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Lindahl, Carl
Publications (10 of 27) Show all publications
Lindahl, C., Xia, W., Engqvist, H., Snis, A., Lausmaa, J. & Palmquist, A. (2015). Biomimetic calcium phosphate coating of additively manufactured porous CoCr implants. Applied Surface Science, 353, 40-47
Open this publication in new window or tab >>Biomimetic calcium phosphate coating of additively manufactured porous CoCr implants
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2015 (English)In: Applied Surface Science, ISSN 0169-4332, E-ISSN 1873-5584, Vol. 353, p. 40-47Article in journal (Refereed) Published
Abstract [en]

The aim of this work was to study the feasibility to use a biomimetic method to prepare biomimetic hydroxyapatite (HA) coatings on CoCr substrates with short soaking times and to characterize the properties of such coatings. A second objective was to investigate if the coatings could be applied to porous CoCr implants manufactured by electron beam melting (EBM). The coating was prepared by immersing the pretreated CoCr substrates and EBM implants into the phosphate-buffered solution with Ca2+ in sealed plastic bottles, kept at 60 degrees C for 3 days. The formed coating was partially crystalline, slightly calcium deficient and composed of plate-like crystallites forming roundish flowers in the size range of 300-500 nm. Cross-section imaging showed a thickness of 300-500 nm. In addition, dissolution tests in Tris-HCl up to 28 days showed that a substantial amount of the coating had dissolved, however, undergoing only minor morphological changes. A uniform coating was formed within the porous network of the additive manufactured implants having similar thickness and morphology as for the flat samples. In conclusion, the present coating procedure allows coatings to be formed on CoCr and could be used for complex shaped, porous implants made by additive manufacturing.

National Category
Medical Materials Biomaterials Science
Identifiers
urn:nbn:se:uu:diva-256700 (URN)10.1016/j.apsusc.2015.06.056 (DOI)000361220700006 ()
Available from: 2015-06-25 Created: 2015-06-25 Last updated: 2018-02-08Bibliographically approved
Lindahl, C., Pujari-Palmer, S., Hoess, A., Ott, M., Engqvist, H. & Xia, W. (2015). The influence of Sr content in calcium phosphate coatings. Materials science & engineering. C, biomimetic materials, sensors and systems, 53, 322-330
Open this publication in new window or tab >>The influence of Sr content in calcium phosphate coatings
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2015 (English)In: Materials science & engineering. C, biomimetic materials, sensors and systems, ISSN 0928-4931, E-ISSN 1873-0191, Vol. 53, p. 322-330Article in journal (Refereed) Published
Abstract [en]

In this study calcium phosphate coatings with different amounts of strontium.(Sr) were prepared using a biomineralization method. The incorporation of Sr changed the composition and morphology of coatings from plate-like to sphere-like morphology. Dissolution testing indicated that the solubility of the coatings increased with increased Sr concentration. Evaluation of extracts (with Sr concentrations ranging from 0 to 237 mu g/mL) from the HA, 0.06Sr, 0.6Sr, and 12Sr coatings during in vitro cell cultures showed that Sr incorporation into coatings significantly enhanced the ALP activity in comparison to cells treated with control and HA eluted media. These findings show that calcium phosphate coatings could promote osteogenic differentiation even in a low amount of strontium. (C) 2015 Elsevier B.V. All rights reserved.

Keywords
Strontium, Calcium phosphate coatings, Ion release, In vitro study
National Category
Materials Engineering
Identifiers
urn:nbn:se:uu:diva-260277 (URN)10.1016/j.msec.2015.04.015 (DOI)000358093600039 ()26042720 (PubMedID)
Funder
Swedish Research Council, 2013-5419
Available from: 2015-08-21 Created: 2015-08-18 Last updated: 2018-02-08Bibliographically approved
Thorfve, A., Lindahl, C., Xia, W., Igawa, K., Lindahl, A., Thomsen, P., . . . Tengvall, P. (2014). Hydroxyapatite coating affects the Wnt signaling pathway during peri-implant healing in vivo. Acta Biomaterialia, 10(3), 1451-1462
Open this publication in new window or tab >>Hydroxyapatite coating affects the Wnt signaling pathway during peri-implant healing in vivo
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2014 (English)In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 10, no 3, p. 1451-1462Article in journal (Refereed) Published
Abstract [en]

