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BETA
Lanekoff, Ingela, Assoc. Prof.ORCID iD iconorcid.org/0000-0001-9040-3230
Alternative names
Publications (10 of 38) Show all publications
Carter, S.-S., Atif, A. R., Kadekar, S., Lanekoff, I., Engqvist, H., Varghese, O. P., . . . Mestres, G. (2020). PDMS leaching and its implications for on-chip studies focusing on boneregeneration applications. Organs-on-a-Chip, 2(100004)
Open this publication in new window or tab >>PDMS leaching and its implications for on-chip studies focusing on boneregeneration applications
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2020 (English)In: Organs-on-a-Chip, ISSN 2666-1020, Vol. 2, no 100004Article in journal (Refereed) Published
Abstract [en]

Polydimethylsiloxane (PDMS) is among the most widely used materials for organ-on-chip systems. Despite itsmultiple beneficial characteristics from an engineering point of view, there is a concern about the effect of PDMSon the cells cultured in such devices. The aim of this study was to enhance the understanding of the effect of PDMSon cellular behavior in a context relevant for on-chip studies. The focus was put on an indirect effect of PDMS,namely leaching of uncrosslinked oligomers, particularly for bone regeneration applications. PDMS-based chipswere prepared and analyzed for the potential release of PDMS oligomers within the microfluidic channel whenkept at different flow rates. Leaching of uncrosslinked oligomers from PDMS was quantified as silicon concen-tration by inductively coupled plasma - optical emission spectrometry and further confirmed by mass spec-trometry. Subsequently, PDMS-leached media, with a silicon concentration matching the on-chip experiment,were prepared to study cell proliferation and osteogenic differentiation of MC3T3-E1 pre-osteoblasts and humanmesenchymal stem cells. The silicon concentration initially detected in the media was inversely proportional tothe tested flow rates and decreased to control levels within 52 h. In addition, by curing the material overnightinstead of 2 h, regardless of the curing temperature (65 and 80 C), a large reduction in silicon concentration wasfound, indicating the importance of the PDMS curing parameters. Furthermore, it was shown that PDMS oligo-mers enhanced the differentiation of MC3T3-E1 pre-osteoblasts, this being a cell type dependent effect as nochanges in cell differentiation were observed for human mesenchymal stem cells. Overall, this study illustrates theimportance of optimization steps when using PDMS devices for biological studies, in particular PDMS curingconditions and extensive washing steps prior to an experiment.

Place, publisher, year, edition, pages
Elsevier, 2020
Keywords
PDMS, Organs-on-chip, Human mesenchymal stem cells, Osteoblasts, Silicon
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-410262 (URN)10.1016/j.ooc.2020.100004 (DOI)
Funder
Swedish Research Council Formas, 2016-00781Swedish Research Council, 2017-05051Göran Gustafsson Foundation for Research in Natural Sciences and Medicine, 1841Knut and Alice Wallenberg Foundation, 2016-0112
Available from: 2020-05-13 Created: 2020-05-13 Last updated: 2020-05-15Bibliographically approved
Duncan, K. D., Fyrestam, J. & Lanekoff, I. (2019). Advances in mass spectrometry based single-cell metabolomics. The Analyst, 144(3), 782-793
Open this publication in new window or tab >>Advances in mass spectrometry based single-cell metabolomics
2019 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 144, no 3, p. 782-793Article, review/survey (Refereed) Published
Abstract [en]

Metabolomics has grown into a prominent field contributing to the molecular understanding of complex biological processes in both health and disease. Furthermore, single-cells are known to display metabolic differences between seemingly homogeneous populations of cells. Single-cell metabolomics attempts to analyze many cellular metabolites from single cells to understand phenotypic heterogeneity, which is a significant challenge due to the low analyte abundances and limited sample volumes. Label-free metabolite detection can be achieved with mass spectrometry, which is capable of simultaneously analyzing hundreds of metabolites. Herein, we review the recent advances in mass spectrometry based single-cell metabolomics, highlighting the current state-of-the-art within the last three years, and identify the challenges to move the field forward.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY, 2019
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-377492 (URN)10.1039/c8an01581c (DOI)000457394400004 ()30426983 (PubMedID)
Funder
Swedish Foundation for Strategic Research Swedish Research Council
Available from: 2019-02-20 Created: 2019-02-20 Last updated: 2019-02-20Bibliographically approved
Bergman, H.-M., Lindfors, L., Palm, F., Kihlberg, J. & Lanekoff, I. (2019). Metabolite aberrations in early diabetes detected in rat kidney using mass spectrometry imaging. Analytical and Bioanalytical Chemistry, 411(13), 2809-2816
Open this publication in new window or tab >>Metabolite aberrations in early diabetes detected in rat kidney using mass spectrometry imaging
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2019 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 411, no 13, p. 2809-2816Article in journal (Refereed) Published
Abstract [en]

