uu.seUppsala University Publications
Change search
Link to record
Permanent link

Direct link
BETA
Zhou, Q
Alternative names
Publications (10 of 10) Show all publications
Rhodin, A., Gröndbladh, A., Ginya, H., NIlsson, K. W., Rosenblad, A., Zhou, Q., . . . Nyberg, F. (2013). Combined analysis of circulating β-endorphin with gene polymorphisms in OPRM1, CACNAD2 and ABCB1 reveals correlation with pain, opioid sensitivity and opioid-related side effects. Molecular Brain, 6, 8
Open this publication in new window or tab >>Combined analysis of circulating β-endorphin with gene polymorphisms in OPRM1, CACNAD2 and ABCB1 reveals correlation with pain, opioid sensitivity and opioid-related side effects
Show others...
2013 (English)In: Molecular Brain, ISSN 1756-6606, Vol. 6, p. 8-Article in journal (Refereed) Published
Abstract [en]

Background

Opioids are associated with wide inter-individual variability in the analgesic response and a narrow therapeutic index. This may be partly explained by the presence of single nucleotide polymorphisms (SNPs) in genes encoding molecular entities involved in opioid metabolism and receptor activation. This paper describes the investigation of SNPs in three genes that have a functional impact on the opioid response: OPRM1, which codes for the μ-opioid receptor; ABCB1 for the ATP-binding cassette B1 transporter enzyme; and the calcium channel complex subunit CACNA2D2. The genotyping was combined with an analysis of plasma levels of the opioid peptide β-endorphin in 80 well-defined patients with chronic low back pain scheduled for spinal fusion surgery, and with differential sensitivity to the opioid analgesic remifentanil. This patient group was compared with 56 healthy controls.

Results

The plasma β-endorphin levels were significantly higher in controls than in pain patients.

A higher incidence of opioid-related side effects and sex differences was found in patients with the minor allele of the ABCB1 gene. Further, a correlation between increased opioid sensitivity and the major CACNA2D2 allele was confirmed. A tendency of a relationship between opioid sensitivity and the minor allele of OPRM1 was also found.

Conclusions

Although the sample cohort in this study was limited to 80 patients it appears that it was possible to observe significant correlations between polymorphism in relevant genes and various items related to pain sensitivity and opioid response. Of particular interest is the new finding of a correlation between increased opioid sensitivity and the major CACNA2D2 allele. These observations may open for improved strategies in the clinical treatment of chronic pain with opioids.

Keywords
chronic pain, opioid sensitivity, beta-endorphin, mu-1-opioid receptor (OPRM1), calcium channel subunit 2 (CACNA2D2), ATP-binding cassette B1 (ABCB1)
National Category
Anesthesiology and Intensive Care Medical and Health Sciences Neurosciences
Research subject
Anaesthesiology
Identifiers
urn:nbn:se:uu:diva-129623 (URN)10.1186/1756-6606-6-8 (DOI)000316319900001 ()23402298 (PubMedID)
Available from: 2010-08-19 Created: 2010-08-19 Last updated: 2018-01-12
Zhou, Q., Carlsson, A., Hallberg, M. & Nyberg, F. (2011). Substance P N-terminal fragment SP(1-7) attenuates chronic morphine tolerance and affects dynorphin B and nociceptin in rats. Peptides, 32(8), 1661-1665
Open this publication in new window or tab >>Substance P N-terminal fragment SP(1-7) attenuates chronic morphine tolerance and affects dynorphin B and nociceptin in rats
2011 (English)In: Peptides, ISSN 0196-9781, E-ISSN 1873-5169, Vol. 32, no 8, p. 1661-1665Article in journal (Refereed) Published
Abstract [en]

The N-terminal substance P fragment SP(1-7) is known to modulate hyperalgesia and opioid withdrawal in animal models. This study examined the effects of intraperitoneal (i.p.) injections of SP(1-7) on chronic morphine tolerance and on the levels of dynorphin B (DYN B) and nociceptin/orphanin FQ(N/OFQ) in various brain areas of male Sprague Dawley rats. Morphine tolerance was induced by subcutaneous injections of the opioid (10 mg/kg) twice daily for 7 days. SP(1-7) injected i.p. (185 nmol/kg) 30 min prior to morphine reduced the development of morphine tolerance. Immunoreactive (it) DYN B and N/OFQ peptide levels were measured in several areas of the central nervous system. Levels of ir DYN B in rats treated with SP(1-7) and morphine were decreased in the nucleus accumbens. substantia nigra and ventral tegmental area and increased in the frontal cortex. The ir N/OFQ levels were increased in the periaqueductal gray and decreased in the nucleus accumbens. Since the concentration profiles of the two peptides were altered by SP(1-7) in the areas that are implicated in the modulation of opioid tolerance and analgesia, it is suggested that DYN B and N/OFQ systems may be involved in the effects of SP(1-7) on opioid tolerance.

