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Xia, Wei, Associate ProfessorORCID iD iconorcid.org/0000-0002-7356-3002
Publications (10 of 128) Show all publications
Fu, L., Xiong, Y., Carlsson, G., Palmer, M., Örn, S., Zhu, W., . . . Xia, W. (2018). Biodegradable Si3N4bioceramic sintered with Sr, Mg and Si for spinal fusion:Surface characterization and biological evaluation. Applied Materials Today, 12, 260-275
Open this publication in new window or tab >>Biodegradable Si3N4bioceramic sintered with Sr, Mg and Si for spinal fusion:Surface characterization and biological evaluation
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2018 (English)In: Applied Materials Today, Vol. 12, p. 260-275-Article in journal (Refereed) Published
Abstract [en]

Silicon nitride (Si3N4) is an industrial ceramic used in spinal fusion and maxillofacial reconstructionbecause of its excellent mechanical properties and good biocompatibility. This study compares the sur-face properties, apatite formation ability, bacterial infection, cell-biomaterial interactions, and in vivotoxicity (zebrafish) of newly developed Si3N4 bioceramics (sintered with bioactive sintering additivesSrO, MgO and SiO2) with two standard biomaterials; titanium (Ti) and traditional Si3N4 bioceramics (sin-tered with standard sintering additives Al2O3 and Y2O3). In general, Si3N4 bioceramics (both the newlydeveloped and the traditional) displayed less in vitro bacterial affinity than Ti, which may arise fromdifferences in the surface properties between these two types of material. The newly developed Si3N4bioceramics developed lower biofilm coverage and thinner biofilm, compared to traditional Si3N4 bioce-ramics. The effects of ionic dissolution products (leach) on proliferation and differentiation of MC3T3-E1cell were also investigated. Ionic dissolution products containing moderate amount of Sr, Mg and Siions (approximately 4.72 mg/L, 3.26 mg/L and 3.67 mg/L, respectively) stimulated osteoblast prolifera-tion during the first 2 days in culture. Interestingly, ionic dissolution products from the traditional Si3N4bioceramics that contained small amount of Si and Y ions achieved the greatest stimulatory effect foralkaline phosphatase activity after 7 days culture. The toxicity of ionic dissolution products was investi-gated in a putative developmental biology model: zebrafish (Danio rerio). No toxicity, or developmentalabnormalities, was observed in zebrafish embryos exposed to ionic dissolution products, for up to 144 hpost fertilization. These newly developed Si3N4 bioceramics with bioactive sintering additives show greatpotential as orthopedic implants, for applications such as spinal fusion cages. Future work will focus onevaluation of the newly developed Si3N4 bioceramics using a large animal model.

Keywords
Si3N4 bioceramic, Spinal fusion, Biocompatibility, Bioactive ions, Zebrafish
National Category
Medical Materials
Identifiers
urn:nbn:se:uu:diva-356522 (URN)10.1016/j.apmt.2018.06.002 (DOI)
Available from: 2018-07-30 Created: 2018-07-30 Last updated: 2018-10-10Bibliographically approved
Mellgren, T., Qin, T., Öhman Mägi, C., Zhang, Y., Wu, B., Xia, W. & Engqvist, H. (2018). Calcium Phosphate Microspheres as a Delivery Vehicle for Tooth-Bleaching Agents. Journal of Dental Research, 97(3), 283-288
Open this publication in new window or tab >>Calcium Phosphate Microspheres as a Delivery Vehicle for Tooth-Bleaching Agents
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2018 (English)In: Journal of Dental Research, ISSN 0022-0345, E-ISSN 1544-0591, Vol. 97, no 3, p. 283-288Article in journal (Refereed) Published
Abstract [en]

