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  • Dimberg, Axel
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Thoracic Surgery.
    Alström, Ulrica
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Thoracic Surgery.
    Ståhle, Elisabeth
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Thoracic Surgery.
    Christersson, Christina
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Cardiology.
    Higher Preoperative Plasma Thrombin Potential in Patients Undergoing Surgery for Aortic Stenosis Compared to Surgery for Stable Coronary Artery Disease2018In: Clinical and applied thrombosis/hemostasis, ISSN 1076-0296, E-ISSN 1938-2723, Vol. 24, no 8, p. 1282-1290Article in journal (Refereed)
    Abstract [en]

    Aortic stenosis (AS) and coronary artery disease (CAD) influence the coagulation system, potentially affecting hemostasis during cardiac surgery. Our aim was to evaluate 2 preoperative global hemostasis assays, plasma thrombin potential and thromboelastometry, in patients with severe aortic valve stenosis compared to patients with CAD. A secondary aim was to test whether the assays were associated with postoperative bleeding. Calibrated automated thrombogram (CAT) in platelet-poor plasma and rotational thromboelastometry (ROTEM) in whole blood were analyzed in patients scheduled for elective surgery due to severe AS (n = 103) and stable CAD (n = 68). Patients with AS displayed higher plasma thrombin potential, both thrombin peak with median 252 nmol/L (interquartile range 187-319) and endogenous thrombin potential (ETP) with median 1552 nmol/L/min (interquartile range 1340-1838), when compared to patients with CAD where thrombin peak was median 174 nmol/L (interquartile range 147-229) and ETP median 1247 nmol/L/min (interquartile range 1034-1448; both P < .001). Differences persisted after adjustment for age, gender, comorbidity, and antithrombotic treatment. Differences observed in thromboelastometry between the groups did not persist after adjustment for baseline characteristics. Bleeding amount showed no relationship with plasma thrombin potential but weakly to thromboelastometry (R-2 = .064, P = .001). Patients with AS exhibited preoperatively increased plasma thrombin potential compared to patients with CAD. Plasma thrombin potential was not predictive for postoperative bleeding in patients scheduled for elective surgery.

  • Eriksson, Joakim
    et al.
    RISE SICS.
    Finne, Niclas
    RISE SICS.
    Tsiftes, Nicolas
    RISE SICS.
    Duquennoy, Simon
    RISE SICS.
    Voigt, Thiemo
    Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Computer Architecture and Computer Communication. RISE SICS.
    Scaling RPL to Dense and Large Networks with Constrained Memory2018Conference paper (Refereed)
  • Aaboud, M.
    et al.
    Bergeås Kuutmann, Elin
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Bokan, Petar
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Brenner, Richard
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Ekelöf, Tord
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Ellert, Mattias
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Ferrari, Arnaud
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Gradin, P. O. Joakim
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Isacson, Max
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Mårtensson, Mikael
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Öhman, Henrik
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Sales De Bruin, Pedro
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, High Energy Physics.
    Zwalinski, L.
    Search for flavour-changing neutral current top-quark decays t -> qZ in proton-proton collisions at root s=13 TeV with the ATLAS detector2018In: Journal of High Energy Physics (JHEP), ISSN 1126-6708, E-ISSN 1029-8479, no 7, article id 176Article in journal (Refereed)
    Abstract [en]

    A search for flavour-changing neutral-current processes in top-quark decays is presented. Data collected with the ATLAS detector from proton-proton collisions at the Large Hadron Collider at a centre-of-mass energy of root s = 13TeV, corresponding to an integrated luminosity of 36.1 fb(-1), are analysed. The search is performed using top-quark pair events, with one top quark decaying through the t -> qZ (q = u, c) flavour-changing neutral-current channel, and the other through the dominant Standard Model mode t -> bW. Only Z boson decays into charged leptons and leptonic W boson decays are considered as signal. Consequently, the final-state topology is characterized by the presence of three isolated charged leptons (electrons or muons), at least two jets, one of the jets originating from a b-quark, and missing transverse momentum from the undetected neutrino. The data are consistent with Standard Model background contributions, and at 95% confidence level the search sets observed (expected) upper limits of 1.7 x 10(-4) (2.4 x 10(-4)) on the t -> uZ branching ratio and 2.4 x 10(-4) (3.2 x 10(-4)) on the t -> cZ branching ratio, constituting the most stringent limits to date.

  • Albrektsson, Linda
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Trolle, Hanna
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Är jag en dålig mamma?: Kvinnors upplevelser av förlossningsdepression och sjuksköterskans åtgärder - en litteraturstudie2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    ABSTRACT

    Background: Postpartum depression is a depressive disorder which lasts for a few weeks up to a year after childbirth, and it affects 10 - 15 percent of new mothers. Postpartum depression affects the mother’s quality of life and increases the risk of becoming depressed later in life. This highlights the importance of nurses identifying women with symptoms of postpartum depression and having awareness of existing standards of care.

    Aim: The aim of this literature review was to examine women’s experiences concerning postpartum depression and which standards of care that exist to relieve symptoms of postpartum depression.

    Method: A literature review was conducted with 11 original articles from different countries, which were chosen from the PubMed database. The articles were audited and three categories were developed. The nursing theory of Katie Eriksson was used as theoretical framework in this study.

    Results: There existed a great fear towards becoming stigmatised because of postpartum depression. Many women had trouble acknowledging that they had a problem, which in turn hindered them from seeking help. One aspect which was very important to them was the need for social and professional support. Different standards of care were identified, for example group discussion, mindfulness and yoga. There were also varying educational programmes as well as programmes based on cognitive behavioural therapy.

    Conclusion: Women with postpartum depression feel stigmatised which leads to them not seeking help. Group activities and education concerning postpartum depression had a positive effect on women’s wellbeing. Through active normalisation of postpartum depression and by offering varying standards of care, nurses are able to break the stigma, and receive women with insight and understanding.

  • Johansson, Josefin
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Henriksson, Emma
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Motoriska och kommunikativa utvecklingsavvikelser.: Barnhälsovårdssjuksköterskans bedömning, åtgärder och förutsättningar - en kvalitativ intervjustudie.2018Independent thesis Advanced level (degree of Master (One Year)), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Background: There are differences in how Child health nurses identify developmental disorders. Furthermore, it has been requested evidence-based methods for detection of communicative- and motor disorders.

    Aim: Investigate how motor and communicative disorders for children up to, and including 18 months, are noted in Child health centres. Also, the basis for the assessment, what measures are taken in case of developmental disorders, and if there are any differences in the assessments.

    Method: Descriptive qualitative interview study, based on individual semi-structured interviews, with 12 Child health nurses working at Child health centres in Uppsala Region. The transcriptions were analysed with systematic text condensation by Malterud.

    Result: Child health nurses used intuition when assessing children's development, which they had procured through experience. The clinical examination was described as getting a general picture of the child. The motor and communication development was assessed in whole, due to their related. Continuity was described as meaningful and something that facilitated the assessment of the child. Collegial support was valuable, and the Child health nurses could consult each other, in case of developmental disorders. If the Child health nurses suspected development delay, the child was followed up. The family then often got exercises to practice before the re-visit. In cases where the developmental delay remained, or when the disorder was obvious, the child was referral further. The analysis found that there were individual differences in the assessment done by Child health nurses.

    Conclusions: Today's guidelines focusing on milestones can aggravate the Child health nurse's developmental assessment of children up to and including 18 months. A standardized method, or a screening tool, contributes to an equal and fair Child healthcare. Early detection and early intervention are of great importance to the child's future development.

  • Public defence: 2018-12-17 13:00 Häggsalen, Uppsala
    Edin, Elle
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
    Composite Regenerative Scaffolds2018Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Regenerative medicine and tissue engineering solutions of heavily innervated tissues are at this point lacklustre. This thesis expands our knowledge of appropriate acellular scaffolds for tissue repair in general and nerve regeneration in particular. The optimal surgical procedure for the implantation of artificial extracellular matrix (ECM) was evaluated for recombinant human collagen (RHCIII) implants. Suturing techniques, as well as the usage of human amniotic membrane “bandages” were evaluated. While complete regeneration of corneal tissues occurred, only slight differences in effects of surgical technique could be found.

    The safety and efficacy of clinical trials using mesenchymal stromal cells (MSCs) was evaluated by conducting a systematic review and meta-analysis. MSC therapy was shown to be safe, with no increases mortality, rehospitalization or adverse events. There was also an indication of efficacy, as the overall mortality in the studies included was significantly smaller in the MSC treated group.

    Multicomponent hydrogel capsules encapsulating single cells were developed. Capsules manufactured from gelatin, agarose and fibrinogen were compared to pure gelatin capsules. The composite capsules successfully delayed cell release and prolonged cell survival.

    Surface patterning of collagen based biomimetic corneas was performed by microcontact printing. The ability of different sizes of fibronectin stripes to stimulate cell adhesion and proliferation was compared. The patterned surfaces improved cell adhesion, as well as proliferation markers.

    Conductive polymer composites were manufactured for use as nerve guides. The guides were created from electrospun polycaprolactone fibers coated with a series of different poly(3,4-ethylenedioxythiophene) films. A comparison of nerve progenitor growth and differentiation on the composite fibers was performed. Both the effects of fiber composition and MSC co-culture was investigated, with or without electrostimulation. MSC treatments and polymer coating was both important for nerve cell differentiation and growth.

    List of papers
    1. Effect of Surgical Technique on Corneal Implant Performance.
    Open this publication in new window or tab >>Effect of Surgical Technique on Corneal Implant Performance.
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    2014 (English)In: Translational vision science & technology, ISSN 2164-2591, Vol. 3, no 2, article id 6Article in journal (Refereed) Published
    Abstract [en]

    PURPOSE: Our aim was to determine the effect of a surgical technique on biomaterial implant performance, specifically graft retention.

    METHODS: Twelve mini pigs were implanted with cell-free, 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) cross-linked recombinant human collagen type III (RHCIII) hydrogels as substitutes for donor corneal allografts using overlying sutures with or without human amniotic membrane (HAM) versus interrupted sutures with HAM. The effects of the retention method were compared as well as the effects of collagen concentration (13.7% to 15% RHCIII).

    RESULTS: All implanted corneas showed initial haze that cleared with time, resulting in corneas with optical clarity matching those of untreated controls. Biochemical analysis showed that by 12 months post operation, the initial RHCIII implants had been completely remodeled, as type I collagen, was the major collagenous protein detected, whereas no RHCIII could be detected. Histological analysis showed all implanted corneas exhibited regeneration of epithelial and stromal layers as well as nerves, along with touch sensitivity and tear production. Most neovascularization was seen in corneas stabilized by interrupted sutures.

    CONCLUSIONS: This showed that the surgical technique used does have a significant effect on the overall performance of corneal implants, overlying sutures caused less vascularization than interrupted sutures.

    TRANSLATIONAL RELEVANCE: Understanding the significance of the suturing technique can aid the selection of the most appropriate procedure when implanting artificial corneal substitutes. The same degree of regeneration, despite a higher collagen content indicates that future material development can progress toward stronger, more resistant implants.

