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Bergman, Kristoffer
Publications (10 of 10) Show all publications
Hulsart-Billström, G., Bergman, K., Bowden, T., Engstrand, T., Hilborn, J. & Larsson, S. (2011). The Effect of Incubation Time of Preformed Injectable Hydrogels on Bone Formation when used as Carrier for rhBMP-2. In: TERMIS-EU 2011 Abstracts: . Paper presented at TERMIS EU 2011 Annual Meeting, 7-10 June, 2011, Granada, Spain.
Open this publication in new window or tab >>The Effect of Incubation Time of Preformed Injectable Hydrogels on Bone Formation when used as Carrier for rhBMP-2
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2011 (English)In: TERMIS-EU 2011 Abstracts, 2011Conference paper, Published paper (Refereed)
National Category
Natural Sciences Polymer Chemistry
Research subject
Biology; Orthopaedics; Materials Science; Chemistry with specialization in Polymer Chemistry
Identifiers
urn:nbn:se:uu:diva-159890 (URN)
Conference
TERMIS EU 2011 Annual Meeting, 7-10 June, 2011, Granada, Spain
Available from: 2011-10-11 Created: 2011-10-11 Last updated: 2013-07-04Bibliographically approved
Docherty-Skogh, A.-C., Bergman, K., Waern, M. J., Ekman, S., Hultenby, K., Ossipov, D., . . . Engstrand, T. (2010). Bone morphogenetic protein-2 delivered by hyaluronan-based hydrogel induces massive bone formation and healing of cranial defects in minipigs. Plastic and reconstructive surgery (1963), 125(5), 1383-1392
Open this publication in new window or tab >>Bone morphogenetic protein-2 delivered by hyaluronan-based hydrogel induces massive bone formation and healing of cranial defects in minipigs
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2010 (English)In: Plastic and reconstructive surgery (1963), ISSN 0032-1052, E-ISSN 1529-4242, Vol. 125, no 5, p. 1383-1392Article in journal (Refereed) Published
Abstract [en]

Background: Reconstruction of large craniofacial bone defects is a challenge using bone transplants or alloplastic materials. The use of bone morphogenetic protein (BMP)-2 together with a suitable carrier is an attractive option that may facilitate new bone formation. The authors have developed a hydrogel that is formed in situ by the cross-linking of multifunctional hyaluronic acid and polyvinyl alcohol derivatives mixed with hydroxyapatite nanoparticles, in the presence of BMP-2. The aim of this study was to evaluate the suitability of the hydrogel as a carrier for BMP-2 in repairing critical size cranial defects in a minipig model. Methods: Cranial defects (2 × 4 cm) were created in 14 minipigs. The experimental groups were as follows: group 1, craniotomy and application of 5 ml of hydrogel with 1.25 mg of BMP-2 (n = 6); group 2, craniotomy and application of 5 ml of hydrogel without BMP-2 (n = 6); and group 3, craniotomy with no further treatment (n = 2). Results: After 3 months, computed tomographic and histologic examinations were performed. There was spontaneous ossification in the untreated group, but the healing was incomplete. The hydrogel alone demonstrated no further effects. The addition of 1.25 mg of BMP-2 to the hydrogel induced a greater than 100 percent increase in bone volume (p = 0.003) and complete healing of the defects. Histologic examination revealed compact lamellar bone in the BMP group without intertrabecular fibrous tissue, as was seen in the other groups. The hydrogel was resorbed completely within 3 months and, importantly, caused no inflammatory reaction. Conclusion: The injectable hydrogel may be favorable as a BMP-2 carrier for bone reconstruction.