Bleaching of vital teeth has become common practice in cosmetic dentistry today. Tooth sensitivity and demineralization of the enamel are, however, common side effects associated with hydrogen and carbamide peroxide bleaching. This study investigated if calcium phosphate microspheres, which have remineralizing properties, could be used as an additive without hindering the diffusion of the bleaching agent and if the spheres could be used as a carrier for carbamide peroxide. A remineralizing agent could increase the safety of bleaching and decrease the severity of its side effects. Comparisons between current hydrogen peroxide diffusion studies and previously published work are difficult since many studies include challenging-to-replicate conditions or lack reporting of important parameters. Hence, a diffusion model was designed by Wu Lab (School of Dentistry, University of California, Los Angeles) to measure the diffusion flux and determine the diffusivity of hydrogen peroxide. Physical parameters (e.g., diffusivity) could then be used for direct comparison to the results obtained by future studies. Three whitening gels with increasing amounts of spheres were formulated and tested with 2 commercially available whitening gels. The flux of hydrogen peroxide through 1-mm discs of bovine enamel was measured at steady-state conditions, and the diffusivity was calculated. The results showed that the spheres could be used as a carrier for carbamide peroxide and that the amount of spheres did not affect the diffusivity of peroxide through the enamel discs. Hence, the microspheres are considered promising as an additive to minimize side effects in bleaching gel formulation.

Place, publisher, year, edition, pages
SAGE PUBLICATIONS INC, 2018
Keywords
dental enamel, diffusion, peroxide, tooth whitening, tooth sensitivity, tooth remineralization
National Category
Dentistry Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-352988 (URN)10.1177/0022034517741295 (DOI)000429319800006 ()29125909 (PubMedID)
Funder
Swedish Research Council, 20135419
Available from: 2018-07-17 Created: 2018-07-17 Last updated: 2018-07-25Bibliographically approved
Sladkova, M., Cheng, J., Pujari-Palmer, M., Chen, S., Lin, C., Xia, W., . . . Maria de Peppo, G. (2018). Comparison of Decellularized Cow and Human Bone for Engineering Bone Grafts with human iPS Cells. Tissue Engineering. Part A
Open this publication in new window or tab >>Comparison of Decellularized Cow and Human Bone for Engineering Bone Grafts with human iPS Cells
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2018 (English)In: Tissue Engineering. Part A, ISSN 1937-3341, E-ISSN 1937-335XArticle in journal (Refereed) In press
National Category
Medical Materials
Identifiers
urn:nbn:se:uu:diva-358553 (URN)
Available from: 2018-08-30 Created: 2018-08-30 Last updated: 2018-08-30
Oroujeni, M., Kaboudin, B., Xia, W., Jönsson, P. & Ossipov, D. A. (2018). Conjugation of cyclodextrin to magnetic Fe3O4 nanoparticles via polydopamine coating for drug delivery. Progress in organic coatings, 114, 154-161
Open this publication in new window or tab >>Conjugation of cyclodextrin to magnetic Fe3O4 nanoparticles via polydopamine coating for drug delivery
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2018 (English)In: Progress in organic coatings, ISSN 0300-9440, E-ISSN 1873-331X, Vol. 114, p. 154-161Article in journal (Refereed) Published
Abstract [en]

Abstract

In this study, a novel magnetic nanocarrier for hydrophobic drugs (β-CD–PDA–MNPs) was fabricated using surface coating of Fe3O4 nanoparticles with polydopamine (PDA) followed by functionalization with 6-thio-β-cyclodextrin (6-thio-β-CD). The obtained magnetic nanoparticles were employed to investigate their interactions with diclofenac (DCF) as a model hydrophobic drug. The resulting β-CD–PDA–MNPs were characterized by various methods including transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), and vibrating sample magnetometry (VSM). The newly fabricated magnetic nanocarrier exhibited considerably higher drug loading capacity as compared for its analogue lacking CD ligands. Moreover, the release profile of DCF from β-CD–PDA–MNPs showed a burst release during the initial 8 h followed by the drug sustained release. Facile coating of magnetic nanoparticles with PDA was therefore a robust synthetic procedure for the conversion of the nanoparticles into a drug vehicle.