    Keywords
    biomaterials, biosynthetic cornea, corneal regeneration, corneal transplantation, recombinant human collagen
    National Category
    Surgery
    Identifiers
    urn:nbn:se:uu:diva-364450 (URN)10.1167/tvst.3.2.6 (DOI)24749003 (PubMedID)
    Available from: 2018-10-29 Created: 2018-10-29 Last updated: 2018-10-29
    2. Conductive PEDOT based coatings on microfibrous scaffolds: a nerve guide component
    Open this publication in new window or tab >>Conductive PEDOT based coatings on microfibrous scaffolds: a nerve guide component
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    2018 (English)Manuscript (preprint) (Other (popular science, discussion, etc.))
    National Category
    Biomaterials Science
    Identifiers
    urn:nbn:se:uu:diva-364452 (URN)
    Available from: 2018-10-29 Created: 2018-10-29 Last updated: 2018-10-29
    3. Mesenchymal Stromal Cells for the Treatment of Ischemic Injury and Vascular Trauma: A Systematic Review and Meta-Analysis
    Open this publication in new window or tab >>Mesenchymal Stromal Cells for the Treatment of Ischemic Injury and Vascular Trauma: A Systematic Review and Meta-Analysis
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    2018 (English)Manuscript (preprint) (Other (popular science, discussion, etc.))
    National Category
    Other Clinical Medicine
    Identifiers
    urn:nbn:se:uu:diva-364451 (URN)
    Available from: 2018-10-29 Created: 2018-10-29 Last updated: 2018-10-29
    4. Functional fabrication of recombinant human collagen-phosphorylcholine hydrogels for regenerative medicine applications.
    Open this publication in new window or tab >>Functional fabrication of recombinant human collagen-phosphorylcholine hydrogels for regenerative medicine applications.
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    2015 (English)In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 12, p. 70-80, article id S1742-7061(14)00486-3Article in journal (Refereed) Published
    Abstract [en]

    The implant-host interface is a critical element in guiding tissue or organ regeneration. We previously developed hydrogels comprising interpenetrating networks of recombinant human collagen type III and 2-methacryloyloxyethyl phosphorylcholine (RHCIII-MPC) as substitutes for the corneal extracellular matrix that promote endogenous regeneration of corneal tissue. To render them functional for clinical application, we have now optimized their composition and thereby enhanced their mechanical properties. We have demonstrated that such optimized RHCIII-MPC hydrogels are suitable for precision femtosecond laser cutting to produce complementing implants and host surgical beds for subsequent tissue welding. This avoids the tissue damage and inflammation associated with manual surgical techniques, thereby leading to more efficient healing. Although we previously demonstrated in clinical testing that RHCIII-based implants stimulated cornea regeneration in patients, the rate of epithelial cell coverage of the implants needs improvement, e.g. modification of the implant surface. We now show that our 500μm thick RHCIII-MPC constructs comprising over 85% water are suitable for microcontact printing with fibronectin. The resulting fibronectin micropatterns promote cell adhesion, unlike the bare RHCIII-MPC hydrogel. Interestingly, a pattern of 30μm wide fibronectin stripes enhanced cell attachment and showed the highest mitotic rates, an effect that potentially can be utilized for faster integration of the implant. We have therefore shown that laboratory-produced mimics of naturally occurring collagen and phospholipids can be fabricated into robust hydrogels that can be laser profiled and patterned to enhance their potential function as artificial substitutes of donor human corneas.

    Keywords
    Collagen, Cornea, Hydrogel, Laser profiling, Surface modification
    National Category
    Biomaterials Science
    Identifiers
    urn:nbn:se:uu:diva-364449 (URN)10.1016/j.actbio.2014.10.035 (DOI)25448347 (PubMedID)
    Available from: 2018-10-29 Created: 2018-10-29 Last updated: 2018-10-29
    5. Controlled Delivery of Human Cells by Temperature Responsive Microcapsules.
    Open this publication in new window or tab >>Controlled Delivery of Human Cells by Temperature Responsive Microcapsules.
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    2015 (English)In: Journal of Functional Biomaterials, ISSN 2079-4983, E-ISSN 2079-4983, Vol. 6, no 2, p. 439-53Article in journal (Refereed) Published
    Abstract [en]

    Cell therapy is one of the most promising areas within regenerative medicine. However, its full potential is limited by the rapid loss of introduced therapeutic cells before their full effects can be exploited, due in part to anoikis, and in part to the adverse environments often found within the pathologic tissues that the cells have been grafted into. Encapsulation of individual cells has been proposed as a means of increasing cell viability. In this study, we developed a facile, high throughput method for creating temperature responsive microcapsules comprising agarose, gelatin and fibrinogen for delivery and subsequent controlled release of cells. We verified the hypothesis that composite capsules combining agarose and gelatin, which possess different phase transition temperatures from solid to liquid, facilitated the destabilization of the capsules for cell release. Cell encapsulation and controlled release was demonstrated using human fibroblasts as model cells, as well as a therapeutically relevant cell line-human umbilical vein endothelial cells (HUVECs). While such temperature responsive cell microcapsules promise effective, controlled release of potential therapeutic cells at physiological temperatures, further work will be needed to augment the composition of the microcapsules and optimize the numbers of cells per capsule prior to clinical evaluation.

    Keywords
    cell delivery, cell encapsulation, human fibroblast, human umbilical vein endothelial cells, hydrogel, microcapsules, temperature responsive
    National Category
    Biomaterials Science
    Identifiers
    urn:nbn:se:uu:diva-364448 (URN)10.3390/jfb6020439 (DOI)26096147 (PubMedID)
    Available from: 2018-10-29 Created: 2018-10-29 Last updated: 2018-10-29
  • Public defence: 2018-12-20 09:15 A1:111a, BMC, Uppsala
    Ahnfelt, Emelie
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
    In vitro evaluation of formulations used in the treatment of hepatocellular carcinoma2018Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Hepatocellular carcinoma (HCC) causes ~ 600,000 deaths annually, making it the second most deadly cancer form. HCC is classified into five stages and for the intermediate HCC treatment, the two most commonly used drug delivery systems (DDSs) are lipiodol-based emulsions and drug-eluting beads. The aims of this thesis were to develop in vitro methods suitable for studying these DDSs. It is important to investigate the release mechanisms and release rates with relevant in vitro methods, as this can improve the understanding of the in vivo performance. Miniaturized in vitro methods with sample reservoirs separated from the release medium by a diffusion barrier were developed and shown to be suitable for studying drug release from particle DDSs (Paper I). In Paper II these methods were further developed and used to study the release of doxorubicin (DOX) from the clinically used drug-eluting beads. DOX release rates were affected by the method set-up and the characteristics of the release medium. The choice of method and volume of release medium could improve the in vivo-likeness of the in vitro release profiles. Applied theoretical models suggested a film-controlled type of DOX release mechanism from the beads when self-aggregation, DOX-bead interaction, and DOX deprotonation were taken into account.

    A micropipette-assisted microscopy method was used to further improve the understanding of the release mechanism of amphiphilic molecules from the beads (Paper III). A detailed analysis suggested an internal depletion-layer model dependent on molecular self-aggregation for the release. It was further suggested that a simple ion-exchange mechanism is unrealistic in physiological conditions.

    The important pharmaceutical factors for the emulsion-based formulations were investigated in Paper IV. DOX solubility, lipid phase distribution, and emulsion stability increased when the contrast agent iohexol was added. Also, an increase in release half-life (h) was observed from emulsions with iohexol.

    The in vitro methods and theoretical models presented in this thesis can be used during development and optimization of future DDSs.

    List of papers
    1. A miniaturized in vitro release method for investigating drug-release mechanisms
    Open this publication in new window or tab >>A miniaturized in vitro release method for investigating drug-release mechanisms
    2015 (English)In: International Journal of Pharmaceutics, ISSN 0378-5173, E-ISSN 1873-3476, Vol. 486, no 1-2, p. 339-349Article in journal (Refereed) Published
    Abstract [en]

    We have evaluated a miniaturized in vitro method, based on the mDISS Profiler (TM) technique that enables on-line monitoring of drug release from a 21 mu l sample with 10 ml of release medium. Four model drugs in eight clinically used formulations, including both solid and non-solid drug delivery systems, were investigated. The acquired data were compared with historical in vitro release data from the same formulations. Use of the Weibull function to describe the in vitro drug-release profiles allowed discrimination between the selected formulations with respect to the drug-release mechanisms. Comparison of the release data from the same formulation in different in vitro set-ups showed that the methodology used can affect the mechanism of in vitro release. We also evaluated the ability of the in vitro methods to predict in vivo activity by comparing simulated plasma concentration-time profiles acquired from the application of the biopharmaceutical software GI-Sim to the in vitro observations. In summary, the simulations based on the miniaturized-method release data predicted the plasma profiles as well as or more accurately than simulations based on the historical release data in 71% of the cases and this miniaturized in vitro method appears to be applicable for both solid and non-solid formulations.

    Keywords
    In vitro release methods, Release mechanisms, Weibull function, GI-Sim, In vivo prediction
    National Category
    Pharmaceutical Sciences
    Identifiers
    urn:nbn:se:uu:diva-255064 (URN)10.1016/j.ijpharm.2015.03.076 (DOI)000353999100037 ()25843760 (PubMedID)
    Available from: 2015-06-22 Created: 2015-06-12 Last updated: 2018-10-30Bibliographically approved
    2. In Vitro Release Mechanisms of Doxorubicin From a Clinical Bead Drug-Delivery System
    Open this publication in new window or tab >>In Vitro Release Mechanisms of Doxorubicin From a Clinical Bead Drug-Delivery System
    2016 (English)In: Journal of Pharmaceutical Sciences, ISSN 0022-3549, E-ISSN 1520-6017, Vol. 105, no 11, p. 3387-3398Article in journal (Refereed) Published
    Abstract [en]

    The release rate of doxorubicin (DOX) from the drug-delivery system (DDS), DC Bead, was studied by 2 miniaturized in vitro methods: free-flowing and sample reservoir. The dependencies of the release mechanisms on in vitro system conditions were investigated experimentally and by theoretical modeling. An inverse relationship was found between release rates and bead size, most likely due to the greater total surface area. The release rates correlated positively with temperature, release medium volume, and buffer strength, although the release medium volume had larger effect than the buffer strength. The sample reservoir method generated slower release rates, which described the in vivo release profile more accurately than the free-flowing method. There was no difference between a pH of 6.3 or 7.4 on the release rate, implying that the slightly acidic tumor microenvironment is less importance for drug release. A positive correlation between stirring rate and release rate for all DDS sizes was observed, which suggests film controlled release. Theoretical modeling highlighted the influence of local equilibrium of protonation, self-aggregation, and bead material interactions of DOX. The theoretical release model might describe the observed larger sensitivity of the release rate to the volume of the release medium compared to buffer strength. A combination of miniaturized in vitro methods and theoretical modeling are useful to identify the important parameters and processes for DOX release from a micro gel-based DDS.

    Keywords
    controlled release, diffusion, dissolution, dissolution rate, drug-delivery systems, in vitro models, mathematical model, microspheres
    National Category
    Pharmaceutical Sciences
    Identifiers
    urn:nbn:se:uu:diva-311211 (URN)10.1016/j.xphs.2016.08.011 (DOI)000388268200018 ()27663384 (PubMedID)
    Funder
    Swedish Research Council, 521-2011-373
    Available from: 2016-12-22 Created: 2016-12-22 Last updated: 2018-10-30Bibliographically approved
    3. Single bead investigation of a clinical drug delivery system – a novel release mechanism
    Open this publication in new window or tab >>Single bead investigation of a clinical drug delivery system – a novel release mechanism
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    2018 (English)In: Journal of Controlled Release, ISSN 0168-3659, E-ISSN 1873-4995, Vol. 292, p. 235-247Article in journal (Other academic) Published
    National Category
    Pharmaceutical Sciences
    Identifiers
    urn:nbn:se:uu:diva-360988 (URN)10.1016/j.jconrel.2018.11.011 (DOI)
    Available from: 2018-09-20 Created: 2018-09-20 Last updated: 2018-11-19
    4. In vitro evaluation of lipiodol-based emulsions in clinical use
    Open this publication in new window or tab >>In vitro evaluation of lipiodol-based emulsions in clinical use
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    (English)Manuscript (preprint) (Other academic)
    National Category
    Pharmaceutical Sciences
    Identifiers
    urn:nbn:se:uu:diva-360987 (URN)
    Available from: 2018-09-20 Created: 2018-09-20 Last updated: 2018-10-30
  • Calatrava Castagnetti, Ruperto Andrés
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Industrial Engineering & Management.
    Coti Zelati, Alberto
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Industrial Engineering & Management.
    Understanding Entrepreneurial Leadership that supports local entrepreneurship2018Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    Leadership and entrepreneurship have often been associated to the ability of guiding ventures, but less to the ability of leading a startup community. This master’s thesis examines literature on entrepreneurship, in particular Entrepreneurial Ecosystem (EE), to understand the role of leadership in sustaining a startup community. Although Stam’s (2015) EE framework recognizes that leadership enables entrepreneurship, it doesn’t define its characteristics and therefore, a more theoretical understanding is demanded. We have thus developed a conceptual framework that aims to facilitate empirical explorations and future analyses of the leadership dimension of Stam’s (2015) EE framework. Our conceptual framework of Entrepreneurial Leadership (EL) includes the following characteristics: collective and bottom-up approach, presence of formal and informal leaders, promotion of social proximity and mentorship driven leadership. The use of interviews as research method and our EL conceptual framework as analytical tool was useful to explore and understand the current state of EL in the startup communities of Amsterdam and Utrecht. On the one hand, the startup community of Amsterdam experiences a shared leadership where formal and informal leaders are recognized. Moreover, the strong presence of supporting organizations, networks of mentors and entrepreneurial events favour interaction among the stakeholders of the EE. On the other hand, Utrecht, a smaller and younger startup community, lacks success entrepreneurial stories that results in supporting organizations exercising the leadership role. Furthermore, there is a poor presence of entrepreneurial events and networks of entrepreneurs that lead to an unconnected community.