Keywords
Dynorphin B (DYN B), Morphine, Nociceptin/orphanin FQ (N/OFQ), Opioid tolerance, Rat brain, Substance P (SP), SP(1-7)
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-159258 (URN)10.1016/j.peptides.2011.06.030 (DOI)000294572800014 ()
Available from: 2011-09-26 Created: 2011-09-26 Last updated: 2017-12-08Bibliographically approved
Rossbach, U., Nilsson, A., Fälth, M., Kultima, K., Zhou, Q., Hallberg, M., . . . Nyberg, F. (2009). A quantitative peptidomic analysis of peptides related to the endogenous opioid and tachykinin systems in nucleus accumbens of rats following naloxone-precipitated morphine withdrawal. Journal of Proteome Research, 8(2), 1091-1098
Open this publication in new window or tab >>A quantitative peptidomic analysis of peptides related to the endogenous opioid and tachykinin systems in nucleus accumbens of rats following naloxone-precipitated morphine withdrawal
Show others...
2009 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 8, no 2, p. 1091-1098Article in journal (Refereed) Published
Abstract [en]

We have applied a recently developed label-free mass spectrometry based peptidomic approach to identify and quantify a variety of endogenous peptides from rat nucleus accumbens following withdrawal in naloxone-precipitated, morphine-dependent rats of two separate strains. We focused on maturated, partially processed and truncated peptides derived from the peptide precursors proenkephalin, prodynorphin and preprotachykinin. The expression of several identified peptides was dependent on strain and was affected during morphine withdrawal.

Keywords
Peptidome, rat brain, Morphine, Opioid peptides, Tachykinins, Withdrawal
National Category
Neurosciences
Research subject
Neuroscience
Identifiers
urn:nbn:se:uu:diva-108890 (URN)10.1021/pr800669g (DOI)000263193300070 ()19159213 (PubMedID)
Available from: 2009-10-02 Created: 2009-10-02 Last updated: 2019-04-29Bibliographically approved
Gustafsson, L., Zhou, Q. & Nylander, I. (2007). Ethanol-induced effects on opioid peptides in adult male Wistar rats are dependent on early environmental factors. Neuroscience, 146(3), 1137-1149
Open this publication in new window or tab >>Ethanol-induced effects on opioid peptides in adult male Wistar rats are dependent on early environmental factors
2007 (English)In: Neuroscience, ISSN 0306-4522, E-ISSN 1873-7544, Vol. 146, no 3, p. 1137-1149Article in journal (Refereed) Published
Abstract [en]

The vulnerability to develop alcoholism is dependent on both genetic and environmental factors. The neurobiological mechanisms underlying these factors are not fully understood but individual divergence in the endogenous opioid peptide system may contribute. We have previously reported that early-life experiences can affect endogenous opioids and also adult voluntary ethanol intake. In the present study, this line of research was continued and the effects of long-term voluntary ethanol drinking on the opioid system are described in animals reared in different environmental settings. Rat pups were subjected to 15 min (MS15) or 360 min (MS360) of daily maternal separation during postnatal days 1–21. At 10 weeks of age, male rats were exposed to voluntary ethanol drinking in a four-bottle paradigm with 5%, 10% and 20% ethanol solution in addition to water for 2 months. Age-matched controls received water during the same period. Immunoreactive (ir) Met-enkephalin-Arg6Phe7 (MEAP) and dynorphin B (DYNB) peptide levels were thereafter measured in the pituitary gland and several brain areas. In water-drinking animals, lower ir MEAP levels were observed in the MS360 rats in the hypothalamus, medial prefrontal cortex, striatum and the periaqueductal gray, whereas no differences were seen in ir DYNB levels. Long-term ethanol drinking induced lower ir MEAP levels in MS15 rats in the medial prefrontal cortex and the periaqueductal gray, whereas higher levels were detected in MS360 rats in the hypothalamus, striatum and the substantia nigra. Chronic voluntary drinking affected ir DYNB levels in the pituitary gland, hypothalamus and the substantia nigra, with minor differences between MS15 and MS360. In conclusion, manipulation of the early environment caused changes in the opioid system and a subsequent altered response to ethanol. The altered sensitivity of the opioid peptides to ethanol may contribute to the previously reported differences in ethanol intake between MS15 and MS360 rats.

