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Xia, Wei, Associate ProfessorORCID iD iconorcid.org/0000-0002-7356-3002
Publications (10 of 133) Show all publications
Xia, W., Cai, B., Engqvist, H. & Bredenberg, S. (2018). A transdermal drug administration device. JP2018513121A.
Open this publication in new window or tab >>A transdermal drug administration device
2018 (English)Patent (Other (popular science, discussion, etc.))
National Category
Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-372236 (URN)
Patent
JP2018513121A
Available from: 2019-01-07 Created: 2019-01-07 Last updated: 2019-01-07
Yanlun, Z., Lingzhi, K., Fatemeh, F., Xia, W., Chang, J., Yaohua, H. & Haiyan, L. (2018). An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration. Biomaterials, 192, 149-158
Open this publication in new window or tab >>An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration
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2018 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 192, p. 149-158Article in journal (Refereed) Published
Abstract [en]

Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge. In this study, a construct containing an injectable continuous stratified scaffold and multiple cell systems was designed for enhancing osteochondral regeneration. Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration. The continuous SA phase and the stratified structure enable the scaffold to mimic the natural osteochondral structure. In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function. Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed. This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.

National Category
Medical Materials
Identifiers
urn:nbn:se:uu:diva-366108 (URN)
Available from: 2018-11-16 Created: 2018-11-16 Last updated: 2019-01-17Bibliographically approved
Vallhov, H., Xia, W., Engqvist, H. & Scheynius, A. (2018). Bioceramic microneedle arrays are able to deliver OVA to dendritic cells in human skin. Journal of materials chemistry. B, 6(42), 6808-6816
Open this publication in new window or tab >>Bioceramic microneedle arrays are able to deliver OVA to dendritic cells in human skin
2018 (English)In: Journal of materials chemistry. B, ISSN 2050-750X, E-ISSN 2050-7518, Vol. 6, no 42, p. 6808-6816Article in journal (Refereed) Published
Abstract [en]

Microneedle-based vaccination into skin has several advantages over vaccination using conventional needles for intramuscular or subcutaneous injections. Microneedle (MN) arrays allow the vaccine to be delivered in a minimally invasive manner and directly into the skin, whereby the skin's superficial immune cells are not by-passed. Additionally, a systemic distribution of the vaccine may be avoided, which implies less side effects and less amount of vaccine needed. For a successful delivery, the needles need to penetrate the stratum corneum and reach the potent network of antigen-presenting dendritic cells (DCs). In this study, we evaluated patches covered with biodegradable ceramic (calcium sulphate) MNs with a tip diameter of approximately 3 μm and with two different lengths (300 and 600 μm) for their ability to penetrate and transfer the model allergen ovalbumin (OVA) into epidermis. MNs with a length of 600 μm (MN-600) and a volume average pore size of 12 ± 1 μm were more efficient in crossing the stratum corneum and to deliver OVA into CD1a+ DCs residing in the epidermis of human ex vivo skin, in comparison to MNs with a length of 300 μm. Quantitative in vitro release studies showed that approximately 90% of the loaded OVA could be released from MN-600 within 1 h. These findings support the further development of ceramic MNs for transcutaneous immunization.

National Category
Medical Materials
Identifiers
urn:nbn:se:uu:diva-366114 (URN)10.1039/c8tb01476k (DOI)000451177200011 ()
Funder
Swedish Research CouncilCancer and Allergy FoundationThe Karolinska Institutet's Research Foundation
Available from: 2018-11-16 Created: 2018-11-16 Last updated: 2019-01-07Bibliographically approved
Fu, L., Xiong, Y., Carlsson, G., Palmer, M., Örn, S., Zhu, W., . . . Xia, W. (2018). Biodegradable Si3N4bioceramic sintered with Sr, Mg and Si for spinal fusion:Surface characterization and biological evaluation. Applied Materials Today, 12, 260-275
Open this publication in new window or tab >>Biodegradable Si3N4bioceramic sintered with Sr, Mg and Si for spinal fusion:Surface characterization and biological evaluation
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2018 (English)In: Applied Materials Today, Vol. 12, p. 260-275-Article in journal (Refereed) Published
Abstract [en]

