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BETA
Andersson, Marit
Alternative names
Publications (10 of 16) Show all publications
Korvela, M., Andersson, M. & Pettersson, J. (2018). Internal standards in inductively coupled plasma mass spectrometry using kinetic energy discrimination and dynamic reaction cells. Journal of Analytical Atomic Spectrometry, 33(10), 1770-1776
Open this publication in new window or tab >>Internal standards in inductively coupled plasma mass spectrometry using kinetic energy discrimination and dynamic reaction cells
2018 (English)In: Journal of Analytical Atomic Spectrometry, ISSN 0267-9477, E-ISSN 1364-5544, Vol. 33, no 10, p. 1770-1776Article in journal (Refereed) Published
Abstract [en]

ICP-MS is a sensitive element analysis technique used for analyzing several different sample types. This can result in difficult matrixes which can affect both physical parameters and create overlaps of analyte elements. Some of the possible overlaps can be reduced by the use of reaction and/or collision cells, while the use of internal standards can help with reducing the physical interferences caused by a matrix. While both internal standardization and the use of cells have been studied separately, their effects on each other have not been investigated earlier. In this study ICP-MS was used to analyze Mg-24, Al-27, Ti-47, Ti-49, V-51, Cr-52, Cr-53, Mn-55, Fe-57, Co-59, Ni-60, Ni-61, Ni-62, Cu-63, Cu-65, Zn-66, Zn-67, As-75, Se-78, Se-82, Cd-111, and Pb-208 with Be-9, Y-89, Ga-69, Rh-103, In-115, Ir-193, and Tl-205 as internal standards with high concentrations of either HNO3, PBS-buffer, or Triton X-100 as the matrix, in reaction-, collision- and standard-cell modes. This was done to investigate which internal standards would compensate matrix effects in different cell modes. All internal standards, except Be, compensated fairly well (relative sensitivity RSD < 10%) even for severe matrix effects for most elements regardless of similarity in mass in the different cell modes. For Zn, As and Se no proper internal standard could be found, of the ones investigated.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY, 2018
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-369509 (URN)10.1039/c8ja00171e (DOI)000448340200019 ()
Available from: 2018-12-14 Created: 2018-12-14 Last updated: 2019-10-10Bibliographically approved
Taube, A. B., Hardenborg, E., Wetterhall, M., Artemenko, K., Hanrieder, J., Andersson, M., . . . Bergquist, J. (2012). Proteins in aqueous humor from cataract patients with and without pseudoexfoliation syndrome. European journal of mass spectrometry, 18(6), 531-541
Open this publication in new window or tab >>Proteins in aqueous humor from cataract patients with and without pseudoexfoliation syndrome
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2012 (English)In: European journal of mass spectrometry, ISSN 1469-0667, E-ISSN 1751-6838, Vol. 18, no 6, p. 531-541Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to investigate the protein content in aqueous humor in eyes with and without pseudoexfoliations (PEX) and to evaluate the quantitative proteomics method, isobaric tagging for relative and absolute protein quantification (iTRAQ), in combination with two separation methods followed by matrix-assisted Laser desorption/ionization (MALDI) mass spectrometry and tandem mass spectrometry (MS/MS). During cataract surgery, samples of aqueous humor were collected from 20 eyes with PEX and from 18 control eyes. The relative concentrations of proteins in the pooled samples of ten PEX eyes and eight controls were evaluated after trypsin digestion and labeling of the peptides with (iTRAQ) reagent. Two separation methods, Liquid chromatography (LC) and capillary electrophoresis (CE) were used, followed by MALDI mass spectrometry and MS/MS. Furthermore, 1D gel electrophoresis was performed on the remaining ten pooled PEX samples and ten control samples. The gel material was separated by nano-liquid chromatography (nano-LC) followed by Linear-ion-trap quadrupole Fourier transformation ion cyclotron resonance (FT-ICR). Fifty four proteins were identified in the LC runs and 24 with CE. The relative concentrations of beta-crystallines B2 and S were raised and those of angiotensinogen and osteopontin lowered in the PEX sample compared to the control. The trends regarding beta-crystallines B2, angiotensinogen and osteopontin were confirmed by the 1D gel electrophoresis.

