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Zardán Gómez de la Torre, Teresa
Alternative names
Publications (10 of 55) Show all publications
Sepehri, S., Agnarsson, B., Zardán Gómez de la Torre, T., Schneiderman, J. F., Blomgren, J., Jesorka, A., . . . Kalaboukhov, A. (2019). Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay. Biosensors, 9(3), Article ID 109.
Open this publication in new window or tab >>Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay
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2019 (English)In: Biosensors, ISSN 2079-6374, Vol. 9, no 3, article id 109Article in journal (Refereed) Published
Abstract [en]

The specific binding of oligonucleotide-tagged 100 nm magnetic nanoparticles (MNPs) to rolling circle products (RCPs) is investigated using our newly developed differential homogenous magnetic assay (DHMA). The DHMA measures ac magnetic susceptibility from a test and a control samples simultaneously and eliminates magnetic background signal. Therefore, the DHMA can reveal details of binding kinetics of magnetic nanoparticles at very low concentrations of RCPs. From the analysis of the imaginary part of the DHMA signal, we find that smaller MNPs in the particle ensemble bind first to the RCPs. When the RCP concentration increases, we observe the formation of agglomerates, which leads to lower number of MNPs per RCP at higher concentrations of RCPs. The results thus indicate that a full frequency range of ac susceptibility observation is necessary to detect low concentrations of target RCPs and a long amplification time is not required as it does not significantly increase the number of MNPs per RCP. The findings are critical for understanding the underlying microscopic binding process for improving the assay performance. They furthermore suggest DHMA is a powerful technique for dynamically characterizing the binding interactions between MNPs and biomolecules in fluid volumes.

Keywords
magnetic nanoparticle, bioassay, differential homogenous magnetic assay, immobilization, binding kinetics, rolling circle amplification product
National Category
Nano Technology Pharmacology and Toxicology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-393235 (URN)10.3390/bios9030109 (DOI)000487949000022 ()31533330 (PubMedID)
Funder
Swedish Research Council, 2015-03640Swedish Foundation for Strategic Research , SBE13-0125Knut and Alice Wallenberg Foundation
Available from: 2019-09-17 Created: 2019-09-17 Last updated: 2019-10-30Bibliographically approved
Vall, M., Ferraz, N., Cheung, O., Strömme, M. & Zardán Gómez de la Torre, T. (2019). Exploring the use of amine modified mesoporous magnesium carbonate for the delivery of salicylic acid in topical formulations: : in vitro cytotoxicity and drug release studies. Molecules, 24(9), Article ID 1820.
Open this publication in new window or tab >>Exploring the use of amine modified mesoporous magnesium carbonate for the delivery of salicylic acid in topical formulations: : in vitro cytotoxicity and drug release studies
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2019 (English)In: Molecules, ISSN 1420-3049, E-ISSN 1420-3049, Vol. 24, no 9, article id 1820Article in journal (Refereed) Published
Place, publisher, year, edition, pages
MDPI, 2019
Keywords
mesoporous; magnesium carbonate; amine functionalization; cytotoxicity; salicylic acid; drug release
National Category
Nano Technology
Identifiers
urn:nbn:se:uu:diva-381421 (URN)10.3390/molecules24091820 (DOI)
Available from: 2019-04-10 Created: 2019-04-10 Last updated: 2020-02-14Bibliographically approved
Sepehri, S., Kalaboukhov, A., Zardán Gómez de la Torre, T., Schneiderman, J. F., Jesorka, A., Nilsson, M., . . . Winkler, D. (2019). Fast, Ultrasensitive Differential Magnetic DNA assay Using HTS SQUID Gradiometer . In: 14th European Conference on Applied Superconductivity: . Paper presented at 14th European Conference on Applied Superconductivity, EUCAS2019. 1-5 September, 2019, Glasgow, UK. Glasgow
Open this publication in new window or tab >>Fast, Ultrasensitive Differential Magnetic DNA assay Using HTS SQUID Gradiometer
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2019 (English)In: 14th European Conference on Applied Superconductivity, Glasgow, 2019Conference paper, Oral presentation with published abstract (Refereed)
Place, publisher, year, edition, pages
Glasgow: , 2019
National Category
Other Materials Engineering
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-395334 (URN)
Conference
14th European Conference on Applied Superconductivity, EUCAS2019. 1-5 September, 2019, Glasgow, UK
Available from: 2019-10-17 Created: 2019-10-17 Last updated: 2020-04-29Bibliographically approved
Sepehri, S., Zardán Gómez de la Torre, T., Schneiderman, J. F., Blomgren, J., Jesorka, A., Johansson, C., . . . Kalaboukhovf, A. (2019). Homogeneous Differential Magnetic Assay. ACS SENSORS, 4(9), 2381-2388
Open this publication in new window or tab >>Homogeneous Differential Magnetic Assay
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2019 (English)In: ACS SENSORS, ISSN 2379-3694, Vol. 4, no 9, p. 2381-2388Article in journal (Refereed) Published
Abstract [en]

