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Florenza, J. & Bertilsson, S. (2023). Choice of methodology and surrogate prey are decisive for the quality of protistan bacterivory rate estimates. Aquatic Microbial Ecology, 89, 43-53
Öppna denna publikation i ny flik eller fönster >>Choice of methodology and surrogate prey are decisive for the quality of protistan bacterivory rate estimates
2023 (Engelska)Ingår i: Aquatic Microbial Ecology, ISSN 0948-3055, E-ISSN 1616-1564, Vol. 89, s. 43-53Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

ABSTRACT: Microeukaryote predation on bacteria is a fundamental phenomenon to understand energy and nutrient dynamics at the base of the aquatic food web. To date, the most prevalent way to estimate grazing rates is by using epifluorescence microscopy to enumerate ingestion events of fluorescently labelled tracers (FLTs) after short-term incubation experiments. However, this approach can be sensitive to the type of FLT, requires skillful preparation of the samples and is limited to small sample sizes. We tested the susceptibility of rate estimates to the choice of prey and made a side-by-side comparison between microscopy and flow cytometry when recording ingestion by a bacterivorous flagellate. Short-term uptake experiments were established using 5 types of FLTs differing in quality (living, dead or inert) and size (large or small), with <i>Ochromonas triangulata</i> as a model flagellate. The experiments showed that (1) each of the different prey types yielded different clearing rates, ranging from 0.5 to 3.6 nl cell<sup>-1</sup> h<sup>-1</sup>, with the largest differences (3-fold or higher) between small prey (lower rates) and large prey (higher rates); (2) the cytometry estimate differed significantly from the microscopy estimate in 3 out of 4 experimental configurations; and (3) the precision of the cytometric analysis was greater, with >3-fold higher uncertainty associated with microscopy counting. Our results validate that flow cytometry provides a more precise bacterivory estimate, and that the choice of FLT influences the grazing rate estimate to a high extent regardless of the analytical method used.

Ort, förlag, år, upplaga, sidor
Inter-Research Science Publisher, 2023
Nyckelord
Bacterivory rates, Fluorescently labelled tracers, FLB, Flow cytometry, Ochromonas triangulata
Nationell ämneskategori
Ekologi Mikrobiologi
Identifikatorer
urn:nbn:se:uu:diva-500711 (URN)10.3354/ame01996 (DOI)000989677200001 ()
Tillgänglig från: 2023-04-21 Skapad: 2023-04-21 Senast uppdaterad: 2023-06-28Bibliografiskt granskad
Wernroth, L., Peura, S., Hedman, A. M., Hetty, S., Vicenzi, S., Kennedy, B., . . . Fall, T. (2022). Development of gut microbiota during the first 2 years of life. Scientific Reports, 12(1), Article ID 9080.
Öppna denna publikation i ny flik eller fönster >>Development of gut microbiota during the first 2 years of life
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2022 (Engelska)Ingår i: Scientific Reports, E-ISSN 2045-2322, Vol. 12, nr 1, artikel-id 9080Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

8Although development of microbiota in childhood has been linked to chronic immune-related conditions, early childhood determinants of microbiota development have not been fully elucidated. We used 16S rRNA sequencing to analyse faecal and saliva samples from 83 children at four time-points during their first 2 years of life and from their mothers. Our findings confirm that gut microbiota in infants have low diversity and highlight that some properties are shared with the oral microbiota, although inter-individual differences are present. A considerable convergence in gut microbiota composition was noted across the first 2 years of life, towards a more diverse adult-like microbiota. Mode of delivery accounted for some of the inter-individual variation in early childhood, but with a pronounced attenuation over time. Our study extends previous research with further characterization of the major shift in gut microbiota composition during the first 2 years of life.

