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BETA
Garcia Bennett, A E
Alternative names
Publications (10 of 68) Show all publications
Bondarenko, O., Torres, N. F., Kupferschmidt, N., Garcia-Bennett, A. & Fadeel, B. (2014). Cellular uptake of mesoporous silica particles is governed by activation state of macrophages. Paper presented at 50th Congress of the European-Societies-of-Toxicology, SEP 07-10, 2014, Edinburgh, SCOTLAND. Toxicology Letters, 229, S188-S188
Open this publication in new window or tab >>Cellular uptake of mesoporous silica particles is governed by activation state of macrophages
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2014 (English)In: Toxicology Letters, ISSN 0378-4274, E-ISSN 1879-3169, Vol. 229, p. S188-S188Article in journal, Meeting abstract (Refereed) Published
National Category
Pharmacology and Toxicology Engineering and Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-233965 (URN)10.1016/j.toxlet.2014.06.638 (DOI)000341134000591 ()
Conference
50th Congress of the European-Societies-of-Toxicology, SEP 07-10, 2014, Edinburgh, SCOTLAND
Available from: 2014-10-15 Created: 2014-10-13 Last updated: 2018-01-11Bibliographically approved
Kupferschmidt, N., Rahman Qazi, K., Kemi, C., Vallhov, H., Garcia-Bennett, A. E., Gabrielsson, S. & Scheynius, A. (2014). Mesoporous silica particles potentiate antigen specific T cell responses. Nanomedicine, 9(12), 1835-1846
Open this publication in new window or tab >>Mesoporous silica particles potentiate antigen specific T cell responses
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2014 (English)In: Nanomedicine, ISSN 1743-5889, E-ISSN 1748-6963, Vol. 9, no 12, p. 1835-1846Article in journal (Refereed) Published
National Category
Medical Biotechnology
Identifiers
urn:nbn:se:uu:diva-195901 (URN)10.2217/nnm.13.170 (DOI)000343904800006 ()
Available from: 2013-02-28 Created: 2013-02-28 Last updated: 2017-12-06Bibliographically approved
Garcia-Bennett, A. E., Kozhevnikova, M., König, N., Zhou, C., Leao, R., Knöpfel, T., . . . Kozlova, E. N. (2013). Delivery of Differentiation Factors by Mesoporous Silica Particles Assists Advanced Differentiation of Transplanted Murine Embryonic Stem Cells. Stem Cells Translational Medicine, 2(11), 906-915
Open this publication in new window or tab >>Delivery of Differentiation Factors by Mesoporous Silica Particles Assists Advanced Differentiation of Transplanted Murine Embryonic Stem Cells
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2013 (English)In: Stem Cells Translational Medicine, ISSN 2157-6564, E-ISSN 2157-6580, Vol. 2, no 11, p. 906-915Article in journal (Refereed) Published
Abstract [en]

Stem cell transplantation holds great hope for the replacement of damaged cells in the nervous system. However, poor long-term survival after transplantation and insufficiently robust differentiation of stem cells into specialized cell types in vivo remain major obstacles for clinical application. Here, we report the development of a novel technological approach for the local delivery of exogenous trophic factor mimetics to transplanted cells using specifically designed silica nanoporous particles. We demonstrated that delivering Cintrofin and Gliafin, established peptide mimetics of the ciliary neurotrophic factor and glial cell line-derived neurotrophic factor, respectively, with these particles enabled not only robust functional differentiation of motor neurons from transplanted embryonic stem cells but also their long-term survival in vivo. We propose that the delivery of growth factors by mesoporous nanoparticles is a potentially versatile and widely applicable strategy for efficient differentiation and functional integration of stem cell derivatives upon transplantation.

