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Kruys, Åsa
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Publications (10 of 11) Show all publications
Sundberg, H., Ekman, S. & Kruys, Å. (2018). A crush on small fungi: An efficient and quick method forobtaining DNA from minute ascomycetes. Methods in Ecology and Evolution, 9(1), 148-158
Open this publication in new window or tab >>A crush on small fungi: An efficient and quick method forobtaining DNA from minute ascomycetes
2018 (English)In: Methods in Ecology and Evolution, ISSN 2041-210X, E-ISSN 2041-210X, Vol. 9, no 1, p. 148-158Article in journal (Refereed) Published
Abstract [en]

We have developed a reliable technique for extracting DNA from single microscopic fungal thalli, including efficient cell disruption and transfer of cell content for subsequent polymerase chain reaction (PCR). The technique was primarily developed for members of the ascomycete order Laboulbeniales, which are minute fungi with tough cell walls that are exceedingly difficult to disrupt with standard extraction techniques. Our method makes routine amplification of DNA from single thalli possible, even from small species or poorly developed individuals. DNA release is accomplished in an entirely mechanical manner using an arbor press fitted with custom-made components. This approach has eliminated additional treatment such as laborious freeze-thaw cycles, enzymes, or lysing agents. The overall PCR success rate of 89% is comparable to or better than alternative protocols that make use of substantially larger amounts of fungal tissue. From 97% of the successful PCRs a total of 156 sequences from four gene regions were produced. Being able to restrict DNA extractions to a single thallus is critical to all genetic studies requiring data at the level of individual, e.g. population genetics. As all researchers working with minute uncultivable organisms in many respects face the same problems (effective handling of the material, small quantities of DNA etc.), the methodology described here has a potential to be widely applicable. Necessary custom-made components can be manufactured at fairly low cost by any precision-tool workshop using our detail drawings.

National Category
Biological Sciences
Identifiers
urn:nbn:se:uu:diva-334516 (URN)10.1111/2041-210X.12850 (DOI)000419821200015 ()
Funder
Helge Ax:son Johnsons stiftelse
Available from: 2017-11-23 Created: 2017-11-23 Last updated: 2018-02-16Bibliographically approved
Kruys, Å., Huhndorf, S. M. & Miller, A. N. (2015). Coprophilous contributions to the phylogeny of Lasiosphaeriaceae and allied taxa within Sordariales (Ascomycota, Fungi). Fungal diversity, 10(1), 101-113
Open this publication in new window or tab >>Coprophilous contributions to the phylogeny of Lasiosphaeriaceae and allied taxa within Sordariales (Ascomycota, Fungi)
2015 (English)In: Fungal diversity, ISSN 1560-2745, E-ISSN 1878-9129, Vol. 10, no 1, p. 101-113Article in journal (Refereed) Published
Abstract [en]

The phylogenetic relationships of Lasiosphaeriaceae are complicated in that the family is paraphyletic and includes Sordariaceae and Chaetomiaceae, as well as several polyphyletic genera. This study focuses on the phylogenetic relationships of the coprophilous genera, Anopodium, Apodospora, Arnium, Fimetariella and Zygospermella. They are traditionally circumscribed based on ascospore characters, which have proven homoplasious in other genera within the family. Our results based on LSU nrDNA and ß–tubulin sequences distinguish four lineages ofLasiosphaeriaceae taxa. Anopodium joins the clade of morphologically similar, yellow-pigmented species of Cercophora and Lasiosphaeria. Apodospora is monophyletic and joins a larger group of taxa with unclear affinities to each other, while Arnium is polyphyletic being scattered throughout three of the four major clades of Lasiosphaeriaceae. Fimitariella is represented by a single collection and joins the clade containing Cercophora scortea and Podospora appendiculata. Zygospermellashows affinities to the Lasiosphaeris clade. Based on a combination of morphological and molecular data, Echria stat. nov. is recognized at the genus level for the former Arnium section and two new combinations are proposed: E. gigantospora and E. macrotheca.

