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Publications (10 of 23) Show all publications
Gerecht, A., Supraha, L., Langer, G. & Henderiks, J. (2018). Phosphorus limitation and heat stress decrease calcification in Emiliania huxleyi. Biogeosciences, 15, 833-845
Open this publication in new window or tab >>Phosphorus limitation and heat stress decrease calcification in Emiliania huxleyi
2018 (English)In: Biogeosciences, ISSN 1726-4170, E-ISSN 1726-4189, Vol. 15, p. 833-845Article in journal (Refereed) Published
Abstract [en]

Calcifying haptophytes (coccolithophores) sequester carbon in the form of organic and inorganic cellular components (coccoliths). We examined the effect of phosphorus (P) limitation and heat stress on particulate organic and inorganic carbon (calcite) production in the coccolithophore Emiliania huxleyi. Both environmental stressors are related to rising CO2 levels and affect carbon production in marine microalgae, which in turn impacts biogeochemical cycling. Using semi-continuous cultures, we show that P-limitation and heat stress decrease the calcification rate in E. huxleyi. This could lessen the ballasting effect of coccoliths and weaken carbon export out of the photic zone. However, using batch cultures, we show that different culturing approaches (batch versus semi-continuous) induce different physiologies. This affects the ratio of inorganic (PIC) to organic (POC) carbon and complicates general predictions on the effect of P-limitation on the PIC / POC ratio. Furthermore, heat stress increases P-requirements in E. huxleyi, possibly leading to lower standing stocks in a warmer ocean, especially if this is linked to lower nutrient input. In summary, the predicted rise in global temperature and resulting decrease in nutrient availability may first of all decrease CO2 sequestration by coccolithophores through lower overall carbon production. Secondly, the export of carbon may be diminished by a decrease in calcification and a weaker coccolith ballasting effect.

National Category
Earth and Related Environmental Sciences
Identifiers
urn:nbn:se:uu:diva-321897 (URN)10.5194/bg-15-833-2018 (DOI)000424704700003 ()
Funder
Knut and Alice Wallenberg Foundation, KAW 2009.0287
Available from: 2017-05-11 Created: 2017-05-11 Last updated: 2018-03-21Bibliographically approved
Gran-Stadniczeñko, S., Supraha, L., Egge, E. D. & Edvardsen, B. (2017). Haptophyte diversity and vertical distribution explored by 18S and 28S ribosomal RNA gene metabarcoding and scanning electron microscopy. Journal of Eukaryotic Microbiology, 64(4), 514-532
Open this publication in new window or tab >>Haptophyte diversity and vertical distribution explored by 18S and 28S ribosomal RNA gene metabarcoding and scanning electron microscopy
2017 (English)In: Journal of Eukaryotic Microbiology, ISSN 1066-5234, E-ISSN 1550-7408, Vol. 64, no 4, p. 514-532Article in journal (Refereed) Published
Abstract [en]

Haptophyta encompasses more than 300 species of mostly marine pico- and nanoplanktonic flagellates. Our aims were to investigate the Oslofjorden haptophyte diversity and vertical distribution by metabarcoding, and to improve the approach to study haptophyte community composition, richness and proportional abundance by comparing two rRNA markers and scanning electron microscopy (SEM). Samples were collected in August 2013 at the Outer Oslofjorden, Norway. Total RNA/cDNA was amplified by haptophyte-specific primers targeting the V4 region of the 18S, and the D1-D2 region of the 28S rRNA. Taxonomy was assigned using curated haptophyte reference databases and phylogenetic analyses. Both marker genes showed Chrysochromulinaceae and Prymnesiaceae to be the families with highest number of Operational Taxonomic Units (OTUs), as well as proportional abundance. The 18S rRNA data setalso contained OTUs assigned to eight supported and defined clades consisting of environmental sequences only, possibly representing novel lineages from family to class. We also recorded new species for the area. Comparing coccolithophores by SEM with metabarcoding shows a good correspondence with the 18S rRNA gene proportional abundances. Our results contribute to link morphological and molecular data and 28S to 18S rRNA gene sequences of haptophytes without cultured representatives, and to improve metabarcoding methodology.

Keyword
Abundance, coccolithophores, high-throughput sequencing, Oslofjorden, phylogeny, richness
National Category
Biological Sciences Biological Systematics Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-302898 (URN)10.1111/jeu.12388 (DOI)000406102000010 ()27973742 (PubMedID)
Note

De två första författarna delar förstaförfattarskapet.

