uu.seUppsala University Publications
Change search
Link to record
Permanent link

Direct link
BETA
Publications (10 of 59) Show all publications
Cantoni, F., Hilborn, J., Johansson, S., Pohlit, H., Porras, A. M., Samanta, A. & Tenje, M. (2018). 2D and 3D patterning of biological hydrogels for organ-on-chip applications. In: : . Paper presented at 44th International Conference on Micro and Nano Engineering (MNE 2018), Copenhagen, Denmark, September 24-27 2018..
Open this publication in new window or tab >>2D and 3D patterning of biological hydrogels for organ-on-chip applications
Show others...
2018 (English)Conference paper, Oral presentation with published abstract (Other academic)
Keywords
Organs-on-chip, Hydrogels, UV lithography, Microfluidics, TEER
National Category
Engineering and Technology Other Medical Engineering
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-369364 (URN)
Conference
44th International Conference on Micro and Nano Engineering (MNE 2018), Copenhagen, Denmark, September 24-27 2018.
Funder
Knut and Alice Wallenberg Foundation, WAF 2016-0112EU, European Research Council
Available from: 2018-12-13 Created: 2018-12-13 Last updated: 2018-12-13Bibliographically approved
Gonska, N., Robinson, N., Barbe, L., Tenje, M. & Johansson, J. (2018). Artificial spider silk production on a microfluidic chip. In: : . Paper presented at 10th European Solid Mechanics Conference (ESMC 2018), Bologna, Italy, July 2-6 2018.
Open this publication in new window or tab >>Artificial spider silk production on a microfluidic chip
Show others...
2018 (English)Conference paper, Poster (with or without abstract) (Other academic)
Keywords
spider silk, microfluidics
National Category
Engineering and Technology Other Materials Engineering
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-369428 (URN)
Conference
10th European Solid Mechanics Conference (ESMC 2018), Bologna, Italy, July 2-6 2018
Available from: 2018-12-13 Created: 2018-12-13 Last updated: 2018-12-14Bibliographically approved
Fornell, A., Cushing, K., Nilsson, J. & Tenje, M. (2018). Binary particle separation in droplet microfluidics using acoustophoresis. Applied Physics Letters, 112(6), Article ID 063701.
Open this publication in new window or tab >>Binary particle separation in droplet microfluidics using acoustophoresis
2018 (English)In: Applied Physics Letters, ISSN 0003-6951, E-ISSN 1077-3118, Vol. 112, no 6, article id 063701Article in journal (Refereed) Published
Abstract [en]

We show a method for separation of two particle species with different acoustic contrasts originally encapsulated in the same droplet in a continuous two-phase system. This was realized by using bulk acoustic standing waves in a 380 mu m wide silicon-glass microfluidic channel. Polystyrene particles (positive acoustic contrast particles) and in-house synthesized polydimethylsiloxane (PDMS) particles (negative acoustic contrast particles) were encapsulated inside water-in-oil droplets either individually or in a mixture. At acoustic actuation of the system at the fundamental resonance frequency, the polystyrene particles were moved to the center of the droplet (pressure node), while the PDMS particles were moved to the sides of the droplet (pressure anti-nodes). The acoustic particle manipulation step was combined in series with a trifurcation droplet splitter, and as the original droplet passed through the splitter and was divided into three daughter droplets, the polystyrene particles were directed into the center daughter droplet, while the PDMS particles were directed into the two side daughter droplets. The presented method expands the droplet microfluidics tool-box and offers new possibilities to perform binary particle separation in droplet microfluidic systems.

National Category
Engineering and Technology Physical Sciences
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-347079 (URN)10.1063/1.5020356 (DOI)000424703200040 ()
Funder
Swedish Research CouncilThe Crafoord FoundationStiftelsen Olle Engkvist Byggmästare
Available from: 2018-03-26 Created: 2018-03-26 Last updated: 2018-12-06Bibliographically approved
Atif, A. R., Carter, S.-S., Pujari-Palmer, M., Tenje, M. & Mestres, G. (2018). Bone Cement Embedded in a Microfluidic Device. In: : . Paper presented at Micronano System Workshop (MSW), May 13-15, 2018, Aalto University, Espoo, Finland.
Open this publication in new window or tab >>Bone Cement Embedded in a Microfluidic Device
Show others...
2018 (English)Conference paper, Poster (with or without abstract) (Refereed)
Abstract [en]

Calcium phosphate cements (CPCs) have a great potential in the treatment of bone disorders due to their excellent biocompatibility. Although CPCs are promising when implanted in vivo, there is poor correlation between in vitro and in vivo studies. This could be because most conventional in vitro systems lack a 3D architecture, or dynamic conditions (i.e. a continuous refreshment stream). The aim of this work is to embed CPCs into a microfluidic system and evaluate ion and protein exchange at different flow rates.

