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Jalouli, Jamshid
Publications (10 of 13) Show all publications
Jalouli, M. M., Jalouli, J., Öhman, J., Hasseús, B., Hirsch, J. M. & Sand, L. (2015). Association of Human Papilloma Virus infection in healthy oral mucosa, oral dysplasia and oral squamous cell carcinoma. Journal of Oral Health and Dental Management, 14(5)
Open this publication in new window or tab >>Association of Human Papilloma Virus infection in healthy oral mucosa, oral dysplasia and oral squamous cell carcinoma
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2015 (English)In: Journal of Oral Health and Dental Management, ISSN 1583-5588, Vol. 14, no 5Article in journal (Refereed) Published
Abstract [en]

Aims: Human papillomavirus (HPV) is an important risk factor for development of oral cancer; however, the integrational status of the virus into the host DNA association between HPV infection and oral squamous cell carcinoma (OSCC) is still uncertain has not been investigated to the same extent. The objective of the present study was to investigate the prevalence of consensus HPV, and HPV-16 and its integration status, in healthy oral mucosa, oral epithelial dysplasia (OED), and OSCC samples.Materials and Methods: The study material consisted of 26 fresh, normal oral mucosa samples, and 53 and 27 paraffin-embedded OED and OSCC samples, respectively. The specimens were DNA extracted and investigated for the presence of HPV, and HPV-16 and its integration status, by polymerase chain reaction (PCR) and DNA sequencing.Results: Thirty-eight (72%) of the 53 paraffin-embedded OED samples, 16 (59%) of the 27 OSCC samples, and 12 (46%) of the 26 control samples were found to be HPV-DNA positive, with nested PCR (NPCR). Further, HPV-16 was detected in 31 (82%), 15 (94%), and 0 (0%) HPV-positive OED cases, HPV-positive OSCC cases, and controls, respectively. Integration was observed in 26/31 (84%) and 13/15 (87%) of the HPV-16-positive OED and OSCC cases, respectively. A statistically significant difference was found comparing prevalence of HPV-16 in controls with that in both OED and OSCC samples (P<0.005). A statistically significant difference was also found comparing prevalence of integrated and episomal viral forms comparing OED and OSCC samples (P<0.005).Conclusion: The high prevalence of HPV and high-risk HPV-16 in OED and OSCC samples suggests a potential aetiologic role for the virus in OSCC.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-265748 (URN)
Available from: 2015-11-03 Created: 2015-11-03 Last updated: 2017-12-01
Jalouli, M., Jalouli, J., Ibrahim, S. O., Hirsch, J.-M. & Sand, L. (2015). Comparison Between Single PCR and Nested PCR in Detection of Human Papilloma Viruses in Paraffin-embedded OSCC and Fresh Oral Mucosa. In Vivo, 29(1), 65-70
Open this publication in new window or tab >>Comparison Between Single PCR and Nested PCR in Detection of Human Papilloma Viruses in Paraffin-embedded OSCC and Fresh Oral Mucosa
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2015 (English)In: In Vivo, ISSN 0258-851X, E-ISSN 1791-7549, Vol. 29, no 1, p. 65-70Article in journal (Refereed) Published
Abstract [en]

Background: Infection with human papilloma virus (HPV) has been implicated as one of the risk factors for the development of oropharyngeal cancer. Many different HPV tests exist, and information regarding their specific technical, analytical, and clinical properties is increasing. Aim: This study aimed to compare the level of detection of HPV using two reliable polymerase chain reaction (PCR) methods, nested PCR (NPCR) and single PCR (SPCR), in archival paraffin-embedded oral squamous cell carcinoma (OSCC) samples and fresh oral mucosa specimens. Materials and Methods: The presence of HPV genome in two groups of tissue samples was analyzed: (i) 57 paraffin-embedded OSCC samples from Sudan and (ii) eight healthy fresh oral mucosal samples from Swedish volunteers. The specimens were tested by SPCR with primer pair MY9/MY11 and NPCR using GP5+/GP6+ primer sets. Results: Eighteen (32%) out of the 57 paraffin-embedded OSCC samples, and five (62%) out of the eight fresh clinically healthy samples were found to be HPV-positive with NPCR. With SPCR, four (7%) out of the paraffin-embedded OSCC samples were HPV-positive. A statistically significant difference between HPV-positive and -negative samples was found when comparing NPCR and SPCR in OSCC and fresh oral mucosa (p<0.0001). The comparative test between SPCR and NPCR showed 100% sensitivity and 69% specificity for OSCC. Conclusion: The use of the GP5+/GP6+ nested PCR increased the positivity rate, efficiency rate and sensitivity of HPV detection in oral samples significantly and should be considered as the method of choice.