Keywords
handling, maternal separation, maternal deprivation, dynorphin B, MEAP, alcohol
National Category
Pharmaceutical Sciences
Identifiers
urn:nbn:se:uu:diva-95649 (URN)10.1016/j.neuroscience.2007.02.037 (DOI)000246584500023 ()17391858 (PubMedID)
Available from: 2007-03-30 Created: 2007-03-30 Last updated: 2018-01-13Bibliographically approved
Le Greves, M., Zhou, Q., Berg, M., Le Greves, P., Fholenhag, K., Meyerson, B. & Nyberg, F. (2006). Growth hormone replacement in hypophysectomized rats affects spatial performance and hippocampal levels of NMDA receptor subunit and PSD-95 gene transcript levels.. Exp Brain Res, 173(2), 267-73
Open this publication in new window or tab >>Growth hormone replacement in hypophysectomized rats affects spatial performance and hippocampal levels of NMDA receptor subunit and PSD-95 gene transcript levels.
Show others...
2006 (English)In: Exp Brain Res, ISSN 0014-4819, Vol. 173, no 2, p. 267-73Article in journal (Refereed) Published
Keywords
Animals, Blotting; Northern, Growth Hormone/*pharmacology, Hippocampus/*metabolism, Hormone Replacement Therapy, Hypophysectomy, Intracellular Signaling Peptides and Proteins, Male, Maze Learning/drug effects, Membrane Proteins/*biosynthesis, Memory/drug effects, Psychomotor Performance/drug effects, Rats, Rats; Sprague-Dawley, Receptors; N-Methyl-D-Aspartate/*metabolism, Space Perception/*physiology, Transcription; Genetic/drug effects, Weight Gain/drug effects
Identifiers
urn:nbn:se:uu:diva-25314 (URN)16633806 (PubMedID)
Available from: 2007-02-12 Created: 2007-02-12 Last updated: 2011-01-11
Botros, M., Hallberg, M., Johansson, T., Zhou, Q., Lindeberg, G., Frändberg, P., . . . Nyberg, F. (2005). Endomorphin-1 and endomorphin-2 differentially interact with specific binding sites for substance P (SP) aminoterminal SP(1-7) in the rat spinal cord.. Peptides
Open this publication in new window or tab >>Endomorphin-1 and endomorphin-2 differentially interact with specific binding sites for substance P (SP) aminoterminal SP(1-7) in the rat spinal cord.
Show others...
2005 (English)In: Peptides, ISSN 0196-9781Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-75676 (URN)16216386 (PubMedID)
Available from: 2006-02-14 Created: 2006-02-14 Last updated: 2011-01-11
Zhou, Q., Kindlundh, A., Hallberg, M. & Nyberg, F. (2004). The substance P (SP) heptapeptide fragment SP1-7 alters the density of dopamine receptors in rat brain mesocorticolimbic structures during morphine withdrawal. Peptides, 25(11), 1951-1957
Open this publication in new window or tab >>The substance P (SP) heptapeptide fragment SP1-7 alters the density of dopamine receptors in rat brain mesocorticolimbic structures during morphine withdrawal
2004 (English)In: Peptides, ISSN 0196-9781, E-ISSN 1873-5169, Vol. 25, no 11, p. 1951-1957Article in journal (Refereed) Published
Abstract [en]

The aminoterminal fragment of substance P (SP), SP(1-7), has been suggested to modulate the expression of opiate tolerance and withdrawal behaviors in rodents. However, the mechanism of this effect is not yet clarified. Using a rat model we have previously demonstrated that SP(1-7) affects dopamine transmission and the expression of the dopamine D2-receptor gene transcript in the nucleus accumbens during naloxone precipitated morphine withdrawal. In the present study, we have applied autoradiography to investigate the effect of the heptapeptide on the binding of dopamine D1- and D2-receptors in mesocorticolimbic brain areas of male rats during morphine withdrawal. Morphine dependent animals were treated with an injection of SP(1-7) into the ventral tegmental area prior to naloxone challenge. The result indicated that the SP fragment elicited a significant decrease in specific binding to D1-like receptors in the caudate putamen, nucleus accumbens shell, nucleus accumbens core, substantia nigra and medial globus pallidus. Radioligand binding to dopamine D2-like receptors was also altered by SP(1-7). The heptapeptide induced a decreased density of these sites in the ventral tegmental area but an increased binding in the substantia nigra and the frontal cortex. The observed alterations in the D1- and D2-like receptor density could reflect activations in dopamine pathways associated with the above-mentioned brain regions. The result provides further evidence for the modulatory effect of SP(1-7) on dopamine systems during opioid withdrawal, suggesting the possible role for the heptapeptide to regulate morphine withdrawal reactions.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-89568 (URN)10.1016/j.peptides.2004.07.011 (DOI)15501527 (PubMedID)
Available from: 2001-11-22 Created: 2001-11-22 Last updated: 2017-12-14Bibliographically approved
Zhou, Q. & Nyberg, F. (2002). Injection of substance P (SP) N-terminal fragment SP(1-7) into the ventraltegmental area modulates the levels of nucleus accumbens dopamine anddihydroxyphenylacetic acid in male rats during morphine withdrawal.. Neurosci Lett, 320, 117
Open this publication in new window or tab >>Injection of substance P (SP) N-terminal fragment SP(1-7) into the ventraltegmental area modulates the levels of nucleus accumbens dopamine anddihydroxyphenylacetic acid in male rats during morphine withdrawal.
2002 (English)In: Neurosci Lett, Vol. 320, p. 117-Article in journal (Refereed) Published
Abstract [en]

