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Hoess, Andreas
Publications (10 of 15) Show all publications
Hoess, A., López, A., Engqvist, H., Ott, M. & Persson, C. (2016). Comparison of a quasi-dynamic and a static extraction method for the cytotoxic evaluation of acrylic bone cements. Materials science & engineering. C, biomimetic materials, sensors and systems, 62, 274-282
Open this publication in new window or tab >>Comparison of a quasi-dynamic and a static extraction method for the cytotoxic evaluation of acrylic bone cements
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2016 (English)In: Materials science & engineering. C, biomimetic materials, sensors and systems, ISSN 0928-4931, E-ISSN 1873-0191, Vol. 62, p. 274-282Article in journal (Refereed) Published
Abstract [en]

In this study, two different extraction approaches were compared in order to evaluate the cytotoxicity of 7 different acrylic bone cements, mainly developed for spinal applications, to osteoblastic cells. Firstly, a static extraction was carried out continuously over 24 h, a method widely used in literature. Secondly, a quasi-dynamic extraction method that allowed the investigation of time-dependent cytotoxic effects of curing acrylic bone cements to cells was introduced. In both cases the extraction of the cements was started at a very early stage of the polymerization process to simulate the conditions during clinical application. Data obtained by the quasi-dynamic extraction method suggest that the cytotoxicity of the setting materials mainly originates from the release of toxic components during the first hour of the polymerization reaction. It was also shown that a static extraction over 24 h generally represents this initial stage of the curing process. Furthermore, compared to the static extraction, time dependent cytotoxicity profiles could be detected using the quasi-dynamic extraction method. Specifically, a modification of commercial Osteopal (R) V with castor oil as a plasticizer as well as a customized cement formulation showed clear differences in cytotoxic behavior compared to the other materials during the setting process. In addition, it was observed that unreacted monomer released from the castor oil modified cement was not the main component affecting the toxicity of the material extracts. The quasi-dynamic extraction method is a useful tool to get deeper insight into the cytotoxic potential of curing acrylic bone cements under relevant biological conditions, allowing systematic optimization of materials under development.

Keywords
Bone cement; PMMA; Cytotoxicity; In vitro; Extraction conditions; Cell culture
National Category
Materials Engineering Medical Materials
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-280836 (URN)10.1016/j.msec.2016.01.048 (DOI)000372759100034 ()
External cooperation:
Funder
VINNOVA, VINNMER 2010-02073
Available from: 2016-03-15 Created: 2016-03-15 Last updated: 2018-02-08Bibliographically approved
Persson, C., López, A., Fathali, H., Hoess, A., Rojas, R., Hilborn, J. & Engqvist, H. (2016). The effect of oligo(trimethylene carbonate) addition on the stiffness of acrylic bone cement. Biomatter, 6(1), Article ID 1133394.
Open this publication in new window or tab >>The effect of oligo(trimethylene carbonate) addition on the stiffness of acrylic bone cement
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2016 (English)In: Biomatter, ISSN 2159-2527, E-ISSN 2159-2535, Vol. 6, no 1, article id 1133394Article in journal (Refereed) Published
Abstract [en]

With the increasing elderly population an increase in the number of bony fractures associated toage-related diseases such as osteoporosis also follows. The relatively high stiffness of the acrylicbone cements used in these patients has been suggested to give raise to a suboptimal loaddistribution surrounding the cementin vivo, and hence contribute to clinical complications, such asadditional fractures. The aim of this study was to develop a low-modulus bone cement, based oncurrently used, commercially available poly(methyl methacrylate) (PMMA) cements forvertebroplasty. To this end, acrylate end-functionalized oligo(trimethylene carbonate) (oTMC) wasincorporated into the cements, and the resulting compressive mechanical properties wereevaluated, as well as the cytotoxic and handling properties of selected formulations. Sixteenwt%oTMC was needed in the vertebroplastic cement Osteopal V to achieve an elastic modulus of1063 MPa (SD 74), which gave a corresponding compressive strength of 46.1 MPa (SD 1.9). Cementextracts taken at 1 and 12 hours gave a reduced MG-63 cell viability in most cases, while extractstaken at 24 hours had no significant effect on cell behavior. The modification also gave an increasein setting time, from 14.7 min (SD 1.7) to 18.0 min (SD 0.9), and a decrease in maximumpolymerization temperature, from 41.5C (SD 3.4) to 30.7C (SD 1.4). While further evaluation ofother relevant properties, such as injectability andin vivobiocompatibility, remains to be done, theresults presented herein are promising in terms of approaching clinically applicable bone cementswith a lower stiffness.

