uu.seUppsala University Publications
Change search
Link to record
Permanent link

Direct link
BETA
Wingqvist, Gunilla
Alternative names
Publications (10 of 22) Show all publications
Anderson, H., Wingqvist, G., Weissbach, T., Wallinder, D., Katardjiev, I. & Ingemarsson, B. (2011). Systematic investigation of biomolecular interactions using combined frequency and motional resistance measurements. Sensors and actuators. B, Chemical, 153(1), 135-144
Open this publication in new window or tab >>Systematic investigation of biomolecular interactions using combined frequency and motional resistance measurements
Show others...
2011 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 153, no 1, p. 135-144Article in journal (Refereed) Published
Abstract [en]

The resonance frequency of acoustic biosensors is today used as a label-free technique for detecting mass changes on sensor surfaces. In combination with an appropriate continuous flow system it has earlier been used for affinity and kinetic rate determination. Here, we assess the potential of a modified acoustic biosensor, monitoring also the real-time dissipation through the resistance of the sensor, to obtain additional kinetic information related to the structure and conformation of the molecules on the surface. Actual interaction studies, including an attempt to determine avidity, are presented along with thorough verification of the experimental setup utilizing true viscous load exposure together with protein and DNA immobilizations. True viscous loads show a linear relationship between resistance and frequency as expected. However, in the interaction studies between antibodies and proteins, as well as in the immobilization of DNA and proteins, higher surface concentrations of interacting molecules led to a decrease (i.e. deviation from the linear trend) in the differential resistance to frequency ratio. This is interpreted as increased surface rigidity at higher surface concentrations of immobilized molecules. Consequently, studies that aim at obtaining biological binding information, such as avidity, from real-time resistance and dissipation data should be conducted at low surface concentrations. In addition, the differential resistance to frequency relationship was found to be highly dependent on the rigidity of the preceding layer(s) of immobilized molecules. This dependence can be utilized to obtain a higher signal-to-noise ratio for resistance measurement by using low surface densities of immobilized interaction partners.

Keywords
biosensor, interaction analysis, QCM, dissipation, motional resistance, kinetics
National Category
Analytical Chemistry Other Industrial Biotechnology
Research subject
Analytical Chemistry; Engineering Science with specialization in Microsystems Technology; Engineering Science with specialization in Electronics
Identifiers
urn:nbn:se:uu:diva-107253 (URN)10.1016/j.snb.2010.10.019 (DOI)000289019300020 ()
Available from: 2009-07-30 Created: 2009-07-30 Last updated: 2018-06-26Bibliographically approved
Wingqvist, G., Arapan, L., Yantchev, V. & Katardjiev, I. (2009). Micromachined thermally compensated thin film Lamb wave resonator for frequency control and sensing applications. Journal of Micromechanics and Microengineering, 19(3), 035018
Open this publication in new window or tab >>Micromachined thermally compensated thin film Lamb wave resonator for frequency control and sensing applications
2009 (English)In: Journal of Micromechanics and Microengineering, ISSN 0960-1317, E-ISSN 1361-6439, Vol. 19, no 3, p. 035018-Article in journal (Refereed) Published
Abstract [en]

Micromachined thin film plate acoustic wave resonators (FPAR) utilizing the lowest order symmetric Lamb wave (S0) propagating in highly textured 2μm thick Aluminum Nitride (AlN) membranes have been successfully demonstrated [1]. The proposed devices have a SAW-based design and exhibit Q factors of up to 3000 at a frequency around 900MHz as well as design flexibility with respect to the required motional resistance. However, a notable drawback of the proposed devices is non-zero temperature coefficient of frequency (TCF) which lies in the range -20 ppm/K to –25 ppm/K. Thus, despite the promising features demonstrated, further device optimization is required. In this work temperature compensation of thin AlN film Lamb wave resonators is studied and experimentally demonstrated. Temperature compensation while retaining at the same time the device electromechanical coupling is experimentally demonstrated. The zero TCF Lamb wave resonators are fabricated onto composite AlN/SiO2 membranes. Q factors of around 1400 have been measured at a frequency of around 755 MHz. Finally, the impact of technological issues on the device performance is discussed in view of improving the device performance.

National Category
Other Electrical Engineering, Electronic Engineering, Information Engineering
Research subject
Electronics
Identifiers
urn:nbn:se:uu:diva-89404 (URN)10.1088/0960-1317/19/3/035018 (DOI)000263678200019 ()
Projects
WISENET
Funder
Swedish Research Council, 2009-5056
Available from: 2009-02-12 Created: 2009-02-12 Last updated: 2018-06-26Bibliographically approved
Wingqvist, G., Andersson, H., Lennartsson, C., Yantchev, V. & Lloyd Spetz, A. (2009). On the applicability of high frequency acoustic shear mode biosensing in view of thickness limitations set by the film resonance. Biosensors & bioelectronics, 24(11), 3387-3390
Open this publication in new window or tab >>On the applicability of high frequency acoustic shear mode biosensing in view of thickness limitations set by the film resonance
Show others...
2009 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 24, no 11, p. 3387-3390Article in journal (Refereed) Published
Abstract [en]