Silicon nitride (Si3N4) is an industrial ceramic used in spinal fusion and maxillofacial reconstructionbecause of its excellent mechanical properties and good biocompatibility. This study compares the sur-face properties, apatite formation ability, bacterial infection, cell-biomaterial interactions, and in vivotoxicity (zebrafish) of newly developed Si3N4 bioceramics (sintered with bioactive sintering additivesSrO, MgO and SiO2) with two standard biomaterials; titanium (Ti) and traditional Si3N4 bioceramics (sin-tered with standard sintering additives Al2O3 and Y2O3). In general, Si3N4 bioceramics (both the newlydeveloped and the traditional) displayed less in vitro bacterial affinity than Ti, which may arise fromdifferences in the surface properties between these two types of material. The newly developed Si3N4bioceramics developed lower biofilm coverage and thinner biofilm, compared to traditional Si3N4 bioce-ramics. The effects of ionic dissolution products (leach) on proliferation and differentiation of MC3T3-E1cell were also investigated. Ionic dissolution products containing moderate amount of Sr, Mg and Siions (approximately 4.72 mg/L, 3.26 mg/L and 3.67 mg/L, respectively) stimulated osteoblast prolifera-tion during the first 2 days in culture. Interestingly, ionic dissolution products from the traditional Si3N4bioceramics that contained small amount of Si and Y ions achieved the greatest stimulatory effect foralkaline phosphatase activity after 7 days culture. The toxicity of ionic dissolution products was investi-gated in a putative developmental biology model: zebrafish (Danio rerio). No toxicity, or developmentalabnormalities, was observed in zebrafish embryos exposed to ionic dissolution products, for up to 144 hpost fertilization. These newly developed Si3N4 bioceramics with bioactive sintering additives show greatpotential as orthopedic implants, for applications such as spinal fusion cages. Future work will focus onevaluation of the newly developed Si3N4 bioceramics using a large animal model.

Keywords
Si3N4 bioceramic, Spinal fusion, Biocompatibility, Bioactive ions, Zebrafish
National Category
Medical Materials
Identifiers
urn:nbn:se:uu:diva-356522 (URN)10.1016/j.apmt.2018.06.002 (DOI)
Available from: 2018-07-30 Created: 2018-07-30 Last updated: 2018-11-16Bibliographically approved
Mellgren, T., Qin, T., Öhman Mägi, C., Zhang, Y., Wu, B., Xia, W. & Engqvist, H. (2018). Calcium Phosphate Microspheres as a Delivery Vehicle for Tooth-Bleaching Agents. Journal of Dental Research, 97(3), 283-288
Open this publication in new window or tab >>Calcium Phosphate Microspheres as a Delivery Vehicle for Tooth-Bleaching Agents
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2018 (English)In: Journal of Dental Research, ISSN 0022-0345, E-ISSN 1544-0591, Vol. 97, no 3, p. 283-288Article in journal (Refereed) Published
Abstract [en]

Bleaching of vital teeth has become common practice in cosmetic dentistry today. Tooth sensitivity and demineralization of the enamel are, however, common side effects associated with hydrogen and carbamide peroxide bleaching. This study investigated if calcium phosphate microspheres, which have remineralizing properties, could be used as an additive without hindering the diffusion of the bleaching agent and if the spheres could be used as a carrier for carbamide peroxide. A remineralizing agent could increase the safety of bleaching and decrease the severity of its side effects. Comparisons between current hydrogen peroxide diffusion studies and previously published work are difficult since many studies include challenging-to-replicate conditions or lack reporting of important parameters. Hence, a diffusion model was designed by Wu Lab (School of Dentistry, University of California, Los Angeles) to measure the diffusion flux and determine the diffusivity of hydrogen peroxide. Physical parameters (e.g., diffusivity) could then be used for direct comparison to the results obtained by future studies. Three whitening gels with increasing amounts of spheres were formulated and tested with 2 commercially available whitening gels. The flux of hydrogen peroxide through 1-mm discs of bovine enamel was measured at steady-state conditions, and the diffusivity was calculated. The results showed that the spheres could be used as a carrier for carbamide peroxide and that the amount of spheres did not affect the diffusivity of peroxide through the enamel discs. Hence, the microspheres are considered promising as an additive to minimize side effects in bleaching gel formulation.

