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Fredricsson, Annika
Publications (4 of 4) Show all publications
Lampinen, M., Fredricsson, A., Vessby, J., Martinez, J. F., Wanders, A., Rorsman, F. & Carlson, M. (2018). Downregulated eosinophil activity in ulcerative colitis with concomitant primary sclerosing cholangitis. Paper presented at 10th Biennial Symposium of the International-Eosinophil-Society, JUL 19-23, 2017, Gothenburg, SWEDEN. Journal of Leukocyte Biology, 104(1), 173-183
Open this publication in new window or tab >>Downregulated eosinophil activity in ulcerative colitis with concomitant primary sclerosing cholangitis
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2018 (English)In: Journal of Leukocyte Biology, ISSN 0741-5400, E-ISSN 1938-3673, Vol. 104, no 1, p. 173-183Article in journal (Refereed) Published
Abstract [en]

Primary sclerosing cholangitis (PSC) is a chronic bile duct inflammation strongly connected to ulcerative colitis (UC). PSC is associated with an increased risk of colon cancer, but the link between the intestinal and the bile duct inflammation is still unknown. Also, the involvement of intestinal immune cells in the pathogenesis of PSC remains to be determined. The eosinophil granulocyte is one of the immune cells implicated in the inflammatory process of ulcerative colitis. This study was performed to determine how the accumulation and activation of intestinal eosinophils may differ between UC with and without concomitant PSC, and how this may be influenced by the cytokine/chemokine profile of the intestinal compartment. Eosinophils from peripheral blood and multiple parts of the colon were analyzed by flow cytometry. The intestinal level of inflammatory mediators was assessed using a multiplex proximity extension assay and a quantitative immunoassay. We found that colonic eosinophils were more abundant in both UC and PSC-UC compared with controls, but that their expression of activation markers was significantly increased in UC only. The colonic level of pro-inflammatory cytokines was increased in active UC but not in PSC-UC. In conclusion, we show for the first time that eosinophil activation phenotype discriminates between UC and PSC-UC, and that this may depend on the local cytokine profile of the colonic mucosa. Lower expression of activation markers on eosinophils in UC with concomitant PSC may depend on the local protein profile of the colonic mucosa.

Place, publisher, year, edition, pages
WILEY, 2018
Keywords
eotaxin-1, flow cytometry, IL-33, proximity extension assay
National Category
Gastroenterology and Hepatology
Identifiers
urn:nbn:se:uu:diva-358268 (URN)10.1002/JLB.3MA0517-175R (DOI)000435944300016 ()29603385 (PubMedID)
Conference
10th Biennial Symposium of the International-Eosinophil-Society, JUL 19-23, 2017, Gothenburg, SWEDEN
Funder
Stiftelsen Olle Engkvist Byggmästare
Available from: 2018-08-29 Created: 2018-08-29 Last updated: 2018-08-29Bibliographically approved
Lampinen, M., Fredricsson, A., Vessby, J., Wanders, A., Rorsman, F. & Carlson, M. (2016). Expression of the liver homing receptor CXCR3+on colonic CD8+T lymphocytes in patients with primary sclerosing cholangitis provides a possible link between colonic and biliary duct inflammation. Journal of Crohn's & Colitis, 10, S109-S109
Open this publication in new window or tab >>Expression of the liver homing receptor CXCR3+on colonic CD8+T lymphocytes in patients with primary sclerosing cholangitis provides a possible link between colonic and biliary duct inflammation
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2016 (English)In: Journal of Crohn's & Colitis, ISSN 1873-9946, E-ISSN 1876-4479, Vol. 10, p. S109-S109Article in journal, Meeting abstract (Other academic) Published
National Category
Gastroenterology and Hepatology
Identifiers
urn:nbn:se:uu:diva-299652 (URN)000374496600160 ()
Available from: 2016-07-25 Created: 2016-07-25 Last updated: 2017-11-28Bibliographically approved
Helmersson-Karlqvist, J., Karlsson, B., Fredricsson, A. & Larsson, A. (2014). Evaluation of the Alere D-dimer test for point of care testing. Journal of Thrombosis and Thrombolysis, 38(2), 250-252
Open this publication in new window or tab >>Evaluation of the Alere D-dimer test for point of care testing
2014 (English)In: Journal of Thrombosis and Thrombolysis, ISSN 0929-5305, E-ISSN 1573-742X, Vol. 38, no 2, p. 250-252Article in journal (Refereed) Published
Abstract [en]

