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Brohede, Ulrika
Alternative names
Publications (10 of 27) Show all publications
Stenlund, P., Omar, O., Brohede, U., Norgren, S., Norlindh, B., Johansson, A., . . . Palmquist, A. (2015). Bone response to a novel Ti–Ta–Nb–Zr alloy. Acta Biomaterialia, 20, 165-175
Open this publication in new window or tab >>Bone response to a novel Ti–Ta–Nb–Zr alloy
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2015 (English)In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 20, p. 165-175Article in journal (Refereed) Published
National Category
Other Materials Engineering
Identifiers
urn:nbn:se:uu:diva-286427 (URN)
Available from: 2016-04-20 Created: 2016-04-20 Last updated: 2017-11-30
Stenlund, P., Omar, O., Brohede, U., Norgren, S., Norlindh, B., Johansson, A., . . . Palmquist, A. (2015). Bone response to a novel Ti-Ta-Nb-Zr alloy. Acta Biomaterialia, 20, 165-175
Open this publication in new window or tab >>Bone response to a novel Ti-Ta-Nb-Zr alloy
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2015 (English)In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 20, p. 165-175Article in journal (Refereed) Published
Abstract [en]

Commercially pure titanium (cp-Ti) is regarded as the state-of-the-art material for bone-anchored dental devices, whereas the mechanically stronger alloy (Ti-6Al-4V), made of titanium, aluminum (Al) and vanadium (V), is regarded as the material of choice for high-load applications. There is a call for the development of new alloys, not only to eliminate the potential toxic effect of Al and V but also to meet the challenges imposed on dental and maxillofacial reconstructive devices, for example. The present work evaluates a novel, dual-stage, acid-etched, Ti-Ta-Nb-Zr alloy implant, consisting of elements that create low toxicity, with the potential to promote osseointegration in vivo. The alloy implants (denoted Ti-Ta-Nb-Zr) were evaluated after 7 days and 28 days in a rat tibia model, with reference to commercially pure titanium grade 4 (denoted Ti). Analyses were performed with respect to removal torque, histomorphometry and gene expression. The Ti-Ta-Nb-Zr showed a significant increase in implant stability over time in contrast to the Ti. Further, the histological and gene expression analyses suggested faster healing around the Ti-Ta-Nb-Zr, as judged by the enhanced remodeling, and mineralization, of the early-formed woven bone and the multiple positive correlations between genes denoting inflammation, bone formation and remodeling. Based on the present experiments, it is concluded that the Ti-Ta-Nb-Zr alloy becomes osseointegrated to at least a similar degree to that of pure titanium implants. This alloy is therefore emerging as a novel implant material for clinical evaluation.

Keywords
Osseointegration, Removal torque, Gene expression, Titanium alloy, Tantalum (Ta)
National Category
Biomaterials Science Engineering and Technology
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-256981 (URN)10.1016/j.actbio.2015.03.038 (DOI)000355708200018 ()25848727 (PubMedID)
Available from: 2015-07-01 Created: 2015-06-29 Last updated: 2017-12-04Bibliographically approved
Lilja, M., Sörensen, J., Brohede, U., Åstrand, M., Procter, P., Arnoldi, J., . . . Strömme, M. (2013). Drug eluting hydroxyapatite coatings for biomedical applications. European Cells and Materials, 26(2), 36
Open this publication in new window or tab >>Drug eluting hydroxyapatite coatings for biomedical applications
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2013 (English)In: European Cells and Materials, ISSN 1473-2262, E-ISSN 1473-2262, ISSN 1473-2262, Vol. 26, no 2, p. 36-Article in journal (Refereed) Published
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-211696 (URN)
Available from: 2013-11-28 Created: 2013-11-28 Last updated: 2017-12-06Bibliographically approved
Lilja, M., Sörensen, J. H., Brohede, U., Åstrand, M., Procter, P., Arnoldi, J., . . . Strømme, M. (2013). Drug loading and release of Tobramycin from Hydroxyapatite coated fixation pins. Journal of materials science. Materials in medicine, 24(9), 2265-2274
Open this publication in new window or tab >>Drug loading and release of Tobramycin from Hydroxyapatite coated fixation pins
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2013 (English)In: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 24, no 9, p. 2265-2274Article in journal (Refereed) Published
Abstract [en]

This paper evaluates the loading and release properties of Tobramycin incorporated by adsorptive loading from a solution into plasma sprayed and biomimetically coated Hydroxyapatite (HA) fixation pins. The aim of this study is to contribute towards designing a functional implant surface offering local release of the antibiotic agent to prevent post-surgical infections. Cathodic arc deposition is used to coat stainless steel fixation pins with a bioactive, anatase phase dominated, TiO2 coating onto which a HA layer is grown biomimetically. The loading and release properties are evaluated by studying the subsequent release of Tobramycin using high performance liquid chromatography and correlated to the differences in HA coating microstructure and the physical conditions under loading. The results from these studies show that a dual loading strategy consisting of a solution temperature of 90 °C and a pressure of 6 bar during a loading time of 5 min release a sufficient amount of Tobramycin to guarantee the inhibition of Staphylococcus aureus up to 2 days for plasma sprayed HA coatings and for 8 days for biomimetic coatings. The present study emphasizes the advantages of the nanoporous structure of biomimetically deposited HA over the more dense structure of plasma sprayed HA coatings in terms of antibiotic incorporation and subsequent sustained release and provides a valuable outline for the design of implant surfaces aiming for a fast-loading and controlled, local drug administration.

