 adenine derivatives, resulting in strong agreement with experimental data (R-2 = 0.78). The predictive power of MD/FEP was significantly better than that of an empirical scoring function. We also demonstrated the potential of the MD/FEP to assess multiple binding modes and to tailor the thermodynamic profile of ligands during optimization. Finally, MD/FEP was applied prospectively to optimize three nonpurine fragments, and predictions for 12 compounds were evaluated experimentally. The direction of the change in binding affinity was correctly predicted in a majority of the cases, and agreement with experiment could be improved with rigorous parameter derivation. The results suggest that MD/FEP will become a powerful tool in structure-driven optimization of fragments to lead candidates.

  • Bowman, John L
    et al.
    Kohchi, Takayuki
    Yamato, Katsuyuki T
    Jenkins, Jerry
    Shu, Shengqiang
    Ishizaki, Kimitsune
    Yamaoka, Shohei
    Nishihama, Ryuichi
    Nakamura, Yasukazu
    Berger, Frédéric
    Adam, Catherine
    Aki, Shiori Sugamata
    Althoff, Felix
    Araki, Takashi
    Arteaga-Vazquez, Mario A
    Balasubrmanian, Sureshkumar
    Barry, Kerrie
    Bauer, Diane
    Boehm, Christian R
    Briginshaw, Liam
    Caballero-Perez, Juan
    Catarino, Bruno
    Chen, Feng
    Chiyoda, Shota
    Chovatia, Mansi
    Davies, Kevin M
    Delmans, Mihails
    Demura, Taku
    Dierschke, Tom
    Dolan, Liam
    Dorantes-Acosta, Ana E
    Eklund, D. Magnus
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Plant Ecology and Evolution.
    Florent, Stevie N
    Flores-Sandoval, Eduardo
    Fujiyama, Asao
    Fukuzawa, Hideya
    Galik, Bence
    Grimanelli, Daniel
    Grimwood, Jane
    Grossniklaus, Ueli
    Hamada, Takahiro
    Haseloff, Jim
    Hetherington, Alexander J
    Higo, Asuka
    Hirakawa, Yuki
    Hundley, Hope N
    Ikeda, Yoko
    Inoue, Keisuke
    Inoue, Shin-Ichiro
    Ishida, Sakiko
    Jia, Qidong
    Kakita, Mitsuru
    Kanazawa, Takehiko
    Kawai, Yosuke
    Kawashima, Tomokazu
    Kennedy, Megan
    Kinose, Keita
    Kinoshita, Toshinori
    Kohara, Yuji
    Koide, Eri
    Komatsu, Kenji
    Kopischke, Sarah
    Kubo, Minoru
    Kyozuka, Junko
    Lagercrantz, Ulf
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Plant Ecology and Evolution.
    Lin, Shih-Shun
    Lindquist, Erika
    Lipzen, Anna M
    Lu, Chia-Wei
    De Luna, Efraín
    Martienssen, Robert A
    Minamino, Naoki
    Mizutani, Masaharu
    Mizutani, Miya
    Mochizuki, Nobuyoshi
    Monte, Isabel
    Mosher, Rebecca
    Nagasaki, Hideki
    Nakagami, Hirofumi
    Naramoto, Satoshi
    Nishitani, Kazuhiko
    Ohtani, Misato
    Okamoto, Takashi
    Okumura, Masaki
    Phillips, Jeremy
    Pollak, Bernardo
    Reinders, Anke
    Rövekamp, Moritz
    Sano, Ryosuke
    Sawa, Shinichiro
    Schmid, Marc W
    Shirakawa, Makoto
    Solano, Roberto
    Spunde, Alexander
    Suetsugu, Noriyuki
    Sugano, Sumio
    Sugiyama, Akifumi
    Sun, Rui
    Suzuki, Yutaka
    Takenaka, Mizuki
    Takezawa, Daisuke
    Tomogane, Hirokazu
    Tsuzuki, Masayuki
    Ueda, Takashi
    Umeda, Masaaki
    Ward, John M
    Watanabe, Yuichiro
    Yazaki, Kazufumi
    Yokoyama, Ryusuke
    Yoshitake, Yoshihiro
    Yotsui, Izumi
    Zachgo, Sabine
    Schmutz, Jeremy
    Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome2017In: Cell, ISSN 0092-8674, E-ISSN 1097-4172, Vol. 171, no 2, 287-304 p.Article in journal (Refereed)
    Abstract [en]