Ort, förlag, år, upplaga, sidor
Springer Nature, 2022
Nationell ämneskategori
Mikrobiologi inom det medicinska området
Identifikatorer
urn:nbn:se:uu:diva-470208 (URN)10.1038/s41598-022-13009-3 (DOI)000803920600048 ()35641542 (PubMedID)
Forskningsfinansiär
Swedish National Infrastructure for Computing (SNIC), sens2018616Vetenskapsrådet, 2018-05973
Tillgänglig från: 2022-03-21 Skapad: 2022-03-21 Senast uppdaterad: 2022-10-20Bibliografiskt granskad
Nota, K., Klaminder, J., Milesi, P., Bindler, R., Nobile, A., van Steijn, T., . . . Parducci, L. (2022). Norway spruce postglacial recolonization of Fennoscandia. Nature Communications, 13, Article ID 1333.
Öppna denna publikation i ny flik eller fönster >>Norway spruce postglacial recolonization of Fennoscandia
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2022 (Engelska)Ingår i: Nature Communications, E-ISSN 2041-1723, Vol. 13, artikel-id 1333Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Contrasting theories exist regarding how Norway spruce (Picea abies) recolonized Fennoscandia after the last glaciation and both early Holocene establishments from western microrefugia and late Holocene colonization from the east have been postulated. Here, we show that Norway spruce was present in southern Fennoscandia as early as 14.7 ± 0.1 cal. kyr BP and that the millennia-old clonal spruce trees present today in central Sweden likely arrived with an early Holocene migration from the east. Our findings are based on ancient sedimentary DNA from multiple European sites (N = 15) combined with nuclear and mitochondrial DNA analysis of ancient clonal (N = 135) and contemporary spruce forest trees (N = 129) from central Sweden. Our other findings imply that Norway spruce was present shortly after deglaciation at the margins of the Scandinavian Ice Sheet, and support previously disputed finds of pollen in southern Sweden claiming spruce establishment during the Lateglacial.

Ort, förlag, år, upplaga, sidor
Springer NatureSpringer Nature, 2022
Nationell ämneskategori
Ekologi
Identifikatorer
urn:nbn:se:uu:diva-467857 (URN)10.1038/s41467-022-28976-4 (DOI)000769063600023 ()35288569 (PubMedID)
Forskningsfinansiär
Vetenskapsrådet, 2017-04548Vetenskapsrådet, 2018-05973Knut och Alice Wallenbergs Stiftelse
Tillgänglig från: 2022-02-17 Skapad: 2022-02-17 Senast uppdaterad: 2024-01-15Bibliografiskt granskad
Patriarca, C., Sedano Núñez, V. T., Garcia, S. L., Bergquist, J., Bertilsson, S., Sjöberg, P. J. R., . . . Hawkes, J. A. (2021). Character and environmental lability of cyanobacteria-derived dissolved organic matter. Limnology and Oceanography, 66(2), 496-509
Öppna denna publikation i ny flik eller fönster >>Character and environmental lability of cyanobacteria-derived dissolved organic matter
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2021 (Engelska)Ingår i: Limnology and Oceanography, ISSN 0024-3590, E-ISSN 1939-5590, Vol. 66, nr 2, s. 496-509Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Autotrophic dissolved organic matter (DOM) is central to the carbon biogeochemistry of aquatic systems, and the full complexity of autotrophic DOM has not been extensively studied, particularly by high‐resolution mass spectrometry (HRMS). Terrestrial DOM tends to dominate HRMS studies in freshwaters due to the propensity of such compounds to ionize by negative mode electrospray, and possibly also because ionizable DOM produced by autotrophy is decreased to low steady‐state concentrations by heterotrophic bacteria. In this study, we investigated the character of DOM produced by the widespread cyanobacteria Microcystis aeruginosa using high‐pressure liquid chromatography—electrospray ionization—high‐resolution mass spectrometry. M. aeruginosa produced thousands of detectable compounds in axenic culture. These compounds were chromatographically resolved and the majority were assigned to aliphatic formulas with a broad polarity range. We found that the DOM produced by M. aeruginosa was highly susceptible to removal by heterotrophic freshwater bacteria, supporting the hypothesis that this autotroph‐derived organic material is highly labile and accordingly only seen at low concentrations in natural settings.