National Category
Medical and Health Sciences Chemical Sciences
Identifiers
urn:nbn:se:uu:diva-139503 (URN)10.1097/PRS.0b013e3181d629dc (DOI)000276886600010 ()20440158 (PubMedID)
Available from: 2010-12-27 Created: 2010-12-27 Last updated: 2017-12-11Bibliographically approved
Hilborn, J., Ossipov, D., Bergman, K., Bowden, T., Engstrand, T. & Piskonova, S. (2008). Preparing Bone Using an Injectable Hydrogel Scaffold. In: TERMIS EU 2008 Porto Meeting June 22–26, 2008 Porto Congress Center–Alfândega Portugal: [Published in Tissue Engineering. Part A, vol. 14, nr. 5] (pp. 769-770). Mary Ann Liebert Inc., 14(5)
Open this publication in new window or tab >>Preparing Bone Using an Injectable Hydrogel Scaffold
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2008 (English)In: TERMIS EU 2008 Porto Meeting June 22–26, 2008 Porto Congress Center–Alfândega Portugal: [Published in Tissue Engineering. Part A, vol. 14, nr. 5], Mary Ann Liebert Inc. , 2008, Vol. 14, no 5, p. 769-770Conference paper, Published paper (Other academic)
Place, publisher, year, edition, pages
Mary Ann Liebert Inc., 2008
National Category
Chemical Sciences
Identifiers
urn:nbn:se:uu:diva-17726 (URN)10.1089/tea.2008.1504 (DOI)
Available from: 2008-08-19 Created: 2008-08-19 Last updated: 2011-03-17Bibliographically approved
Brännvall, K., Bergman, K., Wallenquist, U., Svahn, S., Bowden, T., Hilborn, J. & Forsberg-Nilsson, K. (2007). Enhanced neuronal differentiation in a three-dimensional collagen-hyaluronan matrix. Journal of Neuroscience Research, 85(10), 2138-2146
Open this publication in new window or tab >>Enhanced neuronal differentiation in a three-dimensional collagen-hyaluronan matrix
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2007 (English)In: Journal of Neuroscience Research, ISSN 0360-4012, E-ISSN 1097-4547, Vol. 85, no 10, p. 2138-2146Article in journal (Refereed) Published
Abstract [en]

Efficient 3D cell systems for neuronal induction are needed for future use in tissue regeneration. In this study, we have characterized the ability of neural stem/progenitor cells (NS/PC) to survive, proliferate, and differentiate in a collagen type I-hyaluronan scaffold. Embryonic, postnatal, and adult NS/PC were seeded in the present 3D scaffold and cultured in medium containing epidermal growth factor and fibroblast growth factor-2, a condition that stimulates NS/PC proliferation. Progenitor cells from the embryonic brain had the highest proliferation rate, and adult cells the lowest, indicating a difference in mitogenic responsiveness. NS/PC from postnatal stages down-regulated nestin expression more rapidly than both embryonic and adult NS/PC, indicating a faster differentiation process. After 6 days of differentiation in the 3D scaffold, NS/PC from the postnatal brain had generated up to 70% neurons, compared with 14% in 2D. NS/PC from other ages gave rise to approximately the same proportion of neurons in 3D as in 2D (9-26% depending on the source for NS/PC). In the postnatal NS/PC cultures, the majority of III-tubulin-positive cells expressed glutamate, -aminobutyric acid, and synapsin I after 11 days of differentiation, indicating differentiation to mature neurons. Here we report that postnatal NS/PC survive, proliferate, and efficiently form synapsin I-positive neurons in a biocompatible hydrogel.

Keywords
3D cultures, neural stem/progenitor cells, hydrogel, scaffold, neurogenesis
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-11683 (URN)10.1002/jnr.21358 (DOI)000248516700008 ()17520747 (PubMedID)
Available from: 2007-10-17 Created: 2007-10-17 Last updated: 2017-12-11Bibliographically approved
Bergman, K., Elvingson, C., Hilborn, J., Svensk, G. & Bowden, T. (2007). Hyaluronic acid derivatives prepared in aqueous media by triazine-activated amidation. Biomacromolecules, 8(7), 2190-2195
Open this publication in new window or tab >>Hyaluronic acid derivatives prepared in aqueous media by triazine-activated amidation
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2007 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 8, no 7, p. 2190-2195Article in journal (Refereed) Published
Abstract [en]

A method is presented for the preparation of hyaluronic acid derivatives obtained through triazine-activated amidation. A number of amines were successfully reacted with hyaluronic acid carboxyl groups using 2-chloro-4,6-dimethoxy-1,3,5-triazine as an activating species in a mixture of water and acetonitrile under neutral conditions. By varying the amount of triazine reagent, it was possible to control the degree of modification. Depending on the amine chosen, degrees of modification ranging from 3 to 20% were obtained when using 0.5 equiv of the triazine to hyaluronic acid carboxyl groups. The possibility to perform the reaction in a mixture of water and acetonitrile facilitates the introduction of a wide range of both hydrophilic and hydrophobic amines. Triazine-activated amidation appears to be a highly versatile, controllable, and relatively mild technique for modification of hyaluronic acid, and we predict that it will be useful in the design of novel hyaluronic acid based biomaterials.

