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Publications (10 of 44) Show all publications
Fornell, A., Ohlin, M., Nilsson, J. & Tenje, M. (2017). A droplet unit operator for controlled particle switching and enrichment. In: : . Paper presented at Micro and Nanofluidics fundamentals and applications (Flow 17), Paris, France, July 3-5 2017. .
Open this publication in new window or tab >>A droplet unit operator for controlled particle switching and enrichment
2017 (English)Conference paper, Oral presentation with published abstract (Refereed)
National Category
Engineering and Technology Medical Laboratory and Measurements Technologies
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-335796 (URN)
Conference
Micro and Nanofluidics fundamentals and applications (Flow 17), Paris, France, July 3-5 2017
Funder
Swedish Research CouncilThe Crafoord Foundation
Available from: 2017-12-11 Created: 2017-12-11 Last updated: 2017-12-29Bibliographically approved
Porras, A. M., Sjögren, F., Shi, L., Ossipov, D. A. & Tenje, M. (2017). Addressing the biocompatibility of photo-crosslinkable hyaluronic acid hydrogels. In: Abstract book at EMBEC 2017 & NBC 2017: . Paper presented at European Medical and Biological Engineering Conference (EMBEC ’17) co-organised with Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC ’17), Tampere, Finland, June 11-15 2017 (pp. 117-117). .
Open this publication in new window or tab >>Addressing the biocompatibility of photo-crosslinkable hyaluronic acid hydrogels
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2017 (English)In: Abstract book at EMBEC 2017 & NBC 2017, 2017, p. 117-117Conference paper, Oral presentation with published abstract (Refereed)
National Category
Engineering and Technology Other Physics Topics
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-335797 (URN)
Conference
European Medical and Biological Engineering Conference (EMBEC ’17) co-organised with Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC ’17), Tampere, Finland, June 11-15 2017
Funder
Swedish Research Council FormasKnut and Alice Wallenberg Foundation
Available from: 2017-12-11 Created: 2017-12-11 Last updated: 2017-12-29Bibliographically approved
Fornell, A., Ohlin, M., Garofalo, F., Nilsson, J. & Tenje, M. (2017). An intra-droplet particle switch for droplet microfluidics using bulk acoustic waves. Biomicrofluidics, 11, Article ID 031101.
Open this publication in new window or tab >>An intra-droplet particle switch for droplet microfluidics using bulk acoustic waves
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2017 (English)In: Biomicrofluidics, ISSN 1932-1058, E-ISSN 1932-1058, Vol. 11, article id 031101Article in journal (Refereed) Published
Abstract [en]

To transfer cell- and bead-assays into droplet-based platforms typically requires the use of complex microfluidic circuits, which calls for methods to switch the direction of the encapsulated particles. We present a microfluidic chip where the combination of acoustic manipulation at two different harmonics and a trident-shaped droplet-splitter enables direction-switching of microbeads and yeast cells in droplet microfluidic circuits. At the first harmonic, the encapsulated particles exit the splitter in the center daughter droplets, while at the second harmonic, the particles exit in the side daughter droplets. This method holds promises for droplet-based assays where particle-positioning needs to be selectively controlled.

National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-324306 (URN)10.1063/1.4984131 (DOI)
Funder
Knut and Alice Wallenberg Foundation, 2012.0023Swedish Research Council, 621-2013-5920The Crafoord Foundation, 20130569
Available from: 2017-06-14 Created: 2017-06-14 Last updated: 2017-06-14Bibliographically approved
Fornell, A., Ohlin, M., Nilsson, J. & Tenje, M. (2017). An optimized droplet split designed for acoustic intra-droplet particle enrichment. In: : . Paper presented at 43rd International Conference on Micro and Nano Engineering (MNE 2017), Braga, Portugal, September 18-22 2017. .
Open this publication in new window or tab >>An optimized droplet split designed for acoustic intra-droplet particle enrichment
2017 (English)Conference paper, Oral presentation with published abstract (Refereed)
Keyword
Acoustics, Droplet microfluidics, Microfabrication
National Category
Engineering and Technology Medical Laboratory and Measurements Technologies
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-335784 (URN)
Conference
43rd International Conference on Micro and Nano Engineering (MNE 2017), Braga, Portugal, September 18-22 2017
Funder
Swedish Research CouncilThe Crafoord Foundation
Available from: 2017-12-11 Created: 2017-12-11 Last updated: 2017-12-29Bibliographically approved
Wolff, A., Thomée, E., Wallman, L. & Tenje, M. (2017). Assembly of a microfluidic device using only plasma activation. In: : . Paper presented at 28th Micromechanics and Microsystems Europe workshop (MME 2017), Uppsala, Sweden, August 23-25 2017. .
Open this publication in new window or tab >>Assembly of a microfluidic device using only plasma activation
2017 (English)Conference paper, Poster (with or without abstract) (Refereed)
Abstract [en]