  • Senteles, Athanasios
    Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences.
    A GIS-Based Multicriteria Decision Analysis Approach on Wind Power Development: the Case Study of Nova Scotia, Canada2018Independent thesis Advanced level (degree of Master (One Year)), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    The growing need for reducing the negative impacts of climate change and ensuring a constant and environmentally friendly energy supply, led the way to the exploitation of renewable energy sources. Canada has already acknowledged this trend by incorporating more power from renewables on its energy mix. Similarly, Nova Scotia has started an ambitious energy program in which the substitution of most of the fossil fuels by wind energy, will play a significant factor.

    The purpose of this research is to investigate all suitable locations for wind energy development in the province of Nova Scotia, under the scope of minimizing environmental impacts, increasing social acceptance and maximizing energy production. This spatial analysis is performed through the combination of Geographical Information Systems (GIS) and a Multi Criteria Decision Analysis (MCDA).

    The analysis of the province was based on the preferences of wind experts and administration authorities, which formed the weights assigned on eight (8) evaluation criteria. The extract of the relative weights was succeeded by using the Analytical Hierarchy Process (AHP), while their spatial dimensions were expressed by GIS software. The above procedure was possible through the application of a methodology where exclusion areas were found on the first place and the remaining areas were assessed on their level of suitability.

    The implementation of the GIS-MCDA methodological framework indicates that, despite the exclusion of a significant part of the province, there is still enough space to develop wind energy. The applied methodology and relevant results could be used as a Decision-Making tool by planning authorities, wind developers, and stakeholders.

  • Mathers, Adam
    Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences.
    Community Benefit Funds and Wind Power: A Scottish Case Study2018Independent thesis Advanced level (degree of Master (One Year)), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    The Scottish government’s aim of deriving 100 per cent of the nation’s electricity from renewable sources is dependent on the utilisation of wind energy. Social barriers, however, have continued to threaten these targets. Community benefit funds have often been paraded as the most common way of improving public attitudes towards wind farms in the United Kingdom, although little empirical evidence exists to support this notion. Using the proposed Ourack wind farm, approximately three and a half miles north of Grantown-on-Spey in the Scottish Highlands, this case study, consisting of a sequential explanatory research design comprised of an initial close-ended survey followed by in-depth semi-structured interviews, sought to explore the community’s perceptions of community benefits, identify the type of fund that the community wanted, and investigate the role of such benefit provisions in altering perceptions of wind farms. The key findings indicated that the majority of participants were in favour of benefits being provided, they preferred funding to be directed towards community organisations, and approximately one third of research participants (31.6 per cent) perceived the proposed wind farm in a more positive light after considering the possible benefits the region would accrue. Furthermore, the research indicated the need for community benefits to become a standardised part of the planning process, thus reducing the likelihood of financial benefits being perceived as bribes, and allowing developers to provide greater information about any proposed benefits scheme prior to applying for planning permission. There are implications of this study for academics researching the role of community benefits in wind farm planning, and policymakers and developers for understanding the wants and needs of community members.  

  • Roemhild, Roderich
    et al.
    Gokhale, Chaitanya S
    Dirksen, Philipp
    Blake, Christopher
    Rosenstiel, Philip
    Traulsen, Arne
    Andersson, Dan I
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
    Schulenburg, Hinrich
    Cellular hysteresis as a principle to maximize the efficacy of antibiotic therapy.2018In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 115, no 39, p. 9767-9772Article in journal (Refereed)
    Abstract [en]

    Antibiotic resistance has become one of the most dramatic threats to global health. While novel treatment options are urgently required, most attempts focus on finding new antibiotic substances. However, their development is costly, and their efficacy is often compromised within short time periods due to the enormous potential of microorganisms for rapid adaptation. Here, we developed a strategy that uses the currently available antibiotics. Our strategy exploits cellular hysteresis, which is the long-lasting, transgenerational change in cellular physiology that is induced by one antibiotic and sensitizes bacteria to another subsequently administered antibiotic. Using evolution experiments, mathematical modeling, genomics, and functional genetic analysis, we demonstrate that sequential treatment protocols with high levels of cellular hysteresis constrain the evolving bacteria by (i) increasing extinction frequencies, (ii) reducing adaptation rates, and (iii) limiting emergence of multidrug resistance. Cellular hysteresis is most effective in fast sequential protocols, in which antibiotics are changed within 12 h or 24 h, in contrast to the less frequent changes in cycling protocols commonly implemented in hospitals. We found that cellular hysteresis imposes specific selective pressure on the bacteria that disfavors resistance mutations. Instead, if bacterial populations survive, hysteresis is countered in two distinct ways, either through a process related to antibiotic tolerance or a mechanism controlled by the previously uncharacterized two-component regulator CpxS. We conclude that cellular hysteresis can be harnessed to optimize antibiotic therapy, to achieve both enhanced bacterial elimination and reduced resistance evolution.

  • Heritier, K. L.
    et al.
    Imperial Coll London, Dept Phys, Prince Consort Rd, London SW7 2AZ, England.
    Altwegg, K.
    Univ Bern, Phys Inst, Sidlerstr 5, CH-3012 Bern, Switzerland.
    Berthelier, J. -J
    Beth, A.
    Imperial Coll London, Dept Phys, Prince Consort Rd, London SW7 2AZ, England.
    Carr, C. M.
    Imperial Coll London, Dept Phys, Prince Consort Rd, London SW7 2AZ, England.
    De Keyser, J.
    Royal Belgian Inst Space Aeron, BIRA IASB, Ringlaan 3, Brussels, Belgium.
    Eriksson, Anders
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Swedish Institute of Space Physics, Uppsala Division.
    Fuselier, S. A.
    Southwest Res Inst, PO Drawer 28510, San Antonio, TX 78228 USA;Univ Texas San Antonio, San Antonio, TX 78249 USA.
    Galand, M.
    Imperial Coll London, Dept Phys, Prince Consort Rd, London SW7 2AZ, England.
    Gombosi, T. I.
    Univ Michigan, Dept Atmospher Ocean & Space Sci, Ann Arbor, MI 48109 USA.
    Henri, P.
    CNRS, LPC2E, 3 Ave Rech Sci, F-45071 Orleans, France.
    Johansson, Fredrik
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Swedish Institute of Space Physics, Uppsala Division.
    Nilsson, H.
    Swedish Inst Space Phys, POB 812, S-98128 Kiruna, Sweden.
    Rubin, M.
    Univ Bern, Phys Inst, Sidlerstr 5, CH-3012 Bern, Switzerland.
    Wedlund, C. Simon
    Univ Oslo, Dept Phys, Sem Saelands Vei 24Postbox 1048, N-0317 Oslo, Norway.
    Taylor, M. G. G. T.
    European Space Agcy, Estec, Keplerlaan 1, NL-2200 AG Noordwijk, Netherlands.
    Vigren, E.
    LATMOS IPSL CNRS UPMC UVSQ, F-94100 St Maur, France.
    On the origin of molecular oxygen in cometary comae2018In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 9, article id 2580Article in journal (Other academic)
  • Englund, Josefin
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Arts, Department of History.
    Christina Carlsson Wetterberg & Anna Jansdotter (red), Genushistoria, anm av Josefin Nilsson2006In: Historisk Tidskrift (S), ISSN 0345-469X, Historisk Tidskrift, Vol. 126, no 2, p. 359-361Article, book review (Other academic)
  • Borroto-Escuela, Dasiel O.
    et al.
    Karolinska Inst, Dept Neurosci, Retzius Vag 8, S-17177 Stockholm, Sweden;Univ Urbino Carlo Bo, Dept Biomol Sci, I-61029 Urbino, Italy;Observ Cubano Neurociencias, Grp Bohio Estudio, Zaya 50, Yaguajay 62100, Cuba.
    Narvaez, Manuel
    Univ Malaga, Inst Invest Biomed Malaga, Fac Med, E-29071 Malaga, Spain.
    Ambrogini, Patrizia
    Univ Urbino Carlo Bo, Dept Biomol Sci, I-61029 Urbino, Italy.
    Ferraro, Luca
    Univ Ferrara, SVEB, Dept Life Sci & Biotechnol, I-44121 Ferrara, Italy.
    Brito, Ismel
    Karolinska Inst, Dept Neurosci, Retzius Vag 8, S-17177 Stockholm, Sweden;Observ Cubano Neurociencias, Grp Bohio Estudio, Zaya 50, Yaguajay 62100, Cuba.
    Romero Fernandez, Wilber
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Computational Biology and Bioinformatics.
    Andrade-Talavera, Yuniesky
    Karolinska Inst, Dept Neurobiol Care Sci & Soc, Ctr Alzheimer Res, Neuronal Oscillat Lab, S-17177 Stockholm, Sweden.
    Flores-Burgess, Antonio
    Univ Malaga, Inst Invest Biomed Malaga, Fac Med, E-29071 Malaga, Spain.
    Millon, Carmelo
    Univ Malaga, Inst Invest Biomed Malaga, Fac Med, E-29071 Malaga, Spain.
    Gago, Belen
    Univ Malaga, Inst Invest Biomed Malaga, Fac Med, E-29071 Malaga, Spain.
    Angel Narvaez, Jose
    Univ Malaga, Inst Invest Biomed Malaga, Fac Med, E-29071 Malaga, Spain.
    Odagaki, Yuji
    Saitama Med Univ, Dept Psychiat, Saitama 3388570, Japan.
    Palkovits, Miklos
    Semmelweis Univ, Fac Med, Dept Anat Histol & Embryol, H-1094 Budapest, Hungary.
    Diaz-Cabiale, Zaida
    Univ Malaga, Inst Invest Biomed Malaga, Fac Med, E-29071 Malaga, Spain.
    Fuxe, Kjell
    Karolinska Inst, Dept Neurosci, Retzius Vag 8, S-17177 Stockholm, Sweden.
    Receptor-Receptor Interactions in Multiple 5-HT1A Heteroreceptor Complexes in Raphe-Hippocampal 5-HT Transmission and Their Relevance for Depression and Its Treatment2018In: Molecules, ISSN 1420-3049, E-ISSN 1420-3049, Vol. 23, no 6, article id 1341Article, review/survey (Refereed)
    Abstract [en]

    Due to the binding to a number of proteins to the receptor protomers in receptor heteromers in the brain, the term "heteroreceptor complexes" was introduced. A number of serotonin 5-HT1A heteroreceptor complexes were recently found to be linked to the ascending 5-HT pathways known to have a significant role in depression. The 5-HT1A-FGFR1 heteroreceptor complexes were involved in synergistically enhancing neuroplasticity in the hippocampus and in the dorsal raphe 5-HT nerve cells. The 5-HT1A protomer significantly increased FGFR1 protomer signaling in wild-type rats. Disturbances in the 5-HT1A-FGFR1 heteroreceptor complexes in the raphe-hippocampal 5-HT system were found in a genetic rat model of depression (Flinders sensitive line (FSL) rats). Deficits in FSL rats were observed in the ability of combined FGFR1 and 5-HT1A agonist cotreatment to produce antidepressant-like effects. It may in part reflect a failure of FGFR1 treatment to uncouple the 5-HT1A postjunctional receptors and autoreceptors from the hippocampal and dorsal raphe GIRK channels, respectively. This may result in maintained inhibition of hippocampal pyramidal nerve cell and dorsal raphe 5-HT nerve cell firing. Also, 5-HT1A-5-HT2A isoreceptor complexes were recently demonstrated to exist in the hippocampus and limbic cortex. They may play a role in depression through an ability of 5-HT2A protomer signaling to inhibit the 5-HT1A protomer recognition and signaling. Finally, galanin (1-15) was reported to enhance the antidepressant effects of fluoxetine through the putative formation of GalR1-GalR2-5-HT1A heteroreceptor complexes. Taken together, these novel 5-HT1A receptor complexes offer new targets for treatment of depression.