Owing to its bio- and osteoconductivity, hydroxyapatite (HA) is a widely used implant material, but its osteogenic properties are only partly evaluated in vitro and in vivo. The present study focused on bone healing adjacent to HA-coated titanium (Ti) implants, with or without incorporated lithium ions (Li+). Special attention was given to the Wnt signaling pathway. The implants were inserted into rat tibia for 7 or 28 days and analyzed ex vivo, mainly by histomorphometry and quantitative real-time polymerase chain reaction (qPCR). HA-coated implants showed, irrespective of Li+ content, bone-implant contact (BIC) and removal torque values significantly higher than those of reference Ti. Further, the expression of OCN, CTSK, COL1A1, LRP5/6 and WISP1 was significantly higher in implant-adherent cells of HA-coated implants, with or without Li+. Significantly higher beta-catenin expression and significantly lower COL2A1 expression were observed in pen-implant bone cells from HA with 14 ng cm(-2) released Li+. Interestingly, Ti implants showed a significantly larger bone area (BA) in the threads than HA with 39 ng cm(-2) released Li+, but had a lower BIC than any HA-coated implant. This study shows that HA, with or without Li+, is a strong activator of the Wnt signaling pathway, and may to some degree explain its high bone induction capacity.

National Category
Biomaterials Science Engineering and Technology
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-213615 (URN)10.1016/j.actbio.2013.12.012 (DOI)000331663900039 ()
Funder
Swedish Research Council, K20125-2X-094952-53 6212-0116-037
Available from: 2013-12-30 Created: 2013-12-30 Last updated: 2017-12-06Bibliographically approved
Xia, W., Lindahl, C., Palmqvist, A. & Engqvist, H. (2013). Apatite Coatings: Ion Substitution and Biological Properties. Advanced in Bioceramics and Porous Ceramics
Open this publication in new window or tab >>Apatite Coatings: Ion Substitution and Biological Properties
2013 (English)In: Advanced in Bioceramics and Porous CeramicsArticle in journal (Refereed) Published
National Category
Biomaterials Science Engineering and Technology
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-213621 (URN)
Available from: 2013-12-30 Created: 2013-12-30 Last updated: 2018-02-08
Xia, W., Lindahl, C., Ballo, A., Hoess, A., Pujari, S., Lausmaa, J., . . . Engqvist, H. (2013). Biological properties of ion substituted apatite coatings. In: : . Paper presented at 37th International Conference and Expo on Advanced Ceramics and Composites.
Open this publication in new window or tab >>Biological properties of ion substituted apatite coatings
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2013 (English)Conference paper, Published paper (Refereed)
National Category
Biomaterials Science Engineering and Technology
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-185130 (URN)
Conference
37th International Conference and Expo on Advanced Ceramics and Composites
Available from: 2012-11-20 Created: 2012-11-20 Last updated: 2018-02-08Bibliographically approved
Lindahl, C., Engqvist, H. & Xia, W. (2013). Effect of strontium ions on the early formation of biomimetic apatite on single crystalline rutile. Applied Surface Science, 266, 199-204
Open this publication in new window or tab >>Effect of strontium ions on the early formation of biomimetic apatite on single crystalline rutile
2013 (English)In: Applied Surface Science, ISSN 0169-4332, E-ISSN 1873-5584, Vol. 266, p. 199-204Article in journal (Refereed) Published
Abstract [en]

Single crystalline rutile is a good model to investigate the growth mechanism of hydroxyapatite on bioactive Ti surfaces. Previous studies have shown the difference on different crystalline rutile faces in the early stage and during the growth of HAp crystals from simulated body fluids. It is known that the biological apatite crystal is an ion-substituted apatite. Ion substitution will influence the HAp crystal growth and morphology. In the present study, the effect of strontium ions on the adsorption of Ca and phosphate ions on three different faces of single crystalline rutile substrates has been investigated. The ion adsorption is the crucial step in the nucleation of HAp crystals on specific surfaces. Single crystalline rutile surfaces ((1 1 0), (1 0 0) and (0 0 1)) were soaked in phosphate buffer solutions containing calcium and strontium ions for different time periods. The results showed that the adsorption of Sr, Ca and P is faster on the (1 1 0) surface than on the (1 0 0) and (0 0 1) surfaces. Almost same amount of Sr ion was adsorbed on the surfaces compared to the adsorption of Ca ion. Strontium ion influenced the biological apatite formation in the early stage in this study.