The IC-compatible thin film bulk acoustic resonator (FBAR) technology has made it possible to move the thickness excited shear mode sensing of biological layers into a new sensing regime using substantially higher operation frequencies than the conventionally used Quartz Crystal Microbalance (QCM). The limitations of the linear range set by the film resonance using viscoelastic protein films are here for the first time addressed specifically for FBARs operating at 700MHz up to 1.5GHz. Two types of protein multilayer sensing were employed; one utilizing alternating layers of Streptavidin and Biotinated BSA and the other using stepwise cross-linking of fibrinogen with EDC/NHS activation of its carboxyl groups. In both cases the number of protein layers within the linear regime is well above the number of protein layers usually used in biosensor applications, further verifying the applicability of the FBAR as a biosensor. Theoretical calculations are also presented using well established physical models to illustrate the expected behavior of the FBAR sensor, in view of both the frequency and the dissipation shifts.

Keywords
Shear mode electroacoustic sensing, Streptavidin–biotinated BSA, Fibrinogen, High frequency, Thin film bulk acoustic resonator (FBAR)
National Category
Engineering and Technology
Research subject
Electronics
Identifiers
urn:nbn:se:uu:diva-89406 (URN)10.1016/j.bios.2009.04.021 (DOI)000267577900035 ()
Available from: 2009-02-12 Created: 2009-02-12 Last updated: 2018-06-26Bibliographically approved
Kubart, T., Zhao, S., Wingqvist, G., Nyberg, T., Wäckelgård, E. & Berg, S. (2008). A model of DC reactive magnetron sputtering for graded solar thermal absorbers. Journal of Physics, Conference Series, 100(8), 082024
Open this publication in new window or tab >>A model of DC reactive magnetron sputtering for graded solar thermal absorbers
Show others...
2008 (English)In: Journal of Physics, Conference Series, ISSN 1742-6588, E-ISSN 1742-6596, Vol. 100, no 8, p. 082024-Article in journal (Refereed) Published
Abstract [en]

In this work we present process modelling of reactive in-line sputtering. Although modelling of reactive sputtering is often used, most models are based on assumptions which make them insufficient for use in this case. The presented model takes into account a realistic deposition rate profile and distribution of reactive gas pressure. The model has been applied to reactive sputter deposition of graded solar thermal absorbers. It has been shown that the presented model represents a simple alternative to complex Monte Carlo simulations while still representing all important features of the process.

National Category
Physical Sciences Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-87611 (URN)10.1088/1742-6596/100/8/082024 (DOI)´000275655200302 ()
Available from: 2009-01-02 Created: 2009-01-02 Last updated: 2018-06-26
Wingqvist, G., Yantchev, V. & Katardjiev, I. (2008). Mass sensitivity of multilayer thin film resonant BAW sensors. Sensors and Actuators A-Physical, 148(1), 88-95
Open this publication in new window or tab >>Mass sensitivity of multilayer thin film resonant BAW sensors
2008 (English)In: Sensors and Actuators A-Physical, ISSN 0924-4247, E-ISSN 1873-3069, Vol. 148, no 1, p. 88-95Article in journal (Refereed) Published
Abstract [en]

Abstract: A systematic study of the mass sensitivity and its dependence on the material's properties and thicknesses in composite multilayer Thin Film Bulk Acoustic Resonators (FBAR) is presented. The Mason transmission line model has been employed in combination with the acoustic energy balance principle for the determination of the FBAR mass sensitivity. The results have been experimentally verified. Further, the mass sensitivity dependence on various parameters has been studied and correlated with wave reflection and interference within the composite structure in addition to the well-known dependence on resonator acoustic impedance and operation frequency. The mass sensitivity for both the fundamental and the second harmonic mode of operation has been studied in view of their practical relevance. In particular, sensitivity amplification induced by the presence of an on-top deposited low acoustic impedance layer has been identified for the first harmonic and its potential applicability discussed in terms of gas and in-liquid sensing. Optimized structures for both sensing applications are suggested by considering the overall sensor resolution defined by both the mass sensitivity and the FBAR performance.

