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Berglund, David
Publications (10 of 34) Show all publications
Fuchs, A., Gliwiński, M., Grageda, N., Spiering, R., Abbas, A. K., Appel, S., . . . Trzonkowski, P. (2018). Minimum Information about T Regulatory Cells: A Step toward Reproducibility and Standardization.. Frontiers in Immunology, 8, Article ID 1844.
Open this publication in new window or tab >>Minimum Information about T Regulatory Cells: A Step toward Reproducibility and Standardization.
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2018 (English)In: Frontiers in Immunology, ISSN 1664-3224, E-ISSN 1664-3224, Vol. 8, article id 1844Article in journal (Refereed) Published
Abstract [en]

Cellular therapies with CD4+ T regulatory cells (Tregs) hold promise of efficacious treatment for the variety of autoimmune and allergic diseases as well as posttransplant complications. Nevertheless, current manufacturing of Tregs as a cellular medicinal product varies between different laboratories, which in turn hampers precise comparisons of the results between the studies performed. While the number of clinical trials testing Tregs is already substantial, it seems to be crucial to provide some standardized characteristics of Treg products in order to minimize the problem. We have previously developed reporting guidelines called minimum information about tolerogenic antigen-presenting cells, which allows the comparison between different preparations of tolerance-inducing antigen-presenting cells. Having this experience, here we describe another minimum information about Tregs (MITREG). It is important to note that MITREG does not dictate how investigators should generate or characterize Tregs, but it does require investigators to report their Treg data in a consistent and transparent manner. We hope this will, therefore, be a useful tool facilitating standardized reporting on the manufacturing of Tregs, either for research purposes or for clinical application. This way MITREG might also be an important step toward more standardized and reproducible testing of the Tregs preparations in clinical applications.

Keywords
T regulatory cells, cell therapy, good manufacturing practice, immune tolerance, immunotherapy, minimum information model
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-342893 (URN)10.3389/fimmu.2017.01844 (DOI)000419897500001 ()29379498 (PubMedID)
Funder
EU, Horizon 2020
Available from: 2018-02-23 Created: 2018-02-23 Last updated: 2018-03-01Bibliographically approved
Berglund, E., Sellberg, F. & Berglund, D. (2017). Assessing the purity of regulatory T cells: A humble reminder [Letter to the editor]. Cytotherapy, 19(2), 329-332
Open this publication in new window or tab >>Assessing the purity of regulatory T cells: A humble reminder
2017 (English)In: Cytotherapy, ISSN 1465-3249, E-ISSN 1477-2566, Vol. 19, no 2, p. 329-332Article in journal, Letter (Refereed) Published
Keywords
cell therapy, regulatory T cells, FOXP3, flow cytometry
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-317095 (URN)10.1016/j.jcyt.2016.10.012 (DOI)000392890500017 ()27884702 (PubMedID)
Available from: 2017-03-10 Created: 2017-03-10 Last updated: 2018-01-13Bibliographically approved
Berglund, E., Ljungdahl, M. A., Bogdanovic, D., Berglund, D., Wadstrom, J., Weissenbacher, A.-M., . . . Schneeberger, S. (2017). Clinical Significance Of Alloantibodies In Hand Transplantation - A Multicenter Study. Transplant International, 30, 163-163
Open this publication in new window or tab >>Clinical Significance Of Alloantibodies In Hand Transplantation - A Multicenter Study
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2017 (English)In: Transplant International, ISSN 0934-0874, E-ISSN 1432-2277, Vol. 30, p. 163-163Article in journal, Meeting abstract (Other academic) Published
National Category
Surgery
Identifiers
urn:nbn:se:uu:diva-347297 (URN)000411688501204 ()
Available from: 2018-04-03 Created: 2018-04-03 Last updated: 2018-04-03Bibliographically approved
Berglund, E., Ljungdahl, M. A., Bogdanovic, D., Berglund, D., Wadström, J., Weissenbacher, A., . . . Schneeberger, S. (2017). Clinical Significance of Alloantibodies in Hand Transplantation: Impact on Rejection and Functional Outcome. Paper presented at American Transplant Congress, APR 29-MAY 03, 2017, Chicago, IL. American Journal of Transplantation, 17(S3), 240-240, Article ID 96.
Open this publication in new window or tab >>Clinical Significance of Alloantibodies in Hand Transplantation: Impact on Rejection and Functional Outcome
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2017 (English)In: American Journal of Transplantation, ISSN 1600-6135, E-ISSN 1600-6143, Vol. 17, no S3, p. 240-240, article id 96Article in journal, Meeting abstract (Other academic) Published
National Category
Surgery
Identifiers
urn:nbn:se:uu:diva-335822 (URN)10.1111/ajt.14305 (DOI)000404515702096 ()
Conference
American Transplant Congress, APR 29-MAY 03, 2017, Chicago, IL
Available from: 2018-01-15 Created: 2018-01-15 Last updated: 2018-01-15Bibliographically approved
Strandberg, G., Sellberg, F., Sommar, P., Ronaghi, M., Lubenow, N., Knutson, F. & Berglund, D. (2017). Standardizing the freeze-thaw preparation of growth factors from platelet lysate. Transfusion, 57(4), 1058-1065
Open this publication in new window or tab >>Standardizing the freeze-thaw preparation of growth factors from platelet lysate
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2017 (English)In: Transfusion, ISSN 0041-1132, E-ISSN 1537-2995, Vol. 57, no 4, p. 1058-1065Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Over the past decades, the focus on the regenerative properties of platelets (PLTs) has intensified and many PLT-derived growth factors are readily used in medical settings. A general lack of standardization in the preparation of these growth factors remains, however, and this study therefore examines the dynamics of growth factors throughout the freeze-thaw procedure.