Place, publisher, year, edition, pages
SAGE PUBLICATIONS INC, 2018
Keywords
dental enamel, diffusion, peroxide, tooth whitening, tooth sensitivity, tooth remineralization
National Category
Dentistry Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-352988 (URN)10.1177/0022034517741295 (DOI)000429319800006 ()29125909 (PubMedID)
Funder
Swedish Research Council, 20135419
Available from: 2018-07-17 Created: 2018-07-17 Last updated: 2018-07-25Bibliographically approved
Sladkova, M., Cheng, J., Palmer, M., Chen, S., Lin, C., Xia, W., . . . de Peppo, G. M. (2018). Comparison of Decellularized Cow and Human Bone for Engineering Bone Grafts with Human Induced Pluripotent Stem Cells. Tissue Engineering. Part A
Open this publication in new window or tab >>Comparison of Decellularized Cow and Human Bone for Engineering Bone Grafts with Human Induced Pluripotent Stem Cells
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2018 (English)In: Tissue Engineering. Part A, ISSN 1937-3341, E-ISSN 1937-335XArticle in journal (Refereed) Published
Abstract [en]

Bone engineering makes it possible to grow unlimited amounts of viable tissue products for basic and applied research, and for clinical applications. A common trend in tissue engineering is the use of decellularized tissue matrices as scaffolding materials, which display structural, mechanical, and biological attributes typical of the native tissue. Due to the limited availability and high cost of human samples, decellularized tissue matrices are typically derived from animal sources. It is unclear, however, whether interspecies differences in tissue parameters will influence the quality of tissue grafts that are engineered using human stem cells. In this study, decellularized cow and human bone scaffolds were compared for engineering bone grafts using human induced pluripotent stem cell-derived mesodermal progenitor cells. After seeding, the cell-scaffold constructs were cultured for 5 weeks in osteogenic medium under dynamic conditions in perfusion bioreactors. The architectural and chemical properties of the scaffolds were studied using microscopic, spectroscopic, and thermogravimetric techniques, while cell behavior and formation of mineralized tissue were assessed using a combination of molecular assays, histological methods, and imaging technologies. The results show that while scaffolds derived from cow and human bone differ somewhat in architecture and composition, both equally support cell viability, tissue growth, and formation of a mineralized bone matrix. Taken together, the results suggest that scaffolds derived from cow bone represent a suitable and convenient alternative to engineer human bone grafts for various biomedical applications.

Place, publisher, year, edition, pages
MARY ANN LIEBERT, INC, 2018
Keywords
biomaterial scaffold, bone engineering, induced pluripotent stem cells, mesenchymal stem cells, osteogenic differentiation, tissue decellularization
National Category
Biomaterials Science Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-369891 (URN)10.1089/ten.tea.2018.0149 (DOI)000448565100001 ()
Funder
EU, FP7, Seventh Framework Programme
Note

De 2 första författarna delar förstaförfattarskapet.

Available from: 2018-12-19 Created: 2018-12-19 Last updated: 2019-01-02Bibliographically approved
Oroujeni, M., Kaboudin, B., Xia, W., Jönsson, P. & Ossipov, D. A. (2018). Conjugation of cyclodextrin to magnetic Fe3O4 nanoparticles via polydopamine coating for drug delivery. Progress in organic coatings, 114, 154-161
Open this publication in new window or tab >>Conjugation of cyclodextrin to magnetic Fe3O4 nanoparticles via polydopamine coating for drug delivery
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2018 (English)In: Progress in organic coatings, ISSN 0300-9440, E-ISSN 1873-331X, Vol. 114, p. 154-161Article in journal (Refereed) Published
Abstract [en]

Abstract

In this study, a novel magnetic nanocarrier for hydrophobic drugs (β-CD–PDA–MNPs) was fabricated using surface coating of Fe3O4 nanoparticles with polydopamine (PDA) followed by functionalization with 6-thio-β-cyclodextrin (6-thio-β-CD). The obtained magnetic nanoparticles were employed to investigate their interactions with diclofenac (DCF) as a model hydrophobic drug. The resulting β-CD–PDA–MNPs were characterized by various methods including transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), and vibrating sample magnetometry (VSM). The newly fabricated magnetic nanocarrier exhibited considerably higher drug loading capacity as compared for its analogue lacking CD ligands. Moreover, the release profile of DCF from β-CD–PDA–MNPs showed a burst release during the initial 8 h followed by the drug sustained release. Facile coating of magnetic nanoparticles with PDA was therefore a robust synthetic procedure for the conversion of the nanoparticles into a drug vehicle.

