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Publications (7 of 7) Show all publications
Mutisya, J., Sun, C., Rosenquist, S., Baguma, Y. & Jansson, C. (2009). Diurnal oscillation of SBE expression in sorghum endosperm. Journal of plant physiology (Print), 166(4), 428-434
Open this publication in new window or tab >>Diurnal oscillation of SBE expression in sorghum endosperm
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2009 (English)In: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 166, no 4, p. 428-434Article in journal (Refereed) Published
Abstract [en]

Summary Spatial and temporal expression patterns of the sorghum SBEI, SBEIIA and SBEIIB genes, encoding, respectively, starch branching enzyme (SBE) I, IIA and IIB, in the developing endosperm of sorghum (Sorghum bicolor) were studied. Full-length genomic and cDNA clones for sorghum were cloned, and the SBEIIA cDNA was used together with gene-specific probes for sorghum SBEIIB and SBEI. In contrast to sorghum SBEIIB, which was expressed primarily in endosperm and embryo, SBEIIA was also expressed in vegetative tissues. All three genes shared a similar temporal expression profile during endosperm development, with a maximum activity at 15–24 d after pollination. This differed from barley and maize, in which SBEI gene activity showed a significantly later onset compared to that of SBEIIA and SBEIIB. Expression of the three SBE genes in the sorghum endosperm exhibited a diurnal rhythm during a 24-h cycle.

Keywords
Barley, Endosperm, Oscillation, SBE, Sorghum
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-256638 (URN)10.1016/j.jplph.2008.06.017 (DOI)
Available from: 2015-06-24 Created: 2015-06-24 Last updated: 2017-12-04
Hammargren, J., Rosenquist, S., Jansson, C. & Knorpp, C. (2008). A novel connection between nucleotide and carbohydrate metabolism in mitochondria: sugar regulation of the Arabidopsis nucleoside diphosphate kinase 3a gene. Plant Cell Reports, 27(3), 529-534
Open this publication in new window or tab >>A novel connection between nucleotide and carbohydrate metabolism in mitochondria: sugar regulation of the Arabidopsis nucleoside diphosphate kinase 3a gene
2008 (English)In: Plant Cell Reports, ISSN 0721-7714, E-ISSN 1432-203X, Vol. 27, no 3, p. 529-534Article in journal (Refereed) Published
Keywords
Mitochondria; NDPK; Nucleoside diphosphate kinase; Plant; Sugar signalling; Sucrose; Turanose; WRKY
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-256637 (URN)10.1007/s00299-007-0486-5 (DOI)
Available from: 2015-06-24 Created: 2015-06-24 Last updated: 2017-12-04
Mutisya, J., Sun, C., Palmqvist, S., Baguma, Y., Odhiambo, B. & Jansson, C. (2006). Transcriptional regulation of the sbeIIb genes in sorghum (Sorghum bicolor) and barley (Hordeum vulgare): Importance of the barley sbeIIb second intron. Journal of plant physiology (Print), 163(7), 770-780
Open this publication in new window or tab >>Transcriptional regulation of the sbeIIb genes in sorghum (Sorghum bicolor) and barley (Hordeum vulgare): Importance of the barley sbeIIb second intron
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2006 (English)In: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 163, no 7, p. 770-780Article in journal (Refereed) Published
Abstract [en]

Summary The transcriptional activity of the sorghum sbeIIb gene, encoding starch branching enzyme IIb, is seed specific, with expression in both the endosperm and the embryo. In comparison, expression of barley sbeIIb is confined to the endosperm, whereas that of barley sbeIIa occurs in endosperm, embryonic and vegetative tissues. It has been suggested that the second intron of barley sbeIIb may be instrumental in conferring endosperm-specific expression. Therefore, to further investigate the regulatory mechanisms of barley and sorghum sbe, we examined the tissue-specific activity of the sorghum sbe promoter in transient assays of green fluorescent protein (gfp) reporter constructs. We found that, when linked to the barley sbeIIb second intron, the sorghum sbeIIb promoter could not drive gfp transcription in sorghum or barley embryonic cells. Similar results were obtained for the barley sbeIIa promoter. Database searches showed that sequences homologous to the barley sbeIIb intron also exist in introns and flanking regions of some other grass genes. Deletion mutagenesis of the sorghum sbeIIb promoter identified the minimal promoters required for high- and low-level expression, respectively, but did not reveal any putative promoter elements crucial for expression. A sequence with similarity to the SURE element, implicated in sugar signaling, was located in the distal promoter region of sorghum sbeIIb, upstream of the minimal promoters. SURE elements are present in the proximal promoter regions of the sugar-regulated barley iso1 gene, and barley sbeIIb. In keeping in line with these observations, RNA-gel blot analyses demonstrated that expression of barley sbeIIb was sugar inducible, whereas that of sorghum sbeIIb was not.