STUDY DESIGN AND METHODS: Plateletpheresis (PA) and PLT-poor plasma (PPP) samples were collected from 10 healthy donors. PA was lysed to produce PLT lysate (PL) for 1, 3, 5, 10, and 30 freeze-thaw cycles. The resulting growth factor and cytokine concentrations from PPP, PA, and PL of different cycles were analyzed and compared using enzyme-linked immunosorbent assay and multiplex bead assays.

RESULTS: PL produced by the freeze-thaw procedure resulted in approximately four-to 10-fold enrichment of transforming growth factor-b1, epidermal growth factor, PLT-derived growth factor (PDGF)-AB/BB, PLT factor-4, and fibroblast growth factor-2. The increase in concentrations plateaued at Cycles 3 and 5 and in some cases declined with further cycles. The concentrations of insulin-like growth factor-1, hepatocyte growth factor, vascular endothelial growth factor, and bone morphogenetic protein-2 in PL were essentially comparable to those in PPP.

CONCLUSION: Using the freeze-thaw method, optimal preparation of PL with regard to the concentration of growth factors was achieved at Cycles 3 to 5. Based on our findings, the clinical significance of using a greater number of cycles is likely limited.

Place, publisher, year, edition, pages
WILEY, 2017
National Category
Hematology
Identifiers
urn:nbn:se:uu:diva-328740 (URN)10.1111/trf.13998 (DOI)000402864500029 ()28182293 (PubMedID)
Available from: 2017-08-30 Created: 2017-08-30 Last updated: 2017-08-30Bibliographically approved
Fredriksson, F., Sellberg, F., Bowden, T., Engstrand, T., Berglund, D. & Engstrand Lilja, H. (2017). Sutures impregnated with carbazate-activated polyvinyl alcohol reduce intraperitoneal adhesions. Journal of Pediatric Surgery, 52(11), 1853-1858
Open this publication in new window or tab >>Sutures impregnated with carbazate-activated polyvinyl alcohol reduce intraperitoneal adhesions
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2017 (English)In: Journal of Pediatric Surgery, ISSN 0022-3468, E-ISSN 1531-5037, Vol. 52, no 11, p. 1853-1858Article in journal (Refereed) Published
Abstract [en]

Background: Intraperitoneal adhesions cause significant morbidity. They occur after peritoneal trauma, which induces oxidative stress with production of inflammatory cytokines, peroxidized proteins (carbonyls) and lipids (aldehydes). This study aimed to investigate if carbazate-activated polyvinyl alcohol (PVAC), an aldehyde-carbonyl inhibitor, can reduce intraperitoneal adhesions in an experimental model.

Material and methods: Male Sprague-Dawley rats (n = 110) underwent laparotomy, cecal abrasion and construction of a small bowel anastomosis. They either were treated with intraperitoneal instillation of PVAC or were sutured with PVAC-impregnated sutures. Thromboelastography analysis was performed using human blood and PVAC. The lipid peroxidation product malondialdehyde (MDA) and inflammatory cytokines IL-1 beta and IL-6 were quantified in peritoneal fluid. At day 7, bursting pressure of the anastomosis was measured and adhesions were blindly scored.