The primary care regularly sees patients that have symptoms that could be due to thromboembolic diseases. It would be valuable to be able to rule out deep venous thrombosis or pulmonary embolism using Wells score and a negative D-dimer testing already at the primary care unit. This requires a validated D-dimer assay suitable for primary care use. We compared D-dimer results obtained with the new point of care analyzer Alere Triage(®) and the central hospital laboratory STA-R Evolution analyzer from the same patient samples (n = 102). We also calculated the total coefficient of variation (CV) for the Alere method. The two methods showed a good linear correlation (R(2) = 0.977) and a slope of 0.975. CV for the Alere D-dimer method was well below 10 %. The study shows that the Alere D-dimer assay and the central laboratory standard assay show similar results. We suggest that the Alere D-dimer assay could be used in primary care in combination with Wells score to reduce referrals to the emergency unit.

National Category
Clinical Laboratory Medicine
Identifiers
urn:nbn:se:uu:diva-220521 (URN)10.1007/s11239-013-1043-4 (DOI)000338706400018 ()24381121 (PubMedID)
Available from: 2014-03-17 Created: 2014-03-17 Last updated: 2017-12-05Bibliographically approved
Noraddin, F. H., Flodin, M., Fredricsson, A., Sohrabian, A. & Larsson, A. (2012). Measurement of urinary cystatin C with a particle-enhanced turbidimetric immunoassay on architect ci8200. Journal of clinical laboratory analysis (Print), 26(5), 358-364
Open this publication in new window or tab >>Measurement of urinary cystatin C with a particle-enhanced turbidimetric immunoassay on architect ci8200
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2012 (English)In: Journal of clinical laboratory analysis (Print), ISSN 0887-8013, E-ISSN 1098-2825, Vol. 26, no 5, p. 358-364Article in journal (Refereed) Published
Abstract [en]

BACKGROUND:

Cystatin C is a low-molecular-weight protein that is freely filtered by the glomerulus and catabolized after reabsorption by the proximal tubular cells in healthy subjects. Urinary cystatin C is a potential biomarker for tubular damage including acute kidney injury (AKI) in the acute phase when patients are submitted to the intensive care unit.

METHODS:

The aim of this study was to perform a method validation of urinary analysis of cystatin C by particle-enhanced turbidimetric immunoassay (PETIA) on a high-throughput chemical analyzer. Total assay time was 10 min. The antigen excess, linearity, lower limit of quantification (LoQ), recovery, assay precision, stability, and interference caused by hemoglobin were evaluated.

RESULTS:

The LoQ was calculated to 0.020 mg/l with a coefficient of variation (CV) ≤ 10%. No hook effect was observed and the assay was linear over the studied interval less than 0.020-0.950 mg/l with a regression of R(2) = 0.9994. The assay had a recovery between 93-100% and the assay precision had a total CV of less than 3.5%. Cystatin C was stable for 3 days in room temperature and 14 days in +4C. The assay did not show any major interference with hemoglobin at a hemoglobin concentration of 10 g/L. The reference interval for urine cystatin C was less than 0.166 mg/l.

CONCLUSION:

The urinary cystatin C PETIA showed good precision and performance characteristics including short test turnaround times that are necessary qualifications for a biomarker at a routine laboratory.

National Category
Urology and Nephrology
Identifiers
urn:nbn:se:uu:diva-182321 (URN)10.1002/jcla.21531 (DOI)000309064800009 ()23001981 (PubMedID)
Available from: 2012-10-09 Created: 2012-10-09 Last updated: 2017-12-07Bibliographically approved
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