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Åkerfeldt, Torbjörn
Alternative names
Publications (10 of 15) Show all publications
Rollborn, N., Åkerfeldt, T., Nordin, G., Xu, X. Y., Mandic-Havelka, A., Hansson, L.-O. & Larsson, A. (2017). Analysis of HbA1c on an automated multicapillary zone electrophoresis system.. Scandinavian Journal of Clinical and Laboratory Investigation, 77(1), 15-18
Open this publication in new window or tab >>Analysis of HbA1c on an automated multicapillary zone electrophoresis system.
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2017 (English)In: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, E-ISSN 1502-7686, Vol. 77, no 1, p. 15-18Article in journal (Refereed) Published
Abstract [en]

Hemoglobin A1c (HbA1c) is a frequently requested laboratory test and there is thus a need for high throughput instruments for this assay. We evaluated a new automated multicapillary zone electrophoresis instrument (Capillarys 3 Tera, Sebia, Lisses, France) for analysis of HbA1c in venous samples. Routine requested HbA1c samples were analyzed immunologically on a Roche c6000 instrument (n = 142) and then with the Capillarys 3 Tera instrument. The Capillarys 3 Tera instrument performed approximately 70 HbA1c tests/hour. There was a strong linear correlation between Capillarys 3 Tera and Roche Tina-Quant HbA1c Gen 3 assay (y = 1.003x - 0.3246 R(2 )= .996). The total CV for the 12 capillaries varied between 0.8 and 2.2% and there was a good agreement between duplicate samples (R(2 )= .997). In conclusion, the Capillarys 3 Tera instrument has a high assay capacity for HbA1c. It has a good precision and agreement with the Roche Tina-Quant HbA1c method and is well suited for high volume testing of HbA1c.

National Category
Clinical Laboratory Medicine
Identifiers
urn:nbn:se:uu:diva-305854 (URN)10.1080/00365513.2016.1238507 (DOI)000390847200004 ()27768851 (PubMedID)
Available from: 2016-10-23 Created: 2016-10-23 Last updated: 2017-11-29Bibliographically approved
Brännström, A., Yu, J.-G., Jonsson, P., Åkerfeldt, T., Stridsberg, M. & Svensson, M. (2017). Vitamin D in relation to bone health and muscle function in young female soccer players. European Journal of Sport Science, 17(2), 249-256
Open this publication in new window or tab >>Vitamin D in relation to bone health and muscle function in young female soccer players
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2017 (English)In: European Journal of Sport Science, ISSN 1746-1391, E-ISSN 1536-7290, Vol. 17, no 2, p. 249-256Article in journal (Refereed) Published
Abstract [en]

The present work investigated serum vitamin D (25(OH)D) status in relation to bone and muscle qualities and functions in 19 female soccer players (13-16 years) resident at northern latitude with very low sun exposure (∼32-36 h/month) during winter season (late January to early March). Serum 25(OH)D, parathyroid hormone and bone turnover markers osteocalcin (OC) and beta carboxy-terminal collagen cross-links (β-Ctx), as well as body composition and muscle performance were examined. Hormones were tested using routine laboratory methods. Fat mass, lean mass, and bone mineral density in whole body, as well as femur and lumbar spine were evaluated with dual-energy X-ray absorptiometry. Muscle performance was assessed through isokinetic knee extension and flexion, countermovement jump, and sprint running. 25(OH)D was low (50.5 ±   12.8 nmol l(-1)), whereas the values of bone turnover markers were markedly high (OC: 59.4 ±   18.6 µg l(-1); β-Ctx: 1075 ±   408 ng l(-1)). All bone and muscle measurements were normal or above normal. 25(OH)D was not significantly correlated with most of the parameters of bone and muscle quality or function, except the knee extension time to peak torque (r   =   -0.50, p =   .03). In conclusion, the level of vitamin D is markedly low in adolescent female soccer players during the winter in Sweden. However, vitamin D levels did not significantly correlate with measures of bone and muscle except a moderate correlation in time to peak torque in the knee extensors. The practical implication of low vitamin D levels in young growing female athletes remains unclear.

Keywords
Exercise, education, fitness, health, kinesiology
National Category
Clinical Laboratory Medicine
Identifiers
urn:nbn:se:uu:diva-318361 (URN)10.1080/17461391.2016.1225823 (DOI)000394035100017 ()27633075 (PubMedID)
Available from: 2017-03-24 Created: 2017-03-24 Last updated: 2017-11-29Bibliographically approved
Åkerfeldt, T., Helmersson-Karlqvist, J., Gunningberg, L., Swenne, C. L. & Larsson, A. (2015). Postsurgical Acute Phase Reaction is Associated with Decreased Levels of Circulating Myostatin. Inflammation, 38(4), 1727-1730
Open this publication in new window or tab >>Postsurgical Acute Phase Reaction is Associated with Decreased Levels of Circulating Myostatin
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2015 (English)In: Inflammation, ISSN 0360-3997, E-ISSN 1573-2576, Vol. 38, no 4, p. 1727-1730Article in journal (Refereed) Published
Abstract [en]

