uu.seUppsala University Publications
Change search
Link to record
Permanent link

Direct link
BETA
Karawajczyk, Malgorzata
Publications (9 of 9) Show all publications
Wallin, K.-L., Botero-Kleiven, S., Blackberg, J., Persson, K., Schmidt, B., Ogren, J., . . . Bengtsson, H.-I. (2018). Evaluation Of A Novel Prototype Software For Image Analysis Of Malaria Parasites. International Journal of Laboratory Hematology, 40(S2), 155-155
Open this publication in new window or tab >>Evaluation Of A Novel Prototype Software For Image Analysis Of Malaria Parasites
Show others...
2018 (English)In: International Journal of Laboratory Hematology, ISSN 1751-5521, E-ISSN 1751-553X, Vol. 40, no S2, p. 155-155Article in journal, Meeting abstract (Other academic) Published
National Category
Hematology
Identifiers
urn:nbn:se:uu:diva-366389 (URN)000444677800284 ()
Available from: 2018-11-21 Created: 2018-11-21 Last updated: 2018-11-21Bibliographically approved
Hallberg, P., Nagy, J., Karawajczyk, M., Nordang, L., Islander, G., Norling, P., . . . Wadelius, M. (2017). Comparison of Clinical Factors Between Patients With Angiotensin-Converting Enzyme Inhibitor-Induced Angioedema and Cough. The Annals of Pharmacotherapy, 51(4), 293-300
Open this publication in new window or tab >>Comparison of Clinical Factors Between Patients With Angiotensin-Converting Enzyme Inhibitor-Induced Angioedema and Cough
Show others...
2017 (English)In: The Annals of Pharmacotherapy, ISSN 1060-0280, E-ISSN 1542-6270, Vol. 51, no 4, p. 293-300Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Angioedema is a rare and serious adverse drug reaction (ADR) to angiotensin-converting enzyme (ACE) inhibitor treatment. Dry cough is a common side effect of ACE inhibitors and has been identified as a possible risk factor for angioedema.

OBJECTIVE: We compared characteristics between patients with ACE inhibitor-induced angioedema and cough with the aim of identifying risk factors that differ between these adverse events.

METHODS: Data on patients with angioedema or cough induced by ACE inhibitors were collected from the Swedish database of spontaneously reported ADRs or from collaborating clinicians. Wilcoxon rank sum test, Fisher's exact test, and odds ratios (ORs) with 95% CIs were used to test for between-group differences. The significance threshold was set to P <0.00128 to correct for multiple comparisons.

RESULTS: Clinical characteristics were compared between 168 patients with angioedema and 121 with cough only. Smoking and concomitant selective calcium channel blocker treatment were more frequent among patients with angioedema than cough: OR = 4.3, 95% CI = 2.1-8.9, P = 2.2 × 10(-5), and OR = 3.7, 95% CI = 2.0-7.0, P = 1.7 × 10(-5). Angioedema cases were seen more often in male patients (OR = 2.2, 95% CI = 1.4-3.6, P = 1.3 × 10(-4)) and had longer time to onset and higher doses than those with cough ( P = 3.2 × 10(-10) and P = 2.6 × 10(-4)). A multiple model containing the variables smoking, concurrent calcium channel blocker treatment, male sex, and time to onset accounted for 26% of the variance between the groups.

CONCLUSION: Smoking, comedication with selective calcium channel blockers, male sex, and longer treatment time were associated with ACE inhibitor-induced angioedema rather than cough.