Keywords
Oside polymer, Experimental study, Mild operating conditions, Aqueous medium, Triazine derivatives, Activation, Primary amine, Amidation, Chemical modification, Preparation, Modified material, Hyaluronic acid
National Category
Chemical Sciences
Identifiers
urn:nbn:se:uu:diva-97729 (URN)10.1021/bm0701604 (DOI)000247820000022 ()17579475 (PubMedID)
Available from: 2008-11-14 Created: 2008-11-14 Last updated: 2017-12-14Bibliographically approved
Hilborn, J., Bergman, K., Ossipov, D., Bowden, T. & Engstrand, T. (2007). Preparation and evaluation of an injectable hyaluronan hydrogel for therapeutic applications. In: TERMIS-EU Meeting Abstracts, London, UK, September 4–7, 2007: [published in Tissue Engineering, vol. 13, nr. 7] (pp. 1747-1747). Mary Ann Liebert Inc., 13(7)
Open this publication in new window or tab >>Preparation and evaluation of an injectable hyaluronan hydrogel for therapeutic applications
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2007 (English)In: TERMIS-EU Meeting Abstracts, London, UK, September 4–7, 2007: [published in Tissue Engineering, vol. 13, nr. 7], Mary Ann Liebert Inc. , 2007, Vol. 13, no 7, p. 1747-1747Conference paper, Published paper (Other academic)
Place, publisher, year, edition, pages
Mary Ann Liebert Inc., 2007
National Category
Chemical Sciences
Identifiers
urn:nbn:se:uu:diva-16549 (URN)10.1089/ten.2007.1501 (DOI)
Available from: 2008-08-19 Created: 2008-08-19 Last updated: 2011-03-17Bibliographically approved
Bergman, K., Hilborn, J. & Bowden, T. (2007). Preparation and evaluation of an injectable hyaluronan hydrogel for therapeutic applications. In: 7th International conference on Hyaluronan, 22nd-27th April 2007, Charleston, South Carolina, USA.
Open this publication in new window or tab >>Preparation and evaluation of an injectable hyaluronan hydrogel for therapeutic applications
2007 (English)In: 7th International conference on Hyaluronan, 22nd-27th April 2007, Charleston, South Carolina, USA, 2007Conference paper, Published paper (Other (popular science, discussion, etc.))
National Category
Inorganic Chemistry
Identifiers
urn:nbn:se:uu:diva-16690 (URN)
Available from: 2008-06-02 Created: 2008-06-02 Last updated: 2009-08-21Bibliographically approved
Bergman, K., Hilborn, J. & Bowden, T. (2006). Modification of Hyaluronan by Triazine-Promoted Amidation in Aqueous Media. In: 232nd ACS National Meeting, 10th-14th Sept. 2006, San Francisco, California, USA.
Open this publication in new window or tab >>Modification of Hyaluronan by Triazine-Promoted Amidation in Aqueous Media
2006 (English)In: 232nd ACS National Meeting, 10th-14th Sept. 2006, San Francisco, California, USA, 2006Conference paper, Published paper (Other (popular science, discussion, etc.))
National Category
Inorganic Chemistry
Identifiers
urn:nbn:se:uu:diva-17473 (URN)
Available from: 2008-06-24 Created: 2008-06-24 Last updated: 2009-08-06Bibliographically approved
Bergman, K., Bowden, T. & Hilborn, J. (2005). Hyaluronic acid cross-linking chemistry. In: 8th International Symposium of Polymers for Advanced Technologies, 13th-16th Sept. 2005, Budapest, Hungary.
Open this publication in new window or tab >>Hyaluronic acid cross-linking chemistry
2005 (English)In: 8th International Symposium of Polymers for Advanced Technologies, 13th-16th Sept. 2005, Budapest, Hungary, 2005Conference paper, Published paper (Other scientific)
National Category
Inorganic Chemistry
Identifiers
urn:nbn:se:uu:diva-17488 (URN)
Available from: 2008-06-25 Created: 2008-06-25
Bergman, K. & Hilborn, J. (2003). Mild modifications of hyaluronan for fine-tuning of mechanical and chemical properties. In: Europolymer Congress, 2003, Stockholm, Sweden.
Open this publication in new window or tab >>Mild modifications of hyaluronan for fine-tuning of mechanical and chemical properties
2003 (English)In: Europolymer Congress, 2003, Stockholm, Sweden, 2003Conference paper, Published paper (Other academic)
National Category
Inorganic Chemistry
Identifiers
urn:nbn:se:uu:diva-17489 (URN)
Available from: 2008-06-25 Created: 2008-06-25 Last updated: 2009-08-06Bibliographically approved
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