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Manivel, Vivek AnandORCID iD iconorcid.org/0000-0002-6746-7372
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Fromell, K., Adler, A., Åman, A., Manivel, V. A., Huang, S., Dührkop, C., . . . Nilsson, B. (2020). Assessment of the Role of C3(H2O) in the Alternative Pathway. Frontiers in Immunology, 11, Article ID 530.
Åpne denne publikasjonen i ny fane eller vindu >>Assessment of the Role of C3(H2O) in the Alternative Pathway
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2020 (engelsk)Inngår i: Frontiers in Immunology, ISSN 1664-3224, E-ISSN 1664-3224, Vol. 11, artikkel-id 530Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

In this study we investigate the hydrolysis of C3 to C3(H2O) and its ability to initiate activation via the alternative pathway (AP) of the complement system. The internal thioester bond within C3 is hydrolyzed by water in plasma because of its inherent lability. This results in the formation of non-proteolytically activated C3(H2O) which is believed have C3b-like properties and be able to form an active initial fluid phase C3 convertase together with Factor B (FB). The generation of C3(H2O) occurs at a low but constant rate in blood, but the formation can be greatly accelerated by the interaction with various surfaces or nucleophilic and chaotropic agents. In order to more specifically elucidate the relevance of the C3(H2O) for AP activation, formation was induced in solution by repeated freeze/thawing, methylamine or KCSN treatment and named C3(x) where the x can be any of the reactive nucleophilic or chaotropic agents. Isolation and characterization of C3(x) showed that it exists in several forms with varying attributes, where some have more C3b-like properties and can be cleaved by Factor I in the presence of Factor H. However, in common for all these variants is that they are less active partners in initial formation of the AP convertase compared with the corresponding activity of C3b. These observations support the idea that formation of C3(x) in the fluid phase is not a strong initiator of the AP. It is rather likely that the AP mainly acts as an amplification mechanism of complement activation that is triggered by deposition of target-bound C3b molecules generated by other means.

sted, utgiver, år, opplag, sider
FRONTIERS MEDIA SA, 2020
Emneord
complement, C3, C3(H2O), C3b, alternative pathway, C3 convertase
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-410958 (URN)10.3389/fimmu.2020.00530 (DOI)000526750400001 ()32296436 (PubMedID)
Forskningsfinansiär
Swedish Research Council, 2016-2075-5.1Swedish Research Council, 2016-04519
Tilgjengelig fra: 2020-05-28 Laget: 2020-05-28 Sist oppdatert: 2020-05-28bibliografisk kontrollert
Nilsson Ekdahl, K., Mohlin, C., Adler, A., Åman, A., Manivel, V. A., Sandholm, K., . . . Nilsson, B. (2019). Is generation of C3(H2O) necessary for activation of the alternative pathway in real life?. Paper presented at 17th European Meeting on Complement in Human Disease (EMCHD), 14-17 September 2019, Madrid, SPAIN. Molecular Immunology, 114, 353-361
Åpne denne publikasjonen i ny fane eller vindu >>Is generation of C3(H2O) necessary for activation of the alternative pathway in real life?
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2019 (engelsk)Inngår i: Molecular Immunology, ISSN 0161-5890, E-ISSN 1872-9142, Vol. 114, s. 353-361Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

In the alternative pathway (AP) an amplification loop is formed, which is strictly controlled by various fluid-phase and cell-bound regulators resulting in a state of homeostasis. Generation of the “C3b-like” C3(H2O) has been described as essential for AP activation, since it conveniently explains how the initial fluid-phase AP convertase of the amplification loop is generated. Also, the AP has a status of being an unspecific pathway despite thorough regulation at different surfaces.

During complement attack in pathological conditions and inflammation, large amounts of C3b are formed by the classical/lectin pathway (CP/LP) convertases. After the discovery of LP's recognition molecules and its tight interaction with the AP, it is increasingly likely that the AP acts in vivo mainly as a powerful amplification mechanism of complement activation that is triggered by previously generated C3b molecules initiated by the binding of specific recognition molecules.

