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Sharma, Hari Shanker
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Publications (10 of 193) Show all publications
Wiklund, L., Patnaik, R., Sharma, A., Miclescu, A. & Sharma, H. S. (2017). Cerebral Tissue Oxidative Ischemia-Reperfusion Injury in Connection with Experimental Cardiac Arrest and Cardiopulmonary Resuscitation: Effect of Mild Hypothermia and Methylene Blue. Molecular Neurobiology.
Open this publication in new window or tab >>Cerebral Tissue Oxidative Ischemia-Reperfusion Injury in Connection with Experimental Cardiac Arrest and Cardiopulmonary Resuscitation: Effect of Mild Hypothermia and Methylene Blue
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2017 (English)In: Molecular Neurobiology, ISSN 0893-7648, E-ISSN 1559-1182Article in journal (Refereed) Epub ahead of print
Abstract [en]

The present investigation is an expansion of previous studies which all share a basic experimental protocol of a porcine-induced cardiac arrest (CA) of 12 min followed by 8 min of cardiopulmonary resuscitation (CPR), different experimental treatments (immediate as well as postponed induced mild hypothermia and administration of much or less cool intravenous fluids), and a follow-up period of 3 h after which the animals were sacrificed. Another group of animals was studied according to the same protocol after 12-min CA and Bstandard CPR.^ After death (within 1 min), the brains were harvested and frozen in liquid nitrogen awaiting analysis. Control brains of animals were collected in the same way after short periods of untreated CA (0 min, 5 min, and 15–30 min). Previous studies concerning chiefly neuropathological changes were now expanded with analyses of different tissue indicators (glutathione, luminol, leucigenin, malonialdehyde, and myeloperoxidase) of cerebral oxidative injury. The results indicate that a great part of oxidative injury occurs within the first 5 min after CA. Immediate cooling by administration of much intravenous fluid results in less cerebral oxidative injury compared to less intravenous fluid administration. A 30-min postponement of induction of hypothermia results in a cerebral oxidative injury comparable to that of Bstandard CPR^ or the oxidative injury found after 5 min of untreated CA. Intravenous administration of methylene blue (MB) during and immediately after CPR in combination with postponed cooling resulted in no statistical difference in any of the indicators of oxidative injury, except myeloperoxidase, and glutathione, when this treatment was compared with the negative controls, i.e., animals subjected to anesthesia alone.

Keyword
Cardiac arrest, Oxidative injury, Ischemia reperfusion, Methylene blue
National Category
Anesthesiology and Intensive Care
Identifiers
urn:nbn:se:uu:diva-337384 (URN)10.1007/s12035-017-0723-z (DOI)
Available from: 2017-12-24 Created: 2017-12-24 Last updated: 2018-01-04Bibliographically approved
Sharma, A., Muresanu, D. F. & Sharma, H. S. (2017). Co-administration of nanowired mesenchymal stem cells and cerebrolysin potentiates neuroprotection in Parkinsons disease following mild traumatic brain injury. Paper presented at 28th International Symposium on Cerebral Blood Flow, Metabolism and Function / 13th International Conference on Quantification of Brain Function with PET, APR 01-04, 2017, Int Soc Cerebral Blood Flow & Metab, Berlin, GERMANY. Journal of Cerebral Blood Flow and Metabolism, 37, 19-19.
Open this publication in new window or tab >>Co-administration of nanowired mesenchymal stem cells and cerebrolysin potentiates neuroprotection in Parkinsons disease following mild traumatic brain injury
2017 (English)In: Journal of Cerebral Blood Flow and Metabolism, ISSN 0271-678X, E-ISSN 1559-7016, Vol. 37, 19-19 p.Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
SAGE PUBLICATIONS INC, 2017
National Category
Endocrinology and Diabetes Hematology Neurology
Identifiers
urn:nbn:se:uu:diva-331028 (URN)000400157400026 ()
Conference
28th International Symposium on Cerebral Blood Flow, Metabolism and Function / 13th International Conference on Quantification of Brain Function with PET, APR 01-04, 2017, Int Soc Cerebral Blood Flow & Metab, Berlin, GERMANY
Available from: 2017-10-10 Created: 2017-10-10 Last updated: 2017-10-10
Zhang, Z., Li, C., Tan, Q., Xie, C., Yang, Y., Zhan, W., . . . Sharma, A. (2017). Curcumin Suppresses Tumor Growth and Angiogenesis in Human Glioma Cells Through Modulation of Vascular Endothelial Growth Factor/Angiopoietin-2/Thrombospondin-1 Signaling. CNS & Neurological Disorders: Drug Targets, 16(3), 346-350.
Open this publication in new window or tab >>Curcumin Suppresses Tumor Growth and Angiogenesis in Human Glioma Cells Through Modulation of Vascular Endothelial Growth Factor/Angiopoietin-2/Thrombospondin-1 Signaling
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2017 (English)In: CNS & Neurological Disorders: Drug Targets, ISSN 1871-5273, E-ISSN 1996-3181, Vol. 16, no 3, 346-350 p.Article in journal (Refereed) Published
Abstract [en]