Keywords
Barley, Intron, sbeIIb, Sorghum
National Category
Genetics
Identifiers
urn:nbn:se:uu:diva-256606 (URN)10.1016/j.jplph.2005.04.038 (DOI)
Available from: 2015-06-24 Created: 2015-06-24 Last updated: 2017-12-04
Edqvist, J., Rönnberg, E., Rosenquist, S., Blomqvist, K., Viitanen, L., Salminen, T. A., . . . Mattjus, P. (2004). Plants express a lipid transfer protein with high similarity to mammalian sterol carrier protein-2.. Journal of Biological Chemistry, 279(51), 53544-53
Open this publication in new window or tab >>Plants express a lipid transfer protein with high similarity to mammalian sterol carrier protein-2.
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2004 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 279, no 51, p. 53544-53Article in journal (Refereed) Published
Abstract [en]

This is the first report describing the cloning and characterization of sterol carrier protein-2 (SCP-2) from plants. Arabidopsis thaliana SCP-2 (AtSCP-2) consists of 123 amino acids with a molecular mass of 13.6 kDa. AtSCP-2 shows 35% identity and 56% similarity to the human SCP-2-like domain present in the human D-bifunctional protein (DBP) and 30% identity and 54% similarity to the human SCP-2 encoded by SCP-X. The presented structural models of apo-AtSCP-2 and the ligand-bound conformation of AtSCP-2 reveal remarkable similarity with two of the structurally known SCP-2s, the SCP-2-like domain of human DBP and the rabbit SCP-2, correspondingly. The AtSCP-2 models in both forms have a similar hydrophobic ligand-binding tunnel, which is extremely suitable for lipid binding. AtSCP-2 showed in vitro transfer activity of BODIPY-phosphatidylcholine (BODIPY-PC) from donor membranes to acceptor membranes. The transfer of BODIPY-PC was almost completely inhibited after addition of 1-palmitoyl 2-oleoyl phosphatidylcholine or ergosterol. Dimyristoyl phosphatidic acid, stigmasterol, steryl glucoside, and cholesterol showed a moderate to marginal ability to lower the BODIPY-PC transfer rate, and the single chain palmitic acid and stearoyl-coenzyme A did not affect transfer at all. Expression analysis showed that AtSCP-2 mRNA is accumulating in most plant tissues. Plasmids carrying fusion genes between green fluorescent protein and AtSCP-2 were transformed with particle bombardment to onion epidermal cells. The results from analyzing the transformants indicate that AtSCP-2 is localized to peroxisomes.

National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-256639 (URN)10.1074/jbc.M405099200 (DOI)15456765 (PubMedID)
Available from: 2015-06-24 Created: 2015-06-24 Last updated: 2017-12-04
Sun, C., Palmqvist, S., Olsson, H., Borén, M., Ahlandsberg, S. & Jansson, C. (2003). A Novel WRKY Transcription Factor, SUSIBA2, Participates in Sugar Signaling in Barley by Binding to the Sugar-Responsive Elements of the iso1 Promoter. The Plant Cell, 15(9), 2076-2092
Open this publication in new window or tab >>A Novel WRKY Transcription Factor, SUSIBA2, Participates in Sugar Signaling in Barley by Binding to the Sugar-Responsive Elements of the iso1 Promoter
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2003 (English)In: The Plant Cell, Vol. 15, no 9, p. 2076-2092Article in journal (Refereed) Published
Abstract [en]