Also in many pathological conditions caused by a dysregulated AP amplification loop such as paroxysmal nocturnal hemoglobulinuria (PNH) and atypical hemolytic uremic syndrome (aHUS), C3b is available due to minute LP and CP activation and/or generated by non-complement proteases. Therefore, C3(H2O) generation in vivo may be less important for AP activation during specific attack or dysregulated homeostasis, but may be an important ligand for C3 receptors in cell-cell interactions and a source of C3 for the intracellular complement reservoir.

Emneord
Complement system, C3(H2O), Conformation, Analysis, Proteases, Alternative pathway
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-396739 (URN)10.1016/j.molimm.2019.07.032 (DOI)000490625600041 ()31446306 (PubMedID)
Konferanse
17th European Meeting on Complement in Human Disease (EMCHD), 14-17 September 2019, Madrid, SPAIN
Forskningsfinansiär
Swedish Research Council, 2016-2075-5.1Swedish Research Council, 2016-04519German Research Foundation (DFG), CRC1149A01
Tilgjengelig fra: 2019-11-20 Laget: 2019-11-20 Sist oppdatert: 2019-12-14bibliografisk kontrollert
Manivel, V. A., van der Woude, D., Toes, R., Filer, A., Raza, K. & Rönnelid, J. (2018). Antibodies against collagen type II are not a general marker of acute arthritis onset [Letter to the editor]. Annals of the Rheumatic Diseases, 77(6), 954-956
Åpne denne publikasjonen i ny fane eller vindu >>Antibodies against collagen type II are not a general marker of acute arthritis onset
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2018 (engelsk)Inngår i: Annals of the Rheumatic Diseases, ISSN 0003-4967, E-ISSN 1468-2060, Vol. 77, nr 6, s. 954-956Artikkel i tidsskrift, Letter (Annet vitenskapelig) Published
Emneord
autoantibodies, disease activity, early rheumatoid arthritis, gout, reactive arthritis
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-343356 (URN)10.1136/annrheumdis-2017-211974 (DOI)000433228800036 ()28839108 (PubMedID)
Tilgjengelig fra: 2018-02-26 Laget: 2018-02-26 Sist oppdatert: 2018-12-10bibliografisk kontrollert
Too, C. L., Manivel, V. A., Persinidou, E., Rönnelid, J. & Murad, S. (2018). ANTI-COLLAGEN TYPE II ANTIBODIES ARE ASSOCIATED WITH EARLY INFLAMMATION IN MALAYSIAN RHEUMATOID ARTHRITIS PATIENTS WITH THREE DIFFERENT ETHINICITIES. Paper presented at 38th European Workshop on Rheumatology Research, FEB 22-24, 2018, Geneva, SWITZERLAND. Annals of the Rheumatic Diseases, 77, A32-A33
Åpne denne publikasjonen i ny fane eller vindu >>ANTI-COLLAGEN TYPE II ANTIBODIES ARE ASSOCIATED WITH EARLY INFLAMMATION IN MALAYSIAN RHEUMATOID ARTHRITIS PATIENTS WITH THREE DIFFERENT ETHINICITIES
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2018 (engelsk)Inngår i: Annals of the Rheumatic Diseases, ISSN 0003-4967, E-ISSN 1468-2060, Vol. 77, s. A32-A33Artikkel i tidsskrift, Meeting abstract (Annet vitenskapelig) Published
sted, utgiver, år, opplag, sider
BMJ PUBLISHING GROUP, 2018
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-357184 (URN)000429442700068 ()
Konferanse
38th European Workshop on Rheumatology Research, FEB 22-24, 2018, Geneva, SWITZERLAND
Tilgjengelig fra: 2018-08-14 Laget: 2018-08-14 Sist oppdatert: 2018-08-14bibliografisk kontrollert
Manivel, V. A., Mullazehi, M., Padyukov, L., Westerlind, H., Klareskog, L., Alfredsson, L., . . . Rönnelid, J. (2017). Anti-Collagen Type Ii Antibodies Are Associated With An Acute Onset Rheumatoid Arthritis Phenotype And Prognosticate Lower Degree Of Inflammation. Paper presented at Annual European Congress of Rheumatology, JUN 14-17, 2017, Madrid, SPAIN. Annals of the Rheumatic Diseases, 76, 228-228
Åpne denne publikasjonen i ny fane eller vindu >>Anti-Collagen Type Ii Antibodies Are Associated With An Acute Onset Rheumatoid Arthritis Phenotype And Prognosticate Lower Degree Of Inflammation
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2017 (engelsk)Inngår i: Annals of the Rheumatic Diseases, ISSN 0003-4967, E-ISSN 1468-2060, Vol. 76, s. 228-228Artikkel i tidsskrift, Meeting abstract (Annet vitenskapelig) Published
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-346626 (URN)10.1136/annrheumdis-2017-eular.2776 (DOI)000413181400613 ()
Konferanse
Annual European Congress of Rheumatology, JUN 14-17, 2017, Madrid, SPAIN
Tilgjengelig fra: 2018-03-21 Laget: 2018-03-21 Sist oppdatert: 2018-03-21bibliografisk kontrollert
Manivel, V. A., Mullazehi, M., Padyukov, L., Westerlind, H., Klareskog, L., Alfredson, L., . . . Rönnelid, J. (2017). Anticollagen type II antibodies are associated with an acute onset rheumatoid arthritis phenotype and prognosticate lower degree of inflammation during 5 years follow-up. Annals of the Rheumatic Diseases, 76(9), 1529-1536
Åpne denne publikasjonen i ny fane eller vindu >>Anticollagen type II antibodies are associated with an acute onset rheumatoid arthritis phenotype and prognosticate lower degree of inflammation during 5 years follow-up
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2017 (engelsk)Inngår i: Annals of the Rheumatic Diseases, ISSN 0003-4967, E-ISSN 1468-2060, Vol. 76, nr 9, s. 1529-1536Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Objective