National Category
Other Physics Topics Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-354511 (URN)10.1016/j.porgcoat.2017.10.007 (DOI)
Available from: 2018-06-20 Created: 2018-06-20 Last updated: 2018-10-12Bibliographically approved
Tang, X.-l., Wu, J., Li, B.-l., Cui, S., Liu, H.-m., Yu, R.-t., . . . Xia, W. (2018). Near-infrared light-activated red-emitting upconverting nanoplatform for T-1-weighted magnetic resonance imaging and photodynamic therapy. Acta Biomaterialia, 74, 360-373
Open this publication in new window or tab >>Near-infrared light-activated red-emitting upconverting nanoplatform for T-1-weighted magnetic resonance imaging and photodynamic therapy
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2018 (English)In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 74, p. 360-373Article in journal (Refereed) Published
Abstract [en]

Photodynamic therapy (PDT) has increasingly become an efficient and attractive cancer treatment modality based on reactive oxygen species (ROS) that can induce tumor death after irradiation with ultraviolet or visible light. Herein, to overcome the limited tissue penetration in traditional PDT, a novel near-infrared (NIR) light-activated NaScF4: 40% Yb, 2% Er@CaF2 upconversion nanoparticle (rUCNP) is successfully designed and synthesized. Chlorin e6, a photosensitizer and a chelating agent for Mn2+, is loaded into human serum albumin (HSA) that further conjugates onto rUCNPs. To increase the ability to target glioma tumor, an acyclic Arg-Gly-Asp peptide (cRGDyK) is linked to rUCNPs@HSA(Ce6-Mn). This nanoplatform enables efficient adsorption and conversion of NIR light (980 nm) into bright red emission (660 nm), which can trigger the photosensitizer Ce6-Mn complex for PDT and T-1-weighted magnetic resonance imaging (T-1-weighted MRI) for glioma diagnosis. Our in vitro and in vivo experiments demonstrate that NIR light-activated and glioma tumor-targeted PDT can generate large amounts of intracellular ROS that induce U87 cell apoptosis and suppress glioma tumor growth owing to the deep tissue penetration of irradiated light and excellent tumor-targeting ability. Thus, this nanoplatform holds potential for applications in T-1-weighted MRI diagnosis and PDT of glioma for antitumor therapy. Statement of Significance A near-infrared (NIR) light-activated nanoplatform for photodynamic therapy (PDT) was designed and synthesized. The Red-to-Green (RIG) ratio of NaScF4: 40% Yb, 2% Er almost reached 9, a value that was much higher than that of a traditional Yb/Er-codoped upconversion nanoparticle (rUCNP). By depositing a CaF2 shell, the red-emission intensities of the rUCNPs were seven times strong as that of NaScF4: 40% Yb, 2% Er. The enhanced red-emitting rUCNPs could be applied in many fields such as bioimaging, controlled release, and real-time diagnosis. The nanoplatform had a strong active glioma-targeting ability, and all results achieved on subcutaneous glioma demonstrated that our NIR light-activated redemitting upconverting nanoplatform was efficient for PDT. By loading Ce6-Mn complex into rUCNPs@HSA-RGD, the nanoplatform could be used as a T-1-weighted magnetic resonance imaging agent for tumor diagnosis.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD, 2018
Keywords
Upconversion nanoparticles, Enhanced red-emission, Magnetic resonance imaging, Tumor targeting, Photodynamic therapy
National Category
Cancer and Oncology Biomaterials Science Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-361103 (URN)10.1016/j.actbio.2018.05.017 (DOI)000437998200028 ()29763715 (PubMedID)
Available from: 2018-09-21 Created: 2018-09-21 Last updated: 2018-10-09Bibliographically approved
Tang, X., Jun, W., Benlan, L., Sheng, C., Hongmei, L., Rutong, Y., . . . Xia, W. (2018). Near-infrared light-activated red-emitting upconverting nanoplatform for T1-weighted magnetic resonance imaging and photodynamic therapy. Acta Biomaterialia
Open this publication in new window or tab >>Near-infrared light-activated red-emitting upconverting nanoplatform for T1-weighted magnetic resonance imaging and photodynamic therapy
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2018 (English)In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568Article in journal (Refereed) Published
Abstract [en]

