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Eriksson, Camilla
Publications (9 of 9) Show all publications
Jacobsson, E., Andersson, H. S., Strand, M., Peigneur, S., Eriksson, C., Lodén, H., . . . Göransson, U. (2018). Peptide ion channel toxins from the bootlace worm, the longest animal on Earth. Scientific Reports, 8, Article ID 4596.
Open this publication in new window or tab >>Peptide ion channel toxins from the bootlace worm, the longest animal on Earth
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2018 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, article id 4596Article in journal (Refereed) Published
Abstract [en]

Polypeptides from animal venoms have found important uses as drugs, pharmacological tools, and within biotechnological and agricultural applications. We here report a novel family of cystine knot peptides from nemertean worms, with potent activity on voltage-gated sodium channels. These toxins, named the alpha-nemertides, were discovered in the epidermal mucus of Lineus longissimus, the 'bootlace worm' known as the longest animal on earth. The most abundant peptide, the 31-residue long alpha-1, was isolated, synthesized, and its 3D NMR structure determined. Transcriptome analysis including 17 species revealed eight alpha-nemertides, mainly distributed in the genus Lineus. alpha-1 caused paralysis and death in green crabs (Carcinus maenas) at 1 mu g/kg (similar to 300 pmol/kg). It showed profound effect on invertebrate voltage-gated sodium channels (e.g. Blattella germanica Na(v)1) at low nanomolar concentrations. Strong selectivity for insect over human sodium channels indicates that a-nemertides can be promising candidates for development of bioinsecticidal agents.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP, 2018
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-351585 (URN)10.1038/s41598-018-22305-w (DOI)000428029600001 ()29567943 (PubMedID)
Funder
Swedish Research Council, 2014-3327]
Available from: 2018-05-29 Created: 2018-05-29 Last updated: 2019-08-15Bibliographically approved
Wang, Y., Lloyd, K. A., Gunasekera, S., Eriksson, C., Ramsköld, D., Lundberg, K., . . . Grönwall, C. (2018). Repertoire Studies in Rheumatoid Arthritis Reveal B-Cell Distortions and Baseline Shifts in Unmutated IgG. Arthritis & Rheumatology, 70
Open this publication in new window or tab >>Repertoire Studies in Rheumatoid Arthritis Reveal B-Cell Distortions and Baseline Shifts in Unmutated IgG
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2018 (English)In: Arthritis & Rheumatology, ISSN 2326-5191, E-ISSN 2326-5205, Vol. 70Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
John Wiley & Sons, 2018
National Category
Rheumatology and Autoimmunity
Identifiers
urn:nbn:se:uu:diva-369631 (URN)000447268900009 ()
Available from: 2018-12-17 Created: 2018-12-17 Last updated: 2018-12-17Bibliographically approved
Gunasekera, S., Fernandes-Cerqueira, C., Eriksson, C., Jakobsson, P.-J. & Göransson, U. (2016). Anti-Citrullinated Peptide Antibody Inhibitors Based On Sunflower Trypsin Inhibitor-1 Scaffold For Potential Anti-Rheumatoid Arthritis Activity. Journal of Peptide Science, 22(S2), S170-S170
Open this publication in new window or tab >>Anti-Citrullinated Peptide Antibody Inhibitors Based On Sunflower Trypsin Inhibitor-1 Scaffold For Potential Anti-Rheumatoid Arthritis Activity
