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Xia, Wei, Associate ProfessorORCID iD iconorcid.org/0000-0002-7356-3002
Publications (10 of 140) Show all publications
Zhu, W., Ma, Q., Borg, S., Öhman Mägi, C., Weng, X., Engqvist, H. & Xia, W. (2019). Cemented injectable multi-phased porous bone grafts for the treatment of femoral head necrosis. Journal of materials chemistry. B, 7(18), 2997-3006
Open this publication in new window or tab >>Cemented injectable multi-phased porous bone grafts for the treatment of femoral head necrosis
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2019 (English)In: Journal of materials chemistry. B, ISSN 2050-750X, E-ISSN 2050-7518, Vol. 7, no 18, p. 2997-3006Article in journal (Refereed) Published
Abstract [en]

Femoral head necrosis (FHN) can induce musculoskeletal disability. It presents a challenge from diagnostic and therapeutic points of view. Open surgery for the treatment of FHN is not an optimal route. To minimize the surgery window, an injectable material with a porous structure and bioactive nature is preferred. The fabrication of an injectable porous bone graft via a simple route was the aim of our study. Therefore, cemented multi-phased calcium phosphate porous granules have been studied with varied phase compositions, pore sizes and porosities, and degradation rates. Granules templated by PEG 100-600 mu m were chosen for cell toxicity and in vitro osteogenic potential testing. Rabbits, making up a femoral head necrosis model, were implanted with granule A. Mature cancellous bone tissue was observed in the femoral head defect after 2 months implantation. The results indicate that the newly formed injectable bioactive porous grafts could be a good candidate for the treatment of femoral head necrosis.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY, 2019
National Category
Orthopaedics
Identifiers
urn:nbn:se:uu:diva-390518 (URN)10.1039/c9tb00238c (DOI)000472921600011 ()
Available from: 2019-08-14 Created: 2019-08-14 Last updated: 2019-08-14Bibliographically approved
Sladkova, M., Cheng, J., Palmer, M., Chen, S., Lin, C., Xia, W., . . . de Peppo, G. M. (2019). Comparison of Decellularized Cow and Human Bone for Engineering Bone Grafts with Human Induced Pluripotent Stem Cells. Tissue Engineering. Part A, 25(3-4), 288-301
Open this publication in new window or tab >>Comparison of Decellularized Cow and Human Bone for Engineering Bone Grafts with Human Induced Pluripotent Stem Cells
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2019 (English)In: Tissue Engineering. Part A, ISSN 1937-3341, E-ISSN 1937-335X, Vol. 25, no 3-4, p. 288-301Article in journal (Refereed) Published
Abstract [en]

Bone engineering makes it possible to grow unlimited amounts of viable tissue products for basic and applied research, and for clinical applications. A common trend in tissue engineering is the use of decellularized tissue matrices as scaffolding materials, which display structural, mechanical, and biological attributes typical of the native tissue. Due to the limited availability and high cost of human samples, decellularized tissue matrices are typically derived from animal sources. It is unclear, however, whether interspecies differences in tissue parameters will influence the quality of tissue grafts that are engineered using human stem cells. In this study, decellularized cow and human bone scaffolds were compared for engineering bone grafts using human induced pluripotent stem cell-derived mesodermal progenitor cells. After seeding, the cell-scaffold constructs were cultured for 5 weeks in osteogenic medium under dynamic conditions in perfusion bioreactors. The architectural and chemical properties of the scaffolds were studied using microscopic, spectroscopic, and thermogravimetric techniques, while cell behavior and formation of mineralized tissue were assessed using a combination of molecular assays, histological methods, and imaging technologies. The results show that while scaffolds derived from cow and human bone differ somewhat in architecture and composition, both equally support cell viability, tissue growth, and formation of a mineralized bone matrix. Taken together, the results suggest that scaffolds derived from cow bone represent a suitable and convenient alternative to engineer human bone grafts for various biomedical applications.

Keywords
biomaterial scaffold, bone engineering, induced pluripotent stem cells, mesenchymal stem cells, osteogenic differentiation, tissue decellularization
National Category
Biomaterials Science
Identifiers
urn:nbn:se:uu:diva-369891 (URN)10.1089/ten.tea.2018.0149 (DOI)000448565100001 ()30129897 (PubMedID)
Funder
EU, FP7, Seventh Framework Programme
Note

De 2 första författarna delar förstaförfattarskapet.