Antifibrillar collagen type II (anti-CII) antibody-positive patients with rheumatoid arthritis (RA) have early but not late signs of increased inflammation and joint erosions. We wanted to replicate this in a large RA cohort, and to relate to human leukocyte antigen (HLA)-DRB1* alleles.

Methods

Anti-CII and anti-cyclic citrullinated peptide (CCP)2 were measured at baseline in 773 patients with RA from the Swedish Epidemiological Investigation in Rheumatoid Arthritis (EIRA) study with clinical follow-up data from the Swedish Rheumatology Quality Register (SRQ) registry, and 1476 with HLA-DRB1* information. Comparisons were done concerning C reactive protein (CRP), erythrocyte sedimentation rate (ESR), tender joint count (TJC), swollen joint count (SJC), Disease Activity Score encompassing 28 joints based on ESR (DAS28), DAS28CRP, pain-Visual Analogue Scale (VAS), global-VAS and Health Assessment Questionnaire Score (HAQ) at eight occasions during 5 years, and association with HLA-DRB1* alleles.

Results

Anti-CII associated with elevated CRP, ESR, SJC, DAS28 and DAS28CRP at diagnosis and up to 6 months, whereas anti-CCP2 associated with SJC and DAS28 from 6 months to 5 years, but not earlier. The anti-CII-associated phenotype was strong, and predominated in anti-CII/anti-CCP2 double-positive patients. Anti-CII was associated with improvements in CRP, ESR, SJC, TJC and DAS28, whereas anti-CCP2 was associated with deteriorations in SJC and DAS28 over time. Anti-CII-positive patients achieved European League Against Rheumatism good or moderate response more often than negative patients. Anti-CII was positively associated with HLA-DRB1*01 and HLA-DRB1*03, with significant interaction, and double-positive individuals had >14 times higher mean anti-CII levels than HLA double negatives. Whereas smoking was associated with elevated anti-CCP2 levels, smokers had lower anti-CII levels.

Conclusions

Anti-CII seropositive RA represents a distinct phenotype, in many respects representing the converse to the clinical, genetic and smoking associations described for anticitrullinated protein peptide autoantibodies. Although not diagnostically useful, early anti-CII determinations predict favourable inflammatory outcome in RA.

HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-311956 (URN)10.1136/annrheumdis-2016-210873 (DOI)000407833100022 ()28336519 (PubMedID)
Tilgjengelig fra: 2017-01-03 Laget: 2017-01-03 Sist oppdatert: 2018-02-27bibliografisk kontrollert
Weitoft, T., Larsson, A., Saxne, T., Manivel, V. A., Lysholm, J., Knight, A. & Rönnelid, J. (2017). Pentraxin 3 in serum and synovial fluid of patients with rheumatoid arthritis with and without autoantibodies. Scandinavian Journal of Rheumatology, 46(5), 346-352
Åpne denne publikasjonen i ny fane eller vindu >>Pentraxin 3 in serum and synovial fluid of patients with rheumatoid arthritis with and without autoantibodies
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2017 (engelsk)Inngår i: Scandinavian Journal of Rheumatology, ISSN 0300-9742, E-ISSN 1502-7732, Vol. 46, nr 5, s. 346-352Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

OBJECTIVES:

Pentraxin 3 (PTX3) is a locally produced multifunctional protein involved in inflammation, matrix deposition, and immunity. As patients with seropositive rheumatoid arthritis (RA) have a more severe disease course and higher risk of joint destruction than seronegative patients, the aim of the present study was to examine differences in PTX3 in synovial fluid (SF) (and serum) in seropositive compared to seronegative RA, and other local markers of inflammation and destruction.

METHOD:

Ninety-seven RA patients with knee effusion were included. Serum and SF levels of PTX3, as well as serum levels of anti-citrullinated protein antibody and rheumatoid factor of immunoglobulin A and M subclasses, and markers of inflammation and potential destruction in SF: white blood cell counts, tumour necrosis factor, interleukin-6, vascular endothelial growth factor, metalloproteinase 3, and cartilage oligomeric matrix protein, were analysed. In addition, a radiographic knee examination was performed.

RESULTS:

Seropositive patients had significantly higher PTX3 levels in SF than seronegative patients, whereas there was no difference for serum levels. SF-PTX3 levels correlated with disease activity and with local inflammatory markers, especially polymorphonuclear cells, and with autoantibody levels. There was no correlation between PTX3 levels in serum and SF.

CONCLUSION:

The correlation of disease activity and autoantibody levels with SF-PTX3 levels in antibody-positive patients suggests a role for PTX3 in the inflammatory process specifically in seropositive RA joints, and supports the hypothesis that seropositive and seronegative RA are different disease entities. Polymorphonuclear granulocytes may be an important source of PTX3 in RA SF.

HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-310944 (URN)10.1080/03009742.2016.1244288 (DOI)000411129500002 ()27973973 (PubMedID)
Tilgjengelig fra: 2016-12-20 Laget: 2016-12-20 Sist oppdatert: 2018-02-27bibliografisk kontrollert
Manivel, V. A. (2017). The role of anti-collagen type II antibodies in the pathogenesis and prognosis of rheumatoid arthritis. (Doctoral dissertation). Uppsala: Acta Universitatis Upsaliensis
Åpne denne publikasjonen i ny fane eller vindu >>The role of anti-collagen type II antibodies in the pathogenesis and prognosis of rheumatoid arthritis
2017 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Rheumatoid arthritis (RA) which affects 0.5-1% of the world population and is characterised by joint erosions and presence of the autoantibodies anti-citrullinated protein antibodies (ACPA) and rheumatoid factor. Collagen II (CII) is a joint-specific antigen and we have shown that antibodies against CII (anti-CII) are present in around 8% of RA patients. RA patients with anti-CII are characterized by acute RA onset with elevated CRP and early joint erosions at the time of RA onset. Polymorphonuclear granulocytes (PMN) and peripheral blood mononuclear cells (PBMC) are abundant in RA synovial fluids, where they can interact with anti-CII, thus forming immune complexes (IC) with CII. In my thesis I have shown that PMN upregulated the cell surface markers CD66b and CD11b and downregulated CD16 and CD32 after stimulation with anti-CII IC. These changes in CD66b and CD16 associated to joint erosions to a larger extent than did PBMC responses to anti-CII IC. PMN cocultured with PBMC and stimulated with anti-CII IC showed augmented chemokine production that was dependent on TLR4 and functionally active PMN enzymes. This mechanism can lead to accumulation of inflammatory cells in joints of RA patients who are anti-CII positive around the time of RA diagnosis, and may thus help explain the acute onset RA phenotype associated with anti-CII.