Keywords
pseudoexfoliations (PEX), isobaric tagging, protein quantification, proteomics, aqueous humour, osteopontin, angiotensinogen
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-197670 (URN)10.1255/ejms.1208 (DOI)000315745600007 ()
Available from: 2013-04-02 Created: 2013-04-02 Last updated: 2017-12-06Bibliographically approved
Kollander, B., Andersson, M. & Pettersson, J. (2011). Application of a fast multi element screening method using ICP-AES on liver samples and mice organs. Journal of Trace Elements in Medicine and Biology
Open this publication in new window or tab >>Application of a fast multi element screening method using ICP-AES on liver samples and mice organs
2011 (English)In: Journal of Trace Elements in Medicine and Biology, ISSN 0946-672X, E-ISSN 1878-3252Article in journal (Other academic) Submitted
Abstract [en]

A fast mix and measure method for direct multi element analysis of non digested biological samples was used for the simultaneous determination of low, medium, and high concentrations elements in liver samples from domestic and wild animals. The method was also used for screening several elements in different organs from five mice. Brain (cerebrum, left and right cerebellum), heart, kidney, liver, lung, spleen, and blood samples were analysed. The total time for sample preparation and measurement is four minutes only for each sample (dissection not included). Quantitative results within ± 10 % are presented in the liver samples for Al, Cd, Pb, Mn, Sr, P, K, and semi quantitative within ± 20 % for Ca, Co, Cu, Fe, Mg, and Zn. The overall reproducibility was around 5 % for most elements, slightly higher for elements close to the detection limit, which meets well with the 2-3 % obtained for the acid digestion method used for comparison. A bovine liver sample was spiked with low amounts of Al, Cd, Co, and Pb to outline the possibilities of the slurry method to quantify concentrations close to the estimated limit of quantification. The recoveries were 103, 104, 97 and 103 % respectively.

Place, publisher, year, edition, pages
Elsevier, 2011
Keywords
multi element, screening, medical samples, biological samples, ICP-AES, inductively coupled plasma atomic emission spectrometry, liver, mice
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-150837 (URN)
Note

pubkoll2017

Available from: 2011-04-06 Created: 2011-04-06 Last updated: 2017-05-15Bibliographically approved
Kollander, B., Andersson, M. & Pettersson, J. (2010). Fast multi-element screening of non-digested biological materials by slurry introduction to ICP-AES.. Talanta: The International Journal of Pure and Applied Analytical Chemistry, 80(5), 2068-2075
Open this publication in new window or tab >>Fast multi-element screening of non-digested biological materials by slurry introduction to ICP-AES.
2010 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 80, no 5, p. 2068-2075Article in journal (Refereed) Published
Abstract [en]

A fast method for direct multi-element analysis of non-digested biological samples is presented. The only sample preparation needed is 1 min homogenization with a Polytron mixer in a small volume of neutral phosphate buffer saline solution (PBS). The total time for analysis (sample preparation and measurement) is 4 min only. This "mix and measure" method can handle large sample loads of biological samples and thus minimize dilution of trace elements. For example 100% whole blood was introduced without any clogging of the introduction system or extinguishing of the plasma. In 70% (v/v) whole blood reference material 14 of 16 analytes were quantified within ±10% (Al, B, Ba, Ca, Cu, Fe, Mg, Mn, P, Pb, S, Sr, Ti and Zn) and two semi-quantified within ±20% (Cd and K). Fresh bovine liver was also analyzed with the same method and 7 of 9 analytes were quantified in 5% (w/v) liver slurry. Three different nebulizers were tested, Glass Expansion Concentric (GEC) of Meinhard type, Cross Flow and Burgener T2100 and they performed roughly equally well in giving quantitative results for the slurries but the sensitivity was better with the GEC. The stability of the plasma was studied by evaluating the ratio of Mg 280.270 nm and Mg 285.213 nm lines. When increasing the sample load from 20 to 100% (v/v) of whole blood and from 0.5 to 10% (w/v) of bovine liver the Mg ratio was constant within a few percent for all of the nebulizer tested. The ratio of the sensitivity between GEC and Burgener T2100 was studied and the ratio increased with the energy sum for atomic and ionic lines separately.

