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Karypidis, Helena
Publications (4 of 4) Show all publications
Haroun, S., Altmäe, S., Karypidis, H., Kuningas, M., Landgren, B.-M., Åkerud, H., . . . Stavreus-Evers, A. (2014). Association between trefoil factor 3 gene variants and idiopathic recurrent spontaneous abortion. Reproductive Biomedicine Online, 29(6), 737-44
Open this publication in new window or tab >>Association between trefoil factor 3 gene variants and idiopathic recurrent spontaneous abortion
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2014 (English)In: Reproductive Biomedicine Online, ISSN 1472-6483, E-ISSN 1472-6491, Vol. 29, no 6, p. 737-44Article in journal (Refereed) Published
Abstract [en]

Trefoil factor 3 (TFF3) gene is an inflammatory mediator expressed in human endometrium during the window of implantation. The aim of this study was to evaluate the possible genetic association of TFF3 variants in recurrent spontaneous abortion. Women with a history of recurrent spontaneous abortion (n = 164) and healthy pregnant women (n = 143) were genotyped for five TFF3 polymorphisms (rs225439 G/A, rs533093 C/T, rs225361 A/G, rs11701143 T/C and rs77436142 G/C). In addition, haplotypes formed within the gene were analysed. Within the recurrent spontaneous abortion group, women who at some point had given birth and childless women had 4.19 ± 1.75 and 5.34 ± 3.42 consecutive spontaneous abortions, respectively. Women who had experience recurrent spontaneous abortions had a lower allele frequency of the rs11701143 promoter region minor C allele compared with fertile women (0.02 versus 0.05, P = 0.015). Patients with rs225361 AG genotype had significantly more successful pregnancies before spontaneous abortion than those with homozygous AA and GG genotypes (P = 0.014). No significant differences in haplotype frequencies between patients and controls were detected. Possible genetic risk factors identified that might contribute to the pathogenesis of idiopathic recurrent spontaneous abortion were TFF3 gene variants.

National Category
Obstetrics, Gynecology and Reproductive Medicine
Identifiers
urn:nbn:se:uu:diva-238226 (URN)10.1016/j.rbmo.2014.08.007 (DOI)000345759300012 ()25444508 (PubMedID)
Available from: 2014-12-10 Created: 2014-12-10 Last updated: 2017-12-05Bibliographically approved
Elenis, E., Lindgren, K. E., Karypidis, H., Skalkidou, A., Hosseini, F., Bremme, K., . . . Åkerud, H. (2014). The histidine-rich glycoprotein A1042G polymorphism and recurrent miscarriage: a pilot study. Reproductive Biology and Endocrinology, 12, 70
Open this publication in new window or tab >>The histidine-rich glycoprotein A1042G polymorphism and recurrent miscarriage: a pilot study
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2014 (English)In: Reproductive Biology and Endocrinology, ISSN 1477-7827, E-ISSN 1477-7827, Vol. 12, p. 70-Article in journal (Refereed) Published
Abstract [en]

Background: Histidine-rich Glycoprotein (HRG) has previously been shown to have an impact on implantation and fertility. The aim of this study was to investigate if there is an association between the HRG A1042G single nucleotide polymorphism (SNP) and recurrent miscarriage. Methods: The study was designed as a case-control study and the women were included at University Hospitals in Sweden. 186 cases with recurrent miscarriage were compared with 380 pregnant controls with no history of miscarriage. Each woman was genotyped for the HRG A1042G SNP. Results: The results indicated that the frequency of heterozygous HRG A1042G carriers was higher among controls compared to cases (34.7% vs 26.3%; p < 0.05). In a bivariate regression analysis, a negative association was found between recurrent miscarriage and heterozygous A/G carriers both in the entire study population (OR 0.67, 95% CI 0.45 - 0.99; p < 0.05) as well as in a subgroup of women with primary recurrent miscarriage (OR 0.37, 95% CI 0.16 - 0.84; p < 0.05). These results remained even after adjustment for known confounders such as age, BMI and thyroid disease (OR 0.36, 95% CI 0.15 - 0.84; p < 0.05). Conclusions: Women who are heterozygous carriers of the HRG A1042G SNP suffer from recurrent miscarriage more seldom than homozygous carriers. Thus, analysis of the HRG A1042G SNP might be of importance for individual counseling regarding miscarriage.

