uu.seUppsala University Publications
Change search
Link to record
Permanent link

Direct link
BETA
Hajdu, J
Alternative names
Publications (10 of 62) Show all publications
Bielecki, J., Hantke, M. F., Daurer, B. J., Reddy, H. K. N., Hasse, D., Larsson, D. S. D., . . . Maia, F. R. N. (2019). Electrospray sample injection for single-particle imaging with x-ray lasers. Science Advances, 5(5), Article ID eaav8801.
Open this publication in new window or tab >>Electrospray sample injection for single-particle imaging with x-ray lasers
Show others...
2019 (English)In: Science Advances, E-ISSN 2375-2548, Vol. 5, no 5, article id eaav8801Article in journal (Refereed) Published
National Category
Biophysics
Identifiers
urn:nbn:se:uu:diva-387970 (URN)10.1126/sciadv.aav8801 (DOI)000470125000080 ()31058226 (PubMedID)
Available from: 2019-05-03 Created: 2019-06-27 Last updated: 2019-06-27Bibliographically approved
Helliwell, J. R., Minor, W., Weiss, M. S., Garman, E. F., Read, R. J., Newman, J., . . . Baker, E. N. (2019). Findable Accessible Interoperable Re-usable (FAIR) diffraction data are coming to protein crystallography. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 75, 455-457
Open this publication in new window or tab >>Findable Accessible Interoperable Re-usable (FAIR) diffraction data are coming to protein crystallography
Show others...
2019 (English)In: ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, ISSN 2059-7983, Vol. 75, p. 455-457Article in journal, Editorial material (Other academic) Published
Place, publisher, year, edition, pages
INT UNION CRYSTALLOGRAPHY, 2019
Keywords
FAIR, diffraction data, IUCr policy
National Category
Structural Biology
Identifiers
urn:nbn:se:uu:diva-384082 (URN)10.1107/S2059798319004844 (DOI)000467046700001 ()31063147 (PubMedID)
Note

This editorial article is simultaneously published by the IUCr in the journal IUCrJ (doi:10.1107/S2052252519005918) and in the journal Journal of applied crystallography (doi:10.1107/S1600576719005922) and in the journal Acta Crystallographica Section F structural biology communications (doi: 10.1107/S2053230X19005909)

Available from: 2019-06-18 Created: 2019-06-18 Last updated: 2019-06-18Bibliographically approved
Mühlig, K., Ganan-Calvo, A. M., Andreasson, J., Larsson, D. S., Hajdu, J. & Svenda, M. (2019). Nanometre-sized droplets from a gas dynamic virtual nozzle. Journal of applied crystallography, 52, 800-808
Open this publication in new window or tab >>Nanometre-sized droplets from a gas dynamic virtual nozzle
Show others...
2019 (English)In: Journal of applied crystallography, ISSN 0021-8898, E-ISSN 1600-5767, Vol. 52, p. 800-808Article in journal (Refereed) Published
Abstract [en]

This paper reports on improved techniques to create and characterize nanometre-sized droplets from dilute aqueous solutions by using a gas dynamic virtual nozzle (GDVN). It describes a method to measure the size distribution of uncharged droplets, using an environmental scanning electron microscope, and provides theoretical models for the droplet sizes created. The results show that droplet sizes can be tuned by adjusting the gas and liquid flow rates in the GDVN, and at the lowest liquid flow rates, the size of the water droplets peaks at about 120nm. This droplet size is similar to droplet sizes produced by electrospray ionization but requires neither electrolytes nor charging of the solution. The results presented here identify a new operational regime for GDVNs and show that predictable droplet sizes, comparable to those obtained by electrospray ionization, can be produced by purely mechanical means in GDVNs.

Place, publisher, year, edition, pages
INT UNION CRYSTALLOGRAPHY, 2019
Keywords
aerosols, droplet size, gas dynamic virtual nozzles (GDVNs), environmental scanning electron microscopy, coherent X-ray diffractive imaging (CXDI), single particles, sample delivery, structural biology, nanoscience
National Category
Physical Chemistry
Identifiers
urn:nbn:se:uu:diva-391281 (URN)10.1107/S1600576719008318 (DOI)000477717400013 ()
Funder
Swedish Research CouncilKnut and Alice Wallenberg FoundationEU, European Research Council
Available from: 2019-08-22 Created: 2019-08-22 Last updated: 2019-08-22Bibliographically approved
Klimesova, E., Kulyk, O., Gu, Y., Dittrich, L., Korn, G., Hajdu, J., . . . Andreasson, J. (2019). Plasma channel formation in NIR laser-irradiated carrier gas from an aerosol nanoparticle injector. Scientific Reports, 9, Article ID 8851.
Open this publication in new window or tab >>Plasma channel formation in NIR laser-irradiated carrier gas from an aerosol nanoparticle injector
Show others...
2019 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, article id 8851Article in journal (Refereed) Published
Abstract [en]

