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Saldeen, Tom
Publications (10 of 50) Show all publications
Ahn, C. M., Sandler, H. & Saldeen, T. (2012). Decreased lung hyaluronan in a model of ARDS in the rat: Effect of an inhibitor of leukocyte elastase. Upsala Journal of Medical Sciences, 117(1), 1-9
Open this publication in new window or tab >>Decreased lung hyaluronan in a model of ARDS in the rat: Effect of an inhibitor of leukocyte elastase
2012 (English)In: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 117, no 1, p. 1-9Article in journal (Refereed) Published
Abstract [en]

Background. Hyaluronan (HA) is a component of the extracellular matrix in lung tissue and is normally present at low concentrations in blood. HA is rapidly cleared from blood by the liver. Increased concentrations of plasma HA have been found in patients with acute respiratory distress syndrome (ARDS). We investigated changes in HA levels in plasma, bronchoalveolar lavage fluid (BALF), and lung, and their relationship to pretreatment with a leukocyte elastase inhibitor in a rat model of ARDS.

Methods. Rats were randomly assigned to three groups: control, thrombin, and thrombin plus elastase inhibitor. By use of a radiometric assay, HA was measured in lungs, BALF, and plasma. Tissue samples from the lungs were stained for HA and examined microscopically. Liver circulation and cardiac output were monitored using radiolabeled microspheres.

Results. Infusion of thrombin produced a pronounced increase in wet weight to dry weight ratio, and relative lung water content. This increase was blunted by a leukocyte elastase inhibitor. A decrease in lung HA and increases in both BALF and plasma HA were found. The leukocyte elastase inhibitor counteracted not only the decrease in lung tissue HA, but also the increase in plasma HA. Histologically, there was decreased HA-staining of peribronchial and perivascular areas in the injured rat lung. Decreased liver perfusion was observed after infusion of thrombin.

Conclusions. The decrease in lung HA may be involved in the development of pulmonary edema in this ARDS model, and leukocyte elastase may be one cause of this decrease. In addition, an elevated plasma HA level may be an indicator of lung injury.

Keywords
Acute lung injury, acute respiratory distress syndrome, hyaluronan, leukocyte elastase inhibitor, pulmonary edema
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-170332 (URN)10.3109/03009734.2011.622812 (DOI)000300304000001 ()
Available from: 2012-03-13 Created: 2012-03-12 Last updated: 2017-12-07Bibliographically approved
Engström, K., Saldeen, A.-S., Yang, B., Mehta, J. L. & Saldeen, T. (2009). Effect of fish oils containing different amounts of EPA, DHA, and antioxidants on plasma and brain fatty acids and brain nitric oxide synthase activity in rats. Upsala Journal of Medical Sciences, 114(4), 206-213
Open this publication in new window or tab >>Effect of fish oils containing different amounts of EPA, DHA, and antioxidants on plasma and brain fatty acids and brain nitric oxide synthase activity in rats
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2009 (English)In: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 114, no 4, p. 206-213Article in journal (Refereed) Published
Abstract [en]