    The evolution of land flora transformed the terrestrial environment. Land plants evolved from an ancestral charophycean alga from which they inherited developmental, biochemical, and cell biological attributes. Additional biochemical and physiological adaptations to land, and a life cycle with an alternation between multicellular haploid and diploid generations that facilitated efficient dispersal of desiccation tolerant spores, evolved in the ancestral land plant. We analyzed the genome of the liverwort Marchantia polymorpha, a member of a basal land plant lineage. Relative to charophycean algae, land plant genomes are characterized by genes encoding novel biochemical pathways, new phytohormone signaling pathways (notably auxin), expanded repertoires of signaling pathways, and increased diversity in some transcription factor families. Compared with other sequenced land plants, M. polymorpha exhibits low genetic redundancy in most regulatory pathways, with this portion of its genome resembling that predicted for the ancestral land plant. PAPERCLIP.

  • Public defence: 2017-12-02 09:30 Hedstrandsalen, Uppsala
    Schiza, Aglaia
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Experimental and Clinical Oncology.
    Experimental treatment of patients with disseminated malignant melanoma2017Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Malignant melanoma (MM) is the deadliest skin cancer with an ever-increasing incidence. New treatments have improved the prognosis for patients with advanced MM. Still, most patients do not respond, and the side effects can be severe, underlining the need for better therapies.

    The overall aim of this thesis was to evaluate new means to improve the treatment for patients with advanced MM. Immunostimulatory gene therapy (AdCD40L) was evaluated in a clinical study and BRAF-inhibitory treatment in rare cases of BRAF-mutated MM.

    Due to its immunogenicity, MM is an attractive target for immunostimulatory gene therapy. AdCD40L is an adenovirus carrying the human gene for CD40 ligand, which in different ways can stimulate the immune system to combat cancer. We conducted a Phase I/IIa study with AdCD40L in patients with metastatic MM having received established treatments. In cohort 1 (n=6), four weekly, intratumoural AdCD40L injections were given. In cohort 2 (n=9), low dose cyclophosphamide was added to increase the immune response. Since irradiation may act synergistically with immunotherapy, patients in cohort 3 (n=9) also received a single fraction of radiotherapy (8 Gy). This fraction was given towards the lesion selected for injections.

    The primary objectives were to assess the feasibility and safety of AdCD40L-treatment and secondarily its anti-tumour effects. Patients were thoroughly assessed for toxicity. The anti-tumour response was evaluated by imaging techniques (FDG-PET/CT, DW-MRI scans), tumour biopsies and blood tests. Plasma protein markers were measured with a multiplex platform. Another objective was to evaluate the potential of DW-MRI and FDG-PET/CT for prediction of AdCD40L treatment response, in terms of overall survival (OS).

    AdCD40L was well tolerated with mild transient reactions. Local and distant responses in PET/CT scans along with a significantly better 6-month survival in the cohorts that received cyclophosphamide conditioning were observed. Effector lymphocyte responses were elicited. All patients had an increased T effector/T regulatory-cell ratio and death receptors were significantly up-regulated post therapy. Inflammatory cytokines and other plasma proteins were altered in favourable ways by the AdCD40L treatment. The analyses support that the functional DWI parameters may be better early predictors of OS than the established metabolic and morphologic criteria of FDG-PET/CT and CT/MRI, respectively.

    In conclusion, the stimulation of the CD40 pathway to initiate anti-tumour immunity is a promising treatment alternative for MM patients. However, further studies with developed treatment schemes are warranted.

    In the first report ever on treatment of a pregnant patient with a BRAF-inhibitor, the therapy was initiated in the second trimester. The treatment with vemurafenib enabled prolonged gestation, hence reducing the risk of immaturity-related complications. Further, we report the first case worldwide of a patient with metastatic conjunctival melanoma who benefitted from treatment with vemurafenib. Additional studies are needed to assess the efficacy of BRAF -inhibitors in the different subtypes of ocular melanoma.