Assays are widely used for detection of various targets, including pathogens, drugs, and toxins. Homogeneous assays are promising for the realization of point-of-care diagnostics as they do not require separation, immobilization, or washing steps. For low concentrations of target molecules, the speed and sensitivity of homogeneous assays have hitherto been limited by slow binding kinetics, time-consuming amplification steps, and the presence of a high background signal. Here, we present a homogeneous differential magnetic assay that utilizes a differential magnetic readout that eliminates previous limitations of homogeneous assays. The assay uses a gradiometer sensor configuration combined with precise microfluidic sample handling. This enables simultaneous differential measurement sample containing a synthesized Vibrio cholerae target and a negative control sample, which reduces the background signal and increases the readout speed. Very low concentrations of targets down to femtomolar levels are thus detectable without any additional amplification of the number of targets. Our homogeneous differential magnetic assay method opens new possibilities for rapid and highly sensitive diagnostics at the point of care.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2019
Keywords
homogeneous differential magnetic assay, magnetic nanoparticle, volumetric detection, rolling circle amplification, Brownian relaxation, binding kinetics
National Category
Biomedical Laboratory Science/Technology
Identifiers
urn:nbn:se:uu:diva-395849 (URN)10.1021/acssensors.9b00969 (DOI)000488424100021 ()31397152 (PubMedID)
Funder
Swedish Foundation for Strategic Research , SBE13-0125Swedish Research Council, 2015-03640Knut and Alice Wallenberg Foundation
Available from: 2019-10-25 Created: 2019-10-25 Last updated: 2019-10-25Bibliographically approved
Sepehri, S., Zardán Gómez de la Torre, T., Schneiderman, J. F., Blomgren, J., Jesorka, A., Johansson, C., . . . Kalaboukhov, A. (2019). Study of magnetic beads-DNA coils binding kinetics using a differential homogeneous magnetic assay. In: The European Magnetism Association (Ed.), Joint European Magnetic Symposia (JEMS) 2019: . Paper presented at Joint European Magnetic Symposia (JEMS), Uppsala, August 26-30 2019.. Uppsala
Open this publication in new window or tab >>Study of magnetic beads-DNA coils binding kinetics using a differential homogeneous magnetic assay
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2019 (English)In: Joint European Magnetic Symposia (JEMS) 2019 / [ed] The European Magnetism Association, Uppsala, 2019Conference paper, Oral presentation with published abstract (Refereed)
Abstract [en]

