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Söderhäll, Irene
Alternative names
Publications (10 of 65) Show all publications
Ericsson, L. & Söderhäll, I. (2018). Astakines in arthropods-phylogeny and gene structure. Developmental and Comparative Immunology, 81, 141-151
Open this publication in new window or tab >>Astakines in arthropods-phylogeny and gene structure
2018 (English)In: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 81, p. 141-151Article in journal (Refereed) Published
Abstract [en]

Astakinel was isolated as a hematopoietic cytokine in the freshwater crayfish Pacifastacus leniusculus. In this study we detect and compare 79 sequences in GenBank, which we consider to be possible astakine orthologs, among which eleven are crustacean, sixteen are chelicerate and 52 are from insect species. Available arthropod genomes are searched for astakines, and in conclusion all astakine sequences in the current study have a similar exon containing CCXX(X), thus potentially indicating that they are homologous genes with the structure of this exon highly conserved. Two motifs, RYS and YP(N), are also conserved among the arthropod astakines. A phylogenetic analysis reveals that astakinel and astakine2 from P. leniusculus and Procambarus clarkii are distantly related, and may have been derived from a gene duplication occurring early in crustacean evolution. Moreover, a structural comparison using the Mamba intestinal toxin (MITI) from Dendroaspis polylepis as template indicates that the overall folds are similar in all crustacean astakines investigated.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD, 2018
Keywords
Astakine, Astakine-like, Prokineticin
National Category
Biological Sciences
Identifiers
urn:nbn:se:uu:diva-347534 (URN)10.1016/j.dci.2017.11.005 (DOI)000424726900014 ()29154857 (PubMedID)
Available from: 2018-04-04 Created: 2018-04-04 Last updated: 2018-04-04Bibliographically approved
Junkunlo, K., Söderhäll, K. & Söderhäll, I. (2018). Clotting protein: An extracellular matrix (ECM) protein involved in crustacean hematopoiesis. Developmental and Comparative Immunology, 78, 132-140
Open this publication in new window or tab >>Clotting protein: An extracellular matrix (ECM) protein involved in crustacean hematopoiesis
2018 (English)In: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 78, p. 132-140Article in journal (Refereed) Published
Abstract [en]

Hematopoietic progenitor cells in crustaceans are organized in lobule-like structures surrounded by different types of cells and extracellular matrix (ECM) proteins in a Hematopoietic tissue (HPT). Here we show that the clotting protein (CP) is part of the ECM in HPT and is secreted during HPT cell culture. The formation of a filamentous network of CP was observed in HPT cell culture. A high amount of CP protein was detected at the surfaces of undifferentiated cells (round-shaped) compared with migrating cells (spindle shaped). Co-localization of the CP protein and TGase activity was observed on the cell surface and filamentous network between cells. A role for CP together with collagen was revealed in a 3D culture in which a collagen-I matrix was immobilized with CP or supplemented with CP. The results showed possible functions of CP, collagen, TGase and the cytokine Ast1 in the regulation of HPT progenitor cell behavior. This is the first study to provide insight into the role of CP, which probably not only participates in clot formation but also functions as an ECM component protein controlling hematopoietic stem cell behavior.

Keywords
Clotting protein, ECM, Hematopoiesis, Crustacean
National Category
Zoology
Identifiers
urn:nbn:se:uu:diva-340667 (URN)10.1016/j.dci.2017.09.017 (DOI)000413881000014 ()28943319 (PubMedID)
Funder
Swedish Research Council, 621-2012-2416
Available from: 2018-02-05 Created: 2018-02-05 Last updated: 2018-02-05Bibliographically approved
Guo, E., Korkut, G. G., Jaree, P., Söderhäll, I. & Söderhäll, K. (2017). A Pacifastacus leniusculus serine protease interacts with WSSV. Fish and Shellfish Immunology, 68, 211-219
Open this publication in new window or tab >>A Pacifastacus leniusculus serine protease interacts with WSSV
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2017 (English)In: Fish and Shellfish Immunology, ISSN 1050-4648, E-ISSN 1095-9947, Vol. 68, p. 211-219Article in journal (Refereed) Published
Abstract [en]

Serine proteases are involved in many critical physiological processes including virus spread and replication. In the present study, we identified a new clip-domain serine protease (PIcSP) in the crayfish Pacifastacus leniusculus hemocytes, which can interact with the White Spot Syndrome Virus (WSSV) envelope protein VP28. It was characterized by a classic clip domain with six strictly conserved Cys residues, and contained the conserved His-Asp-Ser (H-D-S)motif in the catalytic domain. Furthermore, signal peptide prediction revealed that it has a 16-residue secretion signal peptide. Tissue distribution showed that it was mainly located in P. leniusculus hemocytes, and its expression was increased in hemocytes upon WSSV challenge. In vitro knock down of PIcSP decreased both the expression of VP28 and the WSSV copy number in hematopoietic stem (HPT) cells. Accordingly, these data suggest that the new serine protease may be of importance for WSSV infection into hematopoietic cells.

