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Herrmann, Björn
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Publications (10 of 71) Show all publications
Wang, H., Jensen, J.-K., Olsson, A., Vorimore, F., Aaziz, R., Guy, L., . . . Herrmann, B. (2020). Chlamydia psittaci in Fulmars on The Faroe Islands: A Causative Link to South American Psittacines Eight Decades After a Severe Epidemic. Microbes and infection
Open this publication in new window or tab >>Chlamydia psittaci in Fulmars on The Faroe Islands: A Causative Link to South American Psittacines Eight Decades After a Severe Epidemic
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2020 (English)In: Microbes and infection, ISSN 1286-4579, E-ISSN 1769-714XArticle in journal (Refereed) Epub ahead of print
Abstract [en]

A psittacosis epidemic linked to fulmar hunting occurred on the Faroe Islands in the 1930s. This study investigates a plausible explanation to the 20% human mortality in this outbreak. Phylogenetic analysis showed that C. psittaci isolated from fulmars were closely related to the highly virulent 6BC strains from psittacines and is compatible with an acquisition by fulmars of an ancestor of the 6BC clade in the 1930s. This supports the hypothesis that the outbreak on the Faroe Islands started after naïve fulmars acquired C. psittaci from infected dead parrots thrown overboard when shipped to Europe in the 1930s.

National Category
Infectious Medicine
Identifiers
urn:nbn:se:uu:diva-406266 (URN)10.1016/j.micinf.2020.02.007 (DOI)
Available from: 2020-03-12 Created: 2020-03-12 Last updated: 2020-03-17Bibliographically approved
Harding-Esch, E. M., Holland, M. J., Schemann, J.-F., Sillah, A., Sarr, B., Christerson, L., . . . Bailey, R. L. (2019). Impact of a single round of mass drug administration with azithromycin on active trachoma and ocular Chlamydia trachomatis prevalence and circulating strains in The Gambia and Senegal. Parasites & Vectors, 12, Article ID 497.
Open this publication in new window or tab >>Impact of a single round of mass drug administration with azithromycin on active trachoma and ocular Chlamydia trachomatis prevalence and circulating strains in The Gambia and Senegal
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2019 (English)In: Parasites & Vectors, ISSN 1756-3305, E-ISSN 1756-3305, Vol. 12, article id 497Article in journal (Refereed) Published
Abstract [en]

Background: Mass drug administration (MDA) with azithromycin is a cornerstone of the trachoma elimination strategy. Although the global prevalence of active trachoma has declined considerably, prevalence persists or even increases in some communities and districts. To increase understanding of MDA impact, we investigated the prevalence of active trachoma and ocular C. trachomatis prevalence, organism load, and circulating strains at baseline and one-year post-MDA in The Gambia and Senegal.

Methods: Pre- and one-year post-MDA, children aged 0-9 years were examined for clinical signs of trachoma in six Gambian and 12 Senegalese villages. Ocular swabs from each child's right conjunctiva were tested for evidence of ocular C. trachomatis infection and organism load (ompA copy number), and ompA and multi-locus sequence typing (MLST) was performed.

Results: A total of 1171 children were examined at baseline and follow-up in The Gambia. Active trachoma prevalence decreased from 23.9% to 17.7%, whereas ocular C. trachomatis prevalence increased from 3.0% to 3.8%. In Senegal, 1613 and 1771 children were examined at baseline and follow-up, respectively. Active trachoma prevalence decreased from 14.9% to 8.0%, whereas ocular C. trachomatis prevalence increased from 1.8% to 3.6%. Higher organism load was associated with having active trachoma and severe inflammation. Sequence typing demonstrated that all Senegalese samples were genovar A, whereas Gambian samples were a mix of genovars A and B. MLST provided evidence of clustering at village and household levels and demonstrated differences of strain variant frequencies in Senegal, indicative of an "outbreak". MLST, including partial ompA typing, provided greater discriminatory power than complete ompA typing.

Conclusions: We found that one round of MDA led to an overall decline in active trachoma prevalence but no impact on ocular C. trachomatis infection, with heterogeneity observed between villages studied. This could not be explained by MDA coverage or number of different circulating strains pre- and post-MDA. The poor correlation between active trachoma and infection prevalence supports the need for further work on alternative indicators to clinical signs for diagnosing ocular C. trachomatis infection. MLST typing has potential molecular epidemiology utility, including better understanding of transmission dynamics, although relationship to whole-genome sequence variability requires further exploration.