Keywords
Histidine-rich glycoprotein, Recurrent miscarriage, Single nucleotide polymorphism
National Category
Obstetrics, Gynecology and Reproductive Medicine
Identifiers
urn:nbn:se:uu:diva-231117 (URN)10.1186/1477-7827-12-70 (DOI)000339590200001 ()25064236 (PubMedID)
Available from: 2014-09-05 Created: 2014-09-04 Last updated: 2017-12-05Bibliographically approved
Lindgren, K. E., Kårehed, K., Karypidis, H., Hosseini, F., Bremme, K., Landgren, B.-M., . . . Åkerud, H. (2013). Histidine-rich glycoprotein gene polymorphism in patients with recurrent miscarriage. Acta Obstetricia et Gynecologica Scandinavica, 92(8), 974-977
Open this publication in new window or tab >>Histidine-rich glycoprotein gene polymorphism in patients with recurrent miscarriage
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2013 (English)In: Acta Obstetricia et Gynecologica Scandinavica, ISSN 0001-6349, E-ISSN 1600-0412, Vol. 92, no 8, p. 974-977Article in journal (Refereed) Published
Abstract [en]

Association between the histidine-rich glycoprotein (HRG) C633T single nucleotide polymorphism (SNP) and recurrent miscarriage was investigated in a case-control study. The cases constituted 187 women with recurrent miscarriage that were compared with 395 controls who had delivered a child and had no history of miscarriage. Blood samples were collected from each woman, genomic DNA was extracted and genotyped for the HRG C633T SNP. In the whole study population, the percentage of miscarriage was the same, regardless of genotype (C/C 31.2%, C/T 32.9% and T/T 32.5%). However, an association between homozygous T/T carriers and recurrent miscarriage was detected in a subgroup of women with primary recurrent miscarriage (odds ratio 2.44, 95% CI 1.01-5.92). Our results indicate an important role for the HRG C633T SNP in the occurrence of recurrent miscarriage.

Place, publisher, year, edition, pages
John Wiley & Sons, 2013
Keywords
Genotype, histidine-rich glycoprotein, infertility, recurrent miscarriage, single nucleotide polymorphism
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-207015 (URN)10.1111/aogs.12155 (DOI)000321820100015 ()
Available from: 2013-09-10 Created: 2013-09-09 Last updated: 2017-12-06
Granfors, M., Karypidis, H., Hosseini, F., Skjöldebrand-Sparre, L., Stavreus-Evers, A., Bremme, K., . . . Akerud, H. (2012). Phosphodiesterase 8B gene polymorphism in women with recurrent miscarriage: A retrospective case control study.. BMC Medical Genetics, 13, 121
Open this publication in new window or tab >>Phosphodiesterase 8B gene polymorphism in women with recurrent miscarriage: A retrospective case control study.
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2012 (English)In: BMC Medical Genetics, ISSN 1471-2350, E-ISSN 1471-2350, Vol. 13, p. 121-Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Recurrent miscarriage affects approximately 1% of all couples. There is a known relation between hypothyroidism and recurrent miscarriage. Phosphodiesterase 8B (PDE8B) is a regulator of cyclic adenosine monophosphate (cAMP) with important influence on human thyroid metabolism. Single nucleotide polymorphism (SNP) rs 4704397 in the PDE8B gene has been shown to be associated with variations in serum Thyroid Stimulating Hormone (TSH) and thyroxine (T4) levels. The aim of this study was to investigate whether there is an association between the SNP rs 4704397 in the PDE8B gene and recurrent miscarriage. METHODS: The study was designed as a retrospective case control study. 188 cases with recurrent miscarriage were included and compared with 391 controls who had delivered at least once and with no history of miscarriage or assisted reproduction. RESULTS: No difference between cases and controls concerning age was found. Bivariate associations between homozygous A/A (OR 1.57, 95% CI 0.98-2.52) as well as G/G carriers (OR 1.52, 95% CI 1.02-2.25) of SNP rs 4704397 in PDE8B and recurrent miscarriage were verified (test for trend across all 3 genotypes, p = 0.059). After adjustment for known confounders such as age, BMI and smoking the association between homozygous A/A (AOR 1.63, 95% CI 1.01 - 2.64, p = 0.045) and G/G (AOR 1.52, 95% CI 1.02 - 2.27, p = 0.039) carriers of SNP rs 4704397 in PDE8B and recurrent miscarriage remained. CONCLUSIONS: Our findings suggest that there is an association between homozygous A/A as well as homozygous G/G carriers of SNP rs 4704397 in PDE8B and recurrent miscarriage.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-188671 (URN)10.1186/1471-2350-13-121 (DOI)000314113200001 ()23237535 (PubMedID)
Available from: 2012-12-18 Created: 2012-12-18 Last updated: 2017-12-06Bibliographically approved
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