National Category
Medical Materials
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-203448 (URN)10.1007/s10856-013-4979-1 (DOI)000323289600018 ()
Available from: 2013-07-10 Created: 2013-07-10 Last updated: 2017-12-06Bibliographically approved
Lilja, M., Sörensen, J., Brohede, U., Åstrand, M., Procter, P., Arnoldi, J., . . . Strömme, M. (2013). Drugeluting hydroxyapatite coatings for biomedical applications. In: Scandinavian Society for Biomaterials: . Paper presented at Scandinavian Society for Biomaterials, Hafjell, Norway, March2013.
Open this publication in new window or tab >>Drugeluting hydroxyapatite coatings for biomedical applications
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2013 (English)In: Scandinavian Society for Biomaterials, 2013Conference paper, Poster (with or without abstract) (Refereed)
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-211747 (URN)
Conference
Scandinavian Society for Biomaterials, Hafjell, Norway, March2013
Available from: 2013-11-30 Created: 2013-11-30 Last updated: 2016-11-30
Forsgren, J., Brohede, U., Strømme, M. & Engqvist, H. (2011). Co-loading of bisphosphonates and antibiotics to a biomimetic hydroxyapatite coating. Biotechnology letters, 33(6), 1265-1268
Open this publication in new window or tab >>Co-loading of bisphosphonates and antibiotics to a biomimetic hydroxyapatite coating
2011 (English)In: Biotechnology letters, ISSN 0141-5492, E-ISSN 1573-6776, Vol. 33, no 6, p. 1265-1268Article in journal (Refereed) Published
Abstract [en]

We have incorporated bisphosphonates and antibiotics simultaneously into a biomimetic hydroxyapatite implant coating aiming to use the interaction between drug-molecules and hydroxyapatite to enable local release of the two different substances to obtain a dual biological effect. A sustained release over for 43 h of antibiotics (cephalothin) was achieved without negative interference from the presence of the bisphosphonate (clodronate) which, in turn, successfully bonded to the coating surface. To our knowledge, this is the first study that indicates the possibility to simultaneously incorporate both antibiotics and bisphosphonates to an implant coating, a strategy that is believed to improve implant stability and reduce implant-related infections.

National Category
Nano Technology Medical Biotechnology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-145824 (URN)10.1007/s10529-011-0542-7 (DOI)000291655200027 ()21287232 (PubMedID)
Available from: 2011-02-11 Created: 2011-02-11 Last updated: 2018-02-08Bibliographically approved
Forsgren, J., Brohede, U., Piskounova, S., Mihranyan, A., Larsson, S., Maria, S. & Engqvist, H. (2011). In Vivo Evaluation of Functionalized Biomimetic Hydroxyapatite for Local Delivery of Active Agents. Journal of Biomaterials and Nanobiotechnology, 2(2), 149-154
Open this publication in new window or tab >>In Vivo Evaluation of Functionalized Biomimetic Hydroxyapatite for Local Delivery of Active Agents
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2011 (English)In: Journal of Biomaterials and Nanobiotechnology, ISSN 2158-7027, 2158-7043, Vol. 2, no 2, p. 149-154Article in journal (Refereed) Published
Abstract [en]

This study was carried out to investigate the biological response in vivo to biomimetic hydroxyapatite implant coatings functionalized with bisphosphonates and bone morphogenetic proteins. The functionalization was carried out by a simple soaking procedure in the operating room immediately prior to surgery. Cylindrical titanium samples with and without coatings were implanted in the distal femoral epiphysis of sheep and retrieved after 6 weeks. The histological analysis proved that all samples were integrated well in the tissue with no signs of intolerance. Fewer osteoclasts were observed in the vicinity of bisphosphonate-functionalized samples and the bone was denser around these samples compared to the other samples. Samples functionalized with bone morphogenetic protein induced more bone/implant contact but showed a more inconsistent outcome with reduced bone density around the samples. This study demonstrates a simple method to functionalize implant coatings, which provides surgeons with an option of patient-specific functionalization of implants. The observed biological impact due to the delivery of active molecules from the coatings suggests that this strategy may also be employed to deliver antibiotics from similar coatings.