Available from: 2018-12-19 Created: 2018-12-19 Last updated: 2019-10-25Bibliographically approved
Yang, L., Zhang, M., Xu, L. & Xia, W. (2019). Enhanced luminous efficacy of transparent Ce3+:YAG ceramics with “light-scattering centres” prepared by uniform Ce:Y2O3 submicron spheres for WLEDs. Journal of Luminescence
Open this publication in new window or tab >>Enhanced luminous efficacy of transparent Ce3+:YAG ceramics with “light-scattering centres” prepared by uniform Ce:Y2O3 submicron spheres for WLEDs
2019 (English)In: Journal of Luminescence, ISSN 0022-2313, E-ISSN 1872-7883Article in journal (Refereed) Published
National Category
Ceramics
Identifiers
urn:nbn:se:uu:diva-395273 (URN)
Available from: 2019-10-16 Created: 2019-10-16 Last updated: 2019-10-16
Limaye, M., Schutz, C., Kriechbaum, K., Wohlert, J., Bacsik, Z., Wohlert, M., . . . Bergström, L. (2019). Functionalization and patterning of nanocellulose films by surface-bound nanoparticles of hydrolyzable tannins and multivalent metal ions. Nanoscale
Open this publication in new window or tab >>Functionalization and patterning of nanocellulose films by surface-bound nanoparticles of hydrolyzable tannins and multivalent metal ions
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2019 (English)In: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372Article in journal (Refereed) Published
National Category
Materials Engineering
Identifiers
urn:nbn:se:uu:diva-395276 (URN)
Available from: 2019-10-16 Created: 2019-10-16 Last updated: 2019-10-16
Berg, C., Engqvist, H. & Xia, W. (2019). Ion substitution induced formation of spherical ceramic particles. Ceramics International, 45(8), 10385-10393
Open this publication in new window or tab >>Ion substitution induced formation of spherical ceramic particles
2019 (English)In: Ceramics International, ISSN 0272-8842, E-ISSN 1873-3956, Vol. 45, no 8, p. 10385-10393Article in journal (Refereed) Published
Abstract [en]

How to precipitate ceramic nano- and microspheres in water based solutions only using inorganic ions is a challenge. In this study, spherical particles of alkaline earth phosphates and fluorides were synthesized using a precipitation reaction. Substituting ions, through inhibition of crystal growth, was used to induce sphere formation and to alter the morphology, size and composition of the spheres. The difference in ionic radius between the substituting ion (Mg, Ca and Sr) and the main cation (Sr and Ba) influenced the critical concentration to allow for sphere formation as well as the crystallinity. The larger difference, the lower was the concentration needed to form spheres. Low concentrations of Mg was enough to generate amorphous spheres of Sr- and Ba- phosphates whereas higher concentrations were needed if the radius difference were smaller. An increasing degree of substitution leads to a decrease in crystallinity of precipitated particles. The degree of substitution was determined to 16-55% where a low degree of ion substitution in the phosphates resulted in the formation of spheres (500-800 nm) with rough surfaces composed of apatite like phases. A higher degree of substitution resulted in amorphous spheres (500 nm- (1) mu m) with smooth surfaces.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD, 2019
Keywords
Powders, Chemical preparation, Chemical properties
National Category
Ceramics
Identifiers
urn:nbn:se:uu:diva-383145 (URN)10.1016/j.ceramint.2019.02.097 (DOI)000465058500089 ()
Funder
Swedish Research Council, 2013-5419
Available from: 2019-05-10 Created: 2019-05-10 Last updated: 2019-05-10Bibliographically approved
Hu, Z., Wang, X., Xia, W., Wang, Z., Zhang, P., Xia, L., . . . Zhu, M. (2019). Nano-Structure Designing Promotion Osseointegration of Hydroxyapatite Coated Ti-6Al-4V Alloy Implants in Diabetic Model. Journal of Biomedical Nanotechnology, 15(8), 1701-1713
Open this publication in new window or tab >>Nano-Structure Designing Promotion Osseointegration of Hydroxyapatite Coated Ti-6Al-4V Alloy Implants in Diabetic Model
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2019 (English)In: Journal of Biomedical Nanotechnology, ISSN 1550-7033, E-ISSN 1550-7041, Vol. 15, no 8, p. 1701-1713Article in journal (Refereed) Published
Abstract [en]

Mammalian diabetes mellitus which contains altered microenvironment always impairs diverse cellular processes such as osteogenesis, angiogenesis and tissue regeneration via different mechanisms. For researches in materials science, modifying the ability of osteogenesis and angiogenesis in dental implants shows its significant importance. Nano-structure designing is considered as a facile strategy to improve the surface bioactivity of the implants. In this study, the nanorod-structured hydroxyapatite (HA) coatings on Ti-6Al-4V implants were facilely designed by the combination of plasma-spraying and hydrothermal treatment via varying reaction media. Intriguingly, hydrothermal treatment eliminated the glassy phase and impurity phases of HA coatings, and nanorod-structured surface was successfully constructed under hydrothermal treatment in Na3PO4 solution. Additionally, the HA coatings with nanorod-structured surface effectively promoted the adhesion and proliferation and further enhanced osteogenic differentiation of DM-rBMSCs in vitro, Moreover, the osseointegration of Ti-6Al-4V implants with nanorod-structured HA coating was also enhanced in diabetes mellitus rabbit model in vivo. Therefore, the nano-structured surface modification of HA coating on Ti-6Al-4V implants could target pathological bone loss via strengthening osteogenesis and angiogenesis and further potentially used as a therapeutic coating to promote diabetic osteointegration.