Keywords
Hematopoietic tissue, Invertebrate, Serine protease, Virus, WSSV
National Category
Immunology
Identifiers
urn:nbn:se:uu:diva-335858 (URN)10.1016/j.fsi.2017.07.026 (DOI)000411299500022 ()28705723 (PubMedID)
Funder
Swedish Research Council, 621-2012-2418
Available from: 2018-01-24 Created: 2018-01-24 Last updated: 2018-01-24Bibliographically approved
Junkunlo, K., Söderhäll, K., Noonin, C. & Söderhäll, I. (2017). PDGF/VEGF-related receptor affects transglutaminase activity to control cell migration during crustacean hematopoiesis. Stem Cells and Development, 26(20), 1449-1459
Open this publication in new window or tab >>PDGF/VEGF-related receptor affects transglutaminase activity to control cell migration during crustacean hematopoiesis
2017 (English)In: Stem Cells and Development, ISSN 1547-3287, E-ISSN 1557-8534, Vol. 26, no 20, p. 1449-1459Article in journal (Refereed) Published
Abstract [en]

The platelet-derived growth factor (PDGF) receptor, a tyrosine kinase (TK) receptor whose ligand is PDGF, is crucial in the transduction of extracellular signals into cells and mediates numerous processes, such as cell proliferation, differentiation, survival, and migration. We demonstrate the important roles of a receptor TK related to the PDGF/VEGF family protein (PVR) in controlling hematopoietic progenitor cell migration by affecting extracellular transglutaminase (TGase) activity. Pl_PVR1, GenBank accession No. KY444650, is highly expressed in hemocytes and the hematopoietic tissue (HPT). Sunitinib malate was used to block the PVF/PVR downstream pathway in HPT cell culture. The addition of Sunitinib also caused the HPT cells to increase in size and begin spreading. An increase in extracellular TGase activity on the HPT cell membrane was observed in a dose-dependent manner after treatment with Sunitinib malate. The presence of crude Ast1 provided a combinatorial beneficial effect that enhanced the number of spreading cells after inhibition of the Pl_PVR downstream signaling cascade. In addition, an increased immunoreactivity for beta-tubulin and elongation of beta-tubulin filaments were found in Pl_PVR signaling-inhibited cells. The potential roles of PVF/PVR signaling in controlling progenitor cell activity during hematopoiesis in crayfish were investigated and discussed.

Keywords
PDGF/VEGF, hematopoiesis, Transglutaminase, Ast1, crayfish
National Category
Developmental Biology Cell Biology Immunology
Research subject
Biology with specialization in Molecular Biology
Identifiers
urn:nbn:se:uu:diva-327243 (URN)10.1089/scd.2017.0086 (DOI)000412919700001 ()28805145 (PubMedID)
Funder
Swedish Research Council, VR 2011-4797, VR 621-2012-2418
Available from: 2017-08-07 Created: 2017-08-07 Last updated: 2018-01-03Bibliographically approved
Sirikharin, R., Junkunlo, K., Söderhäll, K. & Söderhäll, I. (2017). Role of astakine1 in regulating transglutaminase activity. Developmental and Comparative Immunology, 76, 77-82
Open this publication in new window or tab >>Role of astakine1 in regulating transglutaminase activity
2017 (English)In: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 76, p. 77-82Article in journal (Refereed) Published
Abstract [en]

Transglutaminase (TGase) has been implicated in maintaining the undifferentiated stage of hematopoietic stem cells (HSC) in the crayfish Pacifastacus leniusculus. TGase activity has been reported to be regulated by astakine1, an essential crayfish cytokine for inducing new hemocyte synthesis in hematopoietic tissue (HPT). Here, the role of astakine1 in TGase activity regulation and clotting protein (CP) cross-linking was characterized. A reduction in TGase activity was observed by the addition of purified astakine1 in vitro for both endogenous crayfish TGase and a commercial purified guinea pig liver TGase. As a result, we observed that astakine1 inhibits TGase enzyme activity and acts as a non-competitive inhibitor for the TGase enzyme. Additionally, the clotting reaction was impaired in the presence of astakine1. A decrease in TGase-mediated crosslinking of ε(γ-glutamyl)-lysine bonds was also observed in the presence of astakine1. In conclusion, this study shows that astakine1 acts as an inhibitor of TGase activity and that it also affects CP cross-linking during crayfish hematopoiesis.

