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Alderborn, Anders
Alternative names
Publications (7 of 7) Show all publications
Groebe, K., Cen, J., Schvartz, D., Sargsyan, E., Chowdhury, A. I., Roomp, K., . . . Bergsten, P. (2018). Palmitate-Induced Insulin Hypersecretion and Later Secretory Decline Associated with Changes in Protein Expression Patterns in Human Pancreatic Islets. Journal of Proteome Research, 17(11), 3824-3836
Open this publication in new window or tab >>Palmitate-Induced Insulin Hypersecretion and Later Secretory Decline Associated with Changes in Protein Expression Patterns in Human Pancreatic Islets
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2018 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 17, no 11, p. 3824-3836Article in journal (Refereed) Published
Abstract [en]

In obese children with high circulating concentrations of free fatty acid palmitate, we have observed that insulin levels at fasting and in response to a glucose challenge were several times higher than in obese children with low concentrations of the fatty acid as well as in lean controls. Declining and even insufficient insulin levels were observed in obese adolescents with high levels of the fatty acid. In isolated human islets exposed to palmitate we have observed insulin hypersecretion after 2 days exposure. In contrast, insulin secretion from the islets was reduced after 7 days culture in the presence of the fatty acid. This study aims at identifying islet-related biological events potentially linked with the observed insulin hypersecretion and later secretory decline in these obese children and adolescents using the islet model. We analyzed protein expression data obtained from human islets exposed to elevated palmitate levels for 2 and 7 days by an improved methodology for statistical analysis of differentially expressed proteins. Protein profiling of islet samples by liquid chromatography-tandem mass spectrometry identified 115 differentially expressed proteins (DEPs). Several DEPs including sorcin were associated with increased glucose-stimulated insulin secretion in islets after 2 days of exposure to palmitate. Similarly, several metabolic pathways including altered protein degradation, increased autophagy, altered redox condition, and hampered insulin processing were coupled to the functional impairment of islets after 7 days of culture in the presence of palmitate. Such biological events, once validated in the islets, may give rise to novel treatment strategies aiming at normalizing insulin levels in obese children with high palmitate levels, which may reduce or even prevent obesity-related type 2 diabetes mellitus.

Keywords
childhood obesity, insulin secretion, insulin hypersecretion, insulin hyposecretion, type 2 diabetes mellitus, T2DM, human pancreatic islets, palmitate, in vitro, glucose-stimulated insulin secretion, GSIS, proteomics, differential proteomic analysis, bioinform atic analysis
National Category
Endocrinology and Diabetes Cell and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-369592 (URN)10.1021/acs.jproteome.8b00239 (DOI)000449443000020 ()30183308 (PubMedID)
Funder
EU, FP7, Seventh Framework Programme, 279153
Available from: 2018-12-19 Created: 2018-12-19 Last updated: 2018-12-19Bibliographically approved
Forslund, A., Weghuber, D., Paulmichl, K., Zsoldos, F., Widhalm, K., Vheu, M. D., . . . Bergsten, P. (2017). Exenatide Once Weekly Reduces Weight, Liver Fat And 2-Hour Postprandial Glucose In Obese Adolescents. Acta Paediatrica, 106(470), 14-15
Open this publication in new window or tab >>Exenatide Once Weekly Reduces Weight, Liver Fat And 2-Hour Postprandial Glucose In Obese Adolescents
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2017 (English)In: Acta Paediatrica, ISSN 0803-5253, E-ISSN 1651-2227, Vol. 106, no 470, p. 14-15Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
John Wiley & Sons, 2017
Keywords
Pediatric obesity, reduced glucose tolerance, NAFLD, GLP-1 analog, Exenatide
National Category
Pediatrics
Identifiers
urn:nbn:se:uu:diva-366467 (URN)10.1111/apa.14093 (DOI)000440296300025 ()
Available from: 2018-11-21 Created: 2018-11-21 Last updated: 2018-11-21Bibliographically approved
Nordström, J., Persson, A.-S., Lazorova, L., Frenning, G. & Alderborn, G. (2013). The degree of compression of spherical granular solids controls the evolution of microstructure and bond probability during compaction. International Journal of Pharmaceutics, 442(1-2), 3-12
Open this publication in new window or tab >>The degree of compression of spherical granular solids controls the evolution of microstructure and bond probability during compaction
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2013 (English)In: International Journal of Pharmaceutics, ISSN 0378-5173, E-ISSN 1873-3476, Vol. 442, no 1-2, p. 3-12Article in journal (Refereed) Published
Abstract [en]

The effect of degree of compression on the evolution of tablet microstructure and bond probability during compression of granular solids has been studied. Microcrystalline cellulose pellets of low (about 11%) and of high (about 32%) porosity were used. Tablets were compacted at 50, 100 and 150 MPa applied pressures and the degree of compression and the tensile strength of the tablets determined. The tablets were subjected to mercury intrusion measurements and from the pore size distributions, a void diameter and the porosities of the voids and the intra-granular pores were calculated. The pore size distributions of the tablets had peaks associated with the voids and the intra-granular pores. The void and intra-granular porosities of the tablets were dependent on the original pellet porosity while the total tablet porosity was independent. The separation distance between pellets was generally lower for tablets formed from high porosity pellets and the void size related linearly to the degree of compression. Tensile strength of tablets was higher for tablets of high porosity pellets and a scaled tablet tensile strength related linearly to the degree of compression above a percolation threshold. In conclusion, the degree of compression controlled the separation distance and the probability of forming bonds between pellets in the tablet. 