Keywords
ACE inhibitors, adult medicine, adverse drug reactions, calcium-channel blockers, clinical pharmacology, drug safety, interactions, medication safety, pulmonary
National Category
Pharmacology and Toxicology
Identifiers
urn:nbn:se:uu:diva-316913 (URN)10.1177/1060028016682251 (DOI)000396799400003 ()27889699 (PubMedID)
Funder
Swedish Research CouncilSwedish Heart Lung Foundation
Available from: 2017-03-08 Created: 2017-03-08 Last updated: 2018-02-01Bibliographically approved
Karawajczyk, M. & Larsson, A. (2017). Point-of-care instruments need to offer high levels of accuracy [Letter to the editor]. Acta Paediatrica, 106(10), Article ID 1707.
Open this publication in new window or tab >>Point-of-care instruments need to offer high levels of accuracy
2017 (English)In: Acta Paediatrica, ISSN 0803-5253, E-ISSN 1651-2227, Vol. 106, no 10, article id 1707Article in journal, Letter (Other academic) Published
National Category
Clinical Laboratory Medicine
Identifiers
urn:nbn:se:uu:diva-329212 (URN)10.1111/apa.14003 (DOI)000409348400029 ()28752551 (PubMedID)
Available from: 2017-09-10 Created: 2017-09-10 Last updated: 2018-03-19Bibliographically approved
Karawajczyk, M., Haile, S., Grabski, M. & Larsson, A. (2017). The HemoCue WBC DIFF system could be used for leucocyte and neutrophil counts but not for full differential counts. Acta Paediatrica, 106(6), 974-978
Open this publication in new window or tab >>The HemoCue WBC DIFF system could be used for leucocyte and neutrophil counts but not for full differential counts
2017 (English)In: Acta Paediatrica, ISSN 0803-5253, E-ISSN 1651-2227, Vol. 106, no 6, p. 974-978Article in journal (Refereed) Published
Abstract [en]

AIM: The aim of this study was to evaluate the HemoCue WBC DIFF system for point of care testing of fingerstick samples from paediatric patients.

METHODS: We analysed 158 white blood cell counts on both the point of care HemoCue WBC DIFF instrument and the Cell Dyn Sapphire cell counter used by our central laboratory and compared the results. The measurements were performed using fingerstick samples drawn by nurses working in paediatric emergency and paediatric oncology units.

RESULTS: There was good agreement between the two instruments for white blood cell and neutrophil counts. The correlation was weaker for lymphocytes and the correlations were poor for monocytes and eosinophils. The HemoCue WBC DIFF flagged 56 of the 148 capillary drawn samples as abnormal, but none of the 10 venously collected samples. Only two of the flagged samples differed significantly between the instruments, with regard to the cell counts.

CONCLUSION: The correlations between the white blood cell counts and neutrophil counts in this real life study were good enough to diagnose children in emergency department and oncology unit settings. However, the high number of pathological flags from fingerstick samples, which made reruns necessary, limited the usefulness of the instrument.

Keywords
Evaluation method, Neutrophil count, Paediatric care, Point of care test, White blood cell count
National Category
Pediatrics
Identifiers
urn:nbn:se:uu:diva-316475 (URN)10.1111/apa.13790 (DOI)000401011500021 ()28218961 (PubMedID)
Available from: 2017-03-01 Created: 2017-03-01 Last updated: 2017-06-27Bibliographically approved
Eriksson, O., Håkansson, L. D., Karawajczyk, M. & Garwicz, D. (2015). Neutrophil CD64 expression - comparison of two different flow cytometry protocols on EPICs MCL and the Leuko64 (TM) assay on a Celldyn Sapphire haematology analyser. Scandinavian Journal of Clinical and Laboratory Investigation, 75(5), 428-433
Open this publication in new window or tab >>Neutrophil CD64 expression - comparison of two different flow cytometry protocols on EPICs MCL and the Leuko64 (TM) assay on a Celldyn Sapphire haematology analyser
2015 (English)In: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, E-ISSN 1502-7686, Vol. 75, no 5, p. 428-433Article in journal (Refereed) Published
Abstract [en]

Objective. To evaluate the Trillium Diagnostics Leuko64 (TM) assay on Abbott Celldyn Sapphire haematology analyser compared to two flow cytometry protocols on Beckman Coulter EPICS MCL flow cytometer. Materials and methods. CD64 expression on neutrophils was determined by two flow cytometry protocols and by a commercial assay on an automatic haematology analyser. The inclusion of study subjects was based on elevated procalcitonin (PCT) values, identifying patients where a systemic infection was suspected. Healthy blood donors were used as a reference group. Results. Statistically significant correlations between the Trillium Diagnostics Leuko64 (TM) assay and the flow cytometry methods were found when measuring neutrophil CD64 expression. Conclusions. The good correlation between a reference method and an automated haematology analyser method for CD64 expression on neutrophils supports introduction of the latter assay for routine use as an independent biomarker of bacterial infection and inflammation.