Aerosol nanoparticle injectors are fundamentally important for experiments where container-free sample handling is needed to study isolated nanoparticles. The injector consists of a nebuliser, a differential pumping unit, and an aerodynamic lens to create and deliver a focused particle beam to the interaction point inside a vacuum chamber. The tightest focus of the particle beam is close to the injector tip. The density of the focusing carrier gas is high at this point. We show here how this gas interacts with a near infrared laser pulse (800 nm wavelength, 120 fs pulse duration) at intensities approaching 10(16) Wcm(-2). We observe acceleration of gas ions to kinetic energies of 100s eV and study their energies as a function of the carrier gas density. Our results indicate that field ionisation by the intense near-infrared laser pulse opens up a plasma channel behind the laser pulse. The observations can be understood in terms of a Coulomb explosion of the created underdense plasma channel. The results can be used to estimate gas background in experiments with the injector and they open up opportunities for a new class of studies on electron and ion dynamics in nanoparticles surrounded by a low-density gas.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP, 2019
National Category
Atom and Molecular Physics and Optics
Identifiers
urn:nbn:se:uu:diva-390094 (URN)10.1038/s41598-019-45120-3 (DOI)000472136900025 ()31221980 (PubMedID)
Funder
Swedish Research CouncilKnut and Alice Wallenberg Foundation
Available from: 2019-08-05 Created: 2019-08-05 Last updated: 2019-08-05Bibliographically approved
Pietrini, A., Bielecki, J., Timneanu, N., Hantke, M. F., Andreasson, J., Loh, N. D., . . . Nettelblad, C. (2018). A statistical approach to detect protein complexes at X-ray free electron laser facilities. Communications Physics, 1, 92:1-11, Article ID 92.
Open this publication in new window or tab >>A statistical approach to detect protein complexes at X-ray free electron laser facilities
Show others...
2018 (English)In: Communications Physics, E-ISSN 2399-3650, Vol. 1, p. 92:1-11, article id 92Article in journal (Refereed) Published
National Category
Biophysics
Identifiers
urn:nbn:se:uu:diva-369876 (URN)10.1038/s42005-018-0092-6 (DOI)000452676300003 ()
Projects
eSSENCE
Available from: 2018-12-07 Created: 2018-12-17 Last updated: 2019-05-06Bibliographically approved
Lundholm, I. V., Sellberg, J. A., Ekeberg, T., Hantke, M. F., Okamoto, K., van der Schot, G., . . . Maia, F. R. N. (2018). Considerations for three-dimensional image reconstruction from experimental data in coherent diffractive imaging. IUCrJ, 5, 531-541
Open this publication in new window or tab >>Considerations for three-dimensional image reconstruction from experimental data in coherent diffractive imaging
Show others...
2018 (English)In: IUCrJ, ISSN 0972-6918, E-ISSN 2052-2525, Vol. 5, p. 531-541Article in journal (Refereed) Published
National Category
Biophysics
Identifiers
urn:nbn:se:uu:diva-360034 (URN)10.1107/S2052252518010047 (DOI)000444010100003 ()
Projects
eSSENCE
Available from: 2018-09-01 Created: 2018-09-09 Last updated: 2019-07-01Bibliographically approved
Okamoto, K., Miyazaki, N., Reddy, H. K. .., Hantke, M. F., Maia, F. R. .., Larsson, D. S., . . . Svenda, M. (2018). Cryo-EM structure of a Marseilleviridae virus particle reveals a large internal microassembly. Virology, 516, 239-245, Article ID S0042-6822(18)30028-X.
Open this publication in new window or tab >>Cryo-EM structure of a Marseilleviridae virus particle reveals a large internal microassembly
Show others...
2018 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 516, p. 239-245, article id S0042-6822(18)30028-XArticle in journal (Refereed) Published
Abstract [en]