National Category
Other Physics Topics Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-354511 (URN)10.1016/j.porgcoat.2017.10.007 (DOI)
Available from: 2018-06-20 Created: 2018-06-20 Last updated: 2018-10-12Bibliographically approved
Sun, W., Zhou, Y., Zhang, X., Xia, W., Xu, Y. & Lin, K. (2018). Injectable nano-structured silicon-containing hydroxyapatite microspheres with enhanced osteogenic differentiation and angiogenic factor expression. Ceramics International, 44(16), 20457-20464
Open this publication in new window or tab >>Injectable nano-structured silicon-containing hydroxyapatite microspheres with enhanced osteogenic differentiation and angiogenic factor expression
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2018 (English)In: Ceramics International, ISSN 0272-8842, E-ISSN 1873-3956, Vol. 44, no 16, p. 20457-20464Article in journal (Refereed) Published
Abstract [en]

Injectable bioactive ceramics with excellent osteogenesis play important roles in bone regeneration field. In this study, we creatively fabricated the injectable nano-structured silicon-containing hydroxyapatite (Si-HAp) microspheres in diameter of 70–100 µm via hydrothermal treatment of calcium silicate (CaSiO3) microspheres in Na3PO4 aqueous solution. The fabricated Si-HAp microspheres were constructed by nano-rods with a diameter of 100 nm and length up to 1.5 µm. Comparing with the pure HAp microspheres, the obtained nano-structured Si-HAp microspheres exhibited excellent protein loading properties and sustained protein release capacities. Most importantly, the Si-substitution could apparently enhance the proliferation, osteogenic differentiation and the genes expression of angiogenic factors of rat bone marrow mesenchymal stem cells (rBMSCs). These all indicated that the nano-structured Si-HAp microspheres fabricated via hydrothermal transformation method might be used as promising injectable bioactive biomaterials and drug deliveries for bone regeneration.

National Category
Medical Materials
Identifiers
urn:nbn:se:uu:diva-358550 (URN)10.1016/j.ceramint.2018.08.040 (DOI)000447101500181 ()
Available from: 2018-08-30 Created: 2018-08-30 Last updated: 2018-12-10Bibliographically approved
Tang, X.-l., Wu, J., Li, B.-l., Cui, S., Liu, H.-m., Yu, R.-t., . . . Xia, W. (2018). Near-infrared light-activated red-emitting upconverting nanoplatform for T-1-weighted magnetic resonance imaging and photodynamic therapy. Acta Biomaterialia, 74, 360-373
Open this publication in new window or tab >>Near-infrared light-activated red-emitting upconverting nanoplatform for T-1-weighted magnetic resonance imaging and photodynamic therapy
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2018 (English)In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 74, p. 360-373Article in journal (Refereed) Published
Abstract [en]

