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Fahlman, A., Lindsjo, J., Bergvall, U. A., Agren, E. O., Arvén Norling, T., Stridsberg, M., . . . Hoglund, O. (2021). Measurement of catestatin and vasostatin in wild boar Sus scrofa captured in a corral trap. BMC Research Notes, 14(1), Article ID 337.
Åpne denne publikasjonen i ny fane eller vindu >>Measurement of catestatin and vasostatin in wild boar Sus scrofa captured in a corral trap
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2021 (engelsk)Inngår i: BMC Research Notes, E-ISSN 1756-0500, Vol. 14, nr 1, artikkel-id 337Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Objective Our aim was to analyse the chromogranin A-derived peptides vasostatin and catestatin in serum from wild boar (Sus scrofa) captured in a corral trap. Acute capture-related stress quickly leads to a release of adrenalin and noradrenalin, but these hormones have a short half-life in blood and are difficult to measure. Chromogranin A (CgA), a glycoprotein which is co-released with noradrenalin and adrenalin, is relatively stable in circulation and the CgA-derived peptides catestatin and vasostatin have been measured in domestic species, but not yet in wildlife. Results Vasostatin and catestatin could be measured and the median (range) serum concentrations were 0.91 (0.54-2.86) and 0.65 (0.35-2.62) nmol/L, respectively. We conclude that the CgA-derived peptides vasostatin and catestatin can be measured in wild boar serum and may thus be useful as biomarkers of psychophysical stress.

sted, utgiver, år, opplag, sider
BioMed Central (BMC)Springer Nature, 2021
Emneord
Animal welfare, Catestatin, CgA, Live-trap capture, Stress, Trapping, Vasostatin, 3Rs
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-456323 (URN)10.1186/s13104-021-05742-1 (DOI)000692408400007 ()34461992 (PubMedID)
Tilgjengelig fra: 2021-10-18 Laget: 2021-10-18 Sist oppdatert: 2024-01-17bibliografisk kontrollert
Myhre, P. L., Ottesen, A. H., Faaren, A. L., Tveit, S. H., Springett, J., Pyylampi, J., . . . Røsjø, H. (2021). Performance of a Novel Research-Use-Only Secretoneurin ELISA in Patients with Suspected Acute Coronary Syndrome: Comparison with an Established Secretoneurin Radioimmunoassay. Cardiology, 146(5), 566-574
Åpne denne publikasjonen i ny fane eller vindu >>Performance of a Novel Research-Use-Only Secretoneurin ELISA in Patients with Suspected Acute Coronary Syndrome: Comparison with an Established Secretoneurin Radioimmunoassay
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2021 (engelsk)Inngår i: Cardiology, ISSN 0008-6312, E-ISSN 1421-9751, Vol. 146, nr 5, s. 566-574Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background: Circulating secretoneurin (SN) concentrations, as measured by established radioimmunoassay (RIA), risk stratify patients with cardiovascular disease. We now report data for a recently developed research-use-only SN enzyme-linked immunosorbent assay (ELISA) in patients with suspected acute coronary syndrome (ACS).

Methods: SN ELISA was developed according to industry standards and tested in 401 unselected chest pain patients. Blood samples were drawn <24 h from admission, and we adjudicated all hospitalizations as ACS or non-ACS. The mean follow-up was 6.2 years.

Results: SN ELISA with 2 monoclonal sheep anti-SN antibodies has a measuring range of 10–250 pmol/L and demonstrates excellent analytical precision and accuracy across the range of SN concentrations. SN measured by ELISA and RIA correlated in the chest pain patients: rho = 0.39, p < 0.001. SN concentrations were higher in ACS patients (n = 161 [40%]) than in non-ACS patients (n = 240) for both assays, with an area under the curve (AUC) of 0.66 (95% CI: 0.61–0.71) for ELISA and 0.59 (0.54–0.65) for RIA. SN concentrations were also higher in nonsurvivors (n = 65 [16%]) than survivors, with an AUC of 0.72 (0.65–0.79) for ELISA versus 0.64 (0.56–0.72) for RIA, p = 0.007, for difference between assays. Adjusting for age, sex, blood pressure, previous myocardial infarction, atrial fibrillation, and heart failure in multivariable analysis, SN concentrations as measured by ELISA, but not RIA, remained associated with mortality, with a hazard ratio of 1.71 (1.03–2.84), p = 0.038.