Abstract

Photodynamic therapy (PDT) has increasingly become an efficient and attractive cancer treatment modality based on reactive oxygen species (ROS) that can induce tumor death after irradiation with ultraviolet or visible light. Herein, to overcome the limited tissue penetration in traditional PDT, a novel near-infrared (NIR) light-activated NaScF4: 40% Yb, 2% Er@CaF2 upconversion nanoparticle (rUCNP) is successfully designed and synthesized. Chlorin e6, a photosensitizer and a chelating agent for Mn2+, is loaded into human serum albumin (HSA) that further conjugates onto rUCNPs. To increase the ability to target glioma tumor, an acyclic Arg-Gly-Asp peptide (cRGDyK) is linked to rUCNPs@HSA(Ce6-Mn). This nanoplatform enables efficient adsorption and conversion of NIR light (980 nm) into bright red emission (660 nm), which can trigger the photosensitizer Ce6-Mn complex for PDT and T1-weighted magnetic resonance imaging (T1-weighted MRI) for glioma diagnosis. Our in vitro and in vivo experiments demonstrate that NIR light-activated and glioma tumor-targeted PDT can generate large amounts of intracellular ROS that induce U87 cell apoptosis and suppress glioma tumor growth owing to the deep tissue penetration of irradiated light and excellent tumor-targeting ability. Thus, this nanoplatform holds potential for applications in T1-weighted MRI diagnosis and PDT of glioma for antitumor therapy.

STATEMENT OF SIGNIFICANCE:

A near-infrared (NIR) light-activated nanoplatform for photodynamic therapy (PDT) was designed and synthesized. The Red-to-Green (R/G) ratio of NaScF4: 40% Yb, 2% Er almost reached 9, a value that was much higher than that of a traditional Yb/Er-codoped upconversion nanoparticle (rUCNP). By depositing a CaF2 shell, the red-emission intensities of the rUCNPs were seven times strong as that of NaScF4: 40% Yb, 2% Er. The enhanced red-emitting rUCNPs could be applied in many fields such as bioimaging, controlled release, and real-time diagnosis. The nanoplatform had a strong active glioma-targeting ability, and all results achieved on subcutaneous glioma demonstrated that our NIR light-activated red-emitting upconverting nanoplatform was efficient for PDT. By loading Ce6-Mn complex into rUCNPs@HSA-RGD, the nanoplatform could be used as a T1-weighted magnetic resonance imaging agent for tumor diagnosis.

Copyright © 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

KEYWORDS:

Enhanced red-emission; Magnetic resonance imaging; Photodynamic therapy; Tumor targeting; Upconversion nanoparticles

National Category
Biomaterials Science Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-354507 (URN)10.1016/j.actbio.2018.05.017 (DOI)
Available from: 2018-06-20 Created: 2018-06-20 Last updated: 2018-10-12Bibliographically approved
Cui, S., Wang, X., Zhang, X., Xia, W., Tang, X., Lin, B., . . . Shen, X. (2018). Preparation of magnetic MnFe2O4-Cellulose aerogel composite and its kinetics and thermodynamics of Cu(II) adsorption. Cellulose (London), 25(1), 735-751
Open this publication in new window or tab >>Preparation of magnetic MnFe2O4-Cellulose aerogel composite and its kinetics and thermodynamics of Cu(II) adsorption
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2018 (English)In: Cellulose (London), ISSN 0969-0239, E-ISSN 1572-882X, Vol. 25, no 1, p. 735-751Article in journal (Refereed) Published
Abstract [en]

