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Tian, B., Han, Y., Fock, J., Strömberg, M., Leifer, K. & Fougt Hansen, M. (2019). Self-Assembled Magnetic Nanoparticle−Graphene Oxide Nanotag for Optomagnetic Detection of DNA. Acs Applied Nano Materials, 2(3), pp. 1683-1690
Open this publication in new window or tab >>Self-Assembled Magnetic Nanoparticle−Graphene Oxide Nanotag for Optomagnetic Detection of DNA
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2019 (English)In: Acs Applied Nano Materials, ISSN 2574-0970, Vol. 2, no 3, p. 1683-1690Article in journal, News item (Refereed) Published
Abstract [en]

In this work, a two-dimensional self-assembled magnetic nanoparticle–graphene oxide (MNP-GO) nanocomposite is reported for the detection of DNA. Single-stranded DNA (ssDNA) coils, generated through a rolling circle amplification (RCA) reaction triggered by the hybridization of target oligos and padlock probes, have a strong interaction with MNP-GO nanotags through several mechanisms including π–π stacking, hydrogen bonding, van der Waals, electrostatic, and hydrophobic interactions. This interaction leads to a hydrodynamic size increase or aggregation of MNP-GO nanotags, which can be detected by a simple optomagnetic setup. Due to the high shape anisotropy, MNP-GO nanotags provide stronger optomagnetic signal than individual MNPs. Moreover, the avoidance of DNA probes (i.e., short ssDNA sequences as the biosensing receptor) provides easier material preparation and lower measurement cost. From real-time measurements of interactions between MNP-GO and RCA products amplified from a highly conserved Escherichia coli 16S rDNA sequence, a limit of detection of 2 pM was achieved with a total assay time of 90 min. Although the nonspecific binding force between GO and ssDNA is much weaker than the specific base-pairing force in a DNA duplex, the proposed method provides a detection limit similar to DNA probe-based magnetic biosensors, which can be ascribed to the abundant binding sites between GO and ssDNA. In addition, for target concentrations higher than 100 pM, the MNP-GO nanotags can be applied for a qualitative naked eye detection strategy based on nanotag–ssDNA flocculation.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019
Keywords
Magnetic nanoparticles; graphene oxide; rolling circle amplification; single stranded DNA detection; optomagnetic sensing
National Category
Nano Technology Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-378551 (URN)10.1021/acsanm.9b00127 (DOI)000462554000061 ()
Available from: 2019-03-06 Created: 2019-03-06 Last updated: 2019-04-24Bibliographically approved
Tian, B., Han, Y., Fock, J., Strömberg, M., Leifer, K. & Fougt Hansen, M. (2019). Self-Assembled Magnetic Nanoparticle−Graphene Oxide Nanotag for Optomagnetic Detection of DNA. Acs Applied Nano Materials, 2(3), 1683-1690
Open this publication in new window or tab >>Self-Assembled Magnetic Nanoparticle−Graphene Oxide Nanotag for Optomagnetic Detection of DNA
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2019 (English)In: Acs Applied Nano Materials, ISSN 2574-0970, Vol. 2, no 3, p. 1683-1690Article in journal (Refereed) Published
Abstract [en]

