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Hau, Jann
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Publications (10 of 22) Show all publications
Mayo, S., Royo, F. & Hau, J. (2005). Correlation between adjuvanticity and imnunogenicity of cholera toxin B subunit in orally immunised young chickens - Brief report. APMIS, 113(4), 284-287
Open this publication in new window or tab >>Correlation between adjuvanticity and imnunogenicity of cholera toxin B subunit in orally immunised young chickens - Brief report
2005 (English)In: APMIS, Vol. 113, no 4, p. 284-287Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-73752 (URN)
Available from: 2006-03-20 Created: 2006-03-20 Last updated: 2011-01-11
Abelson, K., Adem, B., Royo, F., Carlsson, H.-E. & Hau, J. (2005). High plasma corticosterone levels persist during frequent automatic blood sampling in rats. In Vivo, 19(5), 815-19
Open this publication in new window or tab >>High plasma corticosterone levels persist during frequent automatic blood sampling in rats
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2005 (English)In: In Vivo, ISSN 0258-851X, E-ISSN 1791-7549, Vol. 19, no 5, p. 815-19Article in journal (Refereed) Published
Abstract [en]

Corticosterone levels in blood may be used as a marker of stress in rodents, provided that the blood sampling procedure itself is non-stressful. Automated blood sampling equipment (Accusampler®) allows blood sampling without any interference with the animal and might be useful as a tool for an on-line measurement of stress markers in blood. However, the impact of the blood sampling itself on the corticosterone levels in blood is unknown. The present study was designed to evaluate whether the frequency of blood sampling influences the plasma corticosterone levels in male and female rats. During anaesthesia, a catheter was placed in the jugular vein and attached to an Accusampler®. Blood samples (200 μl) were withdrawn with a high (24 samples) or low frequency (3 samples) during a six-hour period immediately after the catheter insertion. The corticosterone levels in the plasma were quantified with ELISA. The corticosterone levels persisted at high post-operation concentrations when blood was collected frequently, while the levels steadily declined significantly during low-frequency sampling. The corticosterone levels were higher in female than in male rats, but the curves were similar. The present study elucidates the importance of considering the frequency of blood withdrawal during automated blood sampling. This parameter may have an impact on the experimental results when using blood corticosterone levels as a stress marker, but also during any in vivo study where blood is collected, since high corticosterone levels may affect the normal physiology of the animals.

Keywords
rat, stress, corticosterone, Accusampler®, blood sampling, automatic blood sampling
Identifiers
urn:nbn:se:uu:diva-78874 (URN)16097432 (PubMedID)
Available from: 2006-03-31 Created: 2006-03-31 Last updated: 2017-12-14Bibliographically approved
Hau, J. & Coenraad, H. (2005). Refinement of polyclonal antibody production by combining oral immunization of chickens with harvest of antibodies from the egg yolk.. ILAR J, 46(3), 294-299
Open this publication in new window or tab >>Refinement of polyclonal antibody production by combining oral immunization of chickens with harvest of antibodies from the egg yolk.
2005 (English)In: ILAR J, Vol. 46, no 3, p. 294-299Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-73741 (URN)
Available from: 2005-06-16 Created: 2005-06-16 Last updated: 2011-01-11
Mayo, S., Tufvesson, M., Carlsson, H.-E., Royo, F., Gizurarson, S. & Hau, J. (2005). Rhino Vax is an efficient adjuvant in oral immunization of young chickens and cholera toxin B is an effective oral primer in traditional subcutaneous immunization with Freund's incomplete adjuvant. In Vivo, 19, 375-382
Open this publication in new window or tab >>Rhino Vax is an efficient adjuvant in oral immunization of young chickens and cholera toxin B is an effective oral primer in traditional subcutaneous immunization with Freund's incomplete adjuvant
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2005 (English)In: In Vivo, ISSN 0258-851X, E-ISSN 1791-7549, Vol. 19, p. 375-382Article in journal (Refereed) Published
Abstract [en]

Forty-five approximately 50% in-bred 14-day-old White Leghorn female chickens (Gallus domesticus) originating from 11 hens were distributed into 5 treatment groups containing one sister in each treatment group. Phase 1 involved oral administration of an antigen, Bovine Serum Albumin (BSA), in combination with various adjuvant preparations, either Cholera Toxin B-subunit (CTB) and/or RhinoVax® (RV). A positive control group received BSA emulsified in Freund's Incomplete Adjuvant (FIA) by subcutaneous injection. All chickens responded with immunospecific IgA, IgM and IgG antibodies in their circulation. Classical parenteral immunisation with FIA was generally the most potent mode of antigen administration. The highest immunospecific IgG concentrations recorded in the orally-immunised chickens were in the group immunised with 20% RV as the adjuvant. The concentration in this group was approximately 5 times lower than that recorded in the FIA group. For practical egg yolk polyclonal antibody production purposes, the oral regime using 20% RV as adjuvant seems an attractive alternative to the more invasive technique of injecting the antigen in FIA emulsions. In Phase 2 all chickens were subjected to traditional subcutaneous immunisation with a new antigen, human IgG emulsified in FIA. The two groups of chickens that had received CTB orally during Phase 1 responded with significantly higher immunospecific antibody concentrations than did the other chickens, indicating that oral administration of CTB prior to traditional parenteral immunisation may have a priming effect on the humoral immune system. The immunospecific antibody response varied between the 11 families of chickens. There was no correlation between familial responsiveness to oral and subcutaneous immunisations. Families that were high responders to oral immunisation were not high responders to parenteral immunisation and vice versa.