Ort, förlag, år, upplaga, sidor
John Wiley & SonsWiley, 2021
Nationell ämneskategori
Analytisk kemi
Identifikatorer
urn:nbn:se:uu:diva-410263 (URN)10.1002/lno.11619 (DOI)000577965400001 ()
Forskningsfinansiär
Knut och Alice Wallenbergs Stiftelse, 2013.0091Kungliga Vetenskapsakademien, CR2019‐0060Vetenskapsrådet, 2015‐4870Vetenskapsrådet, 2017‐04422Vetenskapsrådet, 2018‐04618
Tillgänglig från: 2020-05-14 Skapad: 2020-05-14 Senast uppdaterad: 2024-01-15Bibliografiskt granskad
Scharnweber, K., Peura, S., Attermeyer, K., Bertilsson, S., Bolender, L., Buck, M., . . . Székely, A. J. (2021). Comprehensive analysis of chemical and biological problems associated with browning agents used in aquatic studies. Limnology and Oceanography: Methods, 19(12), 818-835
Öppna denna publikation i ny flik eller fönster >>Comprehensive analysis of chemical and biological problems associated with browning agents used in aquatic studies
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2021 (Engelska)Ingår i: Limnology and Oceanography: Methods, E-ISSN 1541-5856, Vol. 19, nr 12, s. 818-835Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Inland waters receive and process large amounts of colored organic matter from the terrestrial surroundings. These inputs dramatically affect the chemical, physical, and biological properties of water bodies, as well as their roles as global carbon sinks and sources. However, manipulative studies, especially at ecosystem scale, require large amounts of dissolved organic matter with optical and chemical properties resembling indigenous organic matter. Here, we compared the impacts of two leonardite products (HuminFeed and SuperHume) and a freshly derived reverse osmosis concentrate of organic matter in a set of comprehensive mesocosm- and laboratory-scale experiments and analyses. The chemical properties of the reverse osmosis concentrate and the leonardite products were very different, with leonardite products being low and the reverse osmosis concentrate being high in carboxylic functional groups. Light had a strong impact on the properties of leonardite products, including loss of color and increased particle formation. HuminFeed presented a substantial impact on microbial communities under light conditions, where bacterial production was stimulated and community composition modified, while in dark potential inhibition of bacterial processes was detected. While none of the browning agents inhibited the growth of the tested phytoplankton Gonyostomum semen, HuminFeed had detrimental effects on zooplankton abundance and Daphnia reproduction. We conclude that the effects of browning agents extracted from leonardite, particularly HuminFeed, are in sharp contrast to those originating from terrestrially derived dissolved organic matter. Hence, they should be used with great caution in experimental studies on the consequences of terrestrial carbon for aquatic systems.

Ort, förlag, år, upplaga, sidor
John Wiley & Sons, 2021
Nationell ämneskategori
Ekologi
Identifikatorer
urn:nbn:se:uu:diva-461034 (URN)10.1002/lom3.10463 (DOI)000711887000001 ()
Forskningsfinansiär
Knut och Alice Wallenbergs Stiftelse, KAW 2013.0091Forskningsrådet FormasEU, FP7, Sjunde ramprogrammet
Tillgänglig från: 2021-12-10 Skapad: 2021-12-10 Senast uppdaterad: 2024-01-15Bibliografiskt granskad
Mehrshad, M., Lopez-Fernandez, M., Sundh, J., Bell, E., Simone, D., Buck, M., . . . Dopson, M. (2021). Energy efficiency and biological interactions define the core microbiome of deep oligotrophic groundwater. Nature Communications, 12(1), Article ID 4253.
Öppna denna publikation i ny flik eller fönster >>Energy efficiency and biological interactions define the core microbiome of deep oligotrophic groundwater
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2021 (Engelska)Ingår i: Nature Communications, E-ISSN 2041-1723, Vol. 12, nr 1, artikel-id 4253Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

