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Roman, Erika
Publications (10 of 72) Show all publications
Lundberg, S., Högman, C. & Roman, E. (2019). Adolescent Exploratory Strategies and Behavioral Types in the Multivariate Concentric Square Field (TM) Test. Frontiers in Behavioral Neuroscience, 13, Article ID 41.
Open this publication in new window or tab >>Adolescent Exploratory Strategies and Behavioral Types in the Multivariate Concentric Square Field (TM) Test
2019 (English)In: Frontiers in Behavioral Neuroscience, ISSN 1662-5153, E-ISSN 1662-5153, Vol. 13, article id 41Article in journal (Refereed) Published
Abstract [en]

Adolescence is an important developmental phase with extensive changes in behavior due to remodeling of the brain and hormonal systems. Validation of animal behavioral tests in this age group is therefore of importance as differences to adult behavior are often not clarified. The aim of the present study was to investigate adolescent behavior in the multivariate concentric square field (TM) (MCSF) test and its relationship to other common behavioral tests as well as to a literature dataset of adult animals. Sixty adolescent male Wistar rats were tested in the MCSF and one of four reference tests; the elevated plus maze, the open field with or without start box, or the social play behavior test. Additionally, 12 animals were tested twice in the MCSF. When analyzing the first encounter with the MCSF test, a distinct grouping of the individuals into three behavioral types was observed. Approximately 20% of the animals had high levels of activity and an additional 20% had high levels of shelter seeking-behavior, these groups composed the outlying behavioral types named Explorers and Shelter seekers, respectively, which were distinct from the Main type of animals. When tested in the MCSF for a second time, the adolescent animals showed a recollection of the arena as they changed their behavior in relation to the first encounter. When comparing the MCSF performance to the reference tests, a relationship was found between the MCSF and the other behavioral test entailing forced exploration, while no relationship was found between the MCSF and social play. The adolescent behavioral profile was characterized by decreased risk assessment and a different activity profile than adults. In conclusion, the MCSF test is useful for profiling adolescent rats but the behavioral interpretation differs from that of adults due to differences in behavioral manifestation during adolescence and the presence of natural subgroups. Adolescent exploration shows a relationship across tests, but the MCSF gives more information than any of the other behavioral tests based on forced exploration. Further studies into the neurobiology behind the behavioral types and how different manipulations affect the distribution into the behavioral types are of interest.

Place, publisher, year, edition, pages
FRONTIERS MEDIA SA, 2019
Keywords
behavior, elevated plus maze, open field, phenotyping, risk taking, social play, validation, Wistar rat
National Category
Neurosciences
Identifiers
urn:nbn:se:uu:diva-379899 (URN)10.3389/fnbeh.2019.00041 (DOI)000460350200001 ()30886574 (PubMedID)
Available from: 2019-03-22 Created: 2019-03-22 Last updated: 2019-03-22Bibliographically approved
Thörnqvist, P.-O., McCarrick, S., Ericsson, M., Roman, E. & Winberg, S. (2019). Bold zebrafish (Danio rerio) express higher levels of delta opioid and dopamine D2 receptors in the brain compared to shy fish. Behavioural Brain Research, 359, 927-934
Open this publication in new window or tab >>Bold zebrafish (Danio rerio) express higher levels of delta opioid and dopamine D2 receptors in the brain compared to shy fish
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2019 (English)In: Behavioural Brain Research, ISSN 0166-4328, E-ISSN 1872-7549, Vol. 359, p. 927-934Article in journal (Refereed) Published
Abstract [en]

Individual variation in coping with environmental challenges is a well-known phenomenon across vertebrates, including teleost fish. Dopamine is the major transmitter in the brain reward networks, and important for motivational processes and stress coping. Functions of the endogenous opioid system are not well studied in teleosts. However, in mammals the activity in the brain reward networks is regulated by the endogenous opioid system. This study aimed at investigating if there was a correlation between risk-taking behavior and the expression of dopamine and opioid receptors in the zebrafish (Danio rerio) brain. Risk-taking behavior was assessed in a novel tank diving test, and the most extreme high risk taking, i.e. bold, and low risk taking, i.e. shy, fish were sampled for qPCR analysis of whole brain gene expression. The expression analysis showed a significantly higher expression of the dopamine D2 receptors (drd2a and drd2b) and the delta opioid receptor (DOR; oprd1b) in bold compared to shy fish. Besides reward and reinforcing properties, DORs are also involved in emotional responses. Dopamine D2 receptors are believed to be important for active stress coping in rodents, and taken together the results of the current study suggest similar functions in zebrafish. However, additional experiments are required to clarify how dopamine and opioid receptor activation affect behavior and stress coping in this species.

