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Herrmann, Björn
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Publications (10 of 68) Show all publications
Rivas, L., Reuterswärd, P., Rasti, R., Herrmann, B., Mårtensson, A., Alfven, T., . . . Andersson-Svahn, H. (2018). A vertical flow paper-microarray assay with isothermal DNA amplification for detection of Neisseria meningitidis. Talanta: The International Journal of Pure and Applied Analytical Chemistry, 183, 192-200
Open this publication in new window or tab >>A vertical flow paper-microarray assay with isothermal DNA amplification for detection of Neisseria meningitidis
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2018 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 183, p. 192-200Article in journal (Refereed) Published
Abstract [en]

Paper-based biosensors offer a promising technology to be used at the point of care, enabled by good performance, convenience and low-cost. In this article, we describe a colorimetric vertical-flow DNA microarray (DNAVFM) that takes advantage of the screening capability of DNA microarrays in a paper format together with isothermal amplification by means of Recombinase Polymerase Amplification (RPA). Different assay parameters such as hybridization buffer, flow rate, printing buffer and capture probe concentration were optimized. A limit of detection (LOD) of 4.4 nM was achieved as determined by tabletop scanning. The DNA-VFM was applied as a proof of concept for detection of Neisseria meningitidis, a primary cause of bacterial meningitis. The LOD was determined to be between 38 and 2.1 x 10(6) copies/VFMassay, depending on the choice of DNA capture probes. The presented approach provides multiplex capabilities of DNA microarrays in a paper-based format for future point-of-care applications.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV, 2018
Keywords
Microarray, Paper-based biosensor, Point-of-care, Gold nanoparticles, Neisseria meningitidis
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-356185 (URN)10.1016/j.talanta.2018.02.070 (DOI)000430645800026 ()29567164 (PubMedID)
Funder
Swedish Research CouncilEU, European Research Council, 615458
Available from: 2018-08-02 Created: 2018-08-02 Last updated: 2018-08-02Bibliographically approved
Pineiro, L., Isaksson, J., Zapico, M., Cilla, G. & Herrmann, B. (2018). Chlamydia trachomatis genotypes A and B from urogenital specimens of patients in Spain: molecular characterization. Clinical Microbiology and Infection, 24(8), 910.e5-910.e8
Open this publication in new window or tab >>Chlamydia trachomatis genotypes A and B from urogenital specimens of patients in Spain: molecular characterization
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2018 (English)In: Clinical Microbiology and Infection, ISSN 1198-743X, E-ISSN 1469-0691, Vol. 24, no 8, p. 910.e5-910.e8Article in journal (Refereed) Published
Abstract [en]

Objectives: Chlamydia trachomatis ompA genotypes A and B, primarily associated with trachoma, were unexpectedly detected in urogenital samples of patients in Spain, a trachoma-free country. In this study, we aimed to explain this finding using analysis of organotropism-related genes and a multilocus sequence typing (MLST) technique.

Methods: C trachomatis genotypes A or B were detected in 8/930 (0.9%) infection episodes between 2006 and 2012. In these strains, organotropism-related genes (polymorphic membrane protein gene H, tryptophan synthase gene A, CTA0934, and cytotoxin) were studied. Further, the strains were analysed by MLST, using a polymerase chain reaction that amplifies five highly variable genomic loci (hctB, CT058, CT144, CT172, and pbpB). Amplicons were sequenced and phylogenetic analysis was conducted.

Results: Seven strains were detected in the eight infection episodes (in one patient, an identical strain being found in two episodes). Analysis of organotropism-related genes showed that these strains shared genetic features characteristic of genitotropic genotypes but not of trachoma strains. Three strains of genotype A showed a unique and new MLST-sequence type (ST551, allele profile 8-8-2-27-69). The four strains of genotype B belonged to ST138.