The binding kinetics of magnetic nanoparticles (MNPs) to rolling circle amplification products (RCPs) is investigated using a differential homogenous magnetic assay (DHMA)1. The DHMA utilizes a microfluidic device to measure the differential ac susceptibility signal between a reference and a test sample, taking advantage of the symmetry in a high-Tc SQUID gradiometer sensor2. The DHMA signal is related to the relative differences in the particle distribution of the two samples, and the background magnetic signal is thus eliminated. Therefore, minuscule changes in the nanoparticle’s concentration and size distribution of the test sample are directly detectable in the solution. This makes the DHMA a superior technique to characterize the binding interaction of the MNPs to biomolecules like RCPs specially at very low concentrations. The DHMA reveals that there is a competitive dynamic process between the MNP labelled RCPs and the unbound MNPs in the solution as a function of the RCP concentrations. The evidence of this dynamic in the signal fades as the MNP-RCP agglomerates are formed. The DHMA also shows that the smaller MNPs in the MNP size distribution take precedence over the larger MNP in immobilization on the RCPs. Comparing the DHMA responses with the turn-off detection method indicates that a full frequency range ac susceptibility observation is necessary when detecting low concentration of target RCPs. The findings are critical for understanding the underlying microscopic binding process and improving the assay performance. [1] Sepehri, S. et al, Differential homogeneous magnetic assay. Submitted. [2] Sepehri, S. et al. Volume-amplified magnetic bioassay integrated with microfluidic sample handling and high-Tc SQUID magnetic readout. APL Bioeng. 2, 016102 (2018).

Place, publisher, year, edition, pages
Uppsala: , 2019
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-389857 (URN)
Conference
Joint European Magnetic Symposia (JEMS), Uppsala, August 26-30 2019.
Available from: 2019-07-30 Created: 2019-07-30 Last updated: 2019-07-30
Ahrentorp, F., Blomgren, J., Jonasson, C., Sepehri, S., kalabukhov, A., Jesorka, A., . . . Johansson, C. (2018). Development of a sensitive induction based magnetic nanoparticle biodetection method. In: 12th International Conference on the Scientific and Clinical Applications of Magnetic Carriers: . Paper presented at 12th International Conference on the Scientific and Clinical Applications of Magnetic Carriers May 22 - 26, 2018 | Copenhagen, Denmark. , Article ID Poster 250.
Open this publication in new window or tab >>Development of a sensitive induction based magnetic nanoparticle biodetection method
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2018 (English)In: 12th International Conference on the Scientific and Clinical Applications of Magnetic Carriers, 2018, article id Poster 250Conference paper, Poster (with or without abstract) (Other academic)
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-364871 (URN)
Conference
12th International Conference on the Scientific and Clinical Applications of Magnetic Carriers May 22 - 26, 2018 | Copenhagen, Denmark
Available from: 2018-11-05 Created: 2018-11-05 Last updated: 2020-05-18Bibliographically approved
Blomgren, J., Ahrentorp, F., Ilver, D., Jonasson, C., Sepehri, S., Kalaboukhov, A., . . . Johansson, C. (2018). Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method.. Nanomaterials (Basel, Switzerland), 8(11), Article ID E887.
Open this publication in new window or tab >>Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method.
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2018 (English)In: Nanomaterials (Basel, Switzerland), E-ISSN 2079-4991, Vol. 8, no 11, article id E887Article in journal (Refereed) Published
Abstract [en]

We developed a novel biodetection method for influenza virus based on AC magnetic susceptibility measurement techniques (the DynoMag induction technique) together with functionalized multi-core magnetic nanoparticles. The sample consisting of an incubated mixture of magnetic nanoparticles and rolling circle amplified DNA coils is injected into a tube by a peristaltic pump. The sample is moved as a plug to the two well-balanced detection coils and the dynamic magnetic moment in each position is read over a range of excitation frequencies. The time for making a complete frequency sweep over the relaxation peak is about 5 minutes (10 Hz⁻10 kHz with 20 data points). The obtained standard deviation of the magnetic signal at the relaxation frequency (around 100 Hz) is equal to about 10-5 (volume susceptibility SI units), which is in the same range obtained with the DynoMag system. The limit of detection with this method is found to be in the range of 1 pM.