Place, publisher, year, edition, pages
Elsevier, 2019
Keywords
Animal personality, Behavior, Boldness, Dopamine receptor, Opioid receptor, Risk taking, Shyness
National Category
Neurosciences
Identifiers
urn:nbn:se:uu:diva-376722 (URN)10.1016/j.bbr.2018.06.017 (DOI)000456222600107 ()29935279 (PubMedID)
Funder
Swedish Research Council
Available from: 2019-02-11 Created: 2019-02-11 Last updated: 2019-02-11Bibliographically approved
Mustafa, A., Thörnqvist, P.-O., Roman, E. & Winberg, S. (2019). The aggressive spiegeldanio, carrying a mutation in the fgfr1a gene, has no advantage in dyadic fights with zebrafish of the AB strain. Behavioural Brain Research, 370, Article ID 111942.
Open this publication in new window or tab >>The aggressive spiegeldanio, carrying a mutation in the fgfr1a gene, has no advantage in dyadic fights with zebrafish of the AB strain
2019 (English)In: Behavioural Brain Research, ISSN 0166-4328, E-ISSN 1872-7549, Vol. 370, article id 111942Article in journal (Refereed) Published
Abstract [en]

Zebrafish which carries a mutation in the fibroblast growth factor receptor 1A (fgfr1a), also known as spiegeldanio (spd), has previously been reported to be bolder and more aggressive than wildtype (AB) zebrafish. However, in previous studies aggression has been quantified in mirror tests. In dyadic fights the behavior of the combatants is modified by the behavior of their opponent, and fighting a mirror has been reported to have different effects on brain gene expression and brain monoaminergic systems. In the present study aggression was quantified in fgfr1a mutants and AB zebrafish using a mirror test after which the fish were allowed to interact in pairs, either consisting of two fgfr1a mutants or one AB and one fgfr1a mutant fish. Following dyadic interaction aggressive behavior was again quantified in individual fish in a second mirror test after which the fish were sacrificed and brain tissue analyzed for monoamines and monoamine metabolites. The results confirm that fgfr1a mutants are more aggressive than AB zebrafish in mirror tests. However, fgfr1a mutant fish did not have any advantage in fights for social dominance, and agonistic behavior of fgfr1a mutants did not differ from that of AB fish during dyadic interactions. Moreover, as the AB fish, fgfr1a mutant fish losing dyadic interactions showed a typical loser effect and social subordination resulted in an activation of the brain serotonergic system in fgfr1a mutants as well as in AB fish. Overall the effects of dyadic interaction were similar in fgfr1a mutant fish and zebrafish of the AB strain.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV, 2019
Keywords
Aggression, Agonistic behavior, Dominance, Dopamine, Scrotonin
National Category
Developmental Biology Medical Genetics
Identifiers
urn:nbn:se:uu:diva-390372 (URN)10.1016/j.bbr.2019.111942 (DOI)000474323900003 ()31085203 (PubMedID)
Funder
Swedish Research Council
Available from: 2019-08-12 Created: 2019-08-12 Last updated: 2019-08-12Bibliographically approved
Roman, E., Brunberg, R., Mustafa, A., Thörnqvist, P.-O. & Winberg, S. (2018). Behavioral profiling using a modified version of the zebrafish multivariate concentric square field™ (zMCSF) test. In: Grant R, Allen T, Spink A, Sullivan M (Ed.), Measuring Behavior 2018: 11th International Conference on Methods and Techniques in Behavioral Research. Paper presented at Measuring Behavior, Manchester, 5-8 June, 2018. (pp. 27-29).
Open this publication in new window or tab >>Behavioral profiling using a modified version of the zebrafish multivariate concentric square field™ (zMCSF) test
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2018 (English)In: Measuring Behavior 2018: 11th International Conference on Methods and Techniques in Behavioral Research / [ed] Grant R, Allen T, Spink A, Sullivan M, 2018, p. 27-29Conference paper, Published paper (Refereed)
Keywords
behavioral profiling, zMCSF, multivariate concentric square field, zebrafish
National Category
Neurosciences
Identifiers
urn:nbn:se:uu:diva-356583 (URN)978-1-910029-39-8 (ISBN)
Conference
Measuring Behavior, Manchester, 5-8 June, 2018.
Available from: 2018-08-01 Created: 2018-08-01 Last updated: 2018-08-01Bibliographically approved
Tjernström, N. & Roman, E. (2018). Characterization of behavior and voluntary alcohol intake in Wistar and Lister Hooded rats. In: Grant R, Allen T, Spink A, Sullivan M (Ed.), Measuring Behavior 2018: 11th International Conference on Methods and Techniques in Behavioral Research. Paper presented at Measuring Behavior, Manchester, 5-8 June, 2018 (pp. 437-442).
Open this publication in new window or tab >>Characterization of behavior and voluntary alcohol intake in Wistar and Lister Hooded rats
2018 (English)In: Measuring Behavior 2018: 11th International Conference on Methods and Techniques in Behavioral Research / [ed] Grant R, Allen T, Spink A, Sullivan M, 2018, p. 437-442Conference paper, Published paper (Refereed)
Keywords
behavior, multivariate concentric square field, behavioral profiling, strain differences, MCSF, ethanol
National Category
Neurosciences
Identifiers
urn:nbn:se:uu:diva-356582 (URN)978-1-910029-39-8 (ISBN)
Conference
Measuring Behavior, Manchester, 5-8 June, 2018
Available from: 2018-08-01 Created: 2018-08-01 Last updated: 2018-08-01Bibliographically approved
Lundberg, S., Martinsson, M., Nylander, I. & Roman, E. (2017). Altered corticosterone levels and social play behavior after prolonged maternal separation in adolescent male but not female Wistar rats. Hormones and Behavior, 87, 137-144
Open this publication in new window or tab >>Altered corticosterone levels and social play behavior after prolonged maternal separation in adolescent male but not female Wistar rats
2017 (English)In: Hormones and Behavior, ISSN 0018-506X, E-ISSN 1095-6867, Vol. 87, p. 137-144Article in journal (Refereed) Published
Abstract [en]