Keywords
HPV, nested PCR, single PCR, paraffin embedded, OSCC, fresh oral mucosa
National Category
Surgery
Identifiers
urn:nbn:se:uu:diva-245198 (URN)000348134300011 ()25600532 (PubMedID)
Available from: 2015-02-25 Created: 2015-02-25 Last updated: 2017-12-04Bibliographically approved
Jalouli, M., Jalouli, J., Hasséus, B., Öhman, J., Hirsch, J. M. & Sand, L. (2015). Nested PCR for detection of HSV-1 in oral mucosa. Medicina Oral, 20(6), E664-E669
Open this publication in new window or tab >>Nested PCR for detection of HSV-1 in oral mucosa
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2015 (English)In: Medicina Oral, ISSN 1698-4447, E-ISSN 1698-6946, Vol. 20, no 6, p. E664-E669Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: It has been estimated that 15%-20% of human tumours are driven by infection and inflammation, and viral infections play an important role in malignant transformation. The evidence that herpes simplex virus type 1 (HSV-1) could be involved in the aetiology of oral cancer varies from weak to persuasive. This study aimed to investigate by nested PCR (NPCR) the prevalence of HSV-1 in samples from normal oral mucosa, oral leukoplakia, and oral squamous cell carcinoma (OSCC).

MATERIAL AND METHODS: We investigated the prevalence of HSV-1 in biopsies obtained from 26 fresh, normal oral mucosa from healthy volunteers as well as 53 oral leukoplakia and 27 OSCC paraffin-embedded samples. DNA was extracted from the specimens and investigated for the presence of HSV-1 by nested polymerase chain reaction (NPCR) and DNA sequencing.

RESULTS: HSV-1 was detected in 14 (54%) of the healthy samples, in 19 (36%) of the oral leukoplakia samples, and in 14 (52%) of the OSCC samples. The differences were not statistically significant.

CONCLUSIONS: We observed a high incidence of HSV-1 in healthy oral mucosa, oral leukoplakia, and OSCC tissues. Thus, no connection between OSCC development and presence of HSV-1 was detected.

Keywords
HSV-1; nested PCR; PCR
National Category
Dentistry
Identifiers
urn:nbn:se:uu:diva-265746 (URN)10.4317/medoral.20630 (DOI)000369435500004 ()26449432 (PubMedID)
Available from: 2015-11-03 Created: 2015-11-03 Last updated: 2017-12-01Bibliographically approved
Sahebjamiee, M., Sand, L., Karimi, S., Biettolahi, J. M., Jabalameli, F. & Jalouli, J. (2015). Prevalence of human papillomavirus in oral lichen planus in an Iranian cohort. Journal of Oral and Maxillofacial Pathology, 19(2), 170-174
Open this publication in new window or tab >>Prevalence of human papillomavirus in oral lichen planus in an Iranian cohort
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2015 (English)In: Journal of Oral and Maxillofacial Pathology, ISSN 0973-029X, E-ISSN 1998-393X, Vol. 19, no 2, p. 170-174Article in journal (Refereed) Published
Abstract [en]