Keywords
Calcium Phosphate Cements, Microfluidic Chip, Continuous flow, Biomaterial Evaluation, Bone implants, Cells
National Category
Other Materials Engineering Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-363447 (URN)
Conference
Micronano System Workshop (MSW), May 13-15, 2018, Aalto University, Espoo, Finland
Funder
Swedish Research Council Formas, 2016-00781Knut and Alice Wallenberg Foundation, WAF 2016-0112Swedish Research Council, 2017-05051
Available from: 2018-10-18 Created: 2018-10-18 Last updated: 2018-12-04Bibliographically approved
Porras, A. M., Shi, L., Ossipov, D. A. & Tenje, M. (2018). Chemical micropatterning of hyaluronic acid hydrogels for controlled cell adhesion. In: : . Paper presented at European Organ on Chip, Stuttgart, Germany 24-25 May, 2018.
Open this publication in new window or tab >>Chemical micropatterning of hyaluronic acid hydrogels for controlled cell adhesion
2018 (English)Conference paper, Poster (with or without abstract) (Other academic)
Abstract [en]

The blood brain barrier is constituted by endothelial cells, astrocytes and pericytes; and are organized into well structured units [1]. Standard cell culture techniques cannot recapitulate this organized structure. Hydrogels are an attractive scaffold due to their mechanical and chemical properties similar to those in body tissue[2] We propose the use of a photo-crosslinkable hyaluronic acid hydrogel as cell culture scaffold. Furthermore, chemical cues can be added into the hydrogel matrix to promote and control cell adhesion using UV lithography.

National Category
Engineering and Technology Other Medical Engineering
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-368272 (URN)
Conference
European Organ on Chip, Stuttgart, Germany 24-25 May, 2018
Funder
Wallenberg Foundations, 2016-0112EU, Horizon 2020, 757444
Available from: 2018-12-05 Created: 2018-12-05 Last updated: 2018-12-11Bibliographically approved
Searle, S., Porras, A. M., Barbe, L., Shi, L., Ossipov, D. A., Trau, D. & Tenje, M. (2018). Hyaluronic acid based hydrogel droplets: A potential injectable cell culture scaffold. In: : . Paper presented at EUROoC, Stuttgart, Germany. 24 and 35 May, 2018..
Open this publication in new window or tab >>Hyaluronic acid based hydrogel droplets: A potential injectable cell culture scaffold
Show others...
2018 (English)Conference paper, Oral presentation only (Other academic)
Abstract [en]

Introduction

Cell culture scaffolds such as hydrogels give support and structure for cultured cells in 3D environments that better mimic in vivo conditions [1]. Hyaluronic acid (HA) derived hydrogels are particularly attractive scaffold materials, due to their high water content, and its high presence in the extracellular matrix of a multitude of tissues in the human body [2]. Adequate diffusion of oxygen and nutrients however, is generally limited to a depth of 200 µm in bulk hydrogels [3], heavily limiting their applicability to relatively large size constructs. We propose the use of droplet-based microfluidics to produce monodisperse HA-derived injectable microgel droplets which could enable the diffusion of nutrients and metabolites, while maintaining a size in which encapsulating sufficient cells to allow cell-cell interactions and proliferation would be possible.

 

Experimental results

Hyaluronic acid acrylamide (HA-am) was synthesized by partially modifying high molecular weight sodium hyaluronan with a N-(2-aminoethyl)acrylamide linker. Degree of modification was confirmed by NMR to be of 20%. HA-am bulk hydrogels were formed by exposing a solution of HA-am and photoinitiator Irgacure 2959 (0.4 % w/v) to a UV light source of 365 nm wavelength. Gel droplets were produced in a PDMS microfluidic device designed in a flow focusing geometry. In order to simulate cell encapsulation in the microgel, hydrogel precursor mixtures were prepared as for bulk hydrogels with the addition of polystyrene beads (10µm in diameter) at a concentration of 10 million beads ml-1. For the oil phase, a fluorinated oil (Novec 7500, 3M) with 0.5% surfactant (PicoSurf 1) was used. The flow rates for the oil phase and aqueous phase were adjusted to 15 and 5 µl min-1, respectively to produce highly monodisperse droplets of 151 µm in average diameter. Collected droplets were polymerized by exposing to UV light, washed and transferred to an aqueous solution.

 

 

Conclusion

Highly monodisperse microgels containing microbeads were obtained. We demonstrate that photocrosslinkable hydrogel droplets can be produced from HA-am in a microfluidic flow-focusing chip which could enable the encapsulation of cells and the use of the droplets as injectable cell culture scaffolds.

 

References

[1]       G. D. Nicodemus and S. J. Bryant, “Cell Encapsulation in Biodegradable Hydrogels for Tissue Engineering Applications,” Tissue Eng. Part B Rev., vol. 14, no. 2, pp. 149–165, Jun. 2008.

[2]       J. A. Burdick and G. D. Prestwich, “Hyaluronic acid hydrogels for biomedical applications,” Adv. Mater., vol. 23, no. 12, pp. 41–56, Mar. 2011.

[3]       H. Huang, Y. Yu, Y. Hu, X. He, O. Berk Usta, and M. L. Yarmush, “Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture,” Lab Chip, vol. 17, no. 11, pp. 1913–1932, 2017.