  • Lorant, Tomas
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Transplantation Surgery.
    Bengtsson, Mats
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
    Eich, Torsten
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
    Eriksson, Britt-Marie
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Infectious Diseases.
    Winstedt, Lena
    Hansa Medical AB, Lund, Sweden.
    Järnum, Sofia
    Hansa Medical AB, Lund, Sweden.
    Stenberg, Yvonne
    Hansa Medical AB, Lund, Sweden.
    Robertson, Anna-Karin
    Hansa Medical AB, Lund, Sweden.
    Mosén, Kristina
    Hansa Medical AB, Lund, Sweden.
    Björck, Lars
    Department of Clinical Sciences, Division of Infection Medicine, Lund University, Lund, Sweden.
    Bäckman, Lars
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Transplantation Surgery.
    Larsson, Erik
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical and experimental pathology.
    Wood, Kathryn
    Nuffield Department of Surgical Sciences, Oxford University, Oxford, UK.
    Tufveson, Gunnar
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Transplantation Surgery.
    Kjellman, Christian
    Hansa Medical AB, Lund, Sweden.
    Safety, immunogenicity, pharmacokinetics, and efficacy of degradation of anti-HLA antibodies by IdeS (imlifidase) in chronic kidney disease patients.2018In: American Journal of Transplantation, ISSN 1600-6135, E-ISSN 1600-6143, Vol. 18, no 11, p. 2752-2762Article in journal (Refereed)
    Abstract [en]

    Safety, immunogenicity, pharmacokinetics, and efficacy of the IgG-degrading enzyme of Streptococcus pyogenes (IdeS [imlifidase]) were assessed in a single-center, open-label ascending-dose study in highly sensitized patients with chronic kidney disease. Eight patients with cytotoxic PRAs (median cytotoxic PRAs of 64%) at enrollment received 1 or 2 intravenous infusions of IdeS on consecutive days (0.12 mg/kg body weight ×2 [n = 3]; 0.25 mg/kg ×1 [n = 3], or 0.25 mg/kg ×2 [n = 2]). IgG degradation was observed in all subjects after IdeS treatment, with <1% plasma IgG remaining within 48 hours and remaining low up to 7 days. Mean fluorescence intensity values of HLA class I and II reactivity were substantially reduced in all patients, and C1q binding to anti-HLA was abolished. IdeS also cleaved the IgG-type B cell receptor on CD19+ memory B cells. Anti-IdeS antibodies developed 1 week after treatment, peaking at 2 weeks. A few hours after the second IdeS infusion, 1 patient received a deceased donor kidney offer. At enrollment, the patient had a positive serum crossmatch (HLA-B7), detected by complement-dependent cytotoxicity, flow cytometry, and multiplex bead assays. After IdeS infusion (0.12 mg/kg ×2) and when the HLA-incompatible donor (HLA-B7+ ) kidney was offered, the HLA antibody profile was negative. The kidney was transplanted successfully.

  • Haraldsson, Iza
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Law, Department of Law.
    Incitamentsprogram för ledande befattningshavare: Om utformningen av effektiva incitamentsprogram2018Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
  • Karlsson-Parra, Alex
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology. Immunicum AB, Gothenburg, Sweden.
    Kovacka, Juliana
    Immunicum AB, Gothenburg, Sweden.
    Heimann, Emilia
    Immunicum AB, Gothenburg, Sweden.
    Jorvid, Margareth
    Immunicum AB, Gothenburg, Sweden.
    Zeilemaker, Sijme
    Immunicum AB, Gothenburg, Sweden.
    Longhurst, Sharon
    Immunicum AB, Gothenburg, Sweden.
    Suenaert, Peter
    Immunicum AB, Gothenburg, Sweden.
    Ilixadencel - an Allogeneic Cell-Based Anticancer Immune Primer for Intratumoral Administration2018In: Pharmaceutical research, ISSN 0724-8741, E-ISSN 1573-904X, Vol. 35, no 8, article id UNSP 156Article, review/survey (Refereed)
    Abstract [en]

    Intratumoral administration of an immune primer is a therapeutic vaccine strategy aimed to trigger dendritic cell (DC)-mediated cross-presentation of cell-associated tumor antigens to cytotoxic CD8(+) T cells without the need for tumor antigen characterization. The prevailing view is that these cross-presenting DCs have to be directly activated by pathogen-associated molecular patterns (PAMPS), including Toll-like receptor ligands or live microbial agents like oncolytic viruses. Emerging data are however challenging this view, indicating that the cross-presenting machinery in DCs is suboptimally activated by direct PAMP recognition, and that endogenous inflammatory factors are the main drivers of DC-mediated cross-presentation within the tumor. Here we present preclinical mode of action data, CMC and regulatory data, as well as initial clinical data on ilixadencel. This cell-based drug product is an off-the-shelf immune primer, consisting of pro-inflammatory allogeneic DCs secreting high amounts of pro-inflammatory chemokines and cytokines at the time of intratumoral administration. The mechanism of action of ilixadencel is to induce recruitment and activation of endogenous immune cells, including NK cells that subsequently promotes cross-presentation of cell-associated tumor antigens by co-recruited DCs.

  • Tovar Perez, Alexander Tovar
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health, Research group (Dept. of women´s and children´s health).
    Morphological evaluation of blastocyst after vitrification depending on treatment modality2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Assisted reproductive technology procedures has become a more complex treatment over the years after implementation of preimplantation genetic diagnostics and cryopreservation methods such as slow freeze and vitrification. When embryos undergo these methods they are exposed to external damage that threaten to affect their quality and thereby lead to lower survival rates and lower pregnancy rates.

    The aim of this study was to document blastocysts quality after vitrification, re-vitrification and preimplantation genetic diagnosis with subsequent vitrification.

    A total of 126 blastocysts were collected, of which 119 blastocysts were documented with the help of an experienced embryologists and the remaining seven blastocysts were from a new series of re-vitrified embryos. The 126 collected blastocyst were allocated into groups depending on their degree of preimplantation genetic diagnosis and vitrification. The gathered data was scoring according to morphology, expansion and proportion of necrotic cells at 2 and 4 hours of the expansion phase. Fisher exact test was used for statistical evaluation.

    There were no significant difference when comparing data before and after vitrification and preimplantation diagnosis, which indicates that these methods do not cause morphological damage to the blastocyst.

  • Kristoffersson, Fredrik
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
    Expression of the heparan sulfate biosynthesis enzymes NDST1 and NDST2 and their major splice variants in human tissues.2018Student paper other, 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    The aim of the study was to investigate the expression NDST transcripts in a wide variety of tissues using RNA-sequencing experimental data from five published studies, using two common in silico tools: the Tophat-Cufflink pipeline and the HTSeq-DEXSeq pipeline. We show that to detect NDST alternative transcripts, paired-end sequencing should be used with replicates of samples or conditions together with 100 base read length to allow for reliable detection of the low expressed transcripts in the NDST family.  As a demonstration project, we also characterized HS synthesized by the adrenal carcinoma (ACC) cell line H295R and determined expression of NDSTs in the cells and in ACC tumor samples. We could show that roughly 65% of newly synthesized proteoglycans isolated after metabolic 35S-sulfate labeling of the cells are made up of heparan sulfate (HS) with an average chain length of 45 kDa. The HS chains show a high frequency of N-sulfation and a high total degree of sulfation. Interestingly, disaccharide analysis demonstrated a three-time higher amount of stored chondroitin sulfate (CS) compared to HS in the ACC cell line.

  • Kristoffersson, Fredrik
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
    Novel target genes of ZEB1 and Snail1 in triple-negative human breast cancer.2016Independent thesis Basic level (degree of Bachelor), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    Breast cancer is comprised of several subtypes that are different from one another and thedivergence leads to different outcomes of the disease. There are known prognostic factors andphenotypic distinction in different biological factors and expression patterns, such as theestrogen receptor (ER), progesterone receptor (PR), Ki67, HER2/neu expression (HER2). Ingeneral, there are three breast cancer subtypes with the most recurring subtype being luminalA, and the other two being luminal B and triple negative breast cancer. Triple negative breastcancer is a heterogeneous subtype which is defined by the lack of expression of ERα, PR andHER2. Triple negative breast cancers are also very aggressive and have the worst prognosiscompared to the other two ERα positive tumors. The luminal A subtype can develop into ametastatic cancer thanks to the so-called epithelial-mesenchymal transition (EMT), whichaffects a subpopulation of epithelial cancer cells. EMT is the name of a process that takesplace during the embryonic development, the wound healing and cancer metastasis, where theepithelial cells will transform into mesenchymal cells which have higher invasive andmigratory properties. EMT occurs when epithelial cells lose their apical-basal polarity andthen the adherens- and tight junctions are dissolved. The adherens junction dissolution can beobserved as a downregulation of CDH1 (E-cadherin), which is regularly measured in EMTstudies. Many signaling pathways are associated with the promotion and establishment ofEMT e.g. transforming growth factor β (TGFβ), Notch and Wnt signaling. Bioinformaticscreening was performed to look for mRNA expression levels of ZEB1 and Snail1 indifferent breast cancer cell lines. By using chromatin immunoprecipitation-sequencing (ChIPSeq)in the triple negative (ER-, PR- HER2-) Hs578T breast cancer cell line, a genome-widescreen for ZEB1 and Snail1 binding sites had been performed before the start of the project.The Hs578T cell line expresses many of the EMT transcription factors that are relevant forthe project. Since the signaling of TGFβ is crucial for these genes, manipulation of thissignaling pathway is needed to be able to analyse its importance for the function of thesegenes. To inhibit the activity of TGFβ, the small molecule GW6604 was used to inhibit theTGFβ type I receptor kinase (TβRI) and in that way inhibiting the signaling from thisreceptor. In addition, ZEB1 and Snail1 were knocked out by the use of the transfection andCRISPR/Cas9 knockout technique. By investigating mRNA and protein levels of chosengenes in both control Hs578T cells and ZEB1 and Snail1 knockout Hs578T cells, up or downregulation of some of these genes can be seen with stimulation with TGFβ. The knockout ofSnail1 but not of ZEB1 indicated that the loss of Snail1 generated breast cancer cells thatcould try to revert to epithelial at the phenotypic level.

  • Holmberg, Max
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Signals and Systems Group.
    Development and Evaluation of a Robocentric SLAM Algorithm2018Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    In this master thesis the front end of a Simultaneous Localization And Mapping(SLAM) system is developed in the programming language C++ and themeta-operating system ROS (Robot Operating System). The algorithms are based onprevious work done at the Swedish defense research agency (FOI) and are a part of aGPS free positioning system developed for military use. The parts that have beenimplemented during the project includes: feature extraction from LIDAR data, featureassociation and a Robocentric Extended Kalman Filter. The sensors used in the SLAMsystem are a Velodyne LIDAR (LIght Detection And Ranging) unit and an IMU (InertialMeasurement Unit). During the master thesis, data collection has been done indifferent types of outdoor environments. The resulting front end SLAM with a Kalmanfilter is evaluated in the different types of environments and compared with bothaccurate RTK (Real Time Kinetic) GPS and a version of the filter that uses datafiltered with a GPS. The GPS free SLAM algorithm in urban and forest environmentsgives position estimates that drifts less than 2% compared with the SLAM algorithmthat has help from a GPS. In open field terrain the GPS free SLAM algorithm hastrouble estimating its position due to a lack of features, which results in significantdrift over time. When the SLAM algorithm with GPS filtered data is compared with anaccurate Real Time Kinetic GPS in an urban environment the average drift is less than1%.

