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Nikolajeff, Fredrik
Alternative names
Publications (10 of 94) Show all publications
Sharma, V., Srinivasan, A., Roychoudhury, A., Rani, K., Tyagi, M., Dev, K., . . . Kumar, S. (2019). Characterization of protein extracts from different types of human teeth and insight in biomineralization. Scientific Reports, 9, Article ID 9314.
Open this publication in new window or tab >>Characterization of protein extracts from different types of human teeth and insight in biomineralization
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2019 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, article id 9314Article in journal (Refereed) Published
Abstract [en]

The present study describes an efficient method for isolation and purification of protein extracts from four types of human teeth i.e. molar, premolar, canine, and incisor. Detailed structural characterization of these protein extracts was done by Fourier transform infrared spectroscopy (FTIR) and circular dichroism (CD) which showed that a major fraction of the proteins present are unstructured in nature including primarily random coils in addition to the other structures like extended beta (beta) structure, poly-l-proline-type II (PPII) helix, turns, with only a small fraction constituting of ordered structures like alpha (alpha) helix and beta sheets. These resultant labile structures give the proteins the necessary flexibility that they require to interact with a variety of substrates including different ions like calcium and phosphates and for other protein-protein interactions. We also did initial studies on the mineralization of calcium phosphate with the protein extracts. Nanoparticle tracking analysis (NTA) show an increase in the size of calcium phosphate accumulation in the presence of protein extracts. We propose that protein extracts elevate the crystallization process of calcium phosphate. Our current biophysical study provides novel insights into the structural characterization of proteins from human teeth and their implications in understanding the tooth biomineralization. As per our knowledge, this is the first report which focuses on the whole protein extraction from different types of human teeth as these extracts imitate the in vivo tooth mineralization.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP, 2019
National Category
Dentistry
Identifiers
urn:nbn:se:uu:diva-390817 (URN)10.1038/s41598-019-44268-2 (DOI)000472999700006 ()31249316 (PubMedID)
Funder
Vinnova
Available from: 2019-08-16 Created: 2019-08-16 Last updated: 2019-08-16Bibliographically approved
Qin, T., Qin, W., Ma, M., Zhang, D., Hu, S., Zhang, P., . . . Engqvist, H. (2018). A novel rapid synthesis, characterization and applications of calcium phosphate nanospheres from Baltic seawater. Ceramics International, 44(8), 9076-9079
Open this publication in new window or tab >>A novel rapid synthesis, characterization and applications of calcium phosphate nanospheres from Baltic seawater
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2018 (English)In: Ceramics International, ISSN 0272-8842, E-ISSN 1873-3956, Vol. 44, no 8, p. 9076-9079Article in journal (Refereed) Published
Abstract [en]

Due to the considerable high bio-compatibility, calcium phosphate nanoparticles are widely used in biomedical applications. This study proposes a novel strategy for low-cost manufacturing calcium phosphate nanoporous spheres. The controlled reaction only took less than five minutes, when using Baltic seawater with the dissolved calcium concentration of 2.2 mM as the calcium resources. Porous nanospheres were obtained, with spheres ranging from 50 to 130 nm. The obtained nanospheres possess high drug-loading capacity and exhibit sustained release and pH-dependent properties. In addition, this method provides a general efficient strategy to synthesize other low-cost inorganic nanospheres from seawater.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD, 2018
Keywords
Seawater, Nanosphere, Low cost, Drug, Calcium phosphate
National Category
Materials Chemistry Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-356619 (URN)10.1016/j.ceramint.2018.02.114 (DOI)000430522200044 ()
Available from: 2018-08-20 Created: 2018-08-20 Last updated: 2018-08-23Bibliographically approved
Piron, P., Vargas Catalan, E., Haas, J., Österlund, L., Nikolajeff, F., Andersson, P. O., . . . Karlsson, M. (2018). Development of a diamond waveguide sensor for sensitive protein analysis using IR quantum cascade lasers. In: Yakov G. Soskind (Ed.), SPIE Proceedings Volume 10539: Photonic Instrumentation, Engineering V. Paper presented at SPIE OPTO - Photonics West 2018 - Conference on Photonic Instrumentation Engineering V, San Francisco, CA, USA. January 27-February 1, 2018..
Open this publication in new window or tab >>Development of a diamond waveguide sensor for sensitive protein analysis using IR quantum cascade lasers
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2018 (English)In: SPIE Proceedings Volume 10539: Photonic Instrumentation, Engineering V / [ed] Yakov G. Soskind, 2018Conference paper, Oral presentation with published abstract (Refereed)
National Category
Engineering and Technology Physical Sciences Basic Medicine
Identifiers
urn:nbn:se:uu:diva-340857 (URN)10.1117/12.2289844 (DOI)000432598300012 ()978-1-5106-1564-9 (ISBN)
Conference
SPIE OPTO - Photonics West 2018 - Conference on Photonic Instrumentation Engineering V, San Francisco, CA, USA. January 27-February 1, 2018.
Funder
Carl Tryggers foundation Swedish Research Council, 6212014-5959EU, Horizon 2020, 645758
Available from: 2018-02-04 Created: 2018-02-04 Last updated: 2018-08-30Bibliographically approved
Haas, J., Vargas Catalan, E., Piron, P., Nikolajeff, F., Österlund, L., Karlsson, M. & Mizaikoff, B. (2018). Polycrystalline diamond thin-film waveguides for mid-infrared evanescent field sensors. ACS Omega, 3(6), 6190-6198
Open this publication in new window or tab >>Polycrystalline diamond thin-film waveguides for mid-infrared evanescent field sensors
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2018 (English)In: ACS Omega, ISSN 2470-1343, Vol. 3, no 6, p. 6190-6198Article in journal (Refereed) Published
Abstract [en]