Context: Oral lichen planus (OLP) is a chronic inflammatory mucocutaneous disease with female predominance with the potential for malignant transformation. Human papillomavirus (HPV) is associated with both malignant and benign disease in the head and neck region. Aims: The present study assesses the prevalence of high-risk HPV-16 and HPV-18 in tissue and saliva samples from an Iranian population diagnosed with OLP. Subjects and Methods: Deoxyribonucleic acid was extracted and investigated using polymerase chain reaction from tissue and saliva samples of the same individuals from 40 OLP cases and saliva samples of 40 healthy controls. Statistical Analysis: The prevalence of data was compared using the Chi-square test and inter-group differences were analyzed using Fisher's exact test. Results: OLP specimens were HPV-positive in 11 of 40 (27.5%) cases compared with three of 40 (7.5%) saliva specimens, representing a statistically significant difference (P = 0.0367). HPV-16 and HPV-18 were positive in eight of 40 (20%) OLP tissues and three of 40 (7.5%) saliva samples. Five of the 40 healthy saliva samples were positive for HPV-16. In the OLP patients with dysplasia, four of seven tissue samples were HPV-positive; threeof these four were HPV-16- positive in comparision with seven of 33 HPV-positive samples from OLP patients without dysplasia. Conclusions: Biopsies were more accurate than saliva analysis for evaluating HPV prevalence in OLP patients. HPV prevalence was higher in dysplastic than nondysplastic OLP lesions in this Iranian cohort.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-265747 (URN)DOI: 10.4103/0973-029X.164528 (DOI)
Available from: 2015-11-03 Created: 2015-11-03 Last updated: 2017-12-01
Sand, L., Jalouli, M., Jalouli, J., Ibrahim, S., Hasseus, B., Ohman, J. & Hirsch, J.-M. (2015). Virus and tobacco use in premalignant and malignant oral lesions. Paper presented at 20th World Congress on Advances in Oncology and 18th International Symposium on Molecular Medicine, October 8-10, 2015 Metropolitan Hotel, Athens, Greece. International Journal of Molecular Medicine, 36(Supplement: 1), S27-S27
Open this publication in new window or tab >>Virus and tobacco use in premalignant and malignant oral lesions
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2015 (English)In: International Journal of Molecular Medicine, ISSN 1107-3756, E-ISSN 1791-244X, Vol. 36, no Supplement: 1, p. S27-S27Article in journal, Meeting abstract (Other academic) Published
National Category
Dentistry
Identifiers
urn:nbn:se:uu:diva-266289 (URN)000361863000091 ()
Conference
20th World Congress on Advances in Oncology and 18th International Symposium on Molecular Medicine, October 8-10, 2015 Metropolitan Hotel, Athens, Greece
Available from: 2015-11-06 Created: 2015-11-06 Last updated: 2017-12-01Bibliographically approved
Sand, L. & Jalouli, J. (2014). Viruses and oral cancer. Is there a link?. Microbes and infection, 16(5), 371-378
Open this publication in new window or tab >>Viruses and oral cancer. Is there a link?
2014 (English)In: Microbes and infection, ISSN 1286-4579, E-ISSN 1769-714X, Vol. 16, no 5, p. 371-378Article, review/survey (Refereed) Published
Abstract [en]

Oral squamous cell carcinoma (OSCC) is the most common malignant tumour of the oral cavity. The aetiology of epithelial cancer of the head and neck is considered to be a multifactorial, sequential process. DNA viruses are found in many different cancers and are also capable of transforming cells to a malignant phenotype. Human Papilloma Virus (HPV) has been proposed as risk factors in OSCC development and HPV type 16 is the most important subtype. Other oncogenic virus species i.e., Epstein-Barr Virus and Herpes Simplex Virus Type 1 have been proposed to be involved in oral carcinogenesis. However, no convincing evidence exist that they are an established risk factor in OSCC. Therefore more studies are needed in order to clarify the different aspects of virus involvement. Here, we review the existing literature on viral involvement in oral cancer.