In this paper, a MnFe2O4-Cellulose magnetic composite aerogel (MnCA) with high adsorption capacity was fabricated by in situ incorporating MnFe2O4 to regenerated cellulose hydrogel matrix, followed by CO2 supercritical drying. A green synthetic strategy was performed by using renewable cellulose materials, environmentally benign cellulose solvents and facile synthetic conditions. The results showed that the obtained magnetic cellulose aerogel had a continuous and tiered three dimensional network with interconnected fibrils of about 30 nm in width, which was similar to those of cellulose aerogel prepared from NaOH/urea solution via CO2 supercritical drying. Meanwhile, they had high specific surface areas of 236-288 m(2)/g and total pore volume of 0.55-0.88 cm(3)/g. In addition, the hybrid aerogel showed superparamagnetism with maximum saturation magnetization reaching up to 18.53 emu/g. The magnetic nanocomposite aerogel could be used for biological and environmental applications. The adsorption test showed that MnCA had rapid adsorption rate and excellent adsorption ability of removing heavy metal ions in aqueous solution which could attain to 63.3 mg/g within 100 min. Moreover, all the composite aerogels exhibited good reusability and could be easily reused from the water after adsorption.

Place, publisher, year, edition, pages
SPRINGER, 2018
Keywords
Magnetic aerogel, MnFe2O4-Cellulose, Composites, Copper ion, Adsorption
National Category
Materials Engineering
Identifiers
urn:nbn:se:uu:diva-342464 (URN)10.1007/s10570-017-1598-x (DOI)000419902600059 ()
Available from: 2018-02-26 Created: 2018-02-26 Last updated: 2018-02-26Bibliographically approved
Fu, L., Engqvist, H. & Xia, W. (2018). Spark plasma sintering of biodegradable Si3N4 bioceramic with Sr, Mg and Si as sintering additives for spinal fusion. Journal of the European Ceramic Society, 38(4), 2110-2119
Open this publication in new window or tab >>Spark plasma sintering of biodegradable Si3N4 bioceramic with Sr, Mg and Si as sintering additives for spinal fusion
2018 (English)In: Journal of the European Ceramic Society, ISSN 0955-2219, E-ISSN 1873-619X, Vol. 38, no 4, p. 2110-2119Article in journal (Refereed) Published
Abstract [en]

Silicon nitride (Si3N4) bioceramics with standard sintering additives (Al2O3 and Y2O3) are used in spinal fusion. Alternative Si3N4 bioceramics with biologically beneficial sintering additives could lead to improved osseoin- tegrative properties. The aim of this study is to obtain dense and strong Si3N4 bioceramics by using SrO, MgO and SiO2 as sintering additives, and evaluate the effect of these sintering additives on microstructures and properties of Si3N4 bioceramics. Raw powders with 10 wt% and 18 wt% sintering additives were sintered by spark plasma sintering. Samples sintered at 1750 °C, with an applied pressure of 60 MPa and a holding time of 3 min, showed the highest content of β-Si3N4 (94.9%). The mechanical properties of the developed Si3N4 bio- ceramics are comparable to the mechanical properties of currently used structural Si3N4 ceramics sintered with standard sintering additives (Al2O3 and Y2O3). The highest flexural strength of the developed Si3N4 bioceramics reached 1079 MPa. Ion release results showed that Sr2+,Mg2+ and Si4+ ions kept leaching out within 10 days’ immersion. The degradable Si3N4 bioceramics with adequate strength and biologically beneficial sintering ad- ditives show the promise for load bearing biomedical applications, such as spinal fusion.