Place, publisher, year, edition, pages
BMC, 2019
Keywords
Active trachoma, Chlamydia trachomatis, Ocular, Mass drug administration, Azithromycin, Prevalence, Whole-genome sequence, Organism load, ompA, Multi locus sequence typing
National Category
Microbiology
Identifiers
urn:nbn:se:uu:diva-396966 (URN)10.1186/s13071-019-3743-x (DOI)000491904100004 ()31640755 (PubMedID)
Funder
Wellcome trust, 078460/Z/05/Z
Available from: 2019-11-13 Created: 2019-11-13 Last updated: 2019-11-13Bibliographically approved
Goncalves, O. S., Christiansen, G., Holm, A., Herrmann, B., Klintstedt, M., Petersen, S. B. & Birkelund, S. (2019). The repeated 36 amino acid motif of Chlamydia trachomatis Hc2 protein binds to the major groove of DNA. Research in Microbiology, 170(6-7), 256-262
Open this publication in new window or tab >>The repeated 36 amino acid motif of Chlamydia trachomatis Hc2 protein binds to the major groove of DNA
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2019 (English)In: Research in Microbiology, ISSN 0923-2508, E-ISSN 1769-7123, Vol. 170, no 6-7, p. 256-262Article in journal (Refereed) Published
Abstract [en]

The gram-negative, obligate intracellular human pathogen, Chlamydia trachomatis has a bi-phasic developmental cycle. The histone H1-like C. trachomatis DNA binding protein, Hc2, is produced late during the developmental cycle when the dividing reticulate body transforms into the smaller, metabolically inactive elementary body. Together with Hc1, the two proteins compact the chlamydial chromosome and arrest replication and transcription. Hc2 is heterogeneous in length due to variation in the number of lysine rich pentamers. Six pentamers and one hexamer constitute a 36 amino acid long repetitive unit that, in spite of variations, is unique for Chlamydiaceae. Using synthetic peptides, the DNA-binding capacity of the 36 amino acid peptide and that of a randomized peptide was analyzed. Both peptides bound and compacted plasmid DNA, however, electron microscopy of peptide/DNA complexes showed major differences in the resulting aggregated structures. Fluorescence spectroscopy was used to analyze the binding. After complexing plasmid DNA with each of three different intercalating dyes, increasing amounts of peptides were added and fluorescence spectroscopy performed. The major groove binder, methyl green, was displaced by both peptides at low concentrations, while the minor groove binder, Hoechts, and the intercalating dye, Ethidium Bromide, were displaced only at high concentrations of peptides. (C) 2019 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Place, publisher, year, edition, pages
ELSEVIER, 2019
Keywords
Chlamydia trachomatis, Histone H1-like protein, Hc2, DNA packing, Fluorescence spectroscopy, Methyl green
National Category
Physical Chemistry
Identifiers
urn:nbn:se:uu:diva-396620 (URN)10.1016/j.resmic.2019.08.002 (DOI)000491121700002 ()31419583 (PubMedID)
Available from: 2019-11-13 Created: 2019-11-13 Last updated: 2019-11-13Bibliographically approved
Rivas, L., Reuterswärd, P., Rasti, R., Herrmann, B., Mårtensson, A., Alfven, T., . . . Andersson-Svahn, H. (2018). A vertical flow paper-microarray assay with isothermal DNA amplification for detection of Neisseria meningitidis. Talanta: The International Journal of Pure and Applied Analytical Chemistry, 183, 192-200
Open this publication in new window or tab >>A vertical flow paper-microarray assay with isothermal DNA amplification for detection of Neisseria meningitidis
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2018 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 183, p. 192-200Article in journal (Refereed) Published
Abstract [en]