Keywords
Cell transplantation, Differentiation, Embryonic stem cells, Nervous system, Neural differentiation, Neural stem cell, Stem cell culture, Transplantation
National Category
Natural Sciences Engineering and Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-211443 (URN)10.5966/sctm.2013-0072 (DOI)000326312000017 ()
Note

De 3 första författarna delar förstaförfattarskapet

Available from: 2013-11-27 Created: 2013-11-25 Last updated: 2017-12-06Bibliographically approved
Kupferschmidt, N., Xia, X., Labrador, R. H., Atluri, R., Ballell, L. & Garcia-Bennett, A. E. (2013). In vivo oral toxicological evaluation of mesoporous silica particles. Nanomedicine, 8(1), 57-64
Open this publication in new window or tab >>In vivo oral toxicological evaluation of mesoporous silica particles
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2013 (English)In: Nanomedicine, ISSN 1743-5889, E-ISSN 1748-6963, Vol. 8, no 1, p. 57-64Article in journal (Refereed) Published
Abstract [en]

Background: Mesoporous silica particles are highly promising nanomaterials for biomedical applications. They can be used to improve bioavailability, solubility and drug stability and to protect drugs from the acidic conditions of the stomach, leading to increased drug effectiveness. Their biocompatibility in vivo has recieved little attention, in particular regarding oral administration. Aim: To study the oral tolerance of micron-sized nanoporous folic acid-templated material-1 (cylindrical, 2D hexagonal pore structure) and nanometer-sized anionic-surfactant-templated mesoporous silica material-6 (cylindrical, 3D cubic pore structure) mesoporous silica particles in Sprague Dawley rats. Materials & methods: A dose stepwise procedure or range finding test was followed by a consequent confirmatory test. The confirmatory test included daily administrations of 2000 and 1200 mg/kg doses for nanoporous folic acid-templated material-1 and anionic-surfactant-templated mesoporous silica material-6, respectively. Results: The maximum tolerated dose for anionic-surfactant-templated mesoporous silica material-6 was not reached. Similar results were observed for nanometer-sized anionic-surfactant-templated mesoporous silica material-1 in most of the animals, although adverse effects were observed in some animals that are most probably due to the administration by oral gavage of the formulated particles. Conclusion: The results are promising for the use of mesoporous silica materials as drug-delivery systems in oral administration.

National Category
Natural Sciences Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-180100 (URN)10.2217/nnm.12.77 (DOI)000314577800014 ()22891863 (PubMedID)
Available from: 2012-08-29 Created: 2012-08-29 Last updated: 2017-12-07Bibliographically approved
Kupferschmidt, N., Csikasz, R., Ballell, L., Bengtsson, T. & Garcia-Bennett, A. E. (2013). Large pore mesoporous silica induced weight loss in obese mice. Nanomedicine, 9(9), 1353-1362
Open this publication in new window or tab >>Large pore mesoporous silica induced weight loss in obese mice
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2013 (English)In: Nanomedicine, ISSN 1743-5889, E-ISSN 1748-6963, Vol. 9, no 9, p. 1353-1362Article in journal (Refereed) Published
Abstract [en]

Background: There is a need for medical treatments to curb the rising rate of obesity. Weight reduction is correlated with a decrease in associated risk factors and cholesterol levels in humans. Amorphous silica particles have been found to exert a hypocholesterolemic effect in humans, making them popular dietary additives. Aim: To investigate the effect of mesoporous silica, which possess sharp pore size distributions, on: weight loss, cholesterol, triglycerides and glucose blood levels in obese mice. Materials & methods: Mesoporous silicas with differing pore size were mixed in the high-fat diet of obese mice. Results: Animals receiving large pore mesoporous silica with a high-fat diet show a significant reduction in body weight and fat composition, with no observable negative effects. Conclusion: Pore size is an important parameter for reduction of body weight and body fat composition by mesoporous silica, demonstrating promising signs for the treatment of obesity.

Keywords
body fat, body weight, cholesterol, high-fat diet, mesoporous silica, obesity
National Category
Other Medical Biotechnology Engineering and Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-195903 (URN)10.2217/nnm.13.138 (DOI)000342071700012 ()24471500 (PubMedID)
Funder
Swedish Research Council, 2009-4716Novo NordiskSwedish Diabetes AssociationMagnus Bergvall FoundationCarl Tryggers foundation
Available from: 2013-02-28 Created: 2013-02-28 Last updated: 2017-07-05Bibliographically approved
Atluri, R., Iqbal, M. N., Bacsik, Z., Hedin, N., Villaescusa, L. A. & Garcia-Bennett, A. E. (2013). Self-assembly mechanism of folate-templated mesoporous silica.. Langmuir, 29(38), 12003-12
Open this publication in new window or tab >>Self-assembly mechanism of folate-templated mesoporous silica.
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2013 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 29, no 38, p. 12003-12Article in journal (Refereed) Published
Abstract [en]