Diabetic kidney disease is a serious complication of diabetes that can ultimately lead to end-stage renal disease. The pathogenesis of diabetic kidney disease is complex, and fundamental research is still required to provide a better understanding of the driving forces behind it. We report regional metabolic aberrations from an untargeted mass spectrometry imaging study of kidney tissue using an insulinopenic rat model of diabetes. Diabetes was induced by intravenous injection of streptozotocin, and kidneys were harvested 2weeks thereafter. Imaging was performed using nanospray desorption electrospray ionization connected to a high-mass-resolving mass spectrometer. No histopathological changes were observed in the kidney sections; however, mass spectrometry imaging revealed a significant increase in several 18-carbon unsaturated non-esterified fatty acid species and monoacylglycerols. Notably, these 18-carbon acyl chains were also constituents of several increased diacylglycerol species. In addition, a number of short- and long-chain acylcarnitines were found to be accumulated while several amino acids were depleted. This study presents unique regional metabolic data indicating a dysregulated energy metabolism in renal mitochondria as an early response to streptozotocin-induced type I diabetes.

National Category
Analytical Chemistry
Research subject
Chemistry with specialization in Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-347672 (URN)10.1007/s00216-019-01721-5 (DOI)000468133600008 ()30895347 (PubMedID)
Funder
Swedish Foundation for Strategic Research Swedish Research CouncilSwedish Diabetes AssociationAstraZeneca
Note

Title in dissertation list of papers: Metabolite aberrations at early onset of diabetes detected in rat kidney using mass spectrometry imaging

Available from: 2018-04-05 Created: 2018-04-05 Last updated: 2020-03-24Bibliographically approved
Duncan, K. D. & Lanekoff, I. (2019). Spatially Defined Surface Sampling Capillary Electrophoresis Mass Spectrometry. Analytical Chemistry, 91(12), 7819-7827
Open this publication in new window or tab >>Spatially Defined Surface Sampling Capillary Electrophoresis Mass Spectrometry
2019 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 91, no 12, p. 7819-7827Article in journal (Refereed) Published
Abstract [en]

Capillary electrophoresis mass spectrometry (CE-MS) is an established technique for targeted and untargeted analysis of metabolites from complex biological samples. However, current CE-MS devices rely on liquid sample extracts, which restricts acquisition of spatially defined chemical information from tissue samples. The ability to chemically profile distinct cellular regions in tissue can contribute better understanding to molecular foundations in health and disease. Therefore, we describe the first CE-MS device capable of untargeted metabolite profiling directly from defined morphological regions of solid tissue sections. With surface sampling capillary electrophoresis mass spectrometry (SS-CE-MS), endogenous molecules are sampled and detected from a single defined tissue location. Characterization of SS-CE MS from different locations of the outer epidermal layer of A. Cepa demonstrated reproducible relative migration times and a peak area RSD of 20% (n = 5). Further, relative migration times were conserved for endogenous metabolites in tissues with varying complexities, including brain, spinal cord, and kidney. Results from proof-of-principle experiments from distinct morphological tissue regions reveal simultaneous analysis of small and large biomolecules, confident metabolite annotation, identification of in-source fragmentation interferences, and discrete isomeric abundances related to biological function. We envision that this new tool will provide in-depth chemical profiling and annotation of molecules in distinct cellular regions of tissue for improved biological understanding.

Place, publisher, year, edition, pages
American Institute of Chemical Engineers, 2019
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-390685 (URN)10.1021/acs.analchem.9b01516 (DOI)000472682000043 ()31124661 (PubMedID)
Funder
Swedish Foundation for Strategic Research , SSF ICA-6Swedish Research Council, VR 621-2013-4231
Available from: 2019-08-14 Created: 2019-08-14 Last updated: 2019-08-14Bibliographically approved
Bemis, K. A., Guo, D., Harry, A. J., Thomas, M., Lanekoff, I., Stenzel-Poore, M. P., . . . Vitek, O. (2019). Statistical detection of differentially abundant ions in mass spectrometry-based imaging experiments with complex designs. International Journal of Mass Spectrometry, 437, 49-57
Open this publication in new window or tab >>Statistical detection of differentially abundant ions in mass spectrometry-based imaging experiments with complex designs
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2019 (English)In: International Journal of Mass Spectrometry, ISSN 1387-3806, E-ISSN 1873-2798, Vol. 437, p. 49-57Article in journal (Refereed) Published
Abstract [en]

