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Andersson, Robin
Publications (7 of 7) Show all publications
Andersson, R. (2010). Decoding the Structural Layer of Transcriptional Regulation: Computational Analyses of Chromatin and Chromosomal Aberrations. (Doctoral dissertation). Uppsala: Acta Universitatis Upsaliensis
Open this publication in new window or tab >>Decoding the Structural Layer of Transcriptional Regulation: Computational Analyses of Chromatin and Chromosomal Aberrations
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Gene activity is regulated at two separate layers. Through structural and chemical properties of DNA – the primary layer of encoding – local signatures may enable, or disable, the binding of proteins or complexes of them with regulatory potential to the DNA. At a higher level – the structural layer of encoding – gene activity is regulated through the properties of higher order DNA structure, chromatin, and chromosome organization. Cells with abnormal chromosome compaction or organization, e.g. cancer cells, may thus have perturbed regulatory activities resulting in abnormal gene activity.

Hence, there is a great need to decode the transcriptional regulation encoded in both layers to further our understanding of the factors that control activity and life of a cell and, ultimately, an organism. Modern genome-wide studies with those aims rely on data-intense experiments requiring sophisticated computational and statistical methods for data handling and analyses. This thesis describes recent advances of analyzing experimental data from quantitative biological studies to decipher the structural layer of encoding in human cells.

Adopting an integrative approach when possible, combining multiple sources of data, allowed us to study the influences of chromatin (Papers I and II) and chromosomal aberrations (Paper IV) on transcription. Combining chromatin data with chromosomal aberration data allowed us to identify putative driver oncogenes and tumor-suppressor genes in cancer (Paper IV).

Bayesian approaches enabling the incorporation of background information in the models and the adaptability of such models to data have been very useful. Their usages yielded accurate and narrow detection of chromosomal breakpoints in cancer (Papers III and IV) and reliable positioning of nucleosomes and their dynamics during transcriptional regulation at functionally relevant regulatory elements (Paper II).

Using massively parallel sequencing data, we explored the chromatin landscapes of human cells (Papers I and II) and concluded that there is a preferential and evolutionary conserved positioning at internal exons nearly unaffected by the transcriptional level. We also observed a strong association between certain histone modifications and the inclusion or exclusion of an exon in the mature gene transcript, suggesting a functional role in splicing.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2010. p. 76
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 768
Keywords
Chromatin, Nucleosome positioning, Histone modifications, Chromosomal aberrations, Transcriptional regulation, Array-CGH, Next generation sequencing, ChIP-chip
National Category
Bioinformatics and Systems Biology
Research subject
Bioinformatics
Identifiers
urn:nbn:se:uu:diva-130999 (URN)978-91-554-7897-1 (ISBN)
Public defence
2010-11-02, C4:305, BMC, Husargatan 3, Uppsala, 09:00 (English)
Opponent
Supervisors
Available from: 2010-10-12 Created: 2010-09-20 Last updated: 2010-11-11Bibliographically approved
Björkholm, P., Daniluk, P., Kryshtafovych, A., Fidelis, K., Andersson, R. & Hvidsten, T. R. (2009). Using multi-data hidden Markov models trained on local neighborhoods of protein structure to predict residue-residue contacts. Bioinformatics, 25(10), 1264-1270
Open this publication in new window or tab >>Using multi-data hidden Markov models trained on local neighborhoods of protein structure to predict residue-residue contacts
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2009 (English)In: Bioinformatics, ISSN 1367-4803, E-ISSN 1367-4811, Vol. 25, no 10, p. 1264-1270Article in journal (Refereed) Published
Abstract [en]

MOTIVATION: Correct prediction of residue-residue contacts in proteins that lack good templates with known structure would take ab initio protein structure prediction a large step forward. The lack of correct contacts, and in particular long-range contacts, is considered the main reason why these methods often fail. RESULTS: We propose a novel hidden Markov model based method for predicting residue-residue contacts from protein sequences using as training data homologous sequences, predicted secondary struc-ture and a library of local neighborhoods (local descriptors of protein structure). The library consists of recurring structural entities in-corporating short-, medium- and long-range interactions and is general enough to reassemble the cores of nearly all proteins in the PDB. The method is tested on an external test set of 606 domains with no significant sequence similarity to the training set as well as 151 domains with SCOP folds not present in the training set. Considering the top 0.2 L predictions (L = sequence length), our hidden Markov models obtained an accuracy of 22.8% for long-range interactions in new fold targets, and an average accuracy of 28.6% for long-, medium- and short-range contacts. This is a significant performance increase over currently available methods when comparing against results published in the literature.