Keywords
External calibration, ICP-AES, internal standards, liver, slurry, whole blood
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-122404 (URN)10.1016/j.talanta.2009.11.007 (DOI)000275612500073 ()20152454 (PubMedID)
Available from: 2010-04-12 Created: 2010-04-12 Last updated: 2017-12-12Bibliographically approved
Hardenborg, E., Taube, A. B., Hanrieder, J., Andersson, M., Alm, A. & Bergquist, J. (2009). Protein content in aqueous humor from patients with pseudoexfoliation (PEX) investigated by capillary-LC MALDI-TOF/TOF MS. PROTEOMICS - Clinical Applications, 3(3), 299-306
Open this publication in new window or tab >>Protein content in aqueous humor from patients with pseudoexfoliation (PEX) investigated by capillary-LC MALDI-TOF/TOF MS
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2009 (English)In: PROTEOMICS - Clinical Applications, ISSN 1862-8346, E-ISSN 1862-8354, Vol. 3, no 3, p. 299-306Article in journal (Refereed) Published
Abstract [en]

Analysis of proteins in human body fluids is challenging since the composition of the sample often is rather complex. Here we present a method for analysis of proteins in aqueous humor from two groups of cataract patients, with and without pseudoexfoliation (PEX). Aqueous humor is an extracellular fluid contained in the anterior chamber of the eye between the cornea and iris. The limited volume of sample requires sophisticated analysis techniques. Our method is based on a total tryptic digestion of the sample followed by capillary LC-MALDI MS and MS/MS analysis of the peptides. The method is rapid, efficient and suitable as a complement or alternative to more commonly used methods based on gel electrophoretic experiments. With this method we found and unambiguously identified 30 nonredundant proteins. Proteins found include general transport proteins such as albumin and apolipoprotein A1 but also specific proteins involved in immune response, such as   complement factors. Cystatin C, clusterin, and crystallins were also found. Although the number of proteins was roughly the same in both groups there was a significant difference in their identities. These findings may give some new insights into the pathophysiology of the PEX syndrome.

Keywords
Aqueous humor, Capillary liquid chromatography, Human body fluids, MALDI-TOF MS/MS, Pseudoexfoliation syndrome
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-97814 (URN)10.1002/prca.200780077 (DOI)000264794700002 ()
Available from: 2008-11-20 Created: 2008-11-20 Last updated: 2017-12-14Bibliographically approved
Forsgard, N., Sjöberg, P., Bylund, D., Andersson, M. & Pettersson, J. (2007). Screening and identification of aluminium-containing biomolecules by column-switched LC-ICP-MS and LC-ESI-MS/MS. Journal of Analytical Atomic Spectrometry, 22(11), 1397-1402
Open this publication in new window or tab >>Screening and identification of aluminium-containing biomolecules by column-switched LC-ICP-MS and LC-ESI-MS/MS
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2007 (English)In: Journal of Analytical Atomic Spectrometry, ISSN 0267-9477, E-ISSN 1364-5544, Vol. 22, no 11, p. 1397-1402Article in journal (Refereed) Published
Abstract [en]

Column-switching liquid chromatography followed by low resolution ICP-MS was evaluated as a tool for speciation analysis of aluminium-containing biomolecules. The strategy was applied on siderophores, small organic molecules (Mr < 1500) which normally act as strong iron chelators. The drawbacks normally encounterd with aluminium detection using low resolution ICP-MS are the formation of polyatomic ions causing isobaric overlaps and space-charge effects. When adding a carbon rich solvent, such as methanol or acetonitrile, the 13C14N+, 12C15N+ and 12C14N1H+ with the same mass as 27Al+ will form in the plasma. The nitrogen is either entrained from the surrounding atmosphere or added with the constituents in the mobile phase. These disadvantages were successfully counteracted by the use of nitrogen free organic modifier in the mobile phase and the use of cool plasma conditions. Detection limits for standard solutions of aluminium-chelated ferrichrome in sub-nanomolar range were obtained by monitoring the aluminium-27 isotope. The combined use of LC-ICP-MS and LC-ESI-MS/MS was also evaluated as a tool to identify unknown metal complexes, here siderophores, in field soil solution samples. Two aluminium-chelated siderophores, Al-desferrichrom and Al-desferricrocin, were identified and quantified. Both aluminium-siderophore complexes were present in the low nanomolar range (1.1 and 0.7 nM, respectively).