The blood-brain barrier (BBB) is a complex system, and essential for normal brainfunction as it protects the brain from harmful substances and changes in the blood composition.To analyse the BBB, the simplest way is by using in vitro models, and by utilising microfluidicdevices it is possible to integrate several parameters and analysis steps into one device. We havedeveloped a stable and reproducible bonding protocol for assembling a PDMS-based microfluidicdevice using only plasma activation, to remove the dimensional uncertainty of using intermediatelayer adhesives.

National Category
Engineering and Technology Medical Laboratory and Measurements Technologies
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-335788 (URN)
Conference
28th Micromechanics and Microsystems Europe workshop (MME 2017), Uppsala, Sweden, August 23-25 2017
Funder
Swedish Research Council Formas
Available from: 2017-12-11 Created: 2017-12-11 Last updated: 2017-12-29Bibliographically approved
Andersson, M., Jia, Q., Abella, A., Lee, X.-Y., Landreh, M., Purhonen, P., . . . Rising, A. (2017). Biomimetic spinning of artificial spider silk from a chimeric minispidroin. Nature Chemical Biology, 13(3), 262-264.
Open this publication in new window or tab >>Biomimetic spinning of artificial spider silk from a chimeric minispidroin
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2017 (English)In: Nature Chemical Biology, ISSN 1552-4450, E-ISSN 1552-4469, Vol. 13, no 3, p. 262-264Article in journal (Refereed) Published
Abstract [en]

Herein we present a chimeric recombinant spider silk protein (spidroin) whose aqueous solubility equals that of native spider silk dope and a spinning device that is based solely on aqueous buffers, shear forces and lowered pH. The process recapitulates the complex molecular mechanisms that dictate native spider silk spinning and is highly efficient; spidroin from one liter of bacterial shake-flask culture is enough to spin a kilometer of the hitherto toughest as-spun artificial spider silk fiber.

National Category
Biomaterials Science Materials Engineering
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-316173 (URN)10.1038/nchembio.2269 (DOI)000394431500006 ()28068309 (PubMedID)
Funder
Swedish Research Council, 2014-2408, 2014-10371Swedish Research Council Formas, 2015-629
Available from: 2017-02-25 Created: 2017-02-25 Last updated: 2017-11-29Bibliographically approved
Jocic, S., Mestres, G. & Tenje, M. (2017). Fabrication of user-friendly and biomimetic 1,1′-carbonyldiimidazole cross-linked gelatin/agar microfluidic devices. Materials science & engineering. C, biomimetic materials, sensors and systems, 76, 1175-1180.
Open this publication in new window or tab >>Fabrication of user-friendly and biomimetic 1,1′-carbonyldiimidazole cross-linked gelatin/agar microfluidic devices
2017 (English)In: Materials science & engineering. C, biomimetic materials, sensors and systems, ISSN 0928-4931, E-ISSN 1873-0191, Vol. 76, p. 1175-1180Article in journal (Refereed) Published
Abstract [en]

We have developed a straightforward technique for fabricating user-friendly and biomimetic microfluidic devices out of a gelatin/agar gel cross-linked with 1,1′-carbonyldiimidazole. The fabrication procedure requires only inexpensive starting materials such as glass capillaries and wires to mold 3D cylindrical channels into the gel with the possibility of achieving channel diameters of 375 μm and 1000 μm. We demonstrate that the channel absent of gel injury can retain fluid within its dimensions for at least 7 h. We also show that the device material does not autofluoresce nor provide hindrances with fluorescent imaging. A discussion of the chemical linkage identities of cross-linked gelatin/agar is included via ATR-FTIR studies. Crosslinking of the gelatin/agar is further confirmed by the lack of a gel to sol transition at physiological temperature as assessed by DSC measurements. SEM micrographs that demonstrate the 100 nm mean pore width of the cross-linked gelatin/agar are provided. This device is considered biomimetic because it represents components present in the natural extracellular matrix such as collagen and proteoglycans in the form of cross-linked gelatin/agar.