National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-357613 (URN)
Conference
EUROoC, Stuttgart, Germany. 24 and 35 May, 2018.
Available from: 2018-08-17 Created: 2018-08-17 Last updated: 2018-08-22Bibliographically approved
Carter, S.-S., Atif, A. R., Lanekoff, I., Tenje, M. & Mestres, G. (2018). Improving the biocompatibility of PDMS by improving its curing time and temperature. In: : . Paper presented at EUROoC (organ on a chip), 24-25 May 2018, Stuttgart, Germany.
Open this publication in new window or tab >>Improving the biocompatibility of PDMS by improving its curing time and temperature
Show others...
2018 (English)Conference paper, Poster (with or without abstract) (Other academic)
Keywords
Organ-on-chip, Biocompatibility, PDMS
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-353156 (URN)
Conference
EUROoC (organ on a chip), 24-25 May 2018, Stuttgart, Germany
Funder
Swedish Research Council Formas, 2016-00781Knut and Alice Wallenberg Foundation, WAF 2016-0112Swedish Research Council, 2017-05051
Available from: 2018-06-11 Created: 2018-06-11 Last updated: 2018-12-11
Johansson, S., Porras, A. M., Searle, S., Barbe, L. & Tenje, M. (2018). Integrated transparent electrodes in an organs-on-chip system. In: : . Paper presented at Micronano System Workshop (MSW), Espoo, Finland, May 13th to 18th 2018.
Open this publication in new window or tab >>Integrated transparent electrodes in an organs-on-chip system
Show others...
2018 (English)Conference paper, Oral presentation only (Refereed)
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-363355 (URN)
Conference
Micronano System Workshop (MSW), Espoo, Finland, May 13th to 18th 2018
Funder
EU, Horizon 2020, 757444
Available from: 2018-10-17 Created: 2018-10-17 Last updated: 2018-12-06Bibliographically approved
Fornell, A., Garofalo, F., Nilsson, J., Bruus, H. & Tenje, M. (2018). Intra-droplet acoustic particle focusing: simulations and experimental observations. Microfluidics and Nanofluidics, 22(75)
Open this publication in new window or tab >>Intra-droplet acoustic particle focusing: simulations and experimental observations
Show others...
2018 (English)In: Microfluidics and Nanofluidics, ISSN 1613-4982, E-ISSN 1613-4990, Vol. 22, no 75Article in journal (Refereed) Published
Abstract [en]

The aim of this paper is to study resonance conditions for acoustic particle focusing inside droplets in two-phase microfluidic systems. A bulk acoustic wave microfluidic chip was designed and fabricated for focusing microparticles inside aqueous droplets (plugs) surrounded by a continuous oil phase in a 380-μm-wide channel. The quality of the acoustic particle focusing was investigated by considering the influence of the acoustic properties of the continuous phase in relation to the dispersed phase. To simulate the system and study the acoustic radiation force on the particles inside droplets, a simplified 3D model was used. The resonance conditions and focusing quality were studied for two different cases: (1) the dispersed and continuous phases were acoustically mismatched (water droplets in fluorinated oil) and (2) the dispersed and continuous phases were acoustically matched (water droplets in olive oil). Experimentally, we observed poor acoustic particle focusing inside droplets surrounded by fluorinated oil while good focusing was observed in droplets surrounded by olive oil. The experimental results are supported qualitatively by our simulations. These show that the acoustic properties (density and compressibility) of the dispersed and continuous phases must be matched to generate a strong and homogeneous acoustic field inside the droplet that is suitable for high-quality intra-droplet acoustic particle focusing.

Place, publisher, year, edition, pages
Springer Berlin/Heidelberg, 2018
Keywords
Acoustophoresis, Droplets, Particle manipulation, Two-phase microfluidics, Ultrasound
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-356418 (URN)10.1007/s10404-018-2094-9 (DOI)000436895700001 ()
Funder
Swedish Research CouncilThe Crafoord FoundationKnut and Alice Wallenberg Foundation, KAW 2012.0023Stiftelsen Olle Engkvist ByggmästareStiftelsen Längmanska kulturfondenStiftelsen Olle Engkvist Byggmästare
Available from: 2018-07-26 Created: 2018-07-26 Last updated: 2018-09-06Bibliographically approved
Tenje, M. (2018). Introducing acoustophoresis to droplet microfluidics for particle manipulation. In: : . Paper presented at 12th Micronano System Workshop (MSW 2018), Espoo, Finland, May 13-15 2018.
Open this publication in new window or tab >>Introducing acoustophoresis to droplet microfluidics for particle manipulation
2018 (English)Conference paper, Oral presentation only (Other academic)
National Category
Engineering and Technology Other Physics Topics
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-369366 (URN)
Conference
12th Micronano System Workshop (MSW 2018), Espoo, Finland, May 13-15 2018
Funder
Swedish Research Council
Available from: 2018-12-13 Created: 2018-12-13 Last updated: 2018-12-13Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-1264-1337

Search in DiVA

Show all publications