Place, publisher, year, edition, pages
Elsevier, 2017
Keywords
Astakine1, Clotting protein, Hematopoiesis, Transglutaminase activity
National Category
Developmental Biology Immunology Zoology
Research subject
Biology with specialization in Comparative Physiology
Identifiers
urn:nbn:se:uu:diva-327217 (URN)10.1016/j.dci.2017.05.015 (DOI)000407985100009 ()28528959 (PubMedID)
Funder
Swedish Research Council, VR 621-2012-2418
Available from: 2017-08-07 Created: 2017-08-07 Last updated: 2017-10-09Bibliographically approved
Apitanyasai, K., Noonin, C., Tassanakajon, A., Söderhäll, I. & Söderhäll, K. (2016). Characterization of a hemocyte homeostasis-associated-like protein (HHAP) in the freshwater crayfish Pacifastacus leniusculus. Fish and Shellfish Immunology, 58, 429-435
Open this publication in new window or tab >>Characterization of a hemocyte homeostasis-associated-like protein (HHAP) in the freshwater crayfish Pacifastacus leniusculus
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2016 (English)In: Fish and Shellfish Immunology, ISSN 1050-4648, E-ISSN 1095-9947, Vol. 58, p. 429-435Article in journal (Refereed) Published
Abstract [en]

Hemocyte homeostasis-associated-like protein (HHAP) in the freshwater crayfish Pacifastacus leniusculus has a distinct role from that of its homolog PmHHAP in the shrimp Penaeus monodon. Knockdown of PIHHAP in vitro using double-stranded RNA (dsRNA) had no effect on the cell morphology of hematopoietic tissue (HPT) cells. The total hemocyte number and caspase activity were unchanged after PIHHAP knockdown in vivo, in contrast to the results found in shrimp. Moreover, suppression of PIHHAP both in vitro and in vivo did not change the mRNA levels of some genes involved in hematopoiesis and hemocyte homeostasis. Interestingly, bacterial count and scanning electron microscope revealed that depletion of PIHHAP in intestine by RNAi resulted in higher number of bacteria in the crayfish intestine. Together, these results suggest that PIHHAP is not involved in hemocyte homeostasis in the crayfish P. leniusculus but appears to affect the bacterial number in the intestine through an unknown mechanism. Since PIHHAP has different functions from PmHHAP, we therefore named it HHAP-like protein.

Keywords
Pacifastacus leniusculus, Hemocyte homeostasis, RNA interference, Intestine, Aeromonas hydrophila
National Category
Immunology
Identifiers
urn:nbn:se:uu:diva-309995 (URN)10.1016/j.fsi.2016.09.038 (DOI)000387523100051 ()27663854 (PubMedID)
Funder
Swedish Research Council, 2012-2418
Available from: 2016-12-12 Created: 2016-12-09 Last updated: 2017-11-29Bibliographically approved
Söderhäll, I. (2016). Crustacean hematopoiesis. Developmental and Comparative Immunology, 58, 129-141
Open this publication in new window or tab >>Crustacean hematopoiesis
2016 (English)In: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 58, p. 129-141Article in journal (Refereed) Published
Abstract [en]

Crustacean hemocytes are important mediators of immune reactions, and the regulation of hemocyte homeostasis is of utmost importance for the health of these animals. This review discusses the current knowledge on the lineages, synthesis and differentiation of hemocytes in crustaceans. Hematopoietic tissues, their origins, and the regulation of hematopoiesis during molting, seasonal variation and infection are discussed. Furthermore, studies concerning the molecular regulation of hemocyte formation in crustaceans are also described, and the different lineages and their molecular markers are discussed and compared with several insect species. Signaling pathways and the regulation of hematopoiesis by transcription factors are typically conserved among these arthropods, whereas cytokines and growth factors are more variable and species specific. However, considering the great diversity among the crustaceans, one should be cautious in drawing general conclusions from studies of only a few species.

Keywords
Crustaceans, Hematopoiesis, Astakine, Hemocytes
National Category
Zoology
Research subject
Biology with specialization in Comparative Physiology
Identifiers
urn:nbn:se:uu:diva-289236 (URN)10.1016/j.dci.2015.12.009 (DOI)000372764400013 ()26721583 (PubMedID)
Funder
Lars Hierta Memorial FoundationSwedish Research Council, VR 2011-4797, PRJNA259594
Note