Keyword
Gelatin; Agar; 1, 1′-carbonyldiimidazole; Biomicrofluidics; ATR-FTIR
National Category
Engineering and Technology Bio Materials
Identifiers
urn:nbn:se:uu:diva-325868 (URN)10.1016/j.msec.2017.03.030 (DOI)000405541600134 ()28482483 (PubMedID)
Funder
Swedish Research Council Formas, 216-2014-1247
Available from: 2017-06-28 Created: 2017-06-28 Last updated: 2017-10-27Bibliographically approved
Ohlin, M., Fornell, A., Bruus, H. & Tenje, M. (2017). Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis. Journal of Micromechanics and Microengineering, 27(8), Article ID 084002.
Open this publication in new window or tab >>Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis
2017 (English)In: Journal of Micromechanics and Microengineering, ISSN 0960-1317, E-ISSN 1361-6439, Vol. 27, no 8, article id 084002Article in journal (Refereed) Published
Abstract [en]

We have fabricated a silicon-glass two-phase droplet microfluidic system capable of generating sub 100 µm-sized,   =  (74  ±  2) µm, spherical droplets at rates of up to hundreds of hertz. By implementing a two-dimensional (2D) acoustophoresis particle-positioning method, we show a fourfold improvement in both vertical and lateral particle positioning inside the droplets compared to unactuated operation. The efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37 °C). Furthermore, by using acoustic actuation, (99.8  ±  0.4)% of all encapsulated microparticles can be detected compared to only (79.0  ±  5.1)% for unactuated operation. In our experiments we observed a strong ordering of the microparticles in distinct patterns within the droplet when using 2D acoustophoresis; to explain the origin of these patterns we simulated numerically the fluid flow inside the droplets and compared with the experimental findings.

National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-327204 (URN)10.1088/1361-6439/aa7967 (DOI)
Funder
The Crafoord Foundation, 20130569Carl Tryggers foundation , CTS14478Swedish Research Council, 612-2013-5920
Available from: 2017-08-07 Created: 2017-08-07 Last updated: 2017-08-07Bibliographically approved
Ohlin, M., Andersson, M., Klintberg, L., Hjort, K. & Tenje, M. (2017). In situ temperature monitoring during acoustophoresis using integrated thin film Pt temperature sensors. In: : . Paper presented at Acoustofluidics 2017, San Diego, USA, August 28-29 2017. .
Open this publication in new window or tab >>In situ temperature monitoring during acoustophoresis using integrated thin film Pt temperature sensors
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2017 (English)Conference paper, Poster (with or without abstract) (Refereed)
National Category
Engineering and Technology Medical Laboratory and Measurements Technologies
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-335786 (URN)
Conference
Acoustofluidics 2017, San Diego, USA, August 28-29 2017
Funder
Swedish Research CouncilThe Crafoord FoundationKnut and Alice Wallenberg Foundation
Available from: 2017-12-11 Created: 2017-12-11 Last updated: 2017-12-29Bibliographically approved
Ohlin, M., Andersson, M., Klintberg, L., Hjort, K. & Tenje, M. (2017). Internal temperature sensing in an acoustophoretic glass chip. In: : . Paper presented at 28th Micromechanics and Microsystems Europe workshop (MME 2017), Uppsala, Sweden, August 23-25 2017. .
Open this publication in new window or tab >>Internal temperature sensing in an acoustophoretic glass chip
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2017 (English)Conference paper, Poster (with or without abstract) (Refereed)
National Category
Engineering and Technology Medical Laboratory and Measurements Technologies
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-335727 (URN)
Conference
28th Micromechanics and Microsystems Europe workshop (MME 2017), Uppsala, Sweden, August 23-25 2017
Available from: 2017-12-07 Created: 2017-12-07 Last updated: 2017-12-29Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-1264-1337

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