Muscle strength is of importance for postsurgical rehabilitation. Myostatin is a growth factor that regulates the size of muscles and could thus influence muscle mass and function in the postsurgical period. The aim of the present study was to study the changes in myostatin levels during the postsurgical inflammatory period. Myostatin was analysed in serum samples from two elective surgery groups, orthopaedic surgery (n = 24) and coronary bypass patients (n = 21). The samples were collected prior to surgery and 4 and 30 days after surgery. In the orthopaedic group, the median myostatin levels decreased from 3582 ng/L prior to surgery to 774 ng/L at day 4 (p < 0.001) and to 2016 ng/L at day 30 (p < 0.001). Median CRP increased from 2.35 mg/L preoperatively to 117 mg/L at day 4 and decreased to 5.5 mg/L at day 30 in the same group. The coronary bypass group showed a similar pattern with a decrease in myostatin from 4212 ng/L to 2574 ng/L at day 4 (p < 0.001) and to 2808 ng/L at day 30 (p = 0.002). Median CRP increased from 1.80 mg/L preoperatively to 136 mg/L at day 4 and returned to 6.12 mg/L at day 30 in the coronary bypass group. There was a significant decrease in myostatin concentrations both in the early and late postsurgical period. The lowest myostatin concentration time point coincided with the highest CRP concentration time point.

National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-253178 (URN)10.1007/s10753-015-0149-6 (DOI)000357666000039 ()25749570 (PubMedID)
Note

Funding: Uppsala University Hospital Research Fund

Available from: 2015-05-23 Created: 2015-05-23 Last updated: 2017-12-04
Åkerfeldt, T., Helmersson-Karlqvist, J., Gordh, T. & Larsson, A. (2014). Circulating Human Fractalkine is Decreased Post-operatively After Orthopedic and Coronary Bypass Surgery. In Vivo, 28(2), 185-188
Open this publication in new window or tab >>Circulating Human Fractalkine is Decreased Post-operatively After Orthopedic and Coronary Bypass Surgery
2014 (English)In: In Vivo, ISSN 0258-851X, E-ISSN 1791-7549, Vol. 28, no 2, p. 185-188Article in journal (Refereed) Published
Abstract [en]

Fractalkine is an important chemokine involved in resolving normal inflammatory processes such as wound healing. Soluble fractalkine acts as a chemoattractant bringing cytotoxic and cytokine-producing cells to areas of inflammation. The aim of the present study was to investigate circulating fractalkine during inflammatory response induced by surgery.

MATERIALS AND METHODS: Fractalkine was analyzed in serum samples from orthopedic surgery patients (n=29) and coronary bypass patients (n=21). The samples were collected prior to surgery and 4 and 30 days after surgery, respectively.

RESULTS: Fractalkine concentrations decreased from pre-operative levels of 1,764 (1,330-2,434) pg/mL to 1,520 (1,330-2,434) pg/mL at 4 days after surgery, and to 1,285 (1,099-1,462) pg/mL 30 days after surgery in patients undergoing orthopedic procedures (p<0.01, 30 days post-operatively versus pre-operatively). Furthermore, fractalkine concentrations decreased significantly from pre-operative levels of 1,856 (1,520-2,434) pg/mL to 1,338 (964-1,650) pg/mL 4 days post-operatively and to 1,266 (1,080-1,338) pg/mL 30 days post-operatively in patients undergoing coronary bypass surgery (p<0.01, 30 days post-operative versus pre-operative values).

CONCLUSION: A significant and persistent decrease in circulating fractalkine was observed after orthopedic and coronary bypass surgery despite a marked inflammatory response.

National Category
Surgery
Identifiers
urn:nbn:se:uu:diva-220706 (URN)000333377600003 ()24632971 (PubMedID)
Available from: 2014-03-19 Created: 2014-03-19 Last updated: 2017-12-05Bibliographically approved
Åkerfeldt, T., Gunningberg, L., Leo Swenne, C., Ronquist, G. & Larsson, A. (2014). Elective orthopedic and cardiopulmonary bypass surgery causes a reduction in serum endostatin levels. European Journal of Medical Research, 19, 61
Open this publication in new window or tab >>Elective orthopedic and cardiopulmonary bypass surgery causes a reduction in serum endostatin levels
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2014 (English)In: European Journal of Medical Research, ISSN 0949-2321, E-ISSN 2047-783X, Vol. 19, p. 61-Article in journal (Refereed) Published
Abstract [en]

Background: Endostatin is an endogenous inhibitor of angiogenesis that inhibits neovascularisation. The aim of the study was to evaluate the effect of elective surgery on endostatin levels.

