uu.seUppsala University Publications
Change search
Link to record
Permanent link

Direct link
BETA
Ottosson, Jenny
Alternative names
Publications (1 of 1) Show all publications
Steen, J., Ramström, M., Uhlén, M., Hober, S. & Ottosson, J. (2009). Automated sample preparation method for mass spectrometry analysis on recombinant proteins. Journal of Chromatography A, 1216(20), 4457-4464
Open this publication in new window or tab >>Automated sample preparation method for mass spectrometry analysis on recombinant proteins
Show others...
2009 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1216, no 20, p. 4457-4464Article in journal (Refereed) Published
Abstract [en]

A completely automated procedure for the purification and desalting of proteins with a polyhistidine purification tag prior to mass spectrometry analysis is presented. The system is ideal for rapid quality control and optimization studies and it provides researchers with a straightforward, reliable tool for studies of recombinant proteins. Forty-eight samples can be prepared within 4.5 h and only small cultivation and buffer volumes are needed. In this proof of concept, 19,000-35,000 Da recombinant proteins from both crude and clarified cell lysates were successfully prepared for subsequent analysis by electrospray ionization anti matrix-assisted laser desorption/ionization mass spectrometry as well as by gel electrophoresis.

Keywords
Mass spectrometry, High-throughput, Automated sample preparation, Recombinant proteins, IMAC, RPLC, affinity-chromatography, purification
National Category
Natural Sciences
Identifiers
urn:nbn:se:uu:diva-172141 (URN)10.1016/j.chroma.2009.03.041 (DOI)000265956100021 ()
Note
QC 20100525Available from: 2010-08-05 Created: 2012-04-02 Last updated: 2017-12-07

Search in DiVA

Show all publications