     

    List of papers
    1. Immunostimulatory AdCD40L gene therapy combined with low-dose cyclophosphamide in metastatic melanoma patients
    Open this publication in new window or tab >>Immunostimulatory AdCD40L gene therapy combined with low-dose cyclophosphamide in metastatic melanoma patients
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    2016 (English)In: British Journal of Cancer, ISSN 0007-0920, E-ISSN 1532-1827, Vol. 114, no 8, 872-880 p.Article in journal (Refereed) Published
    Abstract [en]

    BACKGROUND: Current approaches for treating metastatic malignant melanoma (MM) are not effective enough and are associated with serious adverse events. Due to its immunogenicity, melanoma is an attractive target for immunostimulating therapy. In this phase I/IIa study, local AdCD40L immunostimulatory gene therapy was evaluated in patients with MM.

    METHODS: AdCD40L is an adenovirus carrying the gene for CD40 ligand. Patients that failed standard treatments were enrolled. Six patients received four weekly intratumoral AdCD40L injections. Next, nine patients received low-dose cyclophosphamide conditioning before the first and fourth AdCD40L injection. The blood samples were collected at multiple time points for chemistry, haematology and immunology evaluations. Radiology was performed at enrolment and repeated twice after the treatment.

    RESULTS: AdCD40L was safe with mild transient reactions. No objective responses were recorded by MRI, however, local and distant responses were seen on FDG-PET. The overall survival at 6 months was significantly better when cyclophosphamide was added to AdCD40L. The patients with the best survival developed the highest levels of activated T cells and experienced a pronounced decrease of intratumoral IL8.

    CONCLUSIONS: AdCD40L therapy for MM was well tolerated. Local and distant responses along with better survival in the low-dose cyclophosphamide group are encouraging.

    National Category
    Cancer and Oncology
    Identifiers
    urn:nbn:se:uu:diva-295735 (URN)10.1038/bjc.2016.42 (DOI)000374129200004 ()27031851 (PubMedID)
    Funder
    Swedish Cancer Society
    Available from: 2016-06-09 Created: 2016-06-09 Last updated: 2017-10-15Bibliographically approved
    2. Adenovirus-mediated CD40L gene transfer increases Teffector/Tregulatory cell ratio and upregulates death receptors in metastatic melanoma patients
    Open this publication in new window or tab >>Adenovirus-mediated CD40L gene transfer increases Teffector/Tregulatory cell ratio and upregulates death receptors in metastatic melanoma patients
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    2017 (English)In: Journal of Translational Medicine, ISSN 1479-5876, E-ISSN 1479-5876, Vol. 15, no 79Article in journal (Refereed) Published
    Abstract [en]

    Background and aims: Malignant melanoma is an aggressive tumor sensitive for immunotherapy such as checkpoint blockade antibodies. Still, most patients with late stage disease do not respond, and the side effects can be severe. Stimulation of the CD40 pathway to initiate anti-tumor immunity is a promising alternative. Herein, we demonstrate immune profiling data from melanoma patients treated with an adenovirus-based CD40 ligand gene therapy (AdCD40L). Methods: Peripheral blood mononuclear cells and plasma were collected from malignant melanoma patients (n = 15) enrolled in a phase I/IIa study investigating intratumoral delivery of AdCD40L with or without low dose cyclophosphamide. Cells were analyzed by flow cytometry while plasma samples were analyzed by a multi-array proteomics. Results: All patients had an increased Teffector/Tregulatory cell ratio post therapy. Simultaneously, the death receptors TNFR1 and TRAIL-R2 were significantly up-regulated post treatment. Stem cell factor (SCF), E-selectin, and CD6 correlated to enhanced overall survival while a high level of granulocytic myeloid-derived suppressor cells (gMDSCs), IL8, IL10, TGFb1, CCL4, PlGF and Fl3t ligand was highest in patients with short survival. Conclusions: AdCD40L intratumoral injection induced desirable systemic immune effects that correlated to prolonged survival. Further studies using CD40 stimulation in malignant melanoma are warranted.