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2016 (English)In: Journal of Peptide Science, ISSN 1075-2617, E-ISSN 1099-1387, Vol. 22, no S2, p. S170-S170Article in journal, Meeting abstract (Other academic) Published
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-331382 (URN)10.1002/psc.2950 (DOI)000398216100317 ()
Available from: 2017-10-17 Created: 2017-10-17 Last updated: 2017-10-17Bibliographically approved
Gunasekera, S., Fernandes-Cerqueira, C., Eriksson, C., Jakobsson, P. J. & Göransson, U. (2016). Circular disulfide-rich peptide scaffolds as anti-citrullinated peptide antibody inhibitors. Paper presented at 9th Joint Meeting of AFERP, ASP, GA, JSP, PSE and SIF, JUL 24-27, 2016, Copenhagen, DENMARK. Planta Medica, 82
Open this publication in new window or tab >>Circular disulfide-rich peptide scaffolds as anti-citrullinated peptide antibody inhibitors
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2016 (English)In: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 82Article in journal, Meeting abstract (Other academic) Published
Keywords
anti-citrullinated protein/peptide antibodies (ACPA), cyclic peptide, fibrinogen, NMR, target, sunflower trypsin inhibitor 1 (SFTI-1)
National Category
Pharmacology and Toxicology
Identifiers
urn:nbn:se:uu:diva-346853 (URN)10.1055/s-0036-1596779 (DOI)000411789300603 ()
Conference
9th Joint Meeting of AFERP, ASP, GA, JSP, PSE and SIF, JUL 24-27, 2016, Copenhagen, DENMARK
Available from: 2018-03-27 Created: 2018-03-27 Last updated: 2018-03-27Bibliographically approved
Melander, E., Eriksson, C., Jansson, B., Göransson, U. & Hammarlund-Udenaes, M. (2016). Improved method for quantitative analysis of the cyclotide kalata B1 in plasma and brain homogenate. Paper presented at 3rd International Conference on Circular Proteins (ICCP), NOV 01-04, 2015, AUSTRALIA. Biopolymers, 106(6), 910-916
Open this publication in new window or tab >>Improved method for quantitative analysis of the cyclotide kalata B1 in plasma and brain homogenate
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2016 (English)In: Biopolymers, ISSN 0006-3525, E-ISSN 1097-0282, Vol. 106, no 6, p. 910-916Article in journal, Meeting abstract (Refereed) Published
Abstract [en]

This study provides a new method for quantifying the cyclotide kalata B1 in both plasma and brain homogenate. Cyclotides are ultra-stable peptides with three disulfide bonds that are interesting from a drug development perspective as they can be used as scaffolds. In this study we describe a new validated LC-MS/MS method with high sensitivity and specificity for kalata B1. The limit of quantification was 2 ng/mL in plasma and 5 ng/gmL in brain homogenate. The method was linear in the range 2-10,000 ng/mL for plasma and 5-2000 ng/g for brain. Liquid Chromatographic separation was performed on a HyPurity C18 column, 50 3 4.6 mm, 3 mm particle size. The method had inter-and intra-day precision and accuracy levels <15% and 12% respectively. Applying the method to in vivo plasma samples and brain homogenate samples from equilibrium dialysis yielded satisfying results and was able to describe the plasma pharmacokinetics and brain tissue binding of kalata B1. The described method is quick, reproducible and well suited to quantifying kalata B1 in biological matrices.