Mass Spectrometry Imaging (MSI) characterizes changes in chemical composition between regions of biological samples such as tissues. One goal of statistical analysis of MSI experiments is class comparison, i.e. determining analytes that change in abundance between conditions more systematically than as expected by random variation. To reach accurate and reproducible conclusions, statistical analysis must appropriately reflect the initial research question, the design of the MSI experiment, and all the associated sources of variation. This manuscript highlights the importance of following these general statistical principles. Using the example of two case studies with complex experimental designs, and with different strategies of data acquisition, we demonstrate the extent to which choices made at key points of this workflow impact the results, and provide suggestions for appropriate design and analysis of MSI experiments that aim at detecting differentially abundant analytes. (C) 2018 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV, 2019
Keywords
Mass spectrometry imaging, Nano-DESI MSI, DESI MSI, Experimental design, Statistical analysis, Spatial statistics
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-377202 (URN)10.1016/j.ijms.2018.07.006 (DOI)000456800900008 ()
Funder
Swedish Research CouncilSwedish Foundation for Strategic Research
Available from: 2019-02-25 Created: 2019-02-25 Last updated: 2019-02-25Bibliographically approved
Carter, S.-S., Atif, A. R., Lanekoff, I., Tenje, M. & Mestres, G. (2018). Improving the biocompatibility of PDMS by improving its curing time and temperature. In: : . Paper presented at EUROoC (organ on a chip), 24-25 May 2018, Stuttgart, Germany.
Open this publication in new window or tab >>Improving the biocompatibility of PDMS by improving its curing time and temperature
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2018 (English)Conference paper, Poster (with or without abstract) (Other academic)
Keywords
Organ-on-chip, Biocompatibility, PDMS
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-353156 (URN)
Conference
EUROoC (organ on a chip), 24-25 May 2018, Stuttgart, Germany
Funder
Swedish Research Council Formas, 2016-00781Knut and Alice Wallenberg Foundation, WAF 2016-0112Swedish Research Council, 2017-05051
Available from: 2018-06-11 Created: 2018-06-11 Last updated: 2018-12-11
Duncan, K. D. & Andersson, I. (2018). Oversampling To Improve Spatial Resolution for Liquid Extraction Mass Spectrometry Imaging. Analytical Chemistry, 90(4), 2451-2455
Open this publication in new window or tab >>Oversampling To Improve Spatial Resolution for Liquid Extraction Mass Spectrometry Imaging
2018 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 90, no 4, p. 2451-2455Article in journal (Refereed) Published
Abstract [en]

Liquid extraction mass spectrometry imaging (MSI) experiments provide users with direct analysis of biological surfaces with minimal sample preparation. Until now, much of the effort to increase spatial resolution for MSI with liquid extraction techniques has focused on reducing the size of the sampling area. However, this can be experimentally challenging. Here, we present oversampling as a simple alternative to increase the spatial resolution using nanospray desorption electrospray ionization (nano-DESI) MSI. By imaging partial rat spinal cord tissue sections, two major concerns with oversampling are addressed: whether endogenous molecules are significantly depleted from repeated sampling events and whether analytes are redistributed as a result of oversampling. In depth examination of ion images for representative analytes show that depletion and redistribution do not affect analyte localization in the tissue sample. Nano-DESI MSI experiments using three times oversampling provided higher spatial resolution, allowing the observation of features not visible with undersampling. Although proper care must be taken to ensure that oversampling will work in specific applications, we envision oversampling as a simple approach to increase image quality for liquid extraction MSI techniques.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2018
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-348995 (URN)10.1021/acs.analchem.7b04687 (DOI)000426143100011 ()29373011 (PubMedID)
Funder
Swedish Research Council, VR 621-2013-4231Swedish Foundation for Strategic Research , SSF ICA-6
Available from: 2018-04-19 Created: 2018-04-19 Last updated: 2018-11-29
Lanekoff, I. & Laskin, J. (2018). Quantitative Mass Spectrometry Imaging of Molecules in Biological Systems. In: Grushka, E; Grinberg, N (Ed.), Advances In Chromatography, Vol 54: (pp. 43-72). Boca Raton: CRC Press
Open this publication in new window or tab >>Quantitative Mass Spectrometry Imaging of Molecules in Biological Systems
2018 (English)In: Advances In Chromatography, Vol 54 / [ed] Grushka, E; Grinberg, N, Boca Raton: CRC Press, 2018, p. 43-72Chapter in book (Other academic)
Place, publisher, year, edition, pages
Boca Raton: CRC Press, 2018
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-367260 (URN)10.1201/9781315116372-3 (DOI)000435789000002 ()978-1-138-05595-7 (ISBN)
Available from: 2018-11-29 Created: 2018-11-29 Last updated: 2018-12-12Bibliographically approved
Duncan, K. D., Fang, R., Yuan, J., Chu, R. K., Dey, S. K., Burnum-Johnson, K. E. & Lanekoff, I. (2018). Quantitative Mass Spectrometry Imaging of Prostaglandins as Silver Ion Adducts with Nanospray Desorption Electrospray Ionization. Analytical Chemistry, 90(12), 7246-7252
Open this publication in new window or tab >>Quantitative Mass Spectrometry Imaging of Prostaglandins as Silver Ion Adducts with Nanospray Desorption Electrospray Ionization
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2018 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 90, no 12, p. 7246-7252Article in journal (Refereed) Published
Abstract [en]