  • Werngren, Simon
    Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Division of Systems and Control. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
    Comparison of different machine learning models for wind turbine power predictions2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    The goal of this project is to compare different machine learning algorithms ability to predict wind power output 48 hours in advance from earlier power data and meteorological wind speed predictions. Three different models were tested, two autoregressive integrated moving average (ARIMA) models one with exogenous regressors one without and one simple LSTM neural net model. It was found that the ARIMA model with exogenous regressors was the most accurate while also beingrelatively easy to interpret and at 1h 45min 32s had a comparatively short training time. The LSTM was less accurate, harder to interpretand took 14h 3min 5s to train. However the LSTM only took 32.7s to create predictions once the model was trained compared to the 33min13.7s it took for the ARIMA model with exogenous regressors to deploy.Because of this fast deployment time the LSTM might be preferable in certain situations. The ARIMA model without exogenous regressors was significantly less accurate than the other two without significantly improving on the other ARIMA model in any way

  • Caja, Laia
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Dituri, Francesco
    S de Bellis Res Hosp, Castellana Grotte, Natl Inst Gastroenterol, Bari, Italy.
    Mancarella, Serena
    S de Bellis Res Hosp, Castellana Grotte, Natl Inst Gastroenterol, Bari, Italy.
    Caballero-Diaz, Daniel
    Bellvitge Biomed Res Inst IDIBELL, TGF-β & Canc Grp, Oncobell Program, Gran Via Hosp, Barcelona, Spain; Inst Salud Carlos III, Natl Biomed Res Inst Liver & Gastrointestinal Dis, Oncol Program, CIBEREHD, Madrid, Spain.
    Moustakas, Aristidis
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Giannelli, Gianluigi
    S de Bellis Res Hosp, Castellana Grotte, Natl Inst Gastroenterol, Bari, Italy.
    Fabregat, Isabel
    Bellvitge Biomed Res Inst IDIBELL, TGF-β & Canc Grp, Oncobell Program, Gran Via Hosp, Barcelona, Spain; Inst Salud Carlos III, Natl Biomed Res Inst Liver & Gastrointestinal Dis, Oncol Program, CIBEREHD, Madrid, Spain; Univ Barcelona, Fac Med & Hlth Sci, Dept Physiol Sci, Barcelona, Spain.
    TGF-β and the Tissue Microenvironment: Relevance in Fibrosis and Cancer2018In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 19, no 5, article id E1294Article, review/survey (Refereed)
    Abstract [en]

    Transforming growth factor-β (TGF-β) is a cytokine essential for the induction of the fibrotic response and for the activation of the cancer stroma. Strong evidence suggests that a strong cross-talk exists among TGF-β and the tissue extracellular matrix components. TGF-β is stored in the matrix as part of a large latent complex bound to the latent TGF-β binding protein (LTBP) and matrix binding of latent TGF-β complexes, which is required for an adequate TGF-β function. Once TGF-β is activated, it regulates extracellular matrix remodelling and promotes a fibroblast to myofibroblast transition, which is essential in fibrotic processes. This cytokine also acts on other cell types present in the fibrotic and tumour microenvironment, such as epithelial, endothelial cells or macrophages and it contributes to the cancer-associated fibroblast (CAF) phenotype. Furthermore, TGF-β exerts anti-tumour activity by inhibiting the host tumour immunosurveillance. Aim of this review is to update how TGF-β and the tissue microenvironment cooperate to promote the pleiotropic actions that regulate cell responses of different cell types, essential for the development of fibrosis and tumour progression. We discuss recent evidences suggesting the use of TGF-β chemical inhibitors as a new line of defence against fibrotic disorders or cancer.

  • Yazdi, Homa Papoli
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Evolutionary Biology.
    Ellegren, Hans
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Evolutionary Biology.
    A Genetic Map of Ostrich Z Chromosome and the Role of Inversions in Avian Sex Chromosome Evolution2018In: Genome Biology and Evolution, ISSN 1759-6653, E-ISSN 1759-6653, Vol. 10, no 8, p. 2049-2060Article in journal (Refereed)
    Abstract [en]

    Recombination arrest is a necessary step for the evolution of distinct sex chromosomes. Structural changes, such as inversions, may represent the mechanistic basis for recombination suppression and comparisons of the structural organization of chromosomes as given by chromosome-level assemblies offer the possibility to infer inversions across species at some detail. In birds, deduction of the process of sex chromosome evolution has been hampered by the lack of a validated chromosome-level assembly from a representative of one of the two basal clades of modern birds, Paleognathae. We therefore developed a high-density genetic linkage map of the ostrich Z chromosome and used this to correct an existing assembly, including correction of a large chimeric superscaffold and the order and orientation of other superscaffolds. We identified the pseudoautosomal region as a 52 Mb segment (approximate to 60% of the Z chromosome) where recombination occurred in both sexes. By comparing the order and location of genes on the ostrich Z chromosome with that of six bird species from the other major Glade of birds (Neognathae), and of reptilian outgroup species, 25 Z-linked inversions were inferred in the avian lineages. We defined Z chromosome organization in an early avian ancestor and identified inversions spanning the candidate sex-determining DMRT1 gene in this ancestor, which could potentially have triggered the onset of avian sex chromosome evolution. We conclude that avian sex chromosome evolution has been characterized by a complex process of probably both Z-linked and W-linked inversions (and/or other processes). This study illustrates the need for validated chromosome-level assemblies for inference of genome evolution.

  • Kalliontzi, Eugenia
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
    Effects of acetylation and deacetylation inhibitors on DNA double strand breaks repair and cell survival2018Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    During treatment with ionizing radiation (IR), cells are killed mainly due to ionizing radiation caused acute damage to DNA, such as double-strand breaks, where opposite DNA strands in close proximity are cut. To counteract toxicity of double-strand breaks, cells have evolved DNA damage repair systems such as homologous recombination and non-homologous end joining. An important factor regulating DNA repair after exposure to ionizing radiation is chromatin structure. Interfering with normal functions of chromatin remodelling results in reduced cell survival as seen with histone deacetylase (HDAC) inhibitors such as trichostatin A (TSA) which exhibits antitumoral effects in various cancers. Furthermore, TSA interferes with functions of DNA repair proteins similarly as histone acetyltransferase inhibitors, such as C646, however, the effect of these inhibitors on DNA repair is not fully characterized.

    In this study, DNA damage repair after exposure to TSA and C646 in combination with ionizing radiation was assessed and the results indicated that both TSA and C646 suppressed the survival and proliferation of cancer cells in a dose-dependent manner. When combined with radiation, clonogenic assay shows that C646 radiosensitized HCT116 cells. As measured with flow cytometry, the drugs had no significant effect on the H3K9ac and H3K9me3 histone modifications over the time course of 24 hours. Futhermore, TSA and C646 inhibited ionizing radiation induced foci (IRIF) formation of 53BP1but had no effects on the ability to repair radiation-induced DSBs.

  • Marcon, Alessandro
    et al.
    Univ Verona, Dept Diagnost & Publ Hlth, Unit Epidemiol & Med Stat, Verona, Italy.
    Pesce, Giancarlo
    Univ Verona, Dept Diagnost & Publ Hlth, Unit Epidemiol & Med Stat, Verona, Italy.
    Calciano, Lucia
    Univ Verona, Dept Diagnost & Publ Hlth, Unit Epidemiol & Med Stat, Verona, Italy.
    Bellisario, Valeria
    Univ Turin, Dept Publ Hlth & Pediat, Turin, Italy.
    Dharmage, Shyamali C.
    Univ Melbourne, Sch Populat & Global Hlth, Allergy & Lung Hlth Unit, Melbourne, Vic, Australia.
    Garcia-Aymerich, Judith
    Ctr Res Environm Epidemiol CREAL, ISGlobal, Barcelona, Spain;UPF, Barcelona, Spain;CIBER Epidemiol & Salud PUbl CIBERESP, Barcelona, Spain.
    Gislasson, Thorarinn
    Landspitali Univ Hosp E7, Dept Resp Med & Sleep, Reykjavik, Iceland;Univ Iceland, Fac Med, Reykjavik, Iceland.
    Heinrich, Joachim
    Ludwig Maximilians Univ Munchen, Comprehens Pneumol Ctr Munich, German Ctr Lung Res,Clin Ctr, Inst & Outpatient Clin Occupat Social & Environm, Munich, Germany.
    Holm, Mathias
    Univ Gothenburg, Sahlgrenska Acad, Occupat & Environm Med, Gothenburg, Sweden.
    Janson, Christer
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Lung- allergy- and sleep research.
    Jarvis, Deborah
    Imperial Coll London, NHLI, Populat Hlth & Occupat Dis, London, England;Imperial Coll London, MRC PHE Ctr Environm & Hlth, London, England.
    Leynaert, Benedicte
    Inserm UMR 1152, Pathophysiol & Epidemiol Resp Dis, Paris, France;Univ Paris Diderot Paris 7, UMR 1152, Paris, France.
    Matheson, Melanie C.
    Univ Melbourne, Sch Populat & Global Hlth, Allergy & Lung Hlth Unit, Melbourne, Vic, Australia.
    Pirina, Pietro
    Univ Sassari, Inst Resp Dis, Sassari, Italy.
    Svanes, Cecilie
    Univ Bergen, Ctr Int Hlth, Bergen, Norway;Haukeland Hosp, Dept Occupat Med, Bergen, Norway.
    Villani, Simona
    Univ Pavia, Dept Publ Hlth Expt & Forens Med, Unit Biostat & Clin Epidemiol, Pavia, Italy.
    Zuberbier, Torsten
    Charite Univ Med Berlin, Allergy Ctr Charite, Dept Dermatol & Allergy, Berlin, Germany.
    Minelli, Cosetta
    Imperial Coll London, NHLI, Populat Hlth & Occupat Dis, London, England.
    Accordini, Simone
    Univ Verona, Dept Diagnost & Publ Hlth, Unit Epidemiol & Med Stat, Verona, Italy.
    Trends in smoking initiation in Europe over 40 years: A retrospective cohort study2018In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 13, no 8, article id e0201881Article in journal (Refereed)
    Abstract [en]

    Background: Tobacco consumption is the largest avoidable health risk. Understanding changes of smoking over time and across populations is crucial to implementing health policies. We evaluated trends in smoking initiation between 1970 and 2009 in random samples of European populations.

    Methods: We pooled data from six multicentre studies involved in the Ageing Lungs in European Cohorts consortium, including overall 119,104 subjects from 17 countries (range of median ages across studies: 33-52 years). We estimated retrospectively trends in the rates of smoking initiation (uptake of regular smoking) by age group, and tested birth cohort effects using Age-Period-Cohort (APC) modelling. We stratified all analyses by sex and region (North, East, South, West Europe).

    Results: Smoking initiation during late adolescence (16-20 years) declined for both sexes and in all regions (except for South Europe, where decline levelled off after 1990). By the late 2000s, rates of initiation during late adolescence were still high (40-80 per 1000/year) in East, South, and West Europe compared to North Europe (20 per 1000/year). Smoking initiation rates during early adolescence (11-15 years) showed a marked increase after 1990 in all regions (except for North European males) but especially in West Europe, where they reached 40 per 1000/year around 2005. APC models supported birth cohort effects in the youngest cohorts.

    Conclusion: Smoking initiation is still unacceptably high among European adolescents, and increasing rates among those aged 15 or less deserve attention. Reducing initiation in adolescents is fundamental, since youngsters are particularly vulnerable to nicotine addiction and tobacco adverse effects.

  • Ivert, Torbjörn
    et al.
    Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden;Karolinska Univ Hosp, Heart & Vasc Theme, Stockholm, Sweden.
    Malmström, Håkan
    Karolinska Inst, Inst Environm Med, Unit Epidemiol, Stockholm, Sweden;Swedish Orphan Biovitrum Sobi, Biostat Data Management & Med Writing Res & Dev, Stockholm, Sweden.
    Hammar, Niklas
    Karolinska Inst, Inst Environm Med, Unit Epidemiol, Stockholm, Sweden;AstraZeneca, Global Med Affairs, Med Evidence & Observat Res, Molndal, Sweden.
    Carlsson, Axel C
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Cardiovascular epidemiology. Karolinska Inst, Dept Neurobiol Care Sci & Soc, Div Family Med & Primary Care, Huddinge, Sweden.
    Wändell, Per E.
    Karolinska Inst, Dept Neurobiol Care Sci & Soc, Div Family Med & Primary Care, Huddinge, Sweden.
    Holzmann, Martin J.
    Karolinska Univ Hosp, Dept Emergency Med, Huddinge, Sweden;Karolinska Inst, Dept Internal Med, Solna, Sweden.
    Jungner, Ingmar
    Karolinska Inst, Inst Environm Med, Unit Epidemiol, Stockholm, Sweden.
    Ärnlov, Johan
    Karolinska Inst, Dept Neurobiol Care Sci & Soc, Div Family Med & Primary Care, Huddinge, Sweden;Dalarna Univ, Sch Hlth & Social Studies, Falun, Sweden.
    Walldius, Göran
    Karolinska Inst, Inst Environm Med, Unit Epidemiol, Stockholm, Sweden.
    Cardiovascular events in patients under age fifty with early findings of elevated lipid and glucose levels - The AMORIS study2018In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 13, no 8, article id e0201972Article in journal (Refereed)
    Abstract [en]

    Background: The long-term trajectories of lipid and glucose levels in subjects who experience a major cardiovascular (CV) event at a young age has not been well studied. Our objective was to investigate lipid, lipoprotein, apolipoprotein (apo), and glucose levels in individuals experiencing a CV event before 50 years of age.