Keywords
brain, cyclotides, kalata B1, liquid chromatography, mass spectrometry, pharmacokinetics
National Category
Medicinal Chemistry
Identifiers
urn:nbn:se:uu:diva-317707 (URN)10.1002/bip.22984 (DOI)000393465500016 ()27603276 (PubMedID)
Conference
3rd International Conference on Circular Proteins (ICCP), NOV 01-04, 2015, AUSTRALIA
Funder
Swedish Research Council, 2012-5063 2011-4339
Available from: 2017-03-17 Created: 2017-03-17 Last updated: 2018-01-13Bibliographically approved
Göransson, U., Gunasekera, S., Malik, S., Park, S., Slazak, B., Jacobsson, E., . . . Strömstedt, A. A. (2016). Peptide biodiscovery from plants and animals: structure to function. Paper presented at 9th Joint Meeting of AFERP, ASP, GA, JSP, PSE and SIF, JUL 24-27, 2016, Copenhagen, DENMARK. Planta Medica, 82
Open this publication in new window or tab >>Peptide biodiscovery from plants and animals: structure to function
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2016 (English)In: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 82Article in journal, Meeting abstract (Other academic) Published
Keywords
Peptide biodiscovery, cyclotides, sponge peptides, cacti
National Category
Basic Medicine
Identifiers
urn:nbn:se:uu:diva-346852 (URN)10.1055/s-0036-1596156 (DOI)000411789300953 ()
Conference
9th Joint Meeting of AFERP, ASP, GA, JSP, PSE and SIF, JUL 24-27, 2016, Copenhagen, DENMARK
Available from: 2018-03-27 Created: 2018-03-27 Last updated: 2018-03-27Bibliographically approved
Jacobsson, E., Andersson, H. S., Strand, M., Lebbe, E., Eriksson, C., Peigneur, S., . . . Göransson, U. (2016). Peptide toxins from the longest animal on earth. Paper presented at 9th Joint Meeting of AFERP, ASP, GA, JSP, PSE and SIF, JUL 24-27, 2016, Copenhagen, DENMARK. Planta Medica, 82
Open this publication in new window or tab >>Peptide toxins from the longest animal on earth
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2016 (English)In: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 82Article in journal, Meeting abstract (Other academic) Published
Keywords
peptide toxin, nemertea, neurotoxin
National Category
Pharmacology and Toxicology
Identifiers
urn:nbn:se:uu:diva-346855 (URN)10.1055/s-0036-1596160 (DOI)000411789300986 ()
Conference
9th Joint Meeting of AFERP, ASP, GA, JSP, PSE and SIF, JUL 24-27, 2016, Copenhagen, DENMARK
Available from: 2018-03-26 Created: 2018-03-26 Last updated: 2018-03-26Bibliographically approved
Eriksson, C., Gunasekera, S., Cerqueira, C., Jacobsson, P. J. & Göransson, U. (2016). Synthesis of cyclic citrullinated peptides targeting rheumatoid arthritis autoantibodies based on sunflower trypsin inhibitors. Paper presented at 9th Joint Meeting of AFERP, ASP, GA, JSP, PSE and SIF, JUL 24-27, 2016, Copenhagen, DENMARK. Planta Medica, 82
Open this publication in new window or tab >>Synthesis of cyclic citrullinated peptides targeting rheumatoid arthritis autoantibodies based on sunflower trypsin inhibitors
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2016 (English)In: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 82Article in journal, Meeting abstract (Other academic) Published
Keywords
ACPA, rheumatoid arthritis, citrullination, SFTI-1
National Category
Pharmacology and Toxicology
Identifiers
urn:nbn:se:uu:diva-346851 (URN)10.1055/s-0036-1596748 (DOI)000411789300572 ()
Conference
9th Joint Meeting of AFERP, ASP, GA, JSP, PSE and SIF, JUL 24-27, 2016, Copenhagen, DENMARK
Available from: 2018-03-27 Created: 2018-03-27 Last updated: 2018-03-27Bibliographically approved
Andersson, H. S., Jacobsson, E., Eriksson, C., Hedstrom, M., Seth, H., Sundberg, P., . . . Göransson, U. (2016). The toxicity of ribbon worms: Alpha-nemertides or tetrodotoxin, or both?. Paper presented at 9th Joint Meeting of AFERP, ASP, GA, JSP, PSE and SIF, JUL 24-27, 2016, Copenhagen, DENMARK. Planta Medica, 82
Open this publication in new window or tab >>The toxicity of ribbon worms: Alpha-nemertides or tetrodotoxin, or both?
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2016 (English)In: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 82Article in journal, Meeting abstract (Other academic) Published
National Category
Pharmacology and Toxicology
Identifiers
urn:nbn:se:uu:diva-346848 (URN)10.1055/s-0036-1596617 (DOI)000411789300441 ()
Conference
9th Joint Meeting of AFERP, ASP, GA, JSP, PSE and SIF, JUL 24-27, 2016, Copenhagen, DENMARK
Available from: 2018-03-27 Created: 2018-03-27 Last updated: 2018-03-27Bibliographically approved
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