The levels of beta-endorphin and Met-enkephalin-Arg-Phe (MEAP) immunoreactivity in various brain regions (including amygdala, cortex, hippocampus, hypothalmus, nucleus accumbens, pituitary and ventral tegmental area) were studied in male rats subjected to

Keywords
anabolic androgenic steroids (AAS); beta-endorphin; drug dependence; Met-enkephalin-Arg-Phe (MEAP); nandrolone decanoate; rat brain; reward system; ventral tegmental area (VTA); TESTOSTERONE REGULATION; ARCUATE NUCLEUS; DOPAMINE; DEPENDENCE; ABUSE; NEURON
Identifiers
urn:nbn:se:uu:diva-64691 (URN)
Note
Addresses: Johansson P, DEPT PHARMACEUT BIOSCI, DIV BIOL RES DRUG DEPENDENCE, POB 591, S-75124 UPPSALA, SWEDEN.Available from: 2005-05-26 Created: 2005-05-26 Last updated: 2011-01-13
Zhou, Q., Liu, Z., Ray, A., Huang, W., Karlsson, K. & Nyberg, F. (1998). Alteration in the brain content of substance P (1-7) during withdrawal in morphine-dependent rats. NEUROPHARMACOLOGY, 37(12), 1545-1552
Open this publication in new window or tab >>Alteration in the brain content of substance P (1-7) during withdrawal in morphine-dependent rats
Show others...
1998 (English)In: NEUROPHARMACOLOGY, ISSN 0028-3908, Vol. 37, no 12, p. 1545-1552Article in journal (Other scientific) Published
Abstract [en]

Previous studies have shown that substance P (SP) may modulate the abstinence reaction to opioid withdrawal. Its N-terminal fragment SP1-7 may inhibit the intensity of the withdrawal reactions in morphine dependent mice, This study was designed to determi

Keywords
morphine; dependence; withdrawal; substance P; substance P N-terminal fragment; rat brain; HUMAN CEREBROSPINAL-FLUID; CONVERTING ENZYME-ACTIVITIES; VENTRAL TEGMENTAL AREA; CENTRAL-NERVOUS-SYSTEM; GENE-RELATED PEPTIDE; MOUSE SPINAL-CORD; NUCLEUS-ACCUMBENS;
Identifiers
urn:nbn:se:uu:diva-79031 (URN)
Note
Addresses: Nyberg F, Uppsala Univ, Dept Pharmaceut Biosci, Div Biol Res Drug Dependence, POB 591, S-75124 Uppsala, Sweden. Uppsala Univ, Dept Pharmaceut Biosci, Div Biol Res Drug Dependence, S-75124 Uppsala, Sweden.Available from: 2005-05-26 Created: 2005-05-26 Last updated: 2011-01-14
Johansson, P., Ray, A., Zhou, Q., Huang, W., Karlsson, K. & Nyberg, F. (1997). Anabolic androgenic steroids increase beta-endorphin levels in the ventral tegmental area in the male rat brain. NEUROSCIENCE RESEARCH, 27(2), 185-189
Open this publication in new window or tab >>Anabolic androgenic steroids increase beta-endorphin levels in the ventral tegmental area in the male rat brain
Show others...
1997 (English)In: NEUROSCIENCE RESEARCH, ISSN 0168-0102, Vol. 27, no 2, p. 185-189Article in journal (Refereed) Published
Abstract [en]

The levels of beta-endorphin and Met-enkephalin-Arg-Phe (MEAP) immunoreactivity in various brain regions (including amygdala, cortex, hippocampus, hypothalmus, nucleus accumbens, pituitary and ventral tegmental area) were studied in male rats subjected to

Keywords
anabolic androgenic steroids (AAS); beta-endorphin; drug dependence; Met-enkephalin-Arg-Phe (MEAP); nandrolone decanoate; rat brain; reward system; ventral tegmental area (VTA); TESTOSTERONE REGULATION; ARCUATE NUCLEUS; DOPAMINE; DEPENDENCE; ABUSE; NEURON
Identifiers
urn:nbn:se:uu:diva-79722 (URN)
Note
Addresses: Johansson P, DEPT PHARMACEUT BIOSCI, DIV BIOL RES DRUG DEPENDENCE, POB 591, S-75124 UPPSALA, SWEDEN.Available from: 2005-05-26 Created: 2005-05-26 Last updated: 2011-01-15
Organisations

Search in DiVA

Show all publications