A method to form ordered mesoporous silica based on the use of folate supramolecular templates has been developed. Evidence based on in situ small-angle X-ray scattering (SAXS), electron microscopy, infrared spectroscopy, and in situ conductivity measurements are used to investigate the organic-inorganic interactions and synthesis mechanism. The behavior of folate molecules in solution differs distinctively from that of surfactants commonly used for the preparation of ordered mesoporous silica phases, notably with the absence of a critical micellar concentration. In situ SAXS studies reveal fluctuations in X-ray scattering intensities consistent with the condensation of the silica precursor surrounding the folate template and the growth of the silica mesostructure in the initial stages. High-angle X-ray diffraction shows that the folate template is well-ordered within the pores even after a few minutes of synthesis. Direct structural data for the self-assembly of folates into chiral tetramers within the pores of mesoporous silica provide evidence for the in register stacking of folate tetramers, resulting in a chiral surface of rotated tetramers, with a rotation angle of 30°. Additionally, the self-assembled folates within pores were capable of adsorbing a considerable amount of CO2 gas through the cavity space of the tetramers. The study demonstrates the validity of using a naturally occurring template to produce relevant and functional mesoporous materials.

National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-211753 (URN)10.1021/la401532j (DOI)23971901 (PubMedID)
Available from: 2013-11-30 Created: 2013-11-30 Last updated: 2017-12-06Bibliographically approved
Vallhov, H., Kupferschmidt, N., Gabrielsson, S., Paulie, S., Strømme, M., Garcia-Bennett, A. E. & Scheynius, A. (2012). Adjuvant Properties of Mesoporous Silica Particles Tune the Development of Effector T Cells. Small, 8(13), 2116-2124
Open this publication in new window or tab >>Adjuvant Properties of Mesoporous Silica Particles Tune the Development of Effector T Cells
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2012 (English)In: Small, ISSN 1613-6810, Vol. 8, no 13, p. 2116-2124Article in journal (Refereed) Published
Abstract [en]

Alum is the most frequently used adjuvant today, primarily inducing Th2 responses. However, Th1-type responses are often desirable within immune therapy, and therefore the development of new adjuvants is greatly needed. Mesoporous silica particles with a highly ordered pore structure have properties that make them very interesting for future controlled drug delivery systems, such as controllable particle and pore size; they also have the ability to induce minor immune modulatory effects, as previously demonstrated on human-monocyte-derived dendritic cells (MDDCs). In this study, mesoporous silica particles are shown to be efficiently engulfed by MDDCs within 2 h, probably by phagocytic uptake, as seen by confocal microscopy and transmission electron microscopy. A co-culture protocol is developed to evaluate the capability of MDDCs to stimulate the development of naive CD4+ T cells in different directions. The method, involving ELISpot as a readout system, demonstrates that MDDCs, after exposure to mesoporous silica particles (AMS-6 and SBA-15), are capable of tuning autologous naive T cells into different effector cells. Depending on the size and functionalization of the particles added to the cells, different cytokine patterns are detected. This suggests that mesoporous silica particles can be used as delivery vehicles with tunable adjuvant properties, which may be of importance for several medical applications, such as immune therapy and vaccination.

Place, publisher, year, edition, pages
John Wiley & Sons, 2012
Keywords
adjuvants, dendritic cells, immunology, mesoporous silica particles, naive T cells
National Category
Engineering and Technology Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-179024 (URN)10.1002/smll.201102620 (DOI)000306011500021 ()
Available from: 2012-08-06 Created: 2012-08-06 Last updated: 2016-11-30Bibliographically approved
Kupferschmidt, N. & Garcia-Bennett, A. (2012). Biocompatibility and Immunological Evaluation of Mesoporous Silica for use as Potential Adjuvants.. In: Materials for Tomorrow. Paper presented at Materials for Tomorrow.
Open this publication in new window or tab >>Biocompatibility and Immunological Evaluation of Mesoporous Silica for use as Potential Adjuvants.
2012 (English)In: Materials for Tomorrow, 2012Conference paper, Published paper (Refereed)
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-183521 (URN)
Conference
Materials for Tomorrow
Available from: 2012-10-29 Created: 2012-10-29 Last updated: 2013-01-02
Xia, X., Zhou, C., Ballell, L. & Garcia-Bennett, A. E. (2012). In vivo Enhancement in Bioavailability of Atazanavir in the Presence of Proton-Pump Inhibitors using Mesoporous Materials. ChemMedChem, 7(1), 43-8
Open this publication in new window or tab >>In vivo Enhancement in Bioavailability of Atazanavir in the Presence of Proton-Pump Inhibitors using Mesoporous Materials
2012 (English)In: ChemMedChem, ISSN 1860-7179, E-ISSN 1860-7187, Vol. 7, no 1, p. 43-8Article in journal (Refereed) Published
Abstract [en]