National Category
Surgery Cancer and Oncology
Identifiers
urn:nbn:se:uu:diva-231576 (URN)10.1016/j.micinf.2014.02.009 (DOI)000347866500002 ()24613199 (PubMedID)
Available from: 2014-09-09 Created: 2014-09-09 Last updated: 2017-12-05Bibliographically approved
Jalouli, J., Jalouli, M. M., Sapkota, D., Ibrahim, S. O., Larsson, P.-A. & Sand, L. (2012). Human Papilloma Virus, Herpes Simplex Virus and Epstein Barr Virus in Oral Squamous Cell Carcinoma from Eight Different Countries. Anticancer Research, 32(2), 571-580
Open this publication in new window or tab >>Human Papilloma Virus, Herpes Simplex Virus and Epstein Barr Virus in Oral Squamous Cell Carcinoma from Eight Different Countries
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2012 (English)In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 32, no 2, p. 571-580Article in journal (Refereed) Published
Abstract [en]

Oral squamous cell carcinoma (OSCC) is a major health problem in many parts of the world, and the major causative agents are thought to he the use of alcohol and tobacco. Oncogenic viruses have also been suggested to be involved in OSCC development. This study investigated the prevalence of human papillomaviruses (HPV), herpes simplex virus (HSV) and Epstein-Barr virus (EBV) in 155 OSCC from eight different countries from different ethnic groups, continents and with different socioeconomic backgrounds. 41 A total of OSCCs were diagnosed in the tongue (26%) and 23 in the floor of the mouth (15%); the other 91 OSCCs were diagnosed in other locations (59%). The patients were also investigated regarding the use of alcohol and smoking and smokeless tobacco habits. Tissue samples were obtained from formalin-fixed, paraffin-embedded samples of the OSCC. DNA was extracted and the viral genome was examined by single, nested and seminested PCR assays. Sequencing of double-stranded DNA from the PCR product was carried out. Following sequencing of the HPV-, HSV- and EBV-positive PCR products, 100% homology between the sampels was found. Of all the 155 OSCCs examined, 85 (55%) were positive for EBV, 54 (35%) for HPV and 24 (15%) for HSV. The highest prevalence of HPV was seen in Sudan (65%), while HSV (55%) and EBV (80%) were most prevalent in the UK. In 34% (52/155) of all the samples examined, co-infection by two (46/155=30%) or three (6/155=4%) virus specimens was detected. The most frequent double infection was HPV with EBV in 21% (32/155) of all OSCCs. There was a statistically significant higher proportion of samples with HSV (p=0.026) and EBV (p=0.015) in industrialized countries (Sweden, Norway, UK and USA) as compared to developing countries (Sudan, India, Sri Lanka and Yemen). Furthermore, there was a statistically significant higher co-infection of HSV and EBV in samples from industrialized countries (p=0.00031). No firm conclusions could be drawn regarding the relationship between alcohol, tobacco and virus infections. The significance of our findings must be put in relation to other risk factors and these observations warrant further studies to determine the possible role of viral infections and co-infections with HPV, EBV and HSV as risk markers for the development of OSCC.

Keywords
Epstein Barr virus, herpes simplex virus, human papilloma virus, oral squamous cell carcinoma
National Category
Clinical Medicine
Identifiers
urn:nbn:se:uu:diva-169954 (URN)000299985800023 ()22287747 (PubMedID)
Available from: 2012-03-08 Created: 2012-03-07 Last updated: 2017-12-07Bibliographically approved
Sand, L., Jalouli, M. M., Jalouli, J., Sapkota, D. & Ibrahim, S. O. (2012). p53 Codon 72 polymorphism in oral exfoliated cells in a Sudanese population. In Vivo, 26(1), 59-62
Open this publication in new window or tab >>p53 Codon 72 polymorphism in oral exfoliated cells in a Sudanese population
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2012 (English)In: In Vivo, ISSN 0258-851X, E-ISSN 1791-7549, Vol. 26, no 1, p. 59-62Article in journal (Refereed) Published
Abstract [en]