National Category
Biological Systematics
Identifiers
urn:nbn:se:uu:diva-229949 (URN)10.1007/s13225-014-0296-3 (DOI)000347957000006 ()
Available from: 2014-08-18 Created: 2014-08-18 Last updated: 2017-12-05Bibliographically approved
Kruys, Å. (2015). New species of Preussia with 8-celled ascospores (Sporormiaceae, Pleosporales, Ascomycota). Phytotaxa, 234(2), 143-150
Open this publication in new window or tab >>New species of Preussia with 8-celled ascospores (Sporormiaceae, Pleosporales, Ascomycota)
2015 (English)In: Phytotaxa, ISSN 1179-3155, E-ISSN 1179-3163, Vol. 234, no 2, p. 143-150Article in journal (Refereed) Published
Abstract [en]

The focus of this study is on Preussia sensu lato species with 8-celled ascospores. Two new species, P. alpina and P. octocylindrospora are introduced based on morphological characters and discussed in relation to similar species in the genus. New records are provided from Sporormiella corynespora, S. octomegaspora, P. octomera and P. octonalis. This greatly expands or reduces their geographical distribution ranges, as well as substrate preferences. In addition, a key to the coprophilous species with 8-celled ascospores is provided.

Keywords
Dothideomycetes, Fungi, systematics, taxonomy
National Category
Biological Systematics
Identifiers
urn:nbn:se:uu:diva-274311 (URN)10.11646/phytotaxa.234.2.4 (DOI)000366550600004 ()
Available from: 2016-01-20 Created: 2016-01-20 Last updated: 2017-11-30Bibliographically approved
Kruys, Å. & Castlebury, L. (2012). Molecular phylogeny of Sydowiellaceae: resolving the position of Cainiella. Mycologia, 104(2), 419-426
Open this publication in new window or tab >>Molecular phylogeny of Sydowiellaceae: resolving the position of Cainiella
2012 (English)In: Mycologia, ISSN 0027-5514, E-ISSN 1557-2536, Vol. 104, no 2, p. 419-426Article in journal (Refereed) Published
Abstract [en]

Cainiella is an ascomycete genus associated with arctic alpine plants. The systematic position of Cainiella has long been unclear, with current classifications placing the genus in either Sordariales or Xylariales. Our molecular results, based on mtSSU, ITS and nLSU rDNA data, clearly show that the genus belongs in the Sydowiellaceae (Diaporthales). The study also includes new sequences of Sydowiellaceae and contributes to a better knowledge of the phylogenetic relationships of that family.

Keywords
systematics
National Category
Biological Sciences
Research subject
Biology
Identifiers
urn:nbn:se:uu:diva-171530 (URN)10.3852/11-163 (DOI)000301762100009 ()
Available from: 2012-03-21 Created: 2012-03-20 Last updated: 2017-12-07Bibliographically approved
Kruys, Å. & Eriksson, O. E. (2010). Sporsäckssvampar: svampworkshop med fokus på de allra minsta. Fauna och flora : populär tidskrift för biologi, 105(3), 18-19
Open this publication in new window or tab >>Sporsäckssvampar: svampworkshop med fokus på de allra minsta
2010 (Swedish)In: Fauna och flora : populär tidskrift för biologi, ISSN 0014-8903, Vol. 105, no 3, p. 18-19Article in journal (Other (popular science, discussion, etc.)) Published
Place, publisher, year, edition, pages
Uppsala: SLU Publikationsservice, 2010
National Category
Biological Sciences
Research subject
Biology
Identifiers
urn:nbn:se:uu:diva-142401 (URN)
Available from: 2011-01-13 Created: 2011-01-13 Last updated: 2017-12-11Bibliographically approved
Schoch, C. L., Crous, P. W., Groenewald, J. Z., Boehm, E. W., Burgess, T. I., de Gruyter, J., . . . Spatafora, J. W. (2009). A class-wide phylogenetic assessment of Dothideomycetes. Studies in mycology, 64, 1-15
Open this publication in new window or tab >>A class-wide phylogenetic assessment of Dothideomycetes
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2009 (English)In: Studies in mycology, ISSN 0166-0616, E-ISSN 1872-9797, Vol. 64, p. 1-15Article, review/survey (Refereed) Published
Abstract [en]

