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Åkerblom, O.
Alternative names
Publications (3 of 3) Show all publications
Norda, R., Schött, U., Berseus, O., Åkerblom, O., Nilsson, B., Nilsson Ekdahl, K., . . . Knutson, F. (2012). Complement activation products in liquid stored plasma and C3a-kinetics after transfusion of autologous plasma. Vox Sanguinis, 102(2), 125-133
Open this publication in new window or tab >>Complement activation products in liquid stored plasma and C3a-kinetics after transfusion of autologous plasma
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2012 (English)In: Vox Sanguinis, ISSN 0042-9007, E-ISSN 1423-0410, Vol. 102, no 2, p. 125-133Article in journal (Refereed) Published
Abstract [en]

Background and Objectives  Keeping a small stock of liquid plasma readily available for transfusion is common practise in Sweden. We report data on complement activation markers in plasma components during storage in the liquid state and the kinetics of C3a-(desArg) after transfusion of autologous plasma with high content of C3a-(desArg) . Material and Methods  Plasma components were prepared by apheresis or from whole blood. C3 fragments (C3a-(desArg) , C3d,g, iC3), and soluble terminal complement complex (sC5b-9) were investigated. C3a-(desArg) kinetics was investigated in regular apheresis donors. Results  Apheresis plasma prepared by membrane centrifugation had significantly higher level of C3a-(desArg) , C3d,g and sC5b-9 from day 0 and low iC3, than plasma prepared by other methods. By storage day 7, C3a-(desArg) -levels were above the reference value in 88% of all components. After re-infusion of autologous plasma with high C3a-(desArg) content, there were rapid a(1) and a(2) -distribution followed by a slower b-elimination phase. Conclusion  Plasma components prepared by different methods and stored in the liquid phase differ significantly in the amount and timing of complement activation. C3a-(desArg) present in plasma is rapidly eliminated after transfusion. Autologous plasma could be used to study complement kinetics in different clinical situations.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-159755 (URN)10.1111/j.1423-0410.2011.01522.x (DOI)000299206300005 ()21770955 (PubMedID)
Available from: 2011-10-10 Created: 2011-10-10 Last updated: 2017-12-08Bibliographically approved
Norda, R., Knutson, F., Berseus, O., Åkerblom, O., Nilsson-Ekdahl, K., Stegmayr, B. & Nilsson, B. (2007). Unexpected effects of donor gender on the storage of liquid plasma. Vox Sanguinis, 93(3), 223-228
Open this publication in new window or tab >>Unexpected effects of donor gender on the storage of liquid plasma
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2007 (English)In: Vox Sanguinis, ISSN 0042-9007, E-ISSN 1423-0410, Vol. 93, no 3, p. 223-228Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Swedish regulations in effect since 2006 allow the storage of plasma for transfusion up to 14 days at 2-6 degrees C and for 3 years at < or = -30 degrees C. In this study, the quality of currently used plasma components was investigated. MATERIALS AND METHODS: Plasma components, prepared from whole blood or by apheresis, either leucocyte depleted or not leucocyte depleted, were stored at 2-6 degrees C as liquid plasma or as thawed fresh-frozen plasma; 31% were from female donors. Concentration, function and activation markers of the plasma coagulation systems were investigated during storage for up to 42 days. RESULTS: Cold-induced contact activation was the dominant storage lesion, occurring earlier and at higher frequency in plasma from females. Increased kallikrein-like activity led to changes in activated partial thromboplastin time, prothrombin time, protein C and C1 inhibitor (C1INH). C1INH function dropped to 53% on Day 14 in cold-activated plasma components. CONCLUSION: Contact activation may be triggered before Day 14, especially in plasma from females, and may progress as a result of the consumption of C1INH. The data suggest that lack of cold-induced contact activation may be an important quality criterion. To achieve this, plasma from male donors could be selected for transfusion and the storage time limited to 7 days.

Keywords
C1 inhibitor, Contact activation, Donor gender, Long-term storage, Plasma preparation
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-16836 (URN)10.1111/j.1423-0410.2007.00963.x (DOI)000249436600007 ()17845259 (PubMedID)
Available from: 2008-06-05 Created: 2008-06-05 Last updated: 2017-12-08Bibliographically approved
Annuk, M., Fellström, B., Åkerblom, O., Zilmer, K., Vihalemm, T. & Zilmer, M. (2001). Oxidative stress markers in pre-uremic patients. Clinical Nephrology, 56(4), 308-314
Open this publication in new window or tab >>Oxidative stress markers in pre-uremic patients
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2001 (English)In: Clinical Nephrology, ISSN 0301-0430, Vol. 56, no 4, p. 308-314Article in journal (Other (popular science, discussion, etc.)) Published
Abstract [en]

AIM: The present study was designed to investigate a complex of oxidative stress (OS) markers in patients with chronic renal failure (CRF) and to study the relationship between different OS markers and degree of renal failure. The following indices of OS were measured in plasma: oxidized glutathione (GSSG), reduced glutathione (GSH), total glutathione (TGSH), glutathione redox ratio (GSSG/GSH) and resistance of lipoprotein fraction to oxidation (lag phase of LPF). Baseline diene conjugation level of lipoprotein fraction (BDC-LPF), total antioxidative activity (TAA), diene conjugates (DC), lipid hydroperoxides (LOOH) and thiobarbituric acid-reactive substances (TBARS) were measured in serum. All markers in plasma and serum were measured both in patients with CRF and in healthy controls.

SUBJECTS AND METHODS: Blood samples were obtained from 38 patients with CRF and from 61 healthy controls. Routine biochemical analyses were performed by using commercially available kits.

RESULTS: Levels of DC, BDC-LPF, LOOH, GSSG and GSSG/GSH ratio were significantly increased and lag phase of LPF was significantly shortened in patients with CRF compared with healthy controls. Serum creatinine and urea levels correlated significantly with GSSG level and GSSG/GSH in patients with CRF. A significant inverse correlation was found between glutathione redox ratio and lag phase of LPF and between GSSG level and BDC-LPF.

CONCLUSIONS: The findings suggest that renal patients are in a state of oxidative stress compared with healthy controls. The most informative indices to evaluate the degree of OS in CRF were: GSSG level, GSSG/GSH status, lag phase of LPF and BDC-LPF.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-72212 (URN)11680661 (PubMedID)
Available from: 2005-09-18 Created: 2005-09-18 Last updated: 2017-12-14Bibliographically approved
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