    Keyword
    AdCD40L, Malignant melanoma, Immunotherapy, Proteomics, T regulatory cells, Myeloid-derived suppressor cells
    National Category
    Immunology in the medical area
    Identifiers
    urn:nbn:se:uu:diva-322800 (URN)10.1186/s12967-017-1182-z (DOI)000399786900002 ()28427434 (PubMedID)
    Available from: 2017-06-20 Created: 2017-06-20 Last updated: 2017-10-15Bibliographically approved
    3. Diffusion-Weighted MRI may be better than FDG-PET/CT to assess immunotherapy response in patients with metastatic melanoma
    Open this publication in new window or tab >>Diffusion-Weighted MRI may be better than FDG-PET/CT to assess immunotherapy response in patients with metastatic melanoma
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    (English)Manuscript (preprint) (Other academic)
    National Category
    Cancer and Oncology
    Identifiers
    urn:nbn:se:uu:diva-330709 (URN)
    Available from: 2017-10-03 Created: 2017-10-03 Last updated: 2017-10-15
    4. Treatment of metastatic malignant melanoma with vemurafenib during pregnancy
    Open this publication in new window or tab >>Treatment of metastatic malignant melanoma with vemurafenib during pregnancy
    Show others...
    2013 (English)In: Journal of Clinical Oncology, ISSN 0732-183X, Vol. 31, no 11, e192-e193 p.Article in journal (Refereed) Published
    National Category
    Medical and Health Sciences
    Identifiers
    urn:nbn:se:uu:diva-328673 (URN)10.1200/JCO.2012.45.2870 (DOI)23401457 (PubMedID)
    Available from: 2017-08-29 Created: 2017-08-29 Last updated: 2017-10-15Bibliographically approved
    5. A case report of a patient with metastatic ocular melanoma who experienced a response to treatment with the BRAF inhibitor vemurafenib
    Open this publication in new window or tab >>A case report of a patient with metastatic ocular melanoma who experienced a response to treatment with the BRAF inhibitor vemurafenib
    2016 (English)In: BMC Cancer, ISSN 1471-2407, E-ISSN 1471-2407, Vol. 16, 634Article in journal (Refereed) Published
    Abstract [en]

    Background: Conjunctival malignant melanoma (CMM) is a rare malignancy and in the advanced setting there is no effective treatment. In contrast, half of cutaneous melanomas have BRAF mutations and treatment with BRAF inhibitors is established for patients with disseminated disease. The most common form of ocular melanoma, uveal melanoma, lacks these mutations, however, their presence has been reported for CMM. Case presentation: We used the BRAF inhibitor vemurafenib to treat a 53 year-old female suffering from a BRAFV600E mutated metastatic CMM. The patient benefited from the treatment, a response was evident within a week and she experienced a progression free survival of four months. Conclusions: To our knowledge, this is the first described case of response to vemurafenib treatment in a patient with ocular melanoma.

    Keyword
    BRAF inhibitor, BRAF mutation, Conjunctival malignant melanoma, Ocular melanoma, Vemurafenib
    National Category
    Cancer and Oncology
    Identifiers
    urn:nbn:se:uu:diva-305937 (URN)10.1186/s12885-016-2657-7 (DOI)000384169800001 ()27520988 (PubMedID)
    Available from: 2016-11-11 Created: 2016-10-24 Last updated: 2017-10-15Bibliographically approved
  • Eriksson, Leif
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health, International Maternal and Child Health (IMCH). Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Caring Sciences.
    Bergström, Anna
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health, International Maternal and Child Health (IMCH). Institute for Global Health, London, United Kingdom.
    Hoa, Dinh Thi Phuong
    Hanoi School of Public Health, Hanoi, Vietnam.
    Nga, Nguyen Thu
    Research Institute for Child Health, Hanoi, Vietnam.
    Eldh, Ann Catrine
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Health Services Research. Högskolan Dalarna.
    Sustainability of knowledge implementation in a low- and middle- income context: Experiences from a facilitation project in Vietnam targeting maternal and neonatal health2017In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 8, e0182626Article in journal (Refereed)
    Abstract [en]

    BACKGROUND: In a previous trial in Vietnam, a facilitation strategy to secure evidence-based practice in primary care resulted in reduced neonatal mortality over a period of three years. While little is known as to what ensures sustainability in the implementation of community-based strategies, the aim of this study was to investigate factors promoting or hindering implementation, and sustainability of knowledge implementation strategies, by means of the former Neonatal Knowledge Into Practice (NeoKIP) trial.