Prostaglandins (PG) are an important class of lipid biomolecules that are essential in many biological processes, including inflammation and successful pregnancy. Despite a high bioactivity, physiological concentrations are typically low, which makes direct mass spectrometric analysis of endogenous PG species challenging. Consequently, there have not been any studies investigating PG localization to specific morphological regions in tissue sections using mass spectrometry imaging (MSI) techniques. Herein, we show that silver ions, added to the solvent used for nanospray desorption electrospray ionization (nano-DESI) MSI, enhances the ionization of PGs and enables nano-DESI MSI of several species in uterine tissue from day 4 pregnant mice. It was found that detection of [PG + Ag](+) ions increased the sensitivity by similar to 30 times, when compared to [PG - H](-) ions. Further, the addition of isotopically labeled internal standards enabled generation of quantitative ion images for the detected PG species. Increased sensitivity and quantitative MSI enabled the first proof-of-principle results detailing PG localization in mouse uterus tissue sections. These results show that PG species primarily localized to cellular regions of the luminal epithelium and glandular epithelium in uterine tissue. Further, this study provides a unique scaffold for future studies investigating the PG distribution within biological tissue samples.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2018
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-358686 (URN)10.1021/acs.analchem.8b00350 (DOI)000436028800024 ()29676905 (PubMedID)
Funder
Swedish Foundation for Strategic Research , SSF ICA-6Swedish Research Council, VR 621-2013-4231
Available from: 2018-09-05 Created: 2018-09-05 Last updated: 2018-11-29
Bergman, H.-M., Duncan, K. D. & Lanekoff, I. (2018). Single‐Cell Mass Spectrometry. In: Editor-in-Chief Robert A. Meyers (Ed.), Encyclopedia of Analytical Chemistry: Applications, Theory and Instrumentation. Wiley-VCH Verlagsgesellschaft
Open this publication in new window or tab >>Single‐Cell Mass Spectrometry
2018 (English)In: Encyclopedia of Analytical Chemistry: Applications, Theory and Instrumentation / [ed] Editor-in-Chief Robert A. Meyers, Wiley-VCH Verlagsgesellschaft, 2018Chapter in book (Refereed)
Abstract [en]

Over the past few decades, the chemical characterization of single cells has improved immensely. In particular, mass spectrometry (MS) has pioneered direct analysis of metabolites, lipids, and peptides from single cells. This progress has been enabled by new and improved strategies for ionization and sampling, where a multitude of techniques for single‐cell MS has contributed unique insights to many different disciplines. Here, an overview of the main three techniques secondary ion mass spectrometry (SIMS), matrix‐assisted laser desorption ionization (MALDI), and ambient ionization for direct single‐cell MS analysis are presented, including some example studies detailing the use of single‐cell MS.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2018
National Category
Analytical Chemistry
Research subject
Chemistry with specialization in Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-367252 (URN)10.1002/9780470027318.a9580 (DOI)9780470027318 (ISBN)
Available from: 2018-11-29 Created: 2018-11-29 Last updated: 2018-11-30Bibliographically approved
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ORCID iD: ORCID iD iconorcid.org/0000-0001-9040-3230

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