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Höglund, Anna-Stina
Publications (4 of 4) Show all publications
Karlsson, P., Lindblad, J., Bengtsson, E., Höglund, A.-S., Liu, J. & Larsson, L. (2007). Analysis of Skeletal Fibers in Three Dimensional Images. In: Medicinteknikdagarna 2007 (pp. 1).
Open this publication in new window or tab >>Analysis of Skeletal Fibers in Three Dimensional Images
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2007 (English)In: Medicinteknikdagarna 2007, 2007, p. 1-Conference paper, Published paper (Other (popular science, discussion, etc.))
National Category
Computer Vision and Robotics (Autonomous Systems)
Identifiers
urn:nbn:se:uu:diva-12575 (URN)
Available from: 2008-01-10 Created: 2008-01-10 Last updated: 2018-08-24Bibliographically approved
Karlsson, P., Lindblad, J., Bengtsson, E., Höglund, A.-S., Liu, J. & Larsson, L. (2007). Analysis of Skeletal Fibers in Three Dimensional Images: Methodological considerations. In: XXXVIth European Muscle Conference of the European Society for Muscle Research: European Muscle Conference 2007 (pp. 130).
Open this publication in new window or tab >>Analysis of Skeletal Fibers in Three Dimensional Images: Methodological considerations
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2007 (English)In: XXXVIth European Muscle Conference of the European Society for Muscle Research: European Muscle Conference 2007, 2007, p. 130-Conference paper, Published paper (Other academic)
Abstract [en]

Knowledge of the detailed three dimensional organization of nuclei in skeletal muscle fibers is of fundamental importance for the understanding of the basic mechanisms involved in muscle wasting associated with for example neuromuscular disorders and aging. An ongoing interdisciplinary collaboration between the Centre for Image Analysis (CBA), and the Muscle Research Group (MRG), both at Uppsala University, addresses the issue of spatial distribution of myonuclei using confocal microscopic techniques together with advanced methods for computerized image analysis.

Performing quantitative analysis on true three dimensional volume images captured by confocal microscopy gives us the option to perform in-depth statistical analysis of the relationship between neighboring myonuclei. The three dimensional representation enables extraction of a number of features for individual myonuclei, e.g., size and shape of a nucleus, and the myonuclear domain (in which each myonucleus control the gene products). This project investigates data sets from single muscle fibers sampled from mouse, rat, pig, human, horse and rhino to determine the myonuclei arrangement between species with a 100,000 fold difference in body weight.

The appropriate image analysis tools needed for gaining the understanding of organization in three dimensional volume images are developed within the project to facilitate the analysis of similarities between species, and unique features within a species. The accumulated understanding of the spatial organization of myonuclei, and the effect of individual myonuclei size, will lead to an increased knowledge of basic mechanisms underlying muscle wasting in various neuromuscular disorders. This knowledge will hopefully lead to new therapeutic strategies that can be evaluated in experimental animal models prior to clinical testing trials in patients.

National Category
Computer Vision and Robotics (Autonomous Systems)
Identifiers
urn:nbn:se:uu:diva-12567 (URN)
Note
Abstract at p. 101.Available from: 2008-01-07 Created: 2008-01-07 Last updated: 2018-08-24Bibliographically approved
Höglund, A.-S., Liu, J., Karlsson, P., Lindblad, J., Borgefors, G., Bengtsson, E. & Larsson, L. (2007). The spatial distribution of nuclei in single skeletal muscle cells as visualised by 3-D images:: the differences in organisation between species and between healthy cells and cells affected by disease. In: Biophysical Journal: 637A-637A Suppl. S.
Open this publication in new window or tab >>The spatial distribution of nuclei in single skeletal muscle cells as visualised by 3-D images:: the differences in organisation between species and between healthy cells and cells affected by disease
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2007 (English)In: Biophysical Journal: 637A-637A Suppl. S, 2007Conference paper, Published paper (Other scientific)
Identifiers
urn:nbn:se:uu:diva-12445 (URN)0006-3495 (ISBN)
Available from: 2007-12-20 Created: 2007-12-20 Last updated: 2018-08-24
Nordquist, J., Höglund, A.-S., Norman, H., Tang, X., Dworkin, B. & Larsson, L. (2007). Transcription factors in muscle atrophy caused by blocked neuromuscular transmission and muscle unloading in rats. Molecular medicine (Cambridge, Mass. Print), 13(9-10), 461-470
Open this publication in new window or tab >>Transcription factors in muscle atrophy caused by blocked neuromuscular transmission and muscle unloading in rats
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2007 (English)In: Molecular medicine (Cambridge, Mass. Print), ISSN 1076-1551, E-ISSN 1528-3658, Vol. 13, no 9-10, p. 461-470Article in journal (Refereed) Published
Abstract [en]

The muscle wasting associated with long-term intensive care unit (ICU) treatment has a negative effect on muscle function resulting in prolonged periods of rehabilitation and a decreased quality of life. To identify mechanisms behind this form of muscle wasting, we have used a rat model designed to mimic the conditions in an ICU. Rats were pharmacologically paralyzed with a postsynaptic blocker of neuromuscular transmission, and mechanically ventilated for one to two weeks, thereby unloading the limb muscles. Transcription factors were analyzed for cellular localization and nuclear concentration in the fast-twitch muscle extensor digitorum longus (EDL) and in the slow-twitch soleus. Significant muscle wasting and upregulation of mRNA for the ubiquitin ligases MAFbx and MuRF1 followed the treatment. The IκB family–member Bcl-3 displayed a concomitant decrease in concentration, suggesting altered κB controlled gene expression, although NFκB p65 was not significantly affected. The nuclear levels of the glucocorticoid receptor (GR) and the thyroid receptor α1 (TRα1) were altered and also suggested as potential mediators of the MAFbx- and MuRF1-induction in the absence of induced Foxo1. We believe that this model, and the strategy of quantifying nuclear proteins, will provide a valuable tool for further, more detailed, analyses of the muscle wasting occurring in patients kept on a mechanical ventilator.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-94834 (URN)10.2119/2006-00066.Nordquist (DOI)000250280400002 ()17622304 (PubMedID)
Available from: 2006-09-18 Created: 2006-09-18 Last updated: 2017-12-14Bibliographically approved
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