Paper-based biosensors offer a promising technology to be used at the point of care, enabled by good performance, convenience and low-cost. In this article, we describe a colorimetric vertical-flow DNA microarray (DNAVFM) that takes advantage of the screening capability of DNA microarrays in a paper format together with isothermal amplification by means of Recombinase Polymerase Amplification (RPA). Different assay parameters such as hybridization buffer, flow rate, printing buffer and capture probe concentration were optimized. A limit of detection (LOD) of 4.4 nM was achieved as determined by tabletop scanning. The DNA-VFM was applied as a proof of concept for detection of Neisseria meningitidis, a primary cause of bacterial meningitis. The LOD was determined to be between 38 and 2.1 x 10(6) copies/VFMassay, depending on the choice of DNA capture probes. The presented approach provides multiplex capabilities of DNA microarrays in a paper-based format for future point-of-care applications.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV, 2018
Keywords
Microarray, Paper-based biosensor, Point-of-care, Gold nanoparticles, Neisseria meningitidis
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-356185 (URN)10.1016/j.talanta.2018.02.070 (DOI)000430645800026 ()29567164 (PubMedID)
Funder
Swedish Research CouncilEU, European Research Council, 615458
Available from: 2018-08-02 Created: 2018-08-02 Last updated: 2018-08-02Bibliographically approved
Pineiro, L., Isaksson, J., Zapico, M., Cilla, G. & Herrmann, B. (2018). Chlamydia trachomatis genotypes A and B from urogenital specimens of patients in Spain: molecular characterization. Clinical Microbiology and Infection, 24(8), 910.e5-910.e8
Open this publication in new window or tab >>Chlamydia trachomatis genotypes A and B from urogenital specimens of patients in Spain: molecular characterization
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2018 (English)In: Clinical Microbiology and Infection, ISSN 1198-743X, E-ISSN 1469-0691, Vol. 24, no 8, p. 910.e5-910.e8Article in journal (Refereed) Published
Abstract [en]

Objectives: Chlamydia trachomatis ompA genotypes A and B, primarily associated with trachoma, were unexpectedly detected in urogenital samples of patients in Spain, a trachoma-free country. In this study, we aimed to explain this finding using analysis of organotropism-related genes and a multilocus sequence typing (MLST) technique.

Methods: C trachomatis genotypes A or B were detected in 8/930 (0.9%) infection episodes between 2006 and 2012. In these strains, organotropism-related genes (polymorphic membrane protein gene H, tryptophan synthase gene A, CTA0934, and cytotoxin) were studied. Further, the strains were analysed by MLST, using a polymerase chain reaction that amplifies five highly variable genomic loci (hctB, CT058, CT144, CT172, and pbpB). Amplicons were sequenced and phylogenetic analysis was conducted.

Results: Seven strains were detected in the eight infection episodes (in one patient, an identical strain being found in two episodes). Analysis of organotropism-related genes showed that these strains shared genetic features characteristic of genitotropic genotypes but not of trachoma strains. Three strains of genotype A showed a unique and new MLST-sequence type (ST551, allele profile 8-8-2-27-69). The four strains of genotype B belonged to ST138.

Conclusions: C. trachomatis ompA genotypes A and B associated with trachoma, but detected sporadically in urogenital samples in trachoma-free countries, may be the result of recombination between strains adapted to trachoma and strains adapted to sexual transmission.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD, 2018
Keywords
Chlamydia trachomatis, Multilocus sequence typing, ompA genotypes A and B, Organotropic genes, Urogenital samples
National Category
Microbiology in the medical area Microbiology
Identifiers
urn:nbn:se:uu:diva-361495 (URN)10.1016/j.cmi.2018.01.025 (DOI)000439403900024 ()29427803 (PubMedID)
Available from: 2018-09-26 Created: 2018-09-26 Last updated: 2018-09-26Bibliographically approved
Dahlberg, J., Hadad, R., Elfving, K., Larsson, I., Isaksson, J., Magnuson, A., . . . Herrmann, B. (2018). Ten years transmission of the new variant of Chlamydia trachomatis in Sweden: prevalence of infections and associated complications.. Sexually Transmitted Infections, 94(2), 100-104
Open this publication in new window or tab >>Ten years transmission of the new variant of Chlamydia trachomatis in Sweden: prevalence of infections and associated complications.
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2018 (English)In: Sexually Transmitted Infections, ISSN 1368-4973, E-ISSN 1472-3263, Vol. 94, no 2, p. 100-104Article in journal (Refereed) Published
Abstract [en]

OBJECTIVES: (nvCT) was discovered in Sweden. It has a deletion in the plasmid resulting in failed detection by the single target systems from Abbott and Roche used at that time, whereas the third system used, from Becton Dickinson (BD), detects nvCT. The proportion of nvCT was initially up to 65% in counties using Abbott/Roche systems. This study analysed the proportion of nvCT from 2007 to 2015 in four selected counties and its impact on chlamydia-associated complications.