While oligotrophic deep groundwaters host active microbes attuned to the low-end of the bioenergetics spectrum, the ecological constraints on microbial niches in these ecosystems and their consequences for microbiome convergence are unknown. Here, we provide a genome-resolved, integrated omics analysis comparing archaeal and bacterial communities in disconnected fracture fluids of the Fennoscandian Shield in Europe. Leveraging a dataset that combines metagenomes, single cell genomes, and metatranscriptomes, we show that groundwaters flowing in similar lithologies offer fixed niches that are occupied by a common core microbiome. Functional expression analysis highlights that these deep groundwater ecosystems foster diverse, yet cooperative communities adapted to this setting. We suggest that these communities stimulate cooperation by expression of functions related to ecological traits, such as aggregate or biofilm formation, while alleviating the burden on microorganisms producing compounds or functions that provide a collective benefit by facilitating reciprocal promiscuous metabolic partnerships with other members of the community. We hypothesize that an episodic lifestyle enabled by reversible bacteriostatic functions ensures the subsistence of the oligotrophic deep groundwater microbiome. Ecological constraints on microbial niches in oligotrophic deep groundwaters remain elusive. This study provides support for the existence of a common core microbiome in two deep groundwater biomes of the Fennoscandian Shield using a genome-resolved, integrated omics analysis.

Ort, förlag, år, upplaga, sidor
Springer Nature, 2021
Nationell ämneskategori
Mikrobiologi
Identifikatorer
urn:nbn:se:uu:diva-453773 (URN)10.1038/s41467-021-24549-z (DOI)000675329200009 ()34253732 (PubMedID)
Forskningsfinansiär
Vetenskapsrådet, 2018-04311Vetenskapsrådet, 2017-04422Vetenskapsrådet, 2014-4398VetenskapsrådetSwedish National Infrastructure for Computing (SNIC), b2013127Swedish National Infrastructure for Computing (SNIC), SNIC 2019/3-22Swedish National Infrastructure for Computing (SNIC), SNIC 2020/5-19Knut och Alice Wallenbergs StiftelseSvensk Kärnbränslehantering, SKBCrafoordska stiftelsen, 20180599Crafoordska stiftelsen, 20130557Carl Tryggers stiftelse för vetenskaplig forskning , KF16: 18Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Anmärkning

These authors contributed equally: Maliheh Mehrshad, Margarita Lopez-Fernandez

Tillgänglig från: 2021-10-12 Skapad: 2021-10-12 Senast uppdaterad: 2024-01-15Bibliografiskt granskad
Garcia, S. L., Mehrshad, M., Buck, M., Tsuji, J. M., Neufeld, J. D., McMahon, K. D., . . . Peura, S. (2021). Freshwater Chlorobia Exhibit Metabolic Specialization among Cosmopolitan and Endemic Populations. mSystems, 6(3), Article ID e01196-20.
Öppna denna publikation i ny flik eller fönster >>Freshwater Chlorobia Exhibit Metabolic Specialization among Cosmopolitan and Endemic Populations
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2021 (Engelska)Ingår i: mSystems, E-ISSN 2379-5077, Vol. 6, nr 3, artikel-id e01196-20Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Photosynthetic bacteria from the class Chlorobia (formerly phylum Chlorobi) sustain carbon fixation in anoxic water columns. They harvest light at extremely low intensities and use various inorganic electron donors to fix carbon dioxide into biomass. Until now, most information on the functional ecology and local adaptations of Chlorobia members came from isolates and merely 26 sequenced genomes that may not adequately represent natural populations. To address these limitations, we analyzed global metagenomes to profile planktonic Chlorobia cells from the oxyclines of 42 freshwater bodies, spanning subarctic to tropical regions and encompassing all four seasons. We assembled and compiled over 500 genomes, including metagenome-assembled genomes (MAGs), single-amplified genomes (SAGs), and reference genomes from cultures, clustering them into 71 metagenomic operational taxonomic units (mOTUs or “species”). Of the 71 mOTUs, 57 were classified within the genus Chlorobium, and these mOTUs represented up to ∼60% of the microbial communities in the sampled anoxic waters. Several Chlorobium-associated mOTUs were globally distributed, whereas others were endemic to individual lakes. Although most clades encoded the ability to oxidize hydrogen, many lacked genes for the oxidation of specific sulfur and iron substrates. Surprisingly, one globally distributed Scandinavian clade encoded the ability to oxidize hydrogen, sulfur, and iron, suggesting that metabolic versatility facilitated such widespread colonization. Overall, these findings provide new insight into the biogeography of the Chlorobia and the metabolic traits that facilitate niche specialization within lake ecosystems.