Keywords
bone cement; compression; low-modulus; mechanical properties; PMMA; stiffness; TMC; vertebroplasty
National Category
Other Materials Engineering Medical Materials
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-283476 (URN)10.1080/21592535.2015.1133394 (DOI)
External cooperation:
Funder
EU, FP7, Seventh Framework Programme, 2010-268134VINNOVA, VINNMER 2010-02073
Available from: 2016-04-13 Created: 2016-04-13 Last updated: 2018-02-08Bibliographically approved
Lindahl, C., Pujari-Palmer, S., Hoess, A., Ott, M., Engqvist, H. & Xia, W. (2015). The influence of Sr content in calcium phosphate coatings. Materials science & engineering. C, biomimetic materials, sensors and systems, 53, 322-330
Open this publication in new window or tab >>The influence of Sr content in calcium phosphate coatings
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2015 (English)In: Materials science & engineering. C, biomimetic materials, sensors and systems, ISSN 0928-4931, E-ISSN 1873-0191, Vol. 53, p. 322-330Article in journal (Refereed) Published
Abstract [en]

In this study calcium phosphate coatings with different amounts of strontium.(Sr) were prepared using a biomineralization method. The incorporation of Sr changed the composition and morphology of coatings from plate-like to sphere-like morphology. Dissolution testing indicated that the solubility of the coatings increased with increased Sr concentration. Evaluation of extracts (with Sr concentrations ranging from 0 to 237 mu g/mL) from the HA, 0.06Sr, 0.6Sr, and 12Sr coatings during in vitro cell cultures showed that Sr incorporation into coatings significantly enhanced the ALP activity in comparison to cells treated with control and HA eluted media. These findings show that calcium phosphate coatings could promote osteogenic differentiation even in a low amount of strontium. (C) 2015 Elsevier B.V. All rights reserved.

Keywords
Strontium, Calcium phosphate coatings, Ion release, In vitro study
National Category
Materials Engineering
Identifiers
urn:nbn:se:uu:diva-260277 (URN)10.1016/j.msec.2015.04.015 (DOI)000358093600039 ()26042720 (PubMedID)
Funder
Swedish Research Council, 2013-5419
Available from: 2015-08-21 Created: 2015-08-18 Last updated: 2018-02-08Bibliographically approved
Pujari, S., Hoess, A., Shen, J., Thormann, A., Heilmann, A., Tang, L. & Karlsson-Ott, M. (2014). Effect of nanoporosity on inflammatory cells. European Journal of Clinical Investigation, 44(S1), 36-36
Open this publication in new window or tab >>Effect of nanoporosity on inflammatory cells
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2014 (English)In: European Journal of Clinical Investigation, ISSN 0014-2972, E-ISSN 1365-2362, Vol. 44, no S1, p. 36-36Article in journal, Meeting abstract (Other academic) Published
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-224767 (URN)000334080900101 ()
Available from: 2014-05-21 Created: 2014-05-20 Last updated: 2017-12-05Bibliographically approved
Pujari, S., Hoess, A., Shen, J., Thormann, A., Heilmann, A., Tang, L. & Karlsson-Ott, M. (2014). Effects of nanoporous alumina on inflammatory cell response. Journal of Biomedical Materials Research. Part A, 102(11), 3773-3780
Open this publication in new window or tab >>Effects of nanoporous alumina on inflammatory cell response
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2014 (English)In: Journal of Biomedical Materials Research. Part A, ISSN 1549-3296, E-ISSN 1552-4965, Vol. 102, no 11, p. 3773-3780Article in journal (Refereed) Published
Abstract [en]