METHODS: sequencing. Ectopic pregnancy and pelvic inflammatory disease records were extracted from the national registers.

RESULTS: -positive samples were analysed. The nvCT proportion significantly decreased in the two counties using Roche systems, from 56% in 2007 to 6.5% in 2015 (p<0.001). In the two counties using BD systems, a decrease was also seen, from 19% in 2007 to 5.2% in 2015 (p<0.001). Fifteen nvCT cases from 2015 and 102 cases from 2006 to 2009 had identical MLST profiles. Counties using Roche/Abbott systems showed higher mean rates of ectopic pregnancy and pelvic inflammatory disease compared with counties using BD systems.

CONCLUSIONS: The nvCT proportion has decreased in all counties and converged to a low prevalence irrespective of previous rates. Genotyping showed that nvCT is clonal and genetically stable. Failing detection only marginally affected complication rates.

Keywords
chlamydia trachomatis, ectopic pregnancy, epidemiology, pelvic inflammatory disease, plasmid
National Category
Clinical Laboratory Medicine
Identifiers
urn:nbn:se:uu:diva-343605 (URN)10.1136/sextrans-2016-052992 (DOI)000428207800007 ()28724744 (PubMedID)
Available from: 2018-02-28 Created: 2018-02-28 Last updated: 2018-07-23Bibliographically approved
Herrmann, B., Isaksson, J., Carlsson, O., Airell, Å., Strömdahl, S. & Bratt, G. (2017). LYMPHOGRANULOMA VENEREUM IN SWEDEN 2004-2016: INCREASED RATES AMONG HIV-NEGATIVE MEN WHO HAVE SEX WITH MEN AND CHANGED GENOTYPES. Sexually Transmitted Infections, 93(Suppl. 2), A103-A103, Article ID P3.27.
Open this publication in new window or tab >>LYMPHOGRANULOMA VENEREUM IN SWEDEN 2004-2016: INCREASED RATES AMONG HIV-NEGATIVE MEN WHO HAVE SEX WITH MEN AND CHANGED GENOTYPES
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2017 (English)In: Sexually Transmitted Infections, ISSN 1368-4973, E-ISSN 1472-3263, Vol. 93, no Suppl. 2, p. A103-A103, article id P3.27Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
BMJ PUBLISHING GROUP, 2017
National Category
Dermatology and Venereal Diseases
Identifiers
urn:nbn:se:uu:diva-377410 (URN)10.1136/sextrans-2017-053264.264 (DOI)000442492000265 ()
Available from: 2019-02-20 Created: 2019-02-20 Last updated: 2019-02-20Bibliographically approved
Isaksson, J., Carlsson, O., Airell, A., Strömdahl, S., Bratt, G. & Herrmann, B. (2017). Lymphogranuloma venereum rates increased and Chlamydia trachomatis genotypes changed among men who have sex with men in Sweden 2004-2016. Journal of Medical Microbiology, 66(11), 1684-1687
Open this publication in new window or tab >>Lymphogranuloma venereum rates increased and Chlamydia trachomatis genotypes changed among men who have sex with men in Sweden 2004-2016
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2017 (English)In: Journal of Medical Microbiology, ISSN 0022-2615, E-ISSN 1473-5644, Vol. 66, no 11, p. 1684-1687Article in journal (Refereed) Published
Abstract [en]

This study aimed to determine the incidence of lymphogranuloma venereum (LGV) in Sweden since 2004 and to study in detail a consecutive number of Chlamydia trachomatis cases in men who have sex with men (MSM) during a 10 month period (September 2014 to July 2015). LGV increased from sporadic import cases in 2004 to comprise a spread within Sweden in 2016. Initially, only the L2b ompA genotype was detected, but in 2015 half of the genotyped LGV cases were L2 genotype. The changing genotype distribution in Sweden is linked to increased LGV spread in Europe. High-resolution multilocus sequence typing of 168 C. trachomatis cases from MSM in 2015 resulted in 29 sequence types, of which 3 accounted for 49% of cases. The increased rates and different genotypes of LGV indicate that more concern for high-risk taking MSM is needed to avoid further spread of this invasive infection.