Photonic design and optimization of thin-film polycrystalline diamond waveguides are shown, serving as advanced evanescent field transducers in the mid-infrared fingerprint regime (2000-909 cm(-1); 5-11 mu m). Design constraints inherent to optical/system considerations and the material were implemented in a finite element method (FEM)-based simulation method that allowed three-dimensional modeling of the overall structure. Thus, lateral mode confinement, attenuation in the direction of radiation propagation, and physical resilience were evaluated. In a final step, the designed structures were fabricated, and their utility in combination with a broadly tunable external cavity quantum cascade laser for chemical sensing of a liquid phase analyte was demonstrated.

National Category
Analytical Chemistry Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-346523 (URN)10.1021/acsomega.8b00623 (DOI)000436340500029 ()
Funder
Carl Tryggers foundation Swedish Research CouncilSwedish National Infrastructure for Computing (SNIC), 2016-7-107
Available from: 2018-03-19 Created: 2018-03-19 Last updated: 2018-10-12Bibliographically approved
Qin, T., Ma, M., Qin, W., Xiao, X., Nikolajeff, F. & Engqvist, H. (2018). Rapid precipitation of Mg-doped fluoride-based submicron spheres and evolution study. Journal of Solid State Chemistry (260), 142-146
Open this publication in new window or tab >>Rapid precipitation of Mg-doped fluoride-based submicron spheres and evolution study
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2018 (English)In: Journal of Solid State Chemistry, ISSN 0022-4596, E-ISSN 1095-726X, no 260, p. 142-146Article in journal (Refereed) Published
National Category
Signal Processing
Identifiers
urn:nbn:se:uu:diva-350781 (URN)000428005000021 ()
Available from: 2018-05-16 Created: 2018-05-16 Last updated: 2018-06-04Bibliographically approved
Kumar, S., Srinivasan, A. & Nikolajeff, F. (2018). Role of Infrared Spectroscopy and Imaging in Cancer Diagnosis. Current Medicinal Chemistry, 25(9), 1055-1072
Open this publication in new window or tab >>Role of Infrared Spectroscopy and Imaging in Cancer Diagnosis
2018 (English)In: Current Medicinal Chemistry, ISSN 0929-8673, E-ISSN 1875-533X, Vol. 25, no 9, p. 1055-1072Article, review/survey (Refereed) Published
Abstract [en]

Background: Cancer is a major global health issue. It causes extensive individual suffering and gives a huge burden on the health care in society. Despite extensive research and different tools have been developed it still remains a challenge for early detection of this disease. FTIR imaging has been used to diagnose and differentiate the molecular differences between normal and diseased tissues. Methods: Fourier Transform Infrared Spectroscopy (FTIR) is able to measure biochemical changes in tissue, cell and biofluids based on the vibrational signature of their components. This technique enables to the distribution and structure of lipids, proteins, nucleic acids as well as other metabolites. These differences depended on the type and the grade of cancer. Results: We emphasize here, that the FTIR spectroscopy and imaging can be considered as a promising technique and will find its place on the detection of this dreadful disease because of high sensitivity, accuracy and inexpensive technique. Now the medical community started using and accepting this technique for early stage cancer detection. We discussed this technique and the several challenges in its application for the diagnosis of cancer in regards of sample preparations, data interpretation, and data analysis. The sensitivity of chemotherapy drugs on individual specific has also discussed. Conclusion: So far progressed has done with the FTIR imaging in understanding of cancer disease pathology. However, more research is needed in this field and it is necessary to understand the morphology and biology of the sample before using the spectroscopy and imaging because invaluable information to be figured out.