IMPORTANCE The reconstruction of genomes from metagenomes has helped explore the ecology and evolution of environmental microbiota. We applied this approach to 274 metagenomes collected from diverse freshwater habitats that spanned oxic and anoxic zones, sampling seasons, and latitudes. We demonstrate widespread and abundant distributions of planktonic Chlorobia-associated bacteria in hypolimnetic waters of stratified freshwater ecosystems and show they vary in their capacities to use different electron donors. Having photoautotrophic potential, these Chlorobia members could serve as carbon sources that support metalimnetic and hypolimnetic food webs.

Ort, förlag, år, upplaga, sidor
American Society for MicrobiologyAmerican Society for Microbiology, 2021
Nyckelord
Chlorobia, freshwater, photosynthetic bacteria, planktonic
Nationell ämneskategori
Mikrobiologi
Identifikatorer
urn:nbn:se:uu:diva-458706 (URN)10.1128/mSystems.01196-20 (DOI)000709811800011 ()33975970 (PubMedID)
Forskningsfinansiär
Knut och Alice Wallenbergs Stiftelse, KAW 2013.0091VetenskapsrådetForskningsrådet FormasSwedish National Infrastructure for Computing (SNIC), SNIC2020/5-19Science for Life Laboratory, SciLifeLabKungliga Vetenskapsakademien
Tillgänglig från: 2021-11-25 Skapad: 2021-11-25 Senast uppdaterad: 2024-01-15Bibliografiskt granskad
Urrutia Cordero, P., Langenheder, S., Striebel, M., Eklöv, P., Angeler, D. G., Bertilsson, S., . . . Hillebrand, H. (2021). Functionally reversible impacts of disturbances on lake food webs linked to spatial and seasonal dependencies. Ecology, 102(4), Article ID 03283.
Öppna denna publikation i ny flik eller fönster >>Functionally reversible impacts of disturbances on lake food webs linked to spatial and seasonal dependencies
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2021 (Engelska)Ingår i: Ecology, ISSN 0012-9658, E-ISSN 1939-9170, Vol. 102, nr 4, artikel-id 03283Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Increasing human impact on the environment is causing drastic changes in disturbance regimes and how they prevail over time. Of increasing relevance is to further our understanding on biological responses to pulse disturbances (short duration) and how they interact with other ongoing press disturbances (constantly present). Because the temporal and spatial contexts of single experiments often limit our ability to generalize results across space and time, we conducted a modularized mesocosm experiment replicated in space (five lakes along a latitudinal gradient in Scandinavia) and time (two seasons, spring and summer) to generate general predictions on how the functioning and composition of multitrophic plankton communities (zoo-, phyto- and bacterioplankton) respond to pulse disturbances acting either in isolation or combined with press disturbances. As pulse disturbance, we used short-term changes in fish presence, and as press disturbance, we addressed the ongoing reduction in light availability caused by increased cloudiness and lake browning in many boreal and subarctic lakes. First, our results show that the top-down pulse disturbance had the strongest effects on both functioning and composition of the three trophic levels across sites and seasons, with signs for interactive impacts with the bottom-up press disturbance on phytoplankton communities. Second, community composition responses to disturbances were highly divergent between lakes and seasons: temporal accumulated community turnover of the same trophic level either increased (destabilization) or decreased (stabilization) in response to the disturbances compared to control conditions. Third, we found functional recovery from the pulse disturbances to be frequent at the end of most experiments. In a broader context, these results demonstrate that top-down, pulse disturbances, either alone or with additional constant stress upon primary producers caused by bottom-up disturbances, can induce profound but often functionally reversible changes across multiple trophic levels, which are strongly linked to spatial and temporal context dependencies. Furthermore, the identified dichotomy of disturbance effects on the turnover in community composition demonstrates the potential of disturbances to either stabilize or destabilize biodiversity patterns over time across a wide range of environmental conditions.