    METHODS: In 2014 we targeted all levels in the Vietnamese healthcare system: six individual interviews with representatives at national, provincial and district levels, and six focus group discussions with representatives at the commune level. The interviews were transcribed verbatim, translated to English, and analysed using inductive and deductive thematic analysis.

    RESULTS: To achieve successful implementation and sustained effect of community-based knowledge implementation strategies, engagement of leaders and key stakeholders at all levels of the healthcare system is vital-prior to, during and after a project. Implementation and sustainability require thorough needs assessment, tailoring of the intervention, and consideration of how to attain and manage funds. The NeoKIP trial was characterised by a high degree of engagement at the primary healthcare system level. Further, three years post trial, maternal and neonatal care was still high on the agenda for healthcare workers and leaders, even though primary aspects such as stakeholder engagement at all levels, and funding had been incomplete or lacking.

    CONCLUSIONS: The current study illustrates factors to support successful implementation and sustain effects of community-based strategies in projects in low- and middle-income settings; some but not all factors were represented during the post-NeoKIP era. Most importantly, trials in this and similar contexts require deliberate management throughout and beyond the project lifetime, and engagement of key stakeholders, in order to promote and sustain knowledge implementation.

  • Grandahl, Maria
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Caring Sciences.
    Larsson, Margareta
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health.
    Dalianis, Tina
    Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.
    Stenhammar, Christina
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health.
    Tydén, Tanja
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health.
    Westerling, Ragnar
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Social Medicine.
    Nevéus, Tryggve
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health, Research group (Dept. of women´s and children´s health), Paediatric Inflammation Research.
    Catch-up HPV vaccination status of adolescents in relation to socioeconomic factors, individual beliefs and sexual behaviour2017In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 11, e0187193Article in journal (Refereed)
    Abstract [en]

    In 2012, human papillomavirus (HPV) vaccination was introduced free of charge in the Swedish national school-based vaccination programme for 10-12-year-old girls, and as catch-up vaccination for young women. In Sweden, there is an ongoing discussion about including boys in the national vaccination programme. Few studies are undertaken about adolescents' knowledge, beliefs and HPV vaccination status in relation to socioeconomic status and sexual experience. Thus, the aim was to examine HPV catch-up vaccination status in adolescents in relation to 1) socioeconomic factors, 2) beliefs and knowledge about HPV prevention, and 3) sexual behaviour. The Health Belief Model was used as a theoretical framework. Upper secondary school students (n = 832) aged 16, randomly chosen from a larger sample, were invited to participate in conjunction with the general health interview with the school nurse. A total of 751/832 (90.3%), girls (n = 391, 52%) and boys (n = 360, 48%) completed the questionnaire. HPV vaccination was associated with ethnicity and the mothers' education level; i.e. girls with a non-European background and girls with a less educated mother were less likely to have received the vaccine (p<0.01 and p = 0.04 respectively). Vaccinated girls perceived HPV infection as more severe (p = 0.01), had more insight into women's susceptibility to the infection (p = 0.02), perceived more benefits of the vaccine as protection against cervical cancer (p<0.01) and had a higher intention to engage in HPV-preventive behaviour (p = 0.01). Furthermore, boys and girls were almost equally sexually experienced, although fewer girls had used condom during first intercourse with their latest partner (p = 0.03). Finally, HPV vaccinated girls were less likely to have unprotected sex (p<0.01). In summary, catch-up HPV vaccination among young girls was associated with a European background and high maternal education level, as well as more favourable beliefs towards HPV prevention and less sexual risk-taking. Further preventive measures should therefore be directed at the migrant population.