Place, publisher, year, edition, pages
BENTHAM SCIENCE PUBL LTD, 2018
Keywords
Infrared imaging, cancers, diagnosis, therapeutic drugs, FTIR
National Category
Cancer and Oncology Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-357068 (URN)10.2174/0929867324666170523121314 (DOI)000429247300005 ()
Funder
VINNOVA
Available from: 2018-08-13 Created: 2018-08-13 Last updated: 2018-08-15Bibliographically approved
Qin, T., Zhang, P., Wani, I. H., Han, Y., LEIFER, K., Nikolajeff, F. & Engqvist, H. (2017). A general strategy for template-free and low-cost synthesis of inorganic hollow spheres. Powder Technology, 319, 163-171
Open this publication in new window or tab >>A general strategy for template-free and low-cost synthesis of inorganic hollow spheres
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2017 (English)In: Powder Technology, ISSN 0032-5910, E-ISSN 1873-328X, Vol. 319, p. 163-171Article in journal (Refereed) Published
Abstract [en]

Inorganic hollow spheres have a great potential in many fields, such as calcium phosphate (Ca3(PO4)2) as carriers of active ingredients and local delivery. They are typically synthesized by the methods that reply on template-based strategies. However, the template residue and energy consumption during template removal are drawbacks. Currently developed template-free methods remain challenges such as time, cost and complicated procedures. In this paper, we introduce a general low-cost and template-free precipitation method with simple procedure. A series of inorganic hollow spheres, including calcium phosphate, calcium fluoride, strontium phosphate, strontium fluoride, barium phosphate and barium fluoride via magnesium were successfully synthesized, respectively. Based on these experimental results, a new model is proposed to explain the mechanism of the hollow inorganic spheres formation. This paper provides a general method to synthesize inorganic hollow spheres, which may have an important indication to other systems.

Keywords
Inorganic, Spheres, Hollow, Mechanism
National Category
Materials Engineering
Identifiers
urn:nbn:se:uu:diva-333956 (URN)10.1016/j.powtec.2017.06.051 (DOI)000407982600016 ()
Available from: 2017-12-13 Created: 2017-12-13 Last updated: 2019-04-24Bibliographically approved
Kumar, S., Liu, X., Borondics, F., Xiao, Q., Feng, R., Goormaghtigh, E. & Nikolajeff, F. (2017). Insights into Biochemical Alteration in Cancer-Associated Fibroblasts by using Novel Correlative Spectroscopy. ChemistryOpen, 6(1), 149-157
Open this publication in new window or tab >>Insights into Biochemical Alteration in Cancer-Associated Fibroblasts by using Novel Correlative Spectroscopy
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2017 (English)In: ChemistryOpen, ISSN 2191-1363, Vol. 6, no 1, p. 149-157Article in journal (Refereed) Published
Abstract [en]

The microenvironment of a tumor changes chemically and morphologically during cancer progression. Cancer-stimulated fibroblasts promote tumor growth, however, the mechanism of the transition to a cancer-stimulated fibroblast remains elusive. Here, the multi-modal spectroscopic methods Fourier transform infrared imaging (FTIRI), X-ray absorption spectroscopy (XAS) and X-ray fluorescence imaging (XFI) are used to characterize molecular and atomic alterations that occur in cancerstimulated fibroblasts. In addition to chemical changes in lipids (olefinic and acyl chain) and protein aggregation observed with FTIRI, a new infrared biomarker for oxidative stress in stimulated fibroblasts is reported. Oxidative stress is observed to cause lipid peroxidation, which leads to the appearance of a new band at 1721 cm(-1), assigned to 4-hydroxynonenal. Complementary to FTIRI, XFI is well suited to determining atom concentrations and XAS can reveal the speciation of individual elements. XFI reveals increased concentrations of P, S, K, Ca within stimulated fibroblasts. Furthermore, XAS studies reveal alterations in the speciation of S and Ca in stimulated fibroblasts, which might provide insight into the mechanisms of cancer progression. Using XFI, not only is the concentration change of individual elements observed, but also the subcellular localization. This study demonstrates the wealth of biochemical information provided by a multi-modal imaging approach and highlights new avenues for future research into the microenvironment of breast tumors.

