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De novo peptide sequencing and identification with precision mass spectrometry
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för farmaceutisk biovetenskap, MMS, medicinsk masspektrometri.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för farmaceutisk biovetenskap, MMS, medicinsk masspektrometri.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för farmaceutisk biovetenskap, MMS, medicinsk masspektrometri.
Vise andre og tillknytning
2007 (engelsk)Inngår i: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 6, nr 1, s. 114-123Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The recent proliferation of novel mass spectrometers such as Fourier transform, QTOF, and OrbiTrap marks a transition into the era of precision mass spectrometry, providing a 2 orders of magnitude boost to the mass resolution, as compared to low-precision ion-trap detectors. We investigate peptide de novo sequencing by precision mass spectrometry and explore some of the differences when compared to analysis of low-precision data. We demonstrate how the dramatically improved performance of de novo sequencing with precision mass spectrometry paves the way for novel approaches to peptide identification that are based on direct sequence lookups, rather than comparisons of spectra to a database. With the direct sequence lookup, it is not only possible to search a database very efficiently, but also to use the database in novel ways, such as searching for products of alternative splicing or products of fusion proteins in cancer. Our de novo sequencing software is available for download at http://peptide.ucsd.edu/.

sted, utgiver, år, opplag, sider
2007. Vol. 6, nr 1, s. 114-123
Emneord [en]
precision MS, MS/MS, de novo, database search, pattern matching, filtration, FTMS, FT-ICR, LTQ-FT
HSV kategori
Identifikatorer
URN: urn:nbn:se:uu:diva-146543DOI: 10.1021/pr060271uISI: 000243262600011PubMedID: 17203955OAI: oai:DiVA.org:uu-146543DiVA, id: diva2:398412
Tilgjengelig fra: 2011-02-17 Laget: 2011-02-17 Sist oppdatert: 2017-12-11bibliografisk kontrollert

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