Methods: Blood samples were collected prior to elective surgery and 4 and 30 days postoperatively in 2 patient groups: orthopedic surgery (n =27) and coronary bypass patients (n =21). Serum endostatin levels were measured by ELISA.

Results: Serum endostatin was significantly reduced 30 days after surgery in comparison with presurgical values in both the orthopedic (P =0.03) and cardiopulmonary surgery (P =0.04) group.

Conclusion: Serum endostatin is reduced 30 days after surgery. This reduction would favor angiogenesis and wound-healing.

National Category
Surgery
Identifiers
urn:nbn:se:uu:diva-235764 (URN)10.1186/s40001-014-0061-9 (DOI)000345944200001 ()25380724 (PubMedID)
Available from: 2014-11-09 Created: 2014-11-09 Last updated: 2017-12-05Bibliographically approved
Ridefelt, P., Åkerfeldt, T. & Helmersson-Karlqvist, J. (2014). Increased plasma glucose levels after change of recommendation from NaF to citrate blood collection tubes. Clinical Biochemistry, 47(7-8), 625-628
Open this publication in new window or tab >>Increased plasma glucose levels after change of recommendation from NaF to citrate blood collection tubes
2014 (English)In: Clinical Biochemistry, ISSN 0009-9120, E-ISSN 1873-2933, Vol. 47, no 7-8, p. 625-628Article in journal (Refereed) Published
Abstract [en]

Objectives: To evaluate changes in plasma glucose measurements in an unselected patient population after a change of recommendation from NaF to citrate blood collection vacuum tubes. Design and methods: Glucose (n = 460 751) and HbA1c (n = 55 190) determinations during a period of approximately three years before and after the tube change were extracted from a laboratory information system. Results: Median values for plasma glucose determinations increased from 6.03 before to 6.28 mmol/L after the tube change. The proportion of glucose determinations above the WHO limit for impaired fasting glucose (6.1 mmol/L) and the medical decision limit for diabetes (7.0 mmol/L) increased from 48.1 to 55.4% after the change. Conclusions: The change from NaF to citrate tubes caused higher glucose values, and consequently more glucose determinations above the decision limit for diabetes.

Keywords
Blood collection tube, Glucose, Plasma, Preanalytical process, Sample stability
National Category
Medical Laboratory and Measurements Technologies
Identifiers
urn:nbn:se:uu:diva-227265 (URN)10.1016/j.clinbiochem.2014.02.022 (DOI)000335905200022 ()
Available from: 2014-07-02 Created: 2014-06-24 Last updated: 2017-12-05Bibliographically approved
Larsson, A., Svensson, M. B., Ronquist, G. & Åkerfeldt, T. (2014). Life style intervention in moderately overweight individuals is associated with decreased levels of cathepsins L and S in plasma. Annals of Clinical and Laboratory Science, 44(3), 283-285
Open this publication in new window or tab >>Life style intervention in moderately overweight individuals is associated with decreased levels of cathepsins L and S in plasma
2014 (English)In: Annals of Clinical and Laboratory Science, ISSN 0091-7370, E-ISSN 1550-8080, Vol. 44, no 3, p. 283-285Article in journal (Refereed) Published
Abstract [en]

Background Adipose tissue cells produce cathepsins L and S, which have proatherogenic effects. Obesity is strongly linked to atherogenesis, cardiovascular morbidity, and mortality.

Objective The aim of the present study was to see if life style interventions/weight reduction could decrease cathepsin L and S levels in blood plasma.

Method Study subjects (n=31) were recruited to a life style intervention program aiming at increased physical activity, more healthy eating habits, and weight reduction for most of the participants. Blood samples were collected at inclusion and after 4 and 8 weeks.

Results Cathepsin L was significantly reduced at 4 weeks (p<0.0001) and 8 weeks (p=0.0004). A similar reduction was also seen for cathepsin S at 4 weeks (p=0.03) and 8 weeks (p=0.008). No significant change in fractalkine values was observed at 4 weeks (p=0.58), but a significant increase was apparent at 8 weeks (p=0.0002).

Conclusion The intervention program resulted in significant reductions of cathepsin L and S levels in plasma after 4 and 8 weeks of intervention.