We present a comprehensive phylogeny derived from 5 genes, nucSSU, nucLSU rDNA, TEF1, RPB1 and RPB2, for 356 isolates and 41 families (six newly described in this volume) in Dothideomycetes. All currently accepted orders in the class are represented for the first time in addition to numerous previously unplaced lineages. Subclass Pleosporomycetidae is expanded to include the aquatic order Jahnulales. An ancestral reconstruction of basic nutritional modes supports numerous transitions from saprobic life histories to plant associated and lichenised modes and a transition from terrestrial to aquatic habitats are confirmed. Finally, a genomic comparison of 6 dothideomycete genomes with other fungi finds a high level of unique protein associated with the class, supporting its delineation as a separate taxon.

National Category
Biological Sciences
Identifiers
urn:nbn:se:uu:diva-141741 (URN)10.3114/sim.2009.64.01 (DOI)000274365900002 ()20169021 (PubMedID)
Available from: 2011-01-12 Created: 2011-01-12 Last updated: 2017-12-11Bibliographically approved
Kruys, Å. & Wedin, M. (2009). Phylogenetic relationships and an assessment of traditionally used taxonomic characters in the Sporormiaceae (Pleosporales, Dothideomycetes, Ascomycota), utilising multi-gene phylogenies. Systematics and Biodiversity, 7(4), 465-478
Open this publication in new window or tab >>Phylogenetic relationships and an assessment of traditionally used taxonomic characters in the Sporormiaceae (Pleosporales, Dothideomycetes, Ascomycota), utilising multi-gene phylogenies
2009 (English)In: Systematics and Biodiversity, ISSN 1477-2000, E-ISSN 1478-0933, Vol. 7, no 4, p. 465-478Article in journal (Refereed) Published
Abstract [en]

The family Sporormiaceae (Pleosporales, Dothideomycetes, Ascomycota) occur worldwide and a majority of the species are coprophilous. The taxonomy and classification of the family are based on a small number of morphological and ecological characters. Several taxa are easily confused by their shared morphological features, and the relationships between genera are poorly known and in need of critical study. The aims of this study were to resolve the phylogenetic relationships within the Sporormiaceae, test the current generic classification, and study the utility of traditional characters for the taxonomy in the group. To resolve these questions, we analysed combined data sets of ITS-nLSU rDNA, mtSSU rDNA and beta-tubulin sequences with parsimony and Bayesian methods. The results showed that many characters, which previously have been used in the taxonomy and classification of the Sporormiaceae, such as the substrate choice, presence or absence of an ostiole, and presence or absence of germ slits, were all homoplastic and not useful for circumscribing monophyletic groups. A number of characters may be useful for circumscribing smaller clades if used in combination with other features, such as the shape of the ascus with the shape of the ascospores. Our phylogenetic analyses show that Preussia and Sporormiella are non-monophyletic, and a constrained analysis forcing these genera into monophyly resulted in significantly worse trees. Spororminula is nested in Preussia s. lat., and Eremodothis and Pycnidiophora are nested within Westerdykella. Finally, we suggest a new generic classification for the family Sporormiaceae, including Sporormia, Preussia (including Sporomiella and Spororminula) and Westerdykella (including Eremodothis and Pycnidiophora). We also propose 14 new combinations: Preussia alloiomera (S.I. Ahmed & Cain) Kruys, Preussia antarctica (Speg.) Kruys, Preussia bipartis (Cain) Kruys, Preussia borealis (I.Egeland) Kruys, Preussia dubia (S.I. Ahmed & Cain) Kruys, Preussia lignicola (W. Phillips & Plowr.) Kruys, Preussia longisporopsis (S.I. Ahmed & Cain) Kruys, Preussia minipascua (S.I. Ahmed & Cain) Kruys, Preussia octomera (Auersw.) Kruys, Preussia splendens (Cain) Kruys, Preussia tenerifae (Arx & Aa) Kruys, Preussia tetramera (S.I. Ahmed & Cain) Kruys, Westerdykella angulata (A.C. Das) Kruys and Westerdykella aurantiaca (J.N. Rai & J.P. Tewari) Kruys.