Background. The interest in n-3 polyunsaturated fatty acids (PUFAs) has expanded significantly in the last few years, due to their many positive effects described. Consequently, the interest in fish oil supplementation has also increased, and many different types of fish oil supplements can be found on the market. Also, it is well known that these types of fatty acids are very easily oxidized, and that stability among supplements varies greatly. Aims of the study. In this pilot study we investigated the effects of two different types of natural fish oils containing different amounts of the n-3 PUFAs eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and antioxidants on plasma and brain fatty acids, blood lipids, vitamin E, and in vivo lipid peroxidation, as well as brain nitric oxide synthase (NOS) activity, an enzyme which has been shown to be important for memory and learning ability. Methods. Sprague-Dawley rats were divided into four groups and fed regular rat chow pellets enriched with 5% (w/w) of butter (control group), a natural fish oil (17.4% EPAand 11.7% DHA, referred to as EPA-rich), and a natural fish oil rich in DHA (7.7% EPA and 28.0% DRA, referred to as DHA-rich). Both of the fish oils were stabilized by a commercial antioxidant protection system (Pufanox (R)) at production. The fourth group received the same DHA-rich oil, but without Pufanox (R) stabilization (referred to as unstable). As an index of stability of the oils, their peroxide values were repeatedly measured during 9 weeks. The dietary treatments continued until sacrifice, after 10 days. Results. Stability of the oils varied greatly. It took the two stabilized oils 9 weeks to reach the same peroxide value as the unstable oil reached after only a few days. Both the stabilized EPA- and DHA-rich diets lowered the triacylglycerols and total cholesterol compared to control (45%, P < 0.05 and -54%, P < 0.001; -31%, P< 0.05 and -25%, P< 0.01) and so did the unstable oil, but less efficiently. Only the unstable oil increased in vivo lipid peroxidation significantly compared to control (+40%, P < 0.001). Most of the fatty acids in the plasma phospholipids were significantly affected by both the EPA- and DHA-rich diets compared to control, reflecting their specific fatty acid pattern. The unstable oil diet resulted in smaller changes, especially in n-3 PUFAs. In the brain phospholipids the changes were less pronounced, and only the diet enriched with the stabilized DHA-rich oil resulted in a significantly greater incorporation of DHA (+ 13%, P < 0.01), as well as total n-3 PUFAs (+ 13%, P < 0.01) compared to control. Only the stabilized DHA-rich oil increased the brain NOS activity (+33%, P < 0.01). Conclusions. Both the EPA- and DHA-rich diets affected the blood lipids in a similarly positive manner, and they both had a large impact on plasma phospholipid fatty acids. It was only the unstable oil that increased in vivo lipid peroxidation. However, the intake of DHA was more important than that of EPA for brain phospholipid DHA enrichment and brain NOS activity, and the stability of the fish oil was also important for these effects.

Keywords
Antioxidants, brain, DHA, EPA, fish oil, lipid peroxidation, nitric oxide synthase
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-137845 (URN)10.3109/03009730903268958 (DOI)000273527100002 ()
Available from: 2010-12-17 Created: 2010-12-16 Last updated: 2017-12-11Bibliographically approved
Saldeen, T. (2008). Allt om Omega-3 - Vitaminet från havet: Hur balanserad omegadiet ger dig bättre hälsa och viktkontroll (2ed.). Uppsala: Swedehealth Press
Open this publication in new window or tab >>Allt om Omega-3 - Vitaminet från havet: Hur balanserad omegadiet ger dig bättre hälsa och viktkontroll
2008 (Swedish)Book (Other academic)
Place, publisher, year, edition, pages
Uppsala: Swedehealth Press, 2008. p. 189 Edition: 2
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-101012 (URN)978-91-975950-2-5 (ISBN)
Available from: 2009-04-16 Created: 2009-04-16 Last updated: 2015-06-11Bibliographically approved
Segger, D., Matthies, A. & Saldeen, T. (2008). Supplementation with Eskimo (R) Skin Care improves skin elasticity in women. A pilot study. Journal of dermatological treatment (Print), 19(5), 279-283
Open this publication in new window or tab >>Supplementation with Eskimo (R) Skin Care improves skin elasticity in women. A pilot study
2008 (English)In: Journal of dermatological treatment (Print), ISSN 0954-6634, E-ISSN 1471-1753, Vol. 19, no 5, p. 279-283Article in journal (Refereed) Published
Abstract [en]

Objective: To investigate the question of whether supplementation with an oral oil formulation rich in natural stable fish oil can alter skin elasticity, transepidermal water loss (TEWL), and skin roughness in healthy women. Methods: Twenty-four healthy women aged 40-60 years participated in a single-blind randomized trial for testing the effect of a proprietary oral supplement for skin nutrition (Eskimo Skin Care) on skin elasticity, TEWL, and skin roughness. Skin elasticity was measured by an optical cutometer, TEWL by a water-loss module based upon the vapour gradient principle, and skin roughness with a three-dimensional microtopography imaging system. Results: Skin elasticity increased by 10% after 3 months of treatment with the supplement, a statistically significant increase in comparison with the control group (p=0.0298). There was a trend, though not statistically significant, towards a positive influence on the skin's barrier function. No effect on the skin roughness was observed. Conclusion: Eskimo Skin Care, an oral preparation rich in natural stable fish oil, can improve skin elasticity.