Ort, förlag, år, upplaga, sidor
John Wiley & SonsWILEY, 2021
Nyckelord
biodiversity, community composition, community turnover, disturbances, ecosystem functioning, global change, lakes, plankton, recovery
Nationell ämneskategori
Ekologi
Identifikatorer
urn:nbn:se:uu:diva-446645 (URN)10.1002/ecy.3283 (DOI)000623058600001 ()33428769 (PubMedID)
Forskningsfinansiär
VetenskapsrådetVetenskapsrådet, 2017-06421
Tillgänglig från: 2021-06-22 Skapad: 2021-06-22 Senast uppdaterad: 2024-01-15Bibliografiskt granskad
Jingying, X., Liem-Nguyen, V., Buck, M., Bertilsson, S., Björn, E. & Bravo, A. G. (2021). Mercury Methylating Microbial Community Structure in Boreal Wetlands Explained by Local Physicochemical Conditions. Frontiers in Environmental Science, 8, Article ID 518662.
Öppna denna publikation i ny flik eller fönster >>Mercury Methylating Microbial Community Structure in Boreal Wetlands Explained by Local Physicochemical Conditions
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2021 (Engelska)Ingår i: Frontiers in Environmental Science, E-ISSN 2296-665X, Vol. 8, artikel-id 518662Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The potent neurotoxin methylmercury (MeHg) is a major concern due to its negative effects on wildlife and human health. Boreal wetlands play a crucial role in Hg cycling on a global scale, and therefore, it is crucial to understand the biogeochemical processes involved in MeHg formation in this landscape element. By combining high-throughput hgcA amplicon sequencing with molecular barcoding, we reveal diverse clades of potential Hg-II methylators in a wide range of wetland soils. Among Bacteria, Desulfuromonadota (14% of total reads), Desulfurobacterota_A, and Desulfurobacterota (up to 6% of total reads), previously classified as Deltaproteobacteria, were important members of the hgcA+ microbial community in the studied wetlands. We also identified Actinobacteriota (9.4% of total reads), Bacteroidota (2% of total reads), and Firmicutes (1.2% of total reads) as members of the hgcA+ microbial community. Within Archaea, Methanosarcinales represented up to 2.5% of the total reads. However, up to half of the hgcA+ community could not be resolved beyond domain Bacteria. Our survey also shows that local physicochemical conditions, such as pH, nutrient concentrations, water content, and prevailing redox states, are important for shaping the hgcA+ microbial community structure across the four studied wetlands. Furthermore, we observed a significant correlation between Hg-II methylation rate constants and the structure of the hgcA+ microbial community. Our findings expand the current knowledge on the hgcA+ microbial community composition in wetlands and the physicochemical factors underpinning spatial heterogeneity in such communities.

Ort, förlag, år, upplaga, sidor
Frontiers Media S.A.FRONTIERS MEDIA SA, 2021
Nyckelord
wetlands, methylmercury, mercury methylation, hgcA, community composition, bacteria, mercury, drivers
Nationell ämneskategori
Mikrobiologi Miljövetenskap
Identifikatorer
urn:nbn:se:uu:diva-439823 (URN)10.3389/fenvs.2020.518662 (DOI)000625143900001 ()
Forskningsfinansiär
Vetenskapsrådet, 2011-7192Vetenskapsrådet, 2012-3892Vetenskapsrådet, 2017-04422Vetenskapsrådet, 2013-6978Kempestiftelserna, SMK-2745Kempestiftelserna, SMK-1243EU, Horisont 2020, H2020-MSCA-IF-2016Wenner-Gren Stiftelserna
Tillgänglig från: 2021-04-12 Skapad: 2021-04-12 Senast uppdaterad: 2024-01-15Bibliografiskt granskad
Urrutia Cordero, P., Langvall, O., Blomkvist, P., Angeler, D. G., Bertilsson, S., Colom-Montero, W., . . . Langenheder, S. (2021). SITES AquaNet: An open infrastructure for mesocosm experiments with high frequency sensor monitoring across lakes. Limnology and Oceanography: Methods, 19(6), 385-400
Öppna denna publikation i ny flik eller fönster >>SITES AquaNet: An open infrastructure for mesocosm experiments with high frequency sensor monitoring across lakes
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2021 (Engelska)Ingår i: Limnology and Oceanography: Methods, E-ISSN 1541-5856, Vol. 19, nr 6, s. 385-400Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

