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Kisiel, Marta
Alternative names
Publications (10 of 15) Show all publications
Kisiel, M. A., Chmielewski, R. & Bartoszewicz, R. (2019). A rare case of benign teratoma of the facial fronto-temporal region in adult male. Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, 47(280), 150-152
Open this publication in new window or tab >>A rare case of benign teratoma of the facial fronto-temporal region in adult male
2019 (English)In: Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, ISSN 1426-9686, Vol. 47, no 280, p. 150-152Article in journal (Refereed) Published
Abstract [en]

Teratoma is a germ cell neoplasm composed of tissue derived from at least two of three blastodermic embryonic layers (ectoderm, mesoderm and endoderm). The incidence of teratoma in the head and neck region is rare, in particularly in adults.

A CASE REPORT: We reported an uncommon case of teratoma in a 30 years male with a mass in the right fronto-temporal region of the face. Magnetic resonance imaging revealed a well encapsulated heterogenous mass with solid-cystic component extending from the fissure zygomatofrontal and the fissure zygomato-temporal. Serum alfa-fetoprotein levels were not elevated. Surgery was performed, the mass was completely removed, and microscopical analysis confirmed a diagnosis of mature benign teratoma. The patients showed no clinical signs of recurrence in 10 years follow-up. This case of teratoma is extraordinary due to three reasons: the location, the age of patient and benign nature of the tumor in patient at this age. Because such tumors occur exceptionally, each case should be studied with a long-term follow-up and reported.

Keywords
benign teratoma, mature teratoma, teratoma in adult male, teratoma of the facial fronto-temporal region
National Category
Other Health Sciences
Identifiers
urn:nbn:se:uu:diva-401105 (URN)31760398 (PubMedID)
Available from: 2020-01-06 Created: 2020-01-06 Last updated: 2020-01-08Bibliographically approved
Kisiel, M. & Klar, A. S. (2019). Isolation and Culture of Human Dermal Fibroblasts.. Methods in Molecular Biology, 1993, 71-78
Open this publication in new window or tab >>Isolation and Culture of Human Dermal Fibroblasts.
2019 (English)In: Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029, Vol. 1993, p. 71-78Article in journal (Refereed) Published
Abstract [en]

Dermal fibroblasts are the main cell type present in skin connective tissue (dermis). Fibroblasts interact with epidermal cells during hair development and in interfollicular skin. Moreover, they play an essential role during cutaneous wound healing and in bioengineering of skin. Hence, culture of primary fibroblast is gaining in importance. In addition, fibroblasts established from skin biopsies provide a powerful tool for investigating normal skin physiology or specific disease states. In this chapter, detailed procedures for establishing and maintaining primary cultures of adult human dermal fibroblasts are described.

Keywords
Collagen, Dermis, Fibroblasts, Organotypic culture, Regenerative medicine, Skin, Skin equivalent, Tissue engineering
National Category
Occupational Health and Environmental Health
Identifiers
urn:nbn:se:uu:diva-400269 (URN)10.1007/978-1-4939-9473-1_6 (DOI)31148079 (PubMedID)
Available from: 2019-12-19 Created: 2019-12-19 Last updated: 2020-02-06Bibliographically approved
Pierre, P. V., Fransson, A., Kisiel, M. A., Damberg, P., Aski, S. N., Andersson, M., . . . Laurell, G. (2019). Middle Ear Administration of a Particulate Chitosan Gel in an in vivo Model of Cisplatin Ototoxicity. Frontiers in Cellular Neuroscience, 13, Article ID 268.
Open this publication in new window or tab >>Middle Ear Administration of a Particulate Chitosan Gel in an in vivo Model of Cisplatin Ototoxicity
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2019 (English)In: Frontiers in Cellular Neuroscience, ISSN 1662-5102, E-ISSN 1662-5102, Vol. 13, article id 268Article in journal (Refereed) Published
Abstract [en]