SURE (sugar responsive) is a cis element in plant sugar signaling. The SURE element was reported first for potato, in which it confers sugar responsiveness to the patatin promoter. A SURE binding transcription factor has not been isolated. We have isolated a transcription factor cDNA from barley and purified the corresponding protein. The transcription factor, SUSIBA2 (sugar signaling in barley), belongs to the WRKY proteins and was shown to bind to SURE and W-box elements but not to the SP8a element in the iso1 promoter. Nuclear localization of SUSIBA2 was demonstrated in a transient assay system with a SUSIBA2:green fluorescent protein fusion protein. Exploiting the novel transcription factor oligodeoxynucleotide decoy strategy with transformed barley endosperm provided experimental evidence for the importance of the SURE elements in iso1 transcription. Antibodies against SUSIBA2 were produced, and the expression pattern for susiba2 was determined at the RNA and protein levels. It was found that susiba2 is expressed in endosperm but not in leaves. Transcription of susiba2 is sugar inducible, and ectopic susiba2 expression was obtained in sugar-treated leaves. Likewise, binding to SURE elements was observed for nuclear extracts from sugar-treated but not from control barley leaves. The temporal expression of susiba2 in barley endosperm followed that of iso1 and endogenous sucrose levels, with a peak at similar to12 days after pollination. Our data indicate that SUSIBA2 binds to the SURE elements in the barley iso1 promoter as an activator. Furthermore, they show that SUSIBA2 is a regulatory transcription factor in starch synthesis and demonstrate the involvement of a WRKY protein in carbohydrate anabolism. Orthologs to SUSIBA2 were isolated from rice and wheat endosperm.; SURE (sugar responsive) is a cis element in plant sugar signaling. The SURE element was reported first for potato, in which it confers sugar responsiveness to the patatin promoter. A SURE binding transcription factor has not been isolated. We have isolated a transcription factor cDNA from barley and purified the corresponding protein. The transcription factor, SUSIBA2 (sugar signaling in barley), belongs to the WRKY proteins and was shown to bind to SURE and W-box elements but not to the SP8a element in the iso1 promoter. Nuclear localization of SUSIBA2 was demonstrated in a transient assay system with a SUSIBA2:green fluorescent protein fusion protein. Exploiting the novel transcription factor oligodeoxynucleotide decoy strategy with transformed barley endosperm provided experimental evidence for the importance of the SURE elements in iso1 transcription. Antibodies against SUSIBA2 were produced, and the expression pattern for susiba2 was determined at the RNA and protein levels. It was found that susiba2 is expressed in endosperm but not in leaves. Transcription of susiba2 is sugar inducible, and ectopic susiba2 expression was obtained in sugar-treated leaves. Likewise, binding to SURE elements was observed for nuclear extracts from sugar-treated but not from control barley leaves. The temporal expression of susiba2 in barley endosperm followed that of iso1 and endogenous sucrose levels, with a peak at approximately 12 days after pollination. Our data indicate that SUSIBA2 binds to the SURE elements in the barley iso1 promoter as an activator. Furthermore, they show that SUSIBA2 is a regulatory transcription factor in starch synthesis and demonstrate the involvement of a WRKY protein in carbohydrate anabolism. Orthologs to SUSIBA2 were isolated from rice and wheat endosperm.; SURE (sugar responsive) is a cis element in plant sugar signaling. The SURE element was reported first for potato, in which it confers sugar responsiveness to the patatin promoter. A SURE binding transcription factor has not been isolated. We have isolated a transcription factor cDNA from barley and purified the corresponding protein. The transcription factor, SUSIBA2 (sugar signaling in barley), belongs to the WRKY proteins and was shown to bind to SURE and W-box elements but not to the SP8a element in the iso1 promoter. Nuclear localization of SUSIBA2 was demonstrated in a transient assay system with a SUSIBA2:green fluorescent protein fusion protein. Exploiting the novel transcription factor oligodeoxynucleotide decoy strategy with transformed barley endosperm provided experimental evidence for the importance of the SURE elements in iso1 transcription. Antibodies against SUSIBA2 were produced, and the expression pattern for susiba2 was determined at the RNA and protein levels. It was found that susiba2 is expressed in endosperm but not in leaves. Transcription of susiba2 is sugar inducible, and ectopic susiba2 expression was obtained in sugar-treated leaves. Likewise, binding to SURE elements was observed for nuclear extracts from sugar-treated but not from control barley leaves. The temporal expression of susiba2 in barley endosperm followed that of iso1 and endogenous sucrose levels, with a peak at similar to 12 days after pollination. Our data indicate that SUSIBA2 binds to the SURE elements in the barley iso1 promoter as an activator. Furthermore, they show that SUSIBA2 is a regulatory transcription factor in starch synthesis and demonstrate the involvement of a WRKY protein in carbohydrate anabolism. Orthologs to SUSIBA2 were isolated from rice and wheat endosperm.; SURE (sugar responsive) is a cis element in plant sugar signaling. The SURE element was reported first for potato, in which it confers sugar responsiveness to the patatin promoter. A SURE binding transcription factor has not been isolated. We have isolated a transcription factor cDNA from barley and purified the corresponding protein. The transcription factor, SUSIBA2 (sugar signaling in barley), belongs to the WRKY proteins and was shown to bind to SURE and W-box elements but not to the SP8a element in the iso1 promoter. Nuclear localization of SUSIBA2 was demonstrated in a transient assay system with a SUSIBA2:green fluorescent protein fusion protein. Exploiting the novel transcription factor oligodeoxynucleotide decoy strategy with transformed barley endosperm provided experimental evidence for the importance of the SURE elements in iso1 transcription. Antibodies against SUSIBA2 were produced, and the expression pattern for susiba2 was determined at the RNA and protein levels. It was found that susiba2 is expressed in endosperm but not in leaves. Transcription of susiba2 is sugar inducible, and ectopic susiba2 expression was obtained in sugar-treated leaves. Likewise, binding to SURE elements was observed for nuclear extracts from sugar-treated but not from control barley leaves. The temporal expression of susiba2 in barley endosperm followed that of iso1 and endogenous sucrose levels, with a peak at ∌12 days after pollination. Our data indicate that SUSIBA2 binds to the SURE elements in the barley iso1 promoter as an activator. Furthermore, they show that SUSIBA2 is a regulatory transcription factor in starch synthesis and demonstrate the involvement of a WRKY protein in carbohydrate anabolism. Orthologs to SUSIBA2 were isolated from rice and wheat endosperm.