Conclusions: The novel SN ELISA has excellent performance, higher AUC for diagnosis, and superior prognostic accuracy compared to the established RIA in chest pain patients. 

sted, utgiver, år, opplag, sider
S. KargerS. Karger AG, 2021
Emneord
Secretoneurin, Enzyme-linked immunosorbent assay, Acute coronary syndrome
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-464092 (URN)10.1159/000517444 (DOI)000675444800001 ()34284402 (PubMedID)
Forskningsfinansiär
European Commission
Tilgjengelig fra: 2022-01-13 Laget: 2022-01-13 Sist oppdatert: 2024-01-15bibliografisk kontrollert
Hoglund, K., Haggstrom, J., Hoglund, O. V., Stridsberg, M., Tidholm, A. & Ljungvall, I. (2020). The chromogranin A-derived peptides catestatin and vasostatin in dogs with myxomatous mitral valve disease. Acta Veterinaria Scandinavica, 62(1), Article ID 43.
Åpne denne publikasjonen i ny fane eller vindu >>The chromogranin A-derived peptides catestatin and vasostatin in dogs with myxomatous mitral valve disease
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2020 (engelsk)Inngår i: Acta Veterinaria Scandinavica, ISSN 0044-605X, E-ISSN 1751-0147, Vol. 62, nr 1, artikkel-id 43Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background The protein chromogranin A (CgA) is stored and co-released with catecholamines from the stimulated adrenal glands. Increased plasma concentrations of CgA have been shown in people with heart disease. The aim of the study was to investigate whether plasma concentrations of the CgA-derived biologically active peptides catestatin and vasostatin were associated with the severity of myxomatous mitral valve disease (MMVD) in dogs and to assess potential associations between these blood variables and dog characteristics, echocardiographic variables, heart rate (HR), blood pressure (BP) and plasma N-terminal-proBNP (NT-proBNP) concentration. Sixty-seven privately owned dogs with or without MMVD were included. The dogs underwent physical examination, blood pressure measurement, blood sample collection, and echocardiographic examination. Plasma concentrations of catestatin and vasostatin were analyzed using radioimmunoassay. Results Catestatin concentration decreased with increasing left atrial and ventricular size (R-2 <= 0.09, P <= 0.019), and increased with increasing systolic and diastolic blood pressures (R-2 <= 0.08, P <= 0.038). Regression analyses showed no significant associations for vasostatin. No differences in plasma concentrations of catestatin or vasostatin were found between the disease severity groups used in the study. Conclusions In the present dog population, the catestatin concentration showed weak negative associations with left atrial and ventricular sizes, both of which are known to increase with increasing severity of MMVD. Furthermore, the catestatin concentration showed weak positive associations with blood pressure.

sted, utgiver, år, opplag, sider
BMC, 2020
Emneord
Canine, Catestatin, Heart, Sympathetic nervous system, Vasostatin
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-421051 (URN)10.1186/s13028-020-00541-3 (DOI)000560737100001 ()32758260 (PubMedID)
Tilgjengelig fra: 2020-10-06 Laget: 2020-10-06 Sist oppdatert: 2022-06-07bibliografisk kontrollert
Brynildsen, J., Petaja, L., Myhre, P. L., Lyngbakken, M. N., Nygard, S., Stridsberg, M., . . . Rosjo, H. (2019). Circulating Secretoneurin Concentrations After Cardiac Surgery: Data From the FINNish Acute Kidney Injury Heart Study. Critical Care Medicine, 47(5), E412-E419
Åpne denne publikasjonen i ny fane eller vindu >>Circulating Secretoneurin Concentrations After Cardiac Surgery: Data From the FINNish Acute Kidney Injury Heart Study
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2019 (engelsk)Inngår i: Critical Care Medicine, ISSN 0090-3493, E-ISSN 1530-0293, Vol. 47, nr 5, s. E412-E419Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Objectives:

Secretoneurin is associated with cardiomyocyte Ca2+ handling and improves risk prediction in patients with acute myocardial dysfunction. Whether secretoneurin improves risk assessment on top of established cardiac biomarkers and European System for Cardiac Operative Risk Evaluation II in patients undergoing cardiac surgery is not known.

Design:

Prospective, observational, single-center sub-study of a multicenter study.

Setting:

Prospective observational study of survival in patients undergoing cardiac surgery.

Patients:

A total of 619 patients undergoing cardiac surgery.

Interventions:

Patients underwent either isolated coronary artery bypass graft surgery, single noncoronary artery bypass graft surgery, two procedures, or three or more procedures. Procedures other than coronary artery bypass graft were valve surgery, surgery on thoracic aorta, and other cardiac surgery.

Measurements and Main Results:

We measured preoperative and postoperative secretoneurin concentrations and adjusted for European System for Cardiac Operative Risk Evaluation II, N-terminal pro-B-type natriuretic peptide, and cardiac troponin T concentrations in multivariate analyses. During 961 days of follow- up, 59 patients died (9.5%). Secretoneurin concentrations were higher among nonsurvivors compared with survivors, both before (168 pmol/L [quartile 1-3, 147-206 pmol/L] vs 160 pmol/L [131-193 pmol/L]; p = 0.039) and after cardiac surgery (173 pmol/L [129-217 pmol/L] vs 143 pmol/L [111-173 pmol/L]; p < 0.001). Secretoneurin concentrations decreased from preoperative to postoperative measurements in survivors, whereas we observed no significant decrease in secretoneurin concentrations among nonsurvivors. Secretoneurin concentrations were weakly correlated with established risk indices. Patients with the highest postoperative secretoneurin concentrations had worse outcome compared with patients with lower secretoneurin concentrations (p < 0.001 by the log-rank test) and postoperative secretoneurin concentrations were associated with time to death in multivariate Cox regression analysis: hazard ratio ln secretoneurin 2.96 (95% CI, 1.46-5.99; p = 0.003). Adding postoperative secretoneurin concentrations to European System for Cardiac Operative Risk Evaluation II improved patient risk stratification, as assessed by the integrated discrimination index: 0.023 (95% CI, 0.0043-0.041; p = 0.016).

Conclusions:

Circulating postoperative secretoneurin concentrations provide incremental prognostic information to established risk indices in patients undergoing cardiac surgery.

