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Fornell, A., Cushing, K., Nilsson, J. & Tenje, M. (2018). Binary particle separation in droplet microfluidics using acoustophoresis. Applied Physics Letters, 112(6), Article ID 063701.
Open this publication in new window or tab >>Binary particle separation in droplet microfluidics using acoustophoresis
2018 (English)In: Applied Physics Letters, ISSN 0003-6951, E-ISSN 1077-3118, Vol. 112, no 6, article id 063701Article in journal (Refereed) Published
Abstract [en]

We show a method for separation of two particle species with different acoustic contrasts originally encapsulated in the same droplet in a continuous two-phase system. This was realized by using bulk acoustic standing waves in a 380 mu m wide silicon-glass microfluidic channel. Polystyrene particles (positive acoustic contrast particles) and in-house synthesized polydimethylsiloxane (PDMS) particles (negative acoustic contrast particles) were encapsulated inside water-in-oil droplets either individually or in a mixture. At acoustic actuation of the system at the fundamental resonance frequency, the polystyrene particles were moved to the center of the droplet (pressure node), while the PDMS particles were moved to the sides of the droplet (pressure anti-nodes). The acoustic particle manipulation step was combined in series with a trifurcation droplet splitter, and as the original droplet passed through the splitter and was divided into three daughter droplets, the polystyrene particles were directed into the center daughter droplet, while the PDMS particles were directed into the two side daughter droplets. The presented method expands the droplet microfluidics tool-box and offers new possibilities to perform binary particle separation in droplet microfluidic systems.

National Category
Physical Sciences Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-347079 (URN)10.1063/1.5020356 (DOI)000424703200040 ()
Funder
Swedish Research CouncilThe Crafoord FoundationStiftelsen Olle Engkvist Byggmästare
Available from: 2018-03-26 Created: 2018-03-26 Last updated: 2018-04-04Bibliographically approved
Carter, S.-S., Atif, A. R., Lanekoff, I., Tenje, M. & Mestres, G. (2018). Improving the biocompatibility of PDMS by improving its curing time and temperature. In: : . Paper presented at EUROoC (organ on a chip).
Open this publication in new window or tab >>Improving the biocompatibility of PDMS by improving its curing time and temperature
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2018 (English)Conference paper, Poster (with or without abstract) (Other academic)
National Category
Medical Materials
Identifiers
urn:nbn:se:uu:diva-353156 (URN)
Conference
EUROoC (organ on a chip)
Available from: 2018-06-11 Created: 2018-06-11 Last updated: 2018-06-19
Fornell, A., Garofalo, F., Nilsson, J., Bruus, H. & Tenje, M. (2018). Intra-droplet acoustic particle focusing: simulations and experimental observations. Microfluidics and Nanofluidics, 22(75)
Open this publication in new window or tab >>Intra-droplet acoustic particle focusing: simulations and experimental observations
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2018 (English)In: Microfluidics and Nanofluidics, ISSN 1613-4982, E-ISSN 1613-4990, Vol. 22, no 75Article in journal (Refereed) Published
Abstract [en]

The aim of this paper is to study resonance conditions for acoustic particle focusing inside droplets in two-phase microfluidic systems. A bulk acoustic wave microfluidic chip was designed and fabricated for focusing microparticles inside aqueous droplets (plugs) surrounded by a continuous oil phase in a 380-μm-wide channel. The quality of the acoustic particle focusing was investigated by considering the influence of the acoustic properties of the continuous phase in relation to the dispersed phase. To simulate the system and study the acoustic radiation force on the particles inside droplets, a simplified 3D model was used. The resonance conditions and focusing quality were studied for two different cases: (1) the dispersed and continuous phases were acoustically mismatched (water droplets in fluorinated oil) and (2) the dispersed and continuous phases were acoustically matched (water droplets in olive oil). Experimentally, we observed poor acoustic particle focusing inside droplets surrounded by fluorinated oil while good focusing was observed in droplets surrounded by olive oil. The experimental results are supported qualitatively by our simulations. These show that the acoustic properties (density and compressibility) of the dispersed and continuous phases must be matched to generate a strong and homogeneous acoustic field inside the droplet that is suitable for high-quality intra-droplet acoustic particle focusing.