Purpose: To investigate the effect of curcumin on tumor growth and angiogenesis of human gliomas and identify the underlying molecular mechanisms. Methods: A mouse xenograft glioma model was established by subcutaneously inoculating tumor cell aggregates derived from the U87 cell line. Mice were treated with 0.01ml/g body weight of curcumin or saline. Tumor volume was measured. Microvessel density was assessed by CD34 immunostaining, and angiogenesis by immunohistochemical staining of vascular endothelial growth factor (VEGF), angiopoietin-2 (Ang-2) and thrombospondin 1 (TSP-1). Results: At 28 days after treatment, tumor weights in the curcumin-treated group were much smaller than in the control group (0.23 +/- 0.11g vs. 0.44 +/- 0.15g p < 0.05), resulting in a 45.8% inhibition of tumor growth. Curcumin also markedly inhibited microvessel density. Expression of VEGF and Ang-2 was inhibited by curcumin, whereas TSP-1 expression was up-regulated. Conclusion: This study shows that curcumin inhibits tumor growth by inhibiting VEGF/Ang-2/TSP-1-mediated angiogenesis in a xenograft glioma mouse model.

Keyword
Curcumin, glioma, angiogenesis, vascular endothelial growth factor
National Category
Pharmacology and Toxicology Neurosciences
Identifiers
urn:nbn:se:uu:diva-331958 (URN)10.2174/1871527315666160902144513 (DOI)000405316600013 ()
Funder
Swedish Foundation for Strategic Research
Available from: 2017-10-20 Created: 2017-10-20 Last updated: 2018-01-13Bibliographically approved
Sharma, H. S., Sharma, A. & Muresanu, D. F. (2017). Neuroprotective effects of nanowired cerebrolysin in regional cerebral blood flow disturbances, blood-brain barrier breakdown, edema formation and brain pathology following a focal blast brain injury. Paper presented at 28th International Symposium on Cerebral Blood Flow, Metabolism and Function / 13th International Conference on Quantification of Brain Function with PET, APR 01-04, 2017, Int Soc Cerebral Blood Flow & Metab, Berlin, GERMANY. Journal of Cerebral Blood Flow and Metabolism, 37, 130-130.
Open this publication in new window or tab >>Neuroprotective effects of nanowired cerebrolysin in regional cerebral blood flow disturbances, blood-brain barrier breakdown, edema formation and brain pathology following a focal blast brain injury
2017 (English)In: Journal of Cerebral Blood Flow and Metabolism, ISSN 0271-678X, E-ISSN 1559-7016, Vol. 37, 130-130 p.Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
Sage Publications, 2017
National Category
Endocrinology and Diabetes Hematology Neurology
Identifiers
urn:nbn:se:uu:diva-331034 (URN)000400157400187 ()
Conference
28th International Symposium on Cerebral Blood Flow, Metabolism and Function / 13th International Conference on Quantification of Brain Function with PET, APR 01-04, 2017, Int Soc Cerebral Blood Flow & Metab, Berlin, GERMANY
Note