Emneord
biomarkers, cardiac surgery, coronary artery bypass graft, European System for Cardiac Operative Risk Evaluation II, risk assessment, secretoneurin
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-387601 (URN)10.1097/CCM.0000000000003670 (DOI)000469387900004 ()30730440 (PubMedID)
Tilgjengelig fra: 2019-06-26 Laget: 2019-06-26 Sist oppdatert: 2019-06-26bibliografisk kontrollert
Brynildsen, J., Myhre, P. L., Lyngbakken, M. N., Klaeboe, L. G., Stridsberg, M., Christensen, G., . . . Rosjo, H. (2019). Circulating secretoneurin concentrations in patients with moderate to severe aortic stenosis. Clinical Biochemistry, 71, 17-23
Åpne denne publikasjonen i ny fane eller vindu >>Circulating secretoneurin concentrations in patients with moderate to severe aortic stenosis
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2019 (engelsk)Inngår i: Clinical Biochemistry, ISSN 0009-9120, E-ISSN 1873-2933, Vol. 71, s. 17-23Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background: Secretoneurin (SN) concentrations provide important prognostic information in patients with myocardial dysfunction. Whether preoperative SN concentrations improve risk assessment in patients with moderate to severe aortic stenosis (AS) is unknown. Methods: We included 57 patients with moderate to severe AS referred for presurgical evaluation. All patients were examined with comprehensive echocardiography, electrocardiogram (ECG), and biochemical measurements and compared to 10 age- and sex-matched healthy subjects. Results: Median (quartile 1-3) SN concentrations were 141 (121-163) pmol/L in AS patients and 132 (106-148) pmol/L in control subjects (p = .17). Lower estimated creatinine clearance and use of diuretics, but not standard ECG or echocardiographic indices and cardiac biomarkers, were associated with increasing SN concentrations. Fifteen patients (26%) died during 3.5 years median follow-up. SN concentrations were higher in non-survivors than survivors: 156 (133-209) vs. 140 (116-155) pmol/L, p = .007. Higher SN concentrations were associated with increased risk of mortality also after adjustment for established risk indices, biomarkers, and status regarding valvular surgery: hazard ratio per lnSN 15.13 (95% CI 1.05-219.00); p = .046. Receiver operating characteristics area under the curve for SN to predict mortality was 0.74 (95% CI 0.60-0.88) compared to 0.73 (0.59-0.87) for high-sensitivity cardiac troponin T and 0.67 (0.51-0.82) for N-terminal pro-B-type natriuretic peptide. The previously identified cut-off of SN > 204 pmol/L in cardiac surgical patients predicted mortality also in this cohort. Conclusions: SN concentrations improve risk assessment in patients with moderate to severe AS by providing additional prognostic information to established risk indices such as echocardiography, ECG, and established cardiac biomarkers.

sted, utgiver, år, opplag, sider
PERGAMON-ELSEVIER SCIENCE LTD, 2019
Emneord
Aortic stenosis, Biomarkers, Secretoneurin, Cardiac surgery, Echocardiography
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-393647 (URN)10.1016/j.clinbiochem.2019.06.008 (DOI)000480675200003 ()31228433 (PubMedID)
Tilgjengelig fra: 2019-09-26 Laget: 2019-09-26 Sist oppdatert: 2019-09-26bibliografisk kontrollert
Shulman, R. J., Öhman, L., Stridsberg, M., Cain, K., Simren, M. & Heitkemper, M. (2019). Evidence of increased fecal granins in children with irritable bowel syndrome and correlates with symptoms. Neurogastroenterology and Motility, 31(1), Article ID e13486.
Åpne denne publikasjonen i ny fane eller vindu >>Evidence of increased fecal granins in children with irritable bowel syndrome and correlates with symptoms
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2019 (engelsk)Inngår i: Neurogastroenterology and Motility, ISSN 1350-1925, E-ISSN 1365-2982, Vol. 31, nr 1, artikkel-id e13486Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background: Granins have been implicated in the pathophysiology of irritable bowel syndrome (IBS) in adults. We sought to determine whether fecal granins are altered in children with IBS and associated with symptoms.

Methods: Children (7-12 years of age) with IBS and healthy controls (HC) kept daily pain and stool diaries for 2 weeks. Stool samples were analyzed for chromogranins A and B (CgA, CgB) and secretogranins II and III (SgII, SgIII). Children also completed psychological measures to assess anxiety, depression, somatization, and internalizing symptoms.

Key Results: Fecal CgB and SgIII concentrations were higher in all the boys (IBS plus HC, n = 48) than in all the girls (IBS plus HC, n = 75) (P = 0.02 and P = 0.046, respectively). CgA and SgIII were greater in children with IBS (n = 52) vs HC (n = 69) (P = 0.01, P = 0.017, respectively). CgB and SgII did not differ between groups. In children with IBS, the number of pain episodes per week and mean daily pain rating correlated positively with all four granins. The number of stools per day correlated positively with CgB and SgII, and the percent of diarrheal stools (6 or 7 on the Bristol Scale) correlated inversely with all four granins in boys but not in girls. Fecal granins did not correlate with psychological measures.