Place, publisher, year, edition, pages
Oxford University Press, 2009
National Category
Biological Sciences
Identifiers
urn:nbn:se:uu:diva-101152 (URN)10.1093/bioinformatics/btp149 (DOI)000265950600009 ()19289446 (PubMedID)
Available from: 2009-04-20 Created: 2009-04-20 Last updated: 2017-12-13Bibliographically approved
Andersson, R., Bruder, C. E., Piotrowski, A., Menzel, U., Nord, H., Sandgren, J., . . . Komorowski, J. (2008). A Segmental Maximum A Posteriori Approach to Genome-wide Copy Number Profiling. Bioinformatics, 24(6), 751-758
Open this publication in new window or tab >>A Segmental Maximum A Posteriori Approach to Genome-wide Copy Number Profiling
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2008 (English)In: Bioinformatics, ISSN 1367-4803, E-ISSN 1367-4811, Vol. 24, no 6, p. 751-758Article in journal (Other academic) Published
Abstract [en]

MOTIVATION: Copy number profiling methods aim at assigning DNA copy numbers to chromosomal regions using measurements from microarray-based comparative genomic hybridizations. Among the proposed methods to this end, Hidden Markov Model (HMM)-based approaches seem promising since DNA copy number transitions are naturally captured in the model. Current discrete-index HMM-based approaches do not, however, take into account heterogeneous information regarding the genomic overlap between clones. Moreover, the majority of existing methods are restricted to chromosome-wise analysis. RESULTS: We introduce a novel Segmental Maximum A Posteriori approach, SMAP, for DNA copy number profiling. Our method is based on discrete-index Hidden Markov Modeling and incorporates genomic distance and overlap between clones. We exploit a priori information through user-controllable parameterization that enables the identification of copy number deviations of various lengths and amplitudes. The model parameters may be inferred at a genome-wide scale to avoid overfitting of model parameters often resulting from chromosome-wise model inference. We report superior performances of SMAP on synthetic data when compared with two recent methods. When applied on our new experimental data, SMAP readily recognizes already known genetic aberrations including both large-scale regions with aberrant DNA copy number and changes affecting only single features on the array. We highlight the differences between the prediction of SMAP and the compared methods and show that SMAP accurately determines copy number changes and benefits from overlap consideration.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-13616 (URN)10.1093/bioinformatics/btn003 (DOI)000254010400003 ()18204059 (PubMedID)
Available from: 2008-08-21 Created: 2008-08-21 Last updated: 2017-12-11Bibliographically approved
Mikhail, F. M., Descartes, M., Piotrowski, A., Andersson, R., de Ståhl, T. D., Komorowski, J., . . . Carroll, A. J. (2007). A previously unrecognized microdeletion syndrome on chromosome 22 band q11.2 encompassing the BCR gene. American journal of medical genetics. Part A, 143A(18), 2178-2184
Open this publication in new window or tab >>A previously unrecognized microdeletion syndrome on chromosome 22 band q11.2 encompassing the BCR gene
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2007 (English)In: American journal of medical genetics. Part A, ISSN 1552-4825, Vol. 143A, no 18, p. 2178-2184Article in journal (Refereed) Published
Abstract [en]

Susceptibility of the chromosome 22q11.2 region to rearrangements has been recognized on the basis of common clinical disorders such as the DiGeorge/velocardiofacial syndrome (DG/VCFs). Recent evidence has implicated low-copy repeats (LCRs); also known as segmental duplications; on 22q as mediators of nonallelic homologous recombination (NAHR) that result in rearrangements of 22q11.2. It has been shown that both deletion and duplication events can occur as a result of NAHR caused by unequal crossover of LCRs. Here we report on the clinical, cytogenetic and array CGH studies of a 15-year-old Hispanic boy with history of learning and behavior problems. We suggest that he represents a previously unrecognized microdeletion syndrome on chromosome 22 band q11.2 just telomeric to the DG/VCFs typically deleted region and encompassing the BCR gene. Using a 32K BAC array CGH chip we were able to refine and precisely narrow the breakpoints of this microdeletion, which was estimated to be 1.55-1.92 Mb in size and to span approximately 20 genes. This microdeletion region is flanked by LCR clusters containing several modules with a very high degree of sequence homology (>95%), and therefore could play a causal role in its origin.