Available from: 2016-09-12 Created: 2016-09-12 Last updated: 2017-11-02Bibliographically approved
Bosak, S., Ljubesic, Z., Supraha, L., Neeley, A., Durkin, C. A., Estapa, M., . . . Cetinic, I. (2017). PHYTOPLANKTON SPECIES COMPOSITION CONTRIBUTING TO CARBON EXPORT - SEA TO SPACE PARTICLE INVESTIGATION. Phycologia, 56(4), 23-24
Open this publication in new window or tab >>PHYTOPLANKTON SPECIES COMPOSITION CONTRIBUTING TO CARBON EXPORT - SEA TO SPACE PARTICLE INVESTIGATION
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2017 (English)In: Phycologia, ISSN 0031-8884, E-ISSN 2330-2968, Vol. 56, no 4, p. 23-24Article in journal, Meeting abstract (Other academic) Published
National Category
Biological Sciences
Identifiers
urn:nbn:se:uu:diva-346763 (URN)000412892000045 ()
Available from: 2018-03-29 Created: 2018-03-29 Last updated: 2018-03-29Bibliographically approved
Korlević, M., Šupraha, L., Ljubešić, Z., Henderiks, J., Ciglenečki, I., Dautović, J. & Orlić, S. (2016). Bacterial diversity across a highly stratified ecosystem: A salt-wedge Mediterranean estuary. Systematic and Applied Microbiology, 39(6), 398-408
Open this publication in new window or tab >>Bacterial diversity across a highly stratified ecosystem: A salt-wedge Mediterranean estuary
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2016 (English)In: Systematic and Applied Microbiology, ISSN 0723-2020, E-ISSN 1618-0984, Vol. 39, no 6, p. 398-408Article in journal (Refereed) Published
Abstract [en]

Highly stratified Mediterranean estuaries are unique environments where the tidal range is low and the tidal currents are almost negligible. The main characteristics of these environments are strong salinity gradients and other environmental parameters. In this study, 454 pyrosequencing of the 16S rRNA gene in combination with catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) was used to estimate the bacterial diversity across the Krka estuary in February and July 2013. The comparison of the data derived from these two techniques resulted in a significant but weak positive correlation (R = 0.28) indicating a substantial difference in the bacterial community structure, depending on the applied method. The phytoplankton bloom observed in February was identified as one of the main factors shaping the bacterial community structure between the two environmentally contrasting sampling months. Roseobacter, Bacteroidetes and Gammaproteobacteria differed substantially between February and July. Typical freshwater bacterial classes (Actinobacteria and Betaproteobacteria) showed strong vertical distribution patterns depending on the salinity gradient. Cyanobacteria decreased in abundance in February due to competition with phytoplankton, while the SAR11 clade increased its abundance in July as a result of a better adaptation toward more oligotrophic conditions. The results provided the first detailed insight into the bacterial diversity in a highly stratified Mediterranean karstic estuary.

Keyword
16S amplicon, CARD FISH, Estuaries, Gradient, Phytoplankton bloom, Diversity
National Category
Microbiology Ecology
Research subject
Microbiology
Identifiers
urn:nbn:se:uu:diva-301115 (URN)10.1016/j.syapm.2016.06.006 (DOI)000383305000006 ()27475818 (PubMedID)
Funder
Knut and Alice Wallenberg Foundation, 2009.0287
Available from: 2016-08-18 Created: 2016-08-18 Last updated: 2017-11-28Bibliographically approved
Gran Stadniczenko, S., Šupraha, L., Egge, E. & Edvardsen, B. (2016). Comparing high-throughput sequencing and scanning electron microscopy to investigate haptophyte communities in Oslofjorden (Skagerrak). In: : . Paper presented at EMBO/EMBL Symposium: A New Age of Discovery for Aquatic Microeukaryotes.
Open this publication in new window or tab >>Comparing high-throughput sequencing and scanning electron microscopy to investigate haptophyte communities in Oslofjorden (Skagerrak)
2016 (English)Conference paper, Poster (with or without abstract) (Refereed)
National Category
Microbiology Ecology
Identifiers
urn:nbn:se:uu:diva-275746 (URN)
Conference
EMBO/EMBL Symposium: A New Age of Discovery for Aquatic Microeukaryotes
Available from: 2016-02-05 Created: 2016-02-05 Last updated: 2016-02-05
Šupraha, L., Gerecht, A. C., Probert, I. & Henderiks, J. (2016). The adaptive strategies of coccolithophores and their biogeochemical implications. In: : . Paper presented at EMBO/EMBL Symposium: A New Age of Discovery for Aquatic Microeukaryotes.
Open this publication in new window or tab >>The adaptive strategies of coccolithophores and their biogeochemical implications
2016 (English)Conference paper, Poster (with or without abstract) (Refereed)
National Category
Evolutionary Biology
Identifiers
urn:nbn:se:uu:diva-275745 (URN)
Conference
EMBO/EMBL Symposium: A New Age of Discovery for Aquatic Microeukaryotes
Available from: 2016-02-05 Created: 2016-02-05 Last updated: 2016-02-05
Mejdandzic, M., Mihanovic, H., Silovic, T., Henderiks, J., Supraha, L., Polovic, D., . . . Ljubesic, Z. (2015). Biomarker Pigment Divinyl Chlorophyll a as a Tracer of Water Masses?. Paper presented at 6th European Phycological Congress (EPC6), 23-28 August, 2015, London, UK. European journal of phycology, 50(Suppl. 1), 162-163
Open this publication in new window or tab >>Biomarker Pigment Divinyl Chlorophyll a as a Tracer of Water Masses?
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2015 (English)In: European journal of phycology, ISSN 0967-0262, E-ISSN 1469-4433, Vol. 50, no Suppl. 1, p. 162-163Article in journal, Meeting abstract (Other academic) Published
National Category
Psychology
Identifiers
urn:nbn:se:uu:diva-264493 (URN)000360244400293 ()
Conference
6th European Phycological Congress (EPC6), 23-28 August, 2015, London, UK
Note