The present study focuses on the effects of nanoscale porosity on inflammatory response in vitro and in vivo. Nanoporous alumina membranes with different pore sizes, 20 and 200 nm in diameter, were used. We first evaluated cell/alumina interactions in vitro by observing adhesion, proliferation, and activation of a murine fibroblast and a macrophage cell line. To investigate the chronic inflammatory response, the membranes were implanted subcutaneously in mice for 2 weeks. Cell recruitment to the site of implantation was determined by histology and the production of cytokines was measured by protein array analysis. Both in vitro and in vivo studies showed that 200 nm pores induced a stronger inflammatory response as compared to the alumina with 20 nm pores. This was observed by an increase in macrophage activation in vitro as well as higher cell recruitment and generation of proinflammatory cytokines around the alumina with 200 nm pores, in vivo. Our results suggest that nanofeatures can be modulated in order to control the inflammatory response to implants.

Keywords
nanoporous alumina, nanofeatures, inflammation, in vivo
National Category
Biomaterials Science Engineering and Technology
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-236525 (URN)10.1002/jbm.a.35048 (DOI)000343010100001 ()24288233 (PubMedID)
Available from: 2014-12-01 Created: 2014-11-19 Last updated: 2017-12-05Bibliographically approved
Xia, W., Lindahl, C., Ballo, A., Hoess, A., Pujari, S., Lausmaa, J., . . . Engqvist, H. (2013). Biological properties of ion substituted apatite coatings. In: : . Paper presented at 37th International Conference and Expo on Advanced Ceramics and Composites.
Open this publication in new window or tab >>Biological properties of ion substituted apatite coatings
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2013 (English)Conference paper, Published paper (Refereed)
National Category
Biomaterials Science Engineering and Technology
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-185130 (URN)
Conference
37th International Conference and Expo on Advanced Ceramics and Composites
Available from: 2012-11-20 Created: 2012-11-20 Last updated: 2018-02-08Bibliographically approved
López, A., Hoess, A., Fathali, H., Ott, M., Engqvist, H. & Persson, C. (2012). Acrylic bone cement modified with oligo (trimethylene carbonate). In: 5th Annual Meeting of the Scandinavian Society for Biomaterials, Uppsala Sweden, May 8-9, 2012. Paper presented at 5th Annual Meeting of the Scandinavian Society for Biomaterials, Uppsala Sweden, May 8-9, 2012.
Open this publication in new window or tab >>Acrylic bone cement modified with oligo (trimethylene carbonate)
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2012 (English)In: 5th Annual Meeting of the Scandinavian Society for Biomaterials, Uppsala Sweden, May 8-9, 2012, 2012Conference paper, Poster (with or without abstract) (Refereed)
Series
European Cells and Materials, ISSN 1473-2262
Keywords
PMMA, bone cement(s), low modulus, oligo(trimethylene carbonate)
National Category
Medical Materials
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-174548 (URN)
Conference
5th Annual Meeting of the Scandinavian Society for Biomaterials, Uppsala Sweden, May 8-9, 2012
Available from: 2012-05-29 Created: 2012-05-22 Last updated: 2018-02-08Bibliographically approved
Xia, W., Grandfield, K., Hoess, A., Ballo, A., Cai, Y. & Engqvist, H. (2012). Mesoporous titanium dioxide coating for metallic implants. Journal of Biomedical Materials Research. Part B - Applied biomaterials, 100B(1), 82-93
Open this publication in new window or tab >>Mesoporous titanium dioxide coating for metallic implants
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2012 (English)In: Journal of Biomedical Materials Research. Part B - Applied biomaterials, ISSN 1552-4973, E-ISSN 1552-4981, Vol. 100B, no 1, p. 82-93Article in journal (Refereed) Published
Abstract [en]