For aquatic scientists mesocosm experiments are important tools for hypothesis testing as they offer a compromise between experimental control and realism. Here we present a new mesocosm infrastructure-SITES AquaNET-located in five lakes connected to field stations in Sweden that cover a similar to 760 km latitudinal gradient. SITES AquaNet overcomes major hindrances in aquatic experimental research through: (i) openness to the scientific community, (ii) the potential to implement coordinated experiments across sites and time, and (iii) high-frequency measurements (temperature, photosynthetic photon flux density, turbidity and dissolved oxygen, chlorophyll a and phycocyanin concentrations) with an autonomous sensor system. Moreover, the infrastructure provides operational guidance and sensor expertise from technical staff, and connections to a multi-layered monitoring programme ("SITES Water") for each lake. This enables ecological observations from whole lake ecosystems to be compared with experimental studies aiming at disentangling major drivers and mechanisms underlying observed changes. Here we describe the technical properties of the infrastructure along with possibilities for experimental manipulations to tackle pressing issues in aquatic ecology and global change science. As a proof of concept, we also present a first mesocosm experiment across all five field sites with a cross-factorial design to evaluate responses of the sensor measurements to press/bottom-up (constant light reduction) and pulse/top-down (temporary fish predation) disturbances. This demonstrates the suitability of the infrastructure and autonomous sensor system to host modularized experiments and exemplifies the power and advantages of the approach to integrate a network of mecsocosm facilities with manageable costs across large geographic areas.

Ort, förlag, år, upplaga, sidor
John Wiley & SonsWILEY, 2021
Nationell ämneskategori
Oceanografi, hydrologi och vattenresurser Ekologi
Identifikatorer
urn:nbn:se:uu:diva-454291 (URN)10.1002/lom3.10432 (DOI)000647918900001 ()
Forskningsfinansiär
Vetenskapsrådet, 2017-06421
Tillgänglig från: 2021-09-28 Skapad: 2021-09-28 Senast uppdaterad: 2024-01-15Bibliografiskt granskad
Projekt
Ansökan om förlängning av anställning som forskare i ämnet Biogeokemiska processer - småskaliga mekanismer med effekt på storskaliga stystem. [2008-01293_VR]; Uppsala universitetHur dynamiska är mikrobiella samhällen i sjöekosystem? -Ett ekologiskt observatorium för att studera mikrobiella samhällens variation över dygn och säsonger [2008-04359_VR]; Uppsala universitetInternationellt forskningssamarbeta med fokus på Amundsenhavet (ASPIRE): Mikrobiella samhällens variation och betydelse för kolomsättningen i södra ishavet [2008-06430_VR]; Uppsala universitetSjöbakteriers utnyttjande av organiska föreningar: specialister eller generalister? [2009-03784_VR]; Uppsala universitetHur vidmakthålls nedbrytning av organiskt material över redox gradienter: samhällsförändringar eller anpassning? [2012-03892_VR]; Uppsala universitetCellsortering för enkelcellsgenomik i miljöprov [2012-05095_VR]; Uppsala universitetPHYTOREV: A coordinated effort to understand genomic changes in revived diatom and phytoplankton populations from Baltic Sea sediments in light of environmental change [21-RN-0003_OSS]; Södertörns högskola
Organisationer
Identifikatorer
ORCID-id: ORCID iD iconorcid.org/0000-0002-4265-1835

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