In this work, a two-dimensional self-assembled magnetic nanoparticle–graphene oxide (MNP-GO) nanocomposite is reported for the detection of DNA. Single-stranded DNA (ssDNA) coils, generated through a rolling circle amplification (RCA) reaction triggered by the hybridization of target oligos and padlock probes, have a strong interaction with MNP-GO nanotags through several mechanisms including π–π stacking, hydrogen bonding, van der Waals, electrostatic, and hydrophobic interactions. This interaction leads to a hydrodynamic size increase or aggregation of MNP-GO nanotags, which can be detected by a simple optomagnetic setup. Due to the high shape anisotropy, MNP-GO nanotags provide stronger optomagnetic signal than individual MNPs. Moreover, the avoidance of DNA probes (i.e., short ssDNA sequences as the biosensing receptor) provides easier material preparation and lower measurement cost. From real-time measurements of interactions between MNP-GO and RCA products amplified from a highly conserved Escherichia coli 16S rDNA sequence, a limit of detection of 2 pM was achieved with a total assay time of 90 min. Although the nonspecific binding force between GO and ssDNA is much weaker than the specific base-pairing force in a DNA duplex, the proposed method provides a detection limit similar to DNA probe-based magnetic biosensors, which can be ascribed to the abundant binding sites between GO and ssDNA. In addition, for target concentrations higher than 100 pM, the MNP-GO nanotags can be applied for a qualitative naked eye detection strategy based on nanotag–ssDNA flocculation.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019
Keywords
Magnetic nanoparticles; graphene oxide; rolling circle amplification; single stranded DNA detection; optomagnetic sensing
National Category
Nano Technology Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-378551 (URN)10.1021/acsanm.9b00127 (DOI)000462554000061 ()
Available from: 2019-03-06 Created: 2019-04-24 Last updated: 2019-04-24Bibliographically approved
Tian, B., Liao, X., Svedlindh, P., Strömberg, M. & Wetterskog, E. (2018). Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA. ACS Sensors, 3(6), 1093-1101
Open this publication in new window or tab >>Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA
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2018 (English)In: ACS Sensors, ISSN 2379-3694, Vol. 3, no 6, p. 1093-1101Article in journal (Refereed) Published
Abstract [en]

The ability to detect and analyze the state ofmagnetic labels with high sensitivity is of crucial importance fordeveloping magnetic biosensors. In this work, we demonstrate, forthefirst time, a ferromagnetic resonance (FMR) basedhomogeneous and volumetric biosensor for magnetic labeldetection. Two different isothermal amplification methods, i.e.,rolling circle amplification (RCA) and loop-mediated isothermalamplification (LAMP), are adopted and combined with a standardelectron paramagnetic resonance (EPR) spectrometer for FMRbiosensing. For the RCA-based FMR biosensor, binding of RCAproducts of a syntheticVibrio choleraetarget DNA sequence givesrise to the formation of aggregates of magnetic nanoparticles.Immobilization of nanoparticles within the aggregates leads to adecrease of the net anisotropy of the system and a concomitant increase of the resonancefield. A limit of detection of 1 pM isobtained with a linear detection range between 7.8 and 250 pM. For the LAMP-based sensing, a synthetic Zika virus targetoligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by theircoprecipitation with Mg2P2O7(a byproduct of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement,high sensitivity, and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate fordesigning future point-of-care devices.

National Category
Condensed Matter Physics Engineering and Technology
Research subject
Engineering Science with specialization in Solid State Physics
Identifiers
urn:nbn:se:uu:diva-345595 (URN)10.1021/acssensors.8b00048 (DOI)000436525800005 ()
Funder
Swedish Research Council Formas, 221-2012-444Swedish Research Council Formas, 2011-1692EU, FP7, Seventh Framework Programme, FP7-NMP-601118
Available from: 2018-03-09 Created: 2018-03-09 Last updated: 2019-04-24Bibliographically approved
Tian, B., Svedlindh, P., Strömberg, M. & Wetterskog, E. (2018). Ferromagnetic resonance biosensor for homogeneous and volumetric detection of DNA. In: : . Paper presented at Biosensors conference, 12 - 15 June 2018, Miami, United States. , Article ID Abstract No. 0244.
Open this publication in new window or tab >>Ferromagnetic resonance biosensor for homogeneous and volumetric detection of DNA
2018 (English)Conference paper, Oral presentation with published abstract (Refereed)
National Category
Condensed Matter Physics
Research subject
Engineering Science with specialization in Solid State Physics
Identifiers
urn:nbn:se:uu:diva-363319 (URN)
Conference
Biosensors conference, 12 - 15 June 2018, Miami, United States
Funder
Swedish Research Council Formas, 221-2012-444Swedish Research Council Formas, 2011-1692EU, FP7, Seventh Framework Programme, FP7-NMP-604448
Available from: 2018-10-16 Created: 2018-10-16 Last updated: 2019-04-24Bibliographically approved
Wang, J., Xu, C., Nilsson, A. M., Fernandes, D. L. A., Strömberg, M., Wang, J. & Niklasson, G. (2018). General Method for Determining Light Scattering and Absorption of Nanoparticle Composites. Advanced Optical Materials, 6(4), Article ID 1801315.
Open this publication in new window or tab >>General Method for Determining Light Scattering and Absorption of Nanoparticle Composites
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2018 (English)In: Advanced Optical Materials, ISSN 2162-7568, E-ISSN 2195-1071, Vol. 6, no 4, article id 1801315Article in journal (Refereed) Published
Abstract [en]