Background: Middle ear (intratympanic, IT) administration is a promising therapeutic method as it offers the possibility of achieving high inner ear drug concentrations with low systemic levels, thus minimizing the risk of systemic side effects and drug-drug interactions. Premature elimination through the Eustachian tube may be reduced by stabilizing drug solutions with a hydrogel, but this raises the secondary issue of conductive hearing loss. Aim: This study aimed to investigate the properties of a chitosan-based particulate hydrogel formulation when used as a drug carrier for IT administration in an in vivo model of ototoxicity. Materials and Methods: Two particulate chitosan-based IT delivery systems, Thio-25 and Thio-40, were investigated in albino guinea pigs (n = 94). Both contained the hearing protecting drug candidate sodium thiosulfate with different concentrations of chitosan gel particles (25% vs. 40%). The safety of the two systems was explored in vivo. The most promising system was then tested in guinea pigs subjected to a single intravenous injection with the anticancer drug cisplatin (8 mg/kg b.w.), which has ototoxic side effects. Hearing status was evaluated with acoustically evoked frequency-specific auditory brainstem response (ABR) and hair cell counting. Finally, in vivo magnetic resonance imaging was used to study the distribution and elimination of the chitosan-based system from the middle ear cavity in comparison to a hyaluronan-based system. Results: Both chitosan-based IT delivery systems caused ABR threshold elevations (p < 0.05) that remained after 10 days (p < 0.05) without evidence of hair cell loss, although the elevation induced by Thio-25 was significantly lower than for Thio-40 (p < 0.05). Thio-25 significantly reduced cisplatin-induced ABR threshold elevations (p < 0.05) and outer hair cell loss (p < 0.05). IT injection of the chitosan- and hyaluronan-based systems filled up most of the middle ear space. There were no significant differences between the systems in terms of distribution and elimination. Conclusion: Particulate chitosan is a promising drug carrier for IT administration. Future studies should assess whether the physical properties of this technique allow for a smaller injection volume that would reduce conductive hearing loss.

Place, publisher, year, edition, pages
FRONTIERS MEDIA SA, 2019
Keywords
auditory brainstem response, particulate chitosan, cisplatin, hair cell, hearing loss, intratympanic administration, magnetic resonance imaging, sodium thiosulfate
National Category
Otorhinolaryngology
Identifiers
urn:nbn:se:uu:diva-390821 (URN)10.3389/fncel.2019.00268 (DOI)000473162000001 ()31293387 (PubMedID)
Funder
Vinnova, 2015-00845
Available from: 2019-08-15 Created: 2019-08-15 Last updated: 2019-08-15Bibliographically approved
Fransson, A. E., Kisiel, M., Pirttilä, K., Pettersson, C., Videhult Pierre, P. & Laurell, G. (2017). Hydrogen Inhalation Protects against Ototoxicity Induced by Intravenous Cisplatin in the Guinea Pig. Frontiers in Cellular Neuroscience, 11, Article ID 280.
Open this publication in new window or tab >>Hydrogen Inhalation Protects against Ototoxicity Induced by Intravenous Cisplatin in the Guinea Pig
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2017 (English)In: Frontiers in Cellular Neuroscience, ISSN 1662-5102, E-ISSN 1662-5102, Vol. 11, article id 280Article in journal (Refereed) Published
Abstract [en]

Introduction: Permanent hearing loss and tinnitus as side-effects from treatment with the anticancer drug cisplatin is a clinical problem. Ototoxicity may be reduced by co-administration of an otoprotective agent, but the results in humans have so far been modest.

Aim: The present preclinical in vivo study aimed to explore the protective efficacy of hydrogen (H2) inhalation on ototoxicity induced by intravenous cisplatin.

Materials and Methods: Albino guinea pigs were divided into four groups. The Cispt (n = 11) and Cispt+H2 (n = 11) groups were given intravenous cisplatin (8 mg/kg b.w., injection rate 0.2 ml/min). Immediately after, the Cispt+H2 group also received gaseous H2 (2% in air, 60 min). The H2 group (n = 5) received only H2 and the Control group (n = 7) received neither cisplatin nor H2. Ototoxicity was assessed by measuring frequency specific ABR thresholds before and 96 h after treatment, loss of inner (IHCs) and outer (OHCs) hair cells, and by performing densitometry-based immunohistochemistry analysis of cochlear synaptophysin, organic transporter 2 (OCT2), and copper transporter 1 (CTR1) at 12 and 7 mm from the round window. By utilizing metabolomics analysis of perilymph the change of metabolites in the perilymph was assessed.

Results: Cisplatin induced electrophysiological threshold shifts, hair cell loss, and reduced synaptophysin immunoreactivity in the synapse area around the IHCs and OHCs. H2 inhalation mitigated all these effects. Cisplatin also reduced the OCT2 intensity in the inner and outer pillar cells and in the stria vascularis as well as the CTR1 intensity in the synapse area around the IHCs, the Deiters' cells, and the stria vascularis. H2 prevented the majority of these effects.