National Category
Nutrition and Dietetics
Identifiers
urn:nbn:se:uu:diva-228732 (URN)000344755600007 ()25117098 (PubMedID)
Available from: 2014-07-21 Created: 2014-07-21 Last updated: 2017-12-05Bibliographically approved
Åkerfeldt, T., Lipcsey, M., Gunningberg, L., Swenne, C. L. & Larsson, A. (2014). Serum leptin is decreased thirty days after surgery. Journal of Diabetes and Metabolism, 5(12)
Open this publication in new window or tab >>Serum leptin is decreased thirty days after surgery
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2014 (English)In: Journal of Diabetes and Metabolism, ISSN 2155-6156, Vol. 5, no 12Article in journal (Refereed) Published
Abstract [en]

Background: Leptin plays an important role for the regulation of food intake, energy expenditure and glucose control. The aim of this study was to study the effect of surgery on circulating levels of human leptin in a human elective surgery model. Methods: A prospective observational study was conducted. Blood sampling was carried out prior to surgery and four and thirty days after elective surgery, respectively. Patients undergoing orthopedic surgery (n=29) and coronary bypass patients (n=21) were included in the study. Serum leptin levels were measured using sandwich ELISA. C-reactive protein (CRP) was analyzed by turbidimetry. Results: Leptin values was significantly decreased thirty days after surgery in both orthopedic (p=0.002) and coronary bypass patients (p=0.003) in comparison with presurgical values. Conclusion: Elective surgery is associated with decreased leptin levels in the late postsurgical phase.

National Category
Clinical Medicine Endocrinology and Diabetes
Research subject
Medicine
Identifiers
urn:nbn:se:uu:diva-221276 (URN)10.4172/2155-6156.1000465 (DOI)
Available from: 2014-03-27 Created: 2014-03-27 Last updated: 2016-07-20Bibliographically approved
Larsson, A., Ronquist, G. & Åkerfeldt, T. (2013). Lifestyle intervention is associated with decreased concentrations of circulating pentraxin 3 independent of CRP decrease. Upsala Journal of Medical Sciences, 118(3), 165-168
Open this publication in new window or tab >>Lifestyle intervention is associated with decreased concentrations of circulating pentraxin 3 independent of CRP decrease
2013 (English)In: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 118, no 3, p. 165-168Article in journal (Refereed) Published
Abstract [en]

Objectives. Pentraxin 3 (PTX3) is an acute phase marker, which is produced at the site of infection or inflammation in contrast to CRP that is mainly synthesized by the liver. The aim of the present study was to see if lifestyle interventions/weight loss would lead to decreased blood plasma concentrations of PTX3. Methods. Study subjects (n = 31) were recruited to a lifestyle intervention program aiming at increased physical activity, improved eating habits, and weight loss. High-sensitivity C-reactive protein (CRP) and PTX3 methods were used for analysis of CRP and PTX3 in plasma samples collected at inclusion and after 4 and 8 weeks of treatment. Results. Wilcoxon paired samples test showed a significant decrease in PTX3 concentrations from 2068 pg/mL at start to 2007 pg/mL at 4 weeks (P = 0.002) and 1748 pg/mL at 8 weeks (P = 0.003). The PTX3 decrease was not significantly correlated with a corresponding decrease in CRP or weight reduction. Conclusions. The lifestyle intervention program resulted in a significant reduction of circulating concentrations of pentraxin 3 already after 4 and 8 weeks of treatment.

Keywords
Adult, body weight, CRP, human, inflammation, pentraxin 3, plasma
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-204852 (URN)10.3109/03009734.2013.801540 (DOI)000321587100003 ()
Available from: 2013-08-12 Created: 2013-08-12 Last updated: 2017-12-06Bibliographically approved
Larsson, A., Hansson, L.-O. & Åkerfeldt, T. (2013). Weight reduction is associated with decreased CRP levels. Clinical Laboratory, 59(9-10), 1135-1138
Open this publication in new window or tab >>Weight reduction is associated with decreased CRP levels
2013 (English)In: Clinical Laboratory, ISSN 1433-6510, Vol. 59, no 9-10, p. 1135-1138Article in journal (Refereed) Published
Abstract [en]

Background:

Obesity is very costly for society and weight reduction is important to reduce obesity related dis-eases. We have evaluated the effect of weight reduction on CRP values to see if high sensitivity CRP could be used to provide persons on life style intervention programs with positive feedback.

Methods:

Study subjects (n = 26) were recruited to a life style intervention program aiming for weight loss among the laboratory staff at Uppsala University Hospital, Sweden. Blood samples for high sensitivity CRP were collect-ed at inclusion and after 4 weeks. Body composition was estimated by measurements performed on an inexpensive bioimpedance analyzer.

Results:

CRP reduction was significantly associated with weight reduction after four weeks (p = 0.00005) and eight weeks (p = 0.0002). Data from the bioimpedance analyzer were not useful on an individual level.

Conclusions:

High sensitivity CRP could be used to provide positive feedback in workplace weight reduction pro-grams.

National Category
Nutrition and Dietetics
Identifiers
urn:nbn:se:uu:diva-211656 (URN)10.7754/Clin.Lab.2012.121226 (DOI)000328913400024 ()
Available from: 2013-11-27 Created: 2013-11-27 Last updated: 2017-12-06Bibliographically approved
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