Keywords
Chlamydia trachomatis, lymphogranuloma venereum, men who have sex with men (MSM), genotyping, ompA, multilocus sequence typing (MLST)
National Category
Infectious Medicine Microbiology in the medical area Public Health, Global Health, Social Medicine and Epidemiology
Identifiers
urn:nbn:se:uu:diva-341657 (URN)10.1099/jmm.0.000597 (DOI)000414369800023 ()29022544 (PubMedID)
Funder
Public Health Agency of Sweden , 821/2013
Available from: 2018-02-16 Created: 2018-02-16 Last updated: 2018-02-16Bibliographically approved
Abdeldaim, G., Svensson, E., Blomberg, J. & Herrmann, B. (2016). Duplex detection of the Mycobacterium tuberculosis complex and medically important non-tuberculosis mycobacteria by real-time PCR based on the rnpB gene. Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), 124(11), 991-995
Open this publication in new window or tab >>Duplex detection of the Mycobacterium tuberculosis complex and medically important non-tuberculosis mycobacteria by real-time PCR based on the rnpB gene
2016 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 124, no 11, p. 991-995Article in journal (Refereed) Published
Abstract [en]

A duplex real-time PCR based on the rnpB gene was developed for Mycobacterium spp. The assay was specific for the Mycobacterium tuberculosis complex (MTB) and also detected all 19 tested species of non-tuberculous mycobacteria (NTM). The assay was evaluated on 404 clinical samples: 290 respiratory samples and 114 from tissue and other nonrespiratory body sites. M. tuberculosis was detected by culture in 40 samples and in 30 samples by the assay. The MTB assay showed a sensitivity similar to Roche Cobas Amplicor MTB-PCR (Roche Molecular Systems, Pleasanton, CA, USA). There were only nine samples with non-tuberculous mycobacteria detected by culture. Six of them were detected by the PCR assay.

Keywords
Mycobacterium tuberculosis, non-tuberculosis mycobacteria, real-time PCR, rnpB
National Category
Immunology in the medical area
Identifiers
urn:nbn:se:uu:diva-311199 (URN)10.1111/apm.12598 (DOI)000388264900011 ()27677426 (PubMedID)
Available from: 2016-12-22 Created: 2016-12-22 Last updated: 2018-01-13Bibliographically approved
Isaksson, J., Christerson, L., Blomqvist, M., Wille, M., Alladio, L. A., Sachse, K., . . . Herrmann, B. (2015). Chlamydiaceae-like bacterium, but no Chlamydia psittaci, in sea birds from Antarctica. Polar Biology, 38(11), 1931-1936
Open this publication in new window or tab >>Chlamydiaceae-like bacterium, but no Chlamydia psittaci, in sea birds from Antarctica
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2015 (English)In: Polar Biology, ISSN 0722-4060, E-ISSN 1432-2056, Vol. 38, no 11, p. 1931-1936Article in journal (Refereed) Published
Abstract [en]

Within the growing order of Chlamydiales, there are a number of pathogens. One is Chlamydia psittaci, a zoonotic pathogen, with birds as natural hosts that may be transmitted to humans and cause severe respiratory disease, psittacosis. The prevalence of this pathogen in Antarctic birds is almost unknown as well as the ramifications of its potential spread in naïve bird populations. To investigate the prevalence of chlamydia organisms, cloacal and fecal samples were collected from 264 penguins and 263 seabirds on the Antarctic Peninsula and in Southern Chile. No C. psittaci could be detected by 23S rRNA real-time PCR. However, DNA sequencing of the 16S rRNA 298-bp signature sequence revealed a Chlamydiaceae-like bacterium previously found in seabirds from the subarctic zone, demonstrating that this not yet fully characterized bacterium is widespread. In conclusion, the prevalence of C. psittaci among wild birds on the Antarctic Peninsula seems to be low, but other types of chlamydial organisms are common. Further studies are required to taxonomically define and finally understand the role of these non-classified Chlamydiae.

Keywords
Chlamydia psittaci; Chlamydiaceae; Pygoscelis antarcticus; Spheniscus magellanicus; Stercorarius antarcticus; Antarctic
National Category
Infectious Medicine
Identifiers
urn:nbn:se:uu:diva-265076 (URN)10.1007/s00300-015-1748-2 (DOI)000364023800012 ()
Available from: 2015-10-21 Created: 2015-10-21 Last updated: 2017-12-01Bibliographically approved
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