Conclusions: C. trachomatis ompA genotypes A and B associated with trachoma, but detected sporadically in urogenital samples in trachoma-free countries, may be the result of recombination between strains adapted to trachoma and strains adapted to sexual transmission.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD, 2018
Keywords
Chlamydia trachomatis, Multilocus sequence typing, ompA genotypes A and B, Organotropic genes, Urogenital samples
National Category
Microbiology in the medical area Microbiology
Identifiers
urn:nbn:se:uu:diva-361495 (URN)10.1016/j.cmi.2018.01.025 (DOI)000439403900024 ()29427803 (PubMedID)
Available from: 2018-09-26 Created: 2018-09-26 Last updated: 2018-09-26Bibliographically approved
Dahlberg, J., Hadad, R., Elfving, K., Larsson, I., Isaksson, J., Magnuson, A., . . . Herrmann, B. (2018). Ten years transmission of the new variant of Chlamydia trachomatis in Sweden: prevalence of infections and associated complications.. Sexually Transmitted Infections, 94(2), 100-104
Open this publication in new window or tab >>Ten years transmission of the new variant of Chlamydia trachomatis in Sweden: prevalence of infections and associated complications.
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2018 (English)In: Sexually Transmitted Infections, ISSN 1368-4973, E-ISSN 1472-3263, Vol. 94, no 2, p. 100-104Article in journal (Refereed) Published
Abstract [en]

OBJECTIVES: (nvCT) was discovered in Sweden. It has a deletion in the plasmid resulting in failed detection by the single target systems from Abbott and Roche used at that time, whereas the third system used, from Becton Dickinson (BD), detects nvCT. The proportion of nvCT was initially up to 65% in counties using Abbott/Roche systems. This study analysed the proportion of nvCT from 2007 to 2015 in four selected counties and its impact on chlamydia-associated complications.

METHODS: sequencing. Ectopic pregnancy and pelvic inflammatory disease records were extracted from the national registers.

RESULTS: -positive samples were analysed. The nvCT proportion significantly decreased in the two counties using Roche systems, from 56% in 2007 to 6.5% in 2015 (p<0.001). In the two counties using BD systems, a decrease was also seen, from 19% in 2007 to 5.2% in 2015 (p<0.001). Fifteen nvCT cases from 2015 and 102 cases from 2006 to 2009 had identical MLST profiles. Counties using Roche/Abbott systems showed higher mean rates of ectopic pregnancy and pelvic inflammatory disease compared with counties using BD systems.

CONCLUSIONS: The nvCT proportion has decreased in all counties and converged to a low prevalence irrespective of previous rates. Genotyping showed that nvCT is clonal and genetically stable. Failing detection only marginally affected complication rates.

Keywords
chlamydia trachomatis, ectopic pregnancy, epidemiology, pelvic inflammatory disease, plasmid
National Category
Clinical Laboratory Medicine
Identifiers
urn:nbn:se:uu:diva-343605 (URN)10.1136/sextrans-2016-052992 (DOI)000428207800007 ()28724744 (PubMedID)
Available from: 2018-02-28 Created: 2018-02-28 Last updated: 2018-07-23Bibliographically approved
Herrmann, B., Isaksson, J., Carlsson, O., Airell, Å., Strömdahl, S. & Bratt, G. (2017). LYMPHOGRANULOMA VENEREUM IN SWEDEN 2004-2016: INCREASED RATES AMONG HIV-NEGATIVE MEN WHO HAVE SEX WITH MEN AND CHANGED GENOTYPES. Sexually Transmitted Infections, 93(Suppl. 2), A103-A103, Article ID P3.27.
Open this publication in new window or tab >>LYMPHOGRANULOMA VENEREUM IN SWEDEN 2004-2016: INCREASED RATES AMONG HIV-NEGATIVE MEN WHO HAVE SEX WITH MEN AND CHANGED GENOTYPES
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2017 (English)In: Sexually Transmitted Infections, ISSN 1368-4973, E-ISSN 1472-3263, Vol. 93, no Suppl. 2, p. A103-A103, article id P3.27Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
BMJ PUBLISHING GROUP, 2017
National Category
Dermatology and Venereal Diseases
Identifiers
urn:nbn:se:uu:diva-377410 (URN)10.1136/sextrans-2017-053264.264 (DOI)000442492000265 ()
Available from: 2019-02-20 Created: 2019-02-20 Last updated: 2019-02-20Bibliographically approved
Isaksson, J., Carlsson, O., Airell, A., Strömdahl, S., Bratt, G. & Herrmann, B. (2017). Lymphogranuloma venereum rates increased and Chlamydia trachomatis genotypes changed among men who have sex with men in Sweden 2004-2016. Journal of Medical Microbiology, 66(11), 1684-1687
Open this publication in new window or tab >>Lymphogranuloma venereum rates increased and Chlamydia trachomatis genotypes changed among men who have sex with men in Sweden 2004-2016
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2017 (English)In: Journal of Medical Microbiology, ISSN 0022-2615, E-ISSN 1473-5644, Vol. 66, no 11, p. 1684-1687Article in journal (Refereed) Published
Abstract [en]