Keywords
beta-tubulin, bitunicate, coprophilous, fungi, loculoascomycete, phylogeny, COPROPHILOUS FUNGI, RDNA SEQUENCES, RIBOSOMAL DNA, PCR PRIMERS, LSU RDNA, SSU RDNA, PREUSSIA, GENUS, AMPLIFICATION, SPORORMIELLA
National Category
Biological Sciences
Identifiers
urn:nbn:se:uu:diva-127363 (URN)10.1017/S1477200009990119 (DOI)000272723500011 ()
Available from: 2010-07-13 Created: 2010-07-13 Last updated: 2017-12-12
Kruys, Å. & Ericson, L. (2008). Species richness of coprophilous ascomycetes in relation to variable food intake by herbivores. Fungal diversity, 30, 73-81
Open this publication in new window or tab >>Species richness of coprophilous ascomycetes in relation to variable food intake by herbivores
2008 (English)In: Fungal diversity, ISSN 1560-2745, E-ISSN 1878-9129, Vol. 30, p. 73-81Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to study species richness and species composition of coprophilous ascomycetes in borcal forest. Dung was sampled at three localities and of three herbivores, moose (Alces alces), mountain hare (Lepus timidus), and roe deer (Capreolus capreolus). Species richness differed significantly among dung types and we also found a significant effect of the interaction between locality and dung type on the mean number of species. The highest species richness was found on roe deer dung. While moose did not differ from mountain hare. There was a strong positive relationship between the total number of ascomycete species and the number of plant species foraged by the three herbivores. We believe that food choice is one important factor influencing the species richness of coprophilous ascomycetes, and that some species are more associated with habitat and food choice of the herbivore, rather than a specific dung type/animal species. The composition of species on the different dung types is also discussed. Our results suggests that the coprophilous mycota in the boreal forest is poorly known, we found 47 species in total, four species were undescribed. I was new to Sweden, 24 species were new records for the province, and we made a total of 22 new substrate records.

Keywords
ascomycota, dung inhabiting fungi, fungal ecology, species diversity
National Category
Biological Sciences
Identifiers
urn:nbn:se:uu:diva-161395 (URN)000258548800006 ()
Available from: 2011-11-11 Created: 2011-11-11 Last updated: 2017-12-08Bibliographically approved
Kruys, Å., Eriksson, O. E. & Wedin, M. (2006). Phylogenetic relationships of coprophilous Pleosporales (Dothideomycetes, Ascomycota), and the classification of some bitunicate taxa of unknown position.. Mycological Research, 110, 527-536
Open this publication in new window or tab >>Phylogenetic relationships of coprophilous Pleosporales (Dothideomycetes, Ascomycota), and the classification of some bitunicate taxa of unknown position.
2006 (English)In: Mycological Research, ISSN 0953-7562, E-ISSN 1469-8102, Vol. 110, p. 527-536Article in journal (Refereed) Published
National Category
Natural Sciences
Identifiers
urn:nbn:se:uu:diva-161544 (URN)
Available from: 2011-11-15 Created: 2011-11-15 Last updated: 2017-12-08
Wedin, M., Wiklund, E., Crewe, A., Döring, H., Ekman, S., Nyberg, Å., . . . Lumbsch, T. (2005). Phylogenetic relationships of Lecanoromycetes (Ascomycota) as revealed by analyses of mtSSU and nLSU rDNA sequence data.. Mycological Research, 109, 159-172
Open this publication in new window or tab >>Phylogenetic relationships of Lecanoromycetes (Ascomycota) as revealed by analyses of mtSSU and nLSU rDNA sequence data.
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2005 (English)In: Mycological Research, ISSN 0953-7562, E-ISSN 1469-8102, Vol. 109, p. 159-172Article in journal (Refereed) Published
National Category
Natural Sciences
Identifiers
urn:nbn:se:uu:diva-161546 (URN)
Available from: 2011-11-15 Created: 2011-11-15 Last updated: 2017-12-08
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