Supplement: 1, Meeting Abstract: PS01-054

Available from: 2017-10-11 Created: 2017-10-11 Last updated: 2017-10-11
Muresanu, D. F., Sharma, A. & Sharma, H. S. (2017). Pathophysiology of high altitude traumatic brain edema: New roles of cererbrolysin and nanomedicine. Paper presented at 28th International Symposium on Cerebral Blood Flow, Metabolism and Function / 13th International Conference on Quantification of Brain Function with PET, APR 01-04, 2017, Int Soc Cerebral Blood Flow & Metab, Berlin, GERMANY. Journal of Cerebral Blood Flow and Metabolism, 37, 208-209.
Open this publication in new window or tab >>Pathophysiology of high altitude traumatic brain edema: New roles of cererbrolysin and nanomedicine
2017 (English)In: Journal of Cerebral Blood Flow and Metabolism, ISSN 0271-678X, E-ISSN 1559-7016, Vol. 37, 208-209 p.Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
Sage Publications, 2017
National Category
Endocrinology and Diabetes Hematology Neurology
Identifiers
urn:nbn:se:uu:diva-331035 (URN)000400157400305 ()
Conference
28th International Symposium on Cerebral Blood Flow, Metabolism and Function / 13th International Conference on Quantification of Brain Function with PET, APR 01-04, 2017, Int Soc Cerebral Blood Flow & Metab, Berlin, GERMANY
Note

Supplement: 1, Meeting Abstract: PS02-065

Available from: 2017-10-11 Created: 2017-10-11 Last updated: 2017-10-11
Sharma, H. S. & Sharma, A. (2016). 5th International Conference on Nanotek & Expo, Nov 16-18, 2015, San Antonio TX, USA. CNS & Neurological Disorders: Drug Targets, 15(3), 265-266.
Open this publication in new window or tab >>5th International Conference on Nanotek & Expo, Nov 16-18, 2015, San Antonio TX, USA
2016 (English)In: CNS & Neurological Disorders: Drug Targets, ISSN 1871-5273, E-ISSN 1996-3181, Vol. 15, no 3, 265-266 p.Article in journal, Editorial material (Other academic) Published
National Category
Neurology Pharmacology and Toxicology
Identifiers
urn:nbn:se:uu:diva-299124 (URN)10.2174/187152731503160310160842 (DOI)000373801100002 ()27009119 (PubMedID)
Available from: 2016-07-14 Created: 2016-07-14 Last updated: 2018-01-10Bibliographically approved
Sharma, H. S., Skaper, S. D. & Sharma, A. (2016). 8th Clinical Trials on Alzheimer Disease (CTAD), Barcelona, Spain November 5-7, 2015. CNS & Neurological Disorders: Drug Targets, 15(4), 375-377.
Open this publication in new window or tab >>8th Clinical Trials on Alzheimer Disease (CTAD), Barcelona, Spain November 5-7, 2015
2016 (English)In: CNS & Neurological Disorders: Drug Targets, ISSN 1871-5273, E-ISSN 1996-3181, Vol. 15, no 4, 375-377 p.Article in journal, Editorial material (Other academic) Published
National Category
Neurology Pharmacology and Toxicology
Identifiers
urn:nbn:se:uu:diva-299125 (URN)10.2174/187152731504160328164331 (DOI)000373802500002 ()27040759 (PubMedID)
Available from: 2016-07-14 Created: 2016-07-14 Last updated: 2018-01-10Bibliographically approved
Sharma, H. S., Muresanu, D. F. & Sharma, A. (2016). Alzheimer's disease: cerebrolysin and nanotechnology as a therapeutic strategy. NEURODEGENERATIVE DISEASE MANAGEMENT, 6(6), 453-456.
Open this publication in new window or tab >>Alzheimer's disease: cerebrolysin and nanotechnology as a therapeutic strategy
2016 (English)In: NEURODEGENERATIVE DISEASE MANAGEMENT, ISSN 1758-2024, Vol. 6, no 6, 453-456 p.Article in journal, Editorial material (Refereed) Published
Keyword
A beta P infusion, Alzheimer's disease, BBB leakage, brain pathology, cerebrolysin, diabetes, hypertension, military personnel, model, nanodelivery, neuroprotection, neurotrophic factors, post, traumatic stress disorder, traumatic brain injury
National Category
Neurology
Identifiers
urn:nbn:se:uu:diva-313843 (URN)10.2217/nmt-2016-0037 (DOI)000389768200002 ()27827552 (PubMedID)
Available from: 2017-01-26 Created: 2017-01-25 Last updated: 2017-01-26Bibliographically approved
Kiyatkin, E. A. & Sharma, H. S. (2016). Breakdown of Blood-Brain and Blood-Spinal Cord Barriers During Acute Methamphetamine Intoxication: Role of Brain Temperature. CNS & Neurological Disorders: Drug Targets, 15(9), 1129-1138.
Open this publication in new window or tab >>Breakdown of Blood-Brain and Blood-Spinal Cord Barriers During Acute Methamphetamine Intoxication: Role of Brain Temperature
2016 (English)In: CNS & Neurological Disorders: Drug Targets, ISSN 1871-5273, E-ISSN 1996-3181, Vol. 15, no 9, 1129-1138 p.Article, review/survey (Refereed) Published
Abstract [en]