Keywords
PLANT SCIENCES; ENDOSPERM; CELL BIOLOGY; GENE-EXPRESSION; PROTEIN; SWEET-POTATO; BETA-AMYLASE; AMYLOPECTIN BIOSYNTHESIS; STARCH-BRANCHING ENZYMES; BIOCHEMISTRY & MOLECULAR BIOLOGY; DNA; PLANTS; ARABIDOPSIS-THALIANA; Hordeum vulgare; Promoters; Carbohydrate metabolism; Barley; cDNA; Transcription factors; Gene regulation; SUSIBA2 gene; Starch; Nuclear Localization Signals - metabolism; Triticum - metabolism; Escherichia coli - genetics; Response Elements - genetics; Hordeum - metabolism; Recombinant Proteins - chemistry; Gene Expression Regulation, Plant - drug effects; DNA-Binding Proteins - genetics; Transcription Factors - genetics; Transcription Factors - metabolism; Signal Transduction - genetics; Carbohydrates - pharmacology; Nuclear Localization Signals - genetics; DNA, Complementary - genetics; Oryza sativa - genetics; Recombinant Proteins - isolation & purification; Promoter Regions, Genetic - genetics; Recombinant Proteins - metabolism; Seeds - genetics; DNA-Binding Proteins - metabolism; Plant Proteins - genetics; Oryza sativa - metabolism; Hordeum - genetics; Plant Proteins - metabolism; DNA, Complementary - chemistry; Sucrose - metabolism; Seeds - metabolism; Triticum - genetics; Research; Plant proteins; Genetic transcription; Physiological aspects
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-256600 (URN)
Available from: 2015-06-24 Created: 2015-06-24 Last updated: 2015-06-24
Baguma, Y., Sun, C., Ahlandsberg, S., Mutisya, J., Palmqvist, S., Rubaihayo, P. R., . . . Jansson, C. (2003). Expression patterns of the gene encoding starch branching enzyme II in the storage roots of cassava (Manihot esculenta Crantz). Plant Science, 164(5), 833-839
Open this publication in new window or tab >>Expression patterns of the gene encoding starch branching enzyme II in the storage roots of cassava (Manihot esculenta Crantz)
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2003 (English)In: Plant Science, ISSN 0168-9452, E-ISSN 1873-2259, Vol. 164, no 5, p. 833-839Article in journal (Refereed) Published
Abstract [en]