Keywords
AC susceptibility, Brownian relaxation, magnetic biosensing, magnetic nanoparticles, multi-core particles
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-366859 (URN)10.3390/nano8110887 (DOI)000451316100022 ()30388776 (PubMedID)
Funder
Swedish Foundation for Strategic Research
Available from: 2018-11-26 Created: 2018-11-26 Last updated: 2019-01-24Bibliographically approved
Tian, B., Qiu, Z., Ma, J., Zardán Gómez de la Torre, T., Donolato, M., Fougt Hansen, M., . . . Strömberg, M. (2018). Optomagnetic detection of microRNA based on duplex-specific nuclease assisted targetrecycling and core-satellite magnetic superstructures. In: : . Paper presented at Biosensors 2018 conference. , Article ID Abstract No. 0242.
Open this publication in new window or tab >>Optomagnetic detection of microRNA based on duplex-specific nuclease assisted targetrecycling and core-satellite magnetic superstructures
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2018 (English)Conference paper, Poster (with or without abstract) (Refereed)
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Solid State Physics
Identifiers
urn:nbn:se:uu:diva-363320 (URN)
Conference
Biosensors 2018 conference
Funder
Swedish Research Council Formas, 221-2012-444Swedish Research Council Formas, 2011-1692EU, FP7, Seventh Framework Programme, 604448-NanoMag
Available from: 2018-10-16 Created: 2018-10-16 Last updated: 2019-04-24
Sepehri, S., Kalaboukhov, A., Schneiderman, J., Zardán Gómez de la Torre, T., Jesorka, A., Strömme, M. & Winkler, D. (2018). Ultra-Sensitive Magnetic Bioassay Using a High-TC SQUID Gradiometer. In: International Workshop for Superconducting Sensors and Detectors 2018: . Paper presented at International Workshop for Superconducting Sensors and Detectors 2018, July 24-27 (2018), Sydney, Australia.
Open this publication in new window or tab >>Ultra-Sensitive Magnetic Bioassay Using a High-TC SQUID Gradiometer
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2018 (English)In: International Workshop for Superconducting Sensors and Detectors 2018, 2018Conference paper, Published paper (Refereed)
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-366857 (URN)
Conference
International Workshop for Superconducting Sensors and Detectors 2018, July 24-27 (2018), Sydney, Australia
Available from: 2018-11-26 Created: 2018-11-26 Last updated: 2019-03-14Bibliographically approved
Sepehri, S., Eriksson, E., Kalaboukhov, A., Zardán Gómez de la Torre, T., Kustanovich, K., Jesorka, A., . . . Winkler, D. (2018). Volume-amplified magnetic bioassay integrated with microfluidic sample handling and high-Tc SQUID magnetic readout. APL Bioengineering, 2(1), Article ID 016102.
Open this publication in new window or tab >>Volume-amplified magnetic bioassay integrated with microfluidic sample handling and high-Tc SQUID magnetic readout
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2018 (English)In: APL Bioengineering, Vol. 2, no 1, article id 016102Article in journal (Refereed) Published
Abstract [en]

A bioassay based on a high-Tc superconducting quantum interference device (SQUID) reading out functionalized magnetic nanoparticles (fMNPs) in a prototype microfluidic platform is presented. The target molecule recognition is based on volume amplification using padlock-probe-ligation followed by rolling circle amplification (RCA). The MNPs are functionalized with single-stranded oligonucleotides, which give a specific binding of the MNPs to the large RCA coil product, resulting in a large change in the amplitude of the imaginary part of the ac magnetic susceptibility. The RCA products from amplification of synthetic Vibrio cholera target DNA were investigated using our SQUID ac susceptibility system in microfluidic channel with an equivalent sample volume of 3 μl. From extrapolation of the linear dependence of the SQUID signal versus concentration of the RCA coils, it is found that the projected limit of detection for our system is about 1.0 × 105 RCA coils (0.2 × 10−18 mol), which is equivalent to 66 fM in the 3 μl sample volume. This ultra-high magnetic sensitivity and integration with microfluidic sample handling are critical steps towards magnetic bioassays for rapid detection of DNA and RNA targets at the point of care.

Place, publisher, year, edition, pages
American Institute of Physics, 2018
National Category
Other Materials Engineering
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-349146 (URN)10.1063/1.4999713 (DOI)000455056800003 ()
Funder
Swedish Foundation for Strategic Research , SBE13-0125Knut and Alice Wallenberg Foundation
Available from: 2018-04-22 Created: 2018-04-22 Last updated: 2019-01-23Bibliographically approved
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