Early-life socio-environmental factors are crucial for normal developmental processes; adverse experiences early in life can therefore lead to detrimental effects in several physiological systems. The aim of this study was to examine short-term effects of early adverse experiences in a maternal separation (MS) rodent model. In this study two separation conditions were used: daily 15-(MS15) or 360-min (MS360) separation of the litter from the dam during postnatal day 1-21. In early adolescence, male and female offspring were subjected to a single-isolation procedure with analysis of corticosterone levels prior to and after isolation. In addition, social play behavior was assessed during mid-adolescence. There was a clear difference between male and female offspring in both tests performed. There was no difference in corticosterone levels between the female MS groups, whereas MS360 males showed higher baseline and recovery corticosterone levels than MS15 males. The amount of pinning, a specific social play behavior, was affected by rearing with MS360 males having a higher frequency than MS15 males, while there was no difference between the female MS groups. The observation that males but not females are affected by MS360 has previously been reported for adult animals, and herein we show that this difference is present already in adolescence. Changes in corticosterone levels and social behavior following early-life adversity have been associated with adult behavioral alterations, and our results confirm that these changes emerge already within adolescence.

National Category
Endocrinology and Diabetes
Identifiers
urn:nbn:se:uu:diva-316957 (URN)10.1016/j.yhbeh.2016.11.016 (DOI)000392905500016 ()27884596 (PubMedID)
Funder
Swedish Research Council, K2012-61 x-22090-01-3
Available from: 2017-03-08 Created: 2017-03-08 Last updated: 2017-11-29Bibliographically approved
Lundberg, S., Abelson, K., Nylander, I. & Roman, E. (2017). Few long-term consequences after prolonged maternal separation in female Wistar rats. PLoS ONE, 12(12), Article ID e0190042.
Open this publication in new window or tab >>Few long-term consequences after prolonged maternal separation in female Wistar rats
2017 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 12, article id e0190042Article in journal (Refereed) Published
Abstract [en]