  • Weidner, Tim
    Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology.
    Investigating the Scalability of Direct-to-Master Caches2017Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    Upcoming processors will utilize an ever increasing number of transistors bye mploying them as multiple cores. With the growth in number of cores, current cache hierarchies become one of the limiting factors for the scalability of applications utilizing multi-core processors. D2M, a new split cache hierarchy design, provides a unified mechanism for cache searching, eviction, and coherence,that eliminates level-by-level data movement and searches. This work contributesto research on D2M by performing a scalability analysis using High-Performance Computing benchmarks from the SPLASH-2x benchmark suite. By conducting experiments for 2, 4 and 8 cores with the Gem5 full-system simulator, we provide ageneral scalability trend for D2M. The experiments specifically target scalability ofthe metadata hierarchy. MD3 lock granularity and shared region overhead are examined, resulting in a definite number of locks to avoid MD3 lock aliasing for the selected benchmarks. In addition, a detailed performance comparison between D2M and a generic standard cache hierarchy model is given for all core configurations.

  • Nilsson, Anna
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Peric, Alexandra
    AstraZeneca Gothenburg, Cardiovasc & Metab Dis, Innovat Med & Early Dev, Gothenburg, Sweden..
    Strimfors, Marie
    AstraZeneca Gothenburg, Cardiovasc & Metab Dis, Innovat Med & Early Dev, Gothenburg, Sweden..
    Goodwin, Richard J. A.
    AstraZeneca Cambridge, Mass Spectrometry Imaging, Innovat Med & Early Dev, Drug Safety & Metab, Cambridge, England..
    Hayes, Martin A.
    AstraZeneca Gothenburg, Cardiovasc & Metab Dis, Innovat Med & Early Dev, Gothenburg, Sweden..
    Andrén, Per E.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Hilgendorf, Constanze
    AstraZeneca Gothenburg, Cardiovasc & Metab Dis, Innovat Med & Early Dev, Gothenburg, Sweden.;AstraZeneca Gothenburg, Innovat Med & Early Dev, Drug Safety & Metab, Safety & ADME Translat Sci, Gothenburg, Sweden..
    Mass Spectrometry Imaging proves differential absorption profiles of well-characterised permeability markers along the crypt-villus axis2017In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, 6352Article in journal (Refereed)
    Abstract [en]

    Knowledge about the region-specific absorption profiles from the gastrointestinal tract of orally administered drugs is a critical factor guiding dosage form selection in drug development. We have used a novel approach to study three well-characterized permeability and absorption marker drugs in the intestine. Propranolol and metoprolol (highly permeable compounds) and atenolol (low-moderate permeability compound) were orally co-administered to rats. The site of drug absorption was revealed by high spatial resolution matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) and complemented by quantitative measurement of drug concentration in tissue homogenates. MALDI-MSI identified endogenous molecular markers that illustrated the villi structures and confirmed the different absorption sites assigned to histological landmarks for the three drugs. Propranolol and metoprolol showed a rapid absorption and shorter transit distance in contrast to atenolol, which was absorbed more slowly from more distal sites. This study provides novel insights into site specific absorption for each of the compounds along the crypt-villus axis, as well as confirming a proximal-distal absorption gradient along the intestine. The combined analytical approach allowed the quantification and spatial resolution of drug distribution in the intestine and provided experimental evidence for the suggested absorption behaviour of low and highly permeable compounds.

  • Melander, Erik
    Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology.
    Study of Bandwidth Partitioning for Co-executing GPU Kernels2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Co-executing GPU kernels on a partitioned GPU has been shown to improve utilization efficiency of poorly scaling tasks. While kernels can be executed in parallel, data transfers to the GPU are serial which can negatively impact parallelism and predictability of the kernels.In this work we implement a fairness-based approach to memory transfers by chunking data sets and transferring them interleaved and evaluate the overhead of this approach. Then we develop a model to predict when kernels will start using this implementation. We found that chunked transfers in a single CUDA stream have onlya small overhead compared to serial transfers, while event synchronized transfers in several streams have larger overhead particularly for chunk sizes less than 500 KB.The prediction models accurately estimate kernel starting times and return transfertimes with less than 2.7% relative error.