Keywords
CD64, flow cytometry, neutrophils, method comparison
National Category
Infectious Medicine
Identifiers
urn:nbn:se:uu:diva-262972 (URN)10.3109/00365513.2015.1031690 (DOI)000360177600012 ()25874478 (PubMedID)
Available from: 2015-09-23 Created: 2015-09-23 Last updated: 2017-12-01Bibliographically approved
Vahlquist, A., Håkansson, L. D., Rönnblom, L., Karawajczyk, M., Fasth, A., van Gijn, M. E., . . . Venge, P. (2015). Recurrent Pyoderma Gangrenosum and Cystic Acne Associated with Leucocyte Adhesion Deficiency due to Novel Mutations in ITGB2: Successful Treatment with Infliximab and Adalimumab. Acta Dermato-Venereologica, 95(3), 349-351
Open this publication in new window or tab >>Recurrent Pyoderma Gangrenosum and Cystic Acne Associated with Leucocyte Adhesion Deficiency due to Novel Mutations in ITGB2: Successful Treatment with Infliximab and Adalimumab
Show others...
2015 (English)In: Acta Dermato-Venereologica, ISSN 0001-5555, E-ISSN 1651-2057, Vol. 95, no 3, p. 349-351Article in journal (Refereed) Published
National Category
Dermatology and Venereal Diseases
Identifiers
urn:nbn:se:uu:diva-251855 (URN)10.2340/00015555-1929 (DOI)000351249600022 ()
Available from: 2015-04-28 Created: 2015-04-24 Last updated: 2017-12-04Bibliographically approved
Wadelius, M., Marshall, S. E., Islander, G., Nordang, L., Karawajczyk, M., Yue, Q.-Y., . . . Palmer, C. N. (2014). Phenotype Standardization of Angioedema in the Head and Neck Region Caused by Agents Acting on the Angiotensin System. Clinical Pharmacology and Therapeutics, 96(4), 477-481
Open this publication in new window or tab >>Phenotype Standardization of Angioedema in the Head and Neck Region Caused by Agents Acting on the Angiotensin System
Show others...
2014 (English)In: Clinical Pharmacology and Therapeutics, ISSN 0009-9236, E-ISSN 1532-6535, Vol. 96, no 4, p. 477-481Article, review/survey (Refereed) Published
Abstract [en]

Angioedema is a potentially life-threatening adverse reaction to angiotensin-converting enzyme inhibitors and angiotensin receptor blockers. To study the genetic etiology of this rare adverse event, international consortia and multicenter recruitment of patients are needed. To reduce patient heterogeneity, we have standardized the phenotype. In brief, it comprises swelling in the head and neck region that first occurs during treatment. It should not coincide with urticaria or have another likely cause such as hereditary angioedema.

National Category
Pharmacology and Toxicology
Identifiers
urn:nbn:se:uu:diva-236046 (URN)10.1038/clpt.2014.138 (DOI)000342675400026 ()
Funder
Swedish Research Council, 523-2008-5568
Available from: 2014-11-18 Created: 2014-11-12 Last updated: 2018-01-11Bibliographically approved
Karawajczyk, M., Peterson, C. G. B., Venge, P. & Garcia, R. C. (2013). An Extragranular Compartment of Blood Eosinophils Contains Eosinophil Protein X/Eosinophil-Derived Neurotoxin (EPX/EDN). Inflammation, 36(2), 320-329
Open this publication in new window or tab >>An Extragranular Compartment of Blood Eosinophils Contains Eosinophil Protein X/Eosinophil-Derived Neurotoxin (EPX/EDN)
2013 (English)In: Inflammation, ISSN 0360-3997, E-ISSN 1573-2576, Vol. 36, no 2, p. 320-329Article in journal (Refereed) Published
Abstract [en]