    Methods and findings: A first CV event [non-fatal myocardial infarction (MI), coronary revascularisation, or CV related death] before age 50 was recorded in 2,939 (cumulative incidence 1.2% in males and 0.3% in females) of 361,353 individuals included in the prospective Swedish AMORIS (Apolipoprotein-related MOrtality RISk) study with health examinations 1985-1996 and follow-up through 2011. In a nested case-control analysis, cases with a CV event were matched to randomly selected controls. Population risk factor trajectories were calculated up to 20 years prior to an event. Total cholesterol (TC), triglyceride (TG), and glucose levels were higher in cases than in controls as early as 20 years prior to the event with differences increasing over time. Low density lipoprotein, apoB, and the apoB/apoA-1 ratio were higher and increased over time, while HDL and apoA-1 were lower in cases compared to controls. The odds ratio was 2.5 (95% confidence interval 1.6-3.7) for TC >= 5 mmol/L and TG >= 1.7 mmol/L in cases versus controls. The adjusted population-attributable fractions including lipids, glucose, diabetes, smoking, hypertension, and obesity indicated that about 50% of CV events before age 50 may be associated with elevated lipid and glucose levels.

    Conclusions: Elevated TC, TG, LDL, apoB, and glucose levels and high apoB/apo A-1 ratio documented two decades before a CV event in subjects younger than 50 years may account for about half of CV events before age 50, which calls for early recognition and possibly treatment of modifiable CV risk factors in young individuals.

  • Brändas, Erkki J.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Theoretical Chemistry.
    Molecular theory of the genetic code2018In: Molecular Physics, ISSN 0026-8976, E-ISSN 1362-3028, Vol. 116, no 19-20, p. 2622-2632Article in journal (Refereed)
    Abstract [en]

    This article honours Michael Baer on the occasion of his 80th birthday and celebrates his scientific contributions to non-adiabatic chemical physics. This undertaking prompts the presentation of a first principles molecular theory of the genetic code. Jacques Monod's classic essay, 'Chance and Necessity', is exercised as a platform for this discussion. In particular the controversial concept of teleonomy is considered and evaluated in relation to modern developments in chemical physics.

  • Hagström, Hannes
    et al.
    Karolinska Inst, Dept Med, Stockholm, Sweden.
    Andreasson, Anna
    Karolinska Inst, Dept Med, Stockholm, Sweden;Stockholm Univ, Stress Res Inst, Stockholm, Sweden;Macquarie Univ, Dept Psychol, N Ryde, NSW, Australia.
    Carlsson, Axel C
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Cardiovascular epidemiology. Karolinska Inst, Div Family Med & Primary Care, Dept Neurobiol Care Sci & Soc NVS, Stockholm, Sweden.
    Jerkeman, Mats
    Lund Univ, Dept Oncol, Clin Sci, Lund, Sweden.
    Carlsten, Mattias
    Karolinska Inst, Ctr Hematol & Regenerat Med, Dept Med, Stockholm, Sweden.
    Body composition measurements and risk of hematological malignancies: A population-based cohort study during 20 years of follow-up2018In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 13, no 8, article id e0202651Article in journal (Refereed)
    Abstract [en]

    High body mass index (BMI) is associated with development of hematological malignancies (HMs). However, although BMI is a well-established measurement of excess weight, it does not fully reflect body composition and can sometimes misclassify individuals. This study aimed at investigating what body composition measurements had highest association with development of HM. Body composition measurements on 27,557 individuals recorded by healthcare professionals as part of the Malmo Diet and Cancer study conducted in Sweden between 1991-1996 were matched with data from national registers on cancer incidence and causes of death. Cox regression models adjusted for age and sex were used to test the association between one standard deviation increments in body composition measurements and risk of HM. During a median follow-up of 20 years, 564 persons developed an HM. Several body composition measurements were associated with risk of developing an HM, but the strongest association was found for multiple myeloma (MM). Waist circumference (HR 1.31, p = 0.04) and waist-hip ratio (HR 1.61, p = 0.05) had higher risk estimates than BMI (HR 1.18, p = 0.07) for MM. In conclusion, our study shows that measurements of abdominal adiposity better predict the risk of developing HM, particularly MM, compared to BMI.

  • Salar, Dana
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
    Miniature Wave Energy Converter (WEC)2018Independent thesis Advanced level (degree of Master (One Year)), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Abstract

     

     

    In this project, I present a design of a scale model of a linear generator (LG) similar to a full size Wave Energy Converter (WEC) being developed at Uppsala University since 2002 and commercialized by Seabased AB. The purpose of a WEC is to convert the energy from ocean waves into electrical energy.

    In order to implement the behaviour of the prototype design, a preliminary study has been done to further build it for use in education, laboratory tests and research. The challenge with this project is to scale down the WEC but maintain the shape, appearance and characteristics of the generator for educational purposes.

    A miniature version of a WEC, previously developed by Uppsala University in collaboration with Seabased Industry AB, has been designed with scaling rate 1:14 of the linear dimensions. In this case, the value of the output power is not important- it has simply been calculated. The electrical rated parameters of the three phase generator are power  26 W,  peak line-line voltage  13 V and  rated armature current  2 A. The mechanical parameters utilized in the design are the total length and the diameter of the miniature WEC, 50 cm and 25 cm, respectively.

    The simulated prototype model (described in Section 5.4) has been validated with an experimental setup comprising translator and stator (described in Section 5.1), where the translator is moved by a programmed industrial robot. The experimental results have shown good agreement with the simulations.

  • Steffel, Jan
    et al.
    Univ Heart Ctr Zurich, Dept Cardiol, Ramistr 100, CH-8091 Zurych, Switzerland.
    Verhamme, Peter
    Univ Leuven, Dept Cardiovasc Sci, Leuven, Belgium.
    Potpara, Tatjana S.
    Univ Belgrade, Sch Med, Belgrad, Serbia.
    Albaladejo, Pierre
    Grenoble Alps Univ Hosp, Grenoble, France.
    Antz, Matthias
    City Hosp Braunschweig, Braunschweig, Germany.
    Desteghe, Lien
    Hasselt Univ, Fac Med & Life Sci, Hasselt, Belgium.
    Haeusler, Karl Georg
    Charite, Ctr Stroke Res Berlin, Berlin, Germany;Charite, Dept Neurol, Berlin, Germany.
    Oldgren, Jonas
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, UCR-Uppsala Clinical Research Center. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Cardiology.
    Reinecke, Holger
    Univ Hosp Munster, Dept Cardiovasc Med, Munster, Germany.
    Roldan-Schilling, Vanessa
    Univ Murcia, Murcja, Spain.
    Rowell, Nigel
    Sinnaeve, Peter
    Univ Leuven, Dept Cardiovasc Sci, Leuven, Belgium.
    Collins, Ronan
    Tallaght Hosp, Age Related Hlth Care & Stroke Serv, Dublin, Ireland.
    Camm, A. John
    St Georges Univ, Cardiol Clin Acad Grp, Mol & Clin Sci Inst, Londyn, England;Imperial Coll, London, England.
    Heidbüchel, Hein
    Hasselt Univ, Fac Med & Life Sci, Hasselt, Belgium;Antwerp Univ, Antwerpia, Belgium;Univ Hosp, Antwerpia, Belgium.
    The 2018 European Heart Rhythm Association Practical Guide on the use of non-vitamin K antagonist oral anticoagulants in patients with atrial fibrillation: executive summary2018In: Kardiologia polska, ISSN 0022-9032, E-ISSN 1897-4279, Vol. 76, no 9, p. 1283-1298Article in journal (Refereed)
    Abstract [pl]

    Poniższy tekst jest streszczeniem drugiej aktualizacji oryginalnego praktycznego przewodnika opublikowanego w 2013 roku. Leki przeciwkrzepliwe niebędące antagonistami witaminy K (NOAC) stanowią cenną alternatywę dla antagonistów witaminy K (VKA) w zapobieganiu udarom u pacjentów z migotaniem przedsionków (AF) i uznano je za leki preferowane, szczególnie dla osób rozpoczynających leczenie przeciwkrzepliwe. Zarówno lekarze, jak i pacjenci przyzwyczajają się do ich stosowania w praktyce klinicznej, istnieje jednak wiele nierozwiązanych kwestii dotyczących optymalnego stosowania tych leków w określonych sytuacjach klinicznych. Europejskie Stowarzyszenie Zaburzeń Rytmu Serca (EHRA, European Heart Rhythm Association) podjęło się koordynacji opracowania jednolitego sposobu komunikowania się z lekarzami na temat stosowania różnych preparatów NOAC. Grupa określiła 20 tematów zawierających konkretne scenariusze kliniczne, w odniesieniu do których sformułowano praktyczne wskazówki na podstawie dostępnych dowodów. Do problemów klinicznych należą: 1) odpowiednia kwalifikacja pacjentów do leczenia; 2) praktyczne schematy rozpoczynania oraz monitorowania terapii za pomocą NOAC; 3) zagwarantowanie przestrzegania zaleceń przyjmowania doustnych leków przeciwkrzepliwych; 4) zmiana schematów leczenia przeciwkrzepliwego; 5) farmakokinetyka oraz interakcje lekowe; 6) stosowanie NOAC u osób z przewlekłą chorobą nerek i zaawansowaną chorobą wątroby; 7) sposoby pomiaru efektu przeciwkrzepliwego NOAC; 8) pomiar stężenia NOAC w surowicy: rzadkie wskazania, środki ostrożności, potencjalne „pułapki”; 9) postępowanie w przypadku pomyłki w dawkowaniu; 10) postępowanie w przypadku (podejrzenia) przedawkowania bez krwawienia lub badania krzepnięcia wskazujące na potencjalne ryzyko krwawienia; 11) postępowanie w przypadku krwawienia w trakcie terapii za pomocą NOAC; 12) postępowanie u pacjentów poddanych planowym zabiegom chirurgicznym, procedurom inwazyjnym czy ablacji; 13) postępowanie u pacjentów wymagających pilnej interwencji chirurgicznej; 14) pacjenci z AF oraz chorobą wieńcową; 15) unikanie pomyłek w dawkowaniu NOAC w różnych wskazaniach; 16) kardiowersja u pacjenta leczonego NOAC; 17) AF u pacjentów z ostrym udarem mózgu leczonych NOAC; 18) NOAC w sytuacjach szczególnych; 19) leczenie przeciwkrzepliwe w przypadku AF u pacjentów z nowotworami złośliwymi; 20) optymalizacja leczenia za pomocą VKA. Dodatkowe informacje oraz materiały do pobrania, jak również karty leczenia przeciwkrzepliwego w kilku językach można znaleźć na stronie internetowej EHRA (www.NOACforAF.eu).

  • Hellström, Jussi
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Radiology.
    Romanos Zapata, Romina
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Radiology.
    Libard, Sylwia
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical and experimental pathology. Department of Pathology, Uppsala University Hospital, Uppsala, Sweden.
    Wikström, Johan
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Radiology.
    Ortiz-Nieto, Francisco
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Radiology.
    Alafuzoff, Irina
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical and experimental pathology. Department of Pathology, Uppsala University Hospital, Uppsala, Sweden.
    Raininko, Raili
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Radiology.
    The value of magnetic resonance spectroscopy as a supplement to MRI of the brain in a clinical setting2018In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 13, no 11, article id e0207336Article in journal (Refereed)
    Abstract [en]

    BACKGROUND: There are different opinions of the clinical value of MRS of the brain. In selected materials MRS has demonstrated good results for characterisation of both neoplastic and non-neoplastic lesions. The aim of this study was to evaluate the supplemental value of MR spectroscopy (MRS) in a clinical setting.