National Category
Natural Sciences Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-331879 (URN)10.1016/j.jeurceramsoc.2017.10.003 (DOI)000424716700136 ()
Funder
Carl Tryggers foundation
Available from: 2017-10-19 Created: 2017-10-19 Last updated: 2018-08-12Bibliographically approved
Garbani, M., Xia, W., Rhyner, C., Prati, M., Scheynius, A., Malissen, B., . . . Terhorst-Molawi, D. (2017). Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice. Allergy. European Journal of Allergy and Clinical Immunology, 72(4), 570-578
Open this publication in new window or tab >>Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice
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2017 (English)In: Allergy. European Journal of Allergy and Clinical Immunology, ISSN 0105-4538, E-ISSN 1398-9995, Vol. 72, no 4, p. 570-578Article in journal (Refereed) Published
Abstract [en]

Background

Immunomodulatory interventions play a key role in the treatment of infections and cancer as well as allergic diseases. Adjuvants such as micro- and nanoparticles are often added to immunomodulatory therapies to enhance the triggered immune response. Here, we report the immunological assessment of novel and economically manufactured microparticle adjuvants, namely strontium-doped hydroxyapatite porous spheres (SHAS), which we suggest for the use as adjuvant and carrier in allergen-specific immunotherapy (ASIT).

Methods and Results

Scanning electron microscopy revealed that the synthesis procedure developed for the production of SHAS results in a highly homogeneous population of spheres. SHAS bound and released proteins such as ovalbumin (OVA) or the major cat allergen Fel d 1. SHAS-OVA were taken up by human monocyte-derived dendritic cells (mdDCs) and murine DCs and did not have any necrotic or apoptotic effects even at high densities. In a murine model of ASIT for allergic asthmatic inflammation we found that OVA released from subcutaneously injected SHAS-OVA led to a sustained stimulation of both CD4+ and CD8+ T-cells. ASIT with SHAS-OVA as compared to soluble OVA resulted in similar humoral responses but in a higher efficacy as assessed by symptom scoring.

Conclusion

We conclude that SHAS may constitute a suitable carrier and adjuvant for ASIT with great potential due to its unique protein-binding properties.

National Category
Medical Materials
Identifiers
urn:nbn:se:uu:diva-310479 (URN)10.1111/all.13041 (DOI)000397489400007 ()27590538 (PubMedID)
Funder
Swedish Research Council, 2013-5419
Available from: 2016-12-16 Created: 2016-12-16 Last updated: 2017-05-15Bibliographically approved
Ting, M., Jefferies, S. R., Xia, W., Engqvist, H. & Suzuki, J. B. (2017). Classification and Effects of Implant Surface Modification on the Bone: Human Cell-Based In Vitro Studies. Journal of Oral Implantology, 43(1), 58-83
Open this publication in new window or tab >>Classification and Effects of Implant Surface Modification on the Bone: Human Cell-Based In Vitro Studies
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2017 (English)In: Journal of Oral Implantology, ISSN 0160-6972, E-ISSN 1548-1336, Vol. 43, no 1, p. 58-83Article, review/survey (Refereed) Published
Abstract [en]

Implant surfaces are continuously being improved to achieve faster osseointegration and a stronger bone to implant interface. This review will present the various implant surfaces, the parameters for implant surface characterization, and the corresponding in vitro human cell-based studies determining the strength and quality of the bone-implant contact. These in vitro cell-based studies are the basis for animal and clinical studies and are the prelude to further reviews on how these surfaces would perform when subjected to the oral environment and functional loading.

Place, publisher, year, edition, pages
ALLEN PRESS INC, 2017
Keywords
titanium, dental implant, implant surface morphology, osseointegration, surface topography
National Category
Medical and Health Sciences Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-320997 (URN)10.1563/aaid-joi-D-16-00079 (DOI)000395366200011 ()27897464 (PubMedID)
Available from: 2017-04-28 Created: 2017-04-28 Last updated: 2017-04-28Bibliographically approved
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ORCID iD: ORCID iD iconorcid.org/0000-0002-7356-3002

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