Nucleocytoplasmic large DNA viruses (NCLDVs) blur the line between viruses and cells. Melbournevirus (MelV, family Marseilleviridae) belongs to a new family of NCLDVs. Here we present an electron cryo-microscopy structure of the MelV particle, with the large triangulation number T = 309 constructed by 3080 pseudo-hexagonal capsomers. The most distinct feature of the particle is a large and dense body (LDB) consistently found inside all particles. Electron cryo-tomography of 147 particles shows that the LDB is preferentially located in proximity to the probable lipid bilayer. The LDB is 30 nm in size and its density matches that of a genome/protein complex. The observed LDB reinforces the structural complexity of MelV, setting it apart from other NCLDVs.

Keywords
Amoeba, Capsid, Cryo-electron microscopy, Marseilleviridae, Melbournevirus, NCLDV, Protein complex, Structure, Tomography, Virus
National Category
Biophysics
Identifiers
urn:nbn:se:uu:diva-370071 (URN)10.1016/j.virol.2018.01.021 (DOI)000428004800025 ()29407382 (PubMedID)
Funder
Swedish Research Council, 628-20081109 822-2010-6157 822-2012-5260 828-2012-108Knut and Alice Wallenberg Foundation, KAW-2011.081EU, European Research Council, ERC-291602The Swedish Foundation for International Cooperation in Research and Higher Education (STINT), JA2014-5721
Available from: 2018-12-18 Created: 2018-12-18 Last updated: 2019-08-25Bibliographically approved
Gorkhover, T., Ulmer, A., Ferguson, K., Bucher, M., Maia, F. R. N., Bielecki, J., . . . Bostedt, C. (2018). Femtosecond X-ray Fourier holography imaging of free-flying nanoparticles [Letter to the editor]. Nature Photonics, 12, 150-153
Open this publication in new window or tab >>Femtosecond X-ray Fourier holography imaging of free-flying nanoparticles
Show others...
2018 (English)In: Nature Photonics, ISSN 1749-4885, E-ISSN 1749-4893, Vol. 12, p. 150-153Article in journal, Letter (Refereed) Published
National Category
Biophysics
Identifiers
urn:nbn:se:uu:diva-345590 (URN)10.1038/s41566-018-0110-y (DOI)000426153800014 ()
Projects
eSSENCE
Available from: 2018-02-26 Created: 2018-03-09 Last updated: 2019-07-01Bibliographically approved
Allen, A. J., Hajdu, J. & McIntyre, G. J. (2018). Journal of Applied Crystallography: the first 50 years and beyond. Journal of applied crystallography, 51(Part: 2), 233-234
Open this publication in new window or tab >>Journal of Applied Crystallography: the first 50 years and beyond
2018 (English)In: Journal of applied crystallography, ISSN 0021-8898, E-ISSN 1600-5767, Vol. 51, no Part: 2, p. 233-234Article in journal, Editorial material (Other academic) Published
Abstract [en]

The Editors of Journal of Applied Crystallography mark the journal's 50th anniversary.

Keywords
editorial, Journal of Applied Crystallography
National Category
Condensed Matter Physics
Identifiers
urn:nbn:se:uu:diva-357032 (URN)10.1107/S1600576718004478 (DOI)000429090100001 ()
Available from: 2018-08-10 Created: 2018-08-10 Last updated: 2018-08-10Bibliographically approved
Wiedorn, M. O., Oberthuer, D., Bean, R., Schubert, R., Werner, N., Abbey, B., . . . Barty, A. (2018). Megahertz serial crystallography. Nature Communications, 9, Article ID 4025.
Open this publication in new window or tab >>Megahertz serial crystallography
Show others...
2018 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 9, article id 4025Article in journal (Refereed) Published
Abstract [en]

The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a beta-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.

Place, publisher, year, edition, pages
Nature Publishing Group, 2018
National Category
Physical Chemistry
Identifiers
urn:nbn:se:uu:diva-367393 (URN)10.1038/s41467-018-06156-7 (DOI)000446016400003 ()30279492 (PubMedID)
Funder
EU, European Research Council, 609920Wellcome trustGerman Research Foundation (DFG)
Available from: 2018-12-04 Created: 2018-12-04 Last updated: 2018-12-04Bibliographically approved
Organisations

Search in DiVA

Show all publications