Keywords
adjuvant, animal welfare, chicken, cholera toxin B, Freund's incomplete adjuvan, oral immunisation, RhinoVax®
Identifiers
urn:nbn:se:uu:diva-79155 (URN)15796200 (PubMedID)
Available from: 2006-04-04 Created: 2006-04-04 Last updated: 2017-12-14Bibliographically approved
Abelson, K., Hau, J. & Carlsson, H.-E. (2005). Undergraduate and postgraduate students' responses to mandatory courses (FELASA category C) in laboratory animal science 1997-2003. In: M. R. Gamble (Ed.), Internationalisation and Harmonisation of Laboratory Animal Care and Use Issues: Proceedings of the Ninth FELASA Symposium 14-17 June 2004, Nantes, France.
Open this publication in new window or tab >>Undergraduate and postgraduate students' responses to mandatory courses (FELASA category C) in laboratory animal science 1997-2003
2005 (English)In: Internationalisation and Harmonisation of Laboratory Animal Care and Use Issues: Proceedings of the Ninth FELASA Symposium 14-17 June 2004, Nantes, France / [ed] M. R. Gamble, 2005Conference paper, Published paper (Other academic)
National Category
Biomedical Laboratory Science/Technology
Identifiers
urn:nbn:se:uu:diva-78879 (URN)0-901334-20-0 (ISBN)
Available from: 2006-03-31 Created: 2006-03-31 Last updated: 2010-07-14Bibliographically approved
Bizhanov, G., Jonauskiene, I. & Hau, J. (2004). A novel method, based on lithium sulfate precipitation for purification of chicken egg yolk immunoglobulin Y, applied to immunospecific antibodies against Sendai virus. Scand. J. Lab. Anim. Sci., 31(3), 121-130
Open this publication in new window or tab >>A novel method, based on lithium sulfate precipitation for purification of chicken egg yolk immunoglobulin Y, applied to immunospecific antibodies against Sendai virus
2004 (English)In: Scand. J. Lab. Anim. Sci., Vol. 31, no 3, p. 121-130Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-73753 (URN)
Available from: 2005-06-16 Created: 2005-06-16 Last updated: 2011-01-12
Farah, I., Ngotho, M., Kariuki, T., Jeneby, M., Irura, L., Maina, N., . . . Hau, J. (2004). Animal models for tropical parasitic diseases. In: Handbook in laboratory animal science vol. III: Animal models. : CRC Press, Boca Raton, Florida USA
Open this publication in new window or tab >>Animal models for tropical parasitic diseases
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2004 (English)In: Handbook in laboratory animal science vol. III: Animal models, CRC Press, Boca Raton, Florida USA , 2004Chapter in book (Other scientific)
Place, publisher, year, edition, pages
CRC Press, Boca Raton, Florida USA, 2004
Identifiers
urn:nbn:se:uu:diva-73744 (URN)
Available from: 2005-06-16 Created: 2005-06-16
Hau, J. & van Hoosier, G. (2004). Handbook in laboratory animal science vol. III: Animal models. : CRC Press, Boca Raton, Florida, USA
Open this publication in new window or tab >>Handbook in laboratory animal science vol. III: Animal models
2004 (English)Book (Other scientific)
Place, publisher, year, edition, pages
CRC Press, Boca Raton, Florida, USA, 2004
Identifiers
urn:nbn:se:uu:diva-73745 (URN)
Available from: 2005-06-16 Created: 2005-06-16
Suleman, M., Wango, E., Sapolsky, R., Odongo, H. & Hau, J. (2004). Physiologic Manifestations of Stress from Capture and Restraint of Free-ranging Male African Green Monkeys. Journal of Zoo and Wildlife Medicine, 35, 20
Open this publication in new window or tab >>Physiologic Manifestations of Stress from Capture and Restraint of Free-ranging Male African Green Monkeys
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2004 (English)In: Journal of Zoo and Wildlife Medicine, Vol. 35, p. 20-Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-65997 (URN)
Available from: 2006-09-25 Created: 2006-09-25 Last updated: 2011-01-12
Adencreutz, M. & Hau, J. (2004). Studies of neonatal and juvenile neuromuscular locomotor development of C57BL/6/Bkl, 129SvEv/Bkl and F1 hybrid mice in swim tests. In vivo, 18(6), 733-737
Open this publication in new window or tab >>Studies of neonatal and juvenile neuromuscular locomotor development of C57BL/6/Bkl, 129SvEv/Bkl and F1 hybrid mice in swim tests
2004 (English)In: In vivo, Vol. 18, no 6, p. 733-737Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-73751 (URN)
Available from: 2005-06-16 Created: 2005-06-16 Last updated: 2011-01-12
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