Matters of the HAART! The current treatment for human immunodeficiency virus (HIV), HAART, makes use of a combination of antiretroviral drugs, which are poorly soluble in aqueous media. Enhancing the solubility of such drugs through the use of mesoporous materials could lead to improved treatment efficiency and might provide a solution to the drug-drug interaction problems associated with these types of therapeutic regimes.

Keywords
drug delivery, human immunodeficiency virus (HIV), mesoporous silica, proton-pump inhibitors, solubility
National Category
Pharmaceutical Biotechnology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials; Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-165287 (URN)10.1002/cmdc.201100500 (DOI)000298549100004 ()22144293 (PubMedID)
Funder
Swedish Research Council
Available from: 2012-01-04 Created: 2012-01-04 Last updated: 2017-12-08Bibliographically approved
Shi, J., Karlsson, H. L., Johansson, K., Gogvadze, V., Xiao, L., Li, J., . . . Fadeel, B. (2012). Microsomal Glutathione Transferase 1 Protects Against Toxicity Induced by Silica Nanoparticles but Not by Zinc Oxide Nanoparticles. ACS Nano, 6(3), 1925-1938
Open this publication in new window or tab >>Microsomal Glutathione Transferase 1 Protects Against Toxicity Induced by Silica Nanoparticles but Not by Zinc Oxide Nanoparticles
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2012 (English)In: ACS Nano, ISSN 1936-0851, E-ISSN 1936-086X, Vol. 6, no 3, p. 1925-1938Article in journal (Refereed) Published
Abstract [en]

Microsomal glutathione transferase 1 (MGST1) is an antioxidant enzyme located predominantly in the mitochondrial er membrane and endoplasmk reticulum and has been shown to protect cells from lipid peroxidation induced by a variety of cytostatic drugs and pro-oxidant stimuli. We hypothesized that MGST1 may also protect against nanomaterial-induced cytotoxicity through a specific effect on lipid peroxidation. We evaluated the induction of cytotoxicity and oxidative stress by TiO2, CeO2, SiO2, and ZnO in the human MCF-7 cell line with or without overexpression of MGST1. SiO2 and ZnO nanoparticles caused dose- and time-dependent toxicity, whereas no obvious cytotoxic effects were induced by nanoparticles of TiO2 and CeO2. We also noted pronounced cytotoxicity for three out of four additional SiO2 nanoparticles tested. Overexpression of MGST1 reversed the cytotoxicity of the main SiO2 nanoparticles tested and for one of the supplementary SiO2 nanoparticles but did not protect cells against ZnO-induced cytotoxic effects. The data point toward a role of lipid peroxidation In SiO2 nanoparticle-induced cell death. For ZnO nanoparticles, rapid dissolution was observed, and the subsequent interaction of Zn2+ with cellular targets is likely to contribute to the cytotoxic effects. A direct inhibition of MGST1 by Zn2+ could provide a possible explanation for the lack of protection against ZnO nanoparticles in this model. Our data also showed that SiO2 nanoparticle-induced cytotoxicity is mitigated in the presence of serum, potentially through masking of reactive surface groups by serum proteins, whereas ZnO nanoparticles were cytotoxic both In the presence and in the absence of serum.

Keywords
engineered nanoparticles, oxidative stress, lipid peroxidation, microsomal glutathione transferase 1
National Category
Engineering and Technology Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-173348 (URN)10.1021/nn2021056 (DOI)000301945900006 ()
Available from: 2012-04-24 Created: 2012-04-23 Last updated: 2017-12-07Bibliographically approved
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