National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-188627 (URN)10.1016/j.apsusc.2012.11.147 (DOI)000314021100032 ()
Note

Correction in: APPLIED SURFACE SCIENCE  Volume: 274   Pages: 426-426

DOI: 10.1016/j.apsusc.2013.02.120

Available from: 2012-12-18 Created: 2012-12-18 Last updated: 2018-02-08Bibliographically approved
Thorfve, A., Lindahl, C., Xia, W., Igawa, K., Emanuelsson, L., Lindahl, A., . . . Tengvall, P. (2013). Hydroxyapatite Coating with Li+ Affects the Wnt Signaling Pathway in vivo. In: : . Paper presented at TERMIS 2013.
Open this publication in new window or tab >>Hydroxyapatite Coating with Li+ Affects the Wnt Signaling Pathway in vivo
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2013 (English)Conference paper, Poster (with or without abstract) (Refereed)
National Category
Biomaterials Science Engineering and Technology
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-213624 (URN)
Conference
TERMIS 2013
Available from: 2013-12-31 Created: 2013-12-31 Last updated: 2018-02-08
Lindahl, C., Engqvist, H. & Xia, W. (2013). Influence of surface treatments on the bioactivity of Ti. Biomaterials, 2013, 205601
Open this publication in new window or tab >>Influence of surface treatments on the bioactivity of Ti
2013 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 2013, p. 205601-Article in journal (Refereed) Published
Abstract [en]

Several techniques have been described to modify the surface of titanium to make it more bioactive. Heat treatment (HT) and sodium hydroxide treatment (NaOH) have been used and can change the crystallinity and surface chemistry of titanium implants. However, no studies have systemically focused on comparing these different methods and their effect on the bioactivity of Ti. Therefore, in this study, Ti substrates were systematically treated using HT, NaOH, and a combination of HT and NaOH. The Ti plates were heat treated at various temperatures, and the plates were subjected to HT followed by soaking in NaOH or first soaked in NaOH and then heat treated. The morphology, crystallinity, hardness, water contact angle, and surface energy of the samples were analyzed as well as the bioactivity after immersion in PBS. Morphology and crystallinity changed with increasing temperature. The difference was most pronounced for the 800°C treated samples. The water contact angle decreased, and the surface energy increased with increasing temperature and was highest for 800°C. The rutile surface showed faster hydroxyapatite formation. NaOH treatment of the HT Ti samples increased the surface energy and improved its bioactivity further. Also, HT of NaOH samples improved the bioactivity compared to only HT.

National Category
Engineering and Technology Bio Materials
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-183342 (URN)10.5402/2013/205601 (DOI)
Available from: 2012-10-24 Created: 2012-10-24 Last updated: 2018-02-08Bibliographically approved
Lilja, M., Lindahl, C., Xia, W., Engqvist, H. & Strømme, M. (2013). The effect of Si-doping on the release of antibiotic from hydroxyapatite coatings. Journal of Biomaterials and Nanobiotechnology, 4(3), 237-241
Open this publication in new window or tab >>The effect of Si-doping on the release of antibiotic from hydroxyapatite coatings
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2013 (English)In: Journal of Biomaterials and Nanobiotechnology, ISSN 2158-7027, E-ISSN 2158-7043, Vol. 4, no 3, p. 237-241Article in journal (Refereed) Published
Abstract [en]

Herein, we show that incorporation of ions during biomimetic coating deposition may be utilized to tailor the drug loading capacity of hydroxyapatite (HA) coatings. Pure biomimetic HA (HA-B) and Si-doped equivalents (SiHA-B) where deposited by a biomimetic process onto titanium dioxide covered titanium substrates. The antibiotic Cephalothin was incorporated into the coatings by adsorptive loading and the release was studied in-vitro. SiHA-B coatings exhibited superior drug incorporation capacity compared to pure HA-B coatings, resulting in a drug release profile dominated by an initial 10 min burst effect while a more prolonged 10 hour release was observed from HA-B coatings. The results emphasize the possibility to impact the drug release kinetics from implant coatings by selective doping elements and the use of thin, biomimetic HA-coatings as drug delivery vehicles. Functionalizing metal implants with SiHA-B coatings presents an interesting strategy towards creating synergetic effects through ion- and antibiotic release and, hence, contributing both towards preventing post-surgical infections while at the same time enhancing the bone-bonding ability.

Keywords
Titanium Dioxide, Hydroxyapatite, Cephalothin, Drug Release, Si-Doping
National Category
Medical Materials
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials; Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-203447 (URN)10.4236/jbnb.2013.43029 (DOI)
Available from: 2013-07-10 Created: 2013-07-10 Last updated: 2018-02-08Bibliographically approved
Lindahl, C., Xia, W. & Engqvist, H. (2012). Apatite coating co-doped with strontium and fluoride using a biomineralization method. Paper presented at Scandinavian Society for Biomaterials (ScSB) Annual Meeting.
Open this publication in new window or tab >>Apatite coating co-doped with strontium and fluoride using a biomineralization method
2012 (English)Conference paper, Published paper (Refereed)
National Category
Biomaterials Science Engineering and Technology
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-185134 (URN)
Conference
Scandinavian Society for Biomaterials (ScSB) Annual Meeting
Available from: 2012-11-20 Created: 2012-11-20 Last updated: 2018-02-08
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