Place, publisher, year, edition, pages
AMER SCIENTIFIC PUBLISHERS, 2019
Keywords
Nano-Structure Designing, Hydroxyapatite, Coating, Osseointegration, Diabetes Mellitus
National Category
Biomaterials Science
Identifiers
urn:nbn:se:uu:diva-390511 (URN)10.1166/jbn.2019.2812 (DOI)000473074400005 ()31219019 (PubMedID)
Available from: 2019-08-12 Created: 2019-08-12 Last updated: 2019-08-12Bibliographically approved
Liu, W., Huan, Z., Xing, M., Tian, T., Xia, W., Wu, C., . . . Chang, J. (2019). Strontium-Substituted Dicalcium Silicate Bone Cements with Enhanced Osteogenesis Potential for Orthopaedic Applications. Materials, 12(14), Article ID 2276.
Open this publication in new window or tab >>Strontium-Substituted Dicalcium Silicate Bone Cements with Enhanced Osteogenesis Potential for Orthopaedic Applications
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2019 (English)In: Materials, ISSN 1996-1944, E-ISSN 1996-1944, Vol. 12, no 14, article id 2276Article in journal (Refereed) Published
Abstract [en]

Incorporating Sr element in biomaterials for bone implants is an effective way to improve their biological performance, as Sr element has been proved to enhance bone regeneration and depress bone resorption activity. In the present study, we developed a Sr-incorporated dicalcium silicate (C2S) bone cement as a potential candidate for bioactive self-setting bone cement in orthopaedics and stomatology. The Sr-C2S powders containing 0.3-6.8% Sr in molar ratio were prepared by means of chemical co-precipitation, and the results of XRD analysis indicated the incorporation of Sr element into the lattice of C2S. Sr-C2S bone cements, as prepared by mixing the powders with water, have a final setting time of 570 to 594 min, and compressive strength higher than that of C2S bone cement within certain incorporation range. The Sr-C2S bone cements possessed good in vitro bioactivity by inducing apatite formation in simulated body fluid (SBF) within 7 days. Moreover, the proliferation activity of human bone marrow mesenchymal stem cells (hBMSCs) with Sr-C2S bone cements was significantly higher than that with C2S bone cement, and the alkaline phosphatase (ALP) activity of hBMSCs was also enhanced with addition of Sr element in Sr-C2S groups. The Sr-C2S might therefore be a bioactive self-setting material with enhanced biological performance and holds the prospect for application in the bone regeneration area.

Place, publisher, year, edition, pages
MDPI, 2019
Keywords
dicalcium silicate, strontium, bone cement, orthopaedics, stomatology, bone regeneration
National Category
Biomaterials Science
Identifiers
urn:nbn:se:uu:diva-393636 (URN)10.3390/ma12142276 (DOI)000480454300064 ()31311147 (PubMedID)
Available from: 2019-09-25 Created: 2019-09-25 Last updated: 2019-09-25Bibliographically approved
Bang, L. T., Filho, L., Engqvist, H., Xia, W. & Persson, C. (2019). Synthesis and assessment of metallic ion migration through a novel calcium carbonate coating for biomedical implants. Journal of Biomedical Materials Research. Part B - Applied biomaterials
Open this publication in new window or tab >>Synthesis and assessment of metallic ion migration through a novel calcium carbonate coating for biomedical implants
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2019 (English)In: Journal of Biomedical Materials Research. Part B - Applied biomaterials, ISSN 1552-4973, E-ISSN 1552-4981Article in journal (Refereed) Published
National Category
Medical Materials
Identifiers
urn:nbn:se:uu:diva-395269 (URN)
Available from: 2019-10-16 Created: 2019-10-16 Last updated: 2019-10-16
Xia, W., Cai, B., Engqvist, H. & Bredenberg, S. (2018). A transdermal drug administration device. JP2018513121A.
Open this publication in new window or tab >>A transdermal drug administration device
2018 (English)Patent (Other (popular science, discussion, etc.))
National Category
Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-372236 (URN)
Patent
JP2018513121A
Available from: 2019-01-07 Created: 2019-01-07 Last updated: 2019-01-07
Yanlun, Z., Lingzhi, K., Fatemeh, F., Xia, W., Chang, J., Yaohua, H. & Haiyan, L. (2018). An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration. Biomaterials, 192, 149-158
Open this publication in new window or tab >>An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration
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2018 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 192, p. 149-158Article in journal (Refereed) Published
Abstract [en]

Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge. In this study, a construct containing an injectable continuous stratified scaffold and multiple cell systems was designed for enhancing osteochondral regeneration. Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration. The continuous SA phase and the stratified structure enable the scaffold to mimic the natural osteochondral structure. In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function. Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed. This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.

National Category
Medical Materials
Identifiers
urn:nbn:se:uu:diva-366108 (URN)10.1016/j.biomaterials.2018.11.017 (DOI)000456902000012 ()30448699 (PubMedID)
Available from: 2018-11-16 Created: 2018-11-16 Last updated: 2019-02-22Bibliographically approved
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Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-7356-3002

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