This study aimed to determine the incidence of lymphogranuloma venereum (LGV) in Sweden since 2004 and to study in detail a consecutive number of Chlamydia trachomatis cases in men who have sex with men (MSM) during a 10 month period (September 2014 to July 2015). LGV increased from sporadic import cases in 2004 to comprise a spread within Sweden in 2016. Initially, only the L2b ompA genotype was detected, but in 2015 half of the genotyped LGV cases were L2 genotype. The changing genotype distribution in Sweden is linked to increased LGV spread in Europe. High-resolution multilocus sequence typing of 168 C. trachomatis cases from MSM in 2015 resulted in 29 sequence types, of which 3 accounted for 49% of cases. The increased rates and different genotypes of LGV indicate that more concern for high-risk taking MSM is needed to avoid further spread of this invasive infection.

Keywords
Chlamydia trachomatis, lymphogranuloma venereum, men who have sex with men (MSM), genotyping, ompA, multilocus sequence typing (MLST)
National Category
Infectious Medicine Microbiology in the medical area Public Health, Global Health, Social Medicine and Epidemiology
Identifiers
urn:nbn:se:uu:diva-341657 (URN)10.1099/jmm.0.000597 (DOI)000414369800023 ()29022544 (PubMedID)
Funder
Public Health Agency of Sweden , 821/2013
Available from: 2018-02-16 Created: 2018-02-16 Last updated: 2018-02-16Bibliographically approved
Abdeldaim, G., Svensson, E., Blomberg, J. & Herrmann, B. (2016). Duplex detection of the Mycobacterium tuberculosis complex and medically important non-tuberculosis mycobacteria by real-time PCR based on the rnpB gene. Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), 124(11), 991-995
Open this publication in new window or tab >>Duplex detection of the Mycobacterium tuberculosis complex and medically important non-tuberculosis mycobacteria by real-time PCR based on the rnpB gene
2016 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 124, no 11, p. 991-995Article in journal (Refereed) Published
Abstract [en]

A duplex real-time PCR based on the rnpB gene was developed for Mycobacterium spp. The assay was specific for the Mycobacterium tuberculosis complex (MTB) and also detected all 19 tested species of non-tuberculous mycobacteria (NTM). The assay was evaluated on 404 clinical samples: 290 respiratory samples and 114 from tissue and other nonrespiratory body sites. M. tuberculosis was detected by culture in 40 samples and in 30 samples by the assay. The MTB assay showed a sensitivity similar to Roche Cobas Amplicor MTB-PCR (Roche Molecular Systems, Pleasanton, CA, USA). There were only nine samples with non-tuberculous mycobacteria detected by culture. Six of them were detected by the PCR assay.

Keywords
Mycobacterium tuberculosis, non-tuberculosis mycobacteria, real-time PCR, rnpB
National Category
Immunology in the medical area
Identifiers
urn:nbn:se:uu:diva-311199 (URN)10.1111/apm.12598 (DOI)000388264900011 ()27677426 (PubMedID)
Available from: 2016-12-22 Created: 2016-12-22 Last updated: 2018-01-13Bibliographically approved
Isaksson, J., Christerson, L., Blomqvist, M., Wille, M., Alladio, L. A., Sachse, K., . . . Herrmann, B. (2015). Chlamydiaceae-like bacterium, but no Chlamydia psittaci, in sea birds from Antarctica. Polar Biology, 38(11), 1931-1936
Open this publication in new window or tab >>Chlamydiaceae-like bacterium, but no Chlamydia psittaci, in sea birds from Antarctica
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2015 (English)In: Polar Biology, ISSN 0722-4060, E-ISSN 1432-2056, Vol. 38, no 11, p. 1931-1936Article in journal (Refereed) Published
Abstract [en]