Place, publisher, year, edition, pages
WILEY-V C H VERLAG GMBH, 2017
Keywords
cancer, fibroblasts, Fourier transform infrared, imaging, X-ray absorption spectroscopy, X-ray fluorescence
National Category
Chemical Sciences Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-320702 (URN)10.1002/open.201600102 (DOI)000397240200020 ()28168160 (PubMedID)
Available from: 2017-04-24 Created: 2017-04-24 Last updated: 2017-11-29Bibliographically approved
Qin, T., Han, Y., Zhang, P., Wani, I. H., Nikolajeff, F., Leifer, K. & Engqvist, H. (2017). Template-free synthesis of phosphate-based spheres via modified supersaturated phosphate buffer solutions. Journal of materials science. Materials in medicine, 28(7), Article ID 99.
Open this publication in new window or tab >>Template-free synthesis of phosphate-based spheres via modified supersaturated phosphate buffer solutions
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2017 (English)In: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 28, no 7, article id 99Article in journal (Refereed) Published
Abstract [en]

Modified supersaturated phosphate buffer solutions were used to synthesize phosphate-based spheres, including calcium phosphate (CaP), strontium phosphate (SrP) and barium phosphate (BaP). A series of ions concentrations in the modified phosphate buffer solutions were investigated in order to study their effects in precipitates morphologies. During synthesis, it was found that magnesium was the key factor in sphere formation. The morphologies of calcium phosphate, strontium phosphate and barium phosphate precipitates varied as the concentration of magnesium ions varied. When sufficient magnesium was provided, the precipitates appeared spherical, and the diameter was in range of 0.5-2 mu m. The morphologies, compositions and structure of spheres were characterized by x-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and N-2 adsorption analysis. Moreover, the application of magnesium substituted calcium phosphate spheres in dentin tubules occlusion was investigated.

Place, publisher, year, edition, pages
SPRINGER, 2017
National Category
Medical Materials
Identifiers
urn:nbn:se:uu:diva-329001 (URN)10.1007/s10856-017-5911-x (DOI)000403777800001 ()28534282 (PubMedID)
Available from: 2017-09-06 Created: 2017-09-06 Last updated: 2019-04-24Bibliographically approved
Lopez-Lorente, A., Wang, P., Sieger, M., Vargas Catalan, E., Karlsson, M., Nikolajeff, F., . . . Mizaikoff, B. (2016). Mid-infrared thin-film diamond waveguides combined with tunable quantum cascade lasers for analyzing the secondary structure of proteins. Physica Status Solidi (a) applications and materials science, 213(8), 2117-2123
Open this publication in new window or tab >>Mid-infrared thin-film diamond waveguides combined with tunable quantum cascade lasers for analyzing the secondary structure of proteins
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2016 (English)In: Physica Status Solidi (a) applications and materials science, ISSN 1862-6300, E-ISSN 1862-6319, Vol. 213, no 8, p. 2117-2123Article in journal (Refereed) Published
Abstract [en]

Diamond has excellent optical properties including broadband transmissivity, low self-absorption, and a high refractive index, which have prompted its use for optical sensing applications. Thin-film diamond strip waveguides (DSWGs) combined with tunable quantum cascade lasers (tQCLs) providing an emission wavelength range of 5.78-6.35 mu m (1735-1570 cm(-1)) have been used to obtain mid-infrared (MIR) spectra of proteins, thereby enabling the analysis of their secondary structure via the amide I band. Three different proteins were analyzed, namely bovine serum albumin (BSA), myoglobin, and gamma-globulin. The secondary structure of BSA and myoglobin has a major contribution of a-helices, whereas gamma-globulins are rich in beta-sheet structures, which is reflected in the amide I band. Acomparison of the spectra obtained via the combination of the tQCL and DSWG with spectra obtained using conventional Fourier transform infrared (FTIR) spectroscopy and a commercial diamond attenuated total reflection (ATR) element has been performed. It is shown that the main features evident in FTIR-ATR spectra are also obtained using tQCL-DSWG sensors.

National Category
Other Physics Topics Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-282255 (URN)10.1002/pssa.201600134 (DOI)000385223900016 ()
Available from: 2016-04-04 Created: 2016-04-04 Last updated: 2017-11-30Bibliographically approved
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