Keywords
Tablets, Pore structure, Microstructure, Degree of compression, Tensile strength, Percolation theory
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-196531 (URN)10.1016/j.ijpharm.2012.08.011 (DOI)000314690200002 ()
Available from: 2013-03-13 Created: 2013-03-11 Last updated: 2017-12-06Bibliographically approved
Alderborn, A., Sundström, J., Soeria-Atmadja, D., Sandberg, M., Andersson, H. C. & Hammerling, U. (2010). Genetically modified plants for non-food or non-feed purposes: straightforward screening for their appearance in food and feed. Food and Chemical Toxicology, 48(2), 453-464
Open this publication in new window or tab >>Genetically modified plants for non-food or non-feed purposes: straightforward screening for their appearance in food and feed
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2010 (English)In: Food and Chemical Toxicology, ISSN 0278-6915, E-ISSN 1873-6351, Vol. 48, no 2, p. 453-464Article in journal (Refereed) Published
Abstract [en]

Genetically modified (GM) plants aimed at producing food/feed are part of regular agriculture in many areas of the World. Commodity plants have also found application as bioreactors, designated non-food/non-feed GM (NFGM) plants, thereby making raw material for further refinement to industrial, diagnostic or pharmaceutical preparations. Many among them may pose health challenge to consumers or livestock animals, if occurring in food/feed. NFGM plants are typically released into the environment, but are grown under special oversight and any among several containment practices, none of which provide full protection against accidental dispersal. Adventitious admixture with food or feed can occur either through distributional mismanagement or as a consequence of gene flow to plant relatives. To facilitate NFGM surveillance we propose a new mandatory tagging of essentially all such plants, prior to cultivation or marketing in the European Union. The suggested tag--Plant-Made Industrial or Pharmaceutical Products Tag (PMIP-T)--is envisaged to occur as a transgenic silent DNA identifier in host plants and designed to enable technically simple identification and characterisation of any NFGM. Implementation of PMIP-T would permit inexpensive, reliable and high-throughput screening for NFGM specifically. The paper outlines key NFGM prospects and challenges as well as the PMIP-T concept.

National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-119772 (URN)10.1016/j.fct.2009.10.049 (DOI)000275007700001 ()20004226 (PubMedID)
Available from: 2010-03-01 Created: 2010-03-01 Last updated: 2017-12-12Bibliographically approved
Alderborn, A., Kamali, M., Söderberg, O., Schlingemann, H., Nilsson, M. & Landegren, U. (2006). The hunt for cancer biomarkers: Proximity ligation – a new technology for ultra-sensitive protein analysis.
Open this publication in new window or tab >>The hunt for cancer biomarkers: Proximity ligation – a new technology for ultra-sensitive protein analysis
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2006 (Swedish)Other (Other (popular scientific, debate etc.))
Series
BIOforum Europe
Identifiers
urn:nbn:se:uu:diva-10285 (URN)
Available from: 2007-03-12 Created: 2007-03-12
Eriksson, S., Berg, L. M., Wadelius, M. & Alderborn, A. (2002). Cytochrome P450 genotyping by multiplexed real-time DNA sequencing with Pyrosequencing TM technology. Assay and drug development technologies, 1(1), 49-59
Open this publication in new window or tab >>Cytochrome P450 genotyping by multiplexed real-time DNA sequencing with Pyrosequencing TM technology
2002 (English)In: Assay and drug development technologies, ISSN 1540-658X, E-ISSN 1557-8127, Vol. 1, no 1, p. 49-59Article in journal (Refereed) Published
Abstract [en]

Individual differences in xenobiotic metabolism influence the therapeutic value of many drugs and are of major concern during the development of new drug candidates. A number of polymorphic cytochrome p450 enzymes account for a significant part of this variation. A better understanding of these genetic factors would be of value for drug development, as well as clinical practice. To fulfill the goal of a personalized medicine, methods for simple and accurate assessment of cytochrome p450 genes are required. We report on the development of multiplex assays for genotyping of the cytochrome p450 drug-metabolizing enzymes CYP2D6, CYP2C9, and CYP2C19 with Pyrosequencing technology. Eleven variable positions, representing 12 of the most frequent alleles, were scored: CYP2D6 alleles *2, *3, *4, *6, *7, *8, and *14, CYP2C19 alleles *2, *3, and *4, and CYP2C9 alleles *2 and *3. Four multiplex Pyrosequencing reactions per patient sample were performed to cover these positions, using either simplex or multiplex PCR for amplification of target DNA sequences. Unequivocal genotypes were obtained for all patient samples, and the results were validated by comparing with results obtained using PCR-RFLP. For positions addressed with both methods, the results were in complete agreement. Pyrosequencing technology offers a highly automated, rapid, and accurate method for identification of cytochrome p450 alleles, which is suitable for pharmacogenomic research, as well as for routine assessment of patient genotypes.

Keywords
genotyping, pyrosequence, CYP2D6, CYP2C9, CYP2C19, pharmacogenetics
National Category
Pharmacology and Toxicology
Identifiers
urn:nbn:se:uu:diva-64509 (URN)
Available from: 2008-01-18 Created: 2008-01-18 Last updated: 2018-01-10
Andreasson, H., Asp, A., Alderborn, A., Gyllensten, U. & Allen, M. (2002). Mitochondrial sequence analysis for forensic identification using pyrosequencing technology.. Biotechniques, 32, 124-
Open this publication in new window or tab >>Mitochondrial sequence analysis for forensic identification using pyrosequencing technology.
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2002 (English)In: Biotechniques, Vol. 32, p. 124-Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-62801 (URN)
Available from: 2008-10-17 Created: 2008-10-17
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