Within the growing order of Chlamydiales, there are a number of pathogens. One is Chlamydia psittaci, a zoonotic pathogen, with birds as natural hosts that may be transmitted to humans and cause severe respiratory disease, psittacosis. The prevalence of this pathogen in Antarctic birds is almost unknown as well as the ramifications of its potential spread in naïve bird populations. To investigate the prevalence of chlamydia organisms, cloacal and fecal samples were collected from 264 penguins and 263 seabirds on the Antarctic Peninsula and in Southern Chile. No C. psittaci could be detected by 23S rRNA real-time PCR. However, DNA sequencing of the 16S rRNA 298-bp signature sequence revealed a Chlamydiaceae-like bacterium previously found in seabirds from the subarctic zone, demonstrating that this not yet fully characterized bacterium is widespread. In conclusion, the prevalence of C. psittaci among wild birds on the Antarctic Peninsula seems to be low, but other types of chlamydial organisms are common. Further studies are required to taxonomically define and finally understand the role of these non-classified Chlamydiae.

Keywords
Chlamydia psittaci; Chlamydiaceae; Pygoscelis antarcticus; Spheniscus magellanicus; Stercorarius antarcticus; Antarctic
National Category
Infectious Medicine
Identifiers
urn:nbn:se:uu:diva-265076 (URN)10.1007/s00300-015-1748-2 (DOI)000364023800012 ()
Available from: 2015-10-21 Created: 2015-10-21 Last updated: 2017-12-01Bibliographically approved
Alpkvist, H., Athlin, S., Naucler, P., Herrmann, B., Abdeldaim, G., Slotved, H.-C., . . . Stralin, K. (2015). Clinical and Microbiological Factors Associated with High Nasopharyngeal Pneumococcal Density in Patients with Pneumococcal Pneumonia. PLoS ONE, 10(10), Article ID e0140112.
Open this publication in new window or tab >>Clinical and Microbiological Factors Associated with High Nasopharyngeal Pneumococcal Density in Patients with Pneumococcal Pneumonia
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2015 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 10, no 10, article id e0140112Article in journal (Refereed) Published
Abstract [en]

Background We aimed to study if certain clinical and/or microbiological factors are associated with a high nasopharyngeal (NP) density of Streptococcus pneumoniae in pneumococcal pneumonia. In addition, we aimed to study if a high NP pneumococcal density could be useful to detect severe pneumococcal pneumonia. Methods Adult patients hospitalized for radiologically confirmed community-acquired pneumonia were included in a prospective study. NP aspirates were collected at admission and were subjected to quantitative PCR for pneumococcal DNA (Spn9802 DNA). Patients were considered to have pneumococcal etiology if S. pneumoniae was detected in blood culture and/ or culture of respiratory secretions and/or urinary antigen test. Results Of 166 included patients, 68 patients had pneumococcal DNA detected in NP aspirate. Pneumococcal etiology was noted in 57 patients (84%) with positive and 8 patients (8.2%) with negative test for pneumococcal DNA (p<0.0001). The median NP pneumococcal density of DNA positive patients with pneumococcal etiology was 6.83 log(10) DNA copies/mL (range 1.79-9.50). In a multivariate analysis of patients with pneumococcal etiology, a high pneumococcal density was independently associated with severe pneumonia (Pneumonia Severity Index risk class IV-V), symptom duration >= 2 days prior to admission, and a medium/high serum immunoglobulin titer against the patient's own pneumococcal serotype. NP pneumococcal density was not associated with sex, age, smoking, co-morbidity, viral co-infection, pneumococcal serotype, or bacteremia. Severe pneumococcal pneumonia was noted in 28 study patients. When we studied the performance of PCR with different DNA cut-off levels for detection of severe pneumococcal pneumonia, we found sensitivities of 54-82% and positive predictive values of 37-56%, indicating suboptimal performance. Conclusions Pneumonia severity, symptom duration similar to 2 days, and a medium/high serum immunoglobulin titer against the patient's own serotype were independently associated with a high NP pneumococcal density. NP pneumococcal density has limited value for detection of severe pneumococcal pneumonia.