Conclusions and Inferences: As measured by fecal granins, there is evidence of neuroimmune activation in children with IBS. Granins are related to abdominal pain symptoms, stooling frequency, and stool form in children with IBS. Sex influences the fecal concentration of CgB and SgIII.

sted, utgiver, år, opplag, sider
WILEY, 2019
Emneord
children, chromogranin, irritable bowel syndrome, pediatric, secretogranin
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-372874 (URN)10.1111/nmo.13486 (DOI)000453558600010 ()30298961 (PubMedID)
Tilgjengelig fra: 2019-01-10 Laget: 2019-01-10 Sist oppdatert: 2019-01-10bibliografisk kontrollert
Ottesen, A. H., Carlson, C. R., Eken, O. S., Sadredini, M., Myhre, P. L., Shen, X., . . . Louch, W. E. (2019). Secretoneurin Is an Endogenous Calcium/Calmodulin-Dependent Protein Kinase II Inhibitor That Attenuates Ca2+-Dependent Arrhythmia. Circulation: Arrhythmia and Electrophysiology, 12(4), Article ID e007045.
Åpne denne publikasjonen i ny fane eller vindu >>Secretoneurin Is an Endogenous Calcium/Calmodulin-Dependent Protein Kinase II Inhibitor That Attenuates Ca2+-Dependent Arrhythmia
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2019 (engelsk)Inngår i: Circulation: Arrhythmia and Electrophysiology, ISSN 1941-3149, E-ISSN 1941-3084, Vol. 12, nr 4, artikkel-id e007045Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

BACKGROUND: Circulating SN (secretoneurin) concentrations are increased in patients with myocardial dysfunction and predict poor outcome. Because SN inhibits CaMKII delta (Ca2+/calmodulin-dependent protein kinase II delta) activity, we hypothesized that upregulation of SN in patients protects against cardiomyocyte mechanisms of arrhythmia. METHODS: Circulating levels of SN and other biomarkers were assessed in patients with catecholaminergic polymorphic ventricular tachycardia (CPVT; n=8) and in resuscitated patients after ventricular arrhythmia-induced cardiac arrest (n=155). In vivo effects of SN were investigated in CPVT mice (RyR2 [ryanodine receptor 2]-R2474S) using adeno-associated virus-9-induced overexpression. Interactions between SN and CaMKII delta were mapped using pull-down experiments, mutagenesis, ELISA, and structural homology modeling. Ex vivo actions were tested in Langendorff hearts and effects on Ca2+ homeostasis examined by fluorescence (fluo-4) and patchclamp recordings in isolated cardiomyocytes. RESULTS: SN levels were elevated in patients with CPVT and following ventricular arrhythmia-induced cardiac arrest. In contrast to NT-proBNP (N-terminal proB- type natriuretic peptide) and hs-TnT (high-sensitivity troponin T), circulating SN levels declined after resuscitation, as the risk of a new arrhythmia waned. Myocardial pro-SN expression was also increased in CPVT mice, and further adeno-associated virus-9-induced overexpression of SN attenuated arrhythmic induction during stress testing with isoproterenol. Mechanistic studies mapped SN binding to the substrate binding site in the catalytic region of CaMKII delta. Accordingly, SN attenuated isoproterenol induced autophosphorylation of Thr287-CaMKII delta in Langendorff hearts and inhibited CaMKII delta-dependent RyR phosphorylation. In line with CaMKII delta and RyR inhibition, SN treatment decreased Ca2+ spark frequency and dimensions in cardiomyocytes during isoproterenol challenge, and reduced the incidence of Ca2+ waves, delayed afterdepolarizations, and spontaneous action potentials. SN treatment also lowered the incidence of early afterdepolarizations during isoproterenol; an effect paralleled by reduced magnitude of L-type Ca2+ current. CONCLUSIONS: SN production is upregulated in conditions with cardiomyocyte Ca2+ dysregulation and offers compensatory protection against cardiomyocyte mechanisms of arrhythmia, which may underlie its putative use as a biomarker in at-risk patients.