Scattering and absorption from nanoparticles are of major importance in optical research as well as in a range of applications. The Kubelka–Munk two-flux radiative transfer model gives a simple description of light scattering in nanoparticle composite materials, but inversion of experimental transmittance and reflectance data to obtain backscattering and absorption coefficients remains challenging. Here, a general method for evaluating these parameters from transmittance and reflectance spectra, combined with spectral angle resolved light scattering measurements is developed. The angular dependence is approximatedby an extension of the empirical Reynolds–McCormick phase function, which is fitted to the experimental angle resolved light scattering data. This approach is verified by measurements on three typical nanoparticle/polymer composites containing plasmonic Au, ferromagnetic Fe3O4, and dielectric TiO2 particles. An approximation to the angular scattering pattern is further demonstrated, which can be applied to obtain the optical parameters using only reflectance and transmittance data, in cases where angle-resolved measurements are not available. These results can be extended to a wide range of isotropic, anisotropic, and multiple scattering systems, and will be highly useful in the fields of light scattering coatings/metamaterials, UV-shielding films, displays, absorption/scattering layers in solar cells and biological scatterers.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2018
National Category
Other Physics Topics Condensed Matter Physics
Research subject
Engineering Science with specialization in Solid State Physics
Identifiers
urn:nbn:se:uu:diva-370103 (URN)10.1002/adom.201801315 (DOI)000459020300005 ()
Funder
Swedish Research Council, 2016-03713Swedish Research Council Formas, 221-2012-444
Available from: 2018-12-19 Created: 2018-12-19 Last updated: 2019-08-01Bibliographically approved
Tian, B., Han, Y., Wetterskog, E., Donolato, M., Fougt Hansen, M., Svedlindh, P. & Strömberg, M. (2018). MicroRNA Detection through DNAzyme-Mediated Disintegration of Magnetic Nanoparticle Assemblies. ACS Sensors, 3, 1884-1891
Open this publication in new window or tab >>MicroRNA Detection through DNAzyme-Mediated Disintegration of Magnetic Nanoparticle Assemblies
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2018 (English)In: ACS Sensors, ISSN 2379-3694, Vol. 3, p. 1884-1891Article in journal (Refereed) Published
Abstract [en]

DNA-assembled nanoparticle superstructures offer numerous bioresponsive properties that can be utilized for point-of-care diagnostics. Functional DNA sequences such as deoxyribozymes (DNAzymes) provide novel bioresponsive strategies and further extend the application of DNA-assembled nanoparticle superstructures. In this work, we describe a microRNA detection biosensor that combines magnetic nanoparticle (MNP) assemblies with DNAzyme-assisted target recycling. The DNA scaffolds of the MNP assemblies contain substrate sequences for DNAzyme and can form cleavage catalytic structures in the presence of target DNA or RNA sequences, leading to rupture of the scaffolds and disintegration of the MNP assemblies. The target sequences are preserved during the cleavage reaction and release into the suspension to trigger the digestion of multiple DNA scaffolds. The high local concentration of substrate sequences in the MNP assemblies reduces the diffusion time for target recycling. The concentration of released MNPs, which is proportional to the concentration of the target, can be quantified by a 405 nm laser-based optomagnetic sensor. For the detection of let-7b in 10% serum, after 1 h of isothermal reaction at 50 degrees C, we found a linear detection range between 10 pM and 100 nM with a limit of detection of 6 pM. For the quantification of DNA target in buffer solution, a limit of detection of 1.5 pM was achieved. Compared to protein enzyme-based microRNA detection methods, the proposed DNAzyme-based biosensor has an increased stability, a reduced cost and a possibility to be used in living cells, all of which are valuable features for biosensing applications.