National Category
Respiratory Medicine and Allergy Infectious Medicine
Identifiers
urn:nbn:se:uu:diva-267671 (URN)10.1371/journal.pone.0140112 (DOI)000363183100080 ()26466142 (PubMedID)
Available from: 2015-11-25 Created: 2015-11-25 Last updated: 2017-12-01Bibliographically approved
Isaksson, J., Rasmussen, M., Nilson, B., Stadler, L. S., Kurland, S., Olaison, L., . . . Herrmann, B. (2015). Comparison of species identification of endocarditis associated viridans streptococci using rnpB genotyping and 2 MALDI-TOF systems. Diagnostic microbiology and infectious disease, 81(4), 240-245
Open this publication in new window or tab >>Comparison of species identification of endocarditis associated viridans streptococci using rnpB genotyping and 2 MALDI-TOF systems
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2015 (English)In: Diagnostic microbiology and infectious disease, ISSN 0732-8893, E-ISSN 1879-0070, Vol. 81, no 4, p. 240-245Article in journal (Refereed) Published
Abstract [en]

Streptococcus spp. are important causes of infective endocarditis but challenging in species identification. This study compared identification based on sequence determination of the rnpB gene with 2 systems of matrix-assisted laser desorption ionization-time of flight mass spectrometry, MALDI Biotyper (Bruker) and VITEK MS IVD (bioMérieux). Blood culture isolates of viridans streptococci from 63 patients with infective endocarditis were tested. The 3 methods showed full agreement for all 36 isolates identified in the Anginosus, Bovis, and Mutans groups or identified as Streptococcus cristatus, Streptococcus gordonii, or Streptococcus sanguinis. None of the methods could reliably identify the 23 isolates to the species level when designated as Streptococcus mitis, Streptococcus oralis, or Streptococcus tigurinus. In 7 isolates classified to the Mitis group, the rnpB sequences deviated strikingly from all reference sequences, and additional analysis of sodA and groEL genes indicated the occurrence of yet unidentified Streptococcus spp.

National Category
Clinical Medicine
Identifiers
urn:nbn:se:uu:diva-246397 (URN)10.1016/j.diagmicrobio.2014.12.007 (DOI)000352117200003 ()25616316 (PubMedID)
Available from: 2015-03-06 Created: 2015-03-06 Last updated: 2017-12-04Bibliographically approved
Redmond, S. M., Alexander-Kisslig, K., Woodhall, S. C., van den Broek, I. V. F., van Bergen, J., Ward, H., . . . Low, N. (2015). Genital Chlamydia Prevalence in Europe and Non-European High Income Countries: Systematic Review and Meta-Analysis. PLoS ONE, 10(1), Article ID UNSP e0115753.
Open this publication in new window or tab >>Genital Chlamydia Prevalence in Europe and Non-European High Income Countries: Systematic Review and Meta-Analysis
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2015 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 10, no 1, article id UNSP e0115753Article in journal (Refereed) Published
Abstract [en]

Background Accurate information about the prevalence of Chlamydia trachomatis is needed to assess national prevention and control measures. Methods We systematically reviewed population-based cross-sectional studies that estimated chlamydia prevalence in European Union/European Economic Area (EU/EEA) Member States and non-European high income countries from January 1990 to August 2012. We examined results in forest plots, explored heterogeneity using the I-2 statistic, and conducted random effects meta-analysis if appropriate. Meta-regression was used to examine the relationship between study characteristics and chlamydia prevalence estimates. Results We included 25 population-based studies from 11 EU/EEA countries and 14 studies from five other high income countries. Four EU/ EEAMember States reported on nationally representative surveys of sexually experienced adults aged 18-26 years (response rates 52-71%). In women, chlamydia point prevalence estimates ranged from 3.0-5.3%; the pooled average of these estimates was 3.6%(95% CI 2.4, 4.8, I-2 0%). In men, estimates ranged from 2.4-7.3% (pooled average 3.5%; 95% CI 1.9, 5.2, I-2 27%). Estimates in EU/EEA Member States were statistically consistent with those in other high income countries (I-2 0% for women, 6% for men). There was statistical evidence of an association between survey response rate and estimated chlamydia prevalence; estimates were higher in surveys with lower response rates, (p = 0.003 in women, 0.018 in men). Conclusions Population-based surveys that estimate chlamydia prevalence are at risk of participation bias owing to low response rates. Estimates obtained in nationally representative samples of the general population of EU/EEA Member States are similar to estimates from other high income countries.

National Category
Infectious Medicine
Identifiers
urn:nbn:se:uu:diva-302435 (URN)10.1371/journal.pone.0115753 (DOI)000349122100016 ()25615574 (PubMedID)
External cooperation:
Available from: 2016-09-02 Created: 2016-09-02 Last updated: 2017-11-21Bibliographically approved
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