sted, utgiver, år, opplag, sider
Lippincott Williams & Wilkins, 2019
Emneord
calcium, calmodulin, heart failure, secretoneurin, tachycardia
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-387564 (URN)10.1161/CIRCEP.118.007045 (DOI)000469355500005 ()30943765 (PubMedID)
Forskningsfinansiär
EU, Horizon 2020, 647714
Tilgjengelig fra: 2019-06-25 Laget: 2019-06-25 Sist oppdatert: 2019-06-25bibliografisk kontrollert
Srithunyarat, T., Hagman, R., Hoglund, O. V., Stridsberg, M., Hanson, J., Lagerstedt, A. S. & Pettersson, A. (2018). Catestatin, vasostatin, cortisol, and visual analog scale scoring for stress assessment in healthy dogs. Research in Veterinary Science, 117, 74-80
Åpne denne publikasjonen i ny fane eller vindu >>Catestatin, vasostatin, cortisol, and visual analog scale scoring for stress assessment in healthy dogs
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2018 (engelsk)Inngår i: Research in Veterinary Science, ISSN 0034-5288, E-ISSN 1532-2661, Vol. 117, s. 74-80Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The neuroendocrine glycoprotein chromogranin A is a useful biomarker for stress in humans. Chromogranin A epitopes catestatin and vasostatin can be measured in dogs using radioimmunoassays. The objective of this study was to evaluate catestatin and vasostatin as canine stress biomarkers in a clinical setting. Blood and saliva were collected from 33 healthy dogs that were familiar with sampling procedures and the animal hospital environment (control group) and 30 healthy dogs that were unacquainted (stress group). During sampling, stress behavior was scored by the same observer using visual analog scale (VAS). Plasma was analyzed for catestatin and vasostatin, serum for cortisol, and saliva for catestatin. Differences between groups were analyzed using two sample t-tests and P < 0.05 was considered significant. Stress behavior VAS score in the control group was significantly lower than in the stress group during blood (P = 0.002) and saliva (P = 0.0009) sampling. Serum cortisol and saliva catestatin concentrations in the stress group were higher than the control group (P = 0.003 and P < 0.0001, respectively). Serum cortisol concentrations were correlated with those of saliva (r = 0.34, P = 0.04) and plasma catestatin (r = 0.29, P = 0.03). Plasma catestatin and vasostatin did not differ significantly between groups. In conclusion, concentrations of saliva catestatin, and serum cortisol, and stress behavior VAS scores were significantly higher in the stress group. The results indicate that saliva catestatin may be useful as a biomarker for acute psychological stress in dogs.

Emneord
Biomarker, Canine, Chromogranin A, Psychological stress, Saliva
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-354254 (URN)10.1016/j.rvsc.2017.11.015 (DOI)000430646300011 ()29195227 (PubMedID)
Tilgjengelig fra: 2018-06-29 Laget: 2018-06-29 Sist oppdatert: 2018-06-29bibliografisk kontrollert
Georgantzi, K., Sköldenberg, E., Stridsberg, M., Kogner, P., Jakobson, Å., Tiensuu Janson, E. & Christofferson, R. H. H. (2018). Chromogranin A and neuron-specific enolase in neuroblastoma: Correlation to stage and prognostic factors.. Pediatric Hematology & Oncology, 35(2), 156-165
Åpne denne publikasjonen i ny fane eller vindu >>Chromogranin A and neuron-specific enolase in neuroblastoma: Correlation to stage and prognostic factors.
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2018 (engelsk)Inngår i: Pediatric Hematology & Oncology, ISSN 0888-0018, E-ISSN 1521-0669, Vol. 35, nr 2, s. 156-165Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Chromogranin A (CgA) and neuron specific enolase (NSE) are important markers in adult neuroendocrine tumors (NET). Neuroblastoma (NB) has certain neuroendocrine properties. The aim of this study was to correlate blood concentrations of CgA, chromogranin B (CgB), and NSE to prognostic factors and outcome in children with NB. Blood samples from 92 patients with NB, 12 patients with benign ganglioneuroma (GN), 21 patients with non-NB solid tumors, 10 patients with acute leukemias, and 69 healthy children, were analyzed. CgA concentrations were higher in neonates vs. children older than one month in the control group (p < 0.0001), and in neonates with NB vs. the control group (p < 0.01). CgA and NSE concentrations were higher in patients with stages 3 and 4 disease (p < 0.05 and p < 0.05), in patients having tumors with amplification of MYCN (p < 0.05 and p < 0.001), or chromosome 1 p deletion (p < 0.05 and p < 0.05). NSE correlated to the tumor size at diagnosis (p < 0.001) and to tumor related death (p < 0.01) in NB. CgA and NSE concentrations were elevated in patients with NB and especially in those with advanced disease. Both CgA and NSE correlated to genetic markers, while only NSE correlated to primary tumor size and outcome in NB. We found that CgA and NSE are clinically valuable tumor markers in NB and they merit prospective clinical evaluations as such.