Conclusion: H2 inhalation can reduce cisplatin-induced ototoxicity on functional, cellular, and subcellular levels. It is proposed that synaptopathy may serve as a marker for cisplatin ototoxicity. The effect of H2 on the antineoplastic activity of cisplatin needs to be further explored.

Keywords
ABR, inner hair cells, outer hair cells, synaptophysin, organic cation transporter 2, copper transporter 1, perilymph metabolomics, in vivo
National Category
Otorhinolaryngology Analytical Chemistry Pharmaceutical Sciences
Identifiers
urn:nbn:se:uu:diva-330897 (URN)10.3389/fncel.2017.00280 (DOI)000410583900001 ()28955207 (PubMedID)
Available from: 2017-10-06 Created: 2017-10-06 Last updated: 2018-01-13Bibliographically approved
Kisiel, M. (2013). Bone Enhancement with BMP-2 for Safe Clinical Translation. (Doctoral dissertation). Uppsala: Acta Universitatis Upsaliensis
Open this publication in new window or tab >>Bone Enhancement with BMP-2 for Safe Clinical Translation
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Bone morphogenetic protein-2 (BMP-2) is considered a promising adjuvant for the treatment of bone regeneration. However, BMP-2 delivery in a conventional collagen scaffold needs a high dose to achieve an effective outcome. Moreover, such dosage may lead to serious side effects. The aim of the following thesis was to find clinically acceptable strategies reducing the required dose of BMP-2 by improving the delivery and optimizing the preclinical testing of the new approaches. In all the studies hyaluronic acid (HA) hydrogels was used as a carrier for BMP-2.

The HA hydrogel/BMP-2 construct was modified with bioactive matrix components in order to obtain an effective release of BMP-2 and an enhanced bone formation. The most promising were two strategies. In the first one, BMP-2, precomplexed with the glycosaminoglycans dermatan sulfate or heparin prior to loading it into HA hydrogel, protected and prolonged the delivery of the protein, resulting in twofold larger bone formation in comparison to non-complexed BMP-2. In the second strategy, the fibronectin fragment integrin-binding domain (FN) was covalently incorporated into HA hydrogel. The FN remarkably improved the capacity of the material to support the cells attachment and spreading, providing the formation of twice as much bone in comparison to non-functionalized HA hydrogel/BMP-2.

Furthermore, the importance of a proper design of the preclinical study for BMP-2 delivery systems was highlighted. Firstly, proper physicochemical handling of BMP-2 showed the improvement in further in vivo activity.  The use of glass storage vials and an acidic formulation buffer was superior to plastic surfaces and physiological pH. Secondly, while regenerative medicine strategy testing required the use of animal models that matched the research questions related to clinical translation, two new animal models were developed. The subperiosteal mandibular and calvarial models in rats were found to be minimally invasive, convenient and rapid solution for the evaluation of a broad range of approaches including bone augmentation, replacement and regeneration. Both models are primarily relevant for the initial testing of the injectable bone engineering constructs. 

Those clinically translatable approaches presented here could prove to be a powerful platform for a wider use of BMP-2 in orthopedic, plastic surgery and regenerative medicine research.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2013. p. 74
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1009
Keywords
Bone repair, Bone healing, Bone morhogenetic protein-2, Osteogenesis, Extracelular matrix, Hyaluronan, Animal model
National Category
Orthopaedics Biomaterials Science
Research subject
Orthopaedics; Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-188027 (URN)978-91-554-8572-6 (ISBN)
Public defence
2013-02-08, Museum Gustavianum - Auditorium Minus, Akademigatan 3, Uppsala, 13:15 (English)
Opponent
Supervisors
Available from: 2013-01-18 Created: 2012-12-12 Last updated: 2018-01-12Bibliographically approved
Kisiel, M., Klar, A. S., Martino, M. M., Ventura, M. & Hilborn, J. (2013). Evaluation of injectable constructs for bone repair with a subperiosteal cranial model in the rat. PLoS ONE, 8(8), e71683
Open this publication in new window or tab >>Evaluation of injectable constructs for bone repair with a subperiosteal cranial model in the rat
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2013 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, no 8, p. 140p. e71683-Article in journal (Refereed) Published
Abstract [en]