Results: PVAC in human blood decreased the production of the fibrin-thrombocyte mesh without affecting the coagulation cascade. MDA, IL-1 beta and IL-6 were increased after 6 h without significant difference between the groups. PVAC-impregnated sutures reduced intraperitoneal adhesions compared to controls (p = 0.0406) while intraperitoneal instillation of PVAC had no effect. Anastomotic bursting pressure was unchanged.

Conclusions: Intervention with an aldehyde-carbonyl inhibitor locally in the wound by PVAC-impregnated sutures might be a new strategy to reduce intraperitoneal adhesions.

Keywords
Intraperitoneal adhesion prevention, Peroxidation products, Experimental adhesion model
National Category
Surgery
Identifiers
urn:nbn:se:uu:diva-342398 (URN)10.1016/j.jpedsurg.2017.01.058 (DOI)000415328600030 ()28196659 (PubMedID)
Available from: 2018-02-21 Created: 2018-02-21 Last updated: 2018-02-21Bibliographically approved
Sellberg, F., Berglund, E., Ronaghi, M., Strandberg, G., Löf, H., Sommar, P., . . . Berglund, D. (2016). Composition of growth factors and cytokines in lysates obtained from fresh versus stored pathogen-inactivated platelet units. Transfusion and apheresis science, 55(3), 333-337
Open this publication in new window or tab >>Composition of growth factors and cytokines in lysates obtained from fresh versus stored pathogen-inactivated platelet units
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2016 (English)In: Transfusion and apheresis science, ISSN 1473-0502, E-ISSN 1878-1683, Vol. 55, no 3, p. 333-337Article in journal (Refereed) Published
Abstract [en]

Background: Platelet lysate is a readily available source of growth factors, and other mediators, which has been used in a variety of clinical applications. However, the product remains poorly standardized and the present investigation evaluates the composition of platelet lysate obtained from either fresh or stored pathogen-inactivated platelet units.

Materials and Methods: Platelet pooled units (n = 10) were obtained from healthy blood donors and tested according to standard procedures. All units were pathogen inactivated using amotosalen hydrochloride and UVA exposure. Platelet lysate was subsequently produced at two separate time-points, either from fresh platelet units or after 5 days of storage, by repeated freeze-thaw cycles. The following mediators were determined at each time point: EGF, FGF-2, VEGF, IGF-1, PDGF-AB/BB, BMP-2, PF4, TGF-beta isoform 1, IL-1(i, IL-2, IL-6, IL-10, IL-12p70, 1L-17A, TNF-alpha, and IFN-gamma.

Results: The concentration of growth factors and cytokines was affected by time in storage. Notably, TGF-beta, PDGF-AB/BB, and PF4 showed an increase of 27.2% (p < 0.0001), 29.5% (p = 0.04) and 8.2% (p = 0.0004), respectively. A decrease was seen in the levels of IGF-1 and FGF-2 with 22% (p = 0.041) and 11% (p = 0.01), respectively. Cytokines were present only in very low concentrations and all other growth factors remained stable with time in storage.

Conclusion: The composition of mediators in platelet lysate obtained from pathogen inactivated platelet units differs when produced from fresh and stored platelet units, respectively. This underscores the need for further standardization and optimization of this important product, which potentially may influence the clinical effects.