Emneord
Chromogranin A, neuroblastoma, neuron-specific enolase, prognosis, tumor markers
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-360970 (URN)10.1080/08880018.2018.1464087 (DOI)000446356300007 ()29737901 (PubMedID)
Forskningsfinansiär
Swedish Childhood Cancer Foundation
Tilgjengelig fra: 2018-09-20 Laget: 2018-09-20 Sist oppdatert: 2018-12-07bibliografisk kontrollert
Sundin, J., Stridsberg, M., Tap, J., Derrien, M., Le Neve, B., Dore, J., . . . Öhman, L. (2018). Fecal chromogranins and secretogranins are linked to the fecal and mucosal intestinal bacterial composition of IBS patients and healthy subjects. Scientific Reports, 8, Article ID 16821.
Åpne denne publikasjonen i ny fane eller vindu >>Fecal chromogranins and secretogranins are linked to the fecal and mucosal intestinal bacterial composition of IBS patients and healthy subjects
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2018 (engelsk)Inngår i: Scientific Reports, E-ISSN 2045-2322, Vol. 8, artikkel-id 16821Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Altered fecal levels of chromogranins (Cg) and secretogranins (Sg) are demonstrated in irritable bowel syndrome (IBS), but their role in IBS pathophysiology remains unknown. This study aimed to determine if granins are associated with bacterial composition, immune activation and IBS symptoms. Protein levels of fecal granins (CgA, CgB, SgII and SgIII) were analysed with immunoassays. Mucosal mRNA expression of granins, TPH1 and immune markers were evaluated with RT-qPCR. 16S rRNA gene sequencing was performed on fecal and mucosal bacteria. The intestinal granin profile, based on fecal protein levels and mucosal mRNA expression, could not discriminate between IBS patients (n = 88) and healthy subjects (HS, n = 33). IBS patients dominated by high fecal or mucosal granin levels, respectively, did not differ in symptom or immune profiles. Fecal-dominated and mucosal-dominated granin clusters of IBS patients and HS, demonstrated separate fecal and mucosal bacterial profiles and high fecal abundance of granins were associated with a less diverse bacterial composition and the Bacteroides enterotype. The intestinal granin profiles of IBS patients and HS are linked to the intestinal bacterial composition, diversity and enterotypes. These findings suggest that granins may be one of several host-produced factors regulating the microbiota composition of the intestine.

sted, utgiver, år, opplag, sider
NATURE PUBLISHING GROUP, 2018
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-371885 (URN)10.1038/s41598-018-35241-6 (DOI)000450069300042 ()30429499 (PubMedID)
Forskningsfinansiär
VINNOVA, 13409VINNOVA, 21691VINNOVA, 21692
Tilgjengelig fra: 2019-01-03 Laget: 2019-01-03 Sist oppdatert: 2022-09-15bibliografisk kontrollert
Organisasjoner
Identifikatorer
ORCID-id: ORCID iD iconorcid.org/0000-0002-9198-4193