Meeting Abstract: 8PO.12

Available from: 2015-10-19 Created: 2015-10-13 Last updated: 2017-12-01Bibliographically approved
Šupraha, L., Ljubešić, Z. & Henderiks, J. (2015). Combination coccospheres from the eastern Adriatic coast (Mediterranean Sea). In: : . Paper presented at 15th International Nannoplankton Association Meeting, Bohol Island, Philippines (pp. 81). , 35
Open this publication in new window or tab >>Combination coccospheres from the eastern Adriatic coast (Mediterranean Sea)
2015 (English)Conference paper, Poster (with or without abstract) (Other academic)
National Category
Earth and Related Environmental Sciences
Identifiers
urn:nbn:se:uu:diva-253372 (URN)
Conference
15th International Nannoplankton Association Meeting, Bohol Island, Philippines
Available from: 2015-05-26 Created: 2015-05-26 Last updated: 2015-05-26
Šupraha, L., Gerecht, A. C., Probert, I. & Henderiks, J. (2015). Eco-physiological adaptation shapes the response of calcifying algae to nutrient limitation. Scientific Reports, 5, Article ID 16499.
Open this publication in new window or tab >>Eco-physiological adaptation shapes the response of calcifying algae to nutrient limitation
2015 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 5, article id 16499Article in journal (Refereed) Published
Abstract [en]

The steady increase in global ocean temperature will most likely lead to nutrient limitation in the photic zone. This will impact the physiology of marine algae, including the globally important calcifying coccolithophores. Understanding their adaptive patterns is essential for modelling carbon production in a low-nutrient ocean. We investigated the physiology of Helicosphaera carteri, a representative of the abundant but under-investigated flagellated functional group of coccolithophores. Two strains isolated from contrasting nutrient regimes (South Atlantic and Mediterranean Sea) were grown in phosphorus-replete and phosphorus-limited batch cultures. While growing exponentially in a phosphorus-replete medium, the Mediterranean strain exhibited on average 24% lower growth rate, 36% larger coccosphere volume and 21% lower particulate inorganic carbon (PIC) production than the Atlantic strain. Under phosphorus limitation, the same strain was capable of reaching a 2.6 times higher cell density than the Atlantic strain due to lower phosphorus requirements. These results suggest that local physiological adaptation can define the performance of this species under nutrient limitation.

National Category
Ecology Cell Biology Evolutionary Biology Earth and Related Environmental Sciences
Identifiers
urn:nbn:se:uu:diva-253354 (URN)10.1038/srep16499 (DOI)000364487200001 ()26560531 (PubMedID)
Funder
Knut and Alice Wallenberg FoundationThe Royal Swedish Academy of Sciences
Available from: 2015-05-26 Created: 2015-05-26 Last updated: 2017-12-04Bibliographically approved
Bosak, S., Šupraha, L., Nanjappa, D., Kooistra, W. H. .. & Sarno, D. (2015). Morphology and phylogeny of four species from the genus Bacteriastrum (Bacillariophyta). Phycologia, 54(2), 130-148
Open this publication in new window or tab >>Morphology and phylogeny of four species from the genus Bacteriastrum (Bacillariophyta)
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2015 (English)In: Phycologia, Vol. 54, no 2, p. 130-148Article in journal (Refereed) Published
National Category
Biological Systematics
Identifiers
urn:nbn:se:uu:diva-253637 (URN)10.2216/14-62 (DOI)
Available from: 2015-05-29 Created: 2015-05-29 Last updated: 2015-05-29
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-2613-945x

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