Environmental factors during the early-life period are known to have long-term consequences for the adult phenotype. An intimate interplay between genes and environment shape the individual and may affect vulnerability for psychopathology in a sex-dependent manner. A rodent maternal separation model was here used to study the long-term effects of different early-life rearing conditions on adult behavior, HPA axis activity and long-term voluntary alcohol intake. Litters were subjected to 15 (MS15) or 360 min (MS360) of daily maternal separation during postnatal day 1–21. In adulthood, the behavioral profiles were investigated using the multivariate concentric square field™ (MCSF) test or examined for HPA axis reactivity by cat-odor exposure with subsequent characterization of voluntary alcohol intake and associated changes in HPA axis activity. Adult female offspring showed mostly no, or only minor, effects of MS360 on behavior, HPA axis reactivity and long-term alcohol intake. Instead, more pronounced effects were found dependent on changes in the female’s natural hormonal cycle or by the choice of animal supplier. However, changes were revealed in corticosterone load after long-term alcohol access, as females subjected to MS360 had higher concentrations of fecal corticosterone. The present findings are in line with and expand on previous studies on the long-term effects of maternal separation and the sex-dependent effects, with regard to behavior and voluntary alcohol intake. Why female rats show increased resilience compared to males using the present experimental protocol for maternal separation remains to be further investigated.

Place, publisher, year, edition, pages
PUBLIC LIBRARY SCIENCE, 2017
Keywords
Alcohol intake, behavior, estrus cycle, gonadal hormone, handling, HPA axis reactivity, maternal deprivation, multivariate concentric square field test, MCSF, resilience, sex differences, stress reactivity, supplier differences
National Category
Neurosciences
Identifiers
urn:nbn:se:uu:diva-322548 (URN)10.1371/journal.pone.0190042 (DOI)000418587400082 ()29267376 (PubMedID)
Funder
Swedish Research Council, K2012-61-22090-01-3, K2005-04X-12588-08AThe Swedish Brain FoundationAFA Insurance
Available from: 2017-05-24 Created: 2017-05-24 Last updated: 2018-02-15Bibliographically approved
Wood, C. M., Nicolas, C. S., Choi, S.-L., Roman, E., Nylander, I., Fernandez-Teruel, A., . . . Lodge, D. (2017). Prevalence and influence of cys407* Grm2 mutation in Hannover-derived Wistar rats: mGlu2 receptor loss links to alcohol intake, risk taking and emotional behaviour.. Neuropharmacology, 115, 128-138
Open this publication in new window or tab >>Prevalence and influence of cys407* Grm2 mutation in Hannover-derived Wistar rats: mGlu2 receptor loss links to alcohol intake, risk taking and emotional behaviour.
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2017 (English)In: Neuropharmacology, ISSN 0028-3908, E-ISSN 1873-7064, Vol. 115, p. 128-138Article, review/survey (Refereed) Published
Abstract [en]

Modulation of metabotropic glutamate 2 (mGlu2) receptor function has huge potential for treating psychiatric and neurological diseases. Development of drugs acting on mGlu2 receptors depends on the development and use of translatable animal models of disease. We report here a stop codon mutation at cysteine 407 in Grm2 (cys407*) that is common in some Wistar rats. Therefore, researchers in this field need to be aware of strains with this mutation. Our genotypic survey found widespread prevalence of the mutation in commercial Wistar strains, particularly those known as Han Wistar. Such Han Wistar rats are ideal for research into the separate roles of mGlu2 and mGlu3 receptors in CNS function. Previous investigations, unknowingly using such mGlu2 receptor-lacking rats, provide insights into the role of mGlu2 receptors in behaviour. The Grm2 mutant rats, which dominate some selectively bred lines, display characteristics of altered emotionality, impulsivity and risk-related behaviours and increased voluntary alcohol intake compared with their mGlu2 receptor-competent counterparts. In addition, the data further emphasize the potential therapeutic role of mGlu2 receptors in psychiatric and neurological disease, and indicate novel methods of studying the role of mGlu2 and mGlu3 receptors.

