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Sand, Lars
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Jalouli, M. M., Jalouli, J., Öhman, J., Hasseús, B., Hirsch, J. M. & Sand, L. (2015). Association of Human Papilloma Virus infection in healthy oral mucosa, oral dysplasia and oral squamous cell carcinoma. Journal of Oral Health and Dental Management, 14(5)
Åpne denne publikasjonen i ny fane eller vindu >>Association of Human Papilloma Virus infection in healthy oral mucosa, oral dysplasia and oral squamous cell carcinoma
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2015 (engelsk)Inngår i: Journal of Oral Health and Dental Management, ISSN 1583-5588, Vol. 14, nr 5Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Aims: Human papillomavirus (HPV) is an important risk factor for development of oral cancer; however, the integrational status of the virus into the host DNA association between HPV infection and oral squamous cell carcinoma (OSCC) is still uncertain has not been investigated to the same extent. The objective of the present study was to investigate the prevalence of consensus HPV, and HPV-16 and its integration status, in healthy oral mucosa, oral epithelial dysplasia (OED), and OSCC samples.Materials and Methods: The study material consisted of 26 fresh, normal oral mucosa samples, and 53 and 27 paraffin-embedded OED and OSCC samples, respectively. The specimens were DNA extracted and investigated for the presence of HPV, and HPV-16 and its integration status, by polymerase chain reaction (PCR) and DNA sequencing.Results: Thirty-eight (72%) of the 53 paraffin-embedded OED samples, 16 (59%) of the 27 OSCC samples, and 12 (46%) of the 26 control samples were found to be HPV-DNA positive, with nested PCR (NPCR). Further, HPV-16 was detected in 31 (82%), 15 (94%), and 0 (0%) HPV-positive OED cases, HPV-positive OSCC cases, and controls, respectively. Integration was observed in 26/31 (84%) and 13/15 (87%) of the HPV-16-positive OED and OSCC cases, respectively. A statistically significant difference was found comparing prevalence of HPV-16 in controls with that in both OED and OSCC samples (P<0.005). A statistically significant difference was also found comparing prevalence of integrated and episomal viral forms comparing OED and OSCC samples (P<0.005).Conclusion: The high prevalence of HPV and high-risk HPV-16 in OED and OSCC samples suggests a potential aetiologic role for the virus in OSCC.

HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-265748 (URN)
Tilgjengelig fra: 2015-11-03 Laget: 2015-11-03 Sist oppdatert: 2017-12-01
Jalouli, M., Jalouli, J., Ibrahim, S. O., Hirsch, J.-M. & Sand, L. (2015). Comparison Between Single PCR and Nested PCR in Detection of Human Papilloma Viruses in Paraffin-embedded OSCC and Fresh Oral Mucosa. In Vivo, 29(1), 65-70
Åpne denne publikasjonen i ny fane eller vindu >>Comparison Between Single PCR and Nested PCR in Detection of Human Papilloma Viruses in Paraffin-embedded OSCC and Fresh Oral Mucosa
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2015 (engelsk)Inngår i: In Vivo, ISSN 0258-851X, E-ISSN 1791-7549, Vol. 29, nr 1, s. 65-70Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background: Infection with human papilloma virus (HPV) has been implicated as one of the risk factors for the development of oropharyngeal cancer. Many different HPV tests exist, and information regarding their specific technical, analytical, and clinical properties is increasing. Aim: This study aimed to compare the level of detection of HPV using two reliable polymerase chain reaction (PCR) methods, nested PCR (NPCR) and single PCR (SPCR), in archival paraffin-embedded oral squamous cell carcinoma (OSCC) samples and fresh oral mucosa specimens. Materials and Methods: The presence of HPV genome in two groups of tissue samples was analyzed: (i) 57 paraffin-embedded OSCC samples from Sudan and (ii) eight healthy fresh oral mucosal samples from Swedish volunteers. The specimens were tested by SPCR with primer pair MY9/MY11 and NPCR using GP5+/GP6+ primer sets. Results: Eighteen (32%) out of the 57 paraffin-embedded OSCC samples, and five (62%) out of the eight fresh clinically healthy samples were found to be HPV-positive with NPCR. With SPCR, four (7%) out of the paraffin-embedded OSCC samples were HPV-positive. A statistically significant difference between HPV-positive and -negative samples was found when comparing NPCR and SPCR in OSCC and fresh oral mucosa (p<0.0001). The comparative test between SPCR and NPCR showed 100% sensitivity and 69% specificity for OSCC. Conclusion: The use of the GP5+/GP6+ nested PCR increased the positivity rate, efficiency rate and sensitivity of HPV detection in oral samples significantly and should be considered as the method of choice.