While testing regenerative medicine strategies, the use of animal models that match the research questions and that are related to clinical translation is crucial. During the initial stage of evaluating new strategies for bone repair, the main goal is to state whether the strategies efficiently induce the formation of new bone tissue at an orthotopic site. Here, we present a subperiosteal model in rat calavria that allow the evaluation of a broad range of approaches including bone augmentation, replacement and regeneration. Easy and fast to perform, the model is minimally invasive and no defect are created. The procedure enables to evaluate the outcomes quantitatively using micro-computed tomography and qualitatively by histology and immunohistochemistry. For establishing the model, we used bone morphogenetic protein-2 as an osteoinductive factor and hyaluronic acid hydrogel as injectable biomaterial. We showed that this subperiosteal cranial model offers a minimally invasive and promising solution for a rapid evaluation of bone tissue engineering strategies, even for investigator with limited experience in orthopedic surgery. We believe that this approach could be a powerful platform for orthopedic research and regenerative medicine.

Publisher
p. 140
Keywords
Subperiosteal rat model, Minimally invasive surgery, Regenerative medicine, Bone repairr matrix, Hyaluronan, Animal model
National Category
Biomaterials Science Orthopaedics
Research subject
Orthopaedics; Surgery; Engineering Science; Engineering Science
Identifiers
urn:nbn:se:uu:diva-188001 (URN)10.1371/journal.pone.0071683 (DOI)000323115800075 ()
Available from: 2012-12-13 Created: 2012-12-12 Last updated: 2018-01-12Bibliographically approved
Kisiel, M., Martino, M. M., Ventura, M., Hubbell, J. A., Hilborn, J. & Ossipov, D. A. (2013). Improving the osteogenic potential of BMP-2 with hyaluronic acid hydrogel modified with integrin-specific fibronectin fragment. Biomaterials, 34(3), 704-712
Open this publication in new window or tab >>Improving the osteogenic potential of BMP-2 with hyaluronic acid hydrogel modified with integrin-specific fibronectin fragment
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2013 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 34, no 3, p. 704-712Article in journal (Refereed) Published
Abstract [en]

While human bone morphogenetic protein-2 (rhBMP-2) is a promising growth factor for bone regeneration, its clinical efficacy has recently shown to be below expectation. In order to improve the clinical translation of rhBMP-2, there exists strong motivation to engineer better delivery systems. Hyaluronic acid (HA) hydrogel is a suitable carrier for the delivery of rhBMP-2, but a major limitation of this scaffold is its low cell adhesive properties. In this study, we have determined whether covalent grafting of an integrin-specific ligands into HA hydrogel could improve cell attachment and further enhance the osteogenic potential of rhBMP-2. A structurally stabilized fibronectin (FN) fragment containing the major integrin-binding domain of full-length FN (FN III9 *-10) was engineered, in order to be incorporated into HA hydrogel. Compared to non-functionalized HA hydrogel, HA-FN hydrogel remarkably improved the capacity of the material to support mesenchymal stem cell attachment and spreading. In an ectopic bone formation model in the rat, delivery of rhBMP-2 with HA-FN hydrogel resulted in the formation of twice as much bone with better organization of collagen fibers compared to delivering the growth factor in non-functionalized HA hydrogel. This engineered hydrogel carrier for rhBMP-2 can be relevant in clinical bone repair.

Keywords
Bone regeneration, Cell adhesion, Fibronectin, Hyaluronic acid hydrogel, Integrins, RhBMP-2
National Category
Natural Sciences
Identifiers
urn:nbn:se:uu:diva-184869 (URN)10.1016/j.biomaterials.2012.10.015 (DOI)000312759800011 ()
Available from: 2012-11-21 Created: 2012-11-15 Last updated: 2017-12-07Bibliographically approved
Oommen, O. P., Wang, S., Kisiel, M., Sloff, M., Hilborn, J. & Varghese, O. P. (2013). Smart Design of Stable Extracellular Matrix Mimetic Hydrogel: Synthesis, Characterization, and In Vitro and In Vivo Evaluation for Tissue Engineering. Advanced Functional Materials, 23(10), 1273-1280
Open this publication in new window or tab >>Smart Design of Stable Extracellular Matrix Mimetic Hydrogel: Synthesis, Characterization, and In Vitro and In Vivo Evaluation for Tissue Engineering
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2013 (English)In: Advanced Functional Materials, ISSN 1616-301X, E-ISSN 1616-3028, Vol. 23, no 10, p. 1273-1280Article in journal (Refereed) Published
Abstract [en]