    MATERIAL AND METHODS: MRI and MRS were re-evaluated in 208 cases with a clinically indicated MRS (cases with uncertain or insufficient information on MRI) and a confirmed diagnosis. Both single voxel spectroscopy (SVS) and chemical shift imaging (CSI) were performed in 105 cases, only SVS or CSI in 54 and 49 cases, respectively. Diagnoses were grouped into categories: non-neoplastic disease, low-grade tumour, and high-grade tumour. The clinical value of MRS was considered very beneficial if it provided the correct category or location when MRI did not, beneficial if it ruled out suspected diseases or was more specific than MRI, inconsequential if it provided the same level of information, or misleading if it provided less or incorrect information.

    RESULTS: There were 70 non-neoplastic lesions, 43 low-grade tumours, and 95 high-grade tumours. For MRI, the category was correct in 130 cases (62%), indeterminate in 39 cases (19%), and incorrect in 39 cases (19%). Supplemented with MRS, 134 cases (64%) were correct, 23 cases (11%) indeterminate, and 51 (25%) incorrect. Additional information from MRS was beneficial or very beneficial in 31 cases (15%) and misleading in 36 cases (17%).

    CONCLUSION: In most cases MRS did not add to the diagnostic value of MRI. In selected cases, MRS may be a valuable supplement to MRI.

  • Svedjedal, Johan
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Arts, Department of Literature, Sociology of Literature.
    Från P.A. Norstedt & Söner till Storytel: Om namn på bokförlag i Sverige2018In: Katharina och namnen: Vänskrift till Katharina Leibring på 60-årsdagen den 20 januari 2018 / [ed] Leila Mattfolk & Kristina Neumüller m.fl., Uppsala: Uppsala universitet, 2018, p. 187-191Chapter in book (Refereed)
  • Garland, Jameson
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Law, Department of Law.
    A Role for Critical Legal Theory in Swedish Family Law?2017In: För barnets bästa: vänbok till Anna Singer / [ed] Margareta Brattström & Maarit Jänterä-Jareborg, Uppsala: Iustus förlag, 2017, p. 111-135Chapter in book (Other academic)
  • Jacobson, Felix
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Wear and degradation of Co, Fe and Ni-based cemented carbides against sandstone and granite2018Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    This diploma work is performed at Sandvik Mining and Rock Technology, Rock Tools division, a world-leading mining equipment manufacturer. The aim is to study the wear of cemented carbide rock drill inserts worn against granite and sandstone countersurfaces, with focus on the impact of binder phase composition and flushing agent chemistry. A brief introduction to rock drilling, cemented carbides and the wear and degradation mechanisms of cemented carbides in rock drilling is given.

    Wear is induced in a lab test by sliding cemented carbide inserts against granite and sandstone countersurfaces while flushing with deionized water or synthetic mine water. The wear is quantified by measuring material weight loss, and worn inserts are studied by SEM.

    All wear marks feature crushed, fragmented and heavily deformed WC grains and cavities left after individually removed WC grains. Rock adhere strongly on inserts worn against both granite and sandstone, and adhered material from both rock types sometimes endure the mechanical contact between insert and rock without significant spalling. Inserts tested with deionized water against sandstone wear 2.2 - 5.1 times more relative to against granite, and the only distinct difference observed is the distribution of adhered rock. The use of synthetic mine water relative to deionized water impacts the wear of all inserts, though corrosion products are only found on some of them. Further, the tribological contact greatly impacts the corrosive attack. The relative wear difference using different water chemistries is larger for inserts with a smaller relative amount of binder. In most cases, increased wear is measured for inserts tested with synthetic mine water. For reasons yet unknown, this trend is reversed for inserts with a high relative amount of binder tested against sandstone, where a decrease in wear is measured instead.

  • Public defence: 2018-12-17 13:15 Hörsal 2, 753 13 Uppsala
    Brandén, Gunnar
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Social Sciences, Department of Economics.
    Understanding Intergenerational Mobility: Inequality, Student Aid and Nature-Nurture Interactions2018Doctoral thesis, monograph (Other academic)
    Abstract [en]

    Essay I: A body of evidence has emerged in the literature on intergenerational mobility documenting that unequal countries experience less social mobility: a relationship known as the Great Gatsby Curve. In this paper I estimate the Great Gatsby Curve within Sweden across 125 commuting zones and 20 cohorts, exploiting both cross-sectional and longitudinal variation. I find that children who were exposed to higher levels of inequality during childhood experienced less social mobility as adults, thereby confirming the existence of a Great Gatsby Curve in Sweden. I also present new evidence on the underlying mechanisms of the Great Gatsby Curve. By decomposing intergenerational mobility into separate transmission channels, I find that the Great Gatsby Curve is exclusively driven by the mediating effect that children's educational attainment and development of cognitive and non-cognitive skills has on the persistence of income across generations. Hence, the results suggest that adverse effects of inequality on mobility can be alleviated by policies that target children's educational attainment and development of cognitive and non-cognitive skills.

  • Olsson, Fredrik
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Division of Systems and Control. Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Automatic control.
    Medvedev, Alexander
    Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Division of Systems and Control. Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Automatic control.
    Tremor Severity Rating by Markov Chains2018Conference paper (Refereed)
    Abstract [en]

    The paper deals with mathematical modeling tools for tremor quantification, a problem arising in e.g. clinical applications and sports. Tremor is an involuntary repetitive movement of extremities, head, or trunk that occurs in disease but also in health, due to e.g. strain or fatigue. Quantification of tremor is traditionally performed by ocular observation, while numerous technologies based on wearable accelerometer data exist and have been tested in medical practice. The currently available approaches rely on spectral analysis that reduces a fundamentally nonlinear and non-stationary phenomenon to a linear combination of harmonic components. The classical nonlinear identification methods are as well of limited use because the underlying system is essentially autonomous and produces sustained oscillations without exogenous excitation. An alternative view on tremor is therefore adopted that treats the problem from a severity rating perspective aligned with clinical practices. The tremor amplitude is modelled by a Markov chain, where the states represent the predefined intervals of severity. A comparison with a previously developed event-based method of tremor quantification is provided on data collected using a smart phone in a patient diagnosed with Parkinson disease and undergoing Deep Brain Stimulation therapy. The experimental procedure is unobtrusive and can be implemented in a way that is transparent to the patient.

  • Nilsson, Josefine
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Nilsson, Frida
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Vuxna patienter med psykisk ohälsa: Upplevelser av sjuksköterskors bemötande på akutmottagning: En litteraturstudie2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Background: Mental health is a public health problem that could be found in different ages and has big consequences for the individual ́s quality of life. One problematic aspect is the ongoing stigmatisation in the society. Previous research has shown difficulties for mental health patients to have contact with health care facilities. In the emergency department, the physical illness is a priority. Therefore, the health care is perceived as insufficient for patients with mental health problems.

    Aim: The aim of this study was to explore the experiences of adults with mental health issues, regardless of the nature of the health seeking reason, and how they perceive that they get treated by the nurses in the emergency departments.

    Method: It ́s a literature research based on 13 empirical articles. The databases that has been used to find relevant articles was PubMed and PsycINFO. A content analysis was made on the articles. Joyce Travelbees nursing theory was used as a theoretical framework for this study.

    Result: Five categories was identified explaining experiences of nurses ́ treatment in the emergency department among patients with mental health issues. The catagories were: positive experiences, perceived shortcomings, individual difficulties, external impact factors and requests for equal treatment. The most problematic aspects of the nurses ́ patient treatment were lack of involvement and a judgemental behavior. Physical health was also considered as more important than mental health.

    Conclusion: Shortcomings were found in the nurse ́s treatment of patients with mental health issues in the emergency departments. The problematic factor is the stigmatisation of mental health. For that reason, new research and more knowledge is required to highlight and find solutions to the problem.

  • Assenova, Daniela
    et al.
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Languages, Department of Modern Languages, Slavic Languages.
    Svensson, MyUppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Languages, Department of Modern Languages, Slavic Languages.
    Slovo. Journal of Slavic Languages and Literatures, No. 50, 20102010Collection (editor) (Refereed)
  • Nuorluoto, Juhani
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Languages, Department of Modern Languages, Slavic Languages.
    Central Slovak and Kajkavian Structural Convergences: A Tentative Survey2010In: Slovo : Journal of Slavic Languages and Literatures, ISSN 0348-744X, E-ISSN 2001-7359, Vol. 50, p. 37-45Article in journal (Refereed)
    Abstract [en]

    The purpose of this tentative survey is to shed light on certain structural features that are common to the central Slovak and the Croatian kajkavian dialects. Some of these elements may be parallel developments, but there is a number of features that inevitably testify to the former existence of a continuum in the area that became largely de-Slavicized after the arrival of the Magyars, on the one hand, and the Bavarian Germans, on the other. It should be emphasized that this survey is preliminary, based on fieldwork carried out by the author, and that the features described below may also occur elsewhere. I tentatively designate the identified common isoglosses as “Pannonian.” 

  • Assenova, Daniela
    To our reviewers2010In: Slovo : Journal of Slavic Languages and Literatures, ISSN 0348-744X, E-ISSN 2001-7359, Vol. 51, p. 149-Article in journal (Other (popular science, discussion, etc.))
  • Renström, Terese
    Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences.
    Kartläggning av metallflöden i avloppsvatten i Västerås2018Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    Sludge, produced in the waste water treatment process, can be used as fertilizer in agriculture. It is rich in nutrients but also contains other substances, such as heavy metals. Metals may enter crops which means that it also enters the food chain. Some heavy metals have been proven to cause severe damage to living organisms in high doses. It is therefore important to regulate the amount of heavy metals in the soil and in the sludge used as fertilizer. In this thesis the sources of heavy metals in the waste water system in the town of Västerås was examined. The sources of the heavy metals cadmium, chromium, mercury, copper and zinc were mainly analyzed by using the Excel tool "Source Finder". In this tool collected data of emissions or calculated emissions by the use of model values were entered. Households proved to be the single largest source of all the metals in this study, with the exeption of chromium. For cadmium and chromium water leakage from the ground water into the pipe system was a large contributing source. Business did not prove to be a large source of any metal with the exception of dental units which emitted large amounts of mercury. To be able to predict change of quality, in regards of metals, in the sludge and puried water an existing model of the water treatment plant was supplemented with processes regarding separation of copper. This was done by studying other models regarding metal partitioning and separation. The final model proved unable to describe variances in the measured data, but could describe the median concentration of copper in cleansed water and sludge.

  • Assenova, Daniela
    et al.
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Languages, Department of Modern Languages, Slavic Languages.
    Lysén, IrinaUppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Languages, Department of Modern Languages, Slavic Languages.Svensson, MyUppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Languages, Department of Modern Languages, Slavic Languages.
    Slovo. Journal of Slavic Languages and Literatures, No. 51, 20102010Collection (editor) (Refereed)
  • To our Reviewers2011In: Slovo : Journal of Slavic Languages and Literatures, ISSN 0348-744X, E-ISSN 2001-7359, Vol. 52, p. 115-Article in journal (Other (popular science, discussion, etc.))
  • News from Uppsala2011In: Slovo : Journal of Slavic Languages and Literatures, ISSN 0348-744X, E-ISSN 2001-7359, Vol. 52, p. 113-114Article in journal (Other (popular science, discussion, etc.))
  • Qi, Xiaoying
    et al.
    Binzhou Med Univ, Med & Pharm Res Ctr, Yantai, Peoples R China.
    Zhang, Yunyun
    Yantai Zestern Biotech Co LTD, Yantai, Peoples R China.
    Zhang, Yuan
    Binzhou Med Univ, Med & Pharm Res Ctr, Yantai, Peoples R China.
    Ni, Tianhui
    Binzhou Med Univ, Precis Med Res Ctr, Yantai, Shandong, Peoples R China.
    Zhang, Wenfeng
    Yantai Zestern Biotech Co LTD, Yantai, Peoples R China.
    Yang, Chunhua
    Binzhou Med Univ, Med & Pharm Res Ctr, Yantai, Peoples R China.
    Mi, Jia
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. Binzhou Med Univ, Med & Pharm Res Ctr, Yantai, Peoples R China.
    Zhang, Jiandi
    Yantai Zestern Biotech Co LTD, Yantai, Peoples R China;Binzhou Med Univ, Precis Med Res Ctr, Yantai, Shandong, Peoples R China.
    Tian, Geng
    Binzhou Med Univ, Med & Pharm Res Ctr, Yantai, Peoples R China.
    High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)2018In: Journal of Visualized Experiments, ISSN 1940-087X, E-ISSN 1940-087X, no 138, article id e56885Article in journal (Refereed)
    Abstract [en]

    Lacking a convenient, quantitative, high throughput immunoblot method for absolute determination of the content of a specific protein at cellular and tissue level significantly hampers the progress in proteomic research. Results derived from currently available immunoblot techniques are also relative, preventing any efforts to combine independent studies with a large-scale analysis of protein samples. In this study, we demonstrate the process of quantitative dot blot analysis (QDB) to achieve absolute quantification in a high throughput format. Using a commercially available protein standard, we are able to determine the absolute content of capping actin protein, gelsolin-like (CAPG) in protein samples prepared from three different mouse tissues (kidney, spleen, and prostate) together with a detailed explanation of the experimental details. We propose the QDB analysis as a convenient, quantitative, high throughput immunoblot method of absolute quantification of individual proteins at the cellular and tissue level. This method will substantially aid biomarker validation and pathway verification in various areas of biological and biomedical research.