National Category
Analytical Chemistry Engineering and Technology
Research subject
Engineering Science with specialization in Solid State Physics
Identifiers
urn:nbn:se:uu:diva-363317 (URN)10.1021/acssensors.8b00850 (DOI)000446276300038 ()30188122 (PubMedID)
Funder
Swedish Research Council Formas, 221-2012-444Swedish Research Council Formas, 2011-1692EU, FP7, Seventh Framework Programme, 604448-NanoMag
Available from: 2018-10-16 Created: 2018-10-16 Last updated: 2019-04-24Bibliographically approved
Tian, B., Qiu, Z., Ma, J., Donolato, M., Fougt Hansen, M., Svedlindh, P. & Strömberg, M. (2018). On-Particle Rolling Circle Amplification-Based Core-Satellite Magnetic Superstructures for MicroRNA Detection. ACS Applied Materials and Interfaces, 10(3), 2957-2964
Open this publication in new window or tab >>On-Particle Rolling Circle Amplification-Based Core-Satellite Magnetic Superstructures for MicroRNA Detection
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2018 (English)In: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 10, no 3, p. 2957-2964Article in journal (Refereed) Published
Abstract [en]

Benefiting from the specially tailored properties of the building blocks as well as of the scaffolds, DNA-assembled core satellite superstructures have gained increasing interest-in drug delivery, imaging, and biosensing. The load of satellites plays,,a vital role in core satellite superstructures, and it determines the signal intensity in response to a biological/physical stimulation/actuation. Herein, for the first time, we utilize on-particle rolling circle amplification (RCA) to prepare rapidly responsive-core satellite magnetic superstructures With a high load of magnetic nanoparticle (MNP) Satellites. Combined with duplex-specific nuclease-assisted target recycling) the proposed magnetic superstructures hold great promise in sensitive and rapid microRNA detection. The long single-stranded DNA produced by RCA serving as the scaffold of the core satellite superstructure can be hydrolyzed by duplex-Specific nuclease in the presence of target microRNA, resulting in a release of MNPs that can be quantified in an optomagnetic sensor. The proposed biosensor has a-simple mix separate measure strategy. For let-7b detection, the proposed biosensor offers a wide linear detection range of approximately 5 orders of magnitude with a detection sensitivity of 1 fM. Moreover, it has the capability to discriminate single-nucleotide mismatches and to detect let-7b in cell extracts and serum, thus showing considerable potential for clinical applications.

National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Solid State Physics
Identifiers
urn:nbn:se:uu:diva-345594 (URN)10.1021/acsami.7b16293 (DOI)000423496500087 ()29266917 (PubMedID)
Funder
Swedish Research Council Formas, 221-2012-444
Available from: 2018-03-09 Created: 2018-03-09 Last updated: 2019-04-24Bibliographically approved
Tian, B., Qiu, Z., Ma, J., Zardán Gómez de la Torre, T., Donolato, M., Fougt Hansen, M., . . . Strömberg, M. (2018). Optomagnetic detection of microRNA based on duplex-specific nuclease assisted targetrecycling and core-satellite magnetic superstructures. In: : . Paper presented at Biosensors 2018 conference. , Article ID Abstract No. 0242.
Open this publication in new window or tab >>Optomagnetic detection of microRNA based on duplex-specific nuclease assisted targetrecycling and core-satellite magnetic superstructures
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2018 (English)Conference paper, Poster (with or without abstract) (Refereed)
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Solid State Physics
Identifiers
urn:nbn:se:uu:diva-363320 (URN)
Conference
Biosensors 2018 conference
Funder
Swedish Research Council Formas, 221-2012-444Swedish Research Council Formas, 2011-1692EU, FP7, Seventh Framework Programme, 604448-NanoMag
Available from: 2018-10-16 Created: 2018-10-16 Last updated: 2019-04-24
Fock, J., Parmvi, M., Strömberg, M., Svedlindh, P., Donolato, M. & Fougt Hansen, M. (2017). Comparison of optomagnetic and AC susceptibility readouts in a magnetic nanoparticle agglutination assay for detection of C-reactive protein. Biosensors & bioelectronics, 88, 94-100
Open this publication in new window or tab >>Comparison of optomagnetic and AC susceptibility readouts in a magnetic nanoparticle agglutination assay for detection of C-reactive protein
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2017 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 88, p. 94-100Article in journal (Refereed) Published
Abstract [en]