National Category
Engineering and Technology Polymer Chemistry
Research subject
Engineering Science with specialization in Materials Science; Engineering Science with specialization in Nanotechnology and Functional Materials; Chemistry with specialization in Polymer Chemistry
Identifiers
urn:nbn:se:uu:diva-156129 (URN)10.4236/jbnb.2011.22019 (DOI)
Funder
Swedish Research Council
Available from: 2011-07-11 Created: 2011-07-11 Last updated: 2018-02-08Bibliographically approved
Brohede, U., Zhao, S., Lindberg, F., Mihranyan, A., Forsgren, J., Strömme, M. & Engqvist, H. (2009). A novel graded bioactive high adhesion implant coating. Applied Surface Science, 255(17), 7723-7728
Open this publication in new window or tab >>A novel graded bioactive high adhesion implant coating
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2009 (English)In: Applied Surface Science, ISSN 0169-4332, E-ISSN 1873-5584, Vol. 255, no 17, p. 7723-7728Article in journal (Refereed) Published
Abstract [en]

 One method to increase the clinical success rate of metal implants is to increase their bone bonding properties, i.e. to develop a bone   bioactive surface leading to reduced risks of interfacial problems.   Much research has been devoted to modifying the surface of metals to   make them become bioactive. Many of the proposed methods include   depositing a coating on the implant. However, there is a risk of coating failure due to low substrate adhesion. This paper describes a method to obtain bioactivity combined with a high coating adhesion via   a gradient structure of the coating. Gradient coatings were deposited   on Ti (grade 5) using reactive magnetron sputtering with increasing   oxygen content. To increase the grain size in the coating, all coatings   were post annealed at 385 degrees C. The obtained coating exhibited a gradual transition over 70 nm from crystalline titanium oxide (anatase)  at the surface to metallic Ti in the substrate, as shown using  cross-section transmission electron microscopy and X-ray photoelectron   spectroscopy depth pro. ling. Using scratch testing, it could be shown that the adhesion to the substrate was well above 1 GPa. The bioactivity of the coating was verified in vitro by the spontaneous   formation of hydroxylapatite upon storage in phosphate buffer solution at 37 degrees C for one week.   The described process can be applied to implants irrespective of bulk  metal in the base and should introduce the possibility to create safer permanent implants like reconstructive devices, dental, or spinal implants.

Keywords
Implants, Coatings, Titanium oxide, XPS, XRD, TEM, Bioactivity, Adhesion
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-102109 (URN)10.1016/j.apsusc.2009.04.149 (DOI)000266567400045 ()
Available from: 2009-05-05 Created: 2009-05-05 Last updated: 2018-02-08Bibliographically approved
Åberg, J., Brohede, U., Mihranyan, A., Strømme, M. & Engqvist, H. (2009). Bisphosphonate incorporation in surgical implant coatings by fast loading and co-precipitation at low drug concentrations. Journal of materials science. Materials in medicine, 20(10), 2053-2061
Open this publication in new window or tab >>Bisphosphonate incorporation in surgical implant coatings by fast loading and co-precipitation at low drug concentrations
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2009 (English)In: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 20, no 10, p. 2053-2061Article in journal (Refereed) Published
Abstract [en]

The objectives of the present work was to evaluate the possibility for fast loading by soaking of bisphosphonates (BPs) into hydroxylapatite (HA) implant coatings biomimetically grown on crystalline TiO(2) surfaces, and also investigate the influence of different BP loading concentrations in a buffer during co-precipitation of a calcium phosphate containing layer onto these surfaces. The co-precipitation method created coatings that contained BPs throughout most of the coating layer, but the presence of BPs in the buffer hindered the formation of a bulk HA-layer, thus resulting in very thin coatings most likely consisting of islands built up by a calcium phosphate containing BPs. The coatings biomimetically grown on TiO(2) surfaces, were shown to consist of crystalline HA. Soaking of these coatings during 15 min only in a low BPs concentration containing buffer yielded a concentration on the coating surface of the same order of magnitude as obtained with soaking during 60 min in significantly higher concentrated buffers. This could be of advantage during surgery since the operating surgeon could make a fast decision whether or not to include the drugs in the coating based on the need of the particular patient at hand. The BPs present on the surface of the fast-loaded HA coatings were found to be strongly bound, something which should be beneficial for in vivo use. Both the co-precipitation method and the fast loading by soaking method investigated here are promising techniques for loading of BPs onto surgical implants. The simplicity of both methods is an advantage since implants can have spatially complicated structures.

National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-103825 (URN)10.1007/s10856-009-3771-8 (DOI)000270201100010 ()19449201 (PubMedID)
Available from: 2009-05-21 Created: 2009-05-21 Last updated: 2018-02-08Bibliographically approved
Forsgren, J., Brohede, U., Mihranyan, A., Engqvist, H. & Strömme, M. (2009). Fast loading, slow release: a new strategy for incorporating antibiotics. Key Engineering Materials, 396-398, 523-526
Open this publication in new window or tab >>Fast loading, slow release: a new strategy for incorporating antibiotics
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2009 (English)In: Key Engineering Materials, ISSN 1662-9795, Vol. 396-398, p. 523-526Article in journal (Refereed) Published
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-17832 (URN)130.238.20.17-03/09/08,13:15:59 (DOI)
Available from: 2008-09-03 Created: 2008-09-03 Last updated: 2018-02-08Bibliographically approved
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