Spatial and temporal expression patterns of the sbeII and sbeI genes, encoding starch branching enzyme II and I, respectively, in cassava (Manihot esculenta Crantz) were studied at different phenological stages of the crop. A partial cDNA for sbeII in cassava was cloned and used along with a cDNA-specific fragment of sbeI. As the cassava plant aged, the transcriptional activity of the sbeII and sbeI genes in the underground storage roots increased, whereas the activity in other organs remained the same or declined. At 180 days after planting (d.a.p.), levels of sbeII and sbeI transcripts in storage roots were very low, whereas at 360 d.a.p., the levels had increased dramatically. The 360 d.a.p. old storage roots also accumulated gbssII and gbssI transcripts, as well as a longer gbssI transcript, gbssI′. The difference between the gbssI and gbssI′ transcripts was shown to be due to differential splicing, whereby the gbssI′ transcript retained the first three introns. Unexpectedly, expression of sbeII and sbeI in the 360 d.a.p. storage roots exhibited fluctuations during the 24 h cycle, both under the normal light/dark regime and under continuous light or continuous dark conditions.

Keywords
Cassava, sbe, Starch
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-256620 (URN)10.1016/S0168-9452(03)00072-4 (DOI)
Available from: 2015-06-24 Created: 2015-06-24 Last updated: 2017-12-04
Mutisya, J., Sathish, P., Sun, C., Andersson, L., Ahlandsberg, S., Baguma, Y., . . . Jansson, C. (2003). Starch branching enzymes in sorghum (Sorghum bicolor) and barley (Hordeum vulgare): Comparative analyses of enzyme structure and gene expression. Journal of plant physiology (Print), 160(8), 921-930
Open this publication in new window or tab >>Starch branching enzymes in sorghum (Sorghum bicolor) and barley (Hordeum vulgare): Comparative analyses of enzyme structure and gene expression
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2003 (English)In: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 160, no 8, p. 921-930Article in journal (Refereed) Published
Abstract [en]

Summary A genomic clone for starch branching enzyme (SBE) IIb was isolated from a sorghum bacterial artificial chromosome (BAC) library. The promoter and 5′ flanking sequence, the first four exons and introns as well as the last exon and the 3′ untranslated region were sequenced. The tentative transcription start site of sorghum sbeIIb was mapped based on alignment with the maize sbeIIb gene. The exon-intron structure of the 5′ portion of sorghum sbeIIb was similar to that of maize sbeIIb but differed from that of barley sbeIIb. Specifically, the intronic Bbl element involved in the endosperm specific expression of barley sbeIIb was lacking in the sorghum gene. A cDNA clone for sorghum sbeIIb was reverse PCR amplified and found to encode an 803 amino acids long protein. The amino acid sequence of sorghum SBEIIb was compared to that of sorghum SBEI and corresponding enzymes in barley. The overall identity in amino acid sequence was 54 percnt; in the central portion of the enzymes. A major difference between the SBEII and SBEI isoforms was a 67 amino acids-long C-terminal extension in the SBEIs. The spatial and temporal expression patterns of sorghum sbeIIb was determined and compared with those of the sorghum gene for SBEI and the barley genes for SBEIIB and SBEI. All four genes exhibited a seed specific expression. However, while barley sbeIIb and sbeI transcripts were detected exclusively in the endosperm, the sorghum genes were expressed also in the embryo. The activity of sorghum sbeIIb and sbeI exhibited a late onset, with a peak of transcription at around 22 days after pollination. This is similar to the pattern of barley sbeI but different from that of barley sbeIIb, which showed a peak of transcription at 12 days after pollination.

Keywords
sorghum, starch branching enzyme
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-256618 (URN)10.1078/0176-1617-00960 (DOI)
Available from: 2015-06-24 Created: 2015-06-24 Last updated: 2017-12-04
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Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-7648-9103

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