Emneord
HPV, nested PCR, single PCR, paraffin embedded, OSCC, fresh oral mucosa
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-245198 (URN)000348134300011 ()25600532 (PubMedID)
Tilgjengelig fra: 2015-02-25 Laget: 2015-02-25 Sist oppdatert: 2017-12-04bibliografisk kontrollert
Jalouli, M., Jalouli, J., Hasséus, B., Öhman, J., Hirsch, J. M. & Sand, L. (2015). Nested PCR for detection of HSV-1 in oral mucosa. Medicina Oral, 20(6), E664-E669
Åpne denne publikasjonen i ny fane eller vindu >>Nested PCR for detection of HSV-1 in oral mucosa
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2015 (engelsk)Inngår i: Medicina Oral, ISSN 1698-4447, E-ISSN 1698-6946, Vol. 20, nr 6, s. E664-E669Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

BACKGROUND: It has been estimated that 15%-20% of human tumours are driven by infection and inflammation, and viral infections play an important role in malignant transformation. The evidence that herpes simplex virus type 1 (HSV-1) could be involved in the aetiology of oral cancer varies from weak to persuasive. This study aimed to investigate by nested PCR (NPCR) the prevalence of HSV-1 in samples from normal oral mucosa, oral leukoplakia, and oral squamous cell carcinoma (OSCC).

MATERIAL AND METHODS: We investigated the prevalence of HSV-1 in biopsies obtained from 26 fresh, normal oral mucosa from healthy volunteers as well as 53 oral leukoplakia and 27 OSCC paraffin-embedded samples. DNA was extracted from the specimens and investigated for the presence of HSV-1 by nested polymerase chain reaction (NPCR) and DNA sequencing.

RESULTS: HSV-1 was detected in 14 (54%) of the healthy samples, in 19 (36%) of the oral leukoplakia samples, and in 14 (52%) of the OSCC samples. The differences were not statistically significant.

CONCLUSIONS: We observed a high incidence of HSV-1 in healthy oral mucosa, oral leukoplakia, and OSCC tissues. Thus, no connection between OSCC development and presence of HSV-1 was detected.

Emneord
HSV-1; nested PCR; PCR
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-265746 (URN)10.4317/medoral.20630 (DOI)000369435500004 ()26449432 (PubMedID)
Tilgjengelig fra: 2015-11-03 Laget: 2015-11-03 Sist oppdatert: 2017-12-01bibliografisk kontrollert
Sahebjamiee, M., Sand, L., Karimi, S., Biettolahi, J. M., Jabalameli, F. & Jalouli, J. (2015). Prevalence of human papillomavirus in oral lichen planus in an Iranian cohort. Journal of Oral and Maxillofacial Pathology, 19(2), 170-174
Åpne denne publikasjonen i ny fane eller vindu >>Prevalence of human papillomavirus in oral lichen planus in an Iranian cohort
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2015 (engelsk)Inngår i: Journal of Oral and Maxillofacial Pathology, ISSN 0973-029X, E-ISSN 1998-393X, Vol. 19, nr 2, s. 170-174Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Context: Oral lichen planus (OLP) is a chronic inflammatory mucocutaneous disease with female predominance with the potential for malignant transformation. Human papillomavirus (HPV) is associated with both malignant and benign disease in the head and neck region. Aims: The present study assesses the prevalence of high-risk HPV-16 and HPV-18 in tissue and saliva samples from an Iranian population diagnosed with OLP. Subjects and Methods: Deoxyribonucleic acid was extracted and investigated using polymerase chain reaction from tissue and saliva samples of the same individuals from 40 OLP cases and saliva samples of 40 healthy controls. Statistical Analysis: The prevalence of data was compared using the Chi-square test and inter-group differences were analyzed using Fisher's exact test. Results: OLP specimens were HPV-positive in 11 of 40 (27.5%) cases compared with three of 40 (7.5%) saliva specimens, representing a statistically significant difference (P = 0.0367). HPV-16 and HPV-18 were positive in eight of 40 (20%) OLP tissues and three of 40 (7.5%) saliva samples. Five of the 40 healthy saliva samples were positive for HPV-16. In the OLP patients with dysplasia, four of seven tissue samples were HPV-positive; threeof these four were HPV-16- positive in comparision with seven of 33 HPV-positive samples from OLP patients without dysplasia. Conclusions: Biopsies were more accurate than saliva analysis for evaluating HPV prevalence in OLP patients. HPV prevalence was higher in dysplastic than nondysplastic OLP lesions in this Iranian cohort.

HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-265747 (URN)DOI: 10.4103/0973-029X.164528 (DOI)
Tilgjengelig fra: 2015-11-03 Laget: 2015-11-03 Sist oppdatert: 2017-12-01
Sand, L., Jalouli, M., Jalouli, J., Ibrahim, S., Hasseus, B., Ohman, J. & Hirsch, J.-M. (2015). Virus and tobacco use in premalignant and malignant oral lesions. Paper presented at 20th World Congress on Advances in Oncology and 18th International Symposium on Molecular Medicine, October 8-10, 2015 Metropolitan Hotel, Athens, Greece. International Journal of Molecular Medicine, 36(Supplement: 1), S27-S27
Åpne denne publikasjonen i ny fane eller vindu >>Virus and tobacco use in premalignant and malignant oral lesions
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2015 (engelsk)Inngår i: International Journal of Molecular Medicine, ISSN 1107-3756, E-ISSN 1791-244X, Vol. 36, nr Supplement: 1, s. S27-S27Artikkel i tidsskrift, Meeting abstract (Annet vitenskapelig) Published
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-266289 (URN)000361863000091 ()
Konferanse
20th World Congress on Advances in Oncology and 18th International Symposium on Molecular Medicine, October 8-10, 2015 Metropolitan Hotel, Athens, Greece
Tilgjengelig fra: 2015-11-06 Laget: 2015-11-06 Sist oppdatert: 2017-12-01bibliografisk kontrollert
Ramadhan, A., Gavelin, P., Hirsch, J. M. & Sand, L. (2014). A retrospective study of patients with mandibular fractures treated at a Swedish University Hospital 1999-2008. Annals of maxillofacial surgery, 4(2), 178-181
Åpne denne publikasjonen i ny fane eller vindu >>A retrospective study of patients with mandibular fractures treated at a Swedish University Hospital 1999-2008
2014 (engelsk)Inngår i: Annals of maxillofacial surgery, ISSN 2231-0746, Vol. 4, nr 2, s. 178-181Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

BACKGROUND:

Fracture of the mandible is one of the most common fractures of the maxillofacial skeleton. However, the etiology, gender, and age distribution vary between different regions and countries, and the purpose of this study was to evaluate the current trend of patients with mandibular fractures at the Department of Oral and Maxillofacial Surgery, in Uppsala, Sweden.

AIM:

The aim of this retrospective study was to analyze patients with mandibular fractures treated in the University Hospital of Uppsala (the county capital of Uppland) Sweden during a 10-year period (1999-2008).

MATERIALS AND METHODS:

This study was designed as a retrospective study of patients admitted to the Department of Oral and Maxillofacial Surgery at Uppsala University Hospital, Sweden. The location of fractures was evaluated clinically by the surgeon and on the X-rays. We classified the data according to gender, age, etiology, day of the week, month of the year, fracture site, and method of treatment of the fractures.

RESULTS:

Records were collected from patient charts from 266 patients. One hundred eighty-seven patients (70%) with mandibular fractures were of male gender, and 132 patients (50%) were aged 16-30 years. Interpersonal violence constituted the most common etiological factor for mandibular fractures (24%), followed by falls (23%). Forty-nine percentages of the patients were treated surgically, and 51% were treated conservatively. There was an increase of the annual incidence of fractures toward the end of the period, even though not statistically significant.