Place, publisher, year, edition, pages
Springer Berlin/Heidelberg, 2018
Keywords
Acoustophoresis, Droplets, Particle manipulation, Two-phase microfluidics, Ultrasound
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-356418 (URN)10.1007/s10404-018-2094-9 (DOI)
Funder
Swedish Research CouncilThe Crafoord FoundationKnut and Alice Wallenberg Foundation, KAW 2012.0023Stiftelsen Olle Engkvist ByggmästareStiftelsen Längmanska kulturfonden
Available from: 2018-07-26 Created: 2018-07-26 Last updated: 2018-08-01Bibliographically approved
Tenje, M., Fornell, A., Ohlin, M. & Nilsson, J. (2018). Particle Manipulation Methods in Droplet Microfluidics. Analytical Chemistry, 90(3), 1434-1443
Open this publication in new window or tab >>Particle Manipulation Methods in Droplet Microfluidics
2018 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 90, no 3, p. 1434-1443Article in journal (Refereed) Published
Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2018
National Category
Analytical Chemistry Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-348921 (URN)10.1021/acs.analchem.7b01333 (DOI)000424730600002 ()29188994 (PubMedID)
Funder
Swedish Research Council, 621-2013-5920The Crafoord Foundation, 20130569
Available from: 2018-04-25 Created: 2018-04-25 Last updated: 2018-05-02Bibliographically approved
Carter, S.-S., Atif, A., Lanekoff, I., Tenje, M. & Mestres, G. (2018). Tailoring the biocompatibility of the elastomer PDMS for on-chip applications. In: : . Paper presented at Scandinavian Society for Biomaterials.
Open this publication in new window or tab >>Tailoring the biocompatibility of the elastomer PDMS for on-chip applications
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2018 (English)Conference paper, Poster (with or without abstract) (Refereed)
National Category
Medical Materials
Identifiers
urn:nbn:se:uu:diva-353152 (URN)
Conference
Scandinavian Society for Biomaterials
Funder
Swedish Research Council Formas, 2016-00781Swedish Research Council, 2017-05051Knut and Alice Wallenberg Foundation, WAF 2016-0112
Available from: 2018-06-11 Created: 2018-06-11 Last updated: 2018-06-19
Carter, S.-S., Cruz, J., Tenje, M. & Mestres, G. (2018). Towards the development of a microfluidic tool to assess the biological properties of biomaterials for bone regeneration. In: : . Paper presented at EUROoC (organ on a chip).
Open this publication in new window or tab >>Towards the development of a microfluidic tool to assess the biological properties of biomaterials for bone regeneration
2018 (English)Conference paper, Poster (with or without abstract) (Refereed)
National Category
Medical Materials
Identifiers
urn:nbn:se:uu:diva-353155 (URN)
Conference
EUROoC (organ on a chip)
Available from: 2018-06-11 Created: 2018-06-11 Last updated: 2018-06-15Bibliographically approved
Fornell, A., Ohlin, M., Nilsson, J. & Tenje, M. (2017). A droplet unit operator for controlled particle switching and enrichment. In: : . Paper presented at Micro and Nanofluidics fundamentals and applications (Flow 17), Paris, France, July 3-5 2017.
Open this publication in new window or tab >>A droplet unit operator for controlled particle switching and enrichment
2017 (English)Conference paper, Oral presentation with published abstract (Refereed)
National Category
Engineering and Technology Medical Laboratory and Measurements Technologies
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-335796 (URN)
Conference
Micro and Nanofluidics fundamentals and applications (Flow 17), Paris, France, July 3-5 2017
Funder
Swedish Research CouncilThe Crafoord Foundation
Available from: 2017-12-11 Created: 2017-12-11 Last updated: 2017-12-29Bibliographically approved
Porras, A. M., Sjögren, F., Shi, L., Ossipov, D. A. & Tenje, M. (2017). Addressing the biocompatibility of photo-crosslinkable hyaluronic acid hydrogels. In: Abstract book at EMBEC 2017 & NBC 2017: . Paper presented at European Medical and Biological Engineering Conference (EMBEC ’17) co-organised with Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC ’17), Tampere, Finland, June 11-15 2017 (pp. 117-117).
Open this publication in new window or tab >>Addressing the biocompatibility of photo-crosslinkable hyaluronic acid hydrogels
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2017 (English)In: Abstract book at EMBEC 2017 & NBC 2017, 2017, p. 117-117Conference paper, Oral presentation with published abstract (Refereed)
National Category
Engineering and Technology Other Physics Topics
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-335797 (URN)
Conference
European Medical and Biological Engineering Conference (EMBEC ’17) co-organised with Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC ’17), Tampere, Finland, June 11-15 2017
Funder
Swedish Research Council FormasKnut and Alice Wallenberg Foundation
Available from: 2017-12-11 Created: 2017-12-11 Last updated: 2017-12-29Bibliographically approved
Fornell, A., Ohlin, M., Garofalo, F., Nilsson, J. & Tenje, M. (2017). An intra-droplet particle switch for droplet microfluidics using bulk acoustic waves. Biomicrofluidics, 11, Article ID 031101.
Open this publication in new window or tab >>An intra-droplet particle switch for droplet microfluidics using bulk acoustic waves
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2017 (English)In: Biomicrofluidics, ISSN 1932-1058, E-ISSN 1932-1058, Vol. 11, article id 031101Article in journal (Refereed) Published
Abstract [en]

To transfer cell- and bead-assays into droplet-based platforms typically requires the use of complex microfluidic circuits, which calls for methods to switch the direction of the encapsulated particles. We present a microfluidic chip where the combination of acoustic manipulation at two different harmonics and a trident-shaped droplet-splitter enables direction-switching of microbeads and yeast cells in droplet microfluidic circuits. At the first harmonic, the encapsulated particles exit the splitter in the center daughter droplets, while at the second harmonic, the particles exit in the side daughter droplets. This method holds promises for droplet-based assays where particle-positioning needs to be selectively controlled.

National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-324306 (URN)10.1063/1.4984131 (DOI)
Funder
Knut and Alice Wallenberg Foundation, 2012.0023Swedish Research Council, 621-2013-5920The Crafoord Foundation, 20130569
Available from: 2017-06-14 Created: 2017-06-14 Last updated: 2017-06-14Bibliographically approved
Fornell, A., Ohlin, M., Nilsson, J. & Tenje, M. (2017). An optimized droplet split designed for acoustic intra-droplet particle enrichment. In: : . Paper presented at 43rd International Conference on Micro and Nano Engineering (MNE 2017), Braga, Portugal, September 18-22 2017.
Open this publication in new window or tab >>An optimized droplet split designed for acoustic intra-droplet particle enrichment
2017 (English)Conference paper, Oral presentation with published abstract (Refereed)
Keywords
Acoustics, Droplet microfluidics, Microfabrication
National Category
Engineering and Technology Medical Laboratory and Measurements Technologies
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-335784 (URN)
Conference
43rd International Conference on Micro and Nano Engineering (MNE 2017), Braga, Portugal, September 18-22 2017
Funder
Swedish Research CouncilThe Crafoord Foundation
Available from: 2017-12-11 Created: 2017-12-11 Last updated: 2017-12-29Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-1264-1337

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