Keywords
fish oil, gamma-linolenic acid, omega-3 fatty acids, skin elasticity, skin nutrition
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-109095 (URN)10.1080/09546630801958238 (DOI)000258899100005 ()
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2017-12-13Bibliographically approved
Jabbar, R. & Saldeen, T. (2006). A new predictor of risk for sudden cardiac death. Upsala Journal of Medical Sciences, 111(2), 169-177
Open this publication in new window or tab >>A new predictor of risk for sudden cardiac death
2006 (English)In: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 111, no 2, p. 169-177Article in journal (Refereed) Published
Abstract [en]

Background. Long-chain fatty acids, particularly omega-3 fatty acids found primarily in fish oil, are beneficial in different physiological conditions in the human body. High intake of omega-3 fatty acids has been found to have a strong inverse relationship to sudden cardiac death. An index showing the relationship between different fatty acids in the blood could be an important risk indicator for sudden cardiac death. Methods. Whole-blood samples from the fingertip were collected and fatty acids were measured by a new simple method using direct transmethylation. Two groups were compared: subjects who had been taking fish oil daily during the last 6 years, and subjects who had not been taking fish oil. Results. Six different fatty acid indices were calculated. Five of them take both DHA (docosahexaenoic acid) and EPA (eicosapentaenoic acid) into consideration, and the sixth includes only EPA and not DHA in the calculation. This latter index, the ratio between EPA and arachidonic acid, gave the best result, with the largest difference between the two groups. This index varied between 5 and 118 among the different individuals; 70% of the fish oil consumers having an index >= 50. Based on the present knowledge about the relationship between blood levels of omega-3 fatty acids and sudden cardiac death obtained from studies of 14,000 individuals, a subject with an index below 50 should be advised to increase his/her intake of omega-3 fatty acids. Conclusion. The EPA/arachidonic acid index may be an important new predictor of risk for sudden cardiac death, and reflects the individual requirement for consumption of omega-3 fatty acids.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-155795 (URN)000239322000001 ()16961172 (PubMedID)
Available from: 2011-06-30 Created: 2011-06-30 Last updated: 2017-12-11Bibliographically approved
Jonasson, B. & Saldeen, T. (2002). Citalopram in fatal poisoning cases.. Forensic Sci Int, 126, 1
Open this publication in new window or tab >>Citalopram in fatal poisoning cases.
2002 (English)In: Forensic Sci Int, Vol. 126, p. 1-Article in journal (Refereed) Published
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-62897 (URN)
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2015-06-11
Chen, H., Li, D., Saldeen, T., Romeo, F. & Mehta, J. (2002). Mixed tocopherol preparation is superior to alpha-tocopherol alone againsthypoxia-reoxygenation injury.. Biochem Biophys Res Commun, 291, 349
Open this publication in new window or tab >>Mixed tocopherol preparation is superior to alpha-tocopherol alone againsthypoxia-reoxygenation injury.
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2002 (English)In: Biochem Biophys Res Commun, Vol. 291, p. 349-Article in journal (Refereed) Published
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-62898 (URN)
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2015-06-11
Liu, M., Wallin, R., Wallmon, A. & Saldeen, T. (2002). Mixed tocopherols have a stronger inhibitory effect on lipid peroxidationthan alpha-tocopherol alone.. J Cardiovasc Pharmacol, 39, 714
Open this publication in new window or tab >>Mixed tocopherols have a stronger inhibitory effect on lipid peroxidationthan alpha-tocopherol alone.
2002 (English)In: J Cardiovasc Pharmacol, Vol. 39, p. 714-Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-62896 (URN)
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2015-06-11
Li, D., Chen, H., Romeo, F., Sawamura, T., Saldeen, T. & Mehta, J. (2002). Statins modulate oxidized low-density lipoprotein-mediated adhesionmolecule expression in human coronary artery endothelial cells: role ofLOX-1.. J Pharmacol Exp Ther, 302, 601
Open this publication in new window or tab >>Statins modulate oxidized low-density lipoprotein-mediated adhesionmolecule expression in human coronary artery endothelial cells: role ofLOX-1.
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2002 (English)In: J Pharmacol Exp Ther, Vol. 302, p. 601-Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-62895 (URN)
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2015-06-11
Li, D., Saldeen, T., Romeo, F. & Mehta, J. (2001). Different isoforms of tocopherols enhance nitric oxide synthasephosphorylation and inhibit human platelet aggregation and lipidperoxidation: implications in therapy with vitamin E.. J Cardiovasc Pharmacol Ther, 6, 155
Open this publication in new window or tab >>Different isoforms of tocopherols enhance nitric oxide synthasephosphorylation and inhibit human platelet aggregation and lipidperoxidation: implications in therapy with vitamin E.
2001 (English)In: J Cardiovasc Pharmacol Ther, Vol. 6, p. 155-Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-62900 (URN)
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2015-06-11
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