There is an increasing need to develop biosensor methods that are highly sensitive and, that can be combined with low-cost consumables. The use of magnetic nanoparticles (MNPs) is attractive because their detection is compatible with low-cost disposables and because application of a magnetic field can be used to accelerate assay kinetics. We present the first study and comparison of the performance of magnetic susceptibility measurements and a newly proposed optomagnetic method. For the comparison we use the C-reactive protein (CRP) induced agglutination of identical samples of 100 nm MNPs conjugated with CRP antibodies. Both methods detect agglutination as a shift to lower frequencies in measurements of the dynamics in response to an applied oscillating magnetic field. The magnetic susceptibility method probes the magnetic response whereas the optomagnetic technique probes the modulation of laser light transmitted through the sample. The two techniques provided highly correlated results upon agglutination when they measure the decrease of the signal from the individual MNPs (turn-off detection strategy), whereas the techniques provided different results, strongly depending on the read-out frequency, when detecting the signal due to MNP agglomerates (turn-on detection strategy). These observations are considered to be caused by differences in the volume-dependence of the magnetic and optical signals from agglomerates. The highest signal from agglomerates was found in the optomagnetic signal at low frequencies.

Place, publisher, year, edition, pages
Elsevier, 2017
Keywords
Brownian relaxation, CRP, Agglutination assay, Magnetic beads, Biosensor
National Category
Other Engineering and Technologies
Research subject
Engineering Science with specialization in Solid State Physics
Identifiers
urn:nbn:se:uu:diva-305173 (URN)10.1016/j.bios.2016.07.088 (DOI)000389112700014 ()27488263 (PubMedID)
Funder
Swedish Research Council Formas, 221-2012-444
Available from: 2016-10-12 Created: 2016-10-12 Last updated: 2019-04-24Bibliographically approved
Tian, B., Ma, J., Qiu, Z., Gómez de la Torre, T. Z., Donolato, M., Hansen, M. F., . . . Strömberg, M. (2017). Optomagnetic Detection of MicroRNA Based on Duplex-Specific Nuclease-Assisted Target Recycling and Multilayer Core-Satellite Magnetic Superstructures. ACS Nano, 11(2), 1798-1806
Open this publication in new window or tab >>Optomagnetic Detection of MicroRNA Based on Duplex-Specific Nuclease-Assisted Target Recycling and Multilayer Core-Satellite Magnetic Superstructures
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2017 (English)In: ACS Nano, ISSN 1936-0851, E-ISSN 1936-086X, Vol. 11, no 2, p. 1798-1806Article in journal (Refereed) Published
Abstract [en]

Superstructural assembly of magnetic nanoparticles enables approaches to biosensing by combining specially tailored properties of superstructures and the particular advantages associated with a magnetic or optomagnetic read-out such as low background signal, easy manipulation, cost-efficiency, and potential for bioresponsive multiplexing. Herein, we demonstrate a sensitive and rapid miRNA detection method based on optomagnetic read-out, duplex-specific nuclease (DSN)-assisted target recycling, and the use of multilayer core-satellite magnetic superstructures. Triggered by the presence of target miRNA and DSN-assisted target recycling, the core-satellite magnetic superstructures release their "satellites" to the suspension, which subsequently can be quantified accurately in a lowcost and user-friendly optomagnetic setup. Target miRNAs are preserved in the cleaving reaction and can thereby trigger more cleavage and release of "satellites". For singleplex detection of let-7b, a linear detection range between 10 fM and 10 nM was observed, and a detection limit of 4.8 fM was obtained within a total assay time of 70 min. Multiplexing was achieved by releasing nanoparticles of different sizes in the presence of different miRNAs. The proposed method also has the advantages of single-nucleotide mismatch discrimination and the ability of quantification in a clinical sample matrix, thus holding great promise for miRNA routine multiplex diagnostics.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2017
Keywords
miRNA detection, duplex-specific nuclease, magnetic nanoparticles, core-satellite superstructures, optomagnetic bioassay
National Category
Biomaterials Science Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-319101 (URN)10.1021/acsnano.6b07763 (DOI)000395357300073 ()28177611 (PubMedID)
Funder
Swedish Research Council Formas, 221-2012-444 221-2014-574 2011-1692
Available from: 2017-04-03 Created: 2017-04-03 Last updated: 2019-04-24Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-0648-3130

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