Earlier studies have investigated the tumor suppressor gene p53 as a co-factor in the development of oral squamous cell carcinoma (OSCC). Our previous studies have indicated that chronic use of Sudanese snuff (toombak) and the presence of human papilloma virus (HPV) may be involved in the high prevalence of OSCC in Sudan. This study investigated the prevalence of p53 codon 72 polymorphism in brush biopsies obtained from a Sudanese population. A total of 174 individuals were included in the study; chronic toombak users (n=152) and non-users (n=22). DNA was extracted from all the samples and genotyped for the codon 72 polymorphism by polymerase chain reaction/restriction fragment length polymorphism. The Arg/Pro genotype was found in 53% of the 174 study participants, compared to 21% found with Arg/Arg and 26% found with Pro/Pro. Stratifying by toombak use, 28 (18%), 45 (29%) and 79 (52%) of the 152 samples from toombak users had Arg/Arg, Pro/Pro and Arg/Pro respectively, compared to 9 (41%), 0 (0%) and 13 (59%) found in the 22 samples from non users. The differences between the samples from toombak users and non users in Arg/Arg and Pro/Pro codon 72 polymorphism and HPV infection were statistically significant (p<0.05). Our study indicated that a high prevalence of the genotype Arg/Pro at the p53 codon 72 may contribute to susceptibility to OSCC, especially in combination with the use of carcinogenic tobacco-specific nitrosamine (TSNA)-rich toombak. Our observations warrant an in-depth study for understanding the role of p53 polymorphism in human oral cancers.

Keywords
p53 polymorphism, oral squamous cell carinoma, p53 codon 72, toombak, Sudan
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-175017 (URN)000298515400007 ()22210716 (PubMedID)
Available from: 2012-05-31 Created: 2012-05-31 Last updated: 2017-12-07Bibliographically approved
Hillbertz, N. S., Hirsch, J.-M., Jalouli, J., Jalouli, M. M. & Sand, L. (2012). Viral and Molecular Aspects of Oral Cancer. Anticancer Research, 32(10), 4201-4212
Open this publication in new window or tab >>Viral and Molecular Aspects of Oral Cancer
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2012 (English)In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 32, no 10, p. 4201-4212Article, review/survey (Refereed) Published
Abstract [en]

Squamous cell carcinoma (SCC) is the most common epithelial malignancy in the oral cavity. SCCs and their variants constitute over 90% of oral malignancies, and the disease is associated with poor prognosis. OSCC is a complex malignancy where environmental factors, virus infections, and genetic alterations most likely interact, and thus give rise to the malignant condition. Herein, we review the available literature regarding high-risk factors such as alcohol and tobacco usage; discuss the roles of human papillomaviruses (HPV), the Epstein-Barr virus, and the human herpes simplex virus (HSV); and evaluate several candidate genes associated with the condition: p53, p16(INK4) and p21(WAF1/CIP1) survivin, B-cell lymphoma-2 (BCL-2), keratins, Fibroblast growth factor 3 (FGF3), FGF4, FGF19, Oral cancer overexpressed gene 1 (ORAOV1), and Cyclin D1 (CCND1).

Keywords
Oral cancer, tobacco, HPV, EBV, HSV-1, p53, p16(INK4A) and p21(WAF1/CIP1), survivin, BCL-2, keratins, FGF3, FGF4, FGF19, ORAOV1, CCND1, review
National Category
Cancer and Oncology Surgery
Identifiers
urn:nbn:se:uu:diva-185673 (URN)000310111800003 ()23060540 (PubMedID)
Available from: 2012-11-27 Created: 2012-11-27 Last updated: 2017-12-07Bibliographically approved
Sand, L., Jalouli, J., Jalouli, M., Ibrahim, S., Larsson, P.-A. -. & Hirsch, J.-M. -. (2012). Virus, genetic alterations and tobacco use in premalignant and malignant oral lesions. International Journal of Molecular Medicine, 30(S1), S9-S9
Open this publication in new window or tab >>Virus, genetic alterations and tobacco use in premalignant and malignant oral lesions
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2012 (English)In: International Journal of Molecular Medicine, ISSN 1107-3756, E-ISSN 1791-244X, Vol. 30, no S1, p. S9-S9Article in journal, Meeting abstract (Other academic) Published
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-188749 (URN)000310651600019 ()
Available from: 2012-12-19 Created: 2012-12-19 Last updated: 2017-12-06Bibliographically approved
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