  • Lövgren, Simon
    Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology.
    Simulating Energy-Efficient Hardware The Software Out-of-order Processor2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    The modern trends for technology scaling are not extremely bright. The cost of transistors have leveled off recently, effectively halting the ability to put additional transistors on a chip for the same price. In addition, Dennard Scaling, what has allowed for switching additional transistors whilst scaling to smaller nodes isslowing significantly. This thesis, with focus on the hardware, proposes anenhanced stall-on-use in-order core hardware/software co-design which improves performance and energy efficiency by allowing out-of-program-order executionthrough allowing the hardware and software to communicate with one another --allowing the hardware to make dynamic decisions on how to direct execution flowto expose additional memory- and instruction level parallelism.The results are very promising where we see an increase in both performance (upto 3.7x speedup) and energy efficiency (up to 59% increase). While additional worki is needed to evaluate the extent of the benefits across a wide range of applications, SWOOP looks to be a good option for energy efficiency without compromisingperformance for memory-bound applications with MLP.

  • Lejdung, Johan
    Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology.
    System and database design for a research application to diagnostic laser-doppler instruments2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Perimed AB is interested in developing a new generation research platform, for thei rmost recent medical instrument, PF 6000. With thedevelopment of this platform, they are looking for innovative and practical ways to meet the requirements from the medical research community. Other than a versatile and easy-to-use system, Perimed has noticed a particular needto store and process the large amounts of data generatedby their instruments. With the use of a database, the platform can provide a searchable, trackable and versatile system. The database assures that all information is not only easily attained, but also shareable through various connections. After testinga total of four different databases against the requirementsof the system, a database was chosen to be implemented with the platform. A system design was detailed with the use of six different UML diagram-types. The design promoted a modular system and focused heavily on the data flow, from and to the instrument and database. The system design and the chosen database was then used to implement a prototype. Through the prototype, the database and system design were proven as a feasible solution for how to design and develop the system. Together with the diagrams, the prototype will serve as an excellent source of documentationfor Perimed during the development of the platform.

  • Yeo, Astrid
    et al.
    GlaxoSmithKline Med Res Ctr, Dept Genet, Stevenage, Herts, England..
    Li, Li
    GlaxoSmithKline Med Res Ctr, Dept Genet, Res Triangle Pk, NC USA.;PAREXEL Int, Genom Med, Durham, NC USA..
    Warren, Liling
    GlaxoSmithKline Med Res Ctr, Dept Genet, Res Triangle Pk, NC USA.;Teva Pharmaceut, Raleigh, NC USA..
    Aponte, Jennifer
    GlaxoSmithKline Med Res Ctr, Dept Genet, Res Triangle Pk, NC USA.;PAREXEL Int, Genom Med, Durham, NC USA..
    Fraser, Dana
    GlaxoSmithKline Med Res Ctr, Dept Genet, Res Triangle Pk, NC USA.;PAREXEL Int, Genom Med, Durham, NC USA..
    King, Karen
    GlaxoSmithKline Med Res Ctr, Dept Genet, Res Triangle Pk, NC USA.;PAREXEL Int, Genom Med, Durham, NC USA..
    Johansson, Kelley
    GlaxoSmithKline Med Res Ctr, Dept Genet, Res Triangle Pk, NC USA.;PAREXEL Int, Genom Med, Durham, NC USA..
    Barnes, Allison
    GlaxoSmithKline Med Res Ctr, Clin Stat, Res Triangle Pk, NC USA.;PAREXEL Int, Biostat, Durham, NC USA..
    MacPhee, Colin
    GlaxoSmithKline Med Res Ctr, Dept Vasc Biol & Thrombosis, King Of Prussia, PA USA..
    Davies, Richard
    GlaxoSmithKline Med Res Ctr, Metab Pathways & Cardiovasc Therapeut Area, King Of Prussia, PA USA..
    Chissoe, Stephanie
    GlaxoSmithKline Med Res Ctr, Dept Genet, Res Triangle Pk, NC USA.;GlaxoSmithKline, Elliott Ave, Seattle, WA USA..
    Tarka, Elizabeth
    GlaxoSmithKline Med Res Ctr, Metab Pathways & Cardiovasc Therapeut Area, King Of Prussia, PA USA.;Janssen Pharmaceut, Spring House, PA USA..
    O'Donoghue, Michelle L.
    Brigham & Womens Hosp, Div Cardiovasc, TIMI Study Grp, 75 Francis St, Boston, MA 02115 USA..
    White, Harvey D.
    Auckland City Hosp, Green Lane Cardiovasc Serv, Auckland, New Zealand.;Univ Auckland, Auckland, New Zealand..
    Wallentin, Lars
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Cardiology. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, UCR-Uppsala Clinical Research Center.
    Waterworth, Dawn
    GlaxoSmithKline Med Res Ctr, Dept Genet, Philadelphia, PA USA..
    Pharmacogenetic meta-analysis of baseline risk factors, pharmacodynamic, efficacy and tolerability endpoints from two large global cardiovascular outcomes trials for darapladib2017In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 7, e0182115Article in journal (Refereed)
    Abstract [en]