Special Issue: Hematopoiesis and Immunity

Available from: 2016-04-29 Created: 2016-04-29 Last updated: 2017-08-22Bibliographically approved
Söderhäll, I. (2015). Special Issue: Specific Immunity in Invertebrates. Developmental and Comparative Immunology, 48(2), 267-268
Open this publication in new window or tab >>Special Issue: Specific Immunity in Invertebrates
2015 (English)In: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 48, no 2, p. 267-268Article in journal, Editorial material (Other academic) Published
National Category
Immunology
Identifiers
urn:nbn:se:uu:diva-246835 (URN)10.1016/j.dci.2014.09.002 (DOI)000347362200001 ()25218978 (PubMedID)
Available from: 2015-03-11 Created: 2015-03-10 Last updated: 2017-12-04Bibliographically approved
Jearaphunt, M., Noonin, C., Jiravanichpaisal, P., Nakamura, S., Tassanakajon, A., Söderhäll, I. & Söderhäll, K. (2014). Caspase-1-like regulation of the proPO-system and role of ppA and caspase-1-like cleaved peptides from proPO in innate immunity. PLoS Pathogens, 10(4), e1004059
Open this publication in new window or tab >>Caspase-1-like regulation of the proPO-system and role of ppA and caspase-1-like cleaved peptides from proPO in innate immunity
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2014 (English)In: PLoS Pathogens, ISSN 1553-7366, E-ISSN 1553-7374, Vol. 10, no 4, p. e1004059-Article in journal (Refereed) Published
Abstract [en]

Invertebrates rely on innate immunity to respond to the entry of foreign microorganisms. One of the important innate immune responses in arthropods is the activation of prophenoloxidase (proPO) by a proteolytic cascade finalized by the proPO-activating enzyme (ppA), which leads to melanization and the elimination of pathogens. Proteolytic cascades play a crucial role in innate immune reactions because they can be triggered more quickly than immune responses that require altered gene expression. Caspases are intracellular proteases involved in tightly regulated limited proteolysis of downstream processes and are also involved in inflammatory responses to infections for example by activation of interleukin 1ß. Here we show for the first time a link between caspase cleavage of proPO and release of this protein and the biological function of these fragments in response to bacterial infection in crayfish. Different fragments from the cleavage of proPO were studied to determine their roles in bacterial clearance and antimicrobial activity. These fragments include proPO-ppA, the N-terminal part of proPO cleaved by ppA, and proPO-casp1 and proPO-casp2, the fragments from the N-terminus after cleavage by caspase-1. The recombinant proteins corresponding to all three of these peptide fragments exhibited bacterial clearance activity in vivo, and proPO-ppA had antimicrobial activity, as evidenced by a drastic decrease in the number of Escherichia coli in vitro. The bacteria incubated with the proPO-ppA fragment were agglutinated and their cell morphology was altered. Our findings show an evolutionary conserved role for caspase cleavage in inflammation, and for the first time show a link between caspase induced inflammation and melanization. Further we give a more detailed understanding of how the proPO system is regulated in time and place and a role for the peptide generated by activation of proPO as well as for the peptides resulting from Caspase 1 proteolysis.

National Category
Immunology
Identifiers
urn:nbn:se:uu:diva-229571 (URN)10.1371/journal.ppat.1004059 (DOI)000342033600027 ()24722332 (PubMedID)
Funder
Swedish Research Council
Available from: 2014-08-11 Created: 2014-08-11 Last updated: 2017-12-05Bibliographically approved
Benton, J., Kery, R., Li, J., Noonin, C., Söderhäll, I. & Beltz, B. (2014). Cells from the Immune System Generate Adult-Born Neurons in Crayfish. Developmental Cell, 30(3), 322-333
Open this publication in new window or tab >>Cells from the Immune System Generate Adult-Born Neurons in Crayfish
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2014 (English)In: Developmental Cell, ISSN 1534-5807, E-ISSN 1878-1551, Vol. 30, no 3, p. 322-333Article in journal (Refereed) Published
Abstract [en]

Neurogenesis is an ongoing process in the brains of adult decapod crustaceans. However, the first-generation precursors that produce adult-born neurons, which reside in a neurogenic niche, are not self-renewing in crayfish and must be replenished. The source of these neuronal precursors is unknown. Here, we report that adult-born neurons in crayfish can be derived from hemocytes. Following adoptive transfer of 5-ethynyl-2′-deoxyuridine (EdU)-labeled hemocytes, labeled cells populate the neurogenic niche containing the first-generation neuronal precursors. Seven weeks after adoptive transfer, EdU-labeled cells are located in brain clusters 9 and 10 (where adult-born neurons differentiate) and express appropriate neurotransmitters. Moreover, the number of cells composing the neurogenic niche in crayfish is tightly correlated with total hemocyte counts (THCs) and can be manipulated by raising or lowering THC. These studies identify hemocytes as a source of adult-born neurons in crayfish and demonstrate that the immune system is a key contributor to adult neurogenesis.

Place, publisher, year, edition, pages
Cell Press, 2014
National Category
Developmental Biology Immunology Zoology
Research subject
Immunology
Identifiers
urn:nbn:se:uu:diva-229781 (URN)10.1016/j.devcel.2014.06.016 (DOI)000340344600009 ()25117683 (PubMedID)
Funder
Swedish Research Council, 2011-4797
Available from: 2014-08-13 Created: 2014-08-13 Last updated: 2017-06-30Bibliographically approved
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