Keywords
chromosome 22, novel microdeletion, BCR gene, array CGH, 32K BAC array
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-13614 (URN)10.1002/ajmg.a.31882 (DOI)000249242200015 ()17676630 (PubMedID)
Available from: 2008-06-09 Created: 2008-06-09 Last updated: 2018-01-12Bibliographically approved
Mikhail, F. M., Sathienkijkanchai, A., Robin, N. H., Prucka, S., Biggerstaff, J. S., Komorowski, J., . . . Carroll, A. J. (2007). Overlapping phenotype of wolf-hirschhorn and beckwith-wiedemann syndromes in a girl with der(4)t(4; 1 1)(pter;pter). American Journal of Medical Genetics, Part A, 143(15), 1760-1766
Open this publication in new window or tab >>Overlapping phenotype of wolf-hirschhorn and beckwith-wiedemann syndromes in a girl with der(4)t(4; 1 1)(pter;pter)
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2007 (English)In: American Journal of Medical Genetics, Part A, ISSN 1552-4825, Vol. 143, no 15, p. 1760-1766Article in journal (Refereed) Published
Abstract [en]

We report on an 8-month-old girl with a novel unbalanced chromosomal rearrangement, consisting of a terminal deletion of 4p and a paternal duplication of terminal 11p. Each of these is associated with the well-known clinical phenotypes of Wolf-Hirschhorn syndrome (WHS) and Beckwith-Wiedemann syndrome (BWS), respectively. She presented for clinical evaluation of dysmorphic facial features, developmental delay, atrial septal defect (ASD), and left hydro-nephrosis. High-resolution cytogenetic analysis revealed a normal female karyotype, but subtelomeric fluorescence in situ hybridization (FISH) analysis revealed a der(4)t(4;11) (pter;pter). Both FISH and microarray CGH studies clearly demonstrated that the WHS critical regions 1 and 2 were deleted, and that the BWS imprinted domains (ID) 1 and 2 were duplicated on the der(4). Parental chromosome analysis revealed that the father carried a cryptic balanced t(4;11)(pter;pter). As expected, our patient manifests findings of both WHS (a growth retardation syndrome) and BWS (an overgrowth syndrome). We compare her unique phenotypic features with those that have been reported for both syndromes.

Keywords
Wolf-Hirschhorn syndrome (WHS), Beckwith-Wiedemann syndrome (BWS), fluorescence in situ hybridization (FISH), array CGH, unbalanced translocation, 4p, 11p
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-13613 (URN)10.1002/ajmg.a.31821 (DOI)000249144900013 ()17603794 (PubMedID)
Available from: 2008-06-09 Created: 2008-06-09 Last updated: 2011-01-25Bibliographically approved
Andersson, R., Vitoria, A., Maluszynski, J. & Komorowski, J. (2005). RoSy: A Rough Knowledge Base System. In: Rough Sets, Fuzzy Sets, Data Mining, and Granular Computing: 10th International Conference, RSFDGrC 2005, Regina, Canada, August 31 - September 3, 2005, Proceedings, Part II (pp. 48-58).
Open this publication in new window or tab >>RoSy: A Rough Knowledge Base System
2005 (English)In: Rough Sets, Fuzzy Sets, Data Mining, and Granular Computing: 10th International Conference, RSFDGrC 2005, Regina, Canada, August 31 - September 3, 2005, Proceedings, Part II, 2005, p. 48-58Conference paper, Published paper (Refereed)
Abstract [en]

This paper presents a user-oriented view of RoSy, a Rough Knowledge Base System. The system tackles two problems not fully answered by previous research: the ability to define rough sets in terms of other rough sets and incorporation of domain or expert knowledge. We describe two main components of RoSy: knowledge base creation and query answering. The former allows the user to create a knowledge base of rough concepts and checks that the definitions do not cause what we will call a model failure. The latter gives the user a possibility to query rough concepts defined in the knowledge base. The features of RoSy are described using examples. The system is currently available on a web site for online interactions.

Identifiers
urn:nbn:se:uu:diva-10986 (URN)doi:10.1007/11548706_6 (DOI)978-3-540-28660-8 (ISBN)
Available from: 2007-05-08 Created: 2007-05-08
Andersson, R., Enroth, S., Barbacioru, C., Reddy Bysani, M. S., Wallerman, O., Tuch, B., . . . Wadelius, C.Strand-based mixture modeling of nucleosome positioning in HepG2 cells and their regulatory dynamics in response to TGF-beta treatment.
Open this publication in new window or tab >>Strand-based mixture modeling of nucleosome positioning in HepG2 cells and their regulatory dynamics in response to TGF-beta treatment
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(English)Manuscript (preprint) (Other academic)
Identifiers
urn:nbn:se:uu:diva-130998 (URN)
Available from: 2010-09-20 Created: 2010-09-20 Last updated: 2010-11-11
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