Serum and plasma profiles of eosinophil protein X (EPX/EDN) and those of other eosinophil proteins differ in various conditions, suggesting a different mobilisation from storage granules. This work studied the subcellular localisation of EPX/EDN in non-primed and in vivo primed blood eosinophils from healthy and allergic subjects, during and out of the pollen season. Primed eosinophils contain easily mobilisable secretory proteins. By fractionation on sucrose density gradients, EPX/EDN localised in the specific granules as well as in a cytoplasmic extra-granular compartment of low equilibrium density that partially overlapped with vesicular structures, cytosolic proteins and plasma membranes. This compartment was clearly separate from the low density peak of ECP that increases during the pollen season. There were no significant differences in the amounts of EPX/EDN present in the low density peak of healthy and allergic subjects. Immuno-gold labelling electron microscopy showed EPX/EDN in specific granules, cytoplasm and associated to plasma membranes. In conclusion, substantial amounts of EPX/EDN localise in an extra-granular, low equilibrium density compartment of human eosinophils.

Keywords
intracellular localisation, sucrose density gradients, immunoelectron microscopy, pollen allergy
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-198377 (URN)10.1007/s10753-012-9549-z (DOI)000316020100008 ()
Available from: 2013-04-15 Created: 2013-04-15 Last updated: 2017-12-06Bibliographically approved
Karawajczyk, M., Ramklint, M. & Larsson, A. (2008). Reduced cystatin C-estimated GFR and increased creatinine-estimated GFR in comparison with iohexol-estimated GFR in a hyperthyroid patient: A case report. Journal of Medical Case Reports, 2(66)
Open this publication in new window or tab >>Reduced cystatin C-estimated GFR and increased creatinine-estimated GFR in comparison with iohexol-estimated GFR in a hyperthyroid patient: A case report
2008 (English)In: Journal of Medical Case Reports, ISSN 1752-1947, Vol. 2, no 66Article in journal (Other academic) Published
Abstract [en]

ABSTRACT: INTRODUCTION: Estimation of the glomerular filtration rate (GFR) is essential for the evaluation of patients with kidney disease, and for treating patients with drugs that are eliminated from the circulation by the kidneys. Cystatin C has been shown to be superior to creatinine for estimating GFR in several studies. However, studies showing that thyroid function has an impact on cystatin C have not addressed the question of whether the changes in cystatin C levels are due to changes in GFR or in cystatin C synthesis. CASE PRESENTATION: We report an account of a hyperthyroid patient with a discrepancy between the GFR estimates from cystatin C and creatinine. The cystatin C concentration (1.36 mg/L) was higher and gave an estimated GFR which was lower (51 mL/min/1.73 m2), while the creatinine concentration was lower (36 mumol/L) and gave a corresponding creatinine-estimated GFR that was higher (145 mL/min/1.73 m2) than the iohexol-estimated GFR (121 mL/min/1.73 m2) during the hyperthyroid period. After thyroidectomy, the creatinine concentration was 36 mumol/L and creatinine-estimated GFR was calculated as 73 mL/min/1.73 m2, while the cystatin C concentration and cystatin C-calculated GFR was 0.78 mg/L and 114 mL/min/1.73 m2, respectively. CONCLUSION: In contrast to creatinine, cystatin C levels rose in the hyperthyroid state as compared to the euthyroid state. The cystatin C-estimated GFR was reduced compared to the iohexol-estimated GFR. This patient case shows that the hyperthyroid-associated changes in cystatin C levels are not due to changes in GFR. Thyroid function should thus be considered when both cystatin C and creatinine are used as markers of kidney function.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-16913 (URN)10.1186/1752-1947-2-66 (DOI)18307770 (PubMedID)
Available from: 2008-06-10 Created: 2008-06-10Bibliographically approved
Organisations

Search in DiVA

Show all publications