Keywords
Platelet lysate, Pathogen-inactivation, Growth factor, Cytokine
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-314425 (URN)10.1016/j.transci.2016.08.004 (DOI)000390964500014 ()
Available from: 2017-02-02 Created: 2017-02-02 Last updated: 2017-11-29Bibliographically approved
Watanabe, M., Kumagai-Braesch, M., Thunberg, S., Henrikson, J., Sellberg, F., Lundgren, T., . . . Ericzon, B.-G. (2016). Ex vivo generation of alloantigen-specific T regulatory cells using selective T-cell costimulation blockade. Paper presented at International Congress of Immunology (ICI), AUG 21-26, 2016, Melbourne, AUSTRALIA. European Journal of Immunology, 46, 125-125
Open this publication in new window or tab >>Ex vivo generation of alloantigen-specific T regulatory cells using selective T-cell costimulation blockade
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2016 (English)In: European Journal of Immunology, ISSN 0014-2980, E-ISSN 1521-4141, Vol. 46, p. 125-125Article in journal, Meeting abstract (Other academic) Published
National Category
Immunology in the medical area
Identifiers
urn:nbn:se:uu:diva-308494 (URN)000383610400250 ()
Conference
International Congress of Immunology (ICI), AUG 21-26, 2016, Melbourne, AUSTRALIA
Available from: 2016-11-28 Created: 2016-11-28 Last updated: 2018-01-13Bibliographically approved
Sellberg, F., Ronaghi, M., Knutson, F. & Berglund, D. (2016). Inflammatory cytokines in serum samples of healthy blood donors [Letter to the editor]. Transfusion and apheresis science, 55(2), 246-247
Open this publication in new window or tab >>Inflammatory cytokines in serum samples of healthy blood donors
2016 (English)In: Transfusion and apheresis science, ISSN 1473-0502, E-ISSN 1878-1683, Vol. 55, no 2, p. 246-247Article in journal, Letter (Refereed) Published
National Category
Hematology
Identifiers
urn:nbn:se:uu:diva-313620 (URN)10.1016/j.transci.2016.07.020 (DOI)000386744900019 ()27475802 (PubMedID)
Available from: 2017-01-23 Created: 2017-01-23 Last updated: 2017-11-29Bibliographically approved
Massart, A., Pallier, A., Pascual, J., Viklicky, O., Budde, K., Spasovski, G., . . . Abramowicz, D. (2016). The DESCARTES-Nantes survey of kidney transplant recipients displaying clinical operational tolerance identifies 35 new tolerant patients and 34 almost tolerant patients. Nephrology, Dialysis and Transplantation, 31(6), 1002-1013
Open this publication in new window or tab >>The DESCARTES-Nantes survey of kidney transplant recipients displaying clinical operational tolerance identifies 35 new tolerant patients and 34 almost tolerant patients
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2016 (English)In: Nephrology, Dialysis and Transplantation, ISSN 0931-0509, E-ISSN 1460-2385, Vol. 31, no 6, p. 1002-1013Article in journal (Refereed) Published
Abstract [en]

Background. Kidney recipients maintaining a prolonged allograft survival in the absence of immunosuppressive drugs and without evidence of rejection are supposed to be exceptional. The ERA-EDTA-DESCARTES working group together with Nantes University launched a European-wide survey to identify new patients, describe them and estimate their frequency for the first time. Methods. Seventeen coordinators distributed a questionnaire in 256 transplant centres and 28 countries in order to report as many 'operationally tolerant' patients (TOL; defined as having a serum creatinine <1.7 mg/dL and proteinuria <1 g/day or g/g creatinine despite at least 1 year without any immunosuppressive drug) and 'almost tolerant' patients (minimally immunosuppressed patients (MIS) receiving low-dose steroids) as possible. We reported their number and the total number of kidney transplants performed at each centre to calculate their frequency. Results. One hundred and forty-seven questionnaires were returned and we identified 66 TOL (61 with complete data) and 34 MIS patients. Of the 61 TOL patients, 26 were previously described by the Nantes group and 35 new patients are presented here. Most of them were noncompliant patients. At data collection, 31/35 patients were alive and 22/31 still operationally tolerant. For the remaining 9/31, 2 were restarted on immunosuppressive drugs and 7 had rising creatinine of whom 3 resumed dialysis. Considering all patients, 10-year death-censored graft survival post-immunosuppression weaning reached 85% in TOL patients and 100% in MIS patients. With 218 913 kidney recipients surveyed, cumulative incidences of operational tolerance and almost tolerance were estimated at 3 and 1.5 per 10 000 kidney recipients, respectively. Conclusions. In kidney transplantation, operational tolerance and almost tolerance are infrequent findings associated with excellent long-term death-censored graft survival.

Keywords
frequency, graft survival, kidney transplantation, minimally immunosuppressed patients, operational tolerance
National Category
Urology and Nephrology
Identifiers
urn:nbn:se:uu:diva-305952 (URN)10.1093/ndt/gfv437 (DOI)000381213500025 ()26763669 (PubMedID)
Available from: 2016-11-02 Created: 2016-10-24 Last updated: 2017-11-29Bibliographically approved
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