  • Hoffner, Mattias
    et al.
    Lund Univ, Dept Clin Sci, Malmo, Sweden;Blekinge Hosp, Dept Surg, Karlskrona, Sweden.
    Ohlin, Karin
    Skane Univ Hosp, Dept Plast & Reconstruct Surg, Jan Waldemstroms Gata 18, SE-20502 Malmo, Sweden.
    Svensson, Barbro
    Skane Univ Hosp, Dept Plast & Reconstruct Surg, Jan Waldemstroms Gata 18, SE-20502 Malmo, Sweden.
    Manjer, Jonas
    Lund Univ, Dept Clin Sci, Malmo, Sweden;Skane Univ Hosp, Dept Plast & Reconstruct Surg, Jan Waldemstroms Gata 18, SE-20502 Malmo, Sweden.
    Hansson, Emma
    Lund Univ, Dept Clin Sci, Malmo, Sweden;Sahlgrens Univ Hosp, Dept Plast & Reconstruct Surg, Gothenburg, Sweden.
    Troëng, Thomas
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Vascular Surgery. Uppsala Univ Hosp, Surg Vasc Sect, Uppsala, Sweden.
    Brorson, Hakan
    Lund Univ, Dept Clin Sci, Malmo, Sweden;Skane Univ Hosp, Dept Plast & Reconstruct Surg, Jan Waldemstroms Gata 18, SE-20502 Malmo, Sweden.
    Liposuction Gives Complete Reduction of Arm Lymphedema following Breast Cancer Treatment: A 5-year Prospective Study in 105 Patients without Recurrence2018In: Plastic and reconstructive surgery (1963), ISSN 0032-1052, E-ISSN 1529-4242, Vol. 6, no 8, article id e1912Article in journal (Refereed)
    Abstract [en]

    Background: Arm lymphedema is a well-recognized complication after breast cancer surgery that negatively impacts patients' quality of life, both physiologically and psychologically. Lymph stasis and inflammation result in excess formation of adipose tissue, which makes removal of the deposited subcutaneous fat necessary to eliminate the excess volume. Liposuction, combined with postoperative controlled compression therapy (CCT), is the only treatment that gives complete reduction of the excess volume. The aim of this study was to evaluate the 5-year results after liposuction in combination with CCT. Methods: Patients consecutively operated on between 1993 and 2012 were identified from the lymphedema registry, comprising all patients with nonpitting lymphedema treated with liposuction and CCT in our department. Standardized forms were used to collect pre-, peri-, and postoperative data. Results: One hundred five women with nonpitting edema were treated. The mean interval between the breast cancer operation and lymphedema start was 2.95.0 years, the mean duration of lymphedema was 10 +/- 7.4 years, and the preoperative mean excess volume was 1,573 +/- 645ml. The mean volume aspirated was 1,831 +/- 599ml. Postoperative mean reduction 5 years postoperatively was 117% +/- 26% as compared with the healthy arm. Conclusion: Liposuction is an effective method for the treatment of chronic, nonpitting, arm lymphedema resistant to conservative treatment. The volume reduction remains complete after 5 years.

  • Assenova, Daniela
    et al.
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Languages, Department of Modern Languages, Slavic Languages.
    Lysén, IrinaUppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Languages, Department of Modern Languages, Slavic Languages.Nuorluoto, JuhaniUppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Languages, Department of Modern Languages, Slavic Languages.
    Slovo. Journal of Slavic Languages and Literatures, No. 52, 20112011Collection (editor) (Refereed)
  • Public defence: 2018-12-14 13:15 Sal IV, Universitetshuset, Uppsala
    Alm Fjellborg, Andreas
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Social Sciences, Department of Social and Economic Geography. Uppsala University.
    Housing tenure and residential mobility in Stockholm 1990-20142018Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    In this thesis the links between housing tenure, income and selective, segregation generating, residential mobility are explored. The development of these links is analysed against the background of housing regime changes in Stockholm between 1990 and 2014. Housing policy changes in Sweden, and Stockholm, promote ownership through, for instance, housing tenure conversions and the tax-system. What this development means for residential mobility trends and may mean for ethnic and economic segregation is explored in three articles. Paper 1 contrasts two time periods and provides an analysis of residential mobility and economic sorting. It is shown that the socioeconomic composition of movers is relatively stable over time while the increasingly owner dominated housing market in Stockholm contributes to stronger socio-spatial residential patterns through the strengthened economic sorting of movers across the whole income scale. Paper 2 has a focus on ethnic and socioeconomic differences in out-mobility from poor neighbourhoods. The findings indicate that foreign background residents are dependent upon housing wealth and income to be able to leave poor neighbourhoods when they move, while the Swedish background group has a variety of resources at their disposal when they move. Paper 3 analyses how housing tenure affects moving, and movers' destinations, in neighbourhoods with high concentrations of non-western foreign-born residents in Stockholm. By comparing two cohorts (1993-2000 and 2001-2008) it is analysed how this relationship develops over time. Housing tenure and income do not seem to be pivotal for who moves, but increasingly important for where movers end up. Results display ethnic differences and how the changing housing market in Stockholm reproduces ethnic segregation. Two main conclusions from the thesis are that (i) the changing housing regime in Stockholm produces stronger economic sorting of movers – this has been affected by the geographically, socioeconomically and ethnically uneven gains from the housing market transformations experienced over the last three decades. (ii) The residential mobility patterns and the economic stratification of residential mobility opportunities that the reconfiguration of Stockholm’s housing market gives rise to increases the economic, political and social marginalization of neighbourhoods characterized by low income levels and high shares of foreign-born residents. Combating ethnic segregation is today even more closely related to the socioeconomic differences between the foreign-born and native-born parts of the population.

    List of papers
    1. Residential mobility and spatial sorting in Stockholm 1990-2014: The changing importance of housing tenure and income
    Open this publication in new window or tab >>Residential mobility and spatial sorting in Stockholm 1990-2014: The changing importance of housing tenure and income
    (English)Manuscript (preprint) (Other academic)
    Abstract [en]

    In this paper an analysis of residential mobility and sorting by income in Stockholm is carried out. The study contrasts two periods: one (1990-2001) characterised by recovery after the economic crisis in 1991-1992 and one (2002-2014) characterised by large changes to the housing stock composition. With the use of a longitudinal full population data set, it is shown that the socioeconomic composition of movers is relatively stable over time, while the economic sorting of movers increases. People with low income are increasingly likely to move into low-income neighbourhoods, whether they move into the rental or owner segments of the housing market. Those who own their housing unit and mid- to high-income earners increasingly avoid moving to low-income neighbourhoods. The paper concludes that the increasingly owner-dominated housing market in Stockholm contributes to stronger socio-spatial residential patterns through the economic sorting of movers across the whole income scale. 

    Keywords
    Residential mobility, housing tenure, spatial sorting, Stockholm
    National Category
    Human Geography
    Research subject
    Social and Economic Geography
    Identifiers
    urn:nbn:se:uu:diva-363443 (URN)
    Available from: 2018-10-24 Created: 2018-10-24 Last updated: 2018-10-26
    2. Leaving poor neighbourhoods: the role of income and housing tenure
    Open this publication in new window or tab >>Leaving poor neighbourhoods: the role of income and housing tenure
    (English)Manuscript (preprint) (Other academic)
    Abstract [en]

    To date, few studies have adopted a particular focus on the role of housing tenure when analysing ethnic and socioeconomic differences in out-mobility from poor neighbourhoods. This study contributes to filling this gap. The paper uses a full population data set covering every individual residing in the capital region of Sweden during the period 2006-2008. The findings indicate that those with foreign background are dependent on housing wealth and higher income to be able to leave poor neighbourhoods when moving, while native Swedes seem to be less constrained by income. The results warrant efforts to broaden mix policy beyond the discussion on housing tenure if policy-makers want to counteract the ethnic and socioeconomic imbalances of residential mobility upholding segregation.

    Keywords
    selective mobility, poor neighbourhoods, segregation, housing tenure, Stockholm
    National Category
    Human Geography
    Identifiers
    urn:nbn:se:uu:diva-364228 (URN)
    Available from: 2018-10-24 Created: 2018-10-24 Last updated: 2018-10-26
    3. Out-mobility from Stockholm’s foreign-born concentration neighbourhoods – a study of two cohorts
    Open this publication in new window or tab >>Out-mobility from Stockholm’s foreign-born concentration neighbourhoods – a study of two cohorts
    (English)Manuscript (preprint) (Other academic)
    Abstract [en]

    In this study the central theme is how housing tenure affects moving from neighbourhoods with high concentrations of non-western foreign-born residents in Stockholm and, by comparing two cohorts (1993-2000 and 2001-2008), how this relationship between housing tenure, ethnic background and residential mobility develops over time. Attention is also given to the sorting of movers. The findings disclose that housing tenure’s effect on moving marginally varies between neighbourhood types, and earlier existing differences between housing tenures are reduced over time. Out-mobility destination data suggest that owning is important, and increasingly important, for moving towards areas with fewer non-western foreign-born residents. The findings show that housing tenure mix as a policy tool to counteract high mobility frequencies in distressed neighbourhoods is losing strength. Results indicate how the increasingly owner-dominated housing market in Stockholm reproduces ethnic segregation through stronger residential sorting by income and housing tenure. 

    National Category
    Human Geography
    Identifiers
    urn:nbn:se:uu:diva-364255 (URN)
    Available from: 2018-10-24 Created: 2018-10-24 Last updated: 2018-10-26
  • Jansson, Lisa
    Uppsala University, Disciplinary Domain of Humanities and Social Sciences, Faculty of Arts, Department of ALM.
    Museibutikens förutsättningar: Policy och praktik bland museibutiker i Stockholm2018Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    The purpose of this master’s thesis was to investigate how museum stores are governed, with a focus on museum stores situated in museums in Stockholm and on museums stores which are part of a government agency. The purpose was also to investigate the relationship between the museum and the consumption in the museum store in general. 

    The thesis shows that museum stores which are part of a government agency are governed by their appropriation directions, including the direction that the museum stores must be fully financed. However, the museum stores are also influenced by guidelines that have not been written down, expectations from the museum and from museum patrons. Other factors not within the control of the museum were also of importance, and could limit the choices available for the museum store managers as well as be a source of inspiration. The result also shows that the museum store and the consumption it’s associated with, is seen as either a threat, a necessity or a possibility: The museum store is often viewed as being at odds with the main role of museums in general, while also being considered a necessity, helping the museum to meet the patron’s expectations. In some cases the museum could benefit from the museum store, due to the stores’ possibility of improving upon the museum visit, or making a profit usable in other museum activities.

    The source material was gathered from the websites of international and national organisations within the museum field. Material was also gathered during interviews with museum store managers in Stockholm. The theory used was cultural economics and consumption studies. The method for this essay was interviews, minor field studies, linguistic analysis. The focus of this essay was how the museum store is governed. The essay does not, for example, research how the museum visitors or museum patrons experienced the shop.

    This is a two years master’s thesis in Museum and Cultural Heritage Studies