National Category
Chemical Sciences
Identifiers
urn:nbn:se:uu:diva-96098 (URN)10.1039/b707948f (DOI)000250399600015 ()
Available from: 2007-09-04 Created: 2007-09-04 Last updated: 2017-12-14Bibliographically approved
Bergström, S. K., Dahlin, A., Ramström, M., Andersson, M., Markides, K. & Bergquist, J. (2006). A simplified multidimensional approach for analysis of complex biological samples: on-line LC-CE-MS. The Analyst, 131(7), 791-798
Open this publication in new window or tab >>A simplified multidimensional approach for analysis of complex biological samples: on-line LC-CE-MS
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2006 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 131, no 7, p. 791-798Article in journal (Refereed) Published
Abstract [en]

Information on protein expression, disease biomarkers or surrogate markers and genetic disorders can nowadays be achieved from analysis of complex biological samples by liquid separation coupled to mass spectrometric (MS) detection. This paper describes fast multidimensional separation by on-line liquid chromatography (LC) and capillary electrophoresis (CE), followed by electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) MS detection. This detector provides ultrahigh resolution of the detected ions, mass accuracy at the ppm-level and high sensitivity. Most of the challenge of this system lies in the development of a new interface for the on-line coupling of LC to CE. The interface developed in poly(dimethylsiloxane) provides a RSD for injection repeatability of <3.5% and surface control for unspecific binding by deactivation with a cationic polymer, PolyE-323. We have evaluated the interface, as well as the overall system, with respect to robustness and deconvolution ability. Sequence coverage for bovine serum albumin (BSA) of 93% showed a high recovery of sample in the different transfer steps through the system. The detection limit for identification is 277 ng mL−1 (or 280 nM) on average for peptides. In the future, we expect LC-CE-MS to be a novel strategy for elucidating the chemistry of biological matrices.

National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-80932 (URN)10.1039/b601660j (DOI)
Available from: 2006-06-29 Created: 2006-06-29 Last updated: 2017-12-14Bibliographically approved
Forsgard, N., Nilsson, E., Andersson, M. & Pettersson, J. (2006). Investigation of matrix effects in boron determination using organic solvents as modifiers for liquid chromatography coupled to ICP-MS. Journal of Analytical Atomic Spectroscopy (21), 305-310
Open this publication in new window or tab >>Investigation of matrix effects in boron determination using organic solvents as modifiers for liquid chromatography coupled to ICP-MS
2006 (Swedish)In: Journal of Analytical Atomic Spectroscopy, no 21, p. 305-310Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-80762 (URN)doi:10.1039/b513753e (DOI)
Available from: 2006-05-23 Created: 2006-05-23 Last updated: 2011-01-11
Bergström, S. K., Goiny, M., Danielsson, R., Ungerstedt, U., Andersson, M. & Markides, K. E. (2006). Screening of microdialysates taken before and after induced liver damage; on-line solid phase extraction-electrospray ionization-mass spectrometry. Journal of Chromatography A, 1120(1-2), 21-26
Open this publication in new window or tab >>Screening of microdialysates taken before and after induced liver damage; on-line solid phase extraction-electrospray ionization-mass spectrometry
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2006 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1120, no 1-2, p. 21-26Article in journal (Refereed) Published
Abstract [en]

A novel method is described to follow known and unknown compounds in biological processes using microdialysis sampling and mass spectrometric detection. By implementation of internal standard, desalting/enrichment for the sample work-up, and multivariate data analysis, this methodology is a basis for future applications in early diagnosis of diseases and organ damage, as a complement to the routinely used clinical methods for biological samples. The present study includes screening without specific target analytes, of samples collected by microdialysis from liver of anaesthetized rats before and after local damage to this organ. Sample series were classified by principal component analysis, and the stimulation was identified in the chemical patterns produced by the presented analytical tool.

Keywords
In vivo microdialysis, Screening, Biomarkers, On-line desalting, Mass Spectrometry, Chemometrics
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-81344 (URN)10.1016/j.chroma.2006.01.110 (DOI)
Available from: 2006-08-17 Created: 2006-08-17 Last updated: 2017-12-14Bibliographically approved
Ribbing, C., Andersson, M., Hjort, K. & Lundqvist, H. (2003). A miniature X-ray fluorescence set-up. Review of Scientific Instruments, 74, 3423-3428
Open this publication in new window or tab >>A miniature X-ray fluorescence set-up
2003 (English)In: Review of Scientific Instruments, Vol. 74, p. 3423-3428Article in journal (Refereed) Published
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-47048 (URN)
Available from: 2007-04-23 Created: 2007-04-23 Last updated: 2011-01-13
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