In a large Swedish RA cohort, anti-CII associated with elevations in clinical and laboratory measures of disease activity at diagnosis and until 6 months, whereas ACPA associated with late inflammation. Anti-CII seropositive RA was associated with improvements in clinical measurements and was negatively associated with smoking in contrast to ACPA that was associated with worseneing of clinical symptoms and associated positively with smoking. Anti-CII levels associated to  HLADRB1*03 and  HLADRB1*01 whereas ACPA showed negative association to HLA-DRB1*03. In a Malaysian RA cohort anti-CII also associated to elevated CRP at the time of diagnosis.

Anti-CII seropositive RA represents a distinct phenotype, in many respects representing the converse  to the clinical, genetic and smoking associations described for ACPA. Early determinations of anti-CII in parallel to ACPA predict the inflammatory outcome in RA.

sted, utgiver, år, opplag, sider
Uppsala: Acta Universitatis Upsaliensis, 2017. s. 62
Serie
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 1289
Emneord
Rheumatoid arthritis; Anti-collagen type II antibodies; HLADRB1*; Granulocytes; prognosis;
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-311959 (URN)978-91-554-9792-7 (ISBN)
Disputas
2017-02-28, Rudbecksalen, Daghammarskjölds väg 20, Uppsala, 09:00 (engelsk)
Opponent
Veileder
Forskningsfinansiär
Swedish Research Council
Tilgjengelig fra: 2017-02-06 Laget: 2017-01-03 Sist oppdatert: 2018-02-27
Manivel, V. A., Sohrabian, A. & Rönnelid, J. (2016). Granulocyte-augmented chemokine production induced by type II collagen containing immune complexes is mediated via TLR4 in rheumatoid arthritis patients. European Journal of Immunology, 46(12), 2822-2834
Åpne denne publikasjonen i ny fane eller vindu >>Granulocyte-augmented chemokine production induced by type II collagen containing immune complexes is mediated via TLR4 in rheumatoid arthritis patients
2016 (engelsk)Inngår i: European Journal of Immunology, ISSN 0014-2980, E-ISSN 1521-4141, Vol. 46, nr 12, s. 2822-2834Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Rheumatoid arthritis (RA) patients with early elevations of antibodies against collagen type II (CII) have a distinct acute onset phenotype, associated with cytokine induction by surface-bound anti-CII-containing immune complexes (ICs) and high C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR). Polymorphonuclear granulocytes (PMNs) and peripheral blood mononuclear cells (PBMCs) are abundant in the vicinity of CII in RA joints, and both PMN and PBMC reactivity against anti-CII IC individually relate to early joint destruction and early elevation of CRP and ESR in RA. We searched for CII-dependent mechanisms that might attract PMNs and PBMCs to RA joints. Human PBMCs and PMNs were stimulated with anti-CII ICs and control ICs, either individually or in cocultures. Cocultured PMNs and PBMCs stimulated with anti-CII ICs synergistically augmented production of the chemokines CXCL8, RANTES and MCP-1, whereas downregulation was seen with control IC. This upregulation was unique to chemokines, as TNF-α, IL-1β, and GM-CSF were downregulated in anti-CII IC-stimulated cocultures. The coculture-associated chemokine upregulation depended on endogenous TLR4 ligand(s) and functionally active PMN enzymes, and was partially mediated by GM-CSF. As anti-CII levels peak around the time of RA diagnosis, this mechanism can attract inflammatory cells to joints in early RA and intensify the anti-CII-associated acute onset RA phenotype.