Photodynamic therapy (PDT) has increasingly become an efficient and attractive cancer treatment modality based on reactive oxygen species (ROS) that can induce tumor death after irradiation with ultraviolet or visible light. Herein, to overcome the limited tissue penetration in traditional PDT, a novel near-infrared (NIR) light-activated NaScF4: 40% Yb, 2% Er@CaF2 upconversion nanoparticle (rUCNP) is successfully designed and synthesized. Chlorin e6, a photosensitizer and a chelating agent for Mn2+, is loaded into human serum albumin (HSA) that further conjugates onto rUCNPs. To increase the ability to target glioma tumor, an acyclic Arg-Gly-Asp peptide (cRGDyK) is linked to rUCNPs@HSA(Ce6-Mn). This nanoplatform enables efficient adsorption and conversion of NIR light (980 nm) into bright red emission (660 nm), which can trigger the photosensitizer Ce6-Mn complex for PDT and T-1-weighted magnetic resonance imaging (T-1-weighted MRI) for glioma diagnosis. Our in vitro and in vivo experiments demonstrate that NIR light-activated and glioma tumor-targeted PDT can generate large amounts of intracellular ROS that induce U87 cell apoptosis and suppress glioma tumor growth owing to the deep tissue penetration of irradiated light and excellent tumor-targeting ability. Thus, this nanoplatform holds potential for applications in T-1-weighted MRI diagnosis and PDT of glioma for antitumor therapy. Statement of Significance A near-infrared (NIR) light-activated nanoplatform for photodynamic therapy (PDT) was designed and synthesized. The Red-to-Green (RIG) ratio of NaScF4: 40% Yb, 2% Er almost reached 9, a value that was much higher than that of a traditional Yb/Er-codoped upconversion nanoparticle (rUCNP). By depositing a CaF2 shell, the red-emission intensities of the rUCNPs were seven times strong as that of NaScF4: 40% Yb, 2% Er. The enhanced red-emitting rUCNPs could be applied in many fields such as bioimaging, controlled release, and real-time diagnosis. The nanoplatform had a strong active glioma-targeting ability, and all results achieved on subcutaneous glioma demonstrated that our NIR light-activated redemitting upconverting nanoplatform was efficient for PDT. By loading Ce6-Mn complex into rUCNPs@HSA-RGD, the nanoplatform could be used as a T-1-weighted magnetic resonance imaging agent for tumor diagnosis.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD, 2018
Keywords
Upconversion nanoparticles, Enhanced red-emission, Magnetic resonance imaging, Tumor targeting, Photodynamic therapy
National Category
Cancer and Oncology Biomaterials Science Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-361103 (URN)10.1016/j.actbio.2018.05.017 (DOI)000437998200028 ()29763715 (PubMedID)
Available from: 2018-09-21 Created: 2018-09-21 Last updated: 2018-10-09Bibliographically approved
Cui, S., Wang, X., Zhang, X., Xia, W., Tang, X., Lin, B., . . . Shen, X. (2018). Preparation of magnetic MnFe2O4-Cellulose aerogel composite and its kinetics and thermodynamics of Cu(II) adsorption. Cellulose (London), 25(1), 735-751
Open this publication in new window or tab >>Preparation of magnetic MnFe2O4-Cellulose aerogel composite and its kinetics and thermodynamics of Cu(II) adsorption
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2018 (English)In: Cellulose (London), ISSN 0969-0239, E-ISSN 1572-882X, Vol. 25, no 1, p. 735-751Article in journal (Refereed) Published
Abstract [en]

In this paper, a MnFe2O4-Cellulose magnetic composite aerogel (MnCA) with high adsorption capacity was fabricated by in situ incorporating MnFe2O4 to regenerated cellulose hydrogel matrix, followed by CO2 supercritical drying. A green synthetic strategy was performed by using renewable cellulose materials, environmentally benign cellulose solvents and facile synthetic conditions. The results showed that the obtained magnetic cellulose aerogel had a continuous and tiered three dimensional network with interconnected fibrils of about 30 nm in width, which was similar to those of cellulose aerogel prepared from NaOH/urea solution via CO2 supercritical drying. Meanwhile, they had high specific surface areas of 236-288 m(2)/g and total pore volume of 0.55-0.88 cm(3)/g. In addition, the hybrid aerogel showed superparamagnetism with maximum saturation magnetization reaching up to 18.53 emu/g. The magnetic nanocomposite aerogel could be used for biological and environmental applications. The adsorption test showed that MnCA had rapid adsorption rate and excellent adsorption ability of removing heavy metal ions in aqueous solution which could attain to 63.3 mg/g within 100 min. Moreover, all the composite aerogels exhibited good reusability and could be easily reused from the water after adsorption.

Place, publisher, year, edition, pages
SPRINGER, 2018
Keywords
Magnetic aerogel, MnFe2O4-Cellulose, Composites, Copper ion, Adsorption
National Category
Materials Engineering
Identifiers
urn:nbn:se:uu:diva-342464 (URN)10.1007/s10570-017-1598-x (DOI)000419902600059 ()
Available from: 2018-02-26 Created: 2018-02-26 Last updated: 2018-02-26Bibliographically approved
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ORCID iD: ORCID iD iconorcid.org/0000-0002-7356-3002

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