Open this publication in new window or tab >>KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden..
Sci Life Lab, Solna, Sweden.;Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, Stockholm, Sweden..
KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden..
KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden..
Sci Life Lab, Solna, Sweden.;Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, Stockholm, Sweden..
KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden..
KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden..
KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden..
KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden..
Care Sci & Soc, Karolinska Inst, Dept Neurobiol, Stockholm, Sweden..
Karolinska Inst, Dept Med Biochem & Biophys, Div Mol Neurobiol, Stockholm, Sweden..
Royal Inst Technol, Sch Engn Sci Chem, Sci Life Lab, Biotechnol & Hlth, Stockholm, Sweden..
Royal Inst Technol, Sch Engn Sci Chem, Sci Life Lab, Biotechnol & Hlth, Stockholm, Sweden..
Royal Inst Technol, Sch Engn Sci Chem, Sci Life Lab, Biotechnol & Hlth, Stockholm, Sweden..
Sci Life Lab, Solna, Sweden.;Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, Stockholm, Sweden..
Karolinska Inst, Ctr Infect Med, Dept Med Huddinge, Stockholm, Sweden..
Royal Inst Technol, Sch Engn Sci Chem, Sci Life Lab, Biotechnol & Hlth, Stockholm, Sweden..
Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Computerized Image Analysis and Human-Computer Interaction. Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Division Vi3.
Care Sci & Soc, Karolinska Inst, Dept Neurobiol, Stockholm, Sweden..
Karolinska Inst, Dept Med Biochem & Biophys, Div Mol Neurobiol, Stockholm, Sweden..
KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden..
Sci Life Lab, Solna, Sweden.;Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden..
Sci Life Lab, Solna, Sweden.;Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, Stockholm, Sweden.;Justus Liebig Univ, Cardiopulm Inst, Mol Pneumol, Giessen, Germany..
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2023 (English)In: Nature Cell Biology, ISSN 1465-7392, E-ISSN 1476-4679, Vol. 25, no 2, p. 351-365Article in journal (Refereed) Published
Abstract [en]
Sountoulidis et al. provide a spatial gene expression atlas of human embryonic lung during the first trimester of gestation and identify 83 cell identities corresponding to stable cell types or transitional states. The lung contains numerous specialized cell types with distinct roles in tissue function and integrity. To clarify the origins and mechanisms generating cell heterogeneity, we created a comprehensive topographic atlas of early human lung development. Here we report 83 cell states and several spatially resolved developmental trajectories and predict cell interactions within defined tissue niches. We integrated single-cell RNA sequencing and spatially resolved transcriptomics into a web-based, open platform for interactive exploration. We show distinct gene expression programmes, accompanying sequential events of cell differentiation and maturation of the secretory and neuroendocrine cell types in proximal epithelium. We define the origin of airway fibroblasts associated with airway smooth muscle in bronchovascular bundles and describe a trajectory of Schwann cell progenitors to intrinsic parasympathetic neurons controlling bronchoconstriction. Our atlas provides a rich resource for further research and a reference for defining deviations from homeostatic and repair mechanisms leading to pulmonary diseases.
Place, publisher, year, edition, pages
Springer Nature, 2023
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-523259 (URN)10.1038/s41556-022-01064-x (DOI)000916842700001 ()36646791 (PubMedID)
Funder
Knut and Alice Wallenberg Foundation, KAW 2018.0172Swedish Cancer Society, CAN 2018/604Swedish Research Council, 2019-01238Familjen Erling-Perssons StiftelseGerman Research Foundation (DFG), KFO309
2024-02-152024-02-152024-02-15Bibliographically approved