A bioactive mesoporous titanium dioxide (MT) coating for surface drug delivery has been investigated to develop a multifunctional implant coating, offering quick bone bonding and biological stability. An evaporation induced self-assembly (EISA) method was used to prepare a mesoporous titanium dioxide coating of the anatase phase with BET surface area of 172 m2/g and average pore diameter of 4.3 nm. Adhesion tests using the scratch method and an in situ screw-in/screw-out technique confirm that the MT coating bonds tightly with the metallic substrate, even after removal from bone. Because of its high surface area, the bioactivity of the MT coating is much better than that of a dense TiO2 coating of the same composition. Quick formation of hydroxyapatite (HA) in vitro can be related to enhance bonding with bone. The uptake of antibiotics by the MT coating reached 13.4 mg/cm3 within a 24 h loading process. A sustained release behavior has been obtained with a weak initial burst. By using Cephalothin as a model drug, drug loaded MT coating exhibits a sufficient antibacterial effect on the material surface, and within millimeters from material surface, against E.coli. Additionally, the coated and drug loaded surfaces showed no cytotoxic effect on cell cultures of the osteoblastic cell line MG-63. In conclusion, this study describes a novel, biocompatiblemesoporous implant coating, which has the ability to induce HA formation and could be used as a surface drug-delivery system.

Keywords
mesoporous materials, titanium oxide, drug delivery, bioactivity, implant coating
National Category
Biomaterials Science Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials; Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-159881 (URN)10.1002/jbm.b.31925 (DOI)000297949800010 ()
Available from: 2011-10-11 Created: 2011-10-11 Last updated: 2018-02-08Bibliographically approved
Hoess, A., Thormann, A., Friedmann, A. & Heilmann, A. (2012). Self-supporting nanoporous alumina membranes as substrates for hepatic cell cultures. Journal of Biomedical Materials Research. Part A, 100A(9), 2230-2238
Open this publication in new window or tab >>Self-supporting nanoporous alumina membranes as substrates for hepatic cell cultures
2012 (English)In: Journal of Biomedical Materials Research. Part A, ISSN 1549-3296, E-ISSN 1552-4965, Vol. 100A, no 9, p. 2230-2238Article in journal (Refereed) Published
Abstract [en]

Membranes made from nanoporous alumina exhibit interesting properties for their use in biomedical research. They show high porosity and the pore diameters can be easily adjusted in a reproducible manner. Nanoporous alumina membranes are thus ideal substrates for the cultivation of polar cells (e.g., hepatocytes) or the establishment of indirect co-cultures. The porous nature of the material allows supply of nutrients to both sides of adherent cells and the exchange of molecules across the membrane. However, it is well-known that surface features in the nanometer range affect cellular behavior. In this study, the response of HepG2 cells to nanoporous alumina membranes with three different pore diameters, ranging from 50 to 250 nm, has been evaluated. The cellular interactions with the nanoporous materials were assessed by investigating cell adhesion, morphology, and proliferation. Cell functionality was measured by means of albumin production. The membranes supported good cell adhesion and spreading. Compared to tissue culture plastic, the cells on the porous substrates developed distinct focal adhesion sites and actin stress fibers. Additionally, electron microscopical investigations revealed the penetration of cellular extensions into pores with diameters bigger than 200 nm. Furthermore, cell proliferation significantly increased with an increase in pore diameter, whereas the albumin production followed a reverse trend. Thus, it seems to be possible to direct cellular behavior of HepG2 cells growing on nanoporous alumina by changing the pore diameter of the material. Hence, nanoporous alumina membranes can be useful culture substrates to develop new approaches in the field of liver tissue engineering.

Keywords
nanoporous alumina, HepG2, cell culture, cell adhesion, scanning electron microscopy
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-180269 (URN)10.1002/jbm.a.34158 (DOI)000306922000003 ()
Available from: 2012-09-03 Created: 2012-09-03 Last updated: 2017-12-07Bibliographically approved
Forsgren, J., Hoess, A., Ott, M., Maria, S. & Engqvist, H. (2011). A Soluble Strontium Carbonate Implant Coating for Local and Targeted Cell Stimulation. In: MRS Spring Meeeting 2011: . Paper presented at MRS spring meeting 2011.
Open this publication in new window or tab >>A Soluble Strontium Carbonate Implant Coating for Local and Targeted Cell Stimulation
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2011 (English)In: MRS Spring Meeeting 2011, 2011Conference paper, Published paper (Refereed)
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Materials Science; Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-146064 (URN)
Conference
MRS spring meeting 2011
Available from: 2011-02-15 Created: 2011-02-15 Last updated: 2018-02-08Bibliographically approved
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