uu.seUppsala universitets publikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Activation of the human c-kit product by ligand-induced dimerization mediates circular actin reorganization and chemotaxis
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Ludwiginstitutet för cancerforskning.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Ludwiginstitutet för cancerforskning.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Klinisk kemi.
AMGEN Inc., AMGEN Center, Thousand Oaks, CA 91320, USA.
Vise andre og tillknytning
1991 (engelsk)Inngår i: EMBO Journal, ISSN 0261-4189, E-ISSN 1460-2075, Vol. 10, nr 13, s. 4121-4128Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The proto-oncogene c-kit is allelic with the murine white spotting (W) locus and encodes a transmembrane protein tyrosine kinase that is structurally related to the receptors for platelet-derived growth factor (PDGF) and colony-stimulating factor-1 (CSF-1). Recently the ligand for the c-kit product, stem cell factor (SCF), was identified in both transmembrane and soluble forms. In order to examine the mechanism for receptor activation by SCF and biological properties of the activated c-kit product, we transfected the wild-type human c-kit cDNA into porcine aortic endothelial cells. We found that the receptor was down-regulated and transmitted a mitogenic signal in response to stimulation with soluble SCF. We also demonstrate that SCF induces dimerization of the c-kit product in intact cells, and that dimerization of the receptor is correlated with activation of its kinase. Activation of the c-kit product by SCF was found to induce circular actin reorganization indistinguishable from that mediated by the PDGF beta-receptor in response to PDGF-BB. Furthermore, soluble SCF was a potent chemotactic agent for cells expressing the c-kit product, a property which might be of importance during embryonic development.

sted, utgiver, år, opplag, sider
1991. Vol. 10, nr 13, s. 4121-4128
Emneord [en]
Actins/*metabolism, Animals, Blotting; Western, Cells; Cultured, Chemotaxis, Cross-Linking Reagents, Cytoskeleton/metabolism, Electrophoresis; Polyacrylamide Gel, Endothelium; Vascular/cytology/metabolism, Hematopoietic Cell Growth Factors/*pharmacology, Humans, Ligands, Phosphorylation, Precipitin Tests, Protein-Tyrosine Kinase/*metabolism, Proto-Oncogene Protein c-kit, Proto-Oncogene Proteins/biosynthesis/*metabolism, Recombinant Proteins/pharmacology, Research Support; Non-U.S. Gov't, Stem Cell Factor, Swine, Thymidine/metabolism
HSV kategori
Identifikatorer
URN: urn:nbn:se:uu:diva-73230PubMedID: 1721869OAI: oai:DiVA.org:uu-73230DiVA, id: diva2:101141
Tilgjengelig fra: 2005-06-01 Laget: 2005-06-01 Sist oppdatert: 2017-12-14bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

PubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=1721869&dopt=Citation

Personposter BETA

Claesson-Welsh, LenaSiegbahn, AgnetaWestermark, BengtHeldin, Carl-Henrik

Søk i DiVA

Av forfatter/redaktør
Claesson-Welsh, LenaSiegbahn, AgnetaWestermark, BengtHeldin, Carl-Henrik
Av organisasjonen
I samme tidsskrift
EMBO Journal

Søk utenfor DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetric

pubmed
urn-nbn
Totalt: 1132 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf