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High Cysteine Proteins are up-regulated during Giardia-host cell interaction.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för cell- och molekylärbiologi, Mikrobiologi.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för cell- och molekylärbiologi, Mikrobiologi.
Uppsala universitet, Science for Life Laboratory, SciLifeLab. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för cell- och molekylärbiologi, Molekylär evolution.
Eukaryotic Single Cell Genomics Platform, Karolinska Institute, SciLifeLab, Sweden.
Vise andre og tillknytning
(engelsk)Manuskript (preprint) (Annet vitenskapelig)
Emneord [en]
Giardia, HCMPs, interactions, host-parasite interactions, infection, parasite, RNA-seq
HSV kategori
Identifikatorer
URN: urn:nbn:se:uu:diva-381936OAI: oai:DiVA.org:uu-381936DiVA, id: diva2:1305311
Tilgjengelig fra: 2019-04-16 Laget: 2019-04-16 Sist oppdatert: 2019-04-19
Inngår i avhandling
1. Studies of Giardia-host interactions: role of cysteine-rich surface proteins.
Åpne denne publikasjonen i ny fane eller vindu >>Studies of Giardia-host interactions: role of cysteine-rich surface proteins.
2019 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Giardia intestinalis is a eukaryotic parasite that colonizes the small intestine of humans and animals causing the diarrheal disease known as giardiasis. This parasite is not invasive and does not internalize into host cells but it rather attaches to the brush border surface of the small intestine disrupting the epithelial barrier. Giardia causes around 280 million symptomatic infections in humans every year, while it can also cause chronic and asymptomatic infections. Giardiasis is a multifactorial disease but only few factors that directly contribute in the pathogenesis and virulence of the disease have been identified. G. intestinalis has eight genetic groups, but only two of them (A and B) are known to infect humans.

In this thesis, whole genome sequencing was performed for two human assemblage A isolates (AS175 and AS98) and were compared to assemblage A isolate WB genome (Paper I). Genome-wide variations were identified among the three isolates including isolate-specific coding sequences and high level of nucleotide diversity of multi-gene families such as VSPs and HCMPs.

We further used an in vitro model for parasite interaction with host intestinal epithelial cells (IECs) to study the interplay between Giardia and the human host. We have identified the major Giardia excretory-secretory products (ESPs) released by two Giardia isolates (WB and GS) when they interact with the Caco-2 IECs (Paper II). Wide changes in the transcriptome (Paper III) and the proteome (Paper IV) of the parasite (WB isolate) and the host IECs have been studied giving us a further understanding of the parasite-host interactions. An understudied gene family (HCMPs) was studied and further characterized during interactions in both RNA and protein level (Paper III, IV).

In conclusion, the thesis has provided a further understanding of Giardia-host interactions in vitro and the molecular mechanisms involved.

sted, utgiver, år, opplag, sider
Uppsala: Acta Universitatis Upsaliensis, 2019. s. 85
Serie
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1816
Emneord
Giardia, intestinal parasite, parasite infection, host-parasite interaction, virulence factors, HCMPs, cysteine-rich proteins, secretome, proteome
HSV kategori
Identifikatorer
urn:nbn:se:uu:diva-382071 (URN)978-91-513-0670-4 (ISBN)
Disputas
2019-06-14, A1:111a, Husargatan 3, Uppsala, 09:15 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2019-05-24 Laget: 2019-04-19 Sist oppdatert: 2019-06-18

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