CONCLUSIONS:

Mandibular fractures occurred primarily among younger men between 16- and 30-year-old. Condyle fractures were the most common fracture site and 50% of the patients required surgery. Summer months and weekends were the most common time of mandibular fractures.

HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-248227 (URN)10.4103/2231-0746.147119 (DOI)25593868 (PubMedID)
Tilgjengelig fra: 2015-03-31 Laget: 2015-03-30 Sist oppdatert: 2015-03-31bibliografisk kontrollert
Sand, L., Wallström, M. & Hirsch, J.-M. (2014). Smokeless tobacco, viruses and oral cancer. Oral health and dental management, 13(2), 372-378
Åpne denne publikasjonen i ny fane eller vindu >>Smokeless tobacco, viruses and oral cancer
2014 (engelsk)Inngår i: Oral health and dental management, ISSN 2247-2452, Vol. 13, nr 2, s. 372-378Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Oral Squamous Cell Carcinoma (OSCC) is the most common epithelial malignancy in the oral cavity. OSCCs and their variants constitute over 90% of oral malignancies, and the disease is associated with poor prognosis. OSCC is a complex malignancy where environmental factors, viral infections, and genetic alterations most likely interact, and thus give rise to the malignant condition. The International Agency for Research on Cancer (IARC) in 2007 concluded: "there is sufficient evidence in humans to establish smokeless tobacco as carcinogenic, i.e. smokeless tobacco causes cancer of the oral cavity and pancreas". ST products contain a large array of carcinogens, although the number found is actually smaller than in cigarette smoke. Worldwide, ST products have many different names depending on the region where it is produced. However, there are two main types of ST, chewing tobacco and snuff. It is estimated that approximately 150 million people in the world use ST. Herein, we review available literature regarding smokeless tobacco and oral Carcinogenesis. We also discuss the role of viral infections in combination with ST in OSCC development.

HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-230654 (URN)24984650 (PubMedID)
Tilgjengelig fra: 2014-08-27 Laget: 2014-08-27 Sist oppdatert: 2014-09-04bibliografisk kontrollert
Sand, L. & Jalouli, J. (2014). Viruses and oral cancer. Is there a link?. Microbes and infection, 16(5), 371-378
Åpne denne publikasjonen i ny fane eller vindu >>Viruses and oral cancer. Is there a link?
2014 (engelsk)Inngår i: Microbes and infection, ISSN 1286-4579, E-ISSN 1769-714X, Vol. 16, nr 5, s. 371-378Artikkel, forskningsoversikt (Fagfellevurdert) Published
Abstract [en]

Oral squamous cell carcinoma (OSCC) is the most common malignant tumour of the oral cavity. The aetiology of epithelial cancer of the head and neck is considered to be a multifactorial, sequential process. DNA viruses are found in many different cancers and are also capable of transforming cells to a malignant phenotype. Human Papilloma Virus (HPV) has been proposed as risk factors in OSCC development and HPV type 16 is the most important subtype. Other oncogenic virus species i.e., Epstein-Barr Virus and Herpes Simplex Virus Type 1 have been proposed to be involved in oral carcinogenesis. However, no convincing evidence exist that they are an established risk factor in OSCC. Therefore more studies are needed in order to clarify the different aspects of virus involvement. Here, we review the existing literature on viral involvement in oral cancer.

HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-231576 (URN)10.1016/j.micinf.2014.02.009 (DOI)000347866500002 ()24613199 (PubMedID)
Tilgjengelig fra: 2014-09-09 Laget: 2014-09-09 Sist oppdatert: 2017-12-05bibliografisk kontrollert
Jawert, F., Hasseus, B., Kjeller, G., Magnusson, B., Sand, L. & Larsson, L. (2013). Loss of 5-Hydroxymethylcytosine and TET2 in Oral Squamous Cell Carcinoma. Anticancer Research, 33(10), 4325-4328
Åpne denne publikasjonen i ny fane eller vindu >>Loss of 5-Hydroxymethylcytosine and TET2 in Oral Squamous Cell Carcinoma
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2013 (engelsk)Inngår i: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 33, nr 10, s. 4325-4328Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Aim: Epigenetic modifications, such as DNA methylation, are considered important in the regulation of target genes in cancer development. 5-Hydroxymethylcytosine (5hmC) was recently discovered to be related to the process of malignant transformation. The influence of DNA methylation in oral squamous cell carcinomas (OSCC) is not fully- understood. Therefore, the aim of the present study was to investigate the DNA methylation pattern in OSCC compared to healthy oral epithelium. Materials and Methods: Oral mucosal samples from patients with OSCC (n=15) and healthy mucosa (n=12) were analyzed using immunohistochemistry with antibodies against 5hmC, 5mC and ten-eleven-translocation-2 (TET2). Results: A significant decrease in 5hmC and TET2 expression was found in OSCC compared to healthy oral epithelium. In contrast, there was a significant increase in 5mC expression in OSCC compared to healthy epithelium. Conclusion: Our results indicate that loss of 5hmC is an epigenetic event of OSCC.

HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-211893 (URN)000326204400023 ()24122999 (PubMedID)
Tilgjengelig fra: 2013-12-03 Laget: 2013-12-03 Sist oppdatert: 2017-12-06bibliografisk kontrollert
Jalouli, J., Jalouli, M. M., Sapkota, D., Ibrahim, S. O., Larsson, P.-A. & Sand, L. (2012). Human Papilloma Virus, Herpes Simplex Virus and Epstein Barr Virus in Oral Squamous Cell Carcinoma from Eight Different Countries. Anticancer Research, 32(2), 571-580
Åpne denne publikasjonen i ny fane eller vindu >>Human Papilloma Virus, Herpes Simplex Virus and Epstein Barr Virus in Oral Squamous Cell Carcinoma from Eight Different Countries
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2012 (engelsk)Inngår i: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 32, nr 2, s. 571-580Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Oral squamous cell carcinoma (OSCC) is a major health problem in many parts of the world, and the major causative agents are thought to he the use of alcohol and tobacco. Oncogenic viruses have also been suggested to be involved in OSCC development. This study investigated the prevalence of human papillomaviruses (HPV), herpes simplex virus (HSV) and Epstein-Barr virus (EBV) in 155 OSCC from eight different countries from different ethnic groups, continents and with different socioeconomic backgrounds. 41 A total of OSCCs were diagnosed in the tongue (26%) and 23 in the floor of the mouth (15%); the other 91 OSCCs were diagnosed in other locations (59%). The patients were also investigated regarding the use of alcohol and smoking and smokeless tobacco habits. Tissue samples were obtained from formalin-fixed, paraffin-embedded samples of the OSCC. DNA was extracted and the viral genome was examined by single, nested and seminested PCR assays. Sequencing of double-stranded DNA from the PCR product was carried out. Following sequencing of the HPV-, HSV- and EBV-positive PCR products, 100% homology between the sampels was found. Of all the 155 OSCCs examined, 85 (55%) were positive for EBV, 54 (35%) for HPV and 24 (15%) for HSV. The highest prevalence of HPV was seen in Sudan (65%), while HSV (55%) and EBV (80%) were most prevalent in the UK. In 34% (52/155) of all the samples examined, co-infection by two (46/155=30%) or three (6/155=4%) virus specimens was detected. The most frequent double infection was HPV with EBV in 21% (32/155) of all OSCCs. There was a statistically significant higher proportion of samples with HSV (p=0.026) and EBV (p=0.015) in industrialized countries (Sweden, Norway, UK and USA) as compared to developing countries (Sudan, India, Sri Lanka and Yemen). Furthermore, there was a statistically significant higher co-infection of HSV and EBV in samples from industrialized countries (p=0.00031). No firm conclusions could be drawn regarding the relationship between alcohol, tobacco and virus infections. The significance of our findings must be put in relation to other risk factors and these observations warrant further studies to determine the possible role of viral infections and co-infections with HPV, EBV and HSV as risk markers for the development of OSCC.

Emneord
Epstein Barr virus, herpes simplex virus, human papilloma virus, oral squamous cell carcinoma
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-169954 (URN)000299985800023 ()22287747 (PubMedID)
Tilgjengelig fra: 2012-03-08 Laget: 2012-03-07 Sist oppdatert: 2017-12-07bibliografisk kontrollert
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