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Balgoma, David
Publications (2 of 2) Show all publications
Balgoma, D., Pettersson, C. & Hedeland, M. (2019). Common Fatty Markers in Diseases with Dysregulated Lipogenesis. Trends in endocrinology and metabolism, 30(5), 283-285
Open this publication in new window or tab >>Common Fatty Markers in Diseases with Dysregulated Lipogenesis
2019 (English)In: Trends in endocrinology and metabolism, ISSN 1043-2760, E-ISSN 1879-3061, Vol. 30, no 5, p. 283-285Article in journal, Editorial material (Refereed) Published
Abstract [en]

Recent studies have reported the upregulation of a subgroup of triacylglycerides as markers of different diseases with dysregulated lipogenesis, which means that these markers are not selective. This observation has a deep impact on their use as diagnostic tools in clinical practice (e.g., markers of risk of type 2 diabetes).

Place, publisher, year, edition, pages
ELSEVIER SCIENCE LONDON, 2019
National Category
Endocrinology and Diabetes Medicinal Chemistry
Identifiers
urn:nbn:se:uu:diva-383044 (URN)10.1016/j.tem.2019.02.008 (DOI)000465043400001 ()30926249 (PubMedID)
Available from: 2019-05-13 Created: 2019-05-13 Last updated: 2020-01-31Bibliographically approved
Balgoma, D., Guitton, Y., Evans, J. J., Le Bizec, B., Dervilly-Pinel, G. & Meynier, A. (2019). Modeling the fragmentation patterns of triacylglycerides in mass spectrometry allows the quantification of the regioisomers with a minimal number of standards. Analytica Chimica Acta, 1057, 60-69
Open this publication in new window or tab >>Modeling the fragmentation patterns of triacylglycerides in mass spectrometry allows the quantification of the regioisomers with a minimal number of standards
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2019 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 1057, p. 60-69Article in journal (Refereed) Published
Abstract [en]

Mass spectrometry allows the relative quantification of the regioisomers of triacylglycerides by the calibration of their fragmentation patterns. However, due to the plethora of regioisomers of triacylglycerides, calibration with every standard is not feasible. An analytical challenge in the field is the prediction of the fragmentation patterns of triacylglycerides to quantify their regioisomers. Thus, we aimed to model these fragmentation patterns to quantify the regioisomeric composition, even for those without commercially available standards. In a first step, we modeled the fragmentation patterns of the regioisomers of triacylglycerides obtained from different published datasets. We found the same qualitative trends of fragmentation beyond differences in the type of adduct in these datasets (both [M+NH4]+ and [M+H]+), and the type of instrument (orbitrap, Q-ToF, ion-trap, single quadrupole, and triple quadrupole). However, the quantitative trends of fragmentation were adduct and instrument specific. From these observations, we modeled quantitatively the common trends of fragmentation of triacylglycerides in every dataset. In a second step, we applied this methodology on a Synapt G2S Q-ToF to quantify the regioisomers of triacylglycerides in sunflower and olive oils. The results of our quantification were in good agreement with previous published quantifications of triacylglycerides, even for regioisomers that were not present in the training dataset. The species with more than two highly unsaturated fatty acids (arachidonic, eicosapentaenoic, and docosahexaenoic acids) showed a complex behavior and lower predictability of their fragmentation patterns. However, this framework presents the capacity to model this behavior when more data are available. It would be also applicable to standardize the quantification of the regioisomers of triacylglycerides in an inter-laboratory ring study.

Keywords
Triacylglycerols, Positional isomers, Regioisomers, Lipidomics, Modeling
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-379322 (URN)10.1016/j.aca.2019.01.017 (DOI)000459978400007 ()30832919 (PubMedID)
Available from: 2019-03-28 Created: 2019-03-28 Last updated: 2019-03-28Bibliographically approved
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