Keywords
Metabotropic glutamate receptor; mGlu2; Grm2 mutation; Wistar rats; Han Wistar rats; Emotionality; Anxiety; Selectively bred rats; Alcohol preference
National Category
Neurosciences Pharmaceutical Sciences
Identifiers
urn:nbn:se:uu:diva-318629 (URN)10.1016/j.neuropharm.2016.03.020 (DOI)000400218600013 ()26987983 (PubMedID)
Funder
Eli Lilly, PS1201341872-P (MINECO); 2014SGR-1587 (DGR)
Available from: 2017-03-27 Created: 2017-03-27 Last updated: 2018-01-13
Roman, E., Tjernström, N. & Winberg, S. (2016). Description of a multivariate behavioral test arena for zebrafish – the zebrafish multivariate concentric square field test. In: Spink A, Riedel G, Zhou l, Teekens L, Albatal R, Gurrin C (Ed.), Proceedings of Measuring Behavior 2016: . Paper presented at Measuring Behavior 2016, Dublin, May 25-27 2016. (pp. 4-6). Dublin, Ireland: Measuring Behavior
Open this publication in new window or tab >>Description of a multivariate behavioral test arena for zebrafish – the zebrafish multivariate concentric square field test
2016 (English)In: Proceedings of Measuring Behavior 2016 / [ed] Spink A, Riedel G, Zhou l, Teekens L, Albatal R, Gurrin C, Dublin, Ireland: Measuring Behavior , 2016, p. 4-6Conference paper, Published paper (Refereed)
Place, publisher, year, edition, pages
Dublin, Ireland: Measuring Behavior, 2016
National Category
Neurosciences
Identifiers
urn:nbn:se:uu:diva-318632 (URN)978-1-873769-59-1 (ISBN)
Conference
Measuring Behavior 2016, Dublin, May 25-27 2016.
Available from: 2017-03-27 Created: 2017-03-27 Last updated: 2018-03-01Bibliographically approved
Karlsson, O. & Roman, E. (2016). Dose-dependent effects of alcohol administration on behavioral profiles in the MCSF test.. Alcohol, 50, 51-56
Open this publication in new window or tab >>Dose-dependent effects of alcohol administration on behavioral profiles in the MCSF test.
2016 (English)In: Alcohol, ISSN 0741-8329, E-ISSN 1873-6823, Vol. 50, p. 51-56Article in journal (Refereed) Published
Abstract [en]

The acute effects of alcohol administration are age-, dose-, time- and task-dependent. Although generally considered to be a sedative drug, alcohol has both stimulatory and depressant effects on behavior, depending on dose and time. Alcohol-induced motor activating effects are consistently shown in mice but rarely demonstrated in adult, outbred rats using conventional behavioral tests. The aim of the present experiment was to study acute alcohol-induced effects on behavioral profiles in a more complex environment using the novel multivariate concentric square field™ (MCSF) test, designed for assessing different behaviors in the same trial including locomotor activity. Adult male Wistar rats (Sca:WI) were administered one intraperitoneal (i.p.) injection of alcohol (0.0 g/kg, 0.5 g/kg, 1.0 g/kg, or 1.5 g/kg) 5 min prior to the 30-min MCSF test. The two highest doses induced marked motor-suppressing effects. A significant interaction between group and time was found in general activity when comparing rats exposed to alcohol at 0.0 g/kg and 0.5 g/kg. In contrast to the 0.0 g/kg dose that increased the activity over time, animals administered the low dose (0.5 g/kg) demonstrated an initial high activity followed by a decline over time. No indications for acute alcohol-induced anxiolytic-like effects were found. The multivariate setting in the MCSF test appears to be sensitive for detecting motor-activating effects of low doses of alcohol as well as reduced locomotion at doses lower than in other behavioral tasks. The detection of subtle changes in behavior across time and dose is important for understanding alcohol-induced effects. This approach may be useful in evaluating alcohol doses that correspond to different degrees of intoxication in humans.

National Category
Neurosciences
Identifiers
urn:nbn:se:uu:diva-277708 (URN)10.1016/j.alcohol.2015.10.003 (DOI)000370770200007 ()26695588 (PubMedID)
Funder
Magnus Bergvall Foundation
Available from: 2016-02-22 Created: 2016-02-22 Last updated: 2018-01-10Bibliographically approved
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