    Darapladib, a lipoprotein-associated phospholipase A2 (Lp-PLA(2)) inhibitor, failed to demonstrate efficacy for the primary endpoints in two large phase III cardiovascular outcomes trials, one in stable coronary heart disease patients (STABILITY) and one in acute coronary syndrome (SOLID-TIMI 52). No major safety signals were observed but tolerability issues of diarrhea and odor were common (up to 13%). We hypothesized that genetic variants associated with Lp-PLA(2) activity may influence efficacy and tolerability and therefore performed a comprehensive pharmacogenetic analysis of both trials. We genotyped patients within the STABILITY and SOLID-TIMI 52 trials who provided a DNA sample and consent (n = 13,577 and 10,404 respectively, representing 86% and 82% of the trial participants) using genomewide arrays with exome content and performed imputation using a 1000 Genomes reference panel. We investigated baseline and change from baseline in Lp-PLA(2) activity, two efficacy endpoints (major coronary events and myocardial infarction) as well as tolerability parameters at genome-wide and candidate gene level using a meta-analytic approach. We replicated associations of published loci on baseline Lp-PLA2 activity (APOE, CELSR2, LPA, PLA2G7, LDLR and SCARB1) and identified three novel loci (TOMM5, FRMD5 and LPL) using the GWAS-significance threshold P <= 5E-08. Review of the PLA2G7 gene (encoding Lp-PLA(2)) within these datasets identified V279F null allele carriers as well as three other rare exonic null alleles within various ethnic groups, however none of these variants nor any other loci associated with Lp-PLA(2) activity at baseline were associated with any of the drug response endpoints. The analysis of darapladib efficacy endpoints, despite low power, identified six low frequency loci with main genotype effect (though with borderline imputation scores) and one common locus (minor allele frequency 0.24) with genotype by treatment interaction effect passing the GWAS-significance threshold. This locus conferred risk in placebo subjects, hazard ratio (HR) 1.22 with 95% confidence interval (CI) 1.11-1.33, but was protective in darapladib subjects, HR 0.79 ( 95% CI 0.71-0.88). No major loci for tolerability were found. Thus, genetic analysis confirmed and extended the influence of lipoprotein loci on Lp-PLA(2) levels, identified some novel null alleles in the PLA2G7 gene, and only identified one potentially efficacious subgroup within these two large clinical trials.

  • Sundequist Blomdahl, Kristofer
    Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology.
    An evaluation of random-walk based clustering of multiplex networks2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    A network, or a graph, is a mathematical construct used for modeling relationships between different entities. An extension of an ordinary network is a multiplex network. A multiplex network enables one to model different kinds of relationships between the same entities, or even to model how relationships between entities change over time. A common network analysis task is to find groups of nodes that are unusually tightly connected. This is called community detection, and is a form of clustering. The multiplex extension complicates both the notion of what a community is, and the process of finding them.This project focuses on a random-walk based local method that can be used to find communities centered around supplied seed nodes. An implementation of the methodis made which is used to evaluate its ability to detect communities in different kinds of multiplex networks.