The simplicity and versatility of hydrazone crosslinking has made it a strategy of choice for the conjugation of bioactive molecules. However, the labile nature of hydrazone linkages and reversibility of this coupling reaction restricts its full potential. Based on the fundamental understanding of hydrazone stability, this problem is circumvented by resonance-stabilization of a developing N2 positive charge in a hydrazone bond. A novel chemistry is presented to develop a resilient hydrazone bond that is stable and non- reversible under physiological conditions. A carbodihydrazide (CDH) type hydrazide derivative of the biomolecule forms intrinsically stabilized hydrazone-linkages that are nearly 15-fold more stable at pH 5 than conventional hydrazone. This chemoselective coupling reaction is catalyst-free, instantaneous, and virtually non-cleavable under physiological conditions, therefore can serve as a catalyst-free alternative to click chemistry. This novel crosslinking reaction is used to tailor a hyaluronan hydrogel, which delivered exceptional hydrolytic stability, mechanical properties, low swelling, and controlled enzymatic degradation. These desired characteristics are achieved without increasing the chemical crosslinking. The in vivo evaluation of this hydrogel revealed neo-bone with highly ordered collagen matrix mimicking natural bone regeneration. The proximity ligation assay or PLA is used to detect blood vessels, which highlighted the quality of engineered tissue.

Keywords
biomimetics, biomedical applications, hydrogels, tissue engineering, hyaluronic acid
National Category
Natural Sciences
Identifiers
urn:nbn:se:uu:diva-198387 (URN)10.1002/adfm.201201698 (DOI)000316196100007 ()
Available from: 2013-04-15 Created: 2013-04-15 Last updated: 2017-12-06Bibliographically approved
Nageeb, M., Nouh, S. R., Bergman, K., Nagy, N. B., Khamis, D., Kisiel, M., . . . Marei, M. K. (2012). Bone Engineering by Biomimetic Injectable Hydrogel. Paper presented at 11th International Conference on Frontiers of Polymers and Advanced Materials (ICFPAM 2011). Molecular Crystals and Liquid Crystals, 555(1), 177-188
Open this publication in new window or tab >>Bone Engineering by Biomimetic Injectable Hydrogel
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2012 (English)In: Molecular Crystals and Liquid Crystals, ISSN 1542-1406, Vol. 555, no 1, p. 177-188Article in journal (Refereed) Published
Abstract [en]

Osteoporosis is a multifactorial bone disease characterized by low bone mineral density (BMD) and deterioration of micro-architecture of cancellous bone leading to bone fragility and risk of fractures. In the current work, a novel tissue engineering strategy was experimented to enhance bone architecture in the risk areas via local injection of a biomimetic/osteoinductive injectable hyaluronan based hydrogel loaded with nano-hydroxyapatite crystals (Hya/HA) with/without bone morphogenetic protein (BMP-2), in distal femur of normal and ovariectomized New Zealand white rabbits. Our results revealed the osteoinductive effect of the Hya/HA composite that enhanced bone density and architecture of the rabbit distal femur.

Keywords
Bone engineering, hyaluronic acid, hydroxyapatite, injectable hydrogels, osteoinduction, osteoporosis
National Category
Medical and Health Sciences Polymer Chemistry
Research subject
Chemistry with specialization in Polymer Chemistry
Identifiers
urn:nbn:se:uu:diva-174215 (URN)10.1080/15421406.2012.635530 (DOI)000302300400019 ()
Conference
11th International Conference on Frontiers of Polymers and Advanced Materials (ICFPAM 2011)
Available from: 2012-05-15 Created: 2012-05-14 Last updated: 2013-02-11Bibliographically approved
Kisiel, M., Ventura, M., George, A., Hilborn, J. & Varghese, O. P. (2012). Critical Assessment of rhBMP-2 Mediated Bone Induction: an In Vitro and In Vivo Evaluation. Journal of Tissue Engineering and Regenerative Medicine, 6(SI), 39-39
Open this publication in new window or tab >>Critical Assessment of rhBMP-2 Mediated Bone Induction: an In Vitro and In Vivo Evaluation
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2012 (English)In: Journal of Tissue Engineering and Regenerative Medicine, ISSN 1932-6254, Vol. 6, no SI, p. 39-39Article in journal, Meeting abstract (Other academic) Published
National Category
Natural Sciences Polymer Chemistry
Research subject
Chemistry with specialization in Polymer Chemistry
Identifiers
urn:nbn:se:uu:diva-184592 (URN)000309461100065 ()
Available from: 2012-11-12 Created: 2012-11-09 Last updated: 2013-01-08Bibliographically approved
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