sted, utgiver, år, opplag, sider
Wiley-VCH Verlagsgesellschaft, 2016
Emneord
Anticollagen antibodies, Chemokine, Collagen type II, Immune complexes, Polymorphonuclear granulocytes, Rheumatoid arthritis, TLR4
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-308558 (URN)10.1002/eji.201646496 (DOI)000392940400021 ()27621106 (PubMedID)
Forskningsfinansiär
Swedish Research CouncilSwedish Rheumatism Association
Tilgjengelig fra: 2016-11-28 Laget: 2016-11-28 Sist oppdatert: 2018-02-27bibliografisk kontrollert
Manivel, V. A., Sohrabian, A., Wick, M. C., Mullazehi, M., Håkansson, L. D. & Rönnelid, J. (2015). Anti-type II collagen immune complex-induced granulocyte reactivity is associated with joint erosions in RA patients with anti-collagen antibodies. Arthritis Research & Therapy, 17, Article ID 8.
Åpne denne publikasjonen i ny fane eller vindu >>Anti-type II collagen immune complex-induced granulocyte reactivity is associated with joint erosions in RA patients with anti-collagen antibodies
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2015 (engelsk)Inngår i: Arthritis Research & Therapy, ISSN 1478-6354, E-ISSN 1478-6362, Vol. 17, artikkel-id 8Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Introduction: Rheumatoid arthritis (RA) patients with autoantibodies against collagen type II (CII) are characterized by acute RA onset with elevated inflammatory measures and early joint erosions as well as increased production of tumor necrosis factor-alpha (TNF-alpha) by peripheral blood mononuclear cells (PBMC) stimulated by anti-CII immune complexes (IC) in vitro. Polymorphonuclear granulocytes (PMN) are abundant in RA synovial fluids, where they might interact directly with anti-CII IC in the articular cartilage, but no studies have investigated PMN responses towards anti-CII IC. The aim was to investigate whether PMN react towards anti-CII IC, and to what extent such reactivity might relate to the clinical acute onset RA phenotype associated with elevated levels of anti-CII. Methods: PMN and PBMC isolated from healthy donors were stimulated with IC made with a set of 72 baseline patient sera (24 anti-CII positive, 48 anti-CII negative) chosen from a clinically well-characterized RA cohort with two-year radiological follow-up with Larsen scoring. PMN expression of cluster of differentiation (CD) 11b, CD66b, CD16 and CD32 was measured by flow cytometry, whereas PMN production of myeloperoxidase (MPO) and interleukin (IL)-17, and PBMC production of TNF-alpha was measured with enzyme linked immunosorbent assay. Results: PMN expression of CD11b, CD66b and MPO, and PBMC production of TNF-alpha were upregulated whereas PMN expression of CD16 and CD32 were downregulated by anti-CII IC. CD16, CD66b, and MPO production correlated to serum anti-CII levels (Spearman's rho = 0.315, 0.675 and 0.253, respectively). CD16 was associated with early joint erosions (P = 0.024, 0.034, 0.046 at baseline, one and two years) and CD66b was associated with changes in joint erosions (P = 0.017 and 0.016, at one and two years compared to baseline, respectively). CD66b was associated with baseline C-reactive protein and PBMC production of TNF-alpha was associated with baseline erythrocyte sedimentation rate, in accordance with our earlier findings. No clinical associations were observed for MPO or IL-17. Conclusion: PMN responses against anti-CII IC are more closely associated with early joint erosions than are PBMC cytokine responses. PMN reactivity against anti-CII IC may contribute to joint destruction in newly diagnosed RA patients with high levels of anti-CII.

HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-251853 (URN)10.1186/s13075-015-0523-7 (DOI)000351573200001 ()25598326 (PubMedID)
Tilgjengelig fra: 2015-04-28 Laget: 2015-04-24 Sist oppdatert: 2018-02-27bibliografisk kontrollert
Organisasjoner
Identifikatorer
ORCID-id: ORCID iD iconorcid.org/0000-0002-6746-7372