Keywords
Mass sensitivity, Bulk acoustic resonator, FBAR, Sensitivity amplification
National Category
Other Electrical Engineering, Electronic Engineering, Information Engineering
Research subject
Electronics
Identifiers
urn:nbn:se:uu:diva-86872 (URN)10.1016/j.sna.2008.07.023 (DOI)000260804500015 ()
Projects
WISENET
Available from: 2008-12-09 Created: 2008-12-08 Last updated: 2018-06-26Bibliographically approved
Wingqvist, G., Arapan, L., Yanchev, V. & Katardjiev, I. (2008). Temperature Compensation of Thin Film Resonators utilizing the Lowest order Symmetric Lamb mode. In: Proc. 2008 Int. IEEE Ultrason. Symp., 2008 Beijing, China: . Paper presented at IEEE International Ultrasonic Symposium.
Open this publication in new window or tab >>Temperature Compensation of Thin Film Resonators utilizing the Lowest order Symmetric Lamb mode
2008 (English)In: Proc. 2008 Int. IEEE Ultrason. Symp., 2008 Beijing, China, 2008Conference paper, Published paper (Refereed)
National Category
Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-142871 (URN)
Conference
IEEE International Ultrasonic Symposium
Projects
SSF-MS2EWISENET
Available from: 2011-01-17 Created: 2011-01-17 Last updated: 2018-06-26Bibliographically approved
Wingqvist,, G., Arapan, L. & Yantchev, V. (2008). Temperature Compensation of Thin Film Resonators utilizing the Lowest order Symmetric Lamb mode. In: : . Paper presented at IEEE Int. Ultrasonic Symposia, Beijing, China (2008). USA: IEEE
Open this publication in new window or tab >>Temperature Compensation of Thin Film Resonators utilizing the Lowest order Symmetric Lamb mode
2008 (English)Conference paper, Published paper (Other academic)
Abstract [en]

Micromachined Thin film plate acoustic wave resonators (FPAR) utilizing the lowest order symmetric Lamb wave (S0) propagating in highly textured 2μm thick Aluminum Nitride (AlN) membranes have been successfully demonstrated[1]. However, a notable drawback of the proposed devices is their non-zero temperature coefficient of frequency (TCF) which lies in the range -20 ppm/K to –25 ppm/K. In this work temperature compensation of thin AlN film Lamb wave resonators is studied and demonstrated. Temperature compensation, while retaining at the same time the device electromechanical coupling, isexperimentally demonstrated. The zero TCF Lamb wave resonators are fabricated onto composite AlN/SiO2 membranes. Q factors of around 1400 have been measured at a frequency of around 755 MHz.

Place, publisher, year, edition, pages
USA: IEEE, 2008
National Category
Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-88222 (URN)
Conference
IEEE Int. Ultrasonic Symposia, Beijing, China (2008)
Note

In print

Available from: 2009-01-26 Created: 2009-01-26 Last updated: 2018-06-26Bibliographically approved
Wingqvist, G., Yantchev, V., Bjurström, J. & Katardjiev, I. (2008). Thin film bulk acoustic resonators (FBARs) for biosensor applications. In: : . Paper presented at 7th International Workshop on Micromachined Ultrasonic Transducers, Trondheim, May 8-9.
Open this publication in new window or tab >>Thin film bulk acoustic resonators (FBARs) for biosensor applications
2008 (English)Conference paper, Published paper (Refereed)
National Category
Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-111004 (URN)
Conference
7th International Workshop on Micromachined Ultrasonic Transducers, Trondheim, May 8-9
Projects
WISENET
Available from: 2009-12-01 Created: 2009-12-01 Last updated: 2018-06-26Bibliographically approved
Wingqvist, G., Bjurström, J., Hellgren, A.-C. & Katardjiev, I. (2007). Immunosensor utilizing a shear mode thin film bulk acoustic sensor. Sensors and actuators. B, Chemical, 127(1), 248-252
Open this publication in new window or tab >>Immunosensor utilizing a shear mode thin film bulk acoustic sensor
2007 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 127, no 1, p. 248-252Article in journal (Refereed) Published
Abstract [en]

An AlN thin film electro-acoustic resonator has been fabricated employing a reactive sputtering process for the deposition of an AlN thin film with inclined c-axis for excitation of the shear mode for operation in liquid media. The main objective is to investigate the efficiency of the micro-fluidic channel system integrated in the silicon wafer underneath the AlN resonator. A comparative study between the shear mode thin film bulk acoustic resonator (FBAR) and a quartz crystal microbalance (QCM) using a competitive antibody–antigen association process for detection of drug molecules is presented.

Keywords
Competitive immunosensor, Shear mode FBAR, Drug detection
National Category
Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-11757 (URN)10.1016/j.snb.2007.07.051 (DOI)000250691100039 ()
Available from: 2007-10-16 Created: 2007-10-16 Last updated: 2017-12-11Bibliographically approved
Wingqvist, G., Yantchev, V., Bjurström, J. & Katardjiev, I. (2007). Mass Sensitivity of Thin Film Resonator Device. In: : . Paper presented at IEEE Freq Control Symp, Geneva, Switzerland, May 29 - June 1, 2007.
Open this publication in new window or tab >>Mass Sensitivity of Thin Film Resonator Device
2007 (English)Conference paper, Published paper (Refereed)
National Category
Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-138679 (URN)
Conference
IEEE Freq Control Symp, Geneva, Switzerland, May 29 - June 1, 2007
Projects
WISENET
Available from: 2010-12-17 Created: 2010-12-17 Last updated: 2018-06-26Bibliographically approved
Organisations

Search in DiVA

Show all publications