Methamphetamine (METH) is a powerful and often-abused stimulant with potent addictive and neurotoxic properties. While it is generally believed that structural brain damage induced by METH results from oxidative stress, in this work we present data suggesting robust disruption of blood-brain and blood-spinal cord barriers during acute METH intoxication in rats. We demonstrate the relationships between METH-induced brain hyperthermia and widespread but structure-specific barrier leakage, acute glial cell activation, changes in brain water and ionic homeostasis, and structural damage of different types of cells in the brain and spinal cord. Therefore, METH-induced leakage of the blood-brain and blood-spinal cord barriers is a significant contributor to different types of functional and structural brain abnormalities that determine acute toxicity of this drug and possibly neurotoxicity during its chronic use.

Keyword
Brain edema, brain hyperthermia, cellular damage, methamphetamine, neurotoxicity, psychomotor stimulants, skin vasoconstriction
National Category
Neurology Pharmacology and Toxicology
Identifiers
urn:nbn:se:uu:diva-311091 (URN)10.2174/1871527315666160920112445 (DOI)000387125600010 ()27658516 (PubMedID)
Funder
NIH (National Institute of Health)
Available from: 2016-12-21 Created: 2016-12-21 Last updated: 2018-01-13Bibliographically approved
Sharma, A., Muresanu, D., Lafuente, J. V., Patnaik, R., Tian, Z. R., Moessler, H. & Sharma, H. S. (2016). Cold environment exacerbates brain pathology and oxidative stress following traumatic brain injuries. Potential therapeutic effects of nanowired cerebrolysin. Brain Injury, 30(5-6), 506-506.
Open this publication in new window or tab >>Cold environment exacerbates brain pathology and oxidative stress following traumatic brain injuries. Potential therapeutic effects of nanowired cerebrolysin
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2016 (English)In: Brain Injury, ISSN 0269-9052, E-ISSN 1362-301X, Vol. 30, no 5-6, 506-506 p.Article in journal, Meeting abstract (Other academic) Published
National Category
Neurology
Identifiers
urn:nbn:se:uu:diva-299657 (URN)000376388200063 ()
